CA2209080A1 - Fluorometric assay for detecting nucleic acid cleavage - Google Patents

Fluorometric assay for detecting nucleic acid cleavage

Info

Publication number
CA2209080A1
CA2209080A1 CA002209080A CA2209080A CA2209080A1 CA 2209080 A1 CA2209080 A1 CA 2209080A1 CA 002209080 A CA002209080 A CA 002209080A CA 2209080 A CA2209080 A CA 2209080A CA 2209080 A1 CA2209080 A1 CA 2209080A1
Authority
CA
Canada
Prior art keywords
fluorometric assay
nucleic acid
detecting nucleic
acid cleavage
dna cleavage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002209080A
Other languages
French (fr)
Other versions
CA2209080C (en
Inventor
Myun Ki Han
S. Paul Lee
Jack G. Chirikjian
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Georgetown University
Original Assignee
Georgetown University
Myun Ki Han
S. Paul Lee
Jack G. Chirikjian
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Georgetown University, Myun Ki Han, S. Paul Lee, Jack G. Chirikjian filed Critical Georgetown University
Publication of CA2209080A1 publication Critical patent/CA2209080A1/en
Application granted granted Critical
Publication of CA2209080C publication Critical patent/CA2209080C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method of detecting an enzyme-mediated DNA cleavage reaction in a fluorometric assay is provided. The method can be used to detect DNA cleavage caused by restriction endonucleases, retroviral integrase enzymes, DNases, RNases, or enzymes utilized in other strand separating processes in molecular biology.
CA002209080A 1994-12-30 1995-12-27 Fluorometric assay for detecting nucleic acid cleavage Expired - Fee Related CA2209080C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US36547394A 1994-12-30 1994-12-30
US08/365,473 1994-12-30
PCT/US1995/016904 WO1996021144A1 (en) 1994-12-30 1995-12-27 Fluorometric assay for detecting nucleic acid cleavage

Publications (2)

Publication Number Publication Date
CA2209080A1 true CA2209080A1 (en) 1996-07-11
CA2209080C CA2209080C (en) 2008-07-29

Family

ID=23439046

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002209080A Expired - Fee Related CA2209080C (en) 1994-12-30 1995-12-27 Fluorometric assay for detecting nucleic acid cleavage

Country Status (5)

