CA2225390A1 - Silicon-based sleeve devices for chemical reactions - Google Patents

Silicon-based sleeve devices for chemical reactions

Info

Publication number
CA2225390A1
CA2225390A1 CA002225390A CA2225390A CA2225390A1 CA 2225390 A1 CA2225390 A1 CA 2225390A1 CA 002225390 A CA002225390 A CA 002225390A CA 2225390 A CA2225390 A CA 2225390A CA 2225390 A1 CA2225390 A1 CA 2225390A1
Authority
CA
Canada
Prior art keywords
reaction
silicon
reaction chamber
synthesis
combines
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002225390A
Other languages
French (fr)
Other versions
CA2225390C (en
Inventor
M. Allen Northrup
Raymond P. Mariella, Jr.
Anthony V. Carrano
Joseph W. Balch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
Original Assignee
The Regents Of The University Of California
M. Allen Northrup
Raymond P. Mariella, Jr.
Anthony V. Carrano
Joseph W. Balch
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Regents Of The University Of California, M. Allen Northrup, Raymond P. Mariella, Jr., Anthony V. Carrano, Joseph W. Balch filed Critical The Regents Of The University Of California
Publication of CA2225390A1 publication Critical patent/CA2225390A1/en
Application granted granted Critical
Publication of CA2225390C publication Critical patent/CA2225390C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/66—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light electrically excited, e.g. electroluminescence
    • G01N21/69—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light electrically excited, e.g. electroluminescence specially adapted for fluids, e.g. molten metal
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0332—Cuvette constructions with temperature control
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00819—Materials of construction
    • B01J2219/00824—Ceramic
    • B01J2219/00828—Silicon wafers or plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00851—Additional features
    • B01J2219/00853—Employing electrode arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00873—Heat exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00889—Mixing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00891—Feeding or evacuation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00905—Separation
    • B01J2219/00912—Separation by electrophoresis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00925—Irradiation
    • B01J2219/00934—Electromagnetic waves
    • B01J2219/00936—UV-radiations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00925—Irradiation
    • B01J2219/00934—Electromagnetic waves
    • B01J2219/00945—Infra-red light
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/0095—Control aspects
    • B01J2219/00952—Sensing operations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B81—MICROSTRUCTURAL TECHNOLOGY
    • B81C—PROCESSES OR APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OR TREATMENT OF MICROSTRUCTURAL DEVICES OR SYSTEMS
    • B81C3/00—Assembling of devices or systems from individually processed components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0346—Capillary cells; Microcells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Plasma & Fusion (AREA)
  • Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

A silicon-based sleeve type chemical reaction chamber (41) that combines heaters, such as doped polysilicon for heating, and bulk silicon for convection cooling. The reaction chamber combines a critical ratio of silicon and silicon nitride to the volume of material to be heated (e.g., a liquid) in order to provide uniform heating, yet low power requirements. The reaction chamber will also allow the introduction of a secondary tube (45) (e.g., plastic) into the reaction sleeve that contains the reaction mixture thereby alleviating any potential materials incompatibility issues. The reaction chamber may be utilized in any chemical reaction system for synthesis or processing of organic, inorganic, or biochemical reactions, such as the polymerase chain reaction (PCR) and/or other DNA reactions, such as the ligase chain reaction, which are examples of a synthetic, thermal-cycling-based reaction.
The reaction chamber may be used in synthesis instruments, particularly those for DNA amplification and synthesis.
CA002225390A 1995-06-20 1996-06-17 Silicon-based sleeve devices for chemical reactions Expired - Lifetime CA2225390C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/492,678 US5589136A (en) 1995-06-20 1995-06-20 Silicon-based sleeve devices for chemical reactions
US08/492,678 1995-06-20
PCT/US1996/010453 WO1997000726A1 (en) 1995-06-20 1996-06-17 Silicon-based sleeve devices for chemical reactions

Publications (2)

Publication Number Publication Date
CA2225390A1 true CA2225390A1 (en) 1997-01-09
CA2225390C CA2225390C (en) 2008-09-30

Family

ID=23957196

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002225390A Expired - Lifetime CA2225390C (en) 1995-06-20 1996-06-17 Silicon-based sleeve devices for chemical reactions

Country Status (7)

Country Link
US (1) US5589136A (en)
EP (1) EP0871545B1 (en)
JP (1) JP4091656B2 (en)
CA (1) CA2225390C (en)
DE (1) DE69634175T2 (en)
ES (1) ES2236739T3 (en)
WO (1) WO1997000726A1 (en)

