CA1054937A

CA1054937A - Antipregnancy vaccine

Info

Publication number: CA1054937A
Application number: CA225,101A
Authority: CA
Inventors: Gursaran P. Talwar
Original assignee: All India Institute of Medical Sciences AIIMS
Current assignee: All India Institute of Medical Sciences AIIMS
Priority date: 1975-01-28
Filing date: 1975-04-21
Publication date: 1979-05-22
Also published as: IL47177A0; US4161519A; DK145283C; SE7505411L; NO751680L; AR217615A1; NO145083B; CH614626A5; SE7807571L; FI54997B; SE432526B; NO145083C; DK145283B; DE2518546A1; SE432706B; DK206975A; NL7505412A; FI54997C; FI751746A; IL47177A

Abstract

A vaccine comprising a conjugate of an antigenic carrier with beta sub-unit of human chorionic gonadotropin, or derivatives or fragments thereof, purified by procedures which minimize cross-reactivity with LH, such as immunosorption with heterologous LH antiserum prior to conjugation, and a process for making the vaccine. In addition, a process for preventing pregnancy using said vaccine is described.

Description

* * * * * * * * * * *

The present invention relates to a vaccine for pre--~i 10 venting preynancy, to a method of preparing the vaccine and to the method of using the vaccine for preventing pregnancy.
A variety of methods for controlling fertility and for preventing and terminating pregnancy are known. A variety of approaches such as condomes, IUCD's, mechanical devices, spermi-cidal creams and jellies, foam tablets, oral pills, etc. are currently available as a prevention against pregnancy. These methods, though effective to a variable extent, also have limita-tions. Most of them require constant motivation on the part of the user. Some require specialized medical attention.

. :

:' :

: . . . .. . . . .. . . . . . .. . .. . .

\

The development of immunological methods of con-trolling fertility has been the subject of experimentation going back at least to the beginning of this century. A number of approaches are described by Albert Tyler in "Approaches to the Control of Fertility Based on Immunological Phenomena," Journal of Reproduction and Fertility, (1961)2, pages 473-50~

.
Among the approaches followed in recent years by investigators have been immunization with placental, fetal materials, and hormones. To date, however, to Applicant's knowledge, there has been no effective antifertility vaccine developed. The present approach is based upon eliciting anti-body response by active immunization to a hormone critical to establishment and maintenance of pregnancy.
It is an object of the present invention to provide an antipregnancy vaccine.
It is a further object of the present invention to ., .
provide a process for preventing pregnancy in mammals including humans utilizing vaccines which are free of significant adverse side-reactions.
It is a further object of the present invention to provide a method for preparing an antipregnancy vaccine which produces antibodies with minimal cross-reaction with other endogenous hormones at physiological levels.
It is a further object of the present invention to provide a process which prevents pregnancy using a vaccine that can be administered by paramedical auxiliary person~nel and which requires only periodic supplemental treatment. ~;

~ ,.

.
~2--;, - , . ~: -- , . . .
, .

3~
It has now been found that a vaccine for the preven-tion of pregnancy is provided by forming a conjugate of a sub-ject-compatible immunogenic carrier with a preparation of beta sub-unit of hCG, the preparation being free of determinants capable of reacting with high affinity with anti LH sera.
Specifically, antipregnancy vaccines are obtained with conjugates of said carriers with a substance selected from the group con-sisting of (i) C-terminal fragments of the beta sub-unit of human chorionic gonadotropin, having from 30 to 38 amino acid residues, ` (ii) the nitro derivative of the beta sub-unit of hCG, and (iii) the immunochemically purified beta sub-unit of hCG, all substances free of determinants capable of reacting with high affinity with anti LH sera. The term immunochemically purified, in this context, includes treatment which removes components of the beta sub-unit preparation which, if retained, ~ would cause formation of antibodies that would cross-react with ; 20 endogenous LH. The vaccine of the present invention is sub-stantially free of cross reaction with other endogenous hormones, such as the pituitary hormones required for normal function of reproductive organs.
In accordance with one preferred embodiment of the invention, the beta sub-unit is separated from the alpha sub-unit of human chorionic gonadotropin in accordance with known procedures, is thereafter chemically purified by known means, and then ilmuro~hemically purified using a heterologous anti ;

~: ,. , . : . . . .

~5~3'7 LH immunosorbent. The vaccine in a preferred ~orm is a con-~ugate of a chemically and immunochemical urified form of beta sub-unit of human chorionic gonadotropin with tetanus toxoid in molecular proportions of about 1:5 to about 1:20.
The conjugation takes place in the presence of a condensing agent such as glutaraldehyde or ethyl aminopropyl carbodiimide to obtain a covalently linked conjugate. The conjugate is thereafter subjected to the conventional steps of dialyzing, sterility control and precipitation to form the vaccine.
In another aspect this invention provides such a process for preparing a vaccine for the prevention of pregnancy which comprises the steps of obtaining a chemically pure beta sub-unit of hCG, reacting said chemically pure beta sub-unit with an LH immunosorbent to free the preparation of determinants capable of reacting with high affinity with anti LH sera, and condensing the thus treated beta sub-unit preparation with a subject-compatible immunogenic carrier to obtain a vaccine. Preferably in this process the LH
immunosorbent is prepared by thoroughly dialyzing rabbit , 20 antiovine L~ sera against 100 mM phosphate buffered saline -~ at pH 7. 2, polymerizing the antisera by standing at room temperature for 3 hours with the addition of glutaraldehyde, homogenizing the polymerized material followed by washing with phosphate buffered saIine until the washings are free of non-polymerized free protein, washing thehomogenate with a 200 mM glycine HCl buffer at pH 2.2, neutralizing the homogenate with K2HPO4 solution, and equilibrating the immunosorbent thus produ~ed with phosphate buffered saline.
In a further preferred embodiment the treated beta sub-unit is condensed with tetanus toxoid in the molecular proportions of 5:1 to 20 1 in the presence of a condensing agent and the resulting product is subjected to the steps of dialyzation and ~ - 4 ~, . . .. . . .

