CA2078132A1 - A process for distinguishing nucleic acids on the basis of nucleotide differences - Google Patents

A process for distinguishing nucleic acids on the basis of nucleotide differences

Info

Publication number
CA2078132A1
CA2078132A1 CA2078132A CA2078132A CA2078132A1 CA 2078132 A1 CA2078132 A1 CA 2078132A1 CA 2078132 A CA2078132 A CA 2078132A CA 2078132 A CA2078132 A CA 2078132A CA 2078132 A1 CA2078132 A1 CA 2078132A1
Authority
CA
Canada
Prior art keywords
nucleic acids
extension product
basis
nucleotide differences
extension
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2078132A
Other languages
French (fr)
Other versions
CA2078132C (en
Inventor
Kenneth James Livak
Jan Antoni Rafalski
Scott Valray Tingey
John Gregory Kelly Williams
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23963743&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2078132(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Individual filed Critical Individual
Publication of CA2078132A1 publication Critical patent/CA2078132A1/en
Application granted granted Critical
Publication of CA2078132C publication Critical patent/CA2078132C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Abstract

A process is provided for distinguishing nucleic acids on the basis of nucleotide differences in random segments of the nucleic acid by performing a primer extension reaction on the nucleic acids and comparing the extension reaction products. The random nucleic acid segment can be amplified by first dissociating the extension product from the template and contacting the dissociated extension product with a primer under conditions such that an amplification extension product is synthesized using the dissociated extension product as a template. Differences in the extension product are useful as markers in constructing genetic maps and as markers to distinguish or identify individuals.
CA002078132A 1990-03-14 1991-02-12 A process for distinguishing nucleic acids on the basis of nucleotide differences Expired - Lifetime CA2078132C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/494,258 1990-03-14
US07/494,258 US5126239A (en) 1990-03-14 1990-03-14 Process for detecting polymorphisms on the basis of nucleotide differences
PCT/US1991/000841 WO1991014001A2 (en) 1990-03-14 1991-02-12 A process for distinguishing nucleic acids on the basis of nucleotide differences

Publications (2)

Publication Number Publication Date
CA2078132A1 true CA2078132A1 (en) 1991-09-15
CA2078132C CA2078132C (en) 1999-08-31

Family

ID=23963743

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002078132A Expired - Lifetime CA2078132C (en) 1990-03-14 1991-02-12 A process for distinguishing nucleic acids on the basis of nucleotide differences

Country Status (17)

Country Link
US (1) US5126239A (en)
EP (1) EP0520039B1 (en)
JP (1) JP2818486B2 (en)
AT (1) ATE115634T1 (en)
AU (1) AU639565B2 (en)
BR (1) BR9106151A (en)
CA (1) CA2078132C (en)
DE (1) DE69105959T2 (en)
DK (1) DK0520039T3 (en)
ES (1) ES2065684T3 (en)
FI (1) FI924049A (en)
IL (1) IL97503A (en)
NO (1) NO304655B1 (en)
NZ (1) NZ237396A (en)
PL (1) PL166895B1 (en)
WO (1) WO1991014001A2 (en)
ZA (1) ZA911891B (en)

Families Citing this family (101)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5595870A (en) * 1989-02-17 1997-01-21 The Trustees Of Columbia University In The City Of New York Identifying nucleic acids by restriction digestion and hybridization with random or pseudorandom oligonucleotides
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
JPH06500471A (en) * 1990-08-24 1994-01-20 ザ・ユニバーシティ・オブ・テネシー・リサーチ・コーポレーション DNA amplification fingerprinting
US6074818A (en) * 1990-08-24 2000-06-13 The University Of Tennessee Research Corporation Fingerprinting of nucleic acids, products and methods
WO1992004458A1 (en) * 1990-09-11 1992-03-19 The Brookdale Hospital Medical Center Drug resistance screening method
WO1992007095A1 (en) * 1990-10-15 1992-04-30 Stratagene Arbitrarily primed polymerase chain reaction method for fingerprinting genomes
US5635354A (en) * 1991-01-09 1997-06-03 Institut National De La Sante Et De La Recherche Medicale (Inserm) Method for describing the repertoires of antibodies (Ab) and of T-cell receptors (TcR) of an individual's immune system
US5401630A (en) * 1991-05-03 1995-03-28 The United States Of America As Represented By The Secretary Of Agriculture Species-specific DNA-DNA hybridization probe prepared using chromosome size DNA
EP0543484B1 (en) * 1991-08-30 2001-01-31 Research Development Corporation of Japan A method of DNA amplification
US20100267023A1 (en) 1992-09-24 2010-10-21 Keygene N.V. Selective restriction fragment amplification: fingerprinting
US7026115B1 (en) 1991-09-24 2006-04-11 Keygene N.V. Selective restriction fragment amplification: fingerprinting
EP0969102B1 (en) * 1991-09-24 2007-12-26 Keygene N.V. Primers, kits and sets of restriction fragments used in selective restriction fragment amplification
WO1993024655A1 (en) * 1992-05-27 1993-12-09 Amersham International Plc Rna analysis
US5340728A (en) * 1992-12-09 1994-08-23 E. I. Du Pont De Nemours And Company Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction
US5962221A (en) * 1993-01-19 1999-10-05 Univ Tennessee Res Corp Oligonucleotide constructs and methods for the generation of sequence signatures from nucleic acids
NZ263779A (en) * 1993-04-16 1997-03-24 F B Investments Pty Ltd Former Method for random amplification of dna
WO1995019697A1 (en) * 1994-01-21 1995-07-27 North Carolina State University Methods for within family selection in woody perennials using genetic markers
WO1995021269A1 (en) * 1994-02-04 1995-08-10 Perlin Mark K Method and apparatus for analyzing genetic material
US6037127A (en) * 1994-03-31 2000-03-14 E. I. Du Pont De Nemours And Company Method for detection of non-denatured nucleic acid fragments
US5831065A (en) * 1994-04-04 1998-11-03 Lynx Therapeutics, Inc. Kits for DNA sequencing by stepwise ligation and cleavage
US5552278A (en) * 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5714330A (en) * 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
US5547861A (en) * 1994-04-18 1996-08-20 Becton, Dickinson And Company Detection of nucleic acid amplification
US5585238A (en) * 1994-04-25 1996-12-17 Ciba-Geigy Corporation Detection of fungal pathogens using the polymerase chain reaction
US5660981A (en) * 1994-06-06 1997-08-26 E. I. Du Pont De Nemours And Company Selection of diagnostic genetic markers in microorganisms and use of a specific marker for detection of salmonella
US6013445A (en) * 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
USRE43097E1 (en) 1994-10-13 2012-01-10 Illumina, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
EP0804618B1 (en) * 1994-11-28 1999-01-27 E.I. Du Pont De Nemours And Company Compound microsatellite primers for the detection of genetic polymorphisms
ES2097701B1 (en) * 1995-02-09 1997-11-16 Consejo Superior Investigacion PROCEDURE FOR OBTAINING SPECIFIC STYLE OR SPECIES PROBES.
JPH09149799A (en) * 1995-11-30 1997-06-10 Hitachi Ltd Analysis or detection of nucleic acid and analyser or inspection device of nucleic acid
US5670325A (en) * 1996-08-14 1997-09-23 Exact Laboratories, Inc. Method for the detection of clonal populations of transformed cells in a genomically heterogeneous cellular sample
