CA2227331A1 - Microscale fluid handling system - Google Patents

Microscale fluid handling system

Info

Publication number
CA2227331A1
CA2227331A1 CA002227331A CA2227331A CA2227331A1 CA 2227331 A1 CA2227331 A1 CA 2227331A1 CA 002227331 A CA002227331 A CA 002227331A CA 2227331 A CA2227331 A CA 2227331A CA 2227331 A1 CA2227331 A1 CA 2227331A1
Authority
CