CA2223505C - Anti-fungal and anti-bacterial histatin-based peptides - Google Patents

Abstract

Histatin-based peptides representing defined portions of the amino acid sequences of naturally occurring human histatins and methods for treatment o f fungal or bacterial infection are described. These histatin-based peptides represent the active anti-fungal and anti-bacterial region of naturally occurring human histatins.

Description

ANTI-FUNGAL AND ANTI-BACTERIAL HISTATIN-BASED PEPTIDES
GOVERNMENT SUPPORT
The invention described herein was supported in whole or in part by Grant No. DE07652 from the National Institutes of Health, which have certain rights in the invention.
BACKGROUND OF THE :INVENTION
The family of naturally occurring human histatins is a group of twelve low molecular weight, abundant in 20 histidine, peptide: found in human submandibular and parotid salivary sEecretions (Oppenheim et al. (1986), J. Biol. Chem. 261: 1177-1182; Oppenheim et al. (1988), J. Biol. Chem. 263: 7472-7477; Troxler et al. (1990), J. Dent. Res. 69: :?-6). The primary structure of the major 25 family members (hi:~tatins 1, 3, and 5; 70-80% of the whole ' family) has shown t=hat these proteins consist of 38, 32 and 24 amino acid residues, respectively. There is a high ' degree of homology among these three major histatins.
Histatin 5 results from post-translational cleavage of 30 histatin 3. Many of the smaller members of the histatin family may also, in fact, originate by post-translational proteolysis of histatins 1, 3 and 5 (Oppenheim et al.
(1989), Human Saliva: Clinical Chemistry and Microbiology Vol. 1 CRC Press, Boca Raton, FL, ed. Tenovuo, J.O.; Lal ~t al. (1992), Arch. Oral Biol. 37: 7-13). The genes that encode histatins 1 and 3 have been localized chromosomally (vanderSpek et al., (1989), Am. J. Hum. Genet. 45: 381-387) and sequenced (Sabatini, L.M. et al. (1989), Biochem.
$iophys. Res. Comm. 160:495-502). Histatins 1 and 3 appear to be derived from separate genes.
The three major human histatins exhibit specific antimicrobial activities towards diverse oral microbiota.
These histatins, at physiological concentrations, are capable of killing Candida albicans in both blastopore and mycelial forms (Pollock, J.J. et a1. (1984), Infect. Immun.
44:702-707; Xu, T. et a1. (1991), Infect. Immun. 59 (8):
2549-2554). Histatins are also capable of killing oral bacteria, including Streptococcus mutans (MacKay, B.J. et al. (1984), Infect. Immun. 44:695-701; Xu, T. et a1.
(1990), J. Dent. Res. 69: 239), Porphyromonas ainaivalis (Colon et al. (1993), J. Dent. Res. 72: 322) and Actinomyces viscosus (Kalpidis et al. (1992) J. Dent. Res.
72: 305).
Infection with the yeast Candida albicans is a prevalent and, in some cases, life-threatening condition affecting otherwise healthy and immuno-compromised patients. Candidal vaginitis is estimated to affect 15 to 55% of healthy young women. Candidal infections often occur in diabetics, during pregnancy, and following medication with antibiotics, steroid hormones, or oral contraceptives. (Tapper-Jones, L.M. et al. (1981) J. Clin.
Pathol. 34:706-11; Sobel, J.D. et al. (1984) Infect. Immun.
44:576-580). Oral candidiasis is an early opportunistic infection of Acquired Immune Deficiency Syndrome (AIDS) in individuals infected with human immunodeficiency virus type 1, as well as a complication of radiation and chemotherapy in cancer patients. (Yeh, C.-K. et a1. (1988) J. of Acquired Immune Deficiency Syndromes 1:361-366). In addition, candidal infection of denture wearers plays a primary role in dental stomatitis, a prevalent oral problem among the elderly. (Pollock, J.J. et a1. (1990) NYS
Dental J. 56:36-38). Candidal infections of skin and urethra are widespread problems. In patients in intensive care and immuno-compromised patients, systemic fungal infection often leads to death, since there are few safe and effective anti-fungal pharmaceuticals for intravenous use. (Burnie, J.P. et a1. (1985) British Medical Journal 290:746-748). Similarly, infections with various bacterial species can cause severe disease states and even death.
Although several anti-fungal agents (e. g., clotrimazole, miconazole, ketoconazole, and nystatin) and anti-bacterial agents (penicillin, streptomycin, tetracycline and chlorhexidine) are currently available, these agents are not completely effective, can lead to drug resistant organisms and can produce adverse side effects.
Many are not appropriate for oral or systemic administration. Thus, a potent, naturally occurring anti-fungal or anti-bacterial substance would provide a significant improvement in the treatment of microbial infection.
SUMMARY OF THE INVENTION
This invention is based on substantially pure peptides which have anti-candidal or anti-bacterial activity which are equivalent to that of naturally occurring histatins but are smaller in size. These peptides represent defined portions of the amino acid sequences of naturally occurring human histidine-rich salivary proteins called histatins, which will be referred to herein as histatin-based peptides. The histatin-based peptides of this invention WO 96/40768 PCTlUS96/09374 also include defined portions of the amino acid sequences of histatins with specific amino acid substitutions at specified positions of the sequences. As demonstrated herein, these histatin-based peptides have been shown to be superior, particularly on a weight basis, in anti-candidal or anti-bacterial activity over the naturally occurring histatins. Thus, this invention provides compositions for treatment of fungal or bacterial infection comprising histatin-based peptides with defined amino acid sequences.
l0 These peptides are derived from a specific histatin-based peptide having a specified 12 amino acid sequence. This peptide is designated as peptide 113 (SEQ ID NO: 18).
Other peptides with significant anti-fungal or anti-bacterial activities have sequence portions of at least 8 amino acids from this histatin-based peptide or are homologs of peptide 113 with amino acid substitutions at particular positions in the peptide.
BRIEF DESCRIPTION OF FIGURES
Figure lA-1D shows the amino acid sequences of human histatins and peptides 101, 102, 103, 104, 105, 113, 113-F4, 113-F5, 113-F12, 113-F4.5, 113-F4.5.12, 113-K6, 113-H8, 113-K6H8, 113-F8, 113-L4.5.12, 113-Y4.5.12, 113-Q2.10, 113-Q3.9, 113-Q2.3.9.10, 117, 118, 119, 120 and 129.
Figure 2 is a graph that shows the o killing of C.
albicans blastoconidia as a function of the concentration of histatin-5, peptide 103, peptide 113 and peptide 129.
Figure 3 is a graph that shows the ~ killing of C.
Albicans blastoconidia as a function of concentration of peptide 113, peptide 113-F4, peptide 113-F5, peptide 113-F12, peptide 113-F4.5 and peptide 113-F4.5.12.
Figure 4 is a graph that shows the % killing of C.
albicans blastoconidia as a function of concentration of peptide 113, peptide 113-K6, peptide 113-H8, peptide 113-K6H8, peptide 113-F8, peptide 113 with an acetyl group on the N-terminus (NA) and peptide 113 with a ca~__~bamyl group on the N-terminus (NC).
Figure 5 is a graph that shows the % killing of C. albicans blastoconidia as a function of concentration of peptide 113, peptide 113-L4.5.12, peptide 113-Y4"5.12, peptide 113-Q2.10, peptide 113-Q3.9 and peptide 113-Q2.3.9.10.
Figure 6 is a graph that shows the % killing of C. albicans blastoconidia as a function of concentration of peptide 113 and peptide 113-F4.5.12 at pH 7.4 and at pH
4Ø
Figure 7 is a graph that shows the amount of.- growth inhibition of P. gingivalis as a function of time for histatin 5, peptide 103, peptide 113 and peptide 129, as well as when no histatin-based peptide is present.
Figure 8 is a graph that shows the % killing of P. aeruginosa as a function of concentration of peptide 113, peptide 113-K6, peptide 113-H8, peptide 113-K6H8, peptide 113-F4.5.12 and peptide 113 with an acetyl group on the N-terminus (NA).
Figure 9 is a graph that shows the % killing of P. aeruginosa as a function of concentration of peptide 113, peptide 113-Y4.5.12, peptide 113-L4.5.I2, peptide 113-Q2.10, peptide 113-Q3.9 and peptide 113-Q2.3.9.10.
Figure 10 is a graph that shows the % inhibition of clostripain activity as a function of the concentration of histatin 5, peptide 101, peptide 103, peptide 105, peptide 118, peptide 119, peptide 120 and peptide 129.
Figure 11 is a graph that shows the % inhibition of clostripain activi-ty as a function of the concentration of peptide 113 and peptide 113-F4.5.12.

DETAILED DESCRIPTION OF THE INVENTION
This invention relates to peptides which have anti-fungal or anti-bacterial activity, in which the amino , acid sequences represent defined portions of the amino acid sequences of naturally occurring human histidine-rich salivary proteins called histatins. (Histatins are also referred to in the literature as histidine-rich proteins or HRPs.) Histatins are major salivary proteins which are synthesized in the parotid and submandibular-sublingual secretory glands of humans and Old World monkeys. (Azen, E.A. (1978) Biochem. Genet. 16:79-99). Histatins are believed to be part of an extraimmunologic defense system of the oral cavity. The anti-fungal activity of histatins, as well as their inhibitory effect on several oral bacteria (such as the cariogenic Streptococcus mutans and the periodontal pathogen Porphyromonas gingivalis), have been demonstrated in vitro. In addition, the observation that polyhistidine peptides inactivate herpes simplex virus in vitro and that whole saliva contains inhibitors of human immunodeficiency virus suggests the possibility that histatins may have anti-viral activity. These in vitro studies support potential clinical use of compositions containing histatins or histatin-based peptides for the treatment of local and systemic candidal infection, oral bacterial diseases, such as caries and periodontitis, systemic bacterial infection and viral infection.
Vaginal, urethral, mucosal, respiratory, skin, ear, oral or ophthalmic fungal or bacterial infections are particularly susceptible to histatin-based peptide therapy. Microbes which are specifically amenable to histatin-based peptide therapy are: ' a) Candida albicans;
b) Actinomyces actinomycetemcomitans;
c) Actinomyces viscosus;
d) Bacteroides forsythus;

WO 96/40768 PCT/US~96/09374 e) Bacteriodes fragilis;

f) Bacteriodes gracilis;

g) Bacteriodes ureolyticus;

h) Campylobacter concisus;

i) Campylobacter rectus;

j) Campylobacter showae;

k) Campylobacter sputorum;

1) Capnocytophaga gingi valis;

m) Capnocytophaga ochracea;

l0 n) Capnocytophaga sputigena;

o) Clostridium histolyticum;

p) Eikenella corrodens;

q) Eubacterium nodatum;

r) Fusobacterium nucleatum;

s) Fusobacterium periodonticum;

t) Pep tostrep tococcus micros;

u) Porphyromonas endodontalis;

v) Porphyromonas gingivalis;

w) Prevotella intermedia;

x) Prevotella nigrescens;

y) Propionibacterium acnes;

z) Pseudomonas aeruginosa;

aa) Selenomonas noxia;

bb) Staphylocbccus aureus;

cc) Streptococcus constellatus;

dd) Streptococcus gordonii;

ee) Streptococcus intermedius;

ff) Streptococcus mutans;

gg) Streptococcus oralis;

hh) St-reptococcus pneumonia;

11) Streptococcus sanguis;

kk) Treponema denticola;

11) Treponema pectinovorum;

mm) Treponema socranskii;

nn) Veillonella parvula; and _g_ oo) Wolinella succinogenes.
The human histatin proteins have been isolated and sequenced. They have been shown to be a family of twelve related low molecular weight proteins. Comparison of the amino acid sequences of the histatins suggests that histatin 2 and histatins 4-12 may have originated from specific proteolytic cleavage of histatin 1 and histatin 3, respectively. (Oppenheim, F.G. et al. (1988), J. Biol.
Chem. 263:7472-77; Troxler, R.F. et a1. (1990), J. Dent.
l0 Res. 69(1):2-6). Cloning and sequence analysis of histatin cDNAs further suggest that the histatins are encoded by two homologous genetic loci, whose primary products are histatins 1 and 3. (Sabatini, L.M. et a1.
(1989), Biochem. Biophys. Res. Comm. 160:495-502;
Vanderspek, J.C. et a1. (1990), Arch. Oral Biol.
35 (2) :137-43) .
The amino acid sequences of the anti-fungal and anti-bacterial peptides of this invention represent all or defined portions of the amino acid sequence of peptide 113 (SEQ ID NO: 18). In addition, the anti-fungal and anti-bacterial peptides of this invention include all or defined portions of peptide 113 (SEQ ID NO: 18) with amino acid substitutions at particular positions of the peptide.
Preferred embodiments of this invention are peptide 113 itself (SEQ ID NO: 18); fragments of peptide 113 containing at least an 8 amino acid sequence from this peptide; an amino acid sequence of at least 8 amino acids from peptide 113 where the glycine at position 6 is replaced by lysine, arginine or another basic amino acid;
an amino acid sequence of at least 8 amino acids from peptide 113 where the lysine at position 8 is replaced by histidine, phenylalanine or another hydrophobic amino acid;
an amino acid sequence of at least 8 amino acids from peptide 113 where one or more of the histidines at WO 96/40768 PCT/US9~6/09374 _g_ . positions 4, 5 and 12 is (are) replaced by pheny7.alanine, tyrosine, leucine or another hydrophobic amino acid; an amino acid sequence of at least 8 amino acids from peptide 113 where one or both of the lysines at positions 2 and 10 is (are) replaced by glutamine, arginine or a combination of glutamine and arginine (when both lysines are replaced);
and an amino acid sequence of at least 8 amino acids from peptide 113 where one or both of the arginines at positions 3 and 9 is (are) replaced by glutamine, lysine or a combination of glutamine and lysine (when both arginines are replaced). Combinations of these amino acid replacements in an amino acid sequence of at least 8 amino acids from peptide 113 are all preferred embodiments of the invention provided that a combination of 4 glutamines or any other group of 4 non-basic amino acids at positions 2, 3, 9 and l0 does not occur.
Specific preferred embodiments of this invention are peptide 113 itself (SEQ ID NO: 18), histatin 11 (SEQ ID NO:
11), peptide 129 (SEQ ID NO: 23), peptide 117 (SEQ ID NO:
19), peptide 118 (SEQ ID NO: 20), peptide 119 (SEQ ID NO:
21), peptide 120 (SEQ ID NO: 22), peptide 113-F4 (SEQ ID
NO: 24), peptide 113-F5 (SEQ ID NO: 25), peptide 113-F12 (SEQ ID NO: 26), peptide 113-F4.5 (SEQ ID NO: 27), peptide 113-F4.5.12 (SEQ ID NO: 28), peptide 113-K6 (SEQ ID NO:
29), peptide 113-H8 (SEQ ID NO: 30), peptide 113-K6H8 (SEQ
ID NO: 31), peptide 113-F8 (SEQ ID NO: 32), peptide 113-L4.5.12 (SEQ ID NO: 33), peptide 113-Y4.5.12 (SEQ ID
NO: 34), peptide 113-Q2.10 (SEQ ID NO: 35), and peptide 113-Q3.9 (SEQ ID NO: 36). The amino acid sequences of these preferred peptides are shown in Figure lA-1D.
Combinations of two or more of these peptides are also effective as anti-fungal or anti-bacterial compositions and are included as compositions of the invention. However, the combination of these peptides where glutamine occurs at positions 2, 3, 9 and 10, i.e. peptide 113-Q2.3.9.10 (SEQ
ID NO: 37) is not a specifically preferred embodiment.
The peptides can be obtained from a naturally .
occurring source of histatin or they can be chemically synthesized or obtained by recombinant DNA techniques as expression products from cellular sources. These peptides can be altered by minor chemical modifications, such as by adding small substituents or by modifying one or more of the covalent bonds within or between the amino acid l0 residues, without significantly diminishing the anti-fungal or anti-bacterial activities of the peptides. Quite useful modifications are the addition of a substituent to either the amino terminus, the carboxyl terminus or to both ends of the peptide. These substituent addition modifications appear to stabilize the peptide in its active form and to aid in the prevention of enzymatic degradation of these peptides. These substituent groups are added to the amine, at the amino terminus, or to the carboxyl group, at the carboxyl terminus. The substituent groups can be somewhat bulky and may include one or more natural or modified amino acids. Particularly useful modifications are acetylation or carbamylation of the amino terminus~of the peptide or amidation of the carboxyl terminus of the peptide. A
combination of both modifications is especially useful.
Such modifications appear to increase the biological half-life of the peptides before degradation, encapsulation, internalization or excretion occurs.
The peptides described herein were tested in assays designed to measure separately their effectiveness in killing of blastoconidia of C. albicans in inhibiting the growth of P. gingivalis, in inhibiting hemagglutination caused by B. forsythus, and in inhibiting clostripain activity. These assays are indicative of anti-fungal and anti-bacterial activities of the histatin-based peptides of the present invention. When tested in these assays, the histatin-based peptides of this invention were found surprisingly to have superior anti-candidal and anti-. bacterial activity, particularly on an equivalent weight basis, when compared with histatin 5 as well as with histatin-based peptides 101-105. These anti-fungal and anti-bacterial activities are surprising in view of their size and truncated peptide form.
The following is a description of the histatin-based peptides, the antifungal activities of the histatin-based peptides as measured in assays for killing of Carrdida blastoconidia, and the anti-bacterial activities of the histatin-based peptides as measured in assays for inhibition of P. gingivalis growth, inhibition of hemagglutination caused by B. forsythus and inhibition of clostripain enzyme activity.
Histatin 5 and Histatin-Based Peptides Histatin 5 and the histatin-based peptides 101, 102, 103, 104, 105, 111, 113, 113-F4, 113-F5, 113-F12, 113-F4.5, 113-F4.5.12, 113-K6, 113-H8, 113-K6H8, 113-F8, 113-L4.5.12, 113-Y4.5.12, 113-Q2.10, 113-Q3.9, 113-Q2.3.9.10, 117, 118, 119, 120 and 129 were chemically synthesized. The amino acid sequences of histatin 5 and the synthesized histatin-based peptides are shown in Figure lA-1D.
Anti-Functal Activities of Histatin-Based Peptides C. albicarzs is a dimorphic yeast. It can exist in a yeast or blastoconidial form, which upon germination develops into the hyphal or germinated form. While the germinated form is considered to be more invasive, most of the C. albicans isolates harvested from the oral cavities of healthy individuals appear to be in the blastoconidial form. (Arendorf, T.M. et al. (1980), Arch. Oral Biol.
2~:1-10; Gow, N.A.R. et a1. (1987), Criti. Rev. Microbiol.
15:73-78; Odds, F.C. (1988), Candida and Candidosis, 2nd ed., Bailliere Tindall, London, England). Anti-fungal activity of synthetic histatin 5, histatin-based peptide 113, peptides bass=d on portions of the amino acid sequences of histatin-based peptide 113 and peptides derived from histatin-based peptide 113 with specified amino acid substitutions was measured in assays designed to test the effectiveness of t_he peptides against the blastoconidia ' form of Candida ('.Cable 1). These assays, which measure killing of blastoconidia of C. albicans, are described in Xu et al.
(Xu, T. et al. (1991), Infect. Immun. 59(8):2549-2554).
Peptide 113 was found to be about equipotent with h:istatin 5, demonstrating its anti-fungal activity despite its size in comparison with histatin 5. Peptides 101-105 demonstrated fungicidal activity comparable to that of histatin 5 and histatin-based peptide 113. Histatin 11, peptides 117-120 and peptide 129 all have demonstrable fungicidal activity even though they are smaller than peptide 113. There latter peptides appear to have the amino acid sequences of peptide 113 (and histatin 5) which are required for anti-fungal activity. The anti-fungal potency of the histatin-based peptide appears to be a function of both t_he size and the amino acid sequence of the respective peptide. In particular, the anti-fungal potency of human histatins appears to reside in peptide 113 with selected subpeptides of peptide 113 maintaining at least partial anti-fungal activity.
Modifications of peptide 113 by making particular types of amino acid substitutions in this peptide result in peptides that retain anti-fungal activity and in many instances display enhanced anti-fungal activity in comparison to peptide 113. For example, replacements of histidine with phE=nylalanine at positions 4, 5 or 12, either singly or :in combination, result in peptides with increased anti-fungal activities in comparison to peptide WO 96/40768 PC'T/US96/09374 113. Likewise, replacements of the glycine with lysine at position 6 or the lysine with histidine or phenylalanine at position 8, either' singly or in combination, result in other peptides with noticeably increased fungicidal activities in comparison to peptide 113. Acetylation or carbamylation of the N-terminus of peptide 113 also yields modified peptide 113 peptides with significant anti-fungal activity.
An additional feature of the directed amino acid substitutions of peptide 113 is that particular types of amino acid substitutions result in peptides with enhanced activities, e.g. anti-fungal, at a pH other than neutral.
For example, the substitution of histidine with phenylalanine at positions 4, 5 and 12 resulted in peptides with significant anti-fungal activity at pH 4.0 while peptide 113 is essentially devoid of anti-fungal activity at this lower pH. Similar modifications of the peptides of this invention, such as peptide 113, to allow the peptides to have anti-microbial activity at pFis other than neutral values are included in the present invention.
Peptide sequences containing permutations of the amino acid sequences of :native histatins and modified peptides can be produced by known methods, such as recombinant DNA
techniques and solid-phase synthesis. Cloned DNA encoding the human histatin;s may be obtained as described by Sabatini et aI. or Vanderspek et al.
(Sabatini. L.M. et a1.
(1989), Biochem. B:io~hys. Res. Comm. 160:495-502;
Vanderspek, J.C. .et a1. (1990), Arch. Oral Biol.
35(2):137-43). cDNA encoding the histatin-based peptides can be cloned by recombinant DNA techniques, for instance, by using degenerate oligonucleotides based on the amino acid sequence of the histatin-based peptides as primers for polymerase chain reaction amplification. Alternatively, oligonucleotides encoding histatins or histatin-based t CA 02223505 2001-02-26 peptides can be s5mthesized chemically using commercially available equipment. They can then be made double-stranded and cloned into vectors for amplification in prokaryotic or eukaryotic host cells .
Histatin-based peptides can be produced in a variety of expression vect:or/host systems, which are available commercially or can be reproduced according to recombinant DNA and cell culture techniques. The vector/host expression system:. can be prokaryotic or eucaryotic, and can include bacterial, yeast, insect, mammalian, and viral expression system;. The construction of expression vectors encoding histatin-based peptides, transfer of the vectors into various host cells, and production of peptides from transformed host cells can be accomplished using genetic engineering techniques, as described in manuals such as Molecular Cloning and Current Protocols in Molecular Biolocrv (Sambrook, J., Fritsch, E.F. and Maniatis, T.
(1989), Molecular Cloning, 2nd ed., Cold Spring Harbor Laboratory Press; Ausubel, F.M. et al., eds., Current Protocols in Molecular Biolocrv, New York; Greene Publishing Associates and Wiley-Interscience) Modified histatin based peptides, such as particular amino acid substitutions of peptide 113, can be synthesized chemically, or be produced from cloned DNAs containing mutated nucleotide: sequences. Histatin-based peptides encoded by expression vectors may be modified due to post-translational processing in a particular expression vector/host cell system. (See, e.g., Wold, F. (1981), Ann.
Rev. Biochem. 50:783-814). Histatin-based peptides may also be modified by chemical alteration of amino acid side-chain groups, or by other covalent modification.
(See, e.g., Glazer, A.N. et al. (1975), Chemical Modification of Proteins, North Holland; Katre, N.V. et a1.
(1987), Proc. Natl. Acad. Sci. USA 84:1487) Therapeutic Applications The histatin-based peptides of this inventian, . representing defined portions of the amino acid sequence of histatin-based peptide 113: peptide 113 itself, histatin 11, peptide 117, peptide 118, peptide 119, peptide 120 and peptide 129, and modified peptide 113 such as peptide 113-F4, peptide 113-F5, peptide 113-F12, peptide 113-F4.5, peptide 113-F4.5.12, peptide 113-K6, peptide 113-~H8, peptide 113-K6H8, peptide 113-F8, peptide 113-L4.5.12, peptide 113-Y4.5.12, peptide 113-Q2.10, peptide 7.13-Q3.9, can be used in compositions and methods of treatment for fungal, and in particular, candidal infection, or for bacterial infection. These methods of treatment for fungal or bacterial infection apply to preventive treatment as well. The compositions may contain combinations of histatin-based peptides, in order to obtain maximum activity against all developmental forms of the fungus.
The ionic strength, presence of various mono- and divalent ions, and pH of the compositions may be adjusted to obtain maximum anti-fungal or anti-bacterial activity of the histatin-based peptides, as described in Xu et a.7. (Xu, T.
et a1. (1991), Infect. Immun. 59(8):2549-54). Carriers appropriate for administration of anti-fungal agents to the vagina, the urethra, the ear, the oral cavity, the respiratory system, the ophthalmic region, various mucosal regions and skin are known, and described, for instance, in US 4,725,576 (Fundicidal Polyt~eptide Compositions Coza.taining L-His and Methods for Use Therefor by J.J.
Pollock and B.J. MacKay, February 16, 1988). Compositions for treatment of systemic infection can be administered by various routes, such as intravenously or subdermally.
Expression vectors encoding the above-mentioned peptides can be used in compositions and methods for anti-fungal or anti-bacterial treatment. Expression vectors may be administered in compositions which introduce genetic material encoding histatin-based peptides into cells of the patients. For example, recombinant expression vectors based on retroviruses or adenovirus vaccines may be used to infect patients.
A method of anti-fungal or anti-bacterial therapy using the above-described expression vectors is bacterial substitution therapy. Bacterial substitution therapy can be used to treat fungal or bacterial infection of areas in the urinary/reproductive, respiratory and/or gastro-intestinal tracts of a patient. The therapy comprises the following: 1) transforming a particular bacterium with DNA comprising an expression vector which encodes a histatin-based peptide described above, thereby producing transformed cells; 2) selecting transformed cells which express the peptide encoded by the expression vector, thereby obtaining transformed cells which express a histatin-based peptide; and 3) administering transformed cells which express a histatin-based peptide in an appropriate carrier to the infected area.
One application of bacterial substitution therapy is treatment of fungal or bacterial infections of the oral cavity. A number of species of the oral bacterial Streptococcus can be used as vehicles for the expression vectors. For example, recombinant S. lactis has been used in oral immunization of mice against a heterologous antigen. (Iwaki, M. et al. (1990), Infect. Immun.
58(9):2929-34). Other oral bacteria which can be used as vehicles for the expression vectors, plasmids for constructing expression vectors capable of amplification in oral bacterial host cells, transformation methods, and administration of compositions containing oral bacteria to humans have been described. (See, e.g., Kuramitsu, H.K. et a1. (1984), J. General Microbioloav 130:2497-2500; LeBlanc, D.J. et a1. (1978), Proc. Natl. Acad. Sci. USA
75(7):3484-3487; Macrina, F.L. et al. (1980), J. Bacteriolocry 143(3):1425-1435; Kuramitsu, H.K. et a1.
(1982), Infect. Immun. 36(1):435-436; Svanberg, M. et a1.
(1984), Infect. Immun. 43(3):817-821).
The compositions and methods for treatment of fungal or bacterial infections discussed above are not limited to use in humans, but can have veterinary applications as well.
Furthermore, the above-described compositions and methods for treatment of fungal infection can also be used for treatment of bacterial infections (e. g., of S. mutans, P. aeruginosa or P. gingivalis) and viral infections (e. g., of herpex simplex virus or human immunodeficiency virus type 1).
Hemaactlutination Activit,~r of Bacteria and Histatin Inhibition of this Activity Even though the association between hemagglutination activity and adherence on host cells in the oral environment is not clear, it is generally accepted that hemagglutination activity is an indicator for colonizing ability of bacteria. Periodontal pathogens must adhere to other bacteria and host cells in order to express their noxious destructive potential upon periodontal tissues.
Hemagglutinin is thought to be involved in bacterial colonization. Proteases such as collagenase, sialidase, and trypsin-like protease are involved in the degradation of host tissues, and low-molecular weight toxic products such as butyric and propionic acids which are cytotoxic are produced. In a similar fashion, B. forsythus has been shown to possess sialidase and trypsin-like protease.
Sialidase has the ability of altering the host response to periodontal microorganisms. For instance, it cleaves sialic acid from erythrocytes and leukocytes which results a.n removal of these cells from the circulation. It also decreases the ability of IgG to bind complement, decreases collagen production, and stimulates lymphocytes. Trypsin-.
like protease can cleave a variety of synthetic substrates.
It is so called because it can hydrolyse the synthetic substrate benzoyl-DL-arginine-naphylamide (BANA), used for detection of trypsin activity. P. gingivalis, B. forsythus and T. denticola possess strong BANA hydrolase activity.
B. forsythus also possesses hemagglutinin(s).
Ability for adherence on erythrocytes is of great importance in the interactions of periodontal pathogens with the host. The close proximity of these bacteria with the host tissues as well as with erythrocytes that bathe the periodontal pocket during progression of the disease, indicates multiple interrelations between these elements.
Additionally, periodontal microbes require heme-containing products for their survival and multiplication and this need dictates interactions with cells such as erythrocytes that are rich in these compounds. P. gingivalis has been shown to possess both hemagglutinin(s) and hemolysin that provide attachment on erythrocytes and utilization of heme-compounds.
It has been shown (Murakami et a1. (1990) Arch. Oral Biol. 9: 775) that histatin 5 and histatin 8 inhibit hemagglutination of P. gingivalis 381. Complete hemagglutination inhibition was reported for histatin 5 at a concentration of 5 nmole/ml. Thus, it appears that histatins and, more importantly, histatin-based peptides can play a role in inhibiting bacterial growth and deleterious activity in the periodontal region.
Clostripain Inhibition by Histatin-Based Peptides Clostripain is an endopeptidase enzyme synthesized by Clostridium histolyticum. This enzyme, with its protein degradative activity, can be inhibited by histatin 5 and by histatin-based peptides (see Table 1). Thus, histatin-based peptides can inhibit bacterial function by inhibiting bacterial enzymes which are essential for the bacterial viability.

