CA2195187A1

CA2195187A1 - Enhanced yield blood processing systems and methods establishing controlled vortex flow conditions

Info

Publication number: CA2195187A1
Application number: CA002195187A
Authority: CA
Inventors: Richard I. Brown; Michael R. Prisco
Original assignee: Individual
Current assignee: Baxter International Inc
Priority date: 1995-06-07
Filing date: 1996-05-22
Publication date: 1996-12-19
Also published as: JPH10503712A; EP0775011A4; EP0775011A1; WO1996040404A1; NO970535L; US5628915A; AU6249796A; AU694053B2; NO970535D0

Abstract

Blood processing systems and methods create dynamic vortex flow conditions (130) within a processing chamber (40) by conveying blood into a separation path that extends circumferentially about the rotational axis along an entry path that extends generally parallel to the rotational axis. The vortex flow conditions perfuse blood into the separation path for separation into component parts. The systems and methods confine the vortex flow pattern along the entry path by reducing the radial width of the entry path.

Description

~ WO 96/40404 -- 1 -- 2 1 9 5 1 8 7 pCT~S96,07806 T~UU~ YIELD BLOOD ~ 8Y8TEK8 AND NET~OD8 E8T~RT~ .T.~n VORTEX FLO~

Relate~ A~plications This application is a continuation-in-part of U.S. Patent Application Serial Number 07/814,403 entitled "Centrifuge with Separable Bowl and Spool Elements Providing Access to the Separation Chamber," filed De~ 23, 1991. This application is also a continuation-in-part of U.S. Patent Application Serial Number 07/748,244 entitled "Centrifugation Pheresis System," filed August 21, 1991, which is itself a continuation of U.S. Patent Application Serial No. 07/514,995, filed May 26, 1989, which is itself a continuation of U.S. Patent Application Serial No. 07/009,179, filed January 30, 1987 (now U.S. Patent 4,834,890).

Field of the Invention The invention relates to centrifugal proc~CRing systems and apparatus.
g~ynd oS tho Invention Today blood collection organizations routinely separate whole blood by centrifugation into its various therapeutic , such as red blood cells, platelets, and plasma.
Conventional blood proc~sing systems and methods use durable centrifuge equipment in association with single use, sterile processing chambers, typically made of plastic. The centrifuge equipment introduces whole blood into these chambers while rotating them to create a centrifugal field.
Whole blood separates within the rotating chamber under the influence of the centrifugal field into higher density red blood cells and platelet-rich plasma. An intermediate layer of leukocytes forms an interface between the red blood cells and platelet-rich plasma.
In conventional blood separation systems and methods, platelets lifted into suspension in the PRP can settle back upon the interface. The platelets settle, because the radial velocity of the plasma undergoing separation is not enough to keep the platelets in suspension. Lacking sufficient radial flow, the platelets fall back and settle on the interface. This reduces pror~csing efficiencies, lowering the effective yield of platelets.
- rv of the Invention The invention provides 1 u~_d blood processing systems and methods that create unique dynamic flow conditions within the proc~csi~g chamber.
The systems and methods rotate first and second spaced apart side walls forming a separation zone about a rotational axis. The first wall is closer to the rotational axis than the second wall.
The separation zone defines a separation path having a radial width and which extends generally circumferentially about the axis of rotation. The systems and methods convey blood into the separation zone along an entry path that extend~ generally parallel to the axis of rotation. This establishes a vortex flow pattern in the entry path that ~ w096/40404 _ 3 _ 2 1 9 5 1 8 7 PCT~S96/07806 perfuses blood into the separation path for separation into ~_ -nt parts. ~he systems and methods confine the vortex flow pattern along the entry path to reduce shear stress on the platelets by providing in the first wall a stepped-up ridge along the entry path, thereby reducing the radial width of the entry path.
In a preferred ~ , the systemS and methods direct the perfusion of blood from the stepped-up ridge toward the first wall along a tapered surface that leads to the separation path.
Other features and advantages of the invention will become apparent upon reviewing the following specification, drawings, and ~en~od claims.
Brier DoscriPtion Or the Draw~n~8 Fig. 1 is a side section view of a blood centrifuge having a separation chamber that embodies features of the invention;
Fig. 2 shows the spool element associated with the centrifuge shown in Fig. 1, with an associated proc~ccing container wrapped about it for use;
Fig. 3 i5 a top view of the processing chamber shown in Fig. 2;
Fig. 4A is a pe,D~e~Live view of the centrifuge shown in Fig. 1, with the bowl and spool elements pivoted into their access position;
Fig. 4B is a perspective view of the bowl and spool el~ ~s in their mutually separation condition to allow securing the proc~C~ing container shown in Fig. 2 about the spool element;
Fig. 5 is a peLD~e~Live view of centrifuge shown in Fig. 1, with the bowl and spool el LD
pivoted into their operational position;

wos6/4o4o4 2 l 9 5 1 8 7 PCT~S96107806 -Fig. 6 is an enlarged perspective view of a portion of the processing container shown in Fig.

3 secured to the spool element of the centrifuge, also showing the orientation of the ports serving the interior of the processing chamber and certain surface contours of the spool element;
Fig. 7 iB a somewhat diagrammatic view of the interior of the processing chamber, looking from the low-G wall toward the high-G wall in the region where whole blood enters the proc~Fing chamber for separation into red blood cells and platelet-rich plasma, and where platelet-rich plasma is collected in the processing chamber;
Fig. 8 is a diagrammatic top view of the separation chamber of the centrifuge shown in Fig.
11 laid out to show the radial contours of the high-G and low-G walls;
Fig. 9 is a pe~e~Live interior view of the bowl element, showing the two regions where the high-G wall is not iso-radial;
Figs. 10 to 12 are peL~e~Live exterior views of the spool element, showing the sequential non-iso-radial regions about the circumference Or the low-G wall;
Fig. 13 i5 a top view of the spool element positioned within the bowl element, showing the orientation of the high-G and low-G walls alony the separation chamber;
Figs. 14 to 16 somewhat diagrammatically show a portion of the platelet-rich plasma collection zone in the separation chamber, in which the high-G wall surface forms a tapered wedge for containing and controlling the position of the interface between the red blood cells and platelet-rich plasma;

~ W096i40404 5 2 1 9 5 1 8 7 PCT~S96/07806 Figs. 17 to l9 show the importance of slanting the tapered wedge with respect to the axis of the platelet-rich plasma collection port;
Fig. 20 is a somewhat diagrammatic view of the interior of the processing chamber, looking from the high-G wall toward the low-G wall in the region where platelet-rich plasma begins its 6eparation into platelet ~ enLL~te and platelet-poor plasma, showing the formation of optimal vortex flow pattern for perfusing platelet-rich plasma during separation;
Figs. 21 and 22 are views like Fig. 20, showing the formation of less than optimal vortex flow patterns; and Fig. 23 is a top view of a bowl element and a spool element that embody features of the invention showing radii to major surface regions defined circumferentially on them.
The invention may be Pmho~ied in several forms without departing from its spirit or essential characteristics. The scope of the invention is defined in the ~p~Pn~Pd claims, rather than in the specific description preceding them. All em-bo~i- Ls that fall within the meaning and range of equivalency of the claims are therefore intended to be embraced by the claims.
De~cri~tion of the Pre~erred ~
Fig. l shows a blood centrifuge lO having a blood proceQsing chamber 12 with onh-n~ec1 platelet separation efficiencies. The boundaries of the chamber 12 are formed by a flexible processing container 14 carried within an annular gap 16 between a rotating spool element 18 and bowl element 20. In the illustrated and preferred ~
the processing container 14 takes the form of an 2l 951 87 W096/40404 6 PCT~S96/07806 elongated tube ~see Fig. 3), which is wrapped about the spool element 18 before use, as Fig. 2 shows.
Further details of this centrifuge uu,.~L~uuLion are set forth in U.S. Patent 5,370,802, entitled ~nh~n~ Yield Platelet Systems and Methods,~ which is incorporated herein by reference.
The bowl and spool elements 18 and 20 are pivoted on a yoke 22 between an upright position, as Figs. 4A/4B show, and a ~ 8 position, as Figs.
1 and 5 show.
When upright tsee Fig. 4A), the bowl and spool ~1 18 and 20 are presented for access by the user. A r- ~ ~n;F- permits the spool and bowl elements 18 and 20 to assume a mutually separated position, as Fig. 4B shows. In this position, the spool element 18 is at least partially out of the interior area of the bowl element 20 to expose the exterior spool surface for access. When exposed, the user can wrap the container 14 about the spool element 20 (as Fig. 2 shows). Pins 150 on the spool element 20 (see, e.g., Figs. 6; 10; and 11) engage cutouts on the container 14 to secure the container 14 on the spool element 20.
The - ir~ (not shown) also permits the spool and bowl elements 18 and 20 to assume a mutually ~uu~ ting position, as Fig. 4A shows. In th$s position, the spool element 20 and the secured container 14 are enclosed within the interior area of the bowl element 18.
Further details of the -- ~nia~ for causing relative - ~. L of the spool and bowl elements 18 and 20 as just described are d;arloa~d in U.S. Patent 5,360,542 entitled ~Centrifuge With Separable Bowl and Spool ~1 Ls Providing Acces_ to the Separation Chamber,~ which is incu.~uLated ~ W096/40404 7 2 1 9 5 1 8 7 PCT~S96/07806 herein by reference.
When closed, the spool and bowl elements 18 and 20 can be pivoted into a 5n~p~n~ed position, as Figs. 1 and 5 show. When =u~l~~ l, the bowl and spool elements 18 and 20 are in position for operation.
In operation, the centrifuge 10 rotates the ~ n~l~d bowl and spool ~l~ Ls 18 and 20 about an axis 28, creating a centrifugal field within the processing chamber 12.
The radial boundaries of the centrifugal field (see Fig. 1) are formed by the interior wall 24 of the bowl element 18 and the exterior wall 26 of the spool element 20. The interior bowl wall 24 defines the high-G wall. The exterior spool wall 26 defines the low-G wall.
An umbilicus 30 (see Fig. l) c i~ates with the interior of the procPss;ng container 14 within the centrifugal field and with pumps and other stationary -~ Ls located outside the centrifugal field. A nu.. l~L~ting (zero omega) holder 32 holds the upper portion of the umbilicus 30 in a non-rotating position above the ~ d spool and bowl elements 18 and 20. A holder 34 on the yoke 22 rotates the mid-portion of the umbilicus 30 at a first (one omega) speed about the sll~p~n~
spool and bowl ~1 ~x 18 and 20. Another holder 36 rotates the lower end of the umbilicus 30 at a second speed twice the one omega speed (the two omega speed), at which the u~5~ d spool and bowl ~1- 18 and 20 also rotate. Thi5 known relative rotation of the ~ 'ilic--~ 30 keeps it untwisted, in this way avoiding the need for rotating seals.
As the spool and bowl ~l~ Ls 18 and 20 rotate about the axis 28, blood is in~Luduced into W 096/40404 2 1 9 5 l 8 7 - 8 ~ PCTAUS96/07806 the container 14 through the umbilicus 30. The blood follows a circumferential flow path within the container 14 about the rotational axis 28. When conveying blood, the sidewalls of the container 14 S expand to conform to the profiles of the exterior (low-G) wall 26 of the spool element 18 and the interior (high-G) wall 24 of the bowl element 20.
In the illustrated and preferred ~ ' 'i L
(see Figs. 2 and 3), the processing container 14 iS
divided into two functionally distinct processing LD 38 and 40. More particularly (see Figs.
2 and 3) ~ a first peripheral seal 42 forms the outer edge of the container. A second interior seal 44 extends generally parallel to the rotational axis 28~ dividing the container 14 into the first proc~CRinq compartment 38 and the second processing compartment 40.
Three ports 46/48/50 attached to tubing n~ing from the ~ il'llC 30 icate with the first , i ~ 38. TWO additional ports 52 and 54 attached to tubing extending from the , ~;1;C~'R
30 communicate with the second compartment 40.