Families Citing this family (306)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US20030017081A1 (en) * 1994-02-10 2003-01-23 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
US6287850B1 (en) * 1995-06-07 2001-09-11 Affymetrix, Inc. Bioarray chip reaction apparatus and its manufacture
US5814565A (en) * 1995-02-23 1998-09-29 University Of Utah Research Foundation Integrated optic waveguide immunosensor
US6207369B1 (en) * 1995-03-10 2001-03-27 Meso Scale Technologies, Llc Multi-array, multi-specific electrochemiluminescence testing
US6428955B1 (en) 1995-03-17 2002-08-06 Sequenom, Inc. DNA diagnostics based on mass spectrometry
US5830655A (en) 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US6524532B1 (en) * 1995-06-20 2003-02-25 The Regents Of The University Of California Microfabricated sleeve devices for chemical reactions
US5849208A (en) * 1995-09-07 1998-12-15 Microfab Technoologies, Inc. Making apparatus for conducting biochemical analyses
US6048734A (en) 1995-09-15 2000-04-11 The Regents Of The University Of Michigan Thermal microvalves in a fluid flow method
US6051378A (en) 1996-03-04 2000-04-18 Genetrace Systems Inc. Methods of screening nucleic acids using mass spectrometry
US5885470A (en) 1997-04-14 1999-03-23 Caliper Technologies Corporation Controlled fluid transport in microfabricated polymeric substrates
US6054277A (en) * 1996-05-08 2000-04-25 Regents Of The University Of Minnesota Integrated microchip genetic testing system
US5919364A (en) * 1996-06-24 1999-07-06 Regents Of The University Of California Microfabricated filter and shell constructed with a permeable membrane
GB9616540D0 (en) * 1996-08-06 1996-09-25 Cavendish Kinetics Ltd Integrated circuit device manufacture
GB9618595D0 (en) * 1996-09-06 1996-10-16 Central Research Lab Ltd Reaction cell
US5965363A (en) 1996-09-19 1999-10-12 Genetrace Systems Inc. Methods of preparing nucleic acids for mass spectrometric analysis
US6221654B1 (en) 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
US5922285A (en) * 1996-10-01 1999-07-13 Texas Instruments Incorporated Integrated fluorescence-based biochemical sensor
US7285422B1 (en) 1997-01-23 2007-10-23 Sequenom, Inc. Systems and methods for preparing and analyzing low volume analyte array elements
ATE259056T1 (en) 1996-11-06 2004-02-15 Sequenom Inc METHOD FOR MASS SPECTROMETRY
US6024925A (en) 1997-01-23 2000-02-15 Sequenom, Inc. Systems and methods for preparing low volume analyte array elements
US6379929B1 (en) 1996-11-20 2002-04-30 The Regents Of The University Of Michigan Chip-based isothermal amplification devices and methods
US5804384A (en) * 1996-12-06 1998-09-08 Vysis, Inc. Devices and methods for detecting multiple analytes in samples
JP2001524808A (en) 1996-12-10 2001-12-04 ジーントレイス・システムズ・インコーポレイテッド Releasable non-volatile mass labeling molecules
US8293064B2 (en) * 1998-03-02 2012-10-23 Cepheid Method for fabricating a reaction vessel
AU6343398A (en) * 1997-02-28 1998-09-18 Cepheid Heat exchanging, optically interrogated chemical reaction assembly
US5958349A (en) * 1997-02-28 1999-09-28 Cepheid Reaction vessel for heat-exchanging chemical processes
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
WO1998050773A2 (en) * 1997-05-08 1998-11-12 University Of Minnesota Microcantilever biosensor
JP3654481B2 (en) * 1997-06-05 2005-06-02 独立行政法人理化学研究所 Microreactor for biochemical reaction
US6469311B1 (en) 1997-07-16 2002-10-22 Molecular Devices Corporation Detection device for light transmitted from a sensed volume
US6258326B1 (en) 1997-09-20 2001-07-10 Ljl Biosystems, Inc. Sample holders with reference fiducials
US6097025A (en) * 1997-10-31 2000-08-01 Ljl Biosystems, Inc. Light detection device having an optical-path switching mechanism
US6071748A (en) 1997-07-16 2000-06-06 Ljl Biosystems, Inc. Light detection device
US5965410A (en) 1997-09-02 1999-10-12 Caliper Technologies Corp. Electrical current for controlling fluid parameters in microchannels
US6413783B1 (en) * 1997-09-18 2002-07-02 Meso Scale Technologies, Llc Assay sonication apparatus and methodology
US6576476B1 (en) 1998-09-02 2003-06-10 Ljl Biosystems, Inc. Chemiluminescence detection method and device
US6825921B1 (en) 1999-11-10 2004-11-30 Molecular Devices Corporation Multi-mode light detection system
WO2000050877A1 (en) 1999-02-23 2000-08-31 Ljl Biosystems, Inc. Frequency-domain light detection device
US6297018B1 (en) 1998-04-17 2001-10-02 Ljl Biosystems, Inc. Methods and apparatus for detecting nucleic acid polymorphisms
US6326605B1 (en) 1998-02-20 2001-12-04 Ljl Biosystems, Inc. Broad range light detection system
US6540895B1 (en) 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
US7214298B2 (en) 1997-09-23 2007-05-08 California Institute Of Technology Microfabricated cell sorter
US6251660B1 (en) 1997-11-25 2001-06-26 Mosaic Technologies, Inc. Devices and methods for detecting target molecules in biological samples