US5741650A (en) * 1996-01-30 1998-04-21 Exact Laboratories, Inc. Methods for detecting colon cancer from stool samples
US5747257A (en) * 1996-02-29 1998-05-05 E. I. Du Pont De Nemours And Company Genetic markers and methods for the detection of escherichia coli serotype-0157:H7
DE69737883T2 (en) 1996-04-25 2008-03-06 Bioarray Solutions Ltd. LIGHT-REGULATED, ELECTROKINETIC COMPOSITION OF PARTICLES TO SURFACES
US5811239A (en) * 1996-05-13 1998-09-22 Frayne Consultants Method for single base-pair DNA sequence variation detection
US6203993B1 (en) 1996-08-14 2001-03-20 Exact Science Corp. Methods for the detection of nucleic acids
US5952178A (en) * 1996-08-14 1999-09-14 Exact Laboratories Methods for disease diagnosis from stool samples
US6146828A (en) * 1996-08-14 2000-11-14 Exact Laboratories, Inc. Methods for detecting differences in RNA expression levels and uses therefor
US6100029A (en) * 1996-08-14 2000-08-08 Exact Laboratories, Inc. Methods for the detection of chromosomal aberrations
US6020137A (en) * 1996-08-14 2000-02-01 Exact Laboratories, Inc. Methods for the detection of loss of heterozygosity
US5928870A (en) * 1997-06-16 1999-07-27 Exact Laboratories, Inc. Methods for the detection of loss of heterozygosity
US6300077B1 (en) 1996-08-14 2001-10-09 Exact Sciences Corporation Methods for the detection of nucleic acids
US5814453A (en) * 1996-10-15 1998-09-29 Novartis Finance Corporation Detection of fungal pathogens using the polymerase chain reaction
US5800997A (en) * 1996-11-01 1998-09-01 Novartis Finance Corporation Detection of maize fungal pathogens using the polymerase chain reaction
US5922538A (en) * 1996-11-08 1999-07-13 E.I. Du Pont De Nemours And Company Genetic markers and methods for the detection of Listeria monocytogenes and Listeria spp
US6268136B1 (en) 1997-06-16 2001-07-31 Exact Science Corporation Methods for stool sample preparation
US6406857B1 (en) 1997-06-16 2002-06-18 Exact Sciences Corporation Methods for stool sample preparation
US5827695A (en) * 1997-08-04 1998-10-27 Novartis Finance Corporation Detection of wheat fungal pathogens using the polymerase chain reaction
US6173525B1 (en) 1997-09-19 2001-01-16 Cantharellus Ab Chanterelle mycelium
US20020064792A1 (en) * 1997-11-13 2002-05-30 Lincoln Stephen E. Database for storage and analysis of full-length sequences
JP2002501760A (en) * 1998-02-02 2002-01-22 アマーシャム・ファルマシア・バイオテック・アクチボラグ Nucleic acid analysis method
US7078224B1 (en) 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
EP1614475B1 (en) 1998-05-01 2007-05-30 Gen-Probe Incorporated Device for agitating the fluid contents of a container
US8337753B2 (en) * 1998-05-01 2012-12-25 Gen-Probe Incorporated Temperature-controlled incubator having a receptacle mixing mechanism
WO2000017399A2 (en) 1998-09-18 2000-03-30 The Board Of Regents Of The University Of Nebraska High resolution genome scanning
US6703228B1 (en) 1998-09-25 2004-03-09 Massachusetts Institute Of Technology Methods and products related to genotyping and DNA analysis
EP1001037A3 (en) * 1998-09-28 2003-10-01 Whitehead Institute For Biomedical Research Pre-selection and isolation of single nucleotide polymorphisms
JP2002531130A (en) * 1998-12-08 2002-09-24 チルドレンズ ホスピタル アンド レジオナル メディカル センター Escherichia coli O157: a polymorphic locus that distinguishes H7 from other strains
US6503718B2 (en) 1999-01-10 2003-01-07 Exact Sciences Corporation Methods for detecting mutations using primer extension for detecting disease
US6280947B1 (en) 1999-08-11 2001-08-28 Exact Sciences Corporation Methods for detecting nucleotide insertion or deletion using primer extension
DE60028732T2 (en) 1999-02-25 2007-06-14 Exact Sciences Corp., Maynard METHOD OF STABILIZING DNA INTEGRITY
FR2792000B1 (en) * 1999-04-08 2003-04-11 Centre Nat Rech Scient METHOD FOR MAPPING A DNA MOLECULE COMPRISING AN AD INFINITUM AMPLIFICATION STEP
ATE331811T1 (en) 1999-04-09 2006-07-15 Exact Sciences Corp METHOD FOR DETECTING NUCLEIC ACIDS INdicative of CANCER
US6586177B1 (en) * 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6919174B1 (en) * 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
CA2394921A1 (en) * 1999-12-07 2001-06-14 Anthony P. Shuber Supracolonic aerodigestive neoplasm detection
