CA2125410A1 - Maturation, desiccation and encapsulation of gymnosperm somatic embryos - Google Patents

Maturation, desiccation and encapsulation of gymnosperm somatic embryos

Info

Publication number
CA2125410A1
CA2125410A1 CA 2125410 CA2125410A CA2125410A1 CA 2125410 A1 CA2125410 A1 CA 2125410A1 CA 2125410 CA2125410 CA 2125410 CA 2125410 A CA2125410 A CA 2125410A CA 2125410 A1 CA2125410 A1 CA 2125410A1
Authority
CA
Canada
Prior art keywords
somatic embryos
osmotic
water stress
water
moisture content
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2125410
Other languages
French (fr)
Other versions
CA2125410C (en
Inventor
Stephen M. Attree
Lawrence C. Fowke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Saskatchewan
Original Assignee
Stephen M. Attree
Lawrence C. Fowke
University Of Saskatchewan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stephen M. Attree, Lawrence C. Fowke, University Of Saskatchewan filed Critical Stephen M. Attree
Priority to CA002238373A priority Critical patent/CA2238373A1/en
Publication of CA2125410A1 publication Critical patent/CA2125410A1/en
Application granted granted Critical
Publication of CA2125410C publication Critical patent/CA2125410C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H7/00—Gymnosperms, e.g. conifers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A01H4/006—Encapsulated embryos for plant reproduction, e.g. artificial seeds

Abstract

Desiccated mature gymnosperm somatic embryos, preferably coniferous somatic embryos having a moisture content of less than about 55%, are prepared by water stressing immature embryos in a medium comprising a metabolizable carbon source and a suitable growth regulator influencing embryo development, for a period of time and under conditions sufficient to yield mature viable somatic embryos having a moisture content in the specified range.
The water stressing may be environmental, as by controlled declining relative humidity. Or a suitable osmotic or non-osmotic water stress agent can be used. The use of a non-permeating osmotic water stress agent such as polyethylene glycol of molecular weight at least about 1,000 is preferred, in which case the water stress should simulate a non-osmotic water stress. An example of a suitable preferred growth regulator is abscisic acid.
CA002125410A 1991-12-19 1992-12-18 Maturation, desiccation and encapsulation of gymnosperm somatic embryos Expired - Lifetime CA2125410C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002238373A CA2238373A1 (en) 1991-12-19 1992-12-18 Desiccation-tolerant gymnosperm embryos

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US81017191A 1991-12-19 1991-12-19
US810,171 1991-12-19
PCT/CA1992/000549 WO1993011660A2 (en) 1991-12-19 1992-12-18 Maturation, desiccation and encapsulation of gymnosperm somatic embryos

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002238373A Division CA2238373A1 (en) 1991-12-19 1992-12-18 Desiccation-tolerant gymnosperm embryos

Publications (2)

Publication Number Publication Date
CA2125410A1 true CA2125410A1 (en) 1993-06-24
CA2125410C CA2125410C (en) 2000-03-28

Family

ID=25203192

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002125410A Expired - Lifetime CA2125410C (en) 1991-12-19 1992-12-18 Maturation, desiccation and encapsulation of gymnosperm somatic embryos

Country Status (10)

Country Link
US (3) US5985667A (en)
EP (1) EP0618766B1 (en)
AT (1) ATE202261T1 (en)
AU (1) AU679435B2 (en)
CA (1) CA2125410C (en)
DE (1) DE69231890T2 (en)
FI (1) FI942909A (en)
NO (1) NO942324D0 (en)
NZ (1) NZ246137A (en)
WO (1) WO1993011660A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999063805A2 (en) 1998-06-05 1999-12-16 University Of Saskatchewan Technologies Inc. Increasing levels of growth regulator and/or water stress during embryo development

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WO1999063805A2 (en) 1998-06-05 1999-12-16 University Of Saskatchewan Technologies Inc. Increasing levels of growth regulator and/or water stress during embryo development

Also Published As

Publication number Publication date
AU679435B2 (en) 1997-07-03
US5985667A (en) 1999-11-16
FI942909A (en) 1994-08-17
US5464769A (en) 1995-11-07
EP0618766B1 (en) 2001-06-20
CA2125410C (en) 2000-03-28
AU3154193A (en) 1993-07-19
ATE202261T1 (en) 2001-07-15
DE69231890D1 (en) 2001-07-26
NO942324L (en) 1994-08-17
FI942909A0 (en) 1994-06-17
NZ246137A (en) 1996-04-26
WO1993011660A3 (en) 1993-09-16
DE69231890T2 (en) 2002-03-28
EP0618766A1 (en) 1994-10-12
NO942324D0 (en) 1994-06-17
WO1993011660A2 (en) 1993-06-24
US6372496B1 (en) 2002-04-16

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