CA2146235A1 - Process for growing thraustochytriales - Google Patents

Process for growing thraustochytriales

Info

Publication number
CA2146235A1
CA2146235A1 CA002146235A CA2146235A CA2146235A1 CA 2146235 A1 CA2146235 A1 CA 2146235A1 CA 002146235 A CA002146235 A CA 002146235A CA 2146235 A CA2146235 A CA 2146235A CA 2146235 A1 CA2146235 A1 CA 2146235A1
Authority
CA
Canada
Prior art keywords
schizochytrium
thraustochytrium
mixtures
microns
culture medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002146235A
Other languages
French (fr)
Other versions
CA2146235C (en
Inventor
William R. Barclay
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
William R. Barclay
Phycotech, Inc.
Omegatech, Inc.
Martek Biosciences Boulder Corporation
Martek Biosciences Corporation
Dsm Ip Assets B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25506004&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2146235(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by William R. Barclay, Phycotech, Inc., Omegatech, Inc., Martek Biosciences Boulder Corporation, Martek Biosciences Corporation, Dsm Ip Assets B.V. filed Critical William R. Barclay
Publication of CA2146235A1 publication Critical patent/CA2146235A1/en
Application granted granted Critical
Publication of CA2146235C publication Critical patent/CA2146235C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/20—Shaping or working-up of animal feeding-stuffs by moulding, e.g. making cakes or briquettes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/25—Shaping or working-up of animal feeding-stuffs by extrusion
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/05—Mashed or comminuted pulses or legumes; Products made therefrom
    • A23L11/07—Soya beans, e.g. oil-extracted soya bean flakes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/40—Meat products; Meat meal; Preparation or treatment thereof containing additives
    • A23L13/42—Additives other than enzymes or microorganisms in meat products or meat meals
    • A23L13/43—Addition of vegetable fats or oils; Addition of non-meat animal fats or oils; Addition of fatty acids
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/50—Poultry products, e.g. poultry sausages
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L15/00—Egg products; Preparation or treatment thereof
    • A23L15/20—Addition of proteins, e.g. hydrolysates, fats, carbohydrates, natural plant hydrocolloids; Addition of animal or vegetable substances containing proteins, fats, or carbohydrates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • A23L17/40—Shell-fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • A23L17/65—Addition of, or treatment with, microorganisms or enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/09—Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L25/00—Food consisting mainly of nutmeat or seeds; Preparation or treatment thereof
    • A23L25/30—Mashed or comminuted products, e.g. pulp, pastes, meal, powders; Products made therefrom, e.g. blocks, flakes, snacks; Liquid or semi-liquid products
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/065—Microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
    • A23L33/12—Fatty acids or derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/20—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/20—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
    • A61K31/202—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids having three or more double bonds, e.g. linolenic
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/10—Protozoa; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S426/00—Food or edible material: processes, compositions, and products
    • Y10S426/805—Pet food for dog, cat, bird, or fish
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S426/00—Food or edible material: processes, compositions, and products
    • Y10S426/807—Poultry or ruminant feed
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/946—Microorganisms using algae

Abstract

Disclosed is a process for growing the microflora Thraustochytrium, Schizochytrium and mixtures thereof, which includes the growing of the microflora in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate.
In a preferred embodiment of the present invention, the process produces microflora having a cell aggregate size useful for the production of food products for use in aquaculture. Further disclosed is a food product which includes Thraustochytrium, Schizochytrium, and mixtures thereof, and a component selected from flaxseed, rapeseed, soybean and avocado meal. Such a food product includes a balance of long chain and short chain omega-3 highly unsaturated fatty acids.

Claims (37)

What is claimed is:
1. A process for growing Thraustochytrium, Schizochytrium, and mixtures thereof, comprising growing said Thraustochytrium, Schizochytrium, and mixtures thereof, in a culture medium containing less than about 3 grams of chloride per liter of said culture medium, sources of carbon, nitrogen, micronutrients, and a non-chloride sodium salt at a temperature from about 5°C to about 48°C and at a pH from about pH 5.0 to about pH 11Ø
2. The process of claim 1, wherein said sodium salt is selected from the group consisting of sodium sulfate, soda ash, sodium oxide, sodium carbonate, sodium bicarbonate, sodium sulfate and mixtures thereof.
3. The process of claim 2, wherein the concentration of said sodium sulfate, expressed as grams of sodium per liter of said culture medium, is greater than about 1Ø
4. The process of claim 2, wherein the concentration of said sodium sulfate, expressed as grams of sodium per liter of said culture medium, is between about 1.0 and about 50Ø
5. The process of claim 2, wherein the concentration of said sodium sulfate, expressed as grams of sodium per liter of said culture medium, is between about 2.0 and about 25Ø
6. The process of claim 1, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof, have all of the identifying characteristics of a biologically pure microorganism selected from the group consisting of ATCC

Nos. 20888 and 20889, and mutant strains derived therefrom, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof, have an omega-3 highly unsaturated fatty acid content of at least about 0.5% dry weight.
7. The process of claim 1, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof, have a sterol content of at least about 0.1% ash free dry weight.
8. The process of Claim 7, wherein Thraustochytrium, Schizochytrium, and mixtures thereof, have a cholesterol content of at least about 15% of the total sterol content.
9. The process of Claim 1, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof, have a cell aggregate size less than about 150 microns in diameter.
10. The process of Claim 1, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof, have a cell aggregate size less than about 100 microns in diameter.
11. The process of Claim 1, wherein said
Thraustochytrium, Schizochytrium, and mixtures thereof, have a cell aggregate size less than about 50 microns in diameter.
13. The process of Claim 1, wherein said culture medium has a chloride concentration of less than about 250 milligrams of chloride per liter of culture medium.
14. A microfloral biomass comprising Thraustochytrium, Schizochytrium, and mixtures thereof, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof have a cell aggregate size less than about 150 microns.
15. The microfloral biomass of Claim 14, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof have been grown in a culture medium containing sodium sulfate.
16. The microfloral biomass of Claim 14, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof, have all of the identifying characteristics of an organism selected from the group consisting of ATCC Nos. 20888 and 20889, and mutants thereof, wherein said mutants have an omega-3 HUFA content of at least about 0.5% dwt.
17. The microfloral biomass of Claim 14, wherein of said Thraustochytrium, Schizochytrium, and mixtures thereof have a sterol content of at least about 0.1% afdw.
18. The microfloral biomass of Claim 17, wherein Thraustochytrium and Schizochytrium have a cholesterol content of at least about 15% of the total sterol content.
19. The microfloral biomass of Claim 14, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof have a cell aggregate size less than about 100 microns.
20. The microfloral biomass of Claim 14, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof have a cell aggregate size less than about 50 microns.
21. A method to produce shrimp, comprising feeding microflora selected from the group consisting of Thraustochytrium, Schizochytrium, and mixtures thereof to larval shrimp, said microflora having a cell aggregate size less than about 150 microns.
22. The method of Claim 21, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof have been grown in a culture medium containing sodium sulfate.
23. The method of Claim 21, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof, have all of the identifying characteristics of an organism selected from the group consisting of ATCC Nos. 20888 and 20889, and mutants thereof, wherein said mutants have an omega-3 HUFA content of at least about 0.5% dry weight.
24. The method of Claim 21, wherein said Thraustochytrium and Schizochytrium have a sterol content of at least about 0.1% afdw.
25. The method of Claim 24, wherein said Thraustochytrium and Schizochytrium have a cholesterol content of at least about 15% of the total sterol content.
26. The method of Claim 21, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof have a cell aggregate size less than about 100 microns.
27. The method of Claim 21, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof have a cell aggregate size less than about 50 microns.
28. A food product comprising:
a) microflora selected from the group consisting of Thraustochytrium, Schizochytrium, and mixtures thereof; and b) a nutrient selected from the group consisting of flaxseed, rapeseed, soybean, avocado meal, and mixtures thereof.
29. The food product of Claim 28, wherein said composition comprises between about 5% and about 95% by weight of said microflora.
30. The food product of Claim 28, wherein said composition comprises between about 5% and about 95% by weight of flaxseed.
31. The food product of Claim 28, wherein said composition comprises between about 5% and about 95% by weight of rapeseed.
32. The food product of Claim 28, wherein said composition comprises between about 5% and about 95% by weight of soybean.
33. The food product of Claim 28, wherein said composition comprises between about 5% and about 95% by weight of avocado meal.
34. The food product of Claim 28, wherein said food product is an extruded product.
35. A method of aquaculture, comprising feeding microflora selected from the group consisting of Thraustochytrium, Schizochytrium, and mixtures thereof to organisms selected from the group consisting of larval shrimp, brine shrimp, rotifers and mollusks, said microflora having a cell aggregate size less than about 150 microns.
36. The method of Claim 35, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof have been grown in a culture medium containing sodium sulfate.
37. The method of Claim 35, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof, have all of the identifying characteristics of an organism selected from the group consisting of ATCC Nos. 20888 and 20889, and mutants thereof, wherein said mutants have an omega-3 HUFA content of at least about 0.5% dry weight.
CA2146235A 1992-10-16 1993-10-12 Process for growing thraustochytriales Expired - Lifetime CA2146235C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/962,522 1992-10-16
US07/962,522 US5340742A (en) 1988-09-07 1992-10-16 Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
PCT/US1993/009679 WO1994008467A1 (en) 1992-10-16 1993-10-12 Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids

Publications (2)

Publication Number Publication Date
CA2146235A1 true CA2146235A1 (en) 1994-04-28
CA2146235C CA2146235C (en) 2011-12-20

Family

ID=25506004

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2146235A Expired - Lifetime CA2146235C (en) 1992-10-16 1993-10-12 Process for growing thraustochytriales

Country Status (11)

Country Link
US (11) US5340742A (en)
EP (5) EP0669809B2 (en)
JP (6) JP3127161B2 (en)
AT (3) ATE241914T1 (en)
AU (2) AU687016B2 (en)
CA (1) CA2146235C (en)
DE (3) DE69334303D1 (en)
DK (3) DK0669809T3 (en)
ES (3) ES2338348T3 (en)
PT (3) PT1707061E (en)
WO (1) WO1994008467A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9745538B2 (en) 2013-12-20 2017-08-29 MARA Renewables Corporation Methods of recovering oil from microorganisms
US11001782B2 (en) 2013-12-20 2021-05-11 Dsm Nutritional Products Ag Methods of recovering oil from microorganisms

Families Citing this family (218)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060094089A1 (en) * 1988-09-07 2006-05-04 Martek Biosciences Corporation Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
US5340742A (en) * 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US6977167B2 (en) * 1988-09-07 2005-12-20 Martek Biosciences Corporation Mixtures of omega-3 and omega-6 highly unsaturated fatty acids from euryhaline microorganisms
US5985348A (en) * 1995-06-07 1999-11-16 Omegatech, Inc. Milk products having high concentrations of omega-3 highly unsaturated fatty acids
US6451567B1 (en) * 1988-09-07 2002-09-17 Omegatech, Inc. Fermentation process for producing long chain omega-3 fatty acids with euryhaline microorganisms
US7033584B2 (en) * 1988-09-07 2006-04-25 Omegatech, Inc. Feeding Thraustochytriales to poultry for increasing omega-3 highly unsaturated fatty acids in eggs
US6410281B1 (en) 1992-07-10 2002-06-25 Omegatech, Inc. Reducing corrosion in a fermentor by providing sodium with a non-chloride sodium salt
US5583019A (en) * 1995-01-24 1996-12-10 Omegatech Inc. Method for production of arachidonic acid
WO1996033263A1 (en) * 1995-04-17 1996-10-24 JAPAN, represented by DIRECTOR-GENERAL OF AGENCY OF INDUSTRIAL SCIENCE AND TECHNOLOGY Novel microorganisms capable of producing highly unsaturated fatty acids and process for producing highly unsaturated fatty acids by using the microorganisms
US20080175953A1 (en) * 1995-06-07 2008-07-24 Martek Biosciences Corporation Process for the Heterotrophic Production of Microbial Products with High Concentrations of Omega-3 Highly Unsaturated Fatty Acids
DK0894142T4 (en) * 1996-03-28 2014-02-24 Dsm Ip Assets Bv Microbial oil comprising a polyunsaturated fatty acid and process for producing oil from pasteurized and granulated biomass.
CA2201652C (en) * 1996-04-04 2007-09-11 The University Of Saskatchewan Use of purified sdg as an antioxidant
EE04063B1 (en) 1996-07-23 2003-06-16 Nagase Biochemicals, Ltd. Method for the preparation of docosahexaenoic acid and docosapentaenoic acid
US6566583B1 (en) * 1997-06-04 2003-05-20 Daniel Facciotti Schizochytrium PKS genes
JP2001512029A (en) * 1997-08-01 2001-08-21 マーテック・バイオサイエンスィズ・コーポレーション DHA-containing nutritional compositions and methods for their production
EP1021083B1 (en) 1997-08-14 2003-05-07 Martek Biosciences Boulder Corporation A method for increasing incorporation efficiency of omega-3 highly unsaturated fatty acid in poultry meat
US7217856B2 (en) 1999-01-14 2007-05-15 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US20070244192A1 (en) * 1999-01-14 2007-10-18 Martek Biosciences Corporation Plant seed oils containing polyunsaturated fatty acids
US7247461B2 (en) * 1999-01-14 2007-07-24 Martek Biosciences Corporation Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof
US7211418B2 (en) * 1999-01-14 2007-05-01 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US8003772B2 (en) 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
GB9910461D0 (en) * 1999-05-07 1999-07-07 Ewos Ltd Pigment
KR100338193B1 (en) * 1999-12-13 2002-05-24 김광양 New kind of protista schizochytrium sp. Sπ-2(KCTC 0694BP) and a method of cultivation
US6395778B1 (en) 2000-01-11 2002-05-28 Omegatech, Inc. Process for making an enriched mixture of polyunsaturated fatty acid esters
EP1252324B1 (en) 2000-01-19 2010-10-20 Martek Biosciences Corporation Solventless extraction process
EP2341126A3 (en) * 2000-01-28 2011-10-05 Martek Biosciences Corporation Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors
CA2401688A1 (en) * 2000-02-24 2001-08-30 Thomas Veach Ii Long Process for production of carotenoids, xanthophylls and apo-carotenoids utilizing eukaryotic microorganisms
JP3425622B2 (en) * 2000-03-30 2003-07-14 独立行政法人産業技術総合研究所 Cultures containing polyunsaturated fatty acids and methods for producing oils and fats containing polyunsaturated fatty acids using Labyrinthula
US20060025781A1 (en) * 2001-01-17 2006-02-02 Young Wayne P Laparoscopic instruments and methods utilizing suction
TWI377253B (en) 2001-04-16 2012-11-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
US7045683B2 (en) * 2001-05-04 2006-05-16 Abbott Laboratories Δ4-desaturase genes and uses thereof
FR2830416B1 (en) * 2001-10-10 2004-04-16 Valorex Sa FOOD FOR HUMAN NUTRITION
US6851387B2 (en) * 2002-02-15 2005-02-08 Automated Shrimp Holding Corporation Aquaculture method and system for producing aquatic species
US6615767B1 (en) * 2002-02-15 2003-09-09 Automated Shrimp Corporation Aquaculture method and system for producing aquatic species
DE10310021A1 (en) 2002-02-28 2003-10-23 Lukowski Gerold Micro- or nanoparticles of biomass of lipid-containing marine organisms, useful as pharmaceutical or cosmetic active agents or food additives, e.g. for preventing binding of bacteria to skin or tissue
KR100379075B1 (en) * 2002-03-07 2003-04-08 Jinis Biopharmaceuticals Co Method for producing low cholesterol animal food product and food product therefrom
US20040203120A1 (en) * 2002-03-22 2004-10-14 Council Of Scientific And Industrial Research Method for enhancing levels of polyunsaturated fatty acids in thraustochytrid protists
EP2266525B1 (en) * 2002-05-03 2012-07-11 Martek Biosciences Corporation High quality lipids and methods for producing by enzymatic liberation from biomass
US6953574B2 (en) * 2002-06-21 2005-10-11 Technology Commercialization, Inc. Method for producing a fermented hydrolyzed medium containing microorganisms
AU2003227950A1 (en) * 2002-07-27 2004-02-16 Avecia Limited Ink
JP2004083428A (en) * 2002-08-23 2004-03-18 Yoshihara Oil Mill Ltd Food product and medicine with antithrombotic activity and/or antiarteriosclerotic activity
JP4280158B2 (en) 2002-12-27 2009-06-17 富士フイルム株式会社 Microorganisms capable of producing docosahexaenoic acid and use thereof
US20060265766A1 (en) * 2003-03-19 2006-11-23 Advanced Bionutrition Corporation Fish and the production thereof
MXPA05010214A (en) * 2003-03-26 2005-12-14 Martek Biosciences Corp Pufa polyketide synthase systems and uses thereof.
US20050019880A1 (en) * 2003-03-31 2005-01-27 Council Of Scientific And Industrial Research Method of enhancing levels of polyunsaturated fatty acids in thraustochytrid protists
EP1617730A1 (en) * 2003-04-25 2006-01-25 The University of Newcastle Biomass containing animal feed
US6846942B2 (en) * 2003-05-20 2005-01-25 David Rubin Method for preparing pure EPA and pure DHA
AU2003902794A0 (en) * 2003-06-03 2003-06-19 Agresearch Limited Improvements in grass endophytes