As Fig. 6 best shows, the five ports 4 6 to 54 are arranged side-by-side along the top transverse edge of the container 14. When the c~nt ~ i n~r 14 is secured to the spool element 18~ the ports 46 to 54 are all oriented parallel to the axis of rotation 28. The upper region of the exterior wall 26 spool element 18 inr-ln~PR a lip region 56 against which the ports 46 to 54 rest when the ~nn~A i n~r 14 is secured to the spool element 18 for use. Fig. 10 also shows the lip region 56. The lip region 56 extends along an arc of equal radius from the axis of rotation 28. Thus,,all ports 46 to 54 open into the , ; l.zi 38 and 40 at the same ~ W096l40404 2 1 9 5 t 8 7 PCT~Ss6/07806 radial distance from the rotational axis 28.
Each processing compartment 38 and 40 serves a separate and distinct separation function, as will now be described in greater detail.
~e~aration in tha Fir8t Proce85inq Compartment The first compartment 38 receives whole blood tW3) through the port 48. As Fig. 7 best shows, the whole blood separates in the centrifugal field within the first ~ i ~ 38 into red blood cells (RBC, designated by numeral 96), Which move toward the high-G wall 24, and platelet-rich plasma (PRP, designated by numeral 98), which are displaced by v t of the RBC 96 toward the low-G wall 26.
m e port 50 (5ee Figs. 3 and 6) conveys RBC 96 from the first compartment 38, while the port 46 conveys PRP 98 from the first compartment 38.
In the first processing compartment 38, an intermediate layer, called the interface (designed by numeral 58)(see Fig. 7), forms between the RBC 96 and PRP 98. Absent efficient separation conditions, platelets can leave the PRP 98 and settle on the interface 58, thereby lP~cpning the number of platelets in PRP 98 c~l-v~yed by the port 46 from the first c I L 38.
The first compartment 38 (see Figs. 3 and 7) inr~ P~ a third interior seal 60 located between the PRP collection port 48 and the WB inlet port 50.
The third seal 60 inrlll~P~ a first region 62, which is generally parallel to the rotational axis 28.
The third seal also inrllld~P~ a dog-leg portion 64, which bends away from the WB inlet port 48 in the direction of circumferential WB flow in the first _ i ~ 38. The dog-leg portion 64 terminates beneath the inlet of the PRP collection port 48.
The first compartment 38 (see Fig. 3) also 2 1 9 5 1 8 7 PCT~ss6/07806 ~
w096/40404 - 10 -1nr~ a fourth interior seal 66 located between the WB inlet port 48 and the RBC collection port 50.
Similar to the third seal 60, the fourth seal 66 includes a first region 68, which is generally parallel to the rotational axis 28, and a dog-leg portion 70, which bends away from the RBC collection port 52 in the direction of circumferential WB flow in the first compartment 38. The dog-leg portion 70 of the fourth seal 66 extends beneath and beyond the dog-leg portion 64 of the third seal 60. The dog-leg portion 70 terminates near the longitudinal side edge of the first compartment 38 opposite to the longitudinal side edge formed by the second interior seal 44.
Together, the third and fourth interior seals 60 and 66 form a WB inlet passage 72 that first extends along the axis of rotation and then bends to open in the direction of intended circumferential flow within the first compartment 38, there defining a WB entry region 74, of which Fig. 7 shows an interior view). The third interior seal 60 also forms a PRP collection region 76 within the first I ' 38, of which Fig. 7 also ~hows an interior view.
As Fig. 7 best shows, the WB entry region 74 i8 next to the PRP collection region 76. This close juxLaposition creates dynamic flow conditions that sweep platelets into the PRP collection region 76.
More particularly, the velocity at which the RBC 96 settle toward the high-G wall 24 in ~e~u..~e to centrifugal force is greatest in the WB
entry region 74 than ~l~e~h~re in the first ~ ; ' 38. Further details of the distribution of RBC 96 during centrifugation in a chamber are set ~ w096/40404 ~ 21 9 5 1 8 7 pCT~S96/07806 forth in Brown, "The Physics Or Continuous Flow Centrifugal Cell Separation," Artificial orqans.
~ 13(1):4-20 (1989).
There is also relatively more plasma volume ~ 5 to displace toward the low-G wall 26 in the WB entry region 74. As a result, relatively large radial plasma velocities toward the low-G wall 26 occur in the WB entry region 74. These large radial velocities toward the low-G wall 26 elute large numbers of platelets from the RBC 96 into the close-by PRP collection region 76.
Together, the fourth interior seal 66, the second interior seal 44, and the lower regions of the first peripheral seal 42 form a RBC collection passage 78 (see Fig. 3). The RBC collection passage 78 extends first along the axis of rotation 28 and then bends in a circumferential path to open near the end of the intended WB circumferential flow path, which comprises a RBC collection region 80.
As Fig. 8 shows, the contoured surrace of the exterior wall 26 of the spool element 18 bol~n~;n7 the low-G side of the first compartment 38 cnntln~tnl~ly changes in terms of its radial distance from the rotational axis 28. At no time does the exterior (low-G) wall 26 of the spool element 18 comprise an iso-radial contour with respect to the rotational axis 28. On the other hand, the surface of the interior (high-G) wall 24 of the bowl element 20 bo~n~ing the high-G side of the first compartment is iso-radial with respect to the rotational axis 28, except for two 1OCA1;7ed~ axially aligned regions in the first ~ nt 38, where the radial contours change. The juxtaposition of these co.,-~uL~d surfaces on the exterior (low-G) wall 26 of the spool element 18 and the interior (high-G) W 096l40404 21 95:1 87 P~r~US96/07806 -wall of the bowl element 20 bol~n~linq the first compartment 38 further enhance the separation conditions that the interior structure of the t, ~~ L 38 create.
More particularly, the juxtaposed surface contours of the high-G and low-G walls 24 and 26 create a first dynamic flow zone 82 in the PRP
collection region 76 of the first compartment 38.
There, the contour of the high-& wall 24 forms a tapered wedge (see Fig. 9) comprising first and second tapered surfaces 84 and 86. These surfaces 24 project from the high-G wall 24 toward the low-G
w all 26. The slope of the first tapered surface 84 is less than the slope of the second tapered surface 86; that is, the second tapered surface 86 is steeper in pitch than the first tapered surface 84.
Radially across from the tapered surfaces 84 and 86, the contour of the low-G exterior wall 26 of the spool element 18 forms a flat surface 88 (see Figs. 10 and 13) . In terms of its radial .li- -ion~
(whLch Fig. 8 shows), the flat surface 88 first decreases and then increases in radius in the direction Or W8 flow in the first compartment 38.
The flat surface 88 thereby presents a decrease and then an increase in the centrifugal field along the low-G wall 26. The flat surface 88 provides clearance for the first and second tapered surfaces 84 and 86 to nc '~te ~ ~ L of the spool and bow l el~ -- ts 18 and 20 between their mutually separated and mutually cooperating positions. The flat surface 88 al80 creates a second dynamic flow zone 104 in cc,~ .c.tion with a flat surface 106 facing it on the high-G wall 24 in the WB entry region 74 (see Fig. 9), as will be described in greater detail later.