US6174675B1 (en) 1997-11-25 2001-01-16 Caliper Technologies Corp. Electrical current for controlling fluid parameters in microchannels
US6268131B1 (en) 1997-12-15 2001-07-31 Sequenom, Inc. Mass spectrometric methods for sequencing nucleic acids
EP1179585B1 (en) * 1997-12-24 2008-07-09 Cepheid Device and method for lysis
US6210882B1 (en) 1998-01-29 2001-04-03 Mayo Foundation For Medical Education And Reseach Rapid thermocycling for sample analysis
US6369893B1 (en) 1998-05-19 2002-04-09 Cepheid Multi-channel optical detection system
US6660228B1 (en) 1998-03-02 2003-12-09 Cepheid Apparatus for performing heat-exchanging, chemical reactions
AU764319B2 (en) * 1998-03-17 2003-08-14 Cepheid Chemical processing device
US6979424B2 (en) 1998-03-17 2005-12-27 Cepheid Integrated sample analysis device
US7188001B2 (en) * 1998-03-23 2007-03-06 Cepheid System and method for temperature control
EP1066551A2 (en) * 1998-03-23 2001-01-10 Cepheid Multi-site reactor system with dynamic, independent control of individual reaction sites
US6537751B1 (en) * 1998-04-21 2003-03-25 Genset S.A. Biallelic markers for use in constructing a high density disequilibrium map of the human genome
US6723564B2 (en) 1998-05-07 2004-04-20 Sequenom, Inc. IR MALDI mass spectrometry of nucleic acids using liquid matrices
US6200531B1 (en) * 1998-05-11 2001-03-13 Igen International, Inc. Apparatus for carrying out electrochemiluminescence test measurements
EP1080364B1 (en) * 1998-05-19 2002-11-20 Cepheid Multi-channel optical detection system
AU5667599A (en) 1998-07-27 2000-02-21 Ljl Biosystems, Inc. Apparatus and methods for time-resolved spectroscopic measurements
AU5223899A (en) 1998-07-27 2000-02-21 Ljl Biosystems, Inc. Apparatus and methods for spectroscopic measurements
JP2002522065A (en) * 1998-08-10 2002-07-23 ジェノミック ソリューションズ インコーポレイテッド Heat and fluid circulation device for nucleic acid hybridization
US6638716B2 (en) 1998-08-24 2003-10-28 Therasense, Inc. Rapid amperometric verification of PCR amplification of DNA
US6281006B1 (en) 1998-08-24 2001-08-28 Therasense, Inc. Electrochemical affinity assay
PT1108207E (en) * 1998-08-26 2008-08-06 Sensors For Med & Science Inc Optical-based sensing devices
WO2000017401A2 (en) * 1998-09-21 2000-03-30 Ramot University Authority For Applied Research & Industrial Development Ltd. Method and apparatus for effecting and monitoring nucleic acid amplification
US6572830B1 (en) 1998-10-09 2003-06-03 Motorola, Inc. Integrated multilayered microfludic devices and methods for making the same
US6413780B1 (en) * 1998-10-14 2002-07-02 Abbott Laboratories Structure and method for performing a determination of an item of interest in a sample
DE69913721T2 (en) * 1998-10-16 2004-10-28 Commissariat à l'Energie Atomique TEST CARRIER FOR CHEMICAL AND / OR BIOCHEMICAL ANALYSIS
US6503750B1 (en) * 1998-11-25 2003-01-07 The Regents Of The University Of California PCR thermocycler
US7150994B2 (en) * 1999-03-03 2006-12-19 Symyx Technologies, Inc. Parallel flow process optimization reactor
US20040053290A1 (en) * 2000-01-11 2004-03-18 Terbrueggen Robert Henry Devices and methods for biochip multiplexing
FR2795426A1 (en) * 1999-06-22 2000-12-29 Commissariat Energie Atomique Support for genetic analysis comprising reservoir(s) for a medium to be analyzed connected by passage(s) having temperature control device(s) to a test strip with analysis sites having biological probes
CA2379927A1 (en) * 1999-07-28 2001-02-01 Genset S.A. Integration of biochemical protocols in a continuous flow microfluidic device
US6932951B1 (en) 1999-10-29 2005-08-23 Massachusetts Institute Of Technology Microfabricated chemical reactor
US6642046B1 (en) 1999-12-09 2003-11-04 Motorola, Inc. Method and apparatus for performing biological reactions on a substrate surface
JP2003517591A (en) * 1999-12-09 2003-05-27 モトローラ・インコーポレイテッド Multilayer microfluidic device for reaction of analytical samples
WO2001046458A1 (en) * 1999-12-20 2001-06-28 The Penn State Research Foundation Deposited thin films and their use in detection, attachment, and bio-medical applications
US6699713B2 (en) * 2000-01-04 2004-03-02 The Regents Of The University Of California Polymerase chain reaction system
EP1738829A1 (en) * 2000-01-04 2007-01-03 The Regents of the University of California Polymerase chain reaction system
US6403037B1 (en) * 2000-02-04 2002-06-11 Cepheid Reaction vessel and temperature control system
DE10006214A1 (en) 2000-02-11 2001-08-16 Roche Diagnostics Gmbh System for simple nucleic acid analysis
US6824669B1 (en) 2000-02-17 2004-11-30 Motorola, Inc. Protein and peptide sensors using electrical detection methods
WO2001067369A2 (en) * 2000-03-03 2001-09-13 California Institute Of Technology Combinatorial array for nucleic acid analysis
ATE287291T1 (en) 2000-03-07 2005-02-15 Symyx Technologies Inc PROCESS OPTIMIZATION REACTOR WITH PARALLEL FLOW
US6927851B2 (en) 2000-03-31 2005-08-09 Neogen Corporation Methods and apparatus to improve the sensitivity and reproducibility of bioluminescent analytical methods
