CA2104780A1 - Lipid fractionation - Google Patents

Lipid fractionation

Info

Publication number
CA2104780A1
CA2104780A1 CA002104780A CA2104780A CA2104780A1 CA 2104780 A1 CA2104780 A1 CA 2104780A1 CA 002104780 A CA002104780 A CA 002104780A CA 2104780 A CA2104780 A CA 2104780A CA 2104780 A1 CA2104780 A1 CA 2104780A1
Authority
CA
Canada
Prior art keywords
class
lipoprotein
sample
lipoproteins
precipitated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002104780A
Other languages
French (fr)
Other versions
CA2104780C (en
Inventor
Joseph F. Lawlor
Joseph D. Musto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Reference Diagnostics Inc
Original Assignee
Joseph F. Lawlor
Joseph D. Musto
Reference Diagnostics, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Joseph F. Lawlor, Joseph D. Musto, Reference Diagnostics, Inc. filed Critical Joseph F. Lawlor
Publication of CA2104780A1 publication Critical patent/CA2104780A1/en
Application granted granted Critical
Publication of CA2104780C publication Critical patent/CA2104780C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/04—Endocrine or metabolic disorders
    • G01N2800/044—Hyperlipemia or hypolipemia, e.g. dyslipidaemia, obesity
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

The method separates a first class of lipoprotein from a second class of lipoprotein in a sample and analyzes the first class of lipoprotein. The second class of lipoprotein is first precipitated. The sample is then contacted with magnetically responsive particles. The sample is then placed in a magnetic field until the magnetically responsive particles have sedimented, thereby caus-ing the precipitated second class of lipoproteins to sediment. The first class of lipoproteins is left in the supernatant of the sample for subsequent analysis.
CA002104780A 1991-03-20 1992-03-17 Lipid fractionation Expired - Fee Related CA2104780C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/677,734 US5242833A (en) 1991-03-20 1991-03-20 Lipid fractionation
US677,734 1991-03-20
PCT/US1992/002170 WO1992016300A1 (en) 1991-03-20 1992-03-17 Lipid fractionation

Publications (2)

Publication Number Publication Date
CA2104780A1 true CA2104780A1 (en) 1992-09-21
CA2104780C CA2104780C (en) 2002-05-28

Family

ID=24719912

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002104780A Expired - Fee Related CA2104780C (en) 1991-03-20 1992-03-17 Lipid fractionation

Country Status (5)

Country Link
US (3) US5242833A (en)
EP (1) EP0576562A4 (en)
JP (1) JP2913608B2 (en)
CA (1) CA2104780C (en)
WO (1) WO1992016300A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5758090A (en) * 1995-09-22 1998-05-26 Airnet Communications, Inc. Frequency reuse planning for CDMA cellular communication system by grouping of available carrier frequencies and power control based on the distance from base station

Families Citing this family (24)

* Cited by examiner, † Cited by third party
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US5523231A (en) * 1990-02-13 1996-06-04 Amersham International Plc Method to isolate macromolecules using magnetically attractable beads which do not specifically bind the macromolecules
US5242833A (en) * 1991-03-20 1993-09-07 Reference Diagnostics, Inc. Lipid fractionation
DE4223355A1 (en) * 1992-07-16 1994-01-20 Boehringer Mannheim Gmbh Method and reagent for the specific determination of LDL in serum samples
US5401466A (en) * 1993-06-01 1995-03-28 Miles Inc. Device for the direct measurement of low density lipoprotein cholesterol
US6368866B1 (en) 1998-08-03 2002-04-09 Reference Diagnostics, Inc. Rapid separation assay for total iron binding capacity
KR20020015246A (en) * 2000-08-21 2002-02-27 김주호 The diagnostic method and device of HDL-cholesterol and LDL-cholesterol simultaneously using magnetic bead
AU2003226075B2 (en) * 2002-04-12 2008-09-25 Instrumentation Laboratory Company Immunoassay probe
US20040157219A1 (en) * 2003-02-06 2004-08-12 Jianrong Lou Chemical treatment of biological samples for nucleic acid extraction and kits therefor
US7601491B2 (en) * 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
US20050074828A1 (en) * 2003-07-16 2005-04-07 Dimagno Theodore John Uae of lipase for high-density lipoprotein cholesterol detection
US20050239091A1 (en) * 2004-04-23 2005-10-27 Collis Matthew P Extraction of nucleic acids using small diameter magnetically-responsive particles
US8211386B2 (en) 2004-06-08 2012-07-03 Biokit, S.A. Tapered cuvette and method of collecting magnetic particles
US20060024776A1 (en) * 2004-08-02 2006-02-02 Mcmillian Ray Magnetic particle capture of whole intact organisms from clinical samples
JP5053089B2 (en) * 2004-08-03 2012-10-17 ベクトン・ディキンソン・アンド・カンパニー Use of magnetic materials for direct isolation of compounds and fractionation of multicomponent samples
WO2006017427A1 (en) * 2004-08-03 2006-02-16 Becton, Dickinson And Company Use of magnetic material to fractionate samples
US8084275B2 (en) * 2005-02-08 2011-12-27 Fujifilm Corporation Magnetic composite body, production method thereof, method for removing substance with mannose on its surface, and method for concentrating substance with mannose on its surface
JP2007040978A (en) * 2005-06-30 2007-02-15 Fujifilm Corp Method for separating objective element using magnetic nanoparticles
US8247235B2 (en) 2007-06-08 2012-08-21 Quest Diagnostics Investments Incorporated Lipoprotein analysis by differential charged-particle mobility
EP2171098B1 (en) * 2007-06-29 2018-03-28 Becton, Dickinson and Company Methods for extraction and purification of components of biological samples
US8785146B2 (en) * 2008-04-01 2014-07-22 Beijing Strong Biotechnologies, Inc. Method for quantitative measurements of HDL-C and LDL-C
US9354200B1 (en) 2008-08-07 2016-05-31 Quest Diagnostics Investments Incorporated Detection apparatus for differential-charged particle mobility analyzer
US9250211B2 (en) 2010-12-30 2016-02-02 Quest Diagnostics Investments Incorporated Magnetic separation of lipoproteins using dextran sulfate
US20150260631A1 (en) * 2014-03-17 2015-09-17 Health Diagnostic Laboratory, Inc. System and method for assessing quanitites or sizes of lipoprotein particles from lipoprotein particle compositions
ES2724537T3 (en) * 2013-12-03 2019-09-11 Immunodiagnostic Systems Ltd Procedure for quantifying an analyte, and an automatic analytical device configured to implement said procedure

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4210557A (en) * 1978-12-15 1980-07-01 Beckman Instruments, Inc. Non-high density lipoprotein precipitant
JPS58127168A (en) * 1982-01-25 1983-07-28 Eiken Kagaku Kk Composition for determining high specific gravity lipo protein cholesterol
SE8201972L (en) * 1982-03-29 1983-09-30 Gambro Lundia Ab MAGNETIC PORTABLE CRYSTALLIZED CARBOHYDRATED SPHERES OR PARTICLES TO BE USED TOGETHER WITH BIODOUS PREPARING MATERIALS
US4861705A (en) * 1983-01-31 1989-08-29 Yeda Research And Development Company, Ltd. Method for removing components of biological fluids
US4628037A (en) * 1983-05-12 1986-12-09 Advanced Magnetics, Inc. Binding assays employing magnetic particles
US4554088A (en) * 1983-05-12 1985-11-19 Advanced Magnetics Inc. Magnetic particles for use in separations
US4920061A (en) * 1984-03-02 1990-04-24 The University Of Texas System Biological magnetic colloids
US4795698A (en) * 1985-10-04 1989-01-03 Immunicon Corporation Magnetic-polymer particles
US5076950A (en) * 1985-12-20 1991-12-31 Syntex (U.S.A.) Inc. Magnetic composition for particle separation
CA1304006C (en) * 1985-12-20 1992-06-23 Edwin F. Ullman Particle separation method
US4661408A (en) * 1986-03-18 1987-04-28 E.I. Du Pont De Nemours And Company Coated chromium dioxide particles
AU7399487A (en) * 1986-05-22 1987-12-22 Unilever Plc Solid phase immunoassay method
GB8620893D0 (en) * 1986-08-29 1986-10-08 Unilever Plc Assays
EP0317286B1 (en) * 1987-11-16 1994-07-13 Amoco Corporation Magnetic separation device and methods for use in heterogeneous assays
DE68920778T2 (en) * 1988-05-24 1995-05-18 Anagen Uk Ltd Magnetically attractable particles and manufacturing processes.
US5242833A (en) * 1991-03-20 1993-09-07 Reference Diagnostics, Inc. Lipid fractionation

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5758090A (en) * 1995-09-22 1998-05-26 Airnet Communications, Inc. Frequency reuse planning for CDMA cellular communication system by grouping of available carrier frequencies and power control based on the distance from base station

Also Published As

Publication number Publication date
US5595913A (en) 1997-01-21
JPH06507605A (en) 1994-09-01
JP2913608B2 (en) 1999-06-28
US5242833A (en) 1993-09-07
EP0576562A1 (en) 1994-01-05
AU660015B2 (en) 1995-06-08
EP0576562A4 (en) 1994-12-21
WO1992016300A1 (en) 1992-10-01
CA2104780C (en) 2002-05-28
AU1572392A (en) 1992-10-21
US5422279A (en) 1995-06-06

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Legal Events

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EEER Examination request
MKLA Lapsed