~ WO 96/40404 PCT/US96/07806 As Figs. 14 to 16 show, the facing first surface 84 and flat surface 88 in the first zone 82 form a constricted passage 90 along the low-G wall 26, along which the PRP 98 layer extends. As shown diagrammatically in Fiqs. 14 to 16, the tapered surface 86 diverts the fluid flow along the high-G
wall 24 of the first compartment 38, keeping the interface 58 and RBC 96 away from the PRP collection port 46, while allowing PRP 98 to reach the PRP
collection port 46.
This flow diversion also changes the orientation of the interface 58 within the PRP
collection region 76. The second tapered surface 86 displays the interface 26 for viewing through a side wall of the container by an associated interface controller (not shown). Further details of a preferred ~ '-'ir--t for the interface controller 134 are described in U.S. Patent 5,316,667, which is incuL~uLated herein by reference.
The interface controller monitors the location of the interface 58 on the tapered surface 86. As Figs. 14 to 16 show, the position of the interface 58 upon the tapered surface 86 can be altered by controlling the relative flow rates of WB, the Ri3C 96, and the PRP through their respective ports 48, 50, and 46. The controller 134 varies the rate at which PRP 98 is drawn from the first compartment 38 to keep the interface 58 at a prescribed preferred location on the tapered surface 86 (which Fig. 15 shows), away rrom the constricted passage 90 that lead~ to the PRP collection port 46.
Alternatively, or in combination, the controller 134 could control the location of the interface 58 by varying the rate at which WB is introduced into the fir~t --; ' 38, or the rate at which RBC are .

~1 951 87 W096/40404 - 14 - PCT~S96/~7806 -conveyed from the first compartment 134, or both.
In the illustrated and preferred ~
(see Figs. 17 to 19~, the major axis 94 of the tapered surface 86 is oriented at a non-parallel angle ~ with respect to the axis 92 of the PRP port 46. The angle ~ is greater than 0~ (i.e., when the surface axis 94 is parallel to the port axis 92, as Fig. 17 shows), but is preferably less than about 45~, as Fig. l9 shows. Most preferably, the angle is about 30~.
When the angle ~ is at or near 0~ (see Fig.
17), the buulld~Ly of the interface 58 between RBC 96 and PRP 98 is not uniform along the tapered surface 86. Instead, the boundary of the interface 58 bulges toward the tapered surface 84 along the region of the surface 86 distant to the port 46. RBC
96 spill into the constricted passage 90 and into the PRP 98 exiting the PRP port 46.
When the angle a is at or near 45~ (see Fig.
19), the Lu~l~d~-y of the interface 58 between RBC 96 and PRP 98 is also not uniform along the tapered surface 86. Instead, the boundary of the interface 58 bulges toward the tapered surface 84 along the region of the surface 86 close to the port 46. RBC
96 again spill into constricted passage 90 and into the PRP 98 exiting the PRP port 46.
As Fig. 18 shows, by presenting the desired angle ~, the collected PRP 98 is kept ~qq~ti~lly free of RBC 96 and leukocytes.
The juxtaposed surface UU~I~U~LD of the high-G and low-G walls 24 and 26 further create a second dynamic flow zone 104 in the WB entry region 74 Or the first compartment 38. There, the contour of the high-G wall 24 forms a flat surface 106 (see Fig. 9) spaced along the rotational axis 28 below ~ w096/40404 - 15 - PCT~Sg6/07806 the tapered surfaces 84 and 86. The flat surface 106 also faces the already described flat surface 88 on the low-G wall 26 (see Fig, 13). In terms of its radial ~ cionq (which Fig. 8 shows), the flat surface 106 on the high-G wall 24 first decreases and then increases in radius in the direction of NB
flow in the first compartment 38. The flat surface 106 thereby ~Lesel.Ls a decrease and then an incFease in the centrifugal field along the high-G wall 24.
10The boundaries of the first and second zones 82 and 104 are generally aligned in an axial direction with each other on the high-G wall 24 (see Fig. 7), as well as radially aligned with the boundaries of the flat surface 88 on the low-G wall 1526 (see Fig. 13). The first and second zones 82 and 104 therefore circumferentially overlap in a spaced rela~in~qh1p along the axis of rotation 28 in the first compartment 38.
m is ju~L~position of the two zones 82 and 104 Dnh~nrPq the dynamic flow conditions in both the WB entry region 74 and PRP collection region 76.
The radially opposite flat surfaces 88 and 106 of the second zone 104 form a flow-restricting dam on the high-G wall 24 of the NB entry region 74. Flow of WB in the WB inlet passage 72 is generally conrused and not uniform (as Fig. 7 shows). The zone dam 104 in the NB entry region 74 restricts WB
flow to a reduced passage 108, thereby causing more uniform perfusion of NB into the first 38 along the low-G wall 26.
The ju~L~po~ition of the first and second zones 82 and 104 places this uniform perfusion of N~3 adjacent to the PRP collection region 76 and in a plane that is approximately the same as the plane in which the preferred, controlled position of the W096/40404 2 1 9 5 1 8 7 PCT~596/07806 -interface 58 lies. Once beyond the constricted passage 108 of the zone dam 104, the RBC 96 rapidly move toward the high-G wall 24 in rea~v..se to centrifugal force.
The constricted passage 108 of the zone dam 104 brings WB into the entry region 74 at approximately the preferred, controlled height of the interface 58. WB brought into the entry region 74 below or above the controlled height of the interface 58 will immediately seek the interface height and, in so doing, oscillate about it, causing e~ secu.,daLy flows and p_LLu~L~Lions along the interface 58. 8y bringing the WB into the entry region 74 approximately at interface level, the sone dam 104 reduces the lncid~n~e of secondary flows and peLLu~bations along the interface 58.
The juxLaposed surface contours of the high-G and low-G walls 24 and 26 further create a third dynamic flow zone 110 beyond the WB entry region 74 and the PRP collection region 76 of the first compartment 38. There (~ee Figs. 8, 10 and 11), the surface 111 of the low-G wall 26 tapers outward away fro~ the axis of rotation 28 toward the high-G wall 24 in the direction of WB flow. In this zone 110, the high-G wall surface 113 across from the surface 111 retains a constant radius.
This juxtaposition of contours along the high-G and low-G walls 24 and 26 produces a dynamic c$rcumferential plasma flow condition generally transverse the centrifugal force field in the direction of the PRP collection region 76. The circumferential plasma flow condition in this direction con~in~u~ly drags the interface 58 back toward the PRP collection region 76, where the higher radial plasma flow conditions already ~ w096/40404 - 17 - 2 1 9 5 1 8 7 PCT~Sg6/07806 described exist to sweep even more platelets off the interface 58. Simultaneously, the counterflow patterns serve to circulate the other heavier ~_ ts of the interface 58 (the lymphocytes, monocytes, and granulocytes) back into the RBC mass, away from the PRP 98 stream.
The juxtaposed surface contours of the high-G and low-G walls 24 and 26 further create a fourth dynamic flow zone 112 in the RBC collection region 80 of the first compartment 38. There, the surface 115 Or the low-G wall 26 steps radially toward the high-G wall 24, while the high-G wall 24 remains iso-radial. This Ju~L~osition Or the high-G
and low-G walls 24 and 26 creates a stepped-up barrier zone 112 in the RBC collection region 80.
The ste~ed ~y barrier zone 112 extends into the RBC
mass along the high-G wall 24, creating a restricted passage 114 between it and the facing, iso-radial high-G wall 24 (see Fig. 8). The restricted passage 114 allows RBC 96 present along the high-G wall 24 to move beyond the barrier zone 112 for colleC~i~n by the RBC collection passage 78. Simultaneously, the stepped-up barrier zone 112 blocks the passage of the PRP 98 beyond it, keeping the PRP 98 within the dynamic flow conditions created by the first, second, and third zones 82, 104, and 110.
As Fig. 3 shows, the dog leg portion 70 of the RBC collection passage 78 is also tapered. Due to the taper, the passage 78 p~.s_.-L~ a greater cross section in the RBC collection region 80. The taper of the dog leg portion 70 is preferably gauged relative to the taper of the low-G wall 26 in the third flow zone 110 to keep fluid resistance within the passage 78 relatively ~ allL~ while maximizing the available separation and collection areas w096i40404 2 1 9 5 1 8 7 -- 18 -- PCT/US96107806 outside the passage 78. The taper of the dog leg portion 70 also facilitates the removal of air from the pacsage 78 during priming.
8eparation in tho 8econd PLoc~sin~ Compartment The second processing compartment 40 receives PRP 98 from the first processing , i L 38 through the port 56 (of which Fig. 20 shows an interior view). The PRP 98 separates in the centrifugal field within the second compartment 10 40 into platelet ~ol.c~L ~te (PC, designated by numeral 116J, which moves toward the high-G wall 24, and platelet-poor plasma (PPP, designated by numeral 118J, which is displaced by the moving PC toward the low-G wall 26. The port 54 conveys PPP 118 from the second compartment 40. The PC 116 remains in the second ~ -I L 40 for later ~ ion and transport to an external storage container.
The ~econd compartment 40 (see Fig. 3) includes a fifth interior seal 120 between the PRP
inlet port 56 and the PPP collecti~n port 54. The fifth seal 120 extends in a first region 122 generally parallel to the second seal 44 and then bends away in a dog-leg 124 in the direction of circumferential PRP flow within the second , ; L 40. The dog-leg portion 124 terminates near the longitudinal cide edge of the second compartment 40 opposite to the longitudinal side edge formed by the second interior seal 90.
The fifth interior seal 120, the second interior seal 90, and the lower regions of the first peripheral seal 42 together form a PPP collection passage 126. The PPP coll~c~;~n passage 1126 receives PPP at its open end and from there c~nn~lc the PPP to the PPP collection port 54.
PRP enters the second compartment 40 in a ~ W096/40404 19 PCT~S96/07806 PRP entry region 128 (5ee Fig. 20). The PRP enters the region 128 through the port 56 in an axial path.
The PRP departs the region 128 in a circumferential path toward the opposite longitudinal side edge.
This create~ within the PRP entry region 128 a vortex flow pattern 130 (see Fig. 20), called a Taylor column. The vortex flow pattern 130 circulates about an axis 132 that is gene~ally parallel to the rotational axis 28 and stretches from the outlet of the port 56 longit~l~;nAlly across the ciL~u."f~.,Lial flow path of the chamber 40. The vortex region flow pattern 130 perfuses the PPP into the desired circumferential flow path for separation into PC 116 and PPP 118 in a sixth flow zone 140 located beyond the PRP entry region 128.
In the illustrated and preferred embodiment, the surface of the low-G wall 26 i8 cv.lLvuLed to create a flfth dynamic flow zone 134 in the PRP entry region 128. The flow zone 134 controls the perfusion effects of the vortex flow pattern 130.
Nore particularly, in the fifth flow zone 134, the surface of the low-G wall 26 steps radially toward the high-G wall 24 to form a stepped-up ridge 136 in the PRP entry region 128 (see Figs. 8; 13;
and 20). In the fifth flow zone 134, the low-G wall then radially recedes away from the high-G wall 24 to form a tapered surface 138 leading from the ridge 136 in the direction of circumferential PRP flow.
The high-G wall 24 remains iso-radial U.~ vu~hvuL the fi~th flow zone 134, and the 1~ ln~P~ of the second ,- L L 40 The stepped up ridge 136 reduces the radial width of the PRP entry region 128. The reduced radial width reduces the DL~ yU~ of the vortex flow WO 96/4041~4 PCT/US96/07806 pattern 130, thus lowering the shear rate and subsequent shear stress on the platelets. The reduced radial width also reduces the time that platelets dwell in the vortex flow pattern 130. sy both reducing shear stress and exposure time to such shear stress, the reduced radial width reduces the 1 ;k~l ;hnod of damage to platelets.
The reduced radial width also creates a vortex flow pattern 130 that is more confined, compared to the flow pattern 130' with a less radially confined area, as Fig. 21 shows. The trailing tapered surface 138 also further directs the perfusion of PRP gently from the more confined vortex flow pattern 130 toward the low-G wall 26 and into the sixth flow zone 140. The results are a more effective separation of PC from the PRP in the sixth flow zone 140.
The sixth flow zone 140 has a greater radial width than the PRP entry region 128. This greater radial width is desirable, because it provides greater volume for actual separation to occur.
The radial width of the PRP entry region 128 i8 believed to be important to optimize the benefits of the vortex flow pattern 130 in separating PC from PRP. If the radial width i8 too large (as shown in Fig. 21), the resulting vortex flow pattern 130' is not well confined and more vigorous. Platelets are held longer in the flow pattern 130, while also being subjected to higher shear stress.
On the other hand, if the radial width of the PRP entry region 128 is too small tas Fig. 22 shows), the increasing flow resistance, which increases in cubic fashion as the radial width ~ W096/40404 - 21 - PCT~S96/07806 decreases, will cause the vortex pattern 130 to shift out of the region of small radial width into a region where a larger radial width and less flow resistance exists. Thus, the vortex flow pattern will not occur in the PRP entry region 128. Instead, the flow pattern 130" will form away from axial alignment with the PRP port 56, where a larger radial width, better conducive to vortex flow, is present. The effective length of the circumferential separation path is shortened, leading to reduce separation effici~nci~.
Furthermore, the resulting, shifted vortex flow pattern 130 is likely not to be well confined and will thus subject the platelets to undesired shear ~r~sses and dwell time.
The ~ i nnl ~ pal t ~ (~) can be used to differentiate between a radial width that is too wide to provide well confined control of the vortex flow pattern 130 and reduced width that does.
Di~cl~ed in U.S. Patent 5,316,667, the dimensionless pa. Dr (A) accurately characterizes the ' in~ attributes of angular velocity, channel ;r~nP~ or radial width, kinematic viscosity, and axial height of the channel, e~Le4sed as follows:

(2nh 3) ('~Z) where:
n is the angular velocity (in rad/sec);
h is the radial depth (or ~h;c~n~s) of the chamber tin cm);
u is the kinematic viscosity of the fluid being separated (in cm2/sec); and W 0 96/40404 2 1 ~5 ~ 8 7- 22 - P~r~JS96/07806 Z i5 the axial height of the chamber (in cm).
It is believed that a reduced radial width in the PRP entry region 128 sufficient to provide a parameter (~) < 100 will promote the desired confined vortex flow conditions shown in Fig. 20.
A paL ' Pr (~) of about 40 to 50 is preferred. Due to a larger radial width in the sixth flow zone 140 (realizing that the angular velocity and the kinematic viscosity of the PRP being separated remain essentially the same) the parameter (~) will be significantly larger in the sixth flow zone 140.
Parameters (~) typically can be DYrected in the sixth flow zone 140 to be in the neighborhood of 500 and more.
It is believed that flow resistance, expressed as the change in pLes~uLe per unit flow rate, can be used to define the boundary at which a narrower radial width in the PRP entry region 128 causes shifting of the vortex flow pattern 130, as Fig. 22 shows. Empirical evidence ~u~ L~ that vortex flow shifting will occur in the region 128 when flow resistance in the vortex reaches about go dyne sec/cm4, which i8 equivalent to the flow rD~t~nre plasma encounters flowing at 30 ml/min in a space that is o.l cm wide, 1.0 cm long, and 5.0 cm high, while being rotated at 3280 RPM.
~ he juxtaposed surface contours of the high-G and low-G walls 24 and 26 further create the sixth dynamic flow zone 140 beyond the PRP entry region 128 of the second _ --L L 40. Here, the surface 141 of the low-G wall 26 tapers outward awAy from the axis of rotation 28 toward the high-G wall 24 in the direction of p~fused PRP flow in the second ; 40. In this zone 140, the high-G

