CA2067203A1 - Method of cryopreserving musculoskeletal tissues - Google Patents
Method of cryopreserving musculoskeletal tissuesInfo
- Publication number
- CA2067203A1 CA2067203A1 CA2067203A CA2067203A CA2067203A1 CA 2067203 A1 CA2067203 A1 CA 2067203A1 CA 2067203 A CA2067203 A CA 2067203A CA 2067203 A CA2067203 A CA 2067203A CA 2067203 A1 CA2067203 A1 CA 2067203A1
- Authority
- CA
- Canada
- Prior art keywords
- cryopreserving
- cell
- glycosaminoglycan
- tissue
- musculoskeletal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title abstract 3
- 210000001519 tissue Anatomy 0.000 abstract 4
- 229920002683 Glycosaminoglycan Polymers 0.000 abstract 3
- 239000003795 chemical substances by application Substances 0.000 abstract 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 abstract 2
- 230000003833 cell viability Effects 0.000 abstract 2
- 238000007710 freezing Methods 0.000 abstract 2
- 230000008014 freezing Effects 0.000 abstract 2
- 229920001287 Chondroitin sulfate Polymers 0.000 abstract 1
- 210000000845 cartilage Anatomy 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 210000003041 ligament Anatomy 0.000 abstract 1
- 230000014759 maintenance of location Effects 0.000 abstract 1
- 239000003960 organic solvent Substances 0.000 abstract 1
- 210000002435 tendon Anatomy 0.000 abstract 1
- 238000010257 thawing Methods 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
Abstract
Disclosed herein is a method for cryopreserving musculoskeletal tissues, such as ligaments, tendons and cartilage, by placing such tissue in contact with a composition containing a cryopreserving agent comprising a cell-penetrating organic solute, which is preferably dimethylsulfoxide, and a glycosaminoglycan, which is preferably chondroitin sulphate, in an amount sufficient to cryopreserve the musculoskeletal tissue. The addition of a glycosaminoglycan to a cryopreserving agent comprising a cell-penetrating organic solute permits a broad range of cooling rates to be employed, rather than the very narrow ranges which are employed using a cryopreserving agent comprising a cell-penetrating organic solvent without the glycosaminoglycan. Also disclosed are a freezing schedule designed to maximize retention of tissue cell viability and biomechanical properties during and after the freezing process, and a thawing schedule which maximizes cell viability.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US431,153 | 1989-11-03 | ||
US07/431,153 US5131850A (en) | 1989-11-03 | 1989-11-03 | Method for cryopreserving musculoskeletal tissues |
PCT/US1990/006221 WO1991006213A1 (en) | 1989-11-03 | 1990-11-02 | Method of cryopreserving musculoskeletal tissues |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2067203A1 true CA2067203A1 (en) | 1991-05-04 |
CA2067203C CA2067203C (en) | 2000-01-11 |
Family
ID=23710715
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002067203A Expired - Fee Related CA2067203C (en) | 1989-11-03 | 1990-11-02 | Method of cryopreserving musculoskeletal tissues |
Country Status (5)
Country | Link |
---|---|
US (1) | US5131850A (en) |
EP (1) | EP0500788A4 (en) |
AU (1) | AU7740091A (en) |
CA (1) | CA2067203C (en) |
WO (1) | WO1991006213A1 (en) |
Families Citing this family (78)
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US6010692A (en) * | 1988-05-31 | 2000-01-04 | University Of Florida Research Foundation, Inc. | Method and composition for preventing surgical adhesions and tissue damage |
WO1992012722A1 (en) * | 1990-01-17 | 1992-08-06 | The Regents Of The University Of California | Antifreeze glycopeptide compositions to protect cells and tissues during freezing |