US20070015263A1 (en) * 2003-09-01 2007-01-18 Mogens Wumpelmann Method for increasing yield of biomass of and/or components of biomass from marine microorganisms
AU2004280575B8 (en) * 2003-10-02 2010-05-13 Dsm Ip Assets B.V. Production of high levels of DHA in microalgae using modified amounts of chloride and potassium
DE10352838A1 (en) * 2003-11-10 2005-07-07 Nutrinova Nutrition Specialties & Food Ingredients Gmbh A method of cultivating microorganisms of the genus Thraustochytriales using an optimized low salt medium
KR20060130577A (en) * 2003-12-03 2006-12-19 사노피 파스퇴르 리미티드 Cryo-protective agents for microorganisms
US7138391B2 (en) * 2004-08-09 2006-11-21 Silverbrook Research Pty Ltd Hydrophilizable and hydrophilic cyanine dyes
US7114461B2 (en) * 2004-09-28 2006-10-03 Winterlab Limited Method for raising aquatic animals
EP1833313A2 (en) * 2004-10-15 2007-09-19 Corporation Limited Photonz Compositions containing high omega-3 and low saturated fatty acid levels
WO2006047445A2 (en) * 2004-10-22 2006-05-04 Martek Biosciences Corporation Process for preparing materials for extraction
US7588931B2 (en) 2004-11-04 2009-09-15 E. I. Du Pont De Nemours And Company High arachidonic acid producing strains of Yarrowia lipolytica
EP2447356B1 (en) 2005-06-07 2016-04-20 DSM Nutritional Products AG Eukaryotic microorganisms for producing lipids and antioxidants
MX300085B (en) 2005-07-01 2012-06-08 Martek Biosciences Corp Polyunsaturated fatty acid-containing oil product and uses and production thereof.
JP5214464B2 (en) 2005-12-28 2013-06-19 アドバンスド バイオニュートリション コーポレーション Delivery medium for probiotic bacteria, in the form of glass, comprising a dry matrix of polysaccharides, saccharides and polyols and method for producing the same
US8968721B2 (en) 2005-12-28 2015-03-03 Advanced Bionutrition Corporation Delivery vehicle for probiotic bacteria comprising a dry matrix of polysaccharides, saccharides and polyols in a glass form and methods of making same
DE602006015701D1 (en) * 2005-12-29 2010-09-02 Abl Biotechnologies Ltd NEW SCHIZOCHYTRIUM LIMACINUM TRUNK, SUITABLE FOR THE PRODUCTION OF LIPIDES AND EXTRACELLULAR POLYSACCHARIDES, AND METHOD THEREFOR
EP2004835A4 (en) * 2006-03-15 2011-02-23 Martek Biosciences Corp Plant seed oils containing polyunsaturated fatty acids
CA2648266A1 (en) * 2006-04-03 2007-10-18 Advanced Bionutrition Corporation Feed formulations containing docosahexaenoic acid
US20070243307A1 (en) * 2006-04-11 2007-10-18 Martek Biosciences Corporation Food Products Comprising Long Chain Polyunsaturated Fatty Acids and Methods for Preparing the Same
KR100700486B1 (en) * 2006-05-03 2007-03-28 대상 주식회사 Microorganism producing omega-3 unsaturated fatty acid and processes for preparing omega-3 unsaturated fatty acid using the same
GB2437909A (en) * 2006-05-12 2007-11-14 Advanced Bionutrition Inc Animal feed comprising docosahexaenois acid from a microbial source
AU2007260873B2 (en) 2006-06-22 2013-05-02 Dsm Ip Assets B.V. Encapsulated labile compound compositions and methods of making the same
EP2043970B1 (en) * 2006-07-05 2018-04-18 Centre National De La Recherche Scientifique (C.N.R.S.) Iron-copper co-catalyzed process for carbon-carbon or carbon-heteroatom bonding
MX2009001346A (en) * 2006-08-01 2009-04-17 Ocean Nutrition Canada Ltd Oil producing microbes and methods of modification thereof.
WO2008027991A2 (en) 2006-08-29 2008-03-06 Martek Biosciences Corporation USE OF DPA(n-6) OILS IN INFANT FORMULA
KR100860111B1 (en) * 2006-10-02 2008-09-25 인제대학교 산학협력단 Newly isolated Thraustochytrium sp. KJS-1, Bacillus polyfermenticus KJS-2 and Feed additives for cultivated fish including Thraustochytrium sp. KJS-1, Bacillus polyfermenticus KJS-2 and Bacillus licheniformis, Feed containing above feed additives
WO2008049512A1 (en) * 2006-10-27 2008-05-02 Lonza Ag Production of omega-3 fatty acids in microflora of thraustochytriales using modified media
CA2673120C (en) 2006-12-18 2012-08-07 Advanced Bionutrition Corporation A dry food product containing live probiotic
MY154965A (en) * 2007-06-01 2015-08-28 Solazyme Inc Production of oil in microorganisms
US8652814B2 (en) * 2007-06-04 2014-02-18 National University Corporation Hokkaido University Method for production of DHA-containing phospholipid through microbial fermentation
AU2008269989B2 (en) * 2007-06-29 2014-02-27 Dsm Ip Assets B.V. Production and purification of esters of polyunsaturated fatty acids
CN113846129A (en) * 2007-09-12 2021-12-28 帝斯曼知识产权资产管理有限公司 Biological oil and preparation and application thereof
US8343753B2 (en) * 2007-11-01 2013-01-01 Wake Forest University School Of Medicine Compositions, methods, and kits for polyunsaturated fatty acids from microalgae
EP2211881A4 (en) * 2007-11-01 2012-01-04 Wake Forest University School Of Medicine Compositions and methods for prevention and treatment of mammalian diseases
EP2071019A1 (en) * 2007-12-15 2009-06-17 Lonza AG Method for the cultivation of microoranisms of the order thraustochytriales
ES2333571B1 (en) * 2008-03-07 2010-10-21 Universidad De Las Palmas De Gran Canaria PROCEDURE FOR THE PRODUCTION OF BIOMASS RICH IN POLYINSATURATED FATTY ACIDS FROM THE GROWTH OF A THRAUSTOCHYTRIDO.
EP2105506A1 (en) * 2008-03-26 2009-09-30 Lonza Ag Process for preparing oils containing PUFAs employing microorganisms of the order Labyrinthulomycota
NZ588586A (en) 2008-04-09 2012-07-27 Solazyme Inc Direct chemical modification of microbial biomass and microbial oils
US20100297292A1 (en) * 2008-10-14 2010-11-25 Solazyme, Inc. Reduced Pigmentation Microalgae Strains and Products Therefrom
US20100297323A1 (en) * 2008-10-14 2010-11-25 Solazyme, Inc. Gluten-free Foods Containing Microalgae
US20100297296A1 (en) * 2008-10-14 2010-11-25 Solazyme, Inc. Healthier Baked Goods Containing Microalgae
US20100297331A1 (en) * 2008-10-14 2010-11-25 Solazyme, Inc. Reduced Fat Foods Containing High-Lipid Microalgae with Improved Sensory Properties
US20100239712A1 (en) * 2008-10-14 2010-09-23 Solazyme, Inc. Food Compositions of Microalgal Biomass
US20100303989A1 (en) * 2008-10-14 2010-12-02 Solazyme, Inc. Microalgal Flour
US20100303957A1 (en) * 2008-10-14 2010-12-02 Solazyme, Inc. Edible Oil and Processes for Its Production from Microalgae
US20100303961A1 (en) * 2008-10-14 2010-12-02 Solazyme, Inc. Methods of Inducing Satiety
US20100297325A1 (en) * 2008-10-14 2010-11-25 Solazyme, Inc. Egg Products Containing Microalgae
US20100297295A1 (en) * 2008-10-14 2010-11-25 Solazyme, Inc. Microalgae-Based Beverages
US20100303990A1 (en) * 2008-10-14 2010-12-02 Solazyme, Inc. High Protein and High Fiber Algal Food Materials
US8809037B2 (en) 2008-10-24 2014-08-19 Bioprocessh20 Llc Systems, apparatuses and methods for treating wastewater
MX2011005630A (en) 2008-11-28 2011-09-28 Solazyme Inc Manufacturing of tailored oils in recombinant heterotrophic microorganisms.
US20110306102A1 (en) * 2009-02-25 2011-12-15 V. B. Medicare Pvt. Ltd. Improved methods for fermentative production of docosahexaenoic acid
TWI548648B (en) 2009-03-16 2016-09-11 Ｄｓｍ智慧財產有限公司 Protein production in microorganisms of the phylum labyrinthulomycota
TWI600762B (en) * 2009-03-19 2017-10-01 Ｄｓｍ智慧財產有限公司 Polyunsaturated fatty acid synthase nucleic acid molecules and polypeptides, compositions, and methods of making and uses thereof