:L6~591L5~3~7 precipitation on alum to form a vaccine.
In another embodiment the present invention provides a process for preparing a vaccine for the prevention of pregnancy which comprises: obtaining chemically pure beta human chorionic gonadotropin (hCG), reacting said chemically ; pure beta hCG with rabbit antiovine LH immunosorbent to obtain a beta hCG preparation which is free of determinants capable of reacting with high affinity with anti LH sera, and condens-ing the thus treated beta hCG preparation with a subject- ~.
compatible immunogenic carrier to obtain a vaccine. In a preferred aspect the immunosorbent lS prepared by thoroughly dialyzing rabbit antiovine LH seraagainst 100 mM phosphate -~
buffered saline at pH 7.2, polymerizing the antisera-by standing at room temperature for 3 hours with the addition of ~.
glutaraldehyde, homogenizing the polymerized material followed by washing with phosphatide buffered saline until the washings : .
are free of non-polymerized free protein, washing the homo-genate with a 200 mM glycine HCl buffer at pH 2.2, neutral-izing the homogenate with K2HPO4 solution, and equilibrating 2Q the immunosorbent thus produced with phosphatide buffered ..
saline. . -In another aspect the present invention provides a vaccine comprising a conjugate of a subject-compatible :
, immunogenic caxrier and a preparation of beta sub-unit of human chorionic gonadotropin, the preparation being free o :
determinants capable of reacting with high affinity with anti ~' LH sera, whenever prepared or produced by the process of claim 1, 5 or 8, or by an obvious chemical equivalent thereof~
- In a further aspect the present invention provides :.
a vaccine comprising a conjugate of a subject-compatible immunogenic carrier and a substance selected from the group consisting of C-terminal fragments of the beta sub-unit of ~ - 4a -', ,~, ~ ' .:

3~
human chorionic gonadotropin, having from 30 to 38 amino acid residues, the nitro derivative of the beta sub-unit of hCG, and the immunochemically purified beta sub-unit oE hCG, each substance being free of determinants capable of reacting with high affinity with anti LH, whenever prepared or produced by the process of claim 24, or by an obvious chemical equivalent thereof.
As known in the art, placenta is a tissue which develops only in pregnancy; it is not a normal part of the non-pregnant female. The vaccine of this invention is capable of counteracting the establishment and maintenance of pregnancy.
In humans, for example, a critical hormone, human ch~rionic -~gonadotxopin is produced by the trophoblasts on about the - eighth day after fertilization. This hormone supports, at this stage, the corpus luteum and ensures the receptivity of the uterus. The hormone may also protect the blastocyst by other means. Its neutralization results in the onset of menstruation, thus preventing the maintenance of pregnancy.
Human chorionic gonadotropin (hereinafter sometimes referred to as HCG) is a glyco-proteinic hormone composed of

2 su~-units: alpha and beta. The alpha sub-unit is nearly identical with the respective alpha sub-units o~ the hormones ., s 30 : :' ..

- 4b -;;., ~:

. ~ , . , . :. , ~`3~

Thyroid Stimulating Hormone (TSH), Follicle Stimulating Hormone (FSH), and Luteinizing Hormone (LH). The beta sub-unit of the hormone, in each case, ascribes principally the hormonal specificity.
The beta sub-unit of hCG is a polypeptide of 146-147 amino acids with 3 out of 5 carbohydrate residues in the C-terminal end. A portion of the beta sub-unit bears amino acid homologies with the beta sub-unit of LH. The beta hCG pre-pared by dissociation of highly purified hCG has a fair degree of microheterogeneity. The protein resolves into 8 bands by electrophoresis on analytical polyacrylamide gels. This micro-heterogeneity is observed in commercially available hCG, which is prepared from pregnancy urine. The microheterogeneity may be due to a variable degree of cleavage and/or modification of the molecule during its passage through various tissues.
hCG as a whole molecule or the beta sub-unit of hCG~, when injected into women, does not elicit the formation of -antibodies capable of neutralizing the activity of hCG. It can, however, be made antigenic by coupling it with strongly anti-genic haptens. Diazotized sulfanilic acid derivatives have been tried and it has been demonstrated that anti hCG antibodies are induced in postmenopausal women. This is described by . :
Vernon C. Stevens and C. Deans Crystle in "~ffects of Immuniza-tion with Hapten-Coupled HCG on the Human Menstral Cycle", Obstetrics and Gynecology, Vol. 42, No. 4, October, 1973.

However, subjectshad a depression or obliteration of mid-cycle `- ' .

LH peaks, suggesting to the investigator that the antibodies cross reacted in vivo with endogenous LH. Such a cross-reaction with a pituitary hormone required for normal function of reproductive organs is undesirable and potentially hazardous.
It is important according to the present invention that the beta sub-unit of hCG used in the reaction mixture be highly purified both chemically and immunochemically. The chemical purification of the beta sub-unit may be effected in any manner known in the art, such as the procedure described 10 in Morgan, F. J. and R. E. Canfield, Endocrinology 88, 1045, 1971 and F. J. Morgan, R. E. Canfield, J. L~ Vaitukaitis, and G. T. Ross, Endocrinology 94, pages 1601-1605, 1974. Another suitable method of obtaining a chemically pure beta sub-unit of hCG is described b~ Swaminathan, N. and Bahl, O. P. in "Dissociation and Recombination of Subunits of Human Chorionic Gonadotropin", Biochem. Biophys. Res. Commun., Vol, 40, page 422, (1970). However, these chemical purification procedures are not sufficient to remove determinants capable of reacting with high affinity with anti LH sera.
The immunochemical purification step consists of dissolving the chemically purified beta sub-unit of human chorionic gonadotropin in a physiological solvent, such as phosphate buffered saline, and stirring the solution at room temperature for a controlled length of -time with an immuno-sorbent, such as rabbit antiovine LH. The treatment with a : ' , ~ -, ::

.

- , : . . . , . , . - . : . , , ,, : . ~ . :. , ~3~
heterologous anti LH immunosorbent, under carefully controlled conditions, is designed to remove those parts of the prepara-tion which have determinants capable of reacting with high affinity with anti LH sera. The immunosorbent used in the process of this invention may be selected from any of several heterologous LH antisera such as rabbit antiovine L~I, monkey antiovine or anti bovine LH. The stirring is effected at a temperature of, for example, 25C, for periods of from 1 to 60 minutes or more, depending upon the temperature, the nature of the particular batch of beta sub-unit of hCG and the potency of immunosorbent used. The optimum condition should be deter-mined for each sample of beta sub-unit preparation and for each immunosorbent. The preparation is thereafter subjected to the step of centrifugation; the precipitate is discarded and the filtrate contains chemically and immunochemically purified beta sub-unit of human chorionic gonadotropin.
The C-terminal fragments of the beta sub-unit may be prepared by enzyme cleavage of beta sub-units of hCG or by synthesis of the C-terminal peptides by the standard procedures of Merrifield, R. B., Journal of Am. Chem. Soc., Vol. 86, 304, 1964. Suitable methods utilizing enzyme cleavage are described in Bahl et al., Biochem. Biophys. Res. Commun. 48, 416, 1972, and Morgan et al., Mol. Cell, Biochem. 2, 97, 1~73.
The nitro derivative of beta sub-unit of hCG may be ; prepared by the process described in Sokolousky et al., B~ochemistry 5, pages 3582-3589 (1966).
, .

., , ':

:

These preparations are subjected to -the immunochemical purification step to exclude determinants capable of reac~ing with high affinity with anti LH sera.
The term "high affinity" as used according to this invention describes a characteristic o~ those moieties whlch combine with the anti LH sera at a rapid rate at the temperature ; used. The anti LH sera for this purpose is preferably raised to heterologous LH.
The preparakion of beta sub-unit of human chorionic ; 10 gonadotropin free of determinants capable of reacting with high affinity with anti LH sera is then reacted with a subject-compatible, immunogenic carrier such as tetanus toxoid in desired proportions using a condensing agent, thus conjugating the beta sub-unit with the carrier. Suitable carriers for use in preparing the vaccine of the present invention include tetanus . toxoid, polymerized flagellin, KLH (Keyhole Lympet Hemocyanin), : dextran, heat or irradiation inactivated polio and yellow fever : viruses, heat or irradiation inactivated typhoid and para-.1 typhoid vaccines, cholera toxoid, BCG, antiplasmodium vaccine, :
pertussis and diphtheria toxoid and biodegradable polymers, such as polylactic acid, polyglycolic acid, collagen, synthetic polypeptides, and polynucleotides. :
In accordance with one of the preferred embodiments of the present invention~ the reaction product is a complex polymer of tetanus toxoid and the chemically and immunochemically purified beta sub-unit of human chorionic gonadotropin conjugated covalently in the molecular proportions ~rom about 1:5 to about . ~' .
.
',', -8- -~

: :: . .. . : ~

1:20 in the presence of a condensing or coupling agent such as glutaraldehyde or l-ethyl-3-(3-dimethyl aminopropyl) carbodi-imide.
When the condensing ayent consists of l-ethyl-3-(3-dimethyl~aminopropyl) carbodiimide, purified tetanus toxoid and beta sub-unit of human chorionic gonadotropin is admixed in molecular proportions within the range given above together with the condensing agent and the mixture is then incubated at a temperature not exceeding 25C, and for a period not exceeding six hours depending upon the temperature. I-t is preferred that the reaction is carried out at a temperature not exceeding 10C and for a period of 4 to 6 hours.
When the condensing agerlt consists of glutaraldehyde, purified tetanus toxoid and beta sub-unit of human chorionic ;~
gonadotropin is admixed in the desired molecular proportions together with the condensing agent and the mixture is then incubated at a temperature not exceeding 37C and for a period not greater than six hours. Preferably, the step of incubation is carried out at a temperature of about 20 to about 30C
and for a period of 2 to 4 hours.
In the embodiment using -the C-terminal fragments of B-hCG, it is combined with the tetanus toxoid in the molecular proportions of toxoid to fragments preparation ranging from 1:10 to 1:100, preferably 1:4-0. wfien t~he nitro derivative is used in the conjugate, it is used in the molecular pro-portion, toxoid to derivative, of about 1:5 to about 1:20.

, . .
, ~ .
" . .
....
, -9_ 1.. ,' . .'- ". .
.

, . . . . .,. , . , ,. . , ~:

The compound so o~tained is thereafter dialyzed thoroughIy against phosphate buf~er saline pH 7.2r 0.005 M in cold~ e.g. 5C, and collected in a sterile tube using a millipore filter and syringe assembly, using saline for transfer.
The prepa~ation is then subjected to the step of sterility testing, in a manner known as such in the art, to ensure the absence nf micro-organisms.
; Subsequent to the step of sterilization~ the preparation is precipitated on alum, using sterile potash alum, AlK(S04)2 12H20r an~ sterile sodium carbonate solutions. Instead of alum, any other suitable adjuvants may also be used. However, it has bPen found that precipitation on alumina protects the active substance from adsorption on glass containers and also pL-~vides a long lasting response. ~he vaccine is preferably ~3ed as an emulsion with vegetable o:ils at the time of injection, ! and this fur~her improves antigenicity.
Further objects and advantaqes of this invention will be more apparent from ~he ensuing de~cription relating to Examples for the prep~ration and use of the vaccine.
2 0 BRIE:F DESCRIPTION OF T~IE FIGURES

Figures 1 and 2 Show the reactivity of the respective prPpara~ions with the anti hCG and anti LH serum ; before and a~ter the immunosorption purification step.
.
Figure 3 Shows the rise and fall of antibody titers i~ a goat ater injection o the vaccine and aft~r a second boostex injection.

Figur~ ~ ShGws the ~iological properties of the anti (Beta-hC~-T~) serum and h~w it was effective for inhibition of the hCG induced ~n~ro~se o~ t~ ventral pr~state we~ight ; 30 in i~m~ture ra~s.
:
- 10 - , , .

Figure S Shows that the anti (Beta-hCG-TT) serum, --- prevents binding of hCG to receptors in membrane preparations ~rom the testes and ovaries~

Figures 6, 7 and 8 Show the ~esponse of three human ~ volunteers that received the vaccine o~ the present invention.

; Figure 9 Shows the ability of the antibodies from one human volunteer to block the binding of hCG to corpus luteum preparations in a radioreceptor assay.

Figure 10 Shows the ability o~the antibodies ..
from o~e hum~n volun~eer to bind with hC~i n vitro.

EXAMPLE I
- ~ .
An antipregnancy vaccine was prepared by the following procedure: :
A.

Chemical Purification of Beta sub-unit of human . ~`
Chorionic Gonadotropin . Purified human chorionic gonadotropin was prepared ,,!, from crude hCG by the method of Canfield e~ al. It was :
incubated at 40C for 1 hour with 8 M freshly prepared urea ;~ 20 solution that had been purified on a mixed cation-anion exchange ;~
; resin column, specifically a bed of equal quantities of `::
** ** . : .
Dowex-l X-8 and Dowex-50 X-4, 200 mesh. The column size was 2 x 40 cmO In the presence of urea, the beta sub-units are dissociated and then separated by the method described by .. .. ..
*Canfield et al. Recent Progr. Horm. Res. 27:124, lg71 **Dowex i~ a trademark of Dow Chemical Company for a seri~s of proprietary synthetic ion-exchange xesins O
.
` 30 ., ' ~

., . , . , ~ .

Morgan and Can~ield, Endocrinology 88, 1045, 1971. In accordance with this process, the mixture is passed through ion exchange columns and Sephadex columns repea,tedly to obtain the dissociated beta sub-unit with minimal contamination of the whole human chorionic gonadotropin molecule, or its alpha sub-unit.
B.

Immunochemical puri~ication of beta sub-unit of human chorionic gonadotropin , From the beta sub-unit of hcG prepared in step Ao above, micro samples were taken. A seriçs of 100 ug samples, 5 in number, of the beta sub-unit o~ hCG were each dissolved in 0~1 ml of 100 ~ sodium ~hosphate (NaH2PO4 ana Na2~PO4) bufered saline tO.~5~ NaCl) (PBS) (pH 7.2). Each sample was stirred at room temperature ~.5C) with an immunosorbent ,o* rabbit antio~ine L~ prepared in the manner described in C. infra. Stirring~times varied from 1, S, 10, 20 and 30 1~ minutes. ~fter the mi~ing, the tubes were centrifuge~ ' for 10 minutes at 0-4~C. The supernatent from each t~e . 20 was tested for reactivity against anti hCG and anti L~
By this test the appropriate conditions for purifying the entire batch of be~a sub-unit o hCG prepared in A~ were determined to be 39 minutes of stirring at 25C~ The selection ', of 30 minutes was based on the observation that the sample provided a supernatent with minimum level of reactivity with LH o *Sephadex (trade mark) is a three dimensional macroscopiC network of dext~an substances bonded or cross~linked to~eth~r, being capable of absorbing water with swelling and is commercially available from AB Pharmacia, Uppsala, Sweden.

, - 12 - ~
. ~ :

. . .. . .

The characteristics of the beta hCG of the . purified beta sub-unit of hCG prepared in A. above ~as compared with the immunochemically purified beta sub-unit preparation prepared above in B. Figures 1 and ~ of the ~.
attached drawings show the reactivity of the respective prep-arations with anti hCG and anti LH serum before (Figure 1) and after (Figure 2) the immunosorption purification step. The absorption step also improves the microheterogeneity o~ the preparation. Referring to Figures 1 and ~, the beta hCG
~reparations were sub~ected to electrophoresis in 7.5~
: ~nalytic~l polyacrylamide gels at pH ~.0, A set o~ the ;:
gel was stained ~or densimetxic measurements and another iaentical gel was transversely sliced in~o about 2.5 mm - thick segments. Proteins from eac:h segment were eluted . .-~-0.9%--sodium chlo~ide--solution. In ea~h o~Figures l.and..
2, the curve ( - _ ) represent:s proteins measured by densitometry; the curve ~ represents protei~s ., comp~ting with beta hCG125I for bi.nding with anti beta hCG
serum;-and the curve o~ o represents.pro~eins..competing . ..
~, 20 with hL~ ~ZsI or binding with---anti hLH serum~ will be no~ed that----~he-.immuno~hemical-l-~-purified preparation shows--a sub- -~tantial redu~ion--in reac~ y with an~i hLH serum.
It may--be-noted--that---the.optimum conditions-~:of time of stirring and temperature are determined by the---nature of--the particulas batch of beta su~-unit and the ; potency of the immunosorbent used,-- I-t is--recognized~t~at the latter varie3 wi~h the titses o~--the~a~ti-ser~m-used and with:-storage period of the~-immunosorbent.
.