US8067149B2 (en) * | 1990-09-12 | 2011-11-29 | Lifecell Corporation | Acellular dermal matrix and method of use thereof for grafting |
US5336616A (en) * | 1990-09-12 | 1994-08-09 | Lifecell Corporation | Method for processing and preserving collagen-based tissues for transplantation |
US5192312A (en) * | 1991-03-05 | 1993-03-09 | Colorado State University Research Foundation | Treated tissue for implantation and methods of treatment and use |
US5891617A (en) * | 1993-09-15 | 1999-04-06 | Organogenesis Inc. | Cryopreservation of harvested skin and cultured skin or cornea equivalents by slow freezing |
ES2219660T3 (en) * | 1994-03-14 | 2004-12-01 | Cryolife, Inc | METHODS OF PREPARATION OF FABRICS FOR IMPLEMENTATION. |
ES2080687B1 (en) * | 1994-04-06 | 1996-08-16 | Criobarna S A | PROCEDURE FOR THE OBTAINING OF CRYO-PRESERVED VALVE HOMO-GRAFTES AND FOR THE DEFROSTING OF THEM. |
US6046379A (en) * | 1995-06-07 | 2000-04-04 | Stone; Kevin R. | Meniscal xenografts |
US5984858A (en) * | 1995-06-07 | 1999-11-16 | Crosscart, Inc. | Meniscal xenografts |
US5913900A (en) * | 1995-06-07 | 1999-06-22 | Corsscart, Inc. | Substantially native meniscal cartilage heterografts |
US6231608B1 (en) | 1995-06-07 | 2001-05-15 | Crosscart, Inc. | Aldehyde and glycosidase-treated soft and bone tissue xenografts |
US5865849A (en) * | 1995-06-07 | 1999-02-02 | Crosscart, Inc. | Meniscal heterografts |
US5902338A (en) * | 1995-09-15 | 1999-05-11 | Crosscart, Inc. | Anterior cruciate ligament heterograft |
US5782915A (en) * | 1995-09-15 | 1998-07-21 | Stone; Kevin R. | Articular cartilage heterografts |
US5944755A (en) * | 1995-09-15 | 1999-08-31 | Crosscart, Inc. | Articular cartilage xenografts |
US6210440B1 (en) | 1995-09-15 | 2001-04-03 | Kevin R. Stone | Anterior cruciate ligament xenografts |
US6110206A (en) | 1995-09-15 | 2000-08-29 | Crosscart, Inc. | Anterior cruciate ligament xenografts |
US6049025A (en) * | 1995-09-15 | 2000-04-11 | Stone; Kevin R. | Articular cartilage xenografts |
US5689961A (en) | 1996-01-30 | 1997-11-25 | Organogenesis Inc. | Ice seeding apparatus for cryopreservation systems |
US5879875A (en) * | 1996-06-14 | 1999-03-09 | Biostore New Zealand | Compositions and methods for the preservation of living tissues |
US6037116A (en) * | 1996-06-14 | 2000-03-14 | Biostore New Zealand, Ltd. | Compositions comprising betaine, sodium citrate and sodium chloride and methods for the preservation of biological materials |
US6114107A (en) * | 1996-06-14 | 2000-09-05 | Biostore New Zealand Limited | Composition comprising raffinose, TMAO, sodium citrate and methods for the preservation of living tissues |
US6361933B1 (en) | 1996-06-14 | 2002-03-26 | Biostore New Zealand Limited | Solutions for the preservation of tissues |
US5962213A (en) * | 1996-06-14 | 1999-10-05 | Biostore New Zealand Limited | Compositions and methods for the preservation of living tissues |
US6758865B1 (en) | 1998-03-06 | 2004-07-06 | Crosscart, Inc. | Soft tissue xenografts |
US6383732B1 (en) | 1999-02-11 | 2002-05-07 | Crosscart, Inc. | Method of preparing xenograft heart valves |
US6972041B1 (en) | 1998-03-16 | 2005-12-06 | Crosscart, Inc. | Bone xenografts |
EP1061866A4 (en) | 1998-03-16 | 2003-07-16 | Crosscart Inc | Bone xenografts |
US6933326B1 (en) | 1998-06-19 | 2005-08-23 | Lifecell Coporation | Particulate acellular tissue matrix |
US6630001B2 (en) | 1998-06-24 | 2003-10-07 | International Heart Institute Of Montana Foundation | Compliant dehyrated tissue for implantation and process of making the same |
US6497726B1 (en) * | 2000-01-11 | 2002-12-24 | Regeneration Technologies, Inc. | Materials and methods for improved bone tendon bone transplantation |
US6267786B1 (en) | 1999-02-11 | 2001-07-31 | Crosscart, Inc. | Proteoglycan-reduced soft tissue xenografts |