US8207363B2 (en) * 2009-03-19 2012-06-26 Martek Biosciences Corporation Thraustochytrids, fatty acid compositions, and methods of making and uses thereof
EP2410996B1 (en) 2009-03-27 2017-08-02 Advanced Bionutrition Corp. Microparticulated vaccines for the oral or nasal vaccination and boostering of animals including fish
CN102459568A (en) 2009-05-26 2012-05-16 先进生物营养公司 Stable dry powder composition comprising biologically active microorganisms and/or bioactive materials and methods of making
CN102656261B (en) 2009-09-18 2015-07-01 菲科尔生物技术国际公司 Microalgae fermentation using controlled illumination
AU2010313249B2 (en) 2009-10-30 2016-05-19 Biojiva Llc Alleviating oxidative stress disorders with PUFA derivatives
CA2785971C (en) 2009-12-28 2019-02-12 Dsm Ip Assets B.V. Production of heterologous polypeptides in microalgae, microalgal extracellular bodies, compositions, and methods of making and uses thereof
EA030095B1 (en) * 2009-12-28 2018-06-29 ДиЭсЭм АйПи АССЕТС Б.В. Production of hemagglutinin-neuraminidase protein in microalgae
WO2011082190A1 (en) 2009-12-28 2011-07-07 Martek Biosciences Corporation Recombinant thraustochytrids that grow on sucrose, and compositions, methods of making, and uses thereof
CN102906270B (en) * 2009-12-28 2016-06-22 Dsmip资产公司 The restructuring thraustochytriale of growth and its compositions, Preparation method and use on xylose
BR112012017831B8 (en) * 2010-01-19 2021-05-25 Dsm Ip Assets Bv microbial oil, animal food and biomass comprising said microbial oil
US9504750B2 (en) 2010-01-28 2016-11-29 Advanced Bionutrition Corporation Stabilizing composition for biological materials
PL2529004T3 (en) 2010-01-28 2017-12-29 Advanced Bionutrition Corporation Dry glassy composition comprising a bioactive material
WO2011112627A1 (en) * 2010-03-11 2011-09-15 BP Biofuels UK Limited Methods, biological oils, biofuels, units, and organisms related to use in compression engines
CN103282474A (en) 2010-04-22 2013-09-04 纳幕尔杜邦公司 Method for obtaining polyunsaturated fatty acid-ontaining compositions from microbial biomass
MX339639B (en) 2010-05-28 2016-06-02 Solazyme Inc * Tailored oils produced from recombinant heterotrophic microorganisms.
JP5911479B2 (en) 2010-06-01 2016-04-27 ディーエスエム アイピー アセッツ ビー．ブイ． Extraction of lipids from cells and products derived therefrom
AU2011262568B2 (en) 2010-06-09 2015-01-29 Fermentalg Compositions comprising eicosapentaenoic acid suitable for high purification
WO2011159682A1 (en) 2010-06-14 2011-12-22 Raveendran Pottathil Methods for the production of algae derived oils
EP2397074B1 (en) 2010-06-19 2012-10-24 M Stenqvist AB A system and computer readable medium for determination of transpulmonary pressure in a patient connected to a breathing apparatus
WO2012021686A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Improved aquaculture meat products
WO2012021703A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company A sustainable aquaculture feeding strategy
US20120040076A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Aquaculture feed compositions
AU2011289272B2 (en) 2010-08-13 2015-02-05 Advanced Bionutrition Corporation Dry storage stabilizing composition for biological materials
US10479969B2 (en) 2010-10-11 2019-11-19 Phycoil Biotechnology International. Inc. Utilization of wastewater for microalgal cultivation
SG10201509035WA (en) 2010-11-03 2015-12-30 Solazyme Inc Microbial Oils With Lowered Pour Points, Dielectric Fluids Produced Therefrom, And Related Methods
MX344012B (en) 2011-02-02 2016-12-02 Terravia Holdings Inc Tailored oils produced from recombinant oleaginous microorganisms.
CN107630017A (en) 2011-03-07 2018-01-26 Dsm营养产品股份公司 It is engineered thraustochytriale microorganism belonging to genus
AU2012249921B2 (en) 2011-04-26 2017-06-08 Biojiva Llc Oxidative retinal diseases
KR102020579B1 (en) 2011-04-26 2019-09-10 레트로토프 인코포레이티드 Impaired energy processing disorders and mitochondrial deficiency
AU2012249917B2 (en) 2011-04-26 2017-06-15 Biojiva Llc Neurodegenerative disorders and muscle diseases implicating PUFAs
CA2834343C (en) 2011-04-26 2021-10-12 Retrotope, Inc. Disorders implicating pufa oxidation
CN103608450A (en) 2011-05-06 2014-02-26 索拉兹米公司 Genetically engineered microorganisms that metabolize xylose
CN102787074B (en) * 2011-05-20 2016-06-08 罗盖特兄弟公司 Produce the Microalgae new strain of squalene
EA027917B1 (en) 2011-07-13 2017-09-29 Оллтек, Инк. Algal lipid compositions and methods of preparing and utilizing the same
MX342216B (en) 2011-07-21 2016-09-20 Dsm Ip Assets Bv Fatty acid compositions.
JP2014532773A (en) 2011-11-01 2014-12-08 ディーエスエム アイピー アセッツ ビー．ブイ． Oils containing oxidatively stable polyunsaturated fatty acids
US9719114B2 (en) 2012-04-18 2017-08-01 Terravia Holdings, Inc. Tailored oils
EP3550025A1 (en) 2012-04-18 2019-10-09 Corbion Biotech, Inc. Tailored oils
WO2014112574A1 (en) 2013-01-18 2014-07-24 協和発酵バイオ株式会社 Microorganisms producing docosahexaenoic acid and utilization thereof
US10098371B2 (en) 2013-01-28 2018-10-16 Solazyme Roquette Nutritionals, LLC Microalgal flour
EP2762008A1 (en) 2013-02-05 2014-08-06 Evonik Industries AG Improving bioavailability of valuable materials from microorganisms by use of a rotor-stator system for cell disruption
EP2953480B1 (en) 2013-02-05 2020-06-03 Evonik Operations GmbH Improving bioavailability of valuable materials from microorganisms
US9873880B2 (en) 2013-03-13 2018-01-23 Dsm Nutritional Products Ag Engineering microorganisms
WO2014176515A2 (en) 2013-04-26 2014-10-30 Solazyme, Inc. Low polyunsaturated fatty acid oils and uses thereof
EP2826384A1 (en) 2013-07-16 2015-01-21 Evonik Industries AG Method for drying biomass
FR3009619B1 (en) 2013-08-07 2017-12-29 Roquette Freres BIOMASS COMPOSITIONS OF MICROALGUES RICH IN PROTEINS OF SENSORY QUALITY OPTIMIZED
AU2014331605A1 (en) 2013-10-04 2016-05-12 Corbion Biotech, Inc. Tailored oils
JP2017501709A (en) 2013-12-20 2017-01-19 ディーエスエム アイピー アセッツ ビー．ブイ． Method for obtaining microbial oil from microbial cells
US11124736B2 (en) 2013-12-20 2021-09-21 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
NZ721417A (en) 2013-12-20 2022-07-01 Dsm Ip Assets Bv Processes for obtaining microbial oil from microbial cells
SG11201605052XA (en) 2013-12-20 2016-07-28 Dsm Ip Assets Bv Processes for obtaining microbial oil from microbial cells
EP3099782B1 (en) 2014-01-28 2019-03-20 DSM IP Assets B.V. Factors for the production and accumulation of polyunsaturated fatty acids (pufas) derived from pufa synthases
CA2941970A1 (en) 2014-03-13 2015-09-17 Retrotope, Inc. Optic neuropathy treatment and reduction of steroid-induced oxidative stress with stabilized polyunsaturated substances
WO2015149026A1 (en) 2014-03-28 2015-10-01 Solazyme, Inc. Lauric ester compositions
FR3019559B1 (en) 2014-04-03 2018-01-05 Fermentalg PROCESS FOR CULTIVATION OF MICROALGUES OF THE GENUS AURANTIOCHYTRIUM IN A SODIUM-CHLORIDE REDUCED CULTURE MEDIUM FOR THE PRODUCTION OF DHA
CN106795539B (en) 2014-05-22 2021-06-22 玛拉可再生能源公司 Method for producing oil in microorganisms
BR112017000414A2 (en) 2014-07-10 2017-11-07 Terravia Holdings Inc ketoacyl acp synthase genes and their uses
JP6402524B2 (en) * 2014-07-30 2018-10-10 富士電機株式会社 Exhaust gas treatment method and exhaust gas treatment apparatus
EP3200604B1 (en) 2014-10-02 2021-11-03 Evonik Operations GmbH Method for preparing an animal feed
CA2958457C (en) 2014-10-02 2022-10-25 Evonik Industries Ag Process for producing a pufa-containing biomass which has high cell stability
