CA2211367A1 - Improved method for reverse transcription - Google Patents

Improved method for reverse transcription

Info

Publication number
CA2211367A1
CA2211367A1 CA002211367A CA2211367A CA2211367A1 CA 2211367 A1 CA2211367 A1 CA 2211367A1 CA 002211367 A CA002211367 A CA 002211367A CA 2211367 A CA2211367 A CA 2211367A CA 2211367 A1 CA2211367 A1 CA 2211367A1
Authority
CA
Canada
Prior art keywords
mrna
reverse transcription
reverse transcriptase
improved method
metal ions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002211367A
Other languages
French (fr)
Other versions
CA2211367C (en
Inventor
Yoshihide Hayashizaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dnaform KK
RIKEN Institute of Physical and Chemical Research
Original Assignee
Yoshihide Hayashizaki
The Institute Of Physical And Chemical Research
Kabushiki Kaisha Dnaform
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP19633196A external-priority patent/JP3709490B2/en
Application filed by Yoshihide Hayashizaki, The Institute Of Physical And Chemical Research, Kabushiki Kaisha Dnaform filed Critical Yoshihide Hayashizaki
Priority to CA2512701A priority Critical patent/CA2512701C/en
Publication of CA2211367A1 publication Critical patent/CA2211367A1/en
Application granted granted Critical
Publication of CA2211367C publication Critical patent/CA2211367C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for preparing a cDNA from a mRNA using a reverse transcriptase wherein reverse transcription is performed at a temperature at which the mRNA does not take a secondary structure, for example, at a temperature of 45°C or more. The method is performed, for example, using a heat-labile reverse transcriptase in the presence of a substance exhibiting chaperone function having chaperone function such as saccharides. The method is performed, for example, in the presence of metal ions necessary for activation of the reverse transcriptase and a chelating agent for the metal ions such as a deoxynucleotide triphosphate. The method is capable of reverse transcription over the full length of mRNA template even if the mRNA is a long chain mRNA and, as a result, producing a full length cDNA.
CA002211367A 1996-07-25 1997-07-24 Improved method for reverse transcription Expired - Fee Related CA2211367C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2512701A CA2512701C (en) 1996-07-25 1997-07-24 Improved method for reverse transcription

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP196331/1996 1996-07-25
JP19632996 1996-07-25
JP19633196A JP3709490B2 (en) 1996-07-25 1996-07-25 Method for improving the thermal stability of RNA
JP196329/1996 1996-07-25

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2512701A Division CA2512701C (en) 1996-07-25 1997-07-24 Improved method for reverse transcription

Publications (2)

Publication Number Publication Date
CA2211367A1 true CA2211367A1 (en) 1998-01-25
CA2211367C CA2211367C (en) 2008-04-22

Family

ID=26509679

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002211367A Expired - Fee Related CA2211367C (en) 1996-07-25 1997-07-24 Improved method for reverse transcription

Country Status (4)