A. Isolation and Chemical Svnthesis of Histatin-Based Peptides Isolation and. amino acid sequence determination of human histatins were performed as described in Oppenheim et a1.
(Oppenheim, F.G. e~t a1. (1988), J. Biol. Chem.
263(16):7472-7477). Human parotid secretion from healthy adults was stimulated using sour lemon candies, collected with Curby cups in ice-chilled graduated cylinders, pooled, dialyzed and lyophilized. Total protein in human parotid secretion was subjected to fractionation on Bio-Gel*P-2 (Bio-Rad Laboratories, Richmond, CA) developed in 0.05 M
ammonium formate buffer, pH 4Ø The protein fraction enriched with histatins was further purified using reversed-phase high-performance liquid chromatography on a C18 column. Purified histatins were evaporated to dryness, dissolved in deionixzed water, quantified by amino acid analysis, lyophilized, and stored at -20°C until use.
Histatin-based peptides were synthesized by the solid phase method of Merrifield. (Merrifield, B. (1986) Science 232:341-47). Peptides were synthesized by a MilliGen/Bioresearch Sam-Two Peptide Synthesizer using Fmoc L-amino acid kits (Millipore, Bedford, MA) and purified on a TSK ODS-i20T C18 column (5 Vim, 4.6 X 250 mm) using RP-HPLC (Pharmacia-LKB). The purified peptides were quantified by amino acid analysis on a Beckman System 6300 amino acid analyzer.
B. C. albicans K:illinQ
( 1 ) C. albi cans Svtock * Trademark A well-described strain of C. albicans was used in the bioassay. This strain, ATCC 44505, was originally isolated from the human oral cavity. Cultures were stored at 4°C on Sabouraud dextrose agar plates (Difco Laboratories, Detroit, MI) until use. Stationary phase growth cells were obtained following growth at 30°C for 18 h on Sabouraud dextrose agar plates. Colonies were harvested and suspended in 10 mM potassium phosphate buffer (PPB), pH