CA2958460C (en) * 2014-10-02 2022-09-13 Evonik Industries Ag Process for producing a pufa-containing feedstuff by extruding a pufa-containing biomass
BR112017006833B1 (en) * 2014-10-02 2022-09-13 Evonik Operations Gmbh FOOD FOR ANIMALS CONTAINING POLY-UNSATURATED FATTY ACID AND A PROCESS TO PRODUCE THE SAME
KR101521274B1 (en) 2014-10-08 2015-05-22 에스케이이노베이션 주식회사 Novel microalgae aurantiochytrium sp. la3 (kctc12685bp) and method for preparing bio-oil using thereof
BR112017003558B1 (en) 2014-10-16 2023-10-03 MARA Renewables Corporation REPEAT FED BATCH METHOD OF CULTIVATION OF A MICROORGANISM
EP3207164B1 (en) 2014-10-16 2021-01-20 Mara Renewables Corporation Semi-continuous culture methods
WO2016065231A1 (en) * 2014-10-24 2016-04-28 Trustees Of Dartmouth College Aquaculture feed formulation and aquaculture product produced with the same
US9890402B2 (en) 2015-01-24 2018-02-13 Indian Oil Corporation Limited Thraustochytrid based process for treating waste effluents
FR3031984B1 (en) * 2015-01-27 2019-05-24 Roquette Freres PROCESS FOR ENRICHING THE BIOMASS OF MICROALGUES OF THE GENUS TRAUSTOCHYTRIUM IN DHA AND IN AMINO ACIDS ARG AND GLU
AR104042A1 (en) 2015-03-26 2017-06-21 Mara Renewables Corp HIGH-DENSITY PRODUCTION OF BIOMASS AND OIL USING GLUCEROL IN GROSS
JPWO2017006918A1 (en) 2015-07-03 2018-05-24 国立大学法人九州大学 Microbial oil-producing labyrinthulas, microbial oils, methods for their preparation and their use
JP6977231B2 (en) 2015-07-13 2021-12-08 マラ リニューアブルズ コーポレーション Enhancement of metabolism of C5 organic carbon by microorganisms
FR3038913B1 (en) * 2015-07-17 2020-05-01 Fermentalg THRAUSTOCHYTRIDE BIOMASS, CULTURE METHOD AND USES
AU2016297986B8 (en) 2015-07-29 2020-06-11 Advanced Bionutrition Corp. Stable dry probiotic compositions for special dietary uses
JP2018196327A (en) * 2015-10-14 2018-12-13 味の素株式会社 Sterol production method
CN105334793B (en) * 2015-10-27 2018-05-01 武汉工程大学 A kind of automatic control system and method for preparing artificial seawater
US10730821B2 (en) 2015-11-23 2020-08-04 Retrotope, Inc. Site-specific isotopic labeling of 1,4-diene systems
JP2019068741A (en) * 2016-03-03 2019-05-09 味の素株式会社 Method for producing sterol
US10851395B2 (en) 2016-06-10 2020-12-01 MARA Renewables Corporation Method of making lipids with improved cold flow properties
CN107523504A (en) * 2016-06-21 2017-12-29 嘉必优生物技术（武汉）股份有限公司 Schizochytrium limacinum mutant strain
US11419350B2 (en) 2016-07-01 2022-08-23 Corbion Biotech, Inc. Feed ingredients comprising lysed microbial cells
CA3030471C (en) 2016-07-13 2023-06-20 Evonik Degussa Gmbh Method of separating lipids from a lysed lipids containing biomass
AU2017297752B2 (en) 2016-07-13 2021-09-23 Dsm Ip Assets B.V. Method for isolating lipids from lipid-containing cells
BR112019000435A2 (en) 2016-07-13 2019-04-30 Evonik Degussa Gmbh method for separating lipids from a biomass containing lysed lipids
EP3487970B1 (en) * 2016-07-20 2023-04-05 Mara Renewables Corporation Method of production of flowable crude microbial oil
AU2017301024B2 (en) 2016-07-20 2019-10-17 MARA Renewables Corporation A two-step fractionation method for winterizing oil.
WO2018122057A1 (en) 2016-12-27 2018-07-05 Evonik Degussa Gmbh Method of isolating lipids from a lipids containing biomass
EP3562925B1 (en) 2016-12-27 2021-03-10 Evonik Operations GmbH Method of isolating lipids from a lipids containing biomass
CA3072846A1 (en) 2017-08-17 2019-02-21 Evonik Operations Gmbh Enhanced production of lipids by limitation of at least two limiting nutrient sources
WO2019048327A1 (en) 2017-09-05 2019-03-14 Evonik Degussa Gmbh Method of separating lipids from a lysed lipids containing biomass
EP3470502A1 (en) 2017-10-13 2019-04-17 Evonik Degussa GmbH Method of separating lipids from a lysed lipids containing biomass
EA202090440A1 (en) 2017-09-21 2020-07-17 Эвоник Оперейшенс ГмбХ IMPROVED LIPID PRODUCTION BY LIMITING TWO LIMITING NUTRIENT SOURCES
EP3527664A1 (en) 2018-02-15 2019-08-21 Evonik Degussa GmbH Method of isolating lipids from a lipids containing biomass
WO2019121752A1 (en) 2017-12-20 2019-06-27 Evonik Degussa Gmbh Method of isolating lipids from a lipids containing biomass
WO2019122030A1 (en) 2017-12-22 2019-06-27 Dsm Ip Assets B.V. Method of separating lipids from a lysed lipids containing biomass
WO2019185942A1 (en) 2018-03-29 2019-10-03 Dsm Ip Assets B.V. Use of tocotrienols as antioxidants
WO2019185894A1 (en) 2018-03-29 2019-10-03 Dsm Ip Assets B.V. Novel use of substituted chroman-6-ols
WO2019185941A1 (en) 2018-03-29 2019-10-03 Dsm Ip Assets B.V. Novel use of substituted chroman-6-ols with extended lipophilic side chains
WO2019185910A2 (en) 2018-03-29 2019-10-03 Dsm Ip Assets B.V. Novel use of substituted 2h-chromens and their derivatives
WO2019185891A1 (en) 2018-03-29 2019-10-03 Dsm Ip Assets B.V. Novel use of substituted chroman-6-ols
WO2019185889A1 (en) 2018-03-29 2019-10-03 Dsm Ip Assets B.V. Novel use of carnosic acid
WO2019185940A1 (en) 2018-03-29 2019-10-03 Dsm Ip Assets B.V. Use of twin-chromanols as antioxidants
CN113166090A (en) 2018-03-29 2021-07-23 帝斯曼知识产权资产管理有限公司 Chroman-6-ols having extended lipophilic side chains in position 2, their manufacture and use
WO2019185938A2 (en) 2018-03-29 2019-10-03 Dsm Ip Assets B.V. Novel use of substituted chroman-6-ols with extended lipophilic side chains
WO2019185898A1 (en) 2018-03-29 2019-10-03 Dsm Ip Assets B.V. Novel use of substituted 2h-chromens and their derivatives
WO2019185888A1 (en) 2018-03-29 2019-10-03 Dsm Ip Assets B.V. Novel use of tocopherols
WO2019185939A1 (en) 2018-03-29 2019-10-03 Dsm Ip Assets B.V. Use of twin-chromanols as antioxidants in oil
WO2019219396A1 (en) 2018-05-15 2019-11-21 Evonik Operations Gmbh Method of isolating lipids from a lysed lipids containing biomass by emulsion inversion
WO2019219443A1 (en) 2018-05-15 2019-11-21 Evonik Operations Gmbh Method of isolating lipids from a lipids containing biomass with aid of hydrophobic silica
FR3085825B1 (en) 2018-09-14 2021-07-16 Fermentalg MICROORGANISM OIL RICH IN DOCOSAHEXAENOIC ACID
FR3085962B1 (en) 2018-09-14 2021-06-18 Fermentalg PROCESS FOR EXTRACTING AN OIL RICH IN PUFA
US20220017929A1 (en) * 2018-11-09 2022-01-20 Evonik Operations Gmbh Method for producing a biomass with an increased content of polyunsaturated fatty acids
US20220017930A1 (en) 2018-11-09 2022-01-20 Evonik Operations Gmbh Method for producing a biomass which can be easily broken down and which has an increased content of polyunsaturated fatty acids
CN110330104B (en) * 2019-06-21 2022-04-29 临沂大学 Method for removing p-aminobenzoic acid in water body by using microorganisms
WO2021122770A1 (en) * 2019-12-20 2021-06-24 Dsm Ip Assets B.V. Method of reducing the self-heating propensity of microbial lc-pufa comprising biomass
WO2021168311A1 (en) 2020-02-21 2021-08-26 Retrotope, Inc. Processes for isotopic modification of polyunsaturated fatty acids and derivatives thereof
EP3933016A1 (en) 2020-06-30 2022-01-05 Evonik Operations GmbH Method of isolating lipids from a lipids containing biomass
WO2023144707A1 (en) 2022-01-25 2023-08-03 Dsm Ip Assets B.V. Media refinement and nutrient feeding approaches to increase polyunsaturated fatty acid production
WO2023175141A1 (en) 2022-03-18 2023-09-21 Purac Biochem B.V. Method for reducing fermentation broth viscosity