~ 13 -~ ; . : , : , .. . . . .

. C..
PREP~RATION OF In~unosorbent 10 ml of antiserum (rabbit antiovine LH) obtained by immunization of rabbit antiovine LH is dialyzed thoroughly against PBS at 0-4C and pH i5 adjusted to pH 5 with l M
sodium acetate buffer. 3 ml of a 2.5% aqueous solution of glutaraldehyde is added gently with stirring. The reaction mixture is allowed to stand at room temperature (Z5~) for 3 hours. The polymerized material i5 homogenized * **
10 in a Potter ElveJhem homogenizer with Tef~on pestle. Xt is washed with PBS repeatedly till free of 280 my absorbing ~aterial ~on-polymerized free pro~ein). It js then washed with 200 ~ glycine - HCl buffer pH2.2 and neutralized with 2 M K2HPO4 s~lution and ~inally equilibrated with PBS. Thc immunosorbent is stored at 4C with 0.1~ ~a azide as a pre-servative in glycine HCl buffer pending the follow-up.
, D~

PREPAR~TION OF vaccine using l-ethyl-3-(3-dimethyl-~minopropyl) carbodiimide as condensin~ a~ent_ 20 Preparation (for 40 dosesi~

3.~ mg betcl sub-unit of hCG~ as prepared in B. abo~e was mixed with ~00 L~ tetanus toxoid ~1500 Lf/mg protein N) and 16 mg o~ 1-ethyl-3-~3-dimethyl-aminopropyl3 carbodiimi~e in 6.5 ml o~ PB5, and the solution was incubated at 10C
., .

*~he Potter ElveJhem (~rade mark) homogenIzer is a commercially available all glass homogeniz~r.
**Teflon is a trademaxk of DuPont de Nemours &
Company for tetrafluoroethylene polymer.
' - 13(a) -., . , ,. , , : :

3~ 1 for 5 hours with occasional shaking. The` molecular proportion of tetanus toxoid to beta sub-unit of hCG is abbut 1:10O The reaction pxoduct.was dialyzed thoroughly against pho'sphate buffer saline pH 7.2, 0.005 M in coldO The'product was collected '~ in a sterile tube usiny a millipore filter and syringe assembly, and using saline for transfer. The'product was tested for sterility on thioglycolate medium for seven days.
It was then.adsorbed in alum using sterile 10~ potash alum and sterile 10% sodium carbonate solutions. The vaccine was then distributed, sealed into ampules~ and sampled for pyrogens and '.
sterility. :~

PREPAR~TION OF vaccine using Glutaraldehyde as condensing agent -'P'repa'rat'ion' of 'l d'ose 80 ug of the beta sub-unit of hCG, as prepared in C. above was mixed with 1 dose tetanus toxoicl, 10 Lf units and 50 ul of 1~ aqueous glutaraldehyde. The mixture was .
incubated at 30C with occasional mixing for 3 hours, and then .. .. . .
dialyzed thoroughly against PBS pH 7.2, 0.005 M. The rest of the procedure is the same as that de!scribed in preparation Dl.
2 0 P ROPE RTI ES:
The vaccine prepared in accordance with Dl described above, hereinafter designated Beta-hCG TT was found to be immunogenic in mice, rabbits, goats, monkeys, as well as in the human female. The vaccine under D2 described above was found to have similar immunogenic characteristics in monkeys.

, : ' - ': . , . , ' : .
. ~ , , .
' ' ''~ ':

. ..
' ~
. - -,.: ,:
~ -14- ~
~PL' -.

Both gave rise to a fairly long lasting antibody response.
A single injection of the vaccine Dl given with Freund's adjuvant at multiple sites in the goat induced rising antibody titers for about 9 months af-ter which a plateau is reached. This was followed by a declining trend. A second booster injection in the declining phase lead to a sharp rise ~ in antibody titers. This is shown in Figure 3.
; The pattern of response in 10 experimental rhesus monkeys is found to be similar, although there are individual variations. This is also shown in Figure 3.
In examining the immunological properties of anti (beta-hCG-TT) serum, it was found that such serum raised in the goat or monkey reacts immunologically with whole hCG molecules.
The antiserum does not give a cross-reaction with human growth ; hormone (hGH), human prolactin, and human placental lactogen (hPL), FSH, TSH, or LH, as tested at the physiological and ; surge levels. Table 1 below summarizes the results.
,.

~ 20 ~ ~

:

.. .. . .

2 2 '~ 3 G

3~
o .
~ .
:~ ~ E~
. o I ~ o ~ o ~ ,~
E~ c) . - r~
F~ O d . A
~I h c X ~) d :~3 i-~ ~ ~ ~ o Lrl ~;r ~ o o o o o O O O ~ t 7 1~ ~1 Q ~ .
~ rl >t 1-- co O O O O O O O O O
~ C~ O 0~ .
~ r .~ . . . .
` 0 .~, O . , .
. . ..
:- . H ¢l ~ ~
.

~i 11 E~ ~) Cr~ ;:0 0 U~ 00 0 0 0 0 ~ ~ o o o o o ''` ~ ~ . ' ' ' .
~0 .
1 ~ ~¢~ .
I o ~1 ~1 .
~3 y r~ t~S O O O ~) O O O O O
E~ O ~ ~ i;~ ~ r~ o o o o o o o O
a~0~ 0 r~ 1 ~1 ~
) . ~ U
u~ . ~ io ~ ~ * ~c * ~e ~ U
. ~ ~e * )~ # *~ ~ Q) h tl7 tP b~ , U U .
O i~ u o . U o ~ o ~ ~1 r. r l r~l r-l O O Ot~ i O U O
. ~) ,c:
i_ ~ . U
a~ ~:
, i . . . . ~ :

The biological properties of the anti (Beta-hCG~TT) serum produced in the goat and monkey and the ability of the serum to neutralize hCG was tested in a number of systems. It was found effective for inhibition of the hCG induced increase of the ventral prostate weight in immature rats. Experimental data summarizing this work is shown in Figure 4. It also counter-acted the hCG induced rise in production of progesterone in corpus luteum slices ln vitro. This is shown in Table II.
TABLE II
EFFECT OF ANTI ~-HCG-TT ON PROGESTERONE
SYNTHESIS BY CORPUS LUTEU~
AdditionsP4 ug/g tis5ue 1. None79.4 i 15.2 2. + HCG (0.1 ug/ml)183.2 ~ 30.6 3. + HCG ~ anti ~-TT 105.4 ~ 23.4

4. + anti ~-TT 96.7 i 23.2 Mean ~ S.E. of 6 experiments It also prevents the binding of hCG to receptors in membrane preparations from the testes and ovaries. Experimental data showing this is summarized in Figure 5. All these -tests show that the antibodies raised against the compound Be`ta-hCG-TT
has the ability to not only react immunologically with the whole hCG molecules but also to neutralize its biological activity.
The (Beta-hCG-TT) vaccine preparation Dl was tested for safety as per British Pharmacopoeia requirements, and was found to give rise to no death in guinea pigs over the 4 weeks of observation period. It~was also devoid of acute and subacute toxicity as tested in two species, namely, the rodents and the monkey. Its tolerance has also been tested in 65 mice; 7 rabbits, and 13 monkeys for periods ranging up to 1 year and beyond. The . .

survival of the immunized animals was 100%. Other parameters such as hematology (hemoglobin, TLC/DLC, PCV, peripheral smear examination for platelets, reticulocytes) hepatic functions - (serum bilirubin, alkaline phosphatase, the transaminases GOT
and GPT, LDH isoenzymes, cholinesterase), Renal function tests, blood g]ucose, serum lipids, cholesterol, triglycerides, free fatty acids, and thyroid function tests were normal in immunized monkeys. The immunized monkeys did not develop immediate (Arthus) or delayed hypersensitivity to hCG.
Clinical Studies:
Six human female volunteers in the reproductive age group of 20-32 years were injected with either the vaccine pre-paration made according to Dl above, or with alternate placebos in blind trials. Since the object of these tests was to establish the immunogenei-ty of the vaccine of the present inventioll, the ~ six women selected for this stage of the testing were those `I which had previously undergone tubal ligation. The placebos, in all cases, gave no response. Each of the three subjects receiv-ing the vaccine elicited antibody response against both tetanus toxoid and human chorionic gonadotropin. Following a lag period which lasted from 4-6 weeks from subject to subject, a progres-j sive rise in antibody titers followed. Antibodies were also ' elicited simultaneously to tetanus.
;l Figures 6, 7, and 8 of the attached drawings show the response of the three human volunteers that received the vaccine.
Mrs. K. W., Figure 8 of the drawings, was 30 years old with 5 pregnancies and 4 living children~ The rise o~ antibodies reacting against hCG is shown by the (o ----o) curve, and the anti tetanus antibody by the ~o o) curve. The anti hCG
titers attained a plateau and have remained at this level at ' about 9 months after injection. The tests axe continuing. The date of menses are indicated by an X mark at the top of the graph. The subject has been menstruating regularly. The endo-~ metrium biopsy which was taken at 150 days, showed evidence of -~ ovulation~ which was confirmed by vaginal cytology and by the progesterone levels in the secretory phase of the cycle.
The second subject Mrs. A. M., Figure 7, was 32 years old, with 3 pregnancies and 2 live children. Figure 7 shows a similar type of immune response kinetics for Mrs. A. M. The endometrium biopsy in her case, as also the vaginal cytology and progesterone levels, indicate the patency of ovulation.
The third subject, Mrs. N~ D. (Figure 6) was 30 years old with 5 pregnancies and 3 live children. Mrs. N. D. had been feeding a child during the first about 150 days of the study, whieh explains the lack of menses during this period. Immuniza-tion with the vaccine preparation d:Ld not produce any disturbance in lactation in spite of a fairly good immune response. She has , since started menstruating and has had normal menstrual periods.
Libido and other reproduetive functions in all 3 subjeets have been completely normal. Thorough clinical examina tion has shown no detectable abnormality of any type. Other parameters such as hematology, (hemoglobin, TLC/DLC, PCV, peri-pheral smear examination for platelets, reticulocytes), hepatic functions (serum bilirubin, alkaline phosphatase, the trans-aminases GOT and GPT, LDH isoenzymes, cholinesterase), Renal~
function tests, blood glucose, serum lipids, cholesterol, tri-; glycerides,free fatty acids, and thyroid function tests were normal and immunization has produeed no alteration in organ funetions and metabolism. The subjects have also shown normal response to insulin hypoglyeaemie test in -terms of produetion of eortisol and growth hormone.

;
'' :' -19- :;

The sera of these immunized women as well as of monkeys and goats hyperimmunized with the B-hCG-TT preparation has been examined by various tests to exclude possible auto-immune reactions against body organs. They were found negative for antinuclear antibodies/ antimicrosomal antibodies and were devoid of rheumatoid factor reactivity. This information is summarized below in Table 2.

