CA2212193A1 - Method of detecting biological materials using a polyvinylidene fluoride membrane - Google Patents
Method of detecting biological materials using a polyvinylidene fluoride membraneInfo
- Publication number
- CA2212193A1 CA2212193A1 CA002212193A CA2212193A CA2212193A1 CA 2212193 A1 CA2212193 A1 CA 2212193A1 CA 002212193 A CA002212193 A CA 002212193A CA 2212193 A CA2212193 A CA 2212193A CA 2212193 A1 CA2212193 A1 CA 2212193A1
- Authority
- CA
- Canada
- Prior art keywords
- membrane
- polyvinylidene fluoride
- biological materials
- fluoride membrane
- detecting biological
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/5436—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand physically entrapped within the solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
- Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction
Abstract
The present invention provides an improved method of detecting or transferring a biological material whereby a biological material is adhered to a membrane which is then contacted with a detecting reagent, wherein the improvement comprises utilizing a polyvinylidene fluoride membrane which has been subjected to a temperature of at least about 80°C
but less than the temperature at which the membrane softens and deforms for a time sufficient to reduce the ability of the detecting reagent to directly adhere to the membrane.
but less than the temperature at which the membrane softens and deforms for a time sufficient to reduce the ability of the detecting reagent to directly adhere to the membrane.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/420,218 US5567626A (en) | 1995-04-11 | 1995-04-11 | Method of detecting biological materials using a polyvinyllidene fluoride membrane |
US08/420,218 | 1995-04-11 | ||
PCT/US1996/004139 WO1996032643A1 (en) | 1995-04-11 | 1996-03-20 | Method of detecting biological materials using a polyvinylidene fluoride membrane |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2212193A1 true CA2212193A1 (en) | 1996-10-17 |
CA2212193C CA2212193C (en) | 2001-10-09 |
Family
ID=23665554
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002212193A Expired - Lifetime CA2212193C (en) | 1995-04-11 | 1996-03-20 | Method of detecting biological materials using a polyvinylidene fluoride membrane |
Country Status (7)
Country | Link |
---|---|
US (1) | US5567626A (en) |
EP (1) | EP0820592B1 (en) |
JP (1) | JP2996514B2 (en) |
CA (1) | CA2212193C (en) |
DE (1) | DE69603716T2 (en) |
GB (1) | GB2313836B (en) |
WO (1) | WO1996032643A1 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2003239332A1 (en) * | 2002-05-03 | 2003-11-17 | Pall Corporation | Blended polymer membrane media for treating aqueous fluids |
US20080073622A1 (en) * | 2006-09-22 | 2008-03-27 | Inolex Investment Corporation | Conductive polyurethane foam and methods for making same |
JP2009249439A (en) * | 2008-04-02 | 2009-10-29 | Lintec Corp | Modified polyvinylidene fluoride membrane, laminated membrane for protein adsorption, and manufacturing method thereof |
JP2011242159A (en) * | 2010-05-14 | 2011-12-01 | Yokohama City Univ | Method for transferring protein separated by electrophoresis onto membrane filter and performing mass spectrometry |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4302204A (en) * | 1979-07-02 | 1981-11-24 | The Board Of Trustees Of Leland Stanford Junior University | Transfer and detection of nucleic acids |
US4341605A (en) * | 1981-01-16 | 1982-07-27 | E. I. Du Pont De Nemours And Company | Process for cation permeable membrane with reinforcement fabric embedded therein and product thereof |
US4455370A (en) * | 1982-05-17 | 1984-06-19 | E. I. Du Pont De Nemours And Company | Transferring separated components in gel electrophoresis via nylon membrane |
US4618533A (en) * | 1984-11-30 | 1986-10-21 | Millipore Corporation | Porous membrane having hydrophilic surface and process |
US4923620A (en) * | 1987-10-20 | 1990-05-08 | Pall Corporation | Device for depletion of the leukocyte content of blood and blood components |
US5004543A (en) * | 1988-06-21 | 1991-04-02 | Millipore Corporation | Charge-modified hydrophobic membrane materials and method for making the same |
US5200312A (en) * | 1989-01-30 | 1993-04-06 | The United States Of America As Represented By The Secretary Of The Navy | Membrane based dot immunoassay and method of use |
US5234809A (en) * | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
US5071909A (en) * | 1989-07-26 | 1991-12-10 | Millipore Corporation | Immobilization of proteins and peptides on insoluble supports |
US5079272A (en) * | 1989-11-30 | 1992-01-07 | Millipore Corporation | Porous membrane formed from interpenetrating polymer network having hydrophilic surface |
US5198505A (en) * | 1991-04-11 | 1993-03-30 | Pall Corporation | Uniform polyvinylidene difluoride membranes |
US5196508A (en) * | 1991-04-11 | 1993-03-23 | Pall Corporation | Method for making uniform polyvinylidene difluoride membranes |
CA2071736A1 (en) * | 1991-06-24 | 1992-12-25 | Naoyuki Kouno | Acid dye staining method |
US5240615A (en) * | 1991-08-20 | 1993-08-31 | Fishman Jerry H | Composite membrane composed of microporous polyvinylidene difluoride membrane laminated to porous support and process for its preparation |
US5183607A (en) * | 1991-12-17 | 1993-02-02 | Beall George H | Polymer membranes for separation process |
US5283186A (en) * | 1991-12-31 | 1994-02-01 | Abbott Laboratories | Preparation of a compressed membrane containing immobilized biologically acting material |
-
1995
- 1995-04-11 US US08/420,218 patent/US5567626A/en not_active Expired - Lifetime
-
1996
- 1996-03-20 EP EP96909874A patent/EP0820592B1/en not_active Expired - Lifetime
- 1996-03-20 WO PCT/US1996/004139 patent/WO1996032643A1/en active IP Right Grant
- 1996-03-20 DE DE69603716T patent/DE69603716T2/en not_active Expired - Lifetime
- 1996-03-20 CA CA002212193A patent/CA2212193C/en not_active Expired - Lifetime
- 1996-03-20 GB GB9716671A patent/GB2313836B/en not_active Expired - Lifetime
- 1996-03-20 JP JP8531024A patent/JP2996514B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
EP0820592B1 (en) | 1999-08-11 |
GB9716671D0 (en) | 1997-10-15 |
US5567626A (en) | 1996-10-22 |
EP0820592A1 (en) | 1998-01-28 |
JPH10508106A (en) | 1998-08-04 |
WO1996032643A1 (en) | 1996-10-17 |
GB2313836A (en) | 1997-12-10 |
JP2996514B2 (en) | 2000-01-11 |
DE69603716D1 (en) | 1999-09-16 |
DE69603716T2 (en) | 2000-01-13 |
CA2212193C (en) | 2001-10-09 |
GB2313836B (en) | 1999-05-05 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKEX | Expiry |
Effective date: 20160321 |