Family Cites Families (113)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2879162A (en) 1956-10-04 1959-03-24 Du Pont Augmenting poultry feed
US2890989A (en) 1957-07-01 1959-06-16 Ralph F Anderson Method for the production of carotenes
GB857161A (en) 1958-01-07 1960-12-29 Boots Pure Drug Co Ltd Improvements in the propagation of fungi
US3108402A (en) 1961-03-16 1963-10-29 Grain Processing Corp Production of carotenoid pigments
US3444647A (en) 1961-08-08 1969-05-20 Masahito Takahashi Process of cultivating algae
US3142135A (en) 1962-02-13 1964-07-28 Grain Processing Corp Production of carotenoids by the cultivation of algae
US3282794A (en) * 1963-09-19 1966-11-01 Ajinomoto Kk Method of producing citrulline by bacterial fermentation
US3296079A (en) * 1963-12-09 1967-01-03 Pfizer & Co C Products sweetened without sugar and characterized by freedom from aftertaste
US3316674A (en) 1964-09-11 1967-05-02 Yakult Honsha Kk Method of new industrial cultivation of unicellular green algae such as chlorella
GB1143405A (en) 1965-02-25 1969-02-19 Unilever Ltd Processing of foodstuffs and the like
GB1123884A (en) 1966-05-23 1968-08-14 Pfizer & Co C Animal feed compositions
FR1557635A (en) * 1967-04-20 1969-02-21
US3661663A (en) 1968-08-21 1972-05-09 Owens Corning Fiberglass Corp Method of producing siliceous fiber corrosion inhibiting composites
DE1901980A1 (en) * 1969-01-16 1970-09-03 Wunder Kg Heinrich Safety ski binding with swiveling pressure jaws
US3761588A (en) 1969-02-06 1973-09-25 Meiji Seika Kaisha Antibiotics and production thereof
US3617299A (en) 1969-09-15 1971-11-02 Abbott Lab Animal feed premix resistant to static charge and method of making same
US3647482A (en) * 1970-03-04 1972-03-07 Gen Mills Inc Reduction and modification of the unpleasant aftertaste of saccharin
GB1401956A (en) 1971-09-11 1975-08-06 Marmite Ltd Growing of yeast and other microorganisms
DE2219741B1 (en) * 1972-04-21 1973-01-25 Kurt 2000 Hamburg Lohwasser Packaging for food and luxury goods
US3924017A (en) * 1972-07-28 1975-12-02 Gen Foods Corp Sweetness inducer
JPS564233B2 (en) 1973-03-30 1981-01-29
US3908026A (en) * 1973-04-19 1975-09-23 Procter & Gamble Culinary composition containing paramethoxycinnamaldehyde as a flavoring agent and sweetener
US3908028A (en) * 1973-04-19 1975-09-23 Procter & Gamble Soft drink composition containing paramethoxycinnamaldehyde as a flavoring agent and sweetner
GB1466853A (en) 1973-05-22 1977-03-09 Simon Rosedowns Ltd Extraction
US3879890A (en) * 1974-02-06 1975-04-29 Canadian Patents Dev Algal polysaccharide production
US4162324A (en) 1975-11-21 1979-07-24 Merck & Co., Inc. Antibiotics 890A1 and 890A3
IT1109471B (en) 1976-08-17 1985-12-16 Deral Sa PROCEDURE AND PRODUCT FOR THE PRESERVATION AND ENHANCEMENT OF GREEN VEGETABLES AND OF THE WET PRODUCTS UNDER AGRO-FOOD INDUSTRIES
US4229544A (en) 1978-08-08 1980-10-21 Payfer Laboratories Inc. Living organism packaging
US4304794A (en) * 1978-12-14 1981-12-08 Chimicasa Gmbh Artificial-sweetener composition and process of preparing and using same
US4232122A (en) 1979-01-17 1980-11-04 Z-L Limited Partnership Antioxidants, antioxidant compositions and methods of preparing and using same
JPS55111794A (en) 1979-02-19 1980-08-28 Agency Of Ind Science & Technol Production of lipid having high linolic acid content
US4405649A (en) 1979-05-07 1983-09-20 Marvin Dudley Process for producing premium quality fish meal from whole fish
IL57712A (en) 1979-07-03 1984-02-29 Yissum Res Dev Co Cultivation of halophilic algae of the dunaliella species for the production of fuel-like product
DE3011185A1 (en) 1980-03-22 1981-10-01 Kali-Chemie Pharma Gmbh, 3000 Hannover METHOD FOR OBTAINING DIRECTLY APPLICABLE RIN (PARAGRAPH) LECITHIN FOR PHYSIOLOGICAL PURPOSES
GB2098065A (en) 1981-04-14 1982-11-17 Nippon Suisan Kaisha Ltd Antithrombotic compositions containing docosahexaenoic acid
US4426396A (en) 1981-06-11 1984-01-17 Union Oil Company Of California Preservation of harvested crops and animal feedstuffs
IL63734A (en) 1981-09-04 1985-07-31 Yeda Res & Dev Lipid fraction,its preparation and pharmaceutical compositions containing same
WO1983001257A1 (en) 1981-10-07 1983-04-14 Curtain, Cyril, Curtis Method for harvesting algae
US4383038A (en) * 1981-12-10 1983-05-10 Ethyl Corporation Process for the preparation of L-proline by cultivating algae
ATE24266T1 (en) 1982-04-16 1987-01-15 Nestle Sa LIPID COMPOSITION FOR ORAL, ENTERAL OR PARENTERAL NUTRITION.
JPS58196068A (en) * 1982-05-12 1983-11-15 Nec Corp Electrostrictive effect element
JPS58213613A (en) * 1982-06-03 1983-12-12 Sumitomo Bakelite Co Ltd Preparation of spherical active carbon
US4670285A (en) * 1982-08-06 1987-06-02 The University Of Toronto Innovations Foundation Infant formula
US4588600A (en) 1983-04-18 1986-05-13 Scm Corporation Dry premix composition for imparting a fried appearance to baked foods
JPS6087798A (en) * 1983-10-21 1985-05-17 Meiji Milk Prod Co Ltd Production of eicosapentaenoic acid by algae
JPS60105471A (en) * 1983-11-14 1985-06-10 Nisshin Flour Milling Co Ltd Production of health food egg
JPS60133094A (en) 1983-12-21 1985-07-16 日清製油株式会社 Manufacture of high purity eicosapentaenoic acid
EP0155420B1 (en) 1984-02-09 1988-03-23 The Agency of Industrial Science and Technology A method for the preparation of a fungal body and a lipid rich in gamma-linolenic acid therefrom
US4634533A (en) 1985-04-26 1987-01-06 Somerville Robert L Method of converting brines to useful products
US4792418A (en) * 1985-08-14 1988-12-20 Century Laboratories, Inc. Method of extraction and purification of polyunsaturated fatty acids from natural sources
EP0222169B1 (en) * 1985-10-11 1990-08-29 The Nisshin Oil Mills, Ltd. Feedstuff for artemia
US4749522A (en) 1985-10-31 1988-06-07 Angio-Medical Corporation Supercritical fluid extraction of animal derived materials
DE3603000A1 (en) 1986-01-31 1987-08-06 Milupa Ag NEW FATTY MIXTURE OF POLYENIC ACID AND THEIR USE IN THE PRODUCTION OF INFANT FOODS
US4758438A (en) * 1986-10-14 1988-07-19 Stroz John J Sweetener composition
US5023091A (en) 1987-03-23 1991-06-11 The Board Of Regents Of The University Of Nebraska Ruminant feed method of making and method of using
US4957748A (en) 1987-03-23 1990-09-18 The Board Of Regents Of The University Of Nebraska Ruminant feed, method of making and method of using
US5064665A (en) 1987-03-23 1991-11-12 The Board Of Regents Of The University Of Nebraska Method of making and using a ruminant feed
US4764392A (en) 1987-04-01 1988-08-16 Q.P. Corporation Margarine containing fish oil
US4918104A (en) 1987-06-16 1990-04-17 Weiss Howard S Method and composition for increasing the concentration of omega-3 polyunsaturated fatty acids in poultry and poultry eggs and poultry and eggs resulting therefrom
KR890701751A (en) * 1987-07-20 1989-12-21 원본 미기재 Microbial Products of Omega-3 (N-3) Lipids
CA1263270A (en) 1987-08-19 1989-11-28 Bruce J. Holub Animal feed supplement