7.4.
To initiate log phase growth, an aliquot of stock C.
albicans was suspended in Sabouraud dextrose broth (Difco) and incubated at 30°C in a shaking water bath. The growth phase was determined by taking aliquots of the culture at one hour intervals to monitor the optical density (0.D.) at 560 nm. Early log phase was obtained at 4 to 6 h, indicated by an O.D. of about 0.6. Log phase cells were harvested and utilized in the blastoconidia killing assay in a manner identical to that described for stationary phase cells. A final concentration of105 cells/ml (either stationary or log phase fungus) was used in all assays.
(2) Suspension Buffers The standard suspension buffer utilized in the blastospore killing assay was 0.01 M PPB, pH 7.4. An alternate suspension buffer, N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acids (HEPES; Sigma Chemical Co., St.
Louis, MO), pH 7.4, can also be utilized.
(3) Bioassays The following assay was used to evaluate the effects of histatins on the killing of blastoconidia of C.
albicans.
a. For the killing of blastoconidia assay, 50 /.~1 aliquots of cells (2 x 105 cells/ml) diluted in suspension buffer were allowed to attach to a polystyrene 96-well micro-titer plate (COSTAR, Cambridge, MA) for 15 min at room temperature, and then incubated with an equal volume of a histatin or histatin peptide in suspension buffer for 1 h at 37°C. Controls were carried out in the absence of the histatin or histatin peptide.
After incubation, wells were washed three times by centrifugation at 1,OOOxg for 5 min and covered with aliquots of molten Sabouraud dextrose broth (Difco) containing 2% agarose (Sigma) at 45°C. The plate was then incubated at 30°C for 8 h. Under such conditions, live cells will divide and begin to form colonies, while dead cells will remain as single cells. To determine: the percentage of blastoconidia killed, a total of 100 single cells and/or colonies were counted under a Nikori inverted microscope at 400x magnification and the extent of killing was calculated using the formula: [1 - (number of colonies in treated sample)/(number of colonies in control)] x 100%.
(4) Statistical Analysis Data were obtained by calculating the mean and standard deviation from triplicate assays. From the dose response relationship, doses effecting a 50% killing (LDso) were determined.
C. BACTERIAL GROWTH INHIBITION AND CELL KILLING ASSAYS
(1) Bacterial Strains and Culture Conditions (a) The bacteria used in one investigation, Porphyromonas gingivalis strain A7A1-28, is a typical key pathogenic organism associated with destructive periodontal diseases. The bacteria were multiply * Trademark subcultured in Enriched Todd Hewitt broth (ETHB, Difco Lab., Detroit, MI). Microorganisms were stored in the same broths containing 20% and 50% glycerol, at -20°C
and -70°C, respectively. These served as stock cultures from which all preparations originated.
Working stock cultures were maintained by weekly transfer to Brain Heart Infusion Anaerobic Sheep Blood Agar plates (BHIA, Becton Dickinson and Co., Cockeysville, MD), and Trypticase Soy Anaerobic Sheep Blood Agar plates (TSA, Becton Dickinson and Co., Cockeysville, MD). Plates were incubated for 3 to 4 days under strictly anaerobic conditions. For the bacteriostatic assay, bacteria were collected from plates, inoculated into the aforementioned broth and grown at 37°C, under strictly anaerobic conditions for 24 to 48 hours.
(b) Two other bacterial species were used in a bacterial cell killing assay system. These bacterial species were Streptococcus mutans strain SJ32 and Pseudomonas aeruginosa ATCC Accession Number 27853.
The assays were performed using liquid overnight cultures (nutrient broth for P. aeruginosa; Todd Hewitt broth for S. mutans) growth media from frozen stocks of these bacterial species. In the assay, the bacteria were diluted into assay buffer (10 mM
Potassium Phosphate, pH 6.0 with 20 mM NaCl for P.
Aeruginosa; and 10 mM Potassium Phosphate, pH 5.2 with 20 mM NaCl for S.mutans) to a concentration of 2 x 105 cfu/ml (1 x 109 cfu/OD/ml) and combined with an equal volume .(250 ~,l) of peptide to produce 500 ~,1 incubation mixture with a final concentration of 105 cfu/ml. Controls constituted buffer and bacteria but no peptide. After incubation at 37°C (30 minutes incubation for P. aeruginosa; and 60 minutes incubation for S. mutans), the mixtures were plated WO 96!40768 PCT/LTS~96109374 onto agar media (nutrient agar for P. aerug~inosa; and Todd Hewitt media with 0.5% glucose for S. mutans) and incubated at 37°C until colonies developed. The mean number of colonies was determined from a minimum of 4 plates and percent killing was determined by comparing the colony number arising from control cultures versus the colony number arising from peptide-containing assay mixtures.
(2) Microdilution Bacteriostatic Assav A modification of the typical microdilution assay (Rotilie et al., 1975) for the determination of minimal inhibitory concentration (MIC) of antimicrobial agents was utilized to investigate the bacteriostatic activity of the peptides. A
standardized bacterial inoculum (P. girsgivalis) was exposed to serially diluted antimicrobial peptides in an enriched broth medium that was suitable for the growth of anaerobic bacteria. The test was adapted for use in the 96-well microtiter plates. Results with the microdilution method have been shown to be comparable to the other known techniques for antimicrobial susceptibility such as the dilution method, the agar dilution method, and the broth-disk elution method (Rosenblatt et al., 1979). In the typical assay, the microtiter plate was observed at multiple time points after incubation for visible growth. The modification introduced here was based on the spectrophotometric reading of the microtiter plate after incubation.
Microorganisms from cultures maintained in the aforementioned plates were inoculated into 5 ml of the above-mentioned broths and cultured overnight at 37°C
under strictly anaerobic conditions with continuous agitation on a minishaker (IKA-Labortechnik, Staufen i. Br., Germany). The bacteria were grown until reaching the late log phase and were then suspended in the same broths to an optical density (0.D.) of 0.1 at 560 nm. The peptides were diluted in 0.01 M phosphate buffered saline (PBS), pH 7. Forty ~C1 aliquots of peptide dilutions were added in each well of a U-bottom microtiter plate (Costar, Cambridge, MA) to give final concentrations of 2000, 1000, 500 and 250 ~.M. Twenty E.cl of bacterial inoculum was added to all the wells. Finally, 100 ~C1 of the suitable broth were added to each well. The optical density of the wells of the microtiter plate was determined using a microplate reader set at 550 nm and the plate was then incubated under strictly anaerobic conditions for 24 hours. Controls were made by replacing the peptide dilutions with PBS alone. After the incubation, the mixtures in each well were mixed manually to resuspend sedimented bacteria and the plate was read again. The experiments were conducted twice every time. The biologic activity was calculated according to the formula:
100-[[(Fin ODexp-In ODexp)/(Fin ODctr-In ODctr)] x 100]
where:
Fin ODexp is the OD of the final experimental group;
In ODexp is the OD of the initial experimental group;
Fin ODctr is the OD of the final control group; and In ODctr is the OD of the initial control group.
In addition, the % increase in time to reach mid-log phase growth was calculated.
The data presentation represent the means (tSEM) of at least 2 separate experiments.

WO 96/40768 PCT/US9~6/09374 D. INHIBITION OF HEMAGGLUTINATION ASSAYS
(1) Strains and Growth Conditions for Hemacralutination Assays The P. gingivalis strain of Section C.(:L) was also used for the hemagglutination assays. 'fhe bacterial growth and culture conditions were also the same as those described in Section C.(1).
(2) Hemagcrlutination Assay A classic assay was utilized to determine the hemagglutination potential of the P. gingival~s strain. Microorganisms were inoculated into BFB broth and cultured overnight, for approximately 24 hours at 37°C under strictly anaerobic conditions with continuous agitation on a minishaker (IKA-Labortechnik, Staufen i. Br., Germany). The bacteria were harvested by centrifugatin at 3,000 r.p.m. for 20 min, at 4°C, washed twice in 0.01 M phosphate buffered saline (PBS), pH 7.4, and suspended in the same buffer to an optical density of 1.0 at 550 nm. Erythrocytes were obtained from a young male with O-type blood, since no difference in hemagglutination was observed in preliminary experiments with different ABO blood groups. One ml of blood was drawn each timer washed twice in PBS at 1,000 r.p.m. for 10 min at 4°C and suspended in the same buffer at a 2% (v/v) final concentration. Fifty ~.1 of the bacterial suspension were serially diluted in PBS (two-fold steps) in a 96-well U-bottom microplate (Costar, Cambridge, MA).
Fifty ~.cl of the erythrocyte suspension were added to each well. Controls without bacteria or erythrocytes were included. The microplate was slightly shaken and incubated at room temperature for 2 hours. Visible examination on a white background was used to determine hemagglutination. The amount of hemagglutination was rated as none (-), moderate (+/-), or strong (+). Erythrocytes in control wells with PBS precipitated to the center of the well, whereas erythrocyte-bacteria aggregates precipitated at the periphery of the bottom. The hemagglutination titer was expressed as the reciprocal of the highest dilution of the bacterial suspension providing visible hemagglutination.
(3) Histatin Pet~tide Inhibition of Hemactcrlutination Assav Preparation of erythrocyte and bacterial suspensions were the same as for the hemagglutination assay. Fifty ~.1 of histatin peptide solutions were diluted in PBS in a U-bottom microplate, at various two-fold concentrations with 600 nmole/ml being the highest. The bacterial concentration utilized was normally twice the minimal concentration which gave strong hemagglutination. Equal volumes of the bacterial suspension were poured into the wells containing the histatin peptides. Finally, 50 ~1 of erythrocyte suspension were added in each well. The microplate was slightly shaken and incubated at room temperature for 2 hours. Controls were made by replacement of the peptide dilutions with PBS only.
The experiments were conducted at least twice. The lowest histatin peptide concentration without hemagglutination (complete inhibition) was determined upon visual examination. The highest final histatin peptide concentration utilized was 100 nmole/ml.
E. CLOSTRIPAIN ASSAYS
Clostripain from Clostridium histolyticum (Sigma Chemical Corp., St. Louis, MO) was dissolved in deionized water to a concenl~ration of 1 mg/mL (300 units/mg) and activated with them addition of 10 mmol/L DTT. To measure its hydrolytic aci=ivity, clostripain (6 units) was added to 50 nmol/L Hepes buffer, pH 7.5, containing 80 ~Cmol/L BAPNA
(benzoyl-arginine~-p-nitroanilide), together with 5.6 ~mol/L
of histatin peptide inhibitor. As controls, assays were performed in the absence of any histatin peptide inhibitor.
The activity was monitored continuously at 405 nm using a Molecular Devices* V,,,ax microtitre plate reader. The activities were determined from the maximum rates of substrate hydroly:;is. Assays were done in duplicate, and the means normali2:ed to the controls.
EXAMPLE 2. EFFECTS OF HISTATIN PEPTIDES ON FUNGAL OR
BAC.'TERIAL VIABILITY
Figures 2-11 and Table 1 summarize the results of the fungal killing, bacterial growth inhibition, bacterial cell killing, bacteria mediated hemagglutination inhibition and bacterial enzyme (clostripain) inhibition effects of histatin 5 and several tested histatin peptides. For comparison purposes, the anti-fungal and anti-bacterial effects of histatin 11 and the histatin-based peptides other than amino acid substituted variants of peptide 113 were assessed with synthesized histatin 5 as a standard.
The amino acid substituted variants of peptide 113 were assessed for their anti-fungal and anti-bacterial effects with peptide 113 as a standard.
Histatin 11 and histatin-based peptides 113, 117, 118, 119, 120 and 129 have C. albicans blastoconidia killing, P.
gingivalis growth inhibition, bacteria mediated hemagglutination inhibition and clostripain inhibition effects, The various modified peptide 113 variants, i.e.
peptide 113-F4, peptide 113-F5, peptide 113-F12, peptide 113-F4.5, peptide 113-F4.5.12, peptide 113-K6, peptide 113-* Trademark H8, peptide 113-K6H8, peptide 113-F8, peptide 113-L4.5.12, peptide 113-Y4.5.12, peptide 113-Q2.10, peptide 113-Q3.9, peptide 113 with an acetyl blocking group on the N-terminus of the peptide, and peptide 113 with a carbamyl blocking group on the N-terminus also exhibit anti-fungal and anti-bacterial activity. These antimicrobial effects are similar to those observed for histatin 5 and for histatin-based peptides 101-105. Although expected variations exist in anti-fungal and anti-bacterial effects between the tested peptides, the antimicrobial effects of the histatin-based peptides are comparable to those of histatin 5.
These results demonstrate that these histatin-based peptides are efficacious as anti-fungal or anti-bacterial agents. In particular, these results demonstrate that histatin-based peptide 113 and its subpeptides histatin 11, histatin-based peptides 117, 118, 119, 120 and 129 are more efficacious on the basis of molecular weight or amino acid sequence length than histatin 5. The anti-fungal and anti-bacterial activity of the histatins appears to be concentrated in the amino acid sequence of histatin-based peptide 113. Selected amino acid substitutions to form variants of peptide 113 also retain and often enhance the anti-fungal and anti-bacterial activity of the original peptide.

SUMMARY OF HISTATIN PEPTIDE SEQUENCES
AND BIOLOGICAL ACTIVITY TESTING
Histatin Peptide MW C. albicans P. c~incrivalis Hemacralut Clostrinain SynHis5 3037 3.O~.M 88.7% 3.12 ~,M <6.2 /1M

101 2025 3.5 1.56 7.5 102 10 +++

103 2613 4.5 56.6 1.56 19 105 2978 5.0 +++ 6.25 3.3 His 11 1080 + 100 NA

113 1563 5.8 34.95 25 25 117 1492 ++++

118 1426 ++++ 6.25 45 119 1279 ++ 25 >50 120 1151 ++ 50 >50 129 27 15.4 50 1g ~~y:
C. albicans: Results expressed as LDso (~.M) for % killing or %
killing at 50 ~.M dose (see below for scoring).
P. gingivalis: Results expressed as % increase in time to reach mid log-phase growth (0.2 OD) for the 2 mM dose or % inhibition at 500 ~.M
dose (see below for scoring).
Hemagglutination: Results expressed as lowest dose (~cM) at which hemagglutination was observed using P. Gingivalis.
Clostripain: Results expressed as ICso (~,M) for inhibition of Clostripain.
Scoring: The highest response seen was used in the scoring.
++++ 80 - 100%
+++ 50 - 80%
++ 20 - 50%
+ <20%
NA Not Active EQUIVALENTS
Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.

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(i) SEQUENCE CHARACTERISTICS: ' (A) LENGTH: 38 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE:
(A) NAME/KEY: Modified-site (B) LOCATION: 2 (D) OTHER INFORMATION: /note= "/product="PSE""
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:
Asp Xaa His Glu Lys Arg His His Gly Tyr Arg Arg Lys Phe His Glu AMENDED SHEET

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Lys His His Ser His Arg Glu Phe Pro Phe Tyr Gly Asp Tyr Gly Ser Asn Tyr Leu Tyr Asp Asn (2) INFORMATION FOR SEQ ID N0:2:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 27 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:2:
Arg Lys Phe His Glu Lys His His Ser His Arg Glu Phe Pro Phe Tyr Gly Asp Tyr Gly Ser Asn Tyr Leu Tyr Asp Asn 20 ' 25 (2) INFORMATION FOR SEQ ID N0:3:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 32 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:3:
Asp Ser His Ala Lys Arg His His Gly Tyr Lys Arg Lys Phe His G1u Lys His His Ser His Arg Gly Tyr Arg Ser Asn Tyr Leu Tyr Asp Asn (2) INFORMATION FOR SEQ.ID N0:4:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 21 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:4:
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(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO: S:
Asp Ser His Ala Lys Arg His His Gly Tyr Lys Arg Lys Phe His G1u Lys His His Ser His Arg Gly Tyr (2) INFORMATION FOR SEQ ID N0:6:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 25 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:6:
Asp Ser His A1a Lys Arg His His Gly Tyr Lys Arg Lys Phe His G1u Lys His His Ser His Arg G1y xyr Arg (2) INFORMATION FOR SEQ ID N0:7:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 13 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:7:
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(2) INFORMATION FOR SEQ ID NO: S:
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(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO: B:
Lys Phe His Glu Lys His His Ser His Arg Gly Tyr (2) INFORMATION FOR SEQ ID N0:9:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 14 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:9:
Arg Lys Phe his G1u Lys His His Ser His Arg G1y Tyr Arg (2) INFORMATION FOR SEQ ID NO:10:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 13 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10:
Lys Phe His Glu Lys His His Ser His Arg Gly Tyr Arg 1 s to (2) INFORMATION FOR SEQ ID NO:11:
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(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:12:
Lys Arg His His Gly Tyr Lys (2) INFORMATION FOR SEQ ID N0:13:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 21 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:13:
Lys Arg His His G1y Tyr Lys Arg Lys Phe His G1u Lys His His Ser His Arg Gly Tyr Arg (2) INFORMATION FOR SEQ ID N0:14:
(i) SEQUENCE CHARACTERISTICS:
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(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 18 amino acids (B)~ TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQ~JENCE DESCRIPTION: SEQ ID N0:15:
Lys Arg His His Gly Tyr Lys Arg Lys Phe His Glu Lys His His Ser His Arg (2) INFORMATION FOR SEQ ID N0:16:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 14 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLE~:ULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:16: , Gly Tyr Lys Arg Lys Phe His Glu Lys His His Ser His Arg (2) INFORMATION FOR SEQ ID N0:17:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 15 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
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(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:18:
Ala Lys Arg His His Gly Tyr Lys Arg Lys Phe His (2) INFORMATION FOR SEQ ID N0:19:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 11 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:19:
Lys Arg His His Gly Tyr Lys Arg Lys Phe His (2) INFORMATION FOR SEQ ID N0:20:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 11 amino acids (B) TYPE: amino acid (C) STRANDEDN~;SS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:20:
Ala Lys Arg His His Gly Tyr Lys Arg Lys Phe (2) INFORMATION FOR SEQ ID N0:21:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 10 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear AMEi~DED SHEEN

