CA2123048C - Tetrazole derivatives of bile acids, processes for their preparation and the use of these compounds as medicaments - Google Patents

Abstract

Tetrazole-bile acid derivatives of the formula I

in which G1, G2 and X have the meanings given and processes for their preparation are described. The compounds have useful pharmacological properties and can therefore be used as medicaments.

Description

HOECHST ARTIENGESELLSCHAFT HOE 93/F 130 Dr.D/wo Description Tetrazole derivatives of bile acids, processes for their preparation and the use of these compounds as medica ments.
The invention relates to bile acid derivatives of the formula I
Gl-X-G2 I, processes for their preparation, pharmaceutical prepara-tions based on these compounds and the use of the bile acid derivatives as medicaments.
The compounds according to the invention have a high affinity for the specific bile acid transportation system of the small intestine and inhibit bile acid resorption in a concentration-dependent and competitive manner.
Bile acids have an important physiological function in lipolysis, for example as cofactors of pancreatic lipases and as natural detergents for solubilization of fats and fat-soluble vitamins. As the end product of cholesterol metabolism, they are synthesized in the liver, stored in the gallbladder and secreted from this by contraction into the small intestine, where they display their physiological action. The greatest proportion of bile acids secreted is recovered via the enterohepatic circu-lation. They return to the liver by the mesenterial veins of the small intestine and the portal vein system. Both active and passive transportation processes play a role in re-absorption in the intestine. A large proportion of the bile acids is reabsorbed at the end of the small intestine, the terminal ileum, by a specific Na'-depen-dent transportation system and return to the liver with the mesenterial venous blood via the portal vein, to be secreted again by the liver cells into the bile. Bile _~123~48 acids occur in the enterohepatic circulation both as free acids and in the form of glycine conjugates and taurine conjugates.
Non-absorbable, insoluble, basic, crosslinked polymers have been used for a long time for binding bile acids, and have been used therapeutically on the basis of these properties. Bile acid derivatives described in patent application EP-A-0 489 423 have a high affinity for the intestinal bile acid transportation system and therefore allow specific inhibition of the enterohepatic circula-tion. All diseases where inhibition of bile acid resorp-tion in the intestine, in particular in the small intes-tine, seems desirable are regarded as therapeutic sub-jects. For example, biligenic diarrhea following ileum resection, or increased blood cholesterol levels are treated in this manner. In the case of an increased blood cholesterol level, a reduction in this level can be achieved by intervention in the enterohepatic circu-lation. Reducing the bile acid pool in the enterohepatic circulation forces corresponding new synthesis of bile acids from cholesterol in the liver. The LDL cholesterol in the blood circulation is resorted to in order to meet the cholesterol requirement in the liver, the hepatic LDL
receptors being increasingly used. The acceleration in LDL catabolism achieved in this way has the effect of reducing the atherogenic cholesterol content in the blood.
Most natural bile acids have a terminal carboxyl group (carbon atom 24) in the side chain on the D ring of the steroid structure. The carboxyl group is in the free form or is conjugated with an amino acid.
The tetrazol-5-yl group is an isosteric group to the carboxyl function, i.e. it has similar steric, electronic and acid properties to the carboxyl function itself. It is known from medical chemistry that when the carboxyl group of certain active compounds is replaced by a 212304.8 tetrazol-5-yl radical, the affinity for enzymes and proteins is retained or increased and a better action potential can thus be achieved (Drugs of the Future 1992, 17 (7) 575 to 593) .
The object was to discover novel medicaments which are capable of reducing the atherogenic cholesterol content in the blood or of influencing the enterohepatic circu-lation in respect of increased secretion of bile acid and subsequent reduction in the cholesterol level.
This object is achieved by the tetrazole-bile acid derivatives according to the invention.
The invention therefore relates to tetrazole-bile acid derivatives of the formula I
Gl-X-G2 I
in which Gl is H, a bile acid radical or a modified bile acid radical which is modified on the hydroxyl functions and/or on the carboxyl group and G2 is a bile acid radical or a bile acid radical deri-vatized on the hydroxyl functions, which carries a tetrazolyl radical in the side chain, and X is a bridge group or a covalent bond, and in which Gl and G2 can be bonded via X as desired.
The compounds according to the invention have a high affinity for the specific bile acid transportation system of the small intestine and in.'hibit bile acid resorption in a concentration-dependent and competitive manner.
Furthermore, the compounds according to the invention are not themselves absorbed and therefore do not enter into the blood circulation. The enterohepatic circulation of bile acid can be interrupted very specifically and _ 2123048 efficiently by application of this action principle.
By using the compounds according to the invention it is possible to reduce the amount of bile acid in the entero-hepatic circulation, so that a reduction in the choles-terol level in the serum takes place. Avitaminoses are just as unlikely during use as an influence on the absorption of other medicaments or an adverse effect on the intestinal flora. The side-effects known to be caused by polymers (constipation, steatorrhea) furthermore are not observed, i.e. lipolysis is not adversely influenced.
Because of the high affinity for the specific bile acid transportation system of the small intestine, low daily doses are sufficient, so that acceptance of such medica-ments by the doctor and patient will be very high.
Preferred compounds of the formula I are those in which the linkage between the steroid structures of Gl and G2 is not via in each case the same rings of the steroid structure, and in which X is a covalent bond or a bridge group, G1 is H, a bile acid radical or a bile acid radical modified on the hydroxyl functions and/or on the carboxyl group and G2 is a bile acid radical or a bile acid radical deri vatized on the hydroxyl functions. which carries a tetrazolyl radical in the side chain.
Particularly preferred compounds of the formula I are those in which the linkage of G1 and G2 is via the ring D of Gl and the ring A of G2, and in which G1 is H or a radical of the formula II, R(2).R(3) II
i~R(4) R(1)0 H R(5) in which R(1) is H, an alkyl radical or alkenyl radical having up to 10 carbon atoms, which is branched or unbranched, a cycloalkyl radical having 3 to 8 carbon atoms, a benzyl radical, which is unsubstituted or mono- to trisubstituted by F, C1, Br, (C1-C~) -alkyl or (Cl-C,,) -alkoxy, a diphenylmethyl radical, which is unsubstituted or mono- to trisubstituted by F, Cl, Hr, (Cl-C,,)-alkyl or (C1-C,) -alkoxy, a triphenylmethyl radical, which is unsubstituted or mono- to trisubstituted by F, Cl, Hr, (Cl-C,,) -alkyl or (Cl-C,) -alkoxy, a Cl-C,,-alkoxymethyl or a tetrahydroxylamyl radical, or a radical I I II
- , p ~ S- or L-C-I II

