CA2108292A1 - Process for preparing hepatitis a (hav) antigens and vaccines - Google Patents

Process for preparing hepatitis a (hav) antigens and vaccines

Info

Publication number
CA2108292A1
CA2108292A1 CA002108292A CA2108292A CA2108292A1 CA 2108292 A1 CA2108292 A1 CA 2108292A1 CA 002108292 A CA002108292 A CA 002108292A CA 2108292 A CA2108292 A CA 2108292A CA 2108292 A1 CA2108292 A1 CA 2108292A1
Authority
CA
Canada
Prior art keywords
hav
vaccines
antigens
carried out
preparing hepatitis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002108292A
Other languages
French (fr)
Other versions
CA2108292C (en
Inventor
Bernard Fanget
Alain Francon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanofi Pasteur SA
Original Assignee
Bernard Fanget
Alain Francon
Pasteur Merieux Serums Et Vaccins
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bernard Fanget, Alain Francon, Pasteur Merieux Serums Et Vaccins filed Critical Bernard Fanget
Publication of CA2108292A1 publication Critical patent/CA2108292A1/en
Application granted granted Critical
Publication of CA2108292C publication Critical patent/CA2108292C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32411—Hepatovirus, i.e. hepatitis A virus
    • C12N2770/32422—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32411—Hepatovirus, i.e. hepatitis A virus
    • C12N2770/32434—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/948—Microorganisms using viruses or cell lines

Abstract

Process for preparing hepatitis A (HAV) antigens and vaccines.
The HAV virus is multiplied on competent cells, the infected cells are lysed, the supernatant is recovered and the purification is carried out by a chromatographic procedure on an anion-exchange support and a gel filtration procedure, the purification procedures being carried out in the presence of a detergent, and the chromatographic procedure being carried out under conditions which retain the virions or viral capsids, which are then eluted.
CA002108292A 1992-10-14 1993-10-13 Process for preparing hepatitis a (hav) antigens and vaccines Expired - Lifetime CA2108292C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9212285A FR2696748B1 (en) 1992-10-14 1992-10-14 Process for the preparation of hepatitis A antigens and vaccines (HAV).
FR9212285 1992-10-14

Publications (2)

Publication Number Publication Date
CA2108292A1 true CA2108292A1 (en) 1994-04-15
CA2108292C CA2108292C (en) 2005-04-12

Family

ID=9434534

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002108292A Expired - Lifetime CA2108292C (en) 1992-10-14 1993-10-13 Process for preparing hepatitis a (hav) antigens and vaccines

Country Status (10)

Country Link
US (1) US5731187A (en)
EP (1) EP0593339B1 (en)
JP (1) JP3724821B2 (en)
AT (1) ATE179428T1 (en)
CA (1) CA2108292C (en)
DE (1) DE69324645T2 (en)
DK (1) DK0593339T3 (en)
ES (1) ES2132204T3 (en)
FR (1) FR2696748B1 (en)
GR (1) GR3030636T3 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6908598A (en) * 1996-10-29 1998-05-22 Smithkline Beecham Biologicals (Sa) Purification of respiratory syncytial virus antigens
US6261823B1 (en) 1996-12-13 2001-07-17 Schering Corporation Methods for purifying viruses
US6544769B1 (en) 1996-12-13 2003-04-08 Schering Corporation Compostions comprising viruses and methods for concentrating virus preparations
ES2349046T3 (en) * 1996-12-13 2010-12-22 Schering Corporation METHODS TO PURIFY VIRUSES.
AU2003229159B2 (en) * 2002-04-30 2008-02-21 Oncolytics Biotech Inc. Improved viral purification methods
JPWO2006011580A1 (en) * 2004-07-27 2008-05-01 ジェノミディア株式会社 Purification method of virus envelope
US7901921B2 (en) * 2004-10-22 2011-03-08 Oncolytics Biotech Inc. Viral purification methods
TR201902201T4 (en) * 2015-07-23 2019-03-21 Grifols Sa METHODS FOR PURIFICATION OF AN IN VITRO GENERATED VIRUS AND ANALYSIS FOR VIRUS.
EP3695849A1 (en) * 2019-02-13 2020-08-19 Mediagnost Gesellschaft für Forschung und Herstellung von Diagnostika GmbH Method for extracting hepatitis a virus (hav) antigen from cell culture
KR102277089B1 (en) * 2019-12-19 2021-07-14 에스케이바이오사이언스(주) Method for Preparing Hepatitis A Virus and Hepatitis A Virus Prepared by the Method

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ225583A (en) * 1987-08-06 1991-07-26 Merck & Co Inc Process for purifying hepatitis a virions (hav)
JPH0761955B2 (en) * 1988-04-28 1995-07-05 国立予防衛生研究所長 Lyophilized hepatitis A vaccine
CA2047041A1 (en) * 1990-07-18 1992-01-19 John A. Lewis Process for purification of hepatitis-a virus capsids
IT1248075B (en) * 1991-06-18 1995-01-05 Sclavo Spa HEPATITIS A (HAV) VIRUS PURIFICATION PROCESS, PURIFIED VIRUS AND VACCINAL COMPOSITIONS THAT CONTAIN IT.

Also Published As

Publication number Publication date
DE69324645T2 (en) 1999-12-30
EP0593339B1 (en) 1999-04-28
JP3724821B2 (en) 2005-12-07
ATE179428T1 (en) 1999-05-15
DK0593339T3 (en) 1999-11-08
CA2108292C (en) 2005-04-12
ES2132204T3 (en) 1999-08-16
FR2696748B1 (en) 1994-12-30
GR3030636T3 (en) 1999-10-29
JPH06279317A (en) 1994-10-04
EP0593339A1 (en) 1994-04-20
FR2696748A1 (en) 1994-04-15
DE69324645D1 (en) 1999-06-02
US5731187A (en) 1998-03-24

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Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20131015