US20010036665A1 (en) * | 1999-03-18 | 2001-11-01 | Susan M. Young | Method of preparing cryogenically preserved adherent cell containing plate for tissue culture applications |
US6793677B2 (en) * | 1999-08-13 | 2004-09-21 | Bret A. Ferree | Method of providing cells and other biologic materials for transplantation |
US6352708B1 (en) | 1999-10-14 | 2002-03-05 | The International Heart Institute Of Montana Foundation | Solution and method for treating autologous tissue for implant operation |
WO2002014480A2 (en) * | 2000-08-16 | 2002-02-21 | Duke University | Decellularized tissue engineered constructs and tissues |
JP2004529711A (en) * | 2001-05-07 | 2004-09-30 | クロスカート インコーポレイテッド | Submucosal xenograft |
US7064187B2 (en) | 2002-11-26 | 2006-06-20 | Crosscart, Inc. | Substantially non-immunogenic injectable collagen |
AU2003302898A1 (en) * | 2002-12-11 | 2004-06-30 | Cryolife, Inc. | Radical retardant cryopreservation solutions |
US20070077237A1 (en) * | 2003-10-09 | 2007-04-05 | Udi Damari | Method for freezing, thawing and transplantation of viable cartilage |
US20080176205A1 (en) * | 2003-12-04 | 2008-07-24 | University Of Utah Research Foundation | Process and Formulation to Improve Viability of Stored Cells and Tissue |
EP1711214A1 (en) * | 2004-02-02 | 2006-10-18 | Interface Multigrad Technology (IMT) Ltd. | Device for directional cooling of biological matter |
EP1711053A2 (en) * | 2004-02-02 | 2006-10-18 | I.M.T. Interface Multigrad Technology Ltd. | Biological material and methods and solutions for preservation thereof |
DE602005019998D1 (en) * | 2004-06-07 | 2010-04-29 | Core Dynamics Ltd | PROCESS FOR STERILIZING BIOLOGICAL PREPARATIONS |
EP1778007A1 (en) * | 2004-08-12 | 2007-05-02 | I.M.T. Interface Multigrad Technology Ltd. | Method and apparatus for freezing or thawing of a biological material |
EP1850661A2 (en) * | 2005-02-22 | 2007-11-07 | Interface Multigrad Technology (IMT) Ltd. | Preserved viable cartilage, method for its preservation, and system and devices used therefor |
EP1909565A2 (en) * | 2005-08-03 | 2008-04-16 | Interface Multigrad Technology (IMT) Ltd. | Somatic cells for use in cell therapy |
US7766964B2 (en) * | 2007-03-30 | 2010-08-03 | Biomet Sports Medicine, Llc | In situ graft preparation for knee ligament reconstruction |
US20090181807A1 (en) * | 2008-01-16 | 2009-07-16 | Jason Miguel De Los Santos | Golfing aid |
US20090202978A1 (en) * | 2008-02-13 | 2009-08-13 | Ginadi Shaham | Method and apparatus for freezing of a biological material |
US8469779B1 (en) | 2009-01-02 | 2013-06-25 | Lifecell Corporation | Method for debristling animal skin |
US10328103B2 (en) | 2009-01-03 | 2019-06-25 | Ray C. Wasielewski | Medical treatment composition comprising mammalian dental pulp stem cells |
US8470308B2 (en) * | 2009-01-03 | 2013-06-25 | Ray C. Wasielewski | Enhanced medical implant comprising disrupted tooth pulp and tooth particles |
US20100233669A1 (en) * | 2009-03-11 | 2010-09-16 | Cryolife, Inc. | Bioburden-reducing antibiotic composition and method of use |
US20140286911A1 (en) | 2013-03-15 | 2014-09-25 | Allosource | Cell repopulated collagen matrix for soft tissue repair and regeneration |
US8475827B2 (en) | 2010-07-06 | 2013-07-02 | Cryolife, Inc. | Tissue implants for implantation and methods for preparing the same |
US8377143B2 (en) | 2010-07-06 | 2013-02-19 | Cryolife, Inc. | Tissue implants for implantation and methods for preparing the same |
US9375513B2 (en) | 2011-04-14 | 2016-06-28 | Lifecell Corporation | Regenerative materials |
US9089523B2 (en) | 2011-07-28 | 2015-07-28 | Lifecell Corporation | Natural tissue scaffolds as tissue fillers |