in which L is H, an alkyl or alkenyl radical having up to 10 carbon atoms, which is branched or unbranched, a cycloalkyl radical having 3 to 8 carbon atoms, a phenyl radical, which is unsubstituted or mono- to trisubstituted by F, Cl, Hr, (Cl-C,,) -alkyl or (C1-C~)-alkoxy, or a benzyl radical, which is 21~3~4~

unsubstituted or mono- to trisubstituted by F, Cl, 8r, (C1-C,) -alkyl or (C1-C,,) -alkoxy, X is a single bond or a bridge member of the formula III

II II
-f-(N)=-A-N-C-(CH2)q-C-l,-N-(B)i- I I I
L(t) L(2) L(3) in which A is an alkylene chain, which is branched or un-branched and can optionally be interrupted by -O-, -S- or phenylene, the linkage to the phenylene ring being in the ortho-, mete- or pare-position and the chain comprising in total 2 to 12, preferably 2 to 6, chain members p, B is an alkylene chain, which is branched or un-branched and can optionally be interrupted by -O-, -S- or phenylene, the linkage to the phenylene ring being in the ortho-, mete- or pare-position and the chain comprising in total 2 to 12, preferably 2 to 6, chain members n, L(1), L(2) and L(3) are identical or different and have the meaning of L, and q is 0 to 5 r is 0 or l, s is 0 or 1 and t is 0 or l, R(2) to R(5) , where R(2) and R(3) or R(4) and R(5) in each case together are the oxygen of a carbonyl group. or individually and in each case indepen-dently of one another are H, -OL, -SL, -NHL, tetra-hydropyranyloxy or C1-Cs-alkoxymethoxy, in which L
has the abovementioned meaning, and 212~~~$
_~_ G2 is a radical of the formula IV
R(6) R(7) N~''' N H
Z
,.-.-N
N
IV
v in which Z is -(CHy)m N-(CH2~o-n where m is zero to 4, n is zero or 1 and o is zero to 4, V -0-, -N-, -CH2-, -CH2CH2-L
W is H or, if V is -CHz or -CHzCHs-, is also OH
and R(6) to R(9) have the meaning given under R(2) to R(5) .
Especially preferred compounds of the formula I are those in which Gl is H or a radical of the formula II
R(2).R(3) 0 II
H H
R(4) R(1)0 H R(5) W H R(9) 2123~~8 -8- _ in which k(1) is H, formyl, acetyl, benzoyl, methoxymethyl or tetrahydropyranyl, W H R(g) R(2) to R(5), where R(2) and R(3) or R(4) and R(5) in each case together are the oxygen of a carbonyl group, or individually and independently of one another are H, OH, O-formyl, O-acetyl, O-benzoyl, methoxymethoxy or tetrahydropyranyloxy, X is a covalent bond or one of the following bridge groups -N-(CHi)~- . -N-(CH=)3- . -N-(CHq)6- . -N-(CHz)Z-0-(CH=)Z- , H H H H

-H-( CHZ)a'H~ (CH2)2~ H-(CH2)2-G2 is a radical of the formula IV
V
R(6) R(7) N ~'' N H
~N
N
H
f~
s H H
R(8) !V

223048.
_ g _ in which V is -O-, -N-H
W is H, -~CH2~m H-(CHy~o-n where m is 1 to 3, n is zero or 1 and o is zero, 1 or 2, and R(6) to R(9) have the meaning given above under R(2) to R(5) .
The invention furthermore relates to a process for the preparation of compounds of the formula I, which com prises a) in the case where X = a single bond, reacting suit-able reactive forms of G1 and G2 with one another by processes which are known in principle, or b) in the case where X = a bridge group, reacting a) reactive forms of G1-X with G2 or ~B) reactive forms of G2-X with G1 with one another by processes which are known in principle.
a) X = a single bond The bile acids G1 are employed either in the free form or in protected form. After linkage with G2, which is likewise in the free or protected form, the protective groups are split off, if appropriate.
Suitable protective groups for the alcohol groups are expediently formyl, acetyl or tetrahydropyranyl.
The tetrazolyl function is either already present in G2 before the linking operation, or is introduced via a carboxyl group of G2 after the linking with Gl.

For example, bile acid preferentially reacts at position 3, but also at position 7, witr. activated forms of carboxylic acids, such as acid chlorides or mixed anhydrides, with addition of bases, such as trialkylamine or pyridine, or also NaOH, at room temperature in suitable solvents, such as tetra-hydrofuran, methylene chloride or ethyl acetate, or else dimethylformamide (DMF) or dimethoxyethane (DME). The various isomers can be separated, for example, by chromatography.
The reaction can be carried out selectively by using suitable protective groups.
Corresponding amino-bile acids can be converted into corresponding amides analogously. Here also, the reaction can be carried out either with protected or with free bile acids.
Other compounds according to the invention can be linked analogously by known standard processes.
b) X = a bridge member The processes described under a) are also used to carry out the linking of Gl-X with G2 or G1 with X-G2.
Here also, the bile acid portion is expediently employed in either protected or unprotected form.
A preferred preparation process comprises reacting reactive forms of G1 with reactive forms of X-G2. If appropriate, the linking operation is followed by splitting off protective groups and converting into a tetrazole derivative.
The preparation of tetrazolyl-bile acid units or tetra-zolyl-bile acids is described in the following equations.

21234.8 Equation 1 OH -HO H .. OHCO H ~~
V R - H, OH VI R = H, OCHO
CAN CAN
HO H ~' R'0 ~~
H
VII R = H, OH VIII R ~ H, OTHP, OMOM
R' = THP, MOM
i i ~N ~N
i -N H -N H
H 0 H .. R ~ p H ..
X R = H, OH IX R = H, OTHP, OMOM
R' = THP, MOM
THP = tetrahydropyranyl, MOM = methoxymethyl C-22-tetrazolyl-cholanic acid derivatives X are acces-sible by first shortening the side chain of a C-24-cholanic acid V by one carbon atom to give the nitrile VI
(J. Lip. Res. 29, 1387, 1988). The protective groups are split off by hydrolysis under mild conditions, and 12 - 21~~~~-8 compounds of the type VII are obtained. These nitriles VII can then be reacted directly witH trialkyl-tin azides in a suitable solvent, such as, for example, toluene, at elevated temperatures to give the tetrazole derivatives X. However, it may be advantageous to protect the free OH
groups, for example with THP or MOM protective groups to give the compounds VIII, before the tetrazole formations.
The reaction to give the protected tetrazole derivative IX is carried out under the conditions described above.
Splitting off the protective groups again leads to compounds of the type X.
Equation 2 C~ N
i OHCO H .. OHCO
H
XI R ~ H, OCHO ~ XII R ~ H, OCHO
C!N CAN
HO H w RO ..
H
XIII R - H, OH XIV R ~ H, OTHP, OIAOM
R' - THP, MOM

Equation 2 (continued) 1 N-N,~ 1 iN NON
N
-NH
HO N .- RO
XVI R - H, OH XV R - H, OTHP
To prepare C-23-tetrazolylcholanic acids XVI, for example, iodine compounds of the type XI are converted into nitriles XII by nucleophilic substitution with alkali metal cyanides. After the protective groups have been split off, the nitrites XIII can either be reacted directly to give the tetrazole compounds XVI, or the possible route via protected derivatives XIV and XV can also be utilized.
Equation 3 R R
~2 ~ 1 O~OTHP
0 DH ~ H
H
I ~ N H
NO CN CH 0 TNPO-(CN1CH=0)"
-( z : ).
H R R' %VII %VIII
R' R' Z' 'OH
N ~ OR"
H
1 1"
N N
THPO-(CH=CH=0)" R, THPO-(CH=CN=0)~ N N
H H R' XIX XX

- 14 - ~123Q4~
Equation 3 (continued) R. R. _ N
j T ~N
I1~C~N ~ H'NH
N N
1 1 "' l ! "' TNPO-(CH=CH=0)" H H THPO-(CH1CN=0)~ H H
H R. H R.
xxl xxll R I NvN R N, N 'N H N 'N H
H ~ H
1 l "' H H N N
Hp-(CHlCH~D)" R R"0-(CH=CN=0).
H H R
xxill xxlv H
1 I NON ( I ~ H
N'NH ~ N'NH
H H
1 1-"
H H N N
Ny-(CH=CH=0)~ H=N-(CH=CH=0), R N R
H
xxv xxvl R = H, OH; R' - H, OTHP; R" - mesyl, tosyl; n = 0, 1, 2 C-24-tetrazole derivatives are obtained by providing compounds of the type XVII with protective groups on the hydroxyl groups and on the acid function. The resulting ester functions of compounds of the type XVIII are reduced to primary hydroxyl groups (XIX) . The hydroxyl group is activated by a methane- or toluenesulfonyl radical (XX), and subjected to substitution with an alkali metal cyanides this gives compounds of the type XXI. The reaction of nitrile to give tetrazolyl compounds (XXII) and the subsequent splitting-off of the protective groups to give XXIII are carried out by the processes described above. From the free hydroxyl function in position 3 or in the bonding member X introduced, an amino function can be prepared via compounds of the 212~~ø~