~ ~ ~

'' ' ~' , '' . ~ .

,~ , .

~ ` ~

.
'~

-20- ;

, :. ; ..

.: :

~:

~o ~l ~ a) o ' ' ' ., O ~ ~ ~

' I ~ . ~ ~ ~0 ~
' ~ H ~ . ~ .Q ~ X ~
: ~1 ..,, ~ ~J ~ .IJ ~ h .~J
~g , . ~ ~, o ~ o ~r o ~ ~ r` ~ ~
Ei

5~ ~ 3 rd r~l ) Z
Q) ,~
.: : :.

~.. .
- 2 1~

':, -They were also found to be negative in their re-activity against human thyroid, parathyroid, adrenals, kidney, testes, and ovaries. The data establishing these facts are summarized in Table 3 below.

~', ', ' :, :` :
~. 20 : ` .
' .

:~ :

'., ~ :
.. . .
~ :

''' ,, :.

o ~
~ a) o ~ c o o u~
.,1 ~1 0 ~ 5~
o o ~ ~-3 o o ~
O Z (~ Q O O
' . :

.
:
X ~ '.`' ~ ~ .
' ~ ~ ~J
d ~ ~ 0 O ~
~ u~ O
a~ ,a o s~
u~ ~ E~ ~
~ E~ m E~ ~ ~ K E~ O
E~ .
O :
' ~ ~ ~:

. . ~ ~.
; a) ~

~ o h N N
~: 4_1 r-l rl ~1 rl . ~ ~ E E E E

.', .- ~
: : .
, ~ .
-23- ~ :

: . .
:::
'~ . ' They were also negative in their reactivity against -tissue substrates from mice, rabbit and baboon as summarized in Table 4.

~;
.

.
~ 20 ~ :

'` ~
.
.' '~

; ~ ~
- :
.~

:

3~
. .

-i: u~ a) a)~ a.) ' ~ ~ ~ ,, ~.,, o u7 ~ ~ a) ~ ~
.~, U~ ~ o ~~ o .,, ~ ,~
. s~ O a) ~ ~o ~ o ~ ~ ~ Z s~ ~ ~ Z ~ ~ :
O ~I N

~ ~ q ~q~ a,~ ~

:~ ~n u~ o . ~~1 H H 41 1 O E~ Ql I~

, e 'E~ .. ." " "
: ~ . .~
~, ~ ~ . ~ .. ~., :.
.~ ~ . .~
q ~, . ~ h q ~ h q ~' ';

. ,~ , .
. .

, ' -25- .`. .

"'''.''.

,, ,~, . ..

The antibodies generated in the human volunteers were found competent for neutralizing the biological activity of the whole hCG. Figure 9 of the attached drawings shows the ability of these antibodies from volunteer Mrs. N.D. to block binding of hCG to corpus luteum preparations in a radioreceptors assay.
The ability of these antibodies to bind with hCG in _ivo is ` demonstrated by the determinations reported in Figure 10 of the -; attached drawings.
The total duration of immune response to hCG in humans following primary immunization with four fortnightly injections of the uaccine is expected to be beyond one year, although individual variations may occur. Active immunization has not evoked undesirable reactions. The subjects have had normal menstrual cycles. There is evidence of ovulation. The kidney, liver, pituitary, cardiovascular, thyroid and other organ functions are normal and undisturbed.

`
Example I]
l A vaccine was prepared using the beta-hCG end fragment '~ (BEF) containing the last 32-33 amino acid residues, in place of -~
the immunologically purified beta-hCG used in and following the ` procedure outlined in Example I. In this Example, beta-h~G end fragment (BEF) comprising the last 32-33 amino acid residues, is covalently linked to tetanus toxoid in a ratio of 40:1 using l~ethyl-3-(3-dimethyl aminopropylj carbodiimide. The BEF is obtained from beta-hCG by papain cleavage, as described according to the method described above. Alternatively, the BEF may be synthesized chemically, according to known procedures.
Sufficient vaccine for 30 doses is prepared by mixing 2.5 m~ BEF with 300 Lf of tetanus toxoid and 15 mg of 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide, in phosphate buffer saline - :~ ; ; ': , ., pH 7.2 to final volume 5.0 ml. This is incubated at 4C for

6 hours with occasional mixing. The product is dialyzed agains-t PBS 7.2 in the cold for 48 hours with 5-6 changes of 1 litre buffer each~
The balance of the procedure, including sterilization and preparation of the vaccine, is the same as described in Example I.

Example III
.
A vaccine was prepared using a nitro derivative of the beta sub-unit of hCÇ, following the procedure outlined in Example I, Dl. The molecular ratio of the derivative to tetanus toxoid was 10:1. The balarnce of the procedure~ including sterili-zation and preparation of the vaccine, is the same as described in Example I.

.: ,... :`:
.

~ 20 ~

~.
` ' ,'' . ': ' : '.'.
, .,, .: ,.

Claims

The embodiments of the invention in which an exclusive property or privilege is claimed are defined as follows:

1. A process for preparing a vaccine which comprises the steps of (a) obtaining a preparation of beta sub-unit of hCG free of determinants capable of reacting with high affinity with anti LH sera, and (b) condensing the substance in (a) with a subject-compatible immunogenic carrier.

2. The process of claim 1, wherein the conjugate is precipitated on alum to form a vaccine and packaged.

3. The process as in claim 1 for obtaining a vaccine for the prevention of pregnancy which comprises the steps of (a) obtaining a substance selected from the group consisting of (i) C-terminal fragments of the beta sub-unit of human chorionic gonadotropin, (ii) the nitro derivative of the beta sub-unit of hCG, and (iii) the immunochemically purified beta sub-unit of hCG, the substances being free of determinants capable of reacting with high affinity with anti LH sera, (b) condensing at least one substance from (a) with a subject-compatible immunogenic carrier.