~ CA 02223505 1997-12-04 ..
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l~J.a Lys Arg His His Gly Tyr Lys Arg Lys 1 5 ~ 10 (2) INFORMATION FOR SEQ ID N0:22:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 9 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:22:
A1a Lys Arg His His Gly Tyr Lys Arg (2) INFORMATION FOR SEQ ID N0:23:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 10 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:23:
Lys Arg His His Gly Tyr Lys Arg Lys Phe (2) INFORMATION FOR SEQ ID N0:24:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:24:
Ala Lys Arg Phe His Gly Tyr Lys Arg Lys Phe His (2) INFORMATION FOR SEQ ID N0:25:
Ai~AENDED SHEET

~ CA 02223505 1997-12-04 ..,, ~' (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:25:
Ala Lys Arg His Phe Gly Tyr Lys Arg Lys Phe His (2) INFORMATION FOR SEQ ID N0:26:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 amino acids (B) TYPE: amino acid ' (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:26:
Ala Lys Arg His His Gly Tyr Lys Arg Lys Phe Phe (2) INFORMATION FOR SEQ ID N0:27:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE ~iYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:27:
Ala Lys Arg Phe Phe Gly Tyr Lys Arg Lys Phe His (2) INFORMATION FOR SEQ ID N0:28:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide AMENDED SHEET

.,. : ,.
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., " , (xi) SEQUENCE DESCRIPTION: SEQ ID N0:28:
Ala Lys Arg Phe Phe Gly Tyr Lys Arg Lys Phe Phe (2) INFORMATION FOR SEQ ID N0:29:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 amino acids ' (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:29:
Ala Lys Arg His His Lys Tyr Lys Arg Lys Phe His (2) INFORMATION FOR SEQ ID N0:30:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:30:
Ala Lys Arg His His Gly Tyr His Arg Lys Phe His (2) INFORMATION FOR SEQ ID N0:31:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:31:
Ala Lys Arg His His Lys Tyr His Arg Lys Phe His (2) INFORMATION FOR SEQ ID N0:32:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear A~IiEi~SnED S~~ET

;" ,' , _ , , , ' > , (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:32:
Ala Lys Arg His His Gly Tyr Phe Arg Lys Phe His 1 5 1 0.
(2) INFORMATION FOR SEQ ID N0:33:
(i) SEQUENCE CHARACTERISTICS: ' (A) LENGTH: 12 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:33:
A1a Lys Arg Leu Leu Gly Tyr Lys Arg Lys Phe Leu (2) INFORMATION FOR SEQ ID N0:34: °
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:34:
Ala Lys Arg Tyr Tyr Gly Tyr Lys Arg Lys Phe Tyr (2) INFORMATION FOR SEQ ID N0:35:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 amino acids (B; TYPE: amino acid (C) STRANDEDNESS:
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:35:
Ala Gln Arg His His G1y Tyr Lys Arg Gln Phe His (2) INFORMATION FOR SEQ ID N0:36:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
J~~AENDED SHEET

~ CA 02223505 1997-12-04 . "
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(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:36:
Ala Lys Gln His His Gly Tyr Lys Gln Lys Phe His ' (2) INFORMATION FOR SEQ ID N0:37: ' ' (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 amino acids (B) TYPE: amino acid (C) STRANDEDNESS:
° (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID N0:37:
Ala Gln Gln His His Gly Tyr Lys Gln Gln Phe His ° 1 s to AMENDED SHEET

Claims (12)

1. A composition for treating a fungal or bacterial infection comprising a substantially pure peptide and pharmaceutically acceptable excipients, wherein said substantially pure peptide has an amino acid sequence, of at least 8 amino acids, selected from th group consisting of:

a) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18;

b) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18 where the glycine at position 6 is replaced by lysine, arginine or another basic amino acid;

c) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18 where the lysine at position 8 is replaced by histidine, phenylalanine or another hydrophobic amino acid;

d) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18 where at least one of the histidines at positions 4, 5 and 12 is replaced by phenylalanine, tyrasine, leucine or another hydrophobic amino acid;

e) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18 where at least one of the lysines at positions 2 and 10 is replaced by glutamine, arginine or by another basic amino acid;

f) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18 where at least one of the arginines at positions 3 and 9 is replaced by glutamine, lysine or by another basic amino acid;
and g) any combination of the amino acid replacements of preceding sections b) - f) with the exception that glutamine or any other non-basic amino acid cannot simultaneously occupy positions 2, 3, 9 and 10 of the amino acid sequence.

2. A composition of Claim 1 wherein said substantially pure peptide is selected from the group consisting of:
of:

a) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18;

b) the amino acid sequence of histatin 11 as set forth in SEQ ID NO: 11;

c) the amino acid sequence of peptide 129 as set forth in SEQ ID NO: 23;

d) the amino acid sequence of peptide 117 as set forth in SEQ ID NO: 19;

e) the amino acid sequence of peptide 118 as set forth in SEQ ID NO: 20;

f) the amino acid sequence of peptide 119 as set forth in SEQ ID NO: 21;

g) the amino acid sequence of peptide 120 as set forth in SEQ.ID NO: 22;

h) the amino acid sequence of peptide 113-F4 as set forth in SEQ ID NO: 24;

i) the amino acid sequence of peptide 113-F5 as set forth in SEQ ID NO: 25;

j) the amino acid sequence of peptide 113-F12 as set forth in SEQ ID NO: 26;

k) the amino acid sequence of peptide 113-F4.5 as set forth in SEQ ID NO: 27;

l) the amino acid sequence of peptide 113-F4.5.12 as set forth in SEQ ID NO: 28;

m) the amino acid sequence of peptide 113-K6 as set forth in SEQ ID NO: 29;

n) the amino acid sequence of peptide 113-H8 as set forth in SEQ ID NO: 30;

o) the amino acid sequence of peptide 113-K6H8 as set forth in SEQ ID NO: 31;

p) the amino acid sequence of peptide 113-F8 as set forth in SEQ ID NO: 32 q) the amino acid sequence of peptide 113-L4.5.12 as set forth in SEQ ID NO: 33;

r) the amino acid sequence of peptide 113-Y4.5.12 as set forth in SEQ ID NO: 34;

s) the amino acid sequence of peptide 113-Q2.10 as set forth in SEQ ID NO: 35;

t) the amino acid sequence of peptide 113-Q3.9 as set forth in SEQ ID NO: 36;and u) combinations of two or more of the above with the exception of peptide 113-Q2.3.9.10 as set forth in SEQ ID NO: 37.

3. A composition of Claim 1 wherein the peptide has at least one substituent addition selected from the group consisting of a natural or modified amino acid addition to the N-terminus of said peptide, a natural or modified amino acid addition to the C-terminus of said peptide, an acetyl addition to the N-terminal amino acid, a carbamyl addition to the N-terminal amino-acid, and an amide addition to the C-terminal amino acid.

4. Substantially pure peptide having an amino acid sequence, of at least 8 amino acids, selected from the group consisting of:

a) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18.

b) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18 where the glycine at position 6 is replaced by lysine, arginine or another basic amino acid;
c) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18 where the lysine at position 8 is replaced by histidine, phenylalanine or another hydrophobic amino acid;

d) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18 where at least one of the histidines at positions 4, 5 and 12 is replaced by phenylalanine, tyrosine, leucine or another hydrophobic amino acid;
e) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18 where at least one of the lysines at positions 2 and 10 is replaced by glutamine, arginine or by another basic amino acid;
f) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18 where at least one of the arginines at positions 3 and 9 is replaced by glutamine, lysine or by another basic amino acid;
and g) any combination of the amino acid replacements of preceding sections b) - f) with the exception that glutamine or any other non-basic amino acid cannot simultaneously occupy positions 2, 3, 9 and 10 of the amino acid sequence.

5. Substantially pure peptide of Claim 4 selected from the group consisting of:

a) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18;

b) the amino acid sequence of histidine 11 as set forth in SEQ ID NO: 11;

c) the amino acid sequence of peptide 129 as set forth in SEQ ID NO: 23;

d) the amino acid sequence of peptide 117 as set forth in SEQ ID NO: 19;

e) the amino acid sequence of peptide 118 as set forth in SEQ ID NO: 20;

f) the amino acid sequence of peptide 119 as set forth in SEQ ID NO: 21;

g) the amino acid sequence of peptide 120 as set acid sequence forth in SEQ ID NO: 22;

h) the amino acid sequence of peptide 113-F4 as set forth in SEQ ID NO: 24;

i) the amino acid sequence of peptide 113-F5 as set forth in SEQ ID NO: 25;

j) the amino acid sequence of peptide 113-F12 as set forth in SEQ ID NO: 26;

k) the amino acid sequence of peptide 113-F4.5 as set forth in SEQ ID NO: 27;

1) the amino acid sequence of peptide 113-F4.5.12 as set forth in SEQ ID NO: 28;

m) the amino acid sequence of peptide 113-K6 as set forth in SEQ ID NO: 29;

n) the amino acid sequence of peptide 113-H8 as set forth in SEQ ID NO: 30;

o) the amino acid sequence of peptide 113-K6H8 as set forth in SEQ ID NO: 31;

p) the amino acid sequence of peptide 113-F8 as set forth in SEQ ID NO: 32;

q) the amino acid sequence of peptide 113-L4.5.12 as set forth in SEQ ID NO: 33;

r) the amino acid sequence of peptide 113-Y4.5.12 as set forth in SEQ ID NO: 34;

s) the amino acid sequence of peptide 113-Q2.10 as set forth in SEQ ID NO: 35; and t) the amino acid sequence of peptide 113-Q3.9 as set forth in SEQ ID NO: 36.

6. A substantially pure peptide of Claim 4 wherein the peptide has at least one substituent addition selected from the group consisting of a natural or modified amino acid addition to the N-terminus of said peptide, a natural or modified amino acid addition to the C-terminus of said peptide, an acetyl addition to the N-terminal amino acid, a carbamyl addition to the N-terminal amino-acid, and an amide addition to the C-terminal amino acid.

7 . A use of a therapeutically effective amount of at least one peptide having an amino acid sequence, of at least 8 amino acids, selected from the group consisting of:
a) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18;
b) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18 where the glycine at position 6 is replaced by lysine, arginine or another basic amino acid;
c) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18 where the lysine at position 8 is replaced by histidine, phenylalanine or another hydrophobic amino acid;
d) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18 where at least one of the histidines at positions 4, 5 and 12 is replaced by phenylalanine, tyrosine, leucine or another hydrophobic amino acid;
e) the amino acid sequence of peptide 113 as set forth in SEQ.ID NO: 18 where at least one of the lysines at positions 2 and 10 is replaced by glutamine, arginine or by another basic amino acid;
f) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18 where at least one of the arginines at positions 3 and 9 is replaced by glutamine, lysine or by another basic amino acid;

g) any combination of the amino acid replacements of preceding sections b) - f) with the exception that glutamine or any other non-basic amino acid cannot simultaneously occupy positions 2, 3, 9 and 10 of the amino acid sequence;
for treating a fungal or bacterial infection in an individual.

8 . A use of a therapeutically effective amount of at least one peptide having an amino acid sequence, of at least 8 amino acids, selected from the group consisting of:
a) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18;
b) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18 where the glycine at position 6 is replaced by lysine, arginine or another basic amino acid;
c) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18 where the lysine at position 8 is replaced by histidine, phenylalanine or another hydrophobic amino acid;
d) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18 where at least one of the histidines at positions 4, 5 and 12 is replaced by phenylalanine, tyrosine, leucine or another hydrophobic amino acid;

e) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18 where at least one of the lysines at positions 2 and 10 is replaced by glutamine, arginine or by another basic amino acid;
f) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18 where at least one of the arginines at positions 3 and 9 is replaced by glutamine, lysine or by another basic amino acid;
g) any combination of the amino acid replacements of preceding sections b) - f) with the exception that glutamine or any other non-basic amino acid cannot simultaneously occupy positions 2, 3, 9 and 10 of the amino acid sequence;
for the production of a medicament for treating a fungal or bacterial infection in an individual.

9. The use of Claim 7 or 8 wherein said peptide is selected from the group consisting of:
a) the amino acid sequence of peptide 113 as set forth in SEQ ID NO: 18;
b) the-amino acid sequence of histatin 11 as set forth in SEQ ID NO: 11;
c) the amino acid sequence of peptide 129 as set forth in SEQ ID NO: 23;
d) the amino acid sequence of peptide 117 as set forth in SEQ ID NO: 19;
e) the amino acid sequence of peptide 118 as set forth in SEQ ID NO: 20;
f) the amino acid sequence of peptide 119 as set forth in SEQ ID NO: 21;
g) the amino acid sequence of peptide 120 as set forth in SEQ ID NO: 22.
h) the amino acid sequence of peptide 113-F4 as set forth in SEQ ID NO: 24;
i) the amino acid sequence of peptide 113-F5 as set forth in SEQ ID NO: 25;

j) the amino acid sequence of peptide 113-F12 as set forth in SEQ ID NO: 26;
k) the amino acid sequence of peptide 113-F4.5 as set forth in SEQ ID NO: 27;
l) the amino acid sequence of peptide 113-F4.5.12 as set forth in SEQ ID NO: 28;
m) the amino acid sequence of peptide 113-K6 as set forth in SEQ ID NO: 29;
n) the amino acid sequence of peptide 113-H8 as set forth in SEQ ID NO: 30;
o) the amino acid sequence of peptide 113-K6H8 as set forth in SEQ ID NO: 31;
p) the amino acid sequence of peptide 113-F8 as set forth in SEQ ID NO: 32;
q) the amino acid sequence of peptide 113-L4.5.12 as set forth in SEQ ID NO: 33;
r) the amino acid sequence of peptide 113-Y4.5.12 as set forth in SEQ ID NO: 34;
s) the amino acid sequence of peptide 113-Q2.10 as set forth in SEQ ID NO; 35;and t) the amino acid sequence of peptide 113-Q3.9 as set forth in SEQ ID NO: 36.

10. The use of claim 7 or 8 wherein the peptide has at least one substituent addition selected from the group consisting of a natural or modified amino acid addition to the N-terminus of said peptide, a natural or modified amino acid addition to the C-terminus of said peptide, an acetyl addition to the N-terminal amino acid, a carbamyl addition to the N-terminal amino-acid, and an amide addition to the C-terminal amino acid.

11. The use of Claim 7 or 8 wherein said fungal or bacterial infection is selected from the group consisting of:
a) an infection of the oral cavity;
b) an infection of the vagina;
c) an infection of the urethra;
d) an infection of the ear;
e) an infection of the skin;
f) a respiratory infection;
g) a mucosal infection;
h) an ophthalmic infection; and i) a systemic infection.

12. The use of Claim 11 wherein the fungus or bacterium is selected from the group consisting of:
a) Candida albicans;
b) Actinomyces actinomycetemcomitans;
c) Actinomyces viscosus;
d) Bacteroides forsythus;
e) Bacteriodes fragilis;
f) Bacteriodes gracilis;
g) Bacteriodes ureolyticus;
h) Campylobacter concisus;
i) Campylobacter rectus;
j) Campylobacter showae;
k) Campylobacter sputorum;
l) Capnocytophaga gingivalis;
m) Capnocytophaga ochracea;
n) Capnocytophaga sputigena;
o) Clostridium histolyticum;
p) Eikenella corrodens;
q) Eubacterium nodatum;

r) Fusobacterium nucleatum;
s) Fusobacterium periodonticum;
t) Peptostreptococcus micros;
u) Porphyromonas endodontalis;
v) Porphyromonas gingivalis;
w) Prevotella intermedia;
x) Prevotella nigrescens;
y) Propionibacterium acnes;
z) Pseudomonas aeruginosa;
aa) Selenomonas noxia;
bb) Staphylococcus aureus;
cc) Streptococcus constellatus;
dd) Streptococcus gordonii;
ee) Streptococcus intermedius;
ff) Streptococcus mutans;
gg) Streptococcus oralis;
hh) Streptococcus pneumonia;
ii) Streptococcus sanguis;
kk) Treponema denticola;
ll) Treponema pectinovorum;
mm) Treponema socranskii;
nn) Veillonella parvula; and oo) Wolinella succinogenes.