CA2100268A1 - Method for culturing and transforming human stem cell-containing compositions - Google Patents

Method for culturing and transforming human stem cell-containing compositions

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Publication number
CA2100268A1
CA2100268A1 CA002100268A CA2100268A CA2100268A1 CA 2100268 A1 CA2100268 A1 CA 2100268A1 CA 002100268 A CA002100268 A CA 002100268A CA 2100268 A CA2100268 A CA 2100268A CA 2100268 A1 CA2100268 A1 CA 2100268A1
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CA
Canada
Prior art keywords
human
culture
culturing
human stem
stem cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002100268A
Other languages
French (fr)
Other versions
CA2100268C (en
Inventor
Stephen G. Emerson
Michael F. Clarke
Bernhard O. Palsson
Richard M. Schwartz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Michigan
Original Assignee
Stephen G. Emerson
Michael F. Clarke
Bernhard O. Palsson
Richard M. Schwartz
The Regents Of The University Of Michigan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stephen G. Emerson, Michael F. Clarke, Bernhard O. Palsson, Richard M. Schwartz, The Regents Of The University Of Michigan filed Critical Stephen G. Emerson
Publication of CA2100268A1 publication Critical patent/CA2100268A1/en
Application granted granted Critical
Publication of CA2100268C publication Critical patent/CA2100268C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Abstract

Methods, including culture media conditions and reactors, which provide for ex vivo human stem cell division and stable genetic transformation and/or the optimization of human hematopoietic progenitor cell cultures and/or increasing the metabolism or GM-CSF secretion or IL-6 secretion of human stromal cells are disclosed.
The methods rely on culturing human stem cells and/or human hemapoietic progenitor cells and/or human stromal cells in a liquid culture medium which is replaced, preferably perfused, either continuously or periodically, at a rate of 1 ml of medium per ml of culture per about 24 to about 48 hour period, and removing metabolic products and replenishing depleted nutrients while maintaining the culture under physiologically acceptable conditions. Optionally growth factors are added to the culture medium.
CA002100268A 1990-12-17 1991-12-17 Method for culturing and transforming human stem cell-containing compositions Expired - Lifetime CA2100268C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US62834390A 1990-12-17 1990-12-17
US628,343 1990-12-17
US73702491A 1991-07-29 1991-07-29
US737,024 1991-07-29
US740,590 1991-08-05
US07/740,590 US5399493A (en) 1989-06-15 1991-08-05 Methods and compositions for the optimization of human hematopoietic progenitor cell cultures
PCT/US1991/009173 WO1992011355A1 (en) 1990-12-17 1991-12-17 Method for culturing and transforming human stem cell-containing compositions

Publications (2)

Publication Number Publication Date
CA2100268A1 true CA2100268A1 (en) 1992-06-18
CA2100268C CA2100268C (en) 2008-12-02

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CA002100268A Expired - Lifetime CA2100268C (en) 1990-12-17 1991-12-17 Method for culturing and transforming human stem cell-containing compositions

Country Status (9)

Country Link
US (2) US5399493A (en)
EP (2) EP1473360A3 (en)
JP (5) JPH06505151A (en)
KR (1) KR100225307B1 (en)
AT (1) ATE295412T1 (en)
AU (1) AU687674B2 (en)
CA (1) CA2100268C (en)
DE (1) DE69133459T2 (en)
WO (1) WO1992011355A1 (en)

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US5635386A (en) * 1989-06-15 1997-06-03 The Regents Of The University Of Michigan Methods for regulating the specific lineages of cells produced in a human hematopoietic cell culture
US5399493A (en) * 1989-06-15 1995-03-21 The Regents Of The University Of Michigan Methods and compositions for the optimization of human hematopoietic progenitor cell cultures
US5605822A (en) * 1989-06-15 1997-02-25 The Regents Of The University Of Michigan Methods, compositions and devices for growing human hematopoietic cells
US5635387A (en) * 1990-04-23 1997-06-03 Cellpro, Inc. Methods and device for culturing human hematopoietic cells and their precursors
US5612211A (en) * 1990-06-08 1997-03-18 New York University Stimulation, production and culturing of hematopoietic progenitor cells by fibroblast growth factors
EP0535148B1 (en) * 1990-06-08 1998-03-18 New York University Stimulation of bone marrow stromal and progenitor cells
US20030125519A1 (en) 1990-08-27 2003-07-03 Peter Besmer Ligand for the c-kit receptor and methods of use thereof
US5744361A (en) * 1991-04-09 1998-04-28 Indiana University Expansion of human hematopoietic progenitor cells in a liquid medium
US5199942A (en) * 1991-06-07 1993-04-06 Immunex Corporation Method for improving autologous transplantation
NL9101680A (en) * 1991-10-04 1993-05-03 Tno METHOD FOR GENETICALLY MODIFYING PRIMATE BONE MARROW CELLS AND USE CELLS PRODUCING RECOMBINANT RETROVIRAL VECTORS.
DE69332374T2 (en) * 1992-03-04 2003-06-12 Univ Michigan Ann Arbor METHOD, COMPOSITIONS AND DEVICES FOR THE PRESERVATION AND BREEDING OF HUMAN STEM CELLS AND / OR HEMATOPOETIC CELLS
WO1993018648A1 (en) * 1992-03-23 1993-09-30 Baxter International Inc. In vitro-derived human neutrophil precursor cells
US6001803A (en) * 1992-04-23 1999-12-14 Sloan-Kettering Institute For Cancer Research Composition of c-kit ligand, GM-CSF, and TNF-α and method of use
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CA2100268C (en) 2008-12-02
AU9175091A (en) 1992-07-22
WO1992011355A1 (en) 1992-07-09

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