US6653147B2 (en) 2000-03-31 2003-11-25 Neogen Corporation Apparatus and method for chemiluminescent assays
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
ATE423221T1 (en) 2000-06-13 2009-03-15 Univ Boston USE OF MASS-MATCHED NUCLEOTIDES IN THE ANALYSIS OF OLIGONUCLEOTIDE MIXTURES AND IN HIGH-MULTIPLEX NUCLEIC ACID SEQUENCING
AU2001273057A1 (en) 2000-06-27 2002-01-08 Fluidigm Corporation A microfluidic design automation method and system
US6563581B1 (en) * 2000-07-14 2003-05-13 Applera Corporation Scanning system and method for scanning a plurality of samples
US6600558B2 (en) * 2000-08-22 2003-07-29 Nippon Telegraph And Telephone Corporation Micro-fluidic cell for optical detection of gases and method for producing same
EP1336097A4 (en) 2000-10-13 2006-02-01 Fluidigm Corp Microfluidic device based sample injection system for analytical devices
US20020142483A1 (en) 2000-10-30 2002-10-03 Sequenom, Inc. Method and apparatus for delivery of submicroliter volumes onto a substrate
US20020132360A1 (en) 2000-11-17 2002-09-19 Flir Systems Boston, Inc. Apparatus and methods for infrared calorimetric measurements
WO2002061858A2 (en) 2000-11-17 2002-08-08 Thermogenic Imaging, Inc. Apparatus and methods for infrared calorimetric measurements
WO2002059625A2 (en) * 2000-12-26 2002-08-01 Weigl Bernhard H Microfluidic cartridge with integrated electronics
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US6509186B1 (en) 2001-02-16 2003-01-21 Institute Of Microelectronics Miniaturized thermal cycler
US6432695B1 (en) 2001-02-16 2002-08-13 Institute Of Microelectronics Miniaturized thermal cycler
US20050196785A1 (en) * 2001-03-05 2005-09-08 California Institute Of Technology Combinational array for nucleic acid analysis
US7118917B2 (en) 2001-03-07 2006-10-10 Symyx Technologies, Inc. Parallel flow reactor having improved thermal control
US6586233B2 (en) 2001-03-09 2003-07-01 The Regents Of The University Of California Convectively driven PCR thermal-cycling
US7192557B2 (en) 2001-03-28 2007-03-20 Handylab, Inc. Methods and systems for releasing intracellular material from cells within microfluidic samples of fluids
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US6575188B2 (en) 2001-07-26 2003-06-10 Handylab, Inc. Methods and systems for fluid control in microfluidic devices
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US7323140B2 (en) 2001-03-28 2008-01-29 Handylab, Inc. Moving microdroplets in a microfluidic device
US7270786B2 (en) 2001-03-28 2007-09-18 Handylab, Inc. Methods and systems for processing microfluidic samples of particle containing fluids
US6960437B2 (en) 2001-04-06 2005-11-01 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
JP2004534226A (en) 2001-06-29 2004-11-11 メソ スケイル テクノロジーズ，エルエルシー Assay plate, reader system and method for luminescence test measurement
US6923939B1 (en) * 2001-07-05 2005-08-02 Uop Llc Heat activated membrane introduction apparatus and method for screening materials
US20030032172A1 (en) * 2001-07-06 2003-02-13 The Regents Of The University Of California Automated nucleic acid assay system
DK1412487T3 (en) 2001-07-30 2010-08-30 Meso Scale Technologies Llc Assay electrodes having immobilized lipid / protein layers and methods for preparing and using these
US6939632B2 (en) * 2001-08-06 2005-09-06 Massachusetts Institute Of Technology Thermally efficient micromachined device
EP1423500A4 (en) * 2001-08-06 2008-07-16 Univ Vanderbilt Device and methods for detecting the response of a plurality of cells to at least one analyte of interest
WO2003019158A2 (en) * 2001-08-21 2003-03-06 Bestmann, Lukas Thermo-optical analysis system for biochemical reactions
US7858321B2 (en) 2001-09-10 2010-12-28 Meso Scale Technologies, Llc Methods and apparatus for conducting multiple measurements on a sample
KR100488281B1 (en) * 2001-09-15 2005-05-10 아람 바이오시스템 주식회사 Method and apparatus for amplification of nucleic acid sequences by using thermal convection
US8440093B1 (en) 2001-10-26 2013-05-14 Fuidigm Corporation Methods and devices for electronic and magnetic sensing of the contents of microfluidic flow channels
WO2003038127A1 (en) 2001-10-30 2003-05-08 Ahram Biosystems Inc. Method and apparatus for amplification of nucleic acid sequences using immobilized dna polymerase
US7691333B2 (en) 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
EP1463796B1 (en) 2001-11-30 2013-01-09 Fluidigm Corporation Microfluidic device and methods of using same
WO2003085379A2 (en) 2002-04-01 2003-10-16 Fluidigm Corporation Microfluidic particle-analysis systems
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
AU2003231912A1 (en) * 2002-06-12 2003-12-31 Tel Aviv Medical Center Research Development Fund Methods of detecting and treating prostate cancer
US7553448B2 (en) * 2002-06-20 2009-06-30 Bioveris Corporation Electrochemiluminescence flow cell and flow cell components