formulae XXIV and XXV analogously to processes already described (EP-A-O 489 423), and compounds of the type XXVI are obtained.
Equation 4 H OH
R'..
H
XXVII XXVIi~
N ~N~ ,N
0 N~N~N H ~ N ~N H
H
--.
R." R' n XXIX XXX
R - H, OH 0 R ' - 0 H H~O
n HO H
n ~ 0, t , 2 - 16 - 212~~48 Equation 4 (continued) R" - H, OCHO, OCOCH3 0 R' ' ' ~ OCHO, OCOCH3 H~O~
H n n ~ 0 , 1 , 2 H3CC00, OHC
H
Tetrazole derivatives can be prepared from natural bile acids or also from modified bile acids, for example from dimeric derivatives, by reacting the free carboxyl group with 5-aminotetrazole, after activation. If the usual peptide coupling reagents are used, the yields of this reaction are low. It is therefore expedient to protect the free hydroxyl functions of the bile acid derivative XXVII, for example with formyl or acetyl protective groups. The acid chloride is produced from the protected compounds XXVIII with phosphorus pentachloride or thionyl chloride, for example in THF. Reaction of the acid chloride with dry 5-aminotetrazole gives compounds of the type XXVIX, from which the end products XXX can be ob-tained by simply splitting off the protective groups.
The compounds have useful pharmacological properties and are therefore particularly suitable as hypolipidemic agents.
The invention also relates to medicaments based on the compounds of the formula (I) and to the use of the compounds as medicaments, in particular for lowering the cholesterol level.
The compounds according to the invention were tested biologically by deteranination of the inhibition of [3H]-taurocholate uptake in brush border membrane vesi-cles of the ileum of rabbits. The inhibition test was carried out as follows:

i, i 1. Preparation of brush border membrane vesicles from the ileum of rabbits.
Brush border membrane vesicles were prepared from the intestinal cells of the small intestine by the so-called Mg'* precipitation method. Male New Zealand rabbits (2 to 2.5 kg bodyweight) were sacrificed by intravenous injection of 0.5 ml of an aqueous solution of 2.5 mg Tetracain HC1, 100 T61R and 25 mg of mebezoaiuat iodide.
The small intestine was rinsed with ice-cold physiologi-cal saline solution. The terminal 7/10 of the small intestine (measured in the oral-rectal direction, i.e.
the terminal ileum, which contains the active Na*-depan-dent bile acid transportation system) was used for preparation of the brush border membrane vesicles. The intestines were frozen in plastic bags under nitrogen at -80°C. For preparation of the membrane vesicles, the frozen intestines were cooled at 30°C in a water-bath.
The mucosa was scraped off and suspended in 60 ml of ice-cold 12 mM Tris/HCl buffer (pH 7.1)/300 mM mannitol, 5 mM
EGTA/10 mg/1 of phenylmethyleulfonylfluoride/lmg/1 of trypsin inhibitor for soya beans (32 U/mg)/0.5 mg/l of trypsin inhibitor from bovine lung (193 U/mg)/5 mg/1 of bacitracin. After dilution to 300 ml with ice-cold distilled water, the mixture was homogenized with an UltraturraxT"" (18-rod, IKA Werk Staufen, FRG) for 3 minutes at 75 % of the maximum powsr, while cooling with ice.
After addition of 3 ml of 1 M MgClz solution (final concentration 10 mM) , the mixture was left to stand at 0°C for exactly 1 minute. Hy addition of Mg'*, the cell membranes aggregate and precipitate, with the exception of the brush border membranes. After centrifugation at 3000 x g (5000 revolutions per minute, SS-34 rotor) for 15 minutes, the precipitate is discarded and the super-natant, which contains the brush border membranes, was centrifuged at 267000 x g (15000 revolutions par minute, SS-34 rotor) for 30 minutes. The superaatant was discarded and the precipitate was rehomogeaized in 60 ml of 12 mM tris/HCl buffer (pH 7.1)/60 mM mannitol, 5 mM

EGTA using a Potter Elvejhem homogenizer (Braun, Melsunger~, 900 revolutions per minute, 10 strokes) . After addition of 0.1 ml of 1 M MgCl, solution and an incubation time of 15 minutes at 0°C, the mixture was centrifuged again at 3000 x g for 15 minutes. The super-natatant was then centrifuged again at 46000 x g (15000 revolutions per minute, SS-34 rotor) for 30 minutes. The precipitate was taken up in 30 ml of 10 mM Tris/Hepes buffer (pH 7.4)/300 mM mannitol and resuspended homo-geneously by 20 strokes in a Potter Elvejhem homogenizer at 1000 revolutions per minute. After centrifugation at 48000 x g (20000 revolutions per minute, SS-34 rotor) for 30 minutes, the precipitate was taken up in 0.5 to 2 ml of Tris/Hepes buffer (pH 7.4)/280 mM mannitol (final concentration 20 mg/ml) and resuspended with the aid of a tuberculin syringe with a 27 gauge needle. The vesicles were either used for transportation studies immediately after preparation or stored at -196°C in 4 mg portions in liquid nitrogen.
2. Inhibition of the Na'-dependent [3H]-taurocholate uptake in the brush border membrane vesicles of the ileum.
The uptake of substrates into the brush border membrane vesicles described above was determined by means of the so-called membrane filtration technique. 10 ~.l of the vesicle suspension (100 ~.g of protein) were pipetted as drops onto the wall of a polystyrene incubation tube (11 x 70 mm) which contained the incubation medium with the corresponding ligands (90 ~1). The incubation medium contained 0 . 75 ~.l = 0 .75 ~.Ci of ['H (G) ] -taurocholate (specific activity: 2.1 Ci/mMol)/0.5 ~l of 10 mM tauro-cholate/8.75 ~1 of sodium transportation buffer (10 mM
Tris/Hepes (pH 7.4)/100 mM mannitol/100 mM NaCl) (Na-T-B) or 8.75 ~.1 of potassium transportation buffer (10 ml Tris/Hepes (pH 7.4)/100 mM mannitol/100 mM RCl) (R-T-B) and 80 ~.1 of the inhibitor solution in question, dis-solved in Na-T buffer or R-T buffer, depending on the i ~ j ~.

experiment. The incubation medium was filtered through a polyvinylidene fluoride membrane filter (SYHV LO 4NS, o .45 Vim, 4 mm m, MilliporeT"", Eschborn, FRG). The transpor-tation measurement was started by mixing the vesicles with the incubation medium. The concentration of tauro-cholate in the incubation batch was 50 ftM. Af ter the desired incubation time (usually 1 minute). the trans-portation was stopped by addition of 1 ml of ice-cold stopping solution (10 mM Tris/Hepea (pH 7.4) /150 mM RCl) .
The resulting mixture was filtered With suction over a membrane filter of cellulose nitrate (ME 25, 0.45 fan, 25 mm diameter, Schleicher ~ Schuell. Dassell, FRG) under a vacuum of 25 to 35 mbar. The filter was rinsed with 5 ml of ice-cold stopping solution.
To measure the uptake of the radioactively labeled taurocholate, the membrane filter was dissolved with 4 ml of the scintillator Quickscint 361 (Zinsser Analytic GmbH, Frankfurt, FRO) and the radioactivity. was measured by liquid scintillation measurement in a Tri-CarbT"" 2500 measuring instrument (Canberra Packard GmbH, Frankfurt, FRG). After calibration of the apparatus with the aid of standard samples and after correction for any chemilumi-nascence present, the values measured were obtained as dpm (decompositions per minute).
The control values were determined in each case in Na-T-H
and K-T-H. The difference between the uptake in Na-T-B
and K-T-B gave the Na'-dependent transportation content.
That concentration of inhibitor at which the Na'-depen-dent transportation content was inhibited by 50 % - based on the control - was designated the ICSONa'.
The pharmacological data compris~ a test series in which the interaction of the compounds according to the inven-tion with the intestinal bile acid transportation system in the terminal small intestine was investigated. The results are summarized in Table 1.

212304 8 _ 2a -The invention furthermore relates to the use of the compounds according to the invention for the preparation of a medicine.
For this, the compounds of the formula I are dissolved or suspended in pharmacologically acceptable organic sol vents, such as mono- or polyhydrate alcohols, such as, for example ethanol or glycerol, or in triacetin, oils, for example sunflower oil or cod-liver oil, ethers, such as, for example, diethylene glycol dimethylether, or else polyethers, for example polyethylene glycol, or also in the presence of other pharmacologically acceptable polymeric carriers, such as, for example, polyvinylpyr-rolidone, or other pharmaceutically acceptable additives, such as starch, cyclodextrin or polysaccharides. The compounds according to the invention furthermore can be administered in combination with the other medicaments.
The compounds of the formula I are administered in various dosage forms, preferably orally in the form of tablets, capsules or liquids. The daily dose varies in the range from 3 mg to 5000 mg, but preferably in the dose range from 10 to 1000 mg, depending on the body weight and constitution of the patient.
Example 1 OHCO ~CN CN
s H 1 ~ --H H
OHCO~"" H .~0CH0 HO
H
The protective groups were removed from 5.0 g (10.9 mmol) of the triformyl compound (J. Lip. Res. 29, 1387, 1988) in 100 ml of 1M NaOMe/MeOH solution at room temperature for 2 hours. For working up, water is added and the methanol is stripped off. The mixture is extracted three times With CH,Ch, dried over MgSO, and concentrated.

212~~4~~

3.9 g (96 %) of the unprotected nitrile are obtained.
MS (FAB, 3-NBA/LiCl) C33H3~NO3 (375) 383 (M+Li') Example 2 CN THPO ~CN
H H
HO H -.- THPO~~. H ~OTHP
3.8 g (10.1 mmol) of trihydroxy compound (Example 1) are dissolved in 40 ml of CHsClz, 20 ml of dihydropyran and 300 mg of pyridinium 4-toluenesulfonate are added at 0°C
and the reaction mixture is then stirred at room tempe-rature for 2 days. It is concentrated and the residue is chromatographed over silica gel (cyclohexane/ethyl acetate 2:1). 5.7 g (90 %) of product are obtained.
MS (FAB, 3-NBA/LiCl) C33HsaNOs 8543) 550 (M + Li') Example 3 THPO THPO ~~N
CN j 1 i ! ~ ~ I \~-H H
H I ~ _ I
H ..~~ H
THPOx ..~~~OTHP THPO~ ~OTHP
H H
2.0 g (3.19 mmol) of nitrile (Example 2) and 2.1 g (6.34 mmol) of tributyl-tin azide are heated under reflux in toluene for 6 days. After 3 days, a further 2.1 g of tributyl-tin azide are added. The reaction mixture is concentrated. After chromatography of the residue (silica gel, ethyl acetate/MeOH 95:5), 1.7 g (79 %) of tetrazole derivative are obtained.
MS (FAB, 3-NBA/LiCl) C38H6sN4O6 (670) 677 (M+Li') Example 4 NON _ NON
-NH -NH
--.
THPO H HO
1.6 g (2.39 amnol) of THP-protected compound (Example 3), 200 mg of pyridinium p-toluenesulfonate and 1.6 ml of acetic acid are heated under reflux in 30 ml of methanol for 12 hours. After cooling, the mixture is concentrated and the residue is chromatographed over silica gel (CHzClZ/MeOH 9:1) . Yield: 720 mg (87 %) MS (FAB, 3-NHA/LiCl) Cs3H38N~O3 (418) 425 (M + Li') Example 5 OHCO OHCO CAN
I
H I ~ _ H I J
E ~\\~ H
H H
OHCO~"' ~'I4~OCH0 OHCO~ H ,~OCHO
H
21 g (36.6 mmol) of iodine compound (Tetrahedron, 45 (17), 5423, 1989) and 3.6 g of sodium cyanide are stirred in 300 ml of DMSO at 50°C for 1.5 hours. The reaction mixture is poured onto ice-water and extracted with ethyl acetate. The organic phase is dried (MgSO,) and concen-trated. Chromatography (cyclohexane/ethyl acetate) gives 15 g (87 %) of nitrite.
MS (FAB, 3-NBA/LiCl) Cz.,H39NO6 (473) 480 (M + Li+) Example 6 CAN ~N
OHCO
g H
t --.
H H
OH CON". H ..~~~~OCHO HO H .."
The protective groups are removed from the compound protected by formyl (Example 5) by the process described for Example 1.
MS (FAB, 3-NHA/LiCl) Cz,,H39NO3 (389) 396 (M + Li') Examples 7, 8 and 9 were prepared by the process des-cribed for Examples 2, 3 and 4.
Example 7 CAN
THPO H _ ....
MS (FAB, 3-NBA/LiCl) C39H63NO6 (641) 648 (M + Li') Example 8 N . N~
NH
N
,,....
THPO H _ ....

22304 s MS (FAB, 3-NBA/LiCl) C39HeeN,,O6 (684.5) 691.6 (M + Li') Example 9 N ~ N~
NH
N
HO H ....
MS (FAB, 3-NHA/LiCl) Cz4H40N~Oj (432) 439 (M + Li'") The compound of Example 9 can also be obtained directly from Example 6 and tributyl-tin azide (see Example 3).
The crude product is chromatographed twice for purifica-tion (1. ethyl acetate/MeOH 9:1; 2.CHzClz/MeOH/acetic acid 9:1:0.1). The yield is 83 %.
Example 10 OAIe OH
HO THPO
H _.. H _ ....
100 g (215 mmol) of trihydroxy compound (EP-A-0 489 423) are protected with tetrahydropyranyl groups according to Example 2. The resulting crude product is reacted without further purification. As a solution in 250 ml of ether, it is slowly added dropwise to a suspension of 15 g of LiAlH,, in 500 ml of ether at 0°C. After 2 hours at 0 to 5°C, water is carefully added and the mixture is then extracted several times with ether. The organic phase is dried (MgSO,,) and concentrated. 100 g (67 %) of product are obtained.

- 25 _ Example 11 OH CAN
THPO THP( H _ . . H
20 g (29.0 a~ol) of the alcohol (Example 10) are dis-solved in 150 ml of pyridine. 2.45 ml (31.0 umnol) of methanesulfonyl chloride are slowly added dropwise at 0°C
and the mixture is then stirred at room temperature for 2 hours. For working up, the mixture is poured onto ice-water and extracted with ethyl acetate. The organic phase is dried (MgSO,,) and concentrated. The crude product is dissolved in 200 ml of DMSO. 3.0 g of NaCN are added and the mixture is stirred at 50°C for 1 hour. It is poured onto ice-water and extracted with ethyl acetate. After the organic phase has been dried, the residue is concen-trated and chromatographed (cyclohexane/ethyl acetate 7:3). 15 g (77 %) of nitrile are obtained.
MS (FAB, 3-NBA/LiCl) C4sH69N0~ (699) 706 (M + Li+) Example 12 THPO ,~
_ \~C~N
H
H I H
THPO~~', H ~4~OTHP
Example 12 was obtained by the process described for Example 11.
Examples 13 to 16 are prepared by the processes described for Examples 3 and 4.

w N
~N
NH
R0~
n n Example R n MS (FA8, 3-NBA/LiCl) 13 THP 0 C,~oHssNsOs (698.5) 705.7 (M +
Li') 14 H 0 C,SH,,sN403 (446) 453 (M + Li') 15 THP 1 C~sH~oN,O~ (742.5) 749.4 (M + Li') 16 H 1 Cz~H,,sNsOs (490) 497 (M + Li') Example 17 HO N
I _ T ,N N
N..~N H
H

I I
HO H H HsC-S-0 0 ~"0 H p _ . .. .
H H
g (30.6 mmol) of the alcohol from Example 16 are dissolved in 200 ml of CH,Clz, 100 ml of pyridine are added and the mixture is reacted with 5 ml of methanesul-10 fonyl chloride at -20 to -10°C for 2.5 hours. The still cold reaction solution is poured onto ice-water and extracted with ethyl acetate, and the organic phase is dried (MgSO,) and concentrated. The crude product is purified over a short silica gel column 15 (CHC13/MeOH 93:7). 16 g (92 %) of product are obtained.
MS (FAB, 3-NBA/LiCl) CzeH48NsO6S (568) 575 (M + Li') Example 18 N
N ~N
NH H
0 -.
I I
H3C-S-0 Ns i 0 H _.. H _..
2.3 g (84.05 a~ol) of mesyl compound (Example 17) and 290 mg of sodium azide are stirred in 30 ml of DMF at 100°C for 2 hours. After cooling, the reaction mixture is concentrated and the residue is chromatographed over silica gel (CHzClz/MeOH 85/15) . The yield is 1.0 g (49 %) .
MS (FAB, 3-NBA/LiCl) C~~H~SN~03 (515) 522 (M + Li') Example 19 N
~N
'N H
N3 H:N ~
_.. H
1.0 g (1.94 mmol) of azido compound are dissolved in 30 ml of methanol/1.5 ml of water and hydrogenated with Hz in the presence of 50 mg of Pd black. The catalyst is filtered off and the filtrate is concentrated. The residue is chromatographed over silica gel (CHzClz/MeOH/NEt, 8:2 :1) , and 550 mg (58 %) of amine are obtained.
MS (FAB, 3-NHA/LiCl) Cz~H,,~Ns03 (489) 496 (M + Li') 212304 ~ - 28 -Example 20 OH
N
H H __ HO ".. 0HC0 0~ 0 H
H
OHCO _'_ ~ ~'' H H
0 N .~'0CH0 H H H
H H
OHCO~~~ H ~~~OCHO
3.0 g (3.76 mmol) of the unprotected compound (EP-A-0 489 423) are dissolved in 30 ml of formic acid, 0.2 ml of perchloric acid is added and the mixture is stirred at 50°C for 2 hours. 30 ml of acetic anhydride are then added dropwise at room temperature, and the mixture is stirred for a further 30 minutes. It is poured onto ice-water and extracted with ethyl acetate. The organic phase is dried and concentrated and the residue is chromatographed. 1.93 g (55 %) of compound protected with formyl are obtained.
MS (FAH, 3-NBA) C53H~9N013 (938) 939 (M + H'") Example 21 OHCO N~N~NH
O~N ~N~
H H
OHCO
H H
O~N ,°OCHO
H H H
H H
OHCON~, H '~''OCHO
0.5 g (0.53 amnol) of Example 20 is dissolved in 30 ml of dry THF, 130 mg (0.62 mmol) of PC15 are added and the mixture is stirred at room temperature for 30 minutes. A
solution of 200 mg (2.35 mmol) of anhydrous 5-aminotetra zole in 10 ml of DMF is added to the reaction solution.
After a further 3 hours at room temperature, the mixture is concentrated and the residue is chromatographed (CHC13/MeOH 9:1). 400 mg (75 ~) of tetrazole compound are obtained.
MS (FAB, 3-NBA/LiCl) Cs4HeoNsOiz (1005) 1012 (M + Li') Example 22 .._ N~Nv ~.~ NH
~N~
N
H
N
H H
HO
H
To split off the formyl groups, 370 mg (0.37 amaol) of Example 21 are dissolved in 20 ml of EtOH, 2 ml of 1N NaOH solution are added and the mixture is stirred at room temperature for 6 hours. It is then concentrated, water is added and 1N HC1 is added to -- pH 2. The ~~23048..

precipitate is filtered off with suction and dissolved in methanol, the solution is filtered and the filtrate is concentrated again. 170 mg (53 %) of product are obtained.
MS (FAH, 3-NBA/LiCl) C,,9H~oN60~ (865) 872 (M + Li+) Examples 23 and 24 are prepared from triformylcholic acid by the processes described for Examples 21 and 22.
_ _ N,~-N H
~~ N
N N
H
RO H _..
Example R MS (FAB, 3-NBA/LiCl) 23 -CHO Cs8H~1Ns0~ (559) 566 (M + Li') 24 H CzsHmNsO,, (475) 482 (M + Li') Example 25 Chc N
N
'N H
HO H _..
150 mg (0.31 mmol) of amino compound (Example 19), 130 mg (0.32 mmol) of cholic acid, 80 mg (0.64 mmol) of hydroxy-benzotriazole and 65 mg (0.32 a~ol) of dicyclohexylcarbo-diimide are stirred in 20 ml of THF at room temperature for 24 hours. The reaction mixture is concentrated and the residue is chromatographed over silica gel (CHzCla/MeOH/NEt3 8:2:1) . 250 mg (88 %) of product are obtained.
MS (FAB, 3-NBA/LiCl) CslH8sNs0~ (880) 887 (M + Li') Examples 26 and 27 are obtained by the process described for Example 25.

212344 ~

N
N
NH
HO
Example R MS (FAB, 3-NBA/LiCl) 26 a-OH CslHBSNsOs (864) 865 (M + H*) 27 ~B-OH CslHBSNsOs (864) 865 (M + H*) Example 28 OH CN
HO H _.. HO H _ .
2.0 g (4.9 mmol) of cholic acid and 5 ml (36 mmol) of triethylamine are dissolved in 150 ml of THF, and 1.5 ml (16 mmol) of ethyl chloroformate are added at 0°C. After minutes, 1.5 g (16 mmol) of aminoacetonitrile hydro-chloride are added. The mixture is stirred at room 10 temperature for 5 hours. The precipitate formed is filtered off and the solution is concentrated. After chromatography of the crude product (chloroform/methanol 17:3), 1.8 g of product (85 %) are obtained.
MS (FAB, 3-NHA/LiCl) Cz6H,~sN,O, (446) 453 (M + Li*) Example 29 N
~~ N ~ ~~ N
N~N~
H
HO H _.. N N
1.5 g (3.36 mmol) of the nitrile and 3.5 g (10.5 mmol) of tributyltin azide are heated under reflex in 100 ml of toluene for 24 hours. When the reaction has ended, the mixture is concentrated in vacuo and the residue is chromatographed over silica gel (CHC13/MeOH 7:3). 830 mg (56 %) of product are obtained.
MS (FAB, 3-NBA) CZ6H43N5~4 (489) 490 (M + H'') Examples 30 and 31 were prepared by the processes des-cribed for Examples 28 and 29.
R
HO H
N
H
H

Example R MS (FLAB, 3-NHA/LiCl) 30 Ni-~.C N CsoHaiNsO~ (835. 6) 842 . 6 (M
+ Li') H

31 N CsoHasNsO~ (878. 6) 891.7 (M +
2Li-N
ON

H+) N-N H

Table 1 shows measurement values for the inhibition of the ['H]-taurocholate uptake in brush border membrane vesicles of the ileum from rabbits. The quotients of the ICso and ICso~, values of the reference substance tauro-chenodeoxycholate (TCDC) and the particular test sub-stance are stated.
Table 1 Compounds ICso-TCDC (~mol] ICsox. TCDC [~mol]
from Example ICso-substanc~ [~Cmol]=C oxa-substance I~mol]

4 0.28 0.38 14 1.20 1.35 16 0.26 0.25 22 1.66 1.35 24 0.97 1.27 27 0.26 0.25 29 0.46 0.48 2 31 1.39 1.24

Claims (6)

1. A tetrazole-bile acid derivative of the formula I

in which G1 is H or a radical of the formula II

in which R(1) is H, formyl, acetyl, benzoyl, methoxymethyl or tetrahydropyranyl, R(2) to R(5), where R(2) and R(3) or R(4) and R(5) in each case together are the oxygen of a carbonyl group, or individually and independently of one another are H, OH, O-formyl, O-acetyl, O-benzoyl, methoxymethoxy or tetrahydropyranyloxy, G2 is a radical of the formula IV

in which where m is 1 to 3, n is zero or 1 and o is zero, 1 or 2, and R(6) to R(9) have the meaning given above under R(2) to R(5), and X is a bridge group or a covalent bond.

2. A compound of the formula I as claimed in claim 1, in which X is a covalent bond or one of the following bridge groups

3. A process for the preparation of a compound of the formula I as claimed is claim 1, which comprises a) in the case where X = a single bond, reacting suitable reactive forms of G1 and G2 with one another by processes which are known in princi-ple, or b) in the case where X = a bridge group, reacting a) reactive forms of G1-X with G2 or b) reactive forms of G2-X with G1 with one another by processes which are known in principle.

4. A medicament comprising a tetrazole-bile acid derivative as claimed in claim 1 and a pharmaceutically acceptable carrier.

5. A hypolipidemic agent comprising a tetrazole-bile acid derivative as claimed in claim 1 and a pharmaceutically acceptable carrier.

6. The use of a tetrazole-bile acid derivative as claimed in claim 1 for lowering lipid levels.