Country Link
US (3) US6013488A (en)
EP (2) EP0821059B8 (en)
CA (1) CA2211367C (en)
DE (2) DE69738354D1 (en)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998047912A1 (en) 1997-04-22 1998-10-29 Life Technologies, Inc. Methods for the production of aslv reverse transcriptases composed of multiple subunits
WO1998059075A1 (en) 1997-06-25 1998-12-30 Life Technologies, Inc. Improved method for isolating and recovering target dna or rna molecules having a desired nucleotide sequence
US6787305B1 (en) * 1998-03-13 2004-09-07 Invitrogen Corporation Compositions and methods for enhanced synthesis of nucleic acid molecules
JP4854853B2 (en) 1998-11-12 2012-01-18 ライフ テクノロジーズ コーポレーション Transfection drug
US7074556B2 (en) * 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
EP1165760A2 (en) * 1999-03-12 2002-01-02 McGill University A method for increasing the processivity of a dna- or rna-dependent polymerase and compositions therefor
US6271004B1 (en) * 1999-06-25 2001-08-07 Display Systems Biotech A/S Method for improved reverse transcription at high temperatures
US6835537B1 (en) 2000-09-29 2004-12-28 Myriad Genetics, Inc. Method for equalizing band intensities on sequencing gels
DE10105208A1 (en) * 2001-02-06 2002-08-14 Ihf Inst Fuer Hormon Und Fortp Enzymatic production of cDNA by reverse transcription, in the presence of betaine, provides a high yield of full-length molecules from large RNA templates
ES2188357B1 (en) * 2001-03-12 2005-01-01 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. METHOD FOR AMPLIFICATION BY TRANSCRIPTION IN REVERSE COUPLED TO A REACTION OF NESTED OR SEMI-NESTED PCR IN ONE STEP, OF A DIANA RNA, AND ITS APPLICATIONS IN THE IDENTIFICATION OF ENTEROVIRUS AND IN THE DISCRIMINATION BETWEEN POLIOVIRUS AND NON-POLIO.
EP1523554A2 (en) * 2002-06-12 2005-04-20 Riken Method of utilizing the 5' end of transcribed nucleic acid regions for cloning and analysis
US20040152072A1 (en) * 2002-07-30 2004-08-05 Invitrogen Corporation Reverse transcription
US20060084799A1 (en) * 2003-09-24 2006-04-20 Williams Lewis T Human cDNA clones comprising polynucleotides encoding polypeptides and methods of their use
US20070274988A1 (en) * 2003-10-10 2007-11-29 Five Prime Therapeautics, Inc. Kiaa0779, Splice Variants Thereof, and Methods of Their Use
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
JP4896006B2 (en) 2004-04-08 2012-03-14 バイオマトリカ， インコーポレイテッド Integration with sample storage and sample management for life sciences
US20080176209A1 (en) * 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
JP5187847B2 (en) 2005-05-06 2013-04-24 ジェン−プローブ・インコーポレーテッド Nucleic acid target capture method
WO2007011901A2 (en) * 2005-07-15 2007-01-25 Applera Corporation Hot start reverse transcription by primer design
WO2012018638A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
US9845489B2 (en) 2010-07-26 2017-12-19 Biomatrica, Inc. Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
WO2012054727A1 (en) 2010-10-22 2012-04-26 Bio-Rad Laboratories, Inc. Reverse transcriptase mixtures with improved storage stability
FR2984357B1 (en) 2011-12-16 2016-11-18 Biomerieux Sa PROCESS FOR TRANSCRIPTIONAL AMPLIFICATION OF NUCLEIC ACIDS COVERING STEPS OF DIFFERENT TEMPERATURES
WO2014100755A2 (en) 2012-12-20 2014-06-26 Biomatrica, Inc. Formulations and methods for stabilizing pcr reagents
EP3097190A2 (en) 2014-01-22 2016-11-30 Life Technologies Corporation Novel reverse transcriptases for use in high temperature nucleic acid synthesis
ES2786373T3 (en) 2014-06-10 2020-10-09 Biomatrica Inc Platelet stabilization at room temperatures
WO2016011203A1 (en) 2014-07-15 2016-01-21 Life Technologies Corporation Compositions with lipid aggregates and methods for efficient delivery of molecules to cells
ES2946184T3 (en) 2015-12-08 2023-07-13 Biomatrica Inc Reduced erythrocyte sedimentation rate
EA037304B1 (en) 2016-01-27 2021-03-09 КАБУСИКИ КАЙСЯ ДиЭнЭйФОРМ Method for decoding base sequence of nucleic acid corresponding to end region of rna and method for analyzing dna element

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0035204B2 (en) * 1980-03-05 1991-05-22 Miles Inc. Pasteurized therapeutically active protein compositions
US4409200A (en) * 1980-04-28 1983-10-11 Research Corporation Reverse transcriptase from human milk, method for its purification, and its use in the detection of breast cancer
US5017492A (en) * 1986-02-27 1991-05-21 Life Technologies, Inc. Reverse transcriptase and method for its production
US5407800A (en) * 1986-08-22 1995-04-18 Hoffmann-La Roche Inc. Reverse transcription with Thermus thermophilus polymerase
US5310652A (en) * 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
AU656315B2 (en) * 1989-12-22 1995-02-02 F. Hoffmann-La Roche Ag pigh temperature reverse transcriptases
DE4124286A1 (en) * 1991-07-22 1993-01-28 Boehringer Mannheim Gmbh METHOD FOR STABILIZING PROTEINS IN OPTICAL TESTS
FR2690691B1 (en) 1992-04-29 1999-02-12 Bio Merieux METHOD OF AMPLIFYING RNA REQUIRING A SINGLE HANDLING STAGE.
WO1994003472A1 (en) * 1992-08-04 1994-02-17 Gen-Probe Incorporated Nucleic acid sequence amplification
WO1994012657A1 (en) * 1992-11-20 1994-06-09 Katcher Harold L Direct-phenol pcr, rt and rt-pcr methods
DE4239969A1 (en) * 1992-11-27 1994-06-01 Boehringer Mannheim Gmbh Process for the stabilization of proteins
AU6945694A (en) * 1993-05-06 1994-12-12 Dade International Inc. Human papillomavirus detection assay
WO1995014770A1 (en) * 1993-11-25 1995-06-01 Pacific Enzymes (1993) Limited Improved polymerase
AU694479B2 (en) * 1994-01-31 1998-07-23 Regents Of The University Of California, The Methods for the elimination of DNA sequencing artifacts
DE4411588C1 (en) * 1994-03-30 1995-09-28 Deutsches Rheuma Forschungszen Buffer, contg. betaine, for RNA- and DNA-polymerase reactions
US5556771A (en) * 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification

Also Published As

Publication number Publication date
EP0821059A3 (en) 1998-04-22
EP0821059B1 (en) 2005-12-21
US20010012617A1 (en) 2001-08-09
DE69738354D1 (en) 2008-01-17
US6013488A (en) 2000-01-11
EP1243661A1 (en) 2002-09-25
US6221599B1 (en) 2001-04-24
EP0821059B8 (en) 2006-03-08
EP1243661B1 (en) 2007-12-05
CA2211367C (en) 2008-04-22
EP0821059A2 (en) 1998-01-28
DE69734913D1 (en) 2006-01-26
US6372437B2 (en) 2002-04-16
DE69734913T2 (en) 2006-08-24

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EEER Examination request
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Effective date: 20140724