US4871551A (en) * 1988-02-08 1989-10-03 Microbio Resources, Inc. Pigmentation supplements for animal feed compositions
JPH01218573A (en) 1988-02-25 1989-08-31 Yoshio Tanaka Dunaliella algae-containing solid food and production thereof
US4822500A (en) 1988-02-29 1989-04-18 Texas United Chemical Corporation Saturated brine well treating fluids and additives therefore
US4874629A (en) 1988-05-02 1989-10-17 Chang Stephen S Purification of fish oil
US6451567B1 (en) 1988-09-07 2002-09-17 Omegatech, Inc. Fermentation process for producing long chain omega-3 fatty acids with euryhaline microorganisms
US7033584B2 (en) 1988-09-07 2006-04-25 Omegatech, Inc. Feeding Thraustochytriales to poultry for increasing omega-3 highly unsaturated fatty acids in eggs
US5340594A (en) * 1988-09-07 1994-08-23 Omegatech Inc. Food product having high concentrations of omega-3 highly unsaturated fatty acids
US20060094089A1 (en) 1988-09-07 2006-05-04 Martek Biosciences Corporation Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
US5698244A (en) * 1988-09-07 1997-12-16 Omegatech Inc. Method for raising animals having high concentrations of omega-3 highly unsaturated fatty acids
US5130242A (en) 1988-09-07 1992-07-14 Phycotech, Inc. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
US5340742A (en) * 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US6977167B2 (en) 1988-09-07 2005-12-20 Martek Biosciences Corporation Mixtures of omega-3 and omega-6 highly unsaturated fatty acids from euryhaline microorganisms
US5985348A (en) 1995-06-07 1999-11-16 Omegatech, Inc. Milk products having high concentrations of omega-3 highly unsaturated fatty acids
US5012761A (en) * 1988-11-17 1991-05-07 Oh Suk Y Chicken egg having relatively high percentage of long chain fatty acids and method of reducing heart related disease in humans using such eggs
JP2664452B2 (en) 1988-12-23 1997-10-15 サントリー株式会社 Fish and shellfish feed
DE3920679A1 (en) 1989-06-23 1991-01-10 Milupa Ag FAT MIXTURE FOR THE MANUFACTURE OF FOOD, ESPECIALLY SUGAR FOODS
US5272085A (en) * 1989-10-31 1993-12-21 Queen's University Sodium tolerance genes derived from schizosaccharomyces pombe
US5407957A (en) * 1990-02-13 1995-04-18 Martek Corporation Production of docosahexaenoic acid by dinoflagellates
US5244921A (en) 1990-03-21 1993-09-14 Martek Corporation Eicosapentaenoic acids and methods for their production
US5297625A (en) * 1990-08-24 1994-03-29 Associated Universities, Inc. Biochemically enhanced oil recovery and oil treatment
US5133963A (en) * 1990-12-21 1992-07-28 Shuntaro Ise Method of producing commercially useful poultry products with increased concentrations of Omega-3 polyunsaturated fatty acids
AU661297B2 (en) 1991-01-24 1995-07-20 Martek Corporation Microbial oil mixtures and uses thereof
US5658767A (en) 1991-01-24 1997-08-19 Martek Corporation Arachidonic acid and methods for the production and use thereof
PH11992043811B1 (en) 1991-01-24 2002-08-22 Martek Corp Arachidonic acid and methods for the production and use thereof
KR940005180B1 (en) * 1991-08-20 1994-06-13 주식회사 우방랜드 Feed composition for breeding pigs with n-3 fatty acid-accumulated pork
FR2686619B1 (en) 1992-01-28 1995-07-13 Commissariat Energie Atomique PROCESS FOR THE SELECTIVE PRODUCTION OF POLYUNSATURATED LIPIDS FROM A CULTURE OF PORPHYRIDIUM MICROALGAE AND TANK USED IN THIS PROCESS.
US6410281B1 (en) 1992-07-10 2002-06-25 Omegatech, Inc. Reducing corrosion in a fermentor by providing sodium with a non-chloride sodium salt
JPH078215A (en) * 1993-04-30 1995-01-13 Kawasaki Steel Corp Marine microalgal food raw material containing docosahexaenoic acid and its production
WO1996033263A1 (en) 1995-04-17 1996-10-24 JAPAN, represented by DIRECTOR-GENERAL OF AGENCY OF INDUSTRIAL SCIENCE AND TECHNOLOGY Novel microorganisms capable of producing highly unsaturated fatty acids and process for producing highly unsaturated fatty acids by using the microorganisms
TW486365B (en) 1995-05-30 2002-05-11 Suntory Ltd The process for producing domestic fowl eggs, lipid and food having a high content of highly unsaturated fatty acid
US20080175953A1 (en) 1995-06-07 2008-07-24 Martek Biosciences Corporation Process for the Heterotrophic Production of Microbial Products with High Concentrations of Omega-3 Highly Unsaturated Fatty Acids
US6255505B1 (en) 1996-03-28 2001-07-03 Gist-Brocades, B.V. Microbial polyunsaturated fatty acid containing oil from pasteurised biomass
EP0821068A3 (en) * 1996-03-29 1999-06-02 Rohm And Haas Company Novel sphingolipids and a process thereto
EE04063B1 (en) 1996-07-23 2003-06-16 Nagase Biochemicals, Ltd. Method for the preparation of docosahexaenoic acid and docosapentaenoic acid
PL335227A1 (en) 1997-02-20 2000-04-10 Dsm Nv Industrial-scale production of valuable compounds by fermentation in a chemically defined medium
EP1003869A1 (en) 1997-06-04 2000-05-31 Calgene LLC Production of polyunsaturated fatty acids by expression of polyketide-like synthesis genes in plants
EP1021083B1 (en) * 1997-08-14 2003-05-07 Martek Biosciences Boulder Corporation A method for increasing incorporation efficiency of omega-3 highly unsaturated fatty acid in poultry meat
US5894799A (en) 1997-10-01 1999-04-20 E. I. Du Pont De Nemours And Company Element for cushioning a flexographic printing plate
DE19749413A1 (en) 1997-11-07 1999-05-12 Hoechst Ag Novel sophorose lipids, process for their preparation and use
DE19838011C2 (en) 1998-08-21 2000-01-13 Christoph Syldatk Process for the biotechnical production of fatty acid methyl esters ("biodiesel") based on whey
DE19903095C2 (en) 1999-01-27 2003-05-22 Nutrinova Gmbh Obtaining gamma-linolenic acid from protozoa of the genus Colpidium
JP4252145B2 (en) 1999-02-18 2009-04-08 新日鐵住金ステンレス株式会社 High strength and toughness stainless steel with excellent delayed fracture resistance
WO2000051444A1 (en) 1999-03-04 2000-09-08 Suntory Limited Utilization of material containing docosapentaenoic acid
EP2341126A3 (en) 2000-01-28 2011-10-05 Martek Biosciences Corporation Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors
US6338866B1 (en) 2000-02-15 2002-01-15 Applied Food Biotechnology, Inc. Pet foods using algal or fungal waste containing fatty acids
US6582914B1 (en) 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
TWI426127B (en) 2001-04-16 2014-02-11 Dsm Ip Assets Bv Pufa polyketide synthase systems and uses thereof
MXPA05010214A (en) 2003-03-26 2005-12-14 Martek Biosciences Corp Pufa polyketide synthase systems and uses thereof.
US7208160B2 (en) 2003-08-26 2007-04-24 Sol Katzen Process of treating sea algae and halophytic plants
JP4152861B2 (en) 2003-10-27 2008-09-17 シャープ株式会社 Corner cube reflector, manufacturing method thereof, and reflective display device using the same
DE102004017370A1 (en) 2004-04-08 2005-10-27 Nutrinova Nutrition Specialties & Food Ingredients Gmbh PUFA-PKS Genes from Ulkenia
US8846781B2 (en) * 2007-09-25 2014-09-30 Monsanto Technology Llc Use of oils with high concentrations of polyunsaturated fatty acids in plastics and surface coatings