WO2004003219A2 (en) * 2002-06-28 2004-01-08 Igen International, Inc. Improved assay systems and components
JP2005534008A (en) * 2002-07-23 2005-11-10 ザ・テキサス・エイ・アンド・エム・ユニバーシティ・システム Photon signal reporting of electrochemical events
WO2004011145A1 (en) * 2002-07-26 2004-02-05 Arizona Board Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Arizona State University Microreactor with controllable pressure and temperature for in situ material investigations
US20070166725A1 (en) * 2006-01-18 2007-07-19 The Regents Of The University Of California Multiplexed diagnostic platform for point-of care pathogen detection
US20040038385A1 (en) * 2002-08-26 2004-02-26 Langlois Richard G. System for autonomous monitoring of bioagents
US7143785B2 (en) 2002-09-25 2006-12-05 California Institute Of Technology Microfluidic large scale integration
JP5695287B2 (en) 2002-10-02 2015-04-01 カリフォルニア インスティテュート オブ テクノロジー Nucleic acid analysis of microfluids
US20090209030A1 (en) * 2002-10-15 2009-08-20 Benett William J Thermal Cycler
US20040072334A1 (en) * 2002-10-15 2004-04-15 The Regents Of The University Of California Thermal cycler
TW200409821A (en) * 2002-12-03 2004-06-16 Ind Tech Res Inst Microarray biochip reactor
CA2886504C (en) 2002-12-20 2019-01-08 Celera Corporation Wdr12 polymorphisms associated with myocardial infarction, methods of detection and uses thereof
EP1608952B1 (en) 2002-12-20 2016-08-10 Life Technologies Corporation Assay apparatus and method using microfluidic arrays
US10533998B2 (en) 2008-07-18 2020-01-14 Bio-Rad Laboratories, Inc. Enzyme quantification
GB2399776A (en) * 2003-03-24 2004-09-29 Pa Knowledge Ltd Cyclical heating and cooling device and associated methods
US8828663B2 (en) 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
US20050145496A1 (en) 2003-04-03 2005-07-07 Federico Goodsaid Thermal reaction device and method for using the same
AU2004228678A1 (en) 2003-04-03 2004-10-21 Fluidigm Corp. Microfluidic devices and methods of using same
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
US7476363B2 (en) 2003-04-03 2009-01-13 Fluidigm Corporation Microfluidic devices and methods of using same
DE04752947T1 (en) 2003-05-23 2006-11-16 Bio-Rad Laboratories, Inc., Hercules LOCALIZED TEMPERATURE CONTROL FOR ROOM ARRANGEMENTS OF REACTION MEDIA
US7435391B2 (en) * 2003-05-23 2008-10-14 Lucent Technologies Inc. Light-mediated micro-chemical reactors
US7170594B2 (en) 2003-05-28 2007-01-30 Smiths Detection, Inc. Device for polymerase chain reactions
ES2220227B1 (en) 2003-05-30 2006-02-16 INSTITUTO NACIONAL DE TECNICA AEROESPACIAL "ESTEBAN TERRADAS" METHOD AND APPARATUS FOR THE DETECTION OF SUBSTANCES OR ANALYTICS FROM THE ANALYSIS OF ONE OR SEVERAL SAMPLES.
EP2402089A1 (en) 2003-07-31 2012-01-04 Handylab, Inc. Processing particle-containing samples
US7413712B2 (en) 2003-08-11 2008-08-19 California Institute Of Technology Microfluidic rotary flow reactor matrix
US7981362B2 (en) 2003-11-04 2011-07-19 Meso Scale Technologies, Llc Modular assay plates, reader systems and methods for test measurements
CA3050151C (en) 2003-11-26 2023-03-07 Celera Corporation Single nucleotide polymorphisms associated with cardiovascular disorders and statin response, methods of detection and uses thereof
US8697433B2 (en) * 2003-12-10 2014-04-15 Samsung Electronics Co., Ltd. Polymerase chain reaction (PCR) module and multiple PCR system using the same
US7767439B2 (en) * 2003-12-10 2010-08-03 Samsung Electronics Co., Ltd. Real-time PCR monitoring apparatus and method
DE10359160A1 (en) * 2003-12-16 2005-07-21 Roche Diagnostics Gmbh Test element for the examination of sample material
US20050170401A1 (en) * 2004-01-29 2005-08-04 Canon Kabushiki Kaisha Hybridization apparatus and method
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
WO2005108620A2 (en) 2004-05-03 2005-11-17 Handylab, Inc. Processing polynucleotide-containing samples
EP1745154B1 (en) 2004-05-07 2012-08-01 Celera Corporation Genetic polymorphisms associated with liver fibrosis methods of detection and uses thereof
DE102004035080A1 (en) * 2004-05-27 2005-12-29 Infineon Technologies Ag Arrangement for reducing electrical crosstalk on a chip
US7622296B2 (en) 2004-05-28 2009-11-24 Wafergen, Inc. Apparatus and method for multiplex analysis
DE102004033317A1 (en) * 2004-07-09 2006-02-09 Roche Diagnostics Gmbh Analytical test element
WO2006020658A1 (en) * 2004-08-09 2006-02-23 Johnny Yau Method and apparatus for ad hoc mesh routing
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US7391936B2 (en) * 2005-01-21 2008-06-24 Lucent Technologies, Inc. Microfluidic sensors and methods for making the same
WO2006099365A2 (en) 2005-03-11 2006-09-21 Applera Corporation Genetic polymorphisms associated with coronary heart disease, methods of detection and uses thereof
JP2006255522A (en) * 2005-03-15 2006-09-28 Hitachi Ltd Apparatus and method for producing substance