21 95~ 87 ~ W096/40404 - 23 - PCT~S96/07806 wall 24 retains a constant radius.
The tapered low-G wall 26 in the sixth flow zone 140 provides a greater radial width where a substantial majority of PC 5eparation occurs.
Typically, most of PC separation occurs in the first half segment of the sixth flow zone 140. The PC
deposit along the high-G wall 24 in great amounts in this half 6.-, L of the sixth flow zone 140, creating a layer along the high-G wall 24 in this half-segment as much as 1 mm in thickness. The greater radial width in this half s~ ~ of the sixth flow zone Al '-tes the c~ e..tL~ted volume of PC without adversely reducing the nP~egSAry separation volume.
In the illustrated and preferred ~mho~i- t, the dog-leg portion 124 of the associated PPP collection passage 126 is tapered.
As with the taper of the dog leg portion 70, the taper of the dog-leg portion 124 is preferably gauged relative to the taper of the low-G
wall 26 to keep fluid resistance within the PPP
collection passage 126 relatively constant. The taper also facilitates the removal of air from the passage 126 during priming.
As Figs. 8 and 10 best shows, the surface 142 of the low-G wall 26 of the spool element 18 between the first flow zone 82 (in the first t L 38) and the fifth flow zone 134 (in the ~econd ~ ; ~ 40) tapers away from the high-G
wall 24 in the direction from the fifth zone 134 toward the first zone 82. The radial facing surface of the high-G wall 24 remains iso-radial. The portion of the PPP collection passage 126 axially aligned with the PPP collection port 54 (in the second compartment 40) and the portion of the RBC