4. The process of claim 3, wherein the carrier is tetanus toxoid.

5. The process as in claim 1 for preparing a vaccine for the prevention of pregnancy which comprises the steps of (a) obtaining a chemically pure beta sub-unit of hCG, (b) reacting said chemically pure beta sub-unit with an LH immunosorbent to free the preparation of determinants cap-able of reacting with high affinity with anti LH sera, and (c) condensing the thus treated beta sub-unit preparation with a subject-compatible immunogenic carrier to obtain a vaccine.

6. The process of claim 5, wherein the LH immuno-sorbent is rabbit antiovine LH.

7. The process of claim 6, wherein the carrier is tetanus toxoid.

8. The process of claim 5, wherein the LH immuno-sorbent is prepared by (a) thoroughly dialyzing rabbit antiovine LH sera against 100 mM phosphate buffered saline at pH 7.2, (b) polymerizing the antisera by standing at room temperature for 3 hours with the addition of glutaraldehyde, (c) homogenizing the polymerized material followed by washing with phosphate buffered saline until the washings are free of non-polymerized free protein, (d) washing the homogenate with a 200 mM glycine -HCl buffer at pH 2.2, (e) neutralizing the homogenate with K2HPO4 solution, and (f) equilibrating the immunosorbent thus produced with phosphate buffered saline.

9. The process of claim 5, wherein the treated beta sub-unit is condensed with tetanus toxoid in the molecular proportions of 5:1 to 20:1 in the presence of a condensing agent and the resulting product is subjected to the steps of dialyzation and precipitation on alum to form a vaccine.

10. The process of claim 9, wherein the condensing agent is selected from the group consisting of glutaraldehyde, and a carbodiimide.

11. The process of claim 9, wherein the condensing agent is l-ethyl-3-(3-aminopropyl) carbodiimide and the con-densing step is conducted at a temperature not in excess of room temperature and for a period not exceeding about 6 hours.

12. The process of claim 11, wherein the temperature does not exceed 10°C.

13. The process of claim 10, wherein the condensing agent is glutaraldehyde and the reaction is conducted at a temperature not exceeding 37°C for a period not exceeding 6 hours.

14. The process of claim 13, wherein the reaction temperature is within the range of about 20-30°C.

15. A process as in claim 1 for preparing a vaccine for the prevention of pregnancy which comprises:
(a) obtaining chemically pure beta human chorionic gonadotropin (hCG);
(b) reacting said chemically pure beta hCG with rabbit antiovine LH immunosorbent to obtain a beta hCG
preparation which is free of determinants capable of reacting with high affinity with anti LH sera; and (c) condensing the thus treated beta hCG preparation with a subject-compatible immunogenic carrier to obtain a vaccine.

16. The process of claim 15 wherein the carrier is tetanus toxoid.

17. The process of claim 15 wherein the immunosorbent is prepared by (a) thoroughly dialyzing rabbit antiovine LH
sera against 100 mM phosphate buffered saline at pH 7.2, (b) polymerizing the antisera by standing at room temperature for 3 hours with the addition of glutar-aldehyde, (c) homogenizing the polymerized material followed by washing with phosphatide buffered saline until the washings are free of non-polymerized free protein, (d) washing the homogenate with a 200 mM glycine -HCl buffer at pH 2.2, (e) neutralizing the homogenate with K2HPO4 solution, and (f) equilibrating the immununosorbent thus pro-duced with phosphatide buffered saline.

18. The process of claim 15 wherein the treated beta hCG is condensed with tetanus toxoid in molecular proportions of 5:1 to 20:1 in the presence of a condensing agent and the resulting product is subjected to the steps of dialyzation and precipitation on alum to form a vaccine.--;

19. The process of claim 18 wherein the condensing agent is selected from the group consisting of glutaraldehyde and a carbodiimide.--;

20. The process of claim 18 wherein the condensing agent is l-ethyl-3-(3-aminopropyl) carbodiimide and the condensing step is conducted at a temperature not in excess of room temperature and for a period not ex-ceeding about 6 hours.--;

21. The process of claim 20 wherein the tem-perature does not exceed 10°C.--;

22. The process of claim 19 wherein the con-densing agent is glutaraldehyde and the reaction is con-ducted at a temperature not exceeding 37°C. for a period not exceeding 6 hours.--;

23. The process of claim 22 wherein the re-action temperature is within the range of about 20-30°C.--.

24. A process as in Claim 3 wherein the hCG
in component (a) has from 30 to 38 amino acid residues.

25. A process as in claim 24 wherein the carrier is tetanus toxoid.

26. A vaccine comprising a conjugate of a subject- , compatible immunogenic carrier and a preparation of beta sub-unit of human chorionic gonadotropin, the preparation being free of determinants capable of reacting with high affinity with anti LH sera, whenever prepared or produced by the process of claim 1, 5 or 8, or by an obvious chemical equivalent thereof.

27. A vaccine comprising a conjugate of a subject-compatible immunogenic carrier and a substance selected from the group consisting of (a) C-terminal fragments of the beta sub-unit of human chorionic gonadotropin, having from 30 to 38 amino acid residues.
(b) the nitro derivative of the beta sub-unit of hCG, and (c) the immunochemically purified beta sub-unit of hCG, each substance being free of determinants capable of reacting with high affinity with anti LH, whenever prepared or produced by the process of claim 24, or by an obvious chemical equivalent thereof.

28. The vaccine of claim 27, wherein the carrier is tetanus toxoid, whenever prepared or produced by the process of claim 25, or by an obvious chemical equivalent thereof.