ES2675044T3 (en) 2005-06-03 2018-07-06 Board Of Regents Of The University Of Texas System Electrochemistry and electrogenerated chemiluminescence with a single faradaic electrode
US7780813B2 (en) * 2005-06-09 2010-08-24 Alcatel-Lucent Usa Inc. Electric field mediated chemical reactors
US7630849B2 (en) * 2005-09-01 2009-12-08 Applied Biosystems, Llc Method of automated calibration and diagnosis of laboratory instruments
WO2007028860A1 (en) * 2005-09-06 2007-03-15 Finnzymes Instruments Oy Thermal cycler with optimized sample holder geometry
US7799530B2 (en) 2005-09-23 2010-09-21 Celera Corporation Genetic polymorphisms associated with cardiovascular disorders and drug response, methods of detection and uses thereof
US7754148B2 (en) 2006-12-27 2010-07-13 Progentech Limited Instrument for cassette for sample preparation
US7727473B2 (en) 2005-10-19 2010-06-01 Progentech Limited Cassette for sample preparation
KR100768089B1 (en) * 2005-11-30 2007-10-18 한국전자통신연구원 Affirnity Chromatography microdevice, and preparing method of the same
WO2007081386A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use
WO2008030631A2 (en) 2006-02-03 2008-03-13 Microchip Biotechnologies, Inc. Microfluidic devices
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US8088616B2 (en) 2006-03-24 2012-01-03 Handylab, Inc. Heater unit for microfluidic diagnostic system
JP5415253B2 (en) 2006-03-24 2014-02-12 ハンディラブ・インコーポレーテッド Integrated system for processing microfluidic samples and methods of use thereof
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
EP2016611A2 (en) * 2006-04-25 2009-01-21 University Of South Florida Portable reactor for real-time nucleic acid amplification and detection
US8900828B2 (en) 2006-05-01 2014-12-02 Cepheid Methods and apparatus for sequential amplification reactions
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US20080014589A1 (en) 2006-05-11 2008-01-17 Link Darren R Microfluidic devices and methods of use thereof
US8119352B2 (en) * 2006-06-20 2012-02-21 Cepheld Multi-stage amplification reactions by control of sequence replication times
EP2064345B1 (en) 2006-09-11 2013-03-13 Celera Corporation Genetic polymorphisms associated with psoriasis, methods of detection and uses thereof
KR100758273B1 (en) * 2006-09-13 2007-09-12 한국전자통신연구원 A plastic based microfabricated thermal device and a method for manufacturing the same, and a dna amplification chip and a method for manufacturing the same using the same
CA2915679C (en) 2006-10-20 2017-12-12 Celera Corporation Genetic polymorphisms associated with venous thrombosis, methods of detection and uses thereof
WO2008052168A2 (en) * 2006-10-26 2008-05-02 Symyx Technologies, Inc. High pressure parallel fixed bed reactor and method
WO2008061165A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic cartridge and method of making same
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
WO2008061771A1 (en) * 2006-11-23 2008-05-29 Nanoident Technologies Ag Device for optoelectronically characterizing samples
WO2008091626A1 (en) 2007-01-22 2008-07-31 Wafergen, Inc. Apparatus for high throughput chemical reactions
WO2008115626A2 (en) * 2007-02-05 2008-09-25 Microchip Biotechnologies, Inc. Microfluidic and nanofluidic devices, systems, and applications
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US20080206877A1 (en) * 2007-02-22 2008-08-28 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Reactors for selective enhancement reactions and methods of using such reactors
US8298763B2 (en) * 2007-03-02 2012-10-30 Lawrence Livermore National Security, Llc Automated high-throughput flow-through real-time diagnostic system
DE102007017450A1 (en) * 2007-04-02 2008-10-09 Niro-Plan Ag Method and apparatus for making caffe latte macchiato
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US20100203051A1 (en) 2007-05-31 2010-08-12 The University Of Queensland Diagnostic markers for ankylosing spondylitis and uses thereof
EP2527472B1 (en) 2007-05-31 2016-09-28 Yale University A genetic lesion associated with cancer
US20090136385A1 (en) 2007-07-13 2009-05-28 Handylab, Inc. Reagent Tube
CA2693654C (en) 2007-07-13 2018-02-13 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
USD621060S1 (en) 2008-07-14 2010-08-03 Handylab, Inc. Microfluidic cartridge
US7955840B2 (en) 2007-08-23 2011-06-07 Akonni Biosystems Thermal cycler for PCR including temperature control bladder
WO2009039122A2 (en) 2007-09-17 2009-03-26 Sequenom, Inc. Integrated robotic sample transfer device
US8778663B2 (en) * 2007-09-18 2014-07-15 Lawrence Livermore National Security, Llc. Thermal cycler
US8039212B2 (en) 2007-11-05 2011-10-18 Celera Corporation Genetic polymorphisms associated with liver fibrosis, methods of detection and uses thereof
DE102007062441A1 (en) * 2007-12-20 2009-06-25 Aj Innuscreen Gmbh Mobile rapid test system for nucleic acid analysis
DE102008009920A1 (en) * 2008-02-15 2009-08-20 Aj Innuscreen Gmbh Mobile device for nucleic acid isolation
