CA2024892A1 - Blood collection method - Google Patents

Blood collection method

Info

Publication number
CA2024892A1
CA2024892A1 CA2024892A CA2024892A CA2024892A1 CA 2024892 A1 CA2024892 A1 CA 2024892A1 CA 2024892 A CA2024892 A CA 2024892A CA 2024892 A CA2024892 A CA 2024892A CA 2024892 A1 CA2024892 A1 CA 2024892A1
Authority
CA
Canada
Prior art keywords
phase
lighter phase
separated
whole blood
lighter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2024892A
Other languages
French (fr)
Other versions
CA2024892C (en
Inventor
Richard Lewis Columbus
Susan Melissa Atwood
Deborah Paula Freyler
Harvey John Palmer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eastman Kodak Co
Original Assignee
Eastman Kodak Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eastman Kodak Co filed Critical Eastman Kodak Co
Publication of CA2024892A1 publication Critical patent/CA2024892A1/en
Application granted granted Critical
Publication of CA2024892C publication Critical patent/CA2024892C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/15—Devices for taking samples of blood
    • A61B5/150007—Details
    • A61B5/150015—Source of blood
    • A61B5/150022—Source of blood for capillary blood or interstitial fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/15—Devices for taking samples of blood
    • A61B5/150007—Details
    • A61B5/150206—Construction or design features not otherwise provided for; manufacturing or production; packages; sterilisation of piercing element, piercing device or sampling device
    • A61B5/150221—Valves
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/15—Devices for taking samples of blood
    • A61B5/150007—Details
    • A61B5/150206—Construction or design features not otherwise provided for; manufacturing or production; packages; sterilisation of piercing element, piercing device or sampling device
    • A61B5/150251—Collection chamber divided into at least two compartments, e.g. for division of samples
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/15—Devices for taking samples of blood
    • A61B5/150007—Details
    • A61B5/150343—Collection vessels for collecting blood samples from the skin surface, e.g. test tubes, cuvettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
    • A61M5/178—Syringes
    • A61M5/31—Details
    • A61M5/32—Needles; Details of needles pertaining to their connection with syringe or hub; Accessories for bringing the needle into, or holding the needle on, the body; Devices for protection of needles
    • A61M5/3205—Apparatus for removing or disposing of used needles or syringes, e.g. containers; Means for protection against accidental injuries from used needles
    • A61M5/321—Means for protection against accidental injuries by used needles
    • A61M5/322—Retractable needles, i.e. disconnected from and withdrawn into the syringe barrel by the piston
    • A61M5/3221—Constructional features thereof, e.g. to improve manipulation or functioning
    • A61M2005/3228—Constructional features thereof, e.g. to improve manipulation or functioning the needle being retracted by a member protruding laterally through a slot in the barrel, e.g. double-ended needles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
    • A61M5/50—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests having means for preventing re-use, or for indicating if defective, used, tampered with or unsterile
    • A61M5/5086—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests having means for preventing re-use, or for indicating if defective, used, tampered with or unsterile for indicating if defective, used, tampered with or unsterile
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0858—Side walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0605—Valves, specific forms thereof check valves
    • B01L2400/0616—Ball valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

A method of use is disclosed of a device that causes phase separation of whole blood, using much lower centrifugal forces. As a result, leukocytes are separated from blood cells having specific gravities of 1.09 g/ml or higher. The method preferably uses a separation chamber arranged so that its long dimension or axis is parallel, not perpendicular, to the spin axis, and a valve that allows automatic removal of the lighter phase(s).
In one respect, the method separates a lighter phase from a heavier phase, for example, lymphocytes from whole blood, using a centrifugal force of reduced amount. In another aspect, DNA is extracted readily from the separated lighter phase since it is less likely that the lighter phase will be contaminated by erthrocytes as occurs in conventional separation techniques.
CA002024892A 1989-11-29 1990-09-07 Blood collection method Expired - Fee Related CA2024892C (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US44282689A 1989-11-29 1989-11-29
US442,826 1989-11-29
US524,401 1990-05-16
US07/524,401 US5032288A (en) 1989-11-29 1990-05-16 Blood collection method

Publications (2)

Publication Number Publication Date
CA2024892A1 true CA2024892A1 (en) 1991-05-30
CA2024892C CA2024892C (en) 1995-05-16

Family

ID=23758314

Family Applications (4)

Application Number Title Priority Date Filing Date
CA002013021A Expired - Fee Related CA2013021C (en) 1989-11-29 1990-03-26 Blood collection device
CA002024891A Expired - Fee Related CA2024891C (en) 1989-11-29 1990-09-07 Blood collection device
CA002024892A Expired - Fee Related CA2024892C (en) 1989-11-29 1990-09-07 Blood collection method
CA002029459A Abandoned CA2029459A1 (en) 1989-11-29 1990-11-07 Blood collection device

Family Applications Before (2)

Application Number Title Priority Date Filing Date
CA002013021A Expired - Fee Related CA2013021C (en) 1989-11-29 1990-03-26 Blood collection device
CA002024891A Expired - Fee Related CA2024891C (en) 1989-11-29 1990-09-07 Blood collection device

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA002029459A Abandoned CA2029459A1 (en) 1989-11-29 1990-11-07 Blood collection device

Country Status (5)