W096l40404 - 24 - PCT~596/07806 -collection passage 78 axially aligned with the RBC
collection port 52 (in the first -~ ~ 38) are carried between this low-G surfaces 142 and the opposed high-G wall. The surface 142 provides a smooth transition between the PRP entry region 128 and the WB entry region 74.
Fig. 23 shows radii A to G for the principal surface regions described above along the spool element 18 and the bowl element 20. The following table lists the dimensions of these radii in a preferred implementation:
Radii Dimension (inches) A 0.035 B 3.062 C 3.068 D 2.979 E 3.164 F 3.070 G 2.969 The axial height of the surfaces in the preferred implementation is 3.203 inches.
In a preferred implementation (see Fig.
14), the surface 84 projects from the high-G wall for a distance (d~ ion H in Fig. 14) of .268 inch. The circumferential length of the surface 84 (di- -ion I in Fig. 14) is .938 inch, and the length of the tapered surface 86 (~i -ion J in Fig. 14) i8 . 343 inch. The angle of the tapered surface 86 is 29 degrees.
In a preferred implementation (see Flg. 9), the surface 106 projects from the high-G wall for a distance (~i- ~ion K in Fig. 9) of .103 inch. The ~ WO 96/40404 -- 2 5 -- 2 1 q 5~1 8 7 PCT/US96/07806 circumferential length o~ the sur~ace 106 (dimension L in Fig. 9) is 1.502 inches.
Various features of the inventions are set forth in the following claims.

Claims

I claim:

1. A chamber for rotation about a rotational axis to separate blood components comprising first and second spaced apart side walls forming a separation zone, the first wall being closer to the rotational axis than the second wall, the separation zone defining a separation path having a radial width, the separation path extending generally circumferentially about the axis of rotation, an inlet to convey blood into the separation zone along an entry path that extends generally parallel to the axis of rotation, the blood moving in a vortex flow pattern in the entry path for perfusion into the separation path and separation into component parts, and the first wall including a stepped-up ridge along the entry path to establish along the entry path a radial width less than the radial width of the separation path to thereby confine the vortex flow pattern.

2. A chamber according to claim 1 wherein the first wall further includes a tapered surface leading from the stepped up ridge in the direction of perfusion of blood to direct the perfusion toward the first wall and into the separation path.

3. A chamber according to claim 1 or 2 wherein the second wall is iso-radial in the entry path.

4. A chamber according to claim 1 or 2 wherein the second wall is iso-radial in the separation path.

5. A chamber according to claim 4 wherein the second wall is iso-radial in the entry path.

6. A chamber according to claim 1 and further including an outlet to convey at least one of the separated component parts from the separation path along an exit path that extends generally parallel to the axis of rotation.

7. A method for separating blood components comprising the steps of rotating first and second spaced apart side walls forming a separation zone about a rotational axis, the first wall being closer to the rotational axis than the second wall, the separation zone defining a separation path having a radial width, the separation path extending generally circumferentially about the axis of rotation, conveying blood into the separation zone along an entry path that extends generally parallel to the axis of rotation to establish a vortex flow pattern in the entry path that perfuses blood into the separation path for separation into component parts, confining the vortex flow pattern along the entry path by providing in the first wall a stepped-up ridge along the entry path, thereby reducing the radial width of the entry path.

8. A method according to claim 7 and further including the step of directing the perfusion of blood from the stepped-up ridge toward the first wall along a tapered surface that leads to the separation path.

9. A method according to claim 7 and further including the step of conveying at least one of the separated component parts from the separation path along an exit path that extends generally parallel to the axis of rotation.