US20090221620A1 (en) 2008-02-20 2009-09-03 Celera Corporation Gentic polymorphisms associated with stroke, methods of detection and uses thereof
EP2310525B1 (en) 2008-07-09 2014-02-26 Celera Corporation Genetic polymorphisms associated with cardiovascular diseases, methods of detection and uses thereof
USD618820S1 (en) 2008-07-11 2010-06-29 Handylab, Inc. Reagent holder
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
CA2638458A1 (en) * 2008-07-31 2010-01-31 Spartan Bioscience Inc. Thermal recycling by positioning relative to fixed-temperature source
US20100081577A1 (en) * 2008-09-30 2010-04-01 Symyx Technologies, Inc. Reactor systems and methods
WO2010055525A1 (en) 2008-11-17 2010-05-20 Technion Research & Development Foundation Ltd. Method for predicting a patient's responsiveness to anti-folate therapy
US20100261292A1 (en) 2009-04-10 2010-10-14 Meso Scale Technologies, Llc Methods for Conducting Assays
EP3357568A1 (en) 2009-04-14 2018-08-08 Biocartis NV Hifu induced cavitation with reduced power threshold
AU2010237532B2 (en) 2009-04-15 2014-11-20 Biocartis Nv Optical detection system for monitoring rtPCR reaction
JP5758877B2 (en) 2009-04-15 2015-08-05 ビオカルティ ナームローゼ フェノーツハップＢｉｏｃａｒｔｉｓ ＮＶ Bioanalytical sample chamber protection
JP5766180B2 (en) 2009-05-06 2015-08-19 ビオカルティ ナームローゼ フェノーツハップＢｉｏｃａｒｔｉｓ ＮＶ Device for cutting a sample carrier
JP2012529268A (en) 2009-06-05 2012-11-22 インテジェンクス，インコーポレイテッド Use of universal sample preparation system and integrated analysis system
ES2623633T3 (en) 2009-06-09 2017-07-11 Gendiag.Exe, S.L. Risk markers for cardiovascular disease
AU2010265889A1 (en) 2009-06-25 2012-01-19 Yale University Single nucleotide polymorphisms in BRCA1 and cancer risk
EP3611510A1 (en) 2009-07-27 2020-02-19 Meso Scale Technologies, LLC Assay apparatuses, consumables and methods
EP2524027A4 (en) 2010-01-12 2017-10-25 Ahram Biosystems, Inc. Two-stage thermal convection apparatus and uses thereof
CN102791847B (en) 2010-01-12 2015-01-21 阿赫姆生物系统公司 Three-stage thermal convection apparatus and uses thereof
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
WO2011100604A2 (en) 2010-02-12 2011-08-18 Raindance Technologies, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
WO2011106384A1 (en) 2010-02-23 2011-09-01 Genturadx, Inc. Apparatus and methods for integrated sample preparation, reaction and detection
WO2011128096A1 (en) 2010-04-16 2011-10-20 Roche Diagnostics Gmbh Polymorphism markers for predicting response to interleukin-6 receptor-inhibiting monoclonal antibody drug treatment
US20110269735A1 (en) 2010-04-19 2011-11-03 Celera Corporation Genetic polymorphisms associated with statin response and cardiovascular diseases, methods of detection and uses thereof
US9512188B2 (en) 2010-07-12 2016-12-06 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Isolated polynucleotides and methods and plants using same for regulating plant acidity
WO2012024658A2 (en) 2010-08-20 2012-02-23 IntegenX, Inc. Integrated analysis system
WO2012024657A1 (en) 2010-08-20 2012-02-23 IntegenX, Inc. Microfluidic devices with mechanically-sealed diaphragm valves
EP3447155A1 (en) 2010-09-30 2019-02-27 Raindance Technologies, Inc. Sandwich assays in droplets
US20120157332A1 (en) 2010-10-14 2012-06-21 Meso Scale Technologies, Llc Reagent Storage in an Assay Device
US20120108651A1 (en) 2010-11-02 2012-05-03 Leiden University Medical Center (LUMC) Acting on Behalf of Academic Hospital Leiden (AZL) Genetic polymorphisms associated with venous thrombosis and statin response, methods of detection and uses thereof
CA3177188A1 (en) 2011-01-06 2012-07-12 Meso Scale Technologies, Llc. Assay cartridges for pcr analysis and methods of use thereof
WO2012109600A2 (en) 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
CA3082652A1 (en) * 2011-04-15 2012-10-18 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
WO2012151473A2 (en) 2011-05-04 2012-11-08 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
DK2761305T3 (en) 2011-09-30 2017-11-20 Becton Dickinson Co United Reagent Strip
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
EP2773892B1 (en) 2011-11-04 2020-10-07 Handylab, Inc. Polynucleotide sample preparation device
EP2776833B1 (en) 2011-11-11 2018-09-05 Eli N. Glezer Co-binder assisted assay methods
RU2658773C2 (en) 2012-02-03 2018-06-22 Бектон, Дикинсон Энд Компани System and method of implementation of automated assays on plurality of biological samples
EP4345460A2 (en) 2013-01-04 2024-04-03 Meso Scale Technologies, LLC. Assay apparatuses, methods and reagents
WO2014164594A1 (en) 2013-03-11 2014-10-09 Meso Scale Technologies, Llc. Improved methods for conducting multiplexed assays
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
KR102257912B1 (en) 2013-03-13 2021-05-27 메소 스케일 테크놀러지즈, 엘엘시 Improved assay methods
WO2014143623A1 (en) 2013-03-15 2014-09-18 The Lubrizol Corporation Heavy metal free cpvc compositions
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
CN105873681B (en) 2013-11-18 2019-10-11 尹特根埃克斯有限公司 Cartridge and instrument for sample analysis