Country Link
US (1) US5763181A (en)
EP (1) EP0803058B1 (en)
CA (1) CA2209080C (en)
DE (1) DE69535882D1 (en)
WO (1) WO1996021144A1 (en)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6821727B1 (en) 1993-11-15 2004-11-23 Applera Corporation Hybridization assay using self-quenching fluorescence probe
US5547861A (en) 1994-04-18 1996-08-20 Becton, Dickinson And Company Detection of nucleic acid amplification
US6787304B1 (en) 1994-12-28 2004-09-07 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US20030165908A1 (en) * 1994-12-30 2003-09-04 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US6459805B1 (en) * 1996-03-26 2002-10-01 Childrens Hospital Los Angeles Fluorescence digital imaging microscopy system
US5846726A (en) * 1997-05-13 1998-12-08 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US5928869A (en) * 1997-05-30 1999-07-27 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US5935791A (en) 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
GB9725237D0 (en) * 1997-11-29 1998-01-28 Secr Defence Amplification system
ATE469982T1 (en) * 1998-02-04 2010-06-15 Life Technologies Corp DETERMINATION OF THE GENOTYPE OF AN AMPLIFICATION PRODUCT AT MULTIPLE ALLELE SITES
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
US6391551B1 (en) 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
AU765428B2 (en) * 1998-03-13 2003-09-18 Promega Corporation Methods for determining the presence of nucleic acid target sequences and applications thereof
US7090975B2 (en) 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
US6268146B1 (en) 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6270974B1 (en) 1998-03-13 2001-08-07 Promega Corporation Exogenous nucleic acid detection
US6270973B1 (en) 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
US6335162B1 (en) 1998-03-13 2002-01-01 Promega Corporation Nucleic acid detection
US6312902B1 (en) 1998-03-13 2001-11-06 Promega Corporation Nucleic acid detection
US6277578B1 (en) * 1998-03-13 2001-08-21 Promega Corporation Deploymerization method for nucleic acid detection of an amplified nucleic acid target
US6703211B1 (en) 1998-03-13 2004-03-09 Promega Corporation Cellular detection by providing high energy phosphate donor other than ADP to produce ATP
AU774085B2 (en) * 1999-02-18 2004-06-17 Promega Corporation Multiplex method for nucleic acid detection
DE60045059D1 (en) * 1999-04-20 2010-11-18 Nat Inst Of Advanced Ind Scien Methods and probes for determining the concentration of nucleic acid molecules and methods for analyzing the data obtained
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6844154B2 (en) 2000-04-04 2005-01-18 Polygenyx, Inc. High throughput methods for haplotyping
US6323337B1 (en) 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
AU2001270098A1 (en) * 2000-06-22 2002-01-02 San Diego State University Foundation Recombination modulators and methods for their production and use
WO2002042497A2 (en) * 2000-11-27 2002-05-30 Memorial Sloan-Kettering Cancer Center Methods using fluorescens energy transfer probes for detecting cleavage of nucleic acids
CA2448097A1 (en) 2001-05-22 2002-11-28 University Of Chicago N4 virion single-stranded dna dependent rna polymerase
US7172865B2 (en) * 2001-08-13 2007-02-06 Saint Louis University Rapid and sensitive assay for the detection and quantification of coregulators of nucleic acid binding factors
US6544746B2 (en) * 2001-08-13 2003-04-08 St. Louis University Rapid and sensitive proximity-based assay for the detection and quantification of DNA binding proteins
AU2003208959A1 (en) 2002-01-30 2003-09-02 Id Biomedical Corporation Methods for detecting vancomycin-resistant microorganisms and compositions therefor
GB0205455D0 (en) * 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
FR2836926A1 (en) * 2002-06-14 2003-09-12 Commissariat Energie Atomique New oligonucleotide substrate, useful for detecting DNA repair activity, includes double strand region containing a lesion and linked to an energy transfer pair
WO2004055198A2 (en) * 2002-12-12 2004-07-01 Chiron Corporation Device and method for in-line blood testing using biochips
KR101041106B1 (en) * 2003-11-25 2011-06-13 한면기 Novel realtime detection of nucleic acids and proteins
US9012142B2 (en) * 2005-02-15 2015-04-21 Georgetown University Sequence-specific detection of nucleotide sequences
US20090111104A1 (en) * 2005-11-04 2009-04-30 Fluorotechnics Pty Limited Method for Monitoring Hydrolytic Activity
US20090068643A1 (en) * 2005-11-23 2009-03-12 Integrated Dna Technologies, Inc. Dual Function Primers for Amplifying DNA and Methods of Use
US10227641B2 (en) 2008-04-30 2019-03-12 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US9434988B2 (en) 2008-04-30 2016-09-06 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP2644709B1 (en) 2008-04-30 2014-12-17 Integrated Dna Technologies, Inc. RNase-H-based assays utilizing modified RNA monomers
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP2401388A4 (en) 2009-02-23 2012-12-05 Univ Georgetown Sequence-specific detection of nucleotide sequences
WO2011060014A1 (en) * 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
US8618253B2 (en) 2010-05-25 2013-12-31 Samsung Techwin Co., Ltd. Modified RNAse H and detection of nucleic acid amplification
KR20120046018A (en) * 2010-10-04 2012-05-09 삼성테크윈 주식회사 Real time pcr detection of single nucleotide polymorphisms
CN102321759B (en) * 2011-08-25 2013-05-08 南京邮电大学 Fluorescence method for detecting S1 nuclease and inhibitor thereof
WO2013081206A1 (en) * 2011-11-30 2013-06-06 삼성테크윈 주식회사 Real-time pcr detection using stabilized probe
JP2016536995A (en) 2013-11-14 2016-12-01 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド DNA polymerase mutants with increased template discrimination activity
WO2015195949A2 (en) 2014-06-18 2015-12-23 Clear Gene, Inc. Methods, compositions, and devices for rapid analysis of biological markers
CN108026575B (en) 2015-07-17 2022-08-19 哈佛学院董事及会员团体 Method for amplifying nucleic acid sequence
US11401558B2 (en) 2015-12-18 2022-08-02 Clear Gene, Inc. Methods, compositions, kits and devices for rapid analysis of biological markers
WO2017142297A1 (en) 2016-02-15 2017-08-24 주식회사 누리바이오 Single-stranded nucleic acid for detecting nucleic acid or protein in real time and detection method using same
KR101901749B1 (en) 2016-02-15 2018-09-28 주식회사 누리바이오 Promer for Real-Time Detection of Nucleic Acid or Protein and Method of Detecting Nucleic Acid or Protein Using the Same
CN109476695A (en) 2016-06-27 2019-03-15 丹娜法伯癌症研究院 Method for measuring RNA translation rate
CN110023507B (en) * 2016-09-27 2024-01-23 纽丽生物科技有限公司 Method for detecting small RNAs or proteins associated with small RNAs
WO2023278704A1 (en) 2021-06-30 2023-01-05 Dana-Farber Cancer Institute, Inc. Compositions and methods for enrichment of nucleic acids using light-mediated cross-linking

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683194A (en) * 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4876187A (en) * 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
US4996143A (en) * 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
EP0365627B1 (en) * 1988-03-24 1993-12-22 University Of Iowa Research Foundation Catalytic hybridization systems for the detection of nucleic acid sequences based on their activity as cofactors in catalytic reactions in which a complementary labeled nucleic acid probe is cleaved
WO1989009835A1 (en) * 1988-04-08 1989-10-19 The Salk Institute For Biological Studies Ligase-based amplification method
US5001769A (en) * 1988-12-20 1991-03-19 Educational Testing Service Image processing system
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase

Also Published As

Publication number Publication date
EP0803058A4 (en) 2001-08-16
CA2209080C (en) 2008-07-29
WO1996021144A1 (en) 1996-07-11
US5763181A (en) 1998-06-09
DE69535882D1 (en) 2008-12-18
EP0803058B1 (en) 2008-11-05
EP0803058A1 (en) 1997-10-29

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Effective date: 20151229