US6242191B1 (en) * 1999-12-30 2001-06-05 The Ohio State University Research Foundation Methods for assessing the beef characteristics of live cattle
NZ521626A (en) 2000-03-29 2005-09-30 Cambia Methods for genotyping by hybridization analysis
US9709559B2 (en) 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
JP4744778B2 (en) 2000-06-21 2011-08-10 バイオアレイ ソルーションズ リミテッド Method for analyzing multiple analyte molecules using a specific random particle array
US7695901B2 (en) * 2000-07-26 2010-04-13 North Carolina State University Identification of poinsettia cultivars
EP1207209A3 (en) * 2000-11-09 2002-09-11 Agilent Technologies, Inc. (a Delaware corporation) Methods using arrays for detection of single nucleotide polymorphisms
US6428964B1 (en) 2001-03-15 2002-08-06 Exact Sciences Corporation Method for alteration detection
US7262063B2 (en) 2001-06-21 2007-08-28 Bio Array Solutions, Ltd. Directed assembly of functional heterostructures
JP4377689B2 (en) 2001-10-15 2009-12-02 バイオアレイ ソリューションズ リミテッド Combined analysis of polymorphic loci with simultaneous interrogation and enzyme-mediated detection
US20030166190A1 (en) * 2001-12-10 2003-09-04 Wright David A. Nucleic acids related to plant retroelements
AU2003213107A1 (en) * 2002-02-15 2003-09-09 Exact Sciences Corporation Methods for analysis of molecular events
US7157228B2 (en) * 2002-09-09 2007-01-02 Bioarray Solutions Ltd. Genetic analysis and authentication
AU2003298655A1 (en) 2002-11-15 2004-06-15 Bioarray Solutions, Ltd. Analysis, secure access to, and transmission of array images
US20060068036A1 (en) 2002-12-31 2006-03-30 National Yang-Ming University Extract of Dioscorea sp. and the medical uses thereof
US20040259101A1 (en) * 2003-06-20 2004-12-23 Shuber Anthony P. Methods for disease screening
WO2005029705A2 (en) 2003-09-18 2005-03-31 Bioarray Solutions, Ltd. Number coding for identification of subtypes of coded types of solid phase carriers
NZ546072A (en) 2003-09-22 2009-08-28 Bioarray Solutions Ltd Surface immobilized polyelectrolyte with multiple functional groups capable of covalently bonding to biomolecules
WO2005042763A2 (en) 2003-10-28 2005-05-12 Bioarray Solutions Ltd. Optimization of gene expression analysis using immobilized capture probes
EP1694859B1 (en) 2003-10-29 2015-01-07 Bioarray Solutions Ltd Multiplexed nucleic acid analysis by fragmentation of double-stranded dna
WO2005111244A2 (en) * 2004-05-10 2005-11-24 Exact Sciences Corporation Methods for detecting a mutant nucleic acid
WO2005113769A1 (en) * 2004-05-14 2005-12-01 Exact Sciences Corporation Method for stabilizing biological samples for nucleic acid analysis
US7848889B2 (en) 2004-08-02 2010-12-07 Bioarray Solutions, Ltd. Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification
EP1623996A1 (en) * 2004-08-06 2006-02-08 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Improved method of selecting a desired protein from a library
US7981607B2 (en) * 2004-08-27 2011-07-19 Esoterix Genetic Laboratories LLC Method for detecting recombinant event
WO2006047787A2 (en) 2004-10-27 2006-05-04 Exact Sciences Corporation Method for monitoring disease progression or recurrence
US9777314B2 (en) * 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
US8486629B2 (en) 2005-06-01 2013-07-16 Bioarray Solutions, Ltd. Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation
WO2007005613A2 (en) * 2005-07-01 2007-01-11 Promega Corporation Network of buoyant particles for biomolecule purification
EP1963526A4 (en) 2005-12-09 2009-11-18 Promega Corp Nucleic acid purification with a binding matrix
US20110086343A1 (en) * 2006-03-10 2011-04-14 Hemant Jyotiswarup Purohit Method for the screening of bacterial isolates
US8481267B2 (en) 2009-08-21 2013-07-09 E. I. Du Pont De Nemours And Company Genetic fingerprinting and identification method
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US8222397B2 (en) * 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
AU2012287207A1 (en) 2011-07-25 2013-11-28 E. I. Du Pont De Nemours And Company Method to amplify nucleic acids to generate fluorescence labeled fragments of conserved and arbitrary products