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9745538B2 (en) 2013-12-20 2017-08-29 MARA Renewables Corporation Methods of recovering oil from microorganisms
US9745539B2 (en) 2013-12-20 2017-08-29 MARA Renewables Corporation Methods of recovering oil from microorganisms
US10745642B2 (en) 2013-12-20 2020-08-18 MARA Renewables Corporation Methods of recovering oil from microorganisms
US11001782B2 (en) 2013-12-20 2021-05-11 Dsm Nutritional Products Ag Methods of recovering oil from microorganisms
US11746363B2 (en) 2013-12-20 2023-09-05 MARA Renewables Corporation Methods of recovering oil from microorganisms

Also Published As

Publication number Publication date
ES2199946T5 (en) 2012-09-07
US5340742A (en) 1994-08-23
JPH11285376A (en) 1999-10-19
ES2199946T3 (en) 2004-03-01
US20060177920A1 (en) 2006-08-10
JPH08502405A (en) 1996-03-19
US20080199923A1 (en) 2008-08-21
EP0669809B1 (en) 2003-06-04
EP1707061A3 (en) 2006-11-15
DE69334309D1 (en) 2010-02-04
DK0669809T3 (en) 2003-09-22
JP4015611B2 (en) 2007-11-28
CA2146235C (en) 2011-12-20
EP0669809A4 (en) 1997-05-21
DE69333026T2 (en) 2004-04-01
EP2179660A2 (en) 2010-04-28
EP2179660A3 (en) 2010-05-19
EP1714561B1 (en) 2009-12-02
US6103225A (en) 2000-08-15
DE69333026D1 (en) 2003-07-10
PT1707061E (en) 2010-03-15
EP1707061B1 (en) 2009-12-23
AU5327194A (en) 1994-05-09
JP4590357B2 (en) 2010-12-01
ATE452541T1 (en) 2010-01-15
US8129172B2 (en) 2012-03-06
US5908622A (en) 1999-06-01
US20070082384A1 (en) 2007-04-12
JP2009232866A (en) 2009-10-15
AU711828B2 (en) 1999-10-21
US8288135B2 (en) 2012-10-16
JP3705950B2 (en) 2005-10-12
EP1298199A3 (en) 2003-12-10
DK1707061T3 (en) 2010-04-26
AU687016B2 (en) 1998-02-19
JP3127161B2 (en) 2001-01-22
US20060160203A1 (en) 2006-07-20
DK1714561T3 (en) 2010-04-12
ES2337384T3 (en) 2010-04-23
ATE241914T1 (en) 2003-06-15
JP4012217B2 (en) 2007-11-21
EP1714561A3 (en) 2006-11-29
WO1994008467A1 (en) 1994-04-28
EP1298199A2 (en) 2003-04-02
JP2005323603A (en) 2005-11-24
EP1714561A2 (en) 2006-10-25
DE69334303D1 (en) 2010-01-14
PT669809E (en) 2003-10-31
DE69333026T3 (en) 2012-10-18
JP2004073215A (en) 2004-03-11
US6566123B1 (en) 2003-05-20
AU4537497A (en) 1998-02-19
ATE450156T1 (en) 2009-12-15
US7381558B2 (en) 2008-06-03
US5518918A (en) 1996-05-21
US20080166780A1 (en) 2008-07-10
ES2338348T3 (en) 2010-05-06
PT1714561E (en) 2010-03-05
EP0669809B2 (en) 2012-05-16
EP0669809A1 (en) 1995-09-06
EP1707061A2 (en) 2006-10-04
US5688500A (en) 1997-11-18
JP2006129875A (en) 2006-05-25

Similar Documents

Publication Publication Date Title
CA2146235A1 (en) Process for growing thraustochytriales
US7022512B2 (en) Schizochytrium and thraustochytrium strains for producing high concentrations of omega-3 highly unsaturated fatty acids
Barclay et al. Heterotrophic production of long chain omega-3 fatty acids utilizing algae and algae-like microorganisms
US5567732A (en) Eicosapentaenoic acid-containing oil and methods for its production
CA2163278A1 (en) Method for production of arachidonic acid
JP2001149087A (en) HETEROTROPHIC PRODUCTION OF MICROBIAL PRODUCT CONTAINING ω-3 HIGHLY UNSATURATED FATTY ACID AT HIGH CONCENTRATION
AU2009213103A1 (en) Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors
US20010000151A1 (en) Eggs containing high concentrations of omega-3 highly unsaturated fatty acids and methods for producing the same
CN104195186A (en) Process for the cultivation of microorganisms of the genus thraustochytriales
JP3536109B2 (en) Foods composed of microorganisms containing high concentration omega-3 polyunsaturated fatty acids
JPH0488954A (en) Method for culturing organism as feed for juvenile fish
KR19980025802A (en) New microorganisms in the genus Erythrobacter
US2798840A (en) Production of vitamin b12 by agrobacterium radiobacter
JPH03119970A (en) Cultivation of organism as feed for fry
AU1351200A (en) A process for growing euryhaline microorganisms

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20131015