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
CA2947747C (en) 2014-05-09 2024-03-05 Meso Scale Technologies, Llc. Graphene-modified electrodes
US10408823B2 (en) 2014-05-15 2019-09-10 Meso Scale Technologies, Llc. Assay methods
WO2015179098A1 (en) 2014-05-21 2015-11-26 Integenx Inc. Fluidic cartridge with valve mechanism
US9506908B2 (en) 2014-10-06 2016-11-29 Alveo Technologies, Inc. System for detection of analytes
US10627358B2 (en) 2014-10-06 2020-04-21 Alveo Technologies, Inc. Method for detection of analytes
US9921182B2 (en) 2014-10-06 2018-03-20 ALVEO Technologies Inc. System and method for detection of mercury
US10196678B2 (en) 2014-10-06 2019-02-05 ALVEO Technologies Inc. System and method for detection of nucleic acids
US10352899B2 (en) 2014-10-06 2019-07-16 ALVEO Technologies Inc. System and method for detection of silver
EP3552690A1 (en) 2014-10-22 2019-10-16 IntegenX Inc. Systems and methods for sample preparation, processing and analysis
US9943819B2 (en) 2014-11-03 2018-04-17 Singh Instrument LLC Small-scale reactor having improved mixing
WO2016116935A1 (en) 2015-01-21 2016-07-28 Yeda Research And Development Co. Ltd. Use of rasa2 as a prognostic and therapeutic marker for melanoma
US10641772B2 (en) 2015-02-20 2020-05-05 Takara Bio Usa, Inc. Method for rapid accurate dispensing, visualization and analysis of single cells
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
JP6578188B2 (en) * 2015-11-05 2019-09-18 ウシオ電機株式会社 Optical measuring instrument
EP3387107B1 (en) 2015-12-11 2020-08-12 Spartan Bioscience Inc. Tube sealing system and methods for nucleic acid amplification
EP3472342A1 (en) 2016-06-19 2019-04-24 Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. Screening for chemotherapy resistance in human haploid cells
EP3487616B1 (en) 2016-07-21 2023-08-09 Takara Bio USA, Inc. Multi-z imaging of wells of multi-well devices and liquid dispensing into the wells
CN109996888A (en) 2016-09-23 2019-07-09 阿尔韦奥科技公司 For testing and analyzing the method and composition of object
JP7377544B2 (en) 2017-05-17 2023-11-10 マイクロバイオ・プロプライエタリー・リミテッド Biomarkers and their use
FI3756009T3 (en) 2018-02-23 2023-12-01 Meso Scale Technologies Llc Methods of screening antigen-binding molecules by normalizing for the concentration of antigen-binding molecule
IL262658A (en) 2018-10-28 2020-04-30 Memorial Sloan Kettering Cancer Center Prevention of age related clonal hematopoiesis and diseases associated therewith
CN113366313A (en) 2019-01-03 2021-09-07 中尺度技术有限责任公司 Compositions and methods for performing analytical measurements
DE102019200109A1 (en) * 2019-01-08 2020-07-09 Robert Bosch Gmbh Microfluidic device and analyzer for a microfluidic device
JP2022522480A (en) 2019-03-01 2022-04-19 メソ スケール テクノロジーズ エルエルシー Electrochemiluminescent labeled probe for use in immunoassays, method of using the probe and kit containing the probe
WO2020245377A1 (en) 2019-06-07 2020-12-10 F. Hoffmann-La Roche Ag Hybridizing all-lna oligonucleotides
WO2021236584A1 (en) 2020-05-19 2021-11-25 Meso Scale Technologies, Llc. Methods, compositions, and kits for nucleic acid detection
AU2021299536A1 (en) 2020-07-01 2023-02-16 Meso Scale Technologies, Llc. Compositions and methods for assay measurements
WO2022136234A1 (en) 2020-12-22 2022-06-30 F. Hoffmann-La Roche Ag Method for detecting an analyte of interest in a sample
WO2023129884A1 (en) 2021-12-30 2023-07-06 Meso Scale Technologies, Llc. Methods for electrochemiluminescence detection
WO2023156510A1 (en) 2022-02-18 2023-08-24 F. Hoffmann-La Roche Ag Method for detecting an analyte of interest in a sample
WO2023194369A1 (en) 2022-04-08 2023-10-12 Fundació Institut Mar D'investigacions Mèdiques (Imim) Genetic markers for severe covid-19

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4315890A (en) * 1980-05-01 1982-02-16 Intersci Corporation Device for the identification of volatile fluids
US5252294A (en) * 1988-06-01 1993-10-12 Messerschmitt-Bolkow-Blohm Gmbh Micromechanical structure
US4908112A (en) * 1988-06-16 1990-03-13 E. I. Du Pont De Nemours & Co. Silicon semiconductor wafer for analyzing micronic biological samples
US5262127A (en) * 1992-02-12 1993-11-16 The Regents Of The University Of Michigan Solid state chemical micro-reservoirs
US5304487A (en) * 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
EP0637996B1 (en) * 1992-05-01 1997-07-23 The Trustees Of The University Of Pennsylvania Microfabricated detection structures
US5639423A (en) * 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor

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WO1997000726A1 (en) 1997-01-09
DE69634175D1 (en) 2005-02-17
EP0871545A4 (en) 1999-09-08
JPH11509136A (en) 1999-08-17
US5589136A (en) 1996-12-31
EP0871545A1 (en) 1998-10-21
ES2236739T3 (en) 2005-07-16
CA2225390C (en) 2008-09-30
JP4091656B2 (en) 2008-05-28
EP0871545B1 (en) 2005-01-12

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