EP2793964B1 (en) | 2011-12-20 | 2019-03-20 | LifeCell Corporation | Sheet tissue products |
BR112014014975B1 (en) | 2011-12-20 | 2019-06-25 | Lifecell Corporation | A method of producing a fabric composition |
US9271821B2 (en) | 2012-01-24 | 2016-03-01 | Lifecell Corporation | Elongated tissue matrices |
EP2841116A1 (en) | 2012-04-24 | 2015-03-04 | Lifecell Corporation | Flowable tissue matrices |
AU2013290065A1 (en) * | 2012-07-11 | 2015-02-19 | Osiris Therapeutics, Inc. | Methods of manufacturing cartilage products |
ES2753156T3 (en) | 2012-07-13 | 2020-04-07 | Lifecell Corp | Improved adipose tissue treatment procedures |
EP3332816B1 (en) | 2012-09-26 | 2020-11-04 | LifeCell Corporation | Processed adipose tissue |
CA2899724A1 (en) | 2013-02-06 | 2014-08-14 | Lifecell Corporation | Methods for localized modification of tissue products |
KR102215401B1 (en) | 2013-02-22 | 2021-02-10 | 알로소스 | Cartilage mosaic compositions and methods |
CA2895140C (en) | 2013-03-15 | 2021-07-13 | Allosource | Perforated osteochondral allograft compositions |
WO2016154452A2 (en) | 2015-03-24 | 2016-09-29 | Osiris Therapeutics, Inc. | Compositions comprising meniscal tissues and uses thereof |
WO2017210109A1 (en) | 2016-06-03 | 2017-12-07 | Lifecell Corporation | Methods for localized modification of tissue products |
AU2017382173A1 (en) | 2016-12-22 | 2019-06-06 | Lifecell Corporation | Devices and methods for tissue cryomilling |
US11123375B2 (en) | 2017-10-18 | 2021-09-21 | Lifecell Corporation | Methods of treating tissue voids following removal of implantable infusion ports using adipose tissue products |
CN115252910A (en) | 2017-10-18 | 2022-11-01 | 生命细胞公司 | Adipose tissue products and methods of production |
EP3697462B1 (en) | 2017-10-19 | 2023-07-12 | LifeCell Corporation | Flowable acellular tissue matrix products and methods of production |
US11246994B2 (en) | 2017-10-19 | 2022-02-15 | Lifecell Corporation | Methods for introduction of flowable acellular tissue matrix products into a hand |
EP3976127A1 (en) | 2019-05-30 | 2022-04-06 | LifeCell Corporation | Biologic breast implant |
Family Cites Families (7)
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---|---|---|---|---|
US3033662A (en) * | 1957-08-01 | 1962-05-08 | Eastman Kodak Co | Gasoline with copolymeric lead stabilizer and a synergistic modification thereof |
GB1110576A (en) * | 1964-08-22 | 1968-04-18 | Yasuharu Kuwahara | Preservative for human and animal eyes |
US3444039A (en) * | 1964-12-14 | 1969-05-13 | Ambrose Harry Jesudasan Rajama | Freezable live cell diluent and process |
US4486416A (en) * | 1981-03-02 | 1984-12-04 | Soll David B | Protection of human and animal cells subject to exposure to trauma |
US4959319A (en) * | 1985-08-01 | 1990-09-25 | Skelnik Debra L | Process of corneal enhancement |
US5145769A (en) * | 1987-08-21 | 1992-09-08 | Cryolife Inc. | Method for cryopreserving blood vessels |
US4800429A (en) * | 1988-03-14 | 1989-01-24 | Motorola, Inc. | Auto sync polarity control circuit for use with monitor |
-
1989
- 1989-11-03 US US07/431,153 patent/US5131850A/en not_active Expired - Lifetime
-
1990
- 1990-11-02 AU AU77400/91A patent/AU7740091A/en not_active Abandoned
- 1990-11-02 CA CA002067203A patent/CA2067203C/en not_active Expired - Fee Related
- 1990-11-02 EP EP19910900625 patent/EP0500788A4/en not_active Ceased
- 1990-11-02 WO PCT/US1990/006221 patent/WO1991006213A1/en not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
EP0500788A4 (en) | 1992-11-19 |
CA2067203C (en) | 2000-01-11 |
AU7740091A (en) | 1991-05-31 |
WO1991006213A1 (en) | 1991-05-16 |
EP0500788A1 (en) | 1992-09-02 |
US5131850A (en) | 1992-07-21 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |