CA2218017A1 - Dna sequencing by parallel oligonucleotide extensions - Google Patents

Dna sequencing by parallel oligonucleotide extensions

Info

Publication number
CA2218017A1
CA2218017A1 CA002218017A CA2218017A CA2218017A1 CA 2218017 A1 CA2218017 A1 CA 2218017A1 CA 002218017 A CA002218017 A CA 002218017A CA 2218017 A CA2218017 A CA 2218017A CA 2218017 A1 CA2218017 A1 CA 2218017A1
Authority
CA
Canada
Prior art keywords
extended
duplex
template
dna fragments
obviates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002218017A
Other languages
French (fr)
Other versions
CA2218017C (en
Inventor
Stephen C. Macevicz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lynx Therapeutics Inc
Original Assignee
Lynx Therapeutics, Inc.
Stephen C. Macevicz
Spectragen, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23683421&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2218017(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Lynx Therapeutics, Inc., Stephen C. Macevicz, Spectragen, Inc. filed Critical Lynx Therapeutics, Inc.
Publication of CA2218017A1 publication Critical patent/CA2218017A1/en
Application granted granted Critical
Publication of CA2218017C publication Critical patent/CA2218017C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Abstract

Method and compositions are provided for analyzing nucleic acid sequences based on repeated cycles of duplex extension along a single-stranded template.
Preferably, such extension starts from a duplex formed between an initializing oligonucleotide and the template. As illustrated in the figure, the initializing oligonucleotide is extended in an initial extension cycle by ligating an oligonucleotide probe to its end to form an extended duplex. The extended duplex is then repeatedly extended by subsequent cycles of ligation.
During each cycle, the identity of one or more nucleotides in the template is determined by a label on, or associated with, a successfully ligated oligonucleotide probe. The invention provides a method of sequencing nucleic acid which obviates electrophoretic separation of similarly sized DNA
fragments, and which eliminates the difficulties associated with the detection and analysis of spacially overlapping bands of DNA fragments in a gel or like medium. The invention also obviates the need to generate DNA fragments from long single-stranded templates with a DNA polymerase.
CA002218017A 1995-04-17 1996-04-16 Dna sequencing by parallel oligonucleotide extensions Expired - Lifetime CA2218017C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/424,663 1995-04-17
US08/424,663 US5750341A (en) 1995-04-17 1995-04-17 DNA sequencing by parallel oligonucleotide extensions
PCT/US1996/005245 WO1996033205A1 (en) 1995-04-17 1996-04-16 Dna sequencing by parallel oligonucleotide extensions

Publications (2)

Publication Number Publication Date
CA2218017A1 true CA2218017A1 (en) 1996-10-24
CA2218017C CA2218017C (en) 2003-12-02

Family

ID=23683421

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002218017A Expired - Lifetime CA2218017C (en) 1995-04-17 1996-04-16 Dna sequencing by parallel oligonucleotide extensions

Country Status (8)

Country Link
US (3) US5750341A (en)
EP (5) EP2433950A1 (en)
JP (5) JP4546582B2 (en)
AU (1) AU718937B2 (en)
CA (1) CA2218017C (en)
DE (1) DE10184524T1 (en)
DK (1) DK2298787T3 (en)
WO (1) WO1996033205A1 (en)

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