CA2212193A1 - Method of detecting biological materials using a polyvinylidene fluoride membrane - Google Patents

Method of detecting biological materials using a polyvinylidene fluoride membrane

Info

Publication number
CA2212193A1
CA2212193A1 CA002212193A CA2212193A CA2212193A1 CA 2212193 A1 CA2212193 A1 CA 2212193A1 CA 002212193 A CA002212193 A CA 002212193A CA 2212193 A CA2212193 A CA 2212193A CA 2212193 A1 CA2212193 A1 CA 2212193A1
Authority
CA
Canada
Prior art keywords
membrane
polyvinylidene fluoride
biological materials
fluoride membrane
detecting biological
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002212193A
Other languages
French (fr)
Other versions
CA2212193C (en
Inventor
Richard Frank Salinaro
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pall Corp
Original Assignee
Pall Corporation
Richard Frank Salinaro
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pall Corporation, Richard Frank Salinaro filed Critical Pall Corporation
Publication of CA2212193A1 publication Critical patent/CA2212193A1/en
Application granted granted Critical
Publication of CA2212193C publication Critical patent/CA2212193C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/5436Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand physically entrapped within the solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/25Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

The present invention provides an improved method of detecting or transferring a biological material whereby a biological material is adhered to a membrane which is then contacted with a detecting reagent, wherein the improvement comprises utilizing a polyvinylidene fluoride membrane which has been subjected to a temperature of at least about 80°C
but less than the temperature at which the membrane softens and deforms for a time sufficient to reduce the ability of the detecting reagent to directly adhere to the membrane.
CA002212193A 1995-04-11 1996-03-20 Method of detecting biological materials using a polyvinylidene fluoride membrane Expired - Lifetime CA2212193C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/420,218 US5567626A (en) 1995-04-11 1995-04-11 Method of detecting biological materials using a polyvinyllidene fluoride membrane
US08/420,218 1995-04-11
PCT/US1996/004139 WO1996032643A1 (en) 1995-04-11 1996-03-20 Method of detecting biological materials using a polyvinylidene fluoride membrane

Publications (2)

Publication Number Publication Date
CA2212193A1 true CA2212193A1 (en) 1996-10-17
CA2212193C CA2212193C (en) 2001-10-09

Family

ID=23665554

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002212193A Expired - Lifetime CA2212193C (en) 1995-04-11 1996-03-20 Method of detecting biological materials using a polyvinylidene fluoride membrane

Country Status (7)

Country Link
US (1) US5567626A (en)
EP (1) EP0820592B1 (en)
JP (1) JP2996514B2 (en)
CA (1) CA2212193C (en)
DE (1) DE69603716T2 (en)
GB (1) GB2313836B (en)
WO (1) WO1996032643A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003239332A1 (en) * 2002-05-03 2003-11-17 Pall Corporation Blended polymer membrane media for treating aqueous fluids
US20080073622A1 (en) * 2006-09-22 2008-03-27 Inolex Investment Corporation Conductive polyurethane foam and methods for making same
JP2009249439A (en) * 2008-04-02 2009-10-29 Lintec Corp Modified polyvinylidene fluoride membrane, laminated membrane for protein adsorption, and manufacturing method thereof
JP2011242159A (en) * 2010-05-14 2011-12-01 Yokohama City Univ Method for transferring protein separated by electrophoresis onto membrane filter and performing mass spectrometry

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4302204A (en) * 1979-07-02 1981-11-24 The Board Of Trustees Of Leland Stanford Junior University Transfer and detection of nucleic acids
US4341605A (en) * 1981-01-16 1982-07-27 E. I. Du Pont De Nemours And Company Process for cation permeable membrane with reinforcement fabric embedded therein and product thereof
US4455370A (en) * 1982-05-17 1984-06-19 E. I. Du Pont De Nemours And Company Transferring separated components in gel electrophoresis via nylon membrane
US4618533A (en) * 1984-11-30 1986-10-21 Millipore Corporation Porous membrane having hydrophilic surface and process
US4923620A (en) * 1987-10-20 1990-05-08 Pall Corporation Device for depletion of the leukocyte content of blood and blood components
US5004543A (en) * 1988-06-21 1991-04-02 Millipore Corporation Charge-modified hydrophobic membrane materials and method for making the same
US5200312A (en) * 1989-01-30 1993-04-06 The United States Of America As Represented By The Secretary Of The Navy Membrane based dot immunoassay and method of use
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5071909A (en) * 1989-07-26 1991-12-10 Millipore Corporation Immobilization of proteins and peptides on insoluble supports
US5079272A (en) * 1989-11-30 1992-01-07 Millipore Corporation Porous membrane formed from interpenetrating polymer network having hydrophilic surface
US5198505A (en) * 1991-04-11 1993-03-30 Pall Corporation Uniform polyvinylidene difluoride membranes
US5196508A (en) * 1991-04-11 1993-03-23 Pall Corporation Method for making uniform polyvinylidene difluoride membranes
CA2071736A1 (en) * 1991-06-24 1992-12-25 Naoyuki Kouno Acid dye staining method
US5240615A (en) * 1991-08-20 1993-08-31 Fishman Jerry H Composite membrane composed of microporous polyvinylidene difluoride membrane laminated to porous support and process for its preparation
US5183607A (en) * 1991-12-17 1993-02-02 Beall George H Polymer membranes for separation process
US5283186A (en) * 1991-12-31 1994-02-01 Abbott Laboratories Preparation of a compressed membrane containing immobilized biologically acting material

Also Published As

Publication number Publication date
EP0820592B1 (en) 1999-08-11
GB9716671D0 (en) 1997-10-15
US5567626A (en) 1996-10-22
EP0820592A1 (en) 1998-01-28
JPH10508106A (en) 1998-08-04
WO1996032643A1 (en) 1996-10-17
GB2313836A (en) 1997-12-10
JP2996514B2 (en) 2000-01-11
DE69603716D1 (en) 1999-09-16
DE69603716T2 (en) 2000-01-13
CA2212193C (en) 2001-10-09
GB2313836B (en) 1999-05-05

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Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20160321