Country Link
US (1) US5032288A (en)
EP (2) EP0430355A3 (en)
JP (2) JPH03185356A (en)
CA (4) CA2013021C (en)
IE (2) IE904303A1 (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE462015B (en) * 1987-09-15 1990-04-30 Omega Medicinteknik Ab SETTING AND DEVICE CLEANING BLOOD CELLS
PL307164A1 (en) * 1992-07-13 1995-05-02 Pall Corp Automatic system for and method of processing biological fluids
US5610074A (en) * 1993-02-24 1997-03-11 Beritashvili; David R. Centrifugal method and apparatus for isolating a substance from a mixture of substances in a sample liquid
SE9301759D0 (en) * 1993-05-21 1993-05-21 Vincenzo Vassarotti CENTRIFUGAL METHOD FOR CONCENTRATING MACROMOLECULES FROM A SOLUTION AND DEVICE FOR CARRYING OUT SAID METHOD
AU708820B2 (en) * 1993-11-19 1999-08-12 Vivolution A/S Annular assembly for centrifuge device
ZA948564B (en) * 1993-11-19 1995-07-26 Bristol Myers Squibb Co Liquid separation apparatus and method
SE9700495D0 (en) 1997-02-12 1997-02-12 Omega Medicinteknik Ab Method and round bag system and centrifuge for blood treatment
SE516321C2 (en) 1999-05-31 2001-12-17 Gambro Inc Centrifuge for the treatment of blood and blood components
SE517032C2 (en) 1999-10-26 2002-04-02 Gambro Inc Method and apparatus for treating blood and blood components
ATE382382T1 (en) 2002-04-16 2008-01-15 Gambro Bct Inc SYSTEM AND METHOD FOR PROCESSING BLOOD COMPONENTS
US20040166551A1 (en) * 2003-02-24 2004-08-26 John Moulds Detection of agglutination of assays
JP5125680B2 (en) * 2007-03-29 2013-01-23 東レ株式会社 Separation chip and separation method
JP5876047B2 (en) 2010-07-19 2016-03-02 テルモ ビーシーティー、インコーポレーテッド Centrifuge for processing blood and blood components
US8317672B2 (en) 2010-11-19 2012-11-27 Kensey Nash Corporation Centrifuge method and apparatus
US8870733B2 (en) 2010-11-19 2014-10-28 Kensey Nash Corporation Centrifuge
US8556794B2 (en) 2010-11-19 2013-10-15 Kensey Nash Corporation Centrifuge
US8394006B2 (en) 2010-11-19 2013-03-12 Kensey Nash Corporation Centrifuge
US8469871B2 (en) 2010-11-19 2013-06-25 Kensey Nash Corporation Centrifuge
WO2015117007A1 (en) 2014-01-31 2015-08-06 Dsm Ip Assets B.V. Adipose tissue centrifuge and method of use
EP3648670B1 (en) * 2017-07-06 2023-01-25 Becton, Dickinson and Company Biological fluid collection device
CN113396004A (en) * 2019-01-04 2021-09-14 Fjm创新有限公司 Phase separation tube

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3841838A (en) * 1969-07-30 1974-10-15 Rohe Scientific Corp Centrifuge cups for automatic chemical analyzer
US3849072A (en) * 1972-04-25 1974-11-19 Becton Dickinson Co Plasma separator
US3935113A (en) * 1974-02-27 1976-01-27 Becton, Dickinson And Company Serum/plasma separator with centrifugal valve
US3941699A (en) * 1974-02-27 1976-03-02 Becton, Dickinson And Company Plasma separator with centrifugal valve
US3945928A (en) * 1974-02-27 1976-03-23 Becton, Dickinson And Company Serum/plasma separators with centrifugal valves
US3998383A (en) * 1975-07-16 1976-12-21 E. I. Du Pont De Nemours And Company Gradient separation apparatus
JPS52100661A (en) * 1976-02-17 1977-08-23 Hitachi Metals Ltd Travel type precipitate scraping device
US4202769A (en) * 1977-06-16 1980-05-13 Greenspan Donald J Method for separating serum or plasma from the formed elements of blood
US4487700A (en) * 1983-02-18 1984-12-11 Technicon Instruments Corporation Method and apparatus for separating lymphocytes from anticoagulated blood
US4640785A (en) * 1984-12-24 1987-02-03 Becton Dickinson And Company Separation of lymphocytes and monocytes from blood samples
FR2592170B1 (en) * 1985-12-20 1988-02-05 Guigan Jean METHOD AND DEVICE FOR DELIVERING A PREDETERMINED QUANTITY OF PLASMA FROM A BLOOD SAMPLE FOR ANALYSIS.
US4708710A (en) * 1986-03-27 1987-11-24 E. I. Du Pont De Nemours And Company Particle separation process
US4828716A (en) * 1987-04-03 1989-05-09 Andronic Devices, Ltd. Apparatus and method for separating phases of blood

Also Published As

Publication number Publication date
JP2989256B2 (en) 1999-12-13
CA2024891A1 (en) 1991-05-30
JPH03185356A (en) 1991-08-13
CA2024891C (en) 1995-05-09
CA2029459A1 (en) 1991-05-30
EP0430356A2 (en) 1991-06-05
CA2013021C (en) 1995-05-09
JPH03270748A (en) 1991-12-02
CA2024892C (en) 1995-05-16
CA2013021A1 (en) 1991-05-29
EP0430356A3 (en) 1992-01-02
US5032288A (en) 1991-07-16
EP0430355A3 (en) 1992-01-02
IE904304A1 (en) 1991-06-05
IE904303A1 (en) 1991-06-05
EP0430355A2 (en) 1991-06-05

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