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4851331A (en) * 1986-05-16 1989-07-25 Allied Corporation Method and kit for polynucleotide assay including primer-dependant DNA polymerase
EP0317239A3 (en) * 1987-11-13 1990-01-17 Native Plants Incorporated Method and device for improved restriction fragment length polymorphism analysis
AU632760B2 (en) * 1988-07-26 1993-01-14 Genelabs Technologies, Inc. Rna and dna amplification techniques
US4879214A (en) * 1988-11-15 1989-11-07 E. I. Du Pont De Nemours And Company Differentiation of nucleic acid segments on the basis of nucleotide differences
IE66572B1 (en) * 1989-02-13 1996-01-24 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers

Also Published As

Publication number Publication date
AU639565B2 (en) 1993-07-29
DK0520039T3 (en) 1995-02-20
NZ237396A (en) 1993-08-26
NO304655B1 (en) 1999-01-25
CA2078132C (en) 1999-08-31
WO1991014001A3 (en) 1991-10-31
PL293188A1 (en) 1992-11-02
BR9106151A (en) 1993-03-09
FI924049A0 (en) 1992-09-10
US5126239A (en) 1992-06-30
DE69105959D1 (en) 1995-01-26
NO923544D0 (en) 1992-09-11
IL97503A (en) 1995-05-26
JPH05505528A (en) 1993-08-19
WO1991014001A2 (en) 1991-09-19
EP0520039A1 (en) 1992-12-30
AU7782091A (en) 1991-10-10
JP2818486B2 (en) 1998-10-30
PL166895B1 (en) 1995-06-30
ZA911891B (en) 1992-11-25
ES2065684T3 (en) 1995-02-16
FI924049A (en) 1992-09-10
EP0520039B1 (en) 1994-12-14
IL97503A0 (en) 1992-06-21
NO923544L (en) 1992-09-11
DE69105959T2 (en) 1995-05-18
ATE115634T1 (en) 1994-12-15

Similar Documents

Publication Publication Date Title
CA2078132A1 (en) A process for distinguishing nucleic acids on the basis of nucleotide differences
CA2087724A1 (en) Reduction of non-specific amplification during in vitro nucleic acid amplification using modified nucleic acid bases
US4683202B1 (en)
NO854334L (en) TRANSFORMATION OF YEAR SPECIES OF THE PICHIA genus.
CA2205353A1 (en) Continuous amplification reaction
CA2172722A1 (en) Methods and compositions for efficient nucleic acid sequencing
CA2005589A1 (en) Self-sustained, sequence replication system
ATE49977T1 (en) PRODUCTION OF DNA ON A LARGE SCALE.
GB9210176D0 (en) Chemical method
EP2374890A3 (en) Fen-1 endonucleases, mixtures and cleavage methods
CA2171110A1 (en) Detection of amplified or deleted chromosomal regions
CA2209080A1 (en) Fluorometric assay for detecting nucleic acid cleavage
ATE106948T1 (en) METHODS FOR AMPLIFICATION OF NUCLEIC ACIDS.
EP0269445A3 (en) Process for detecting HTLVI and HTLVII viruses by hybridization
CA2018562A1 (en) Process for the enzymatic cleavage of vinyl 2-aryl-propionates
EP0304495A4 (en) Process for selectively monohydroesterifying diolefin.
WO1993022459A3 (en) Oligonucleotides and nucleic acids for detection of ureaplasma urealyticum
ES2018917A6 (en) Replicative rna-based amplification/detection systems.
EP1302547A3 (en) A method of detecting and discriminating between nucleic acid sequences

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed
MKEC Expiry (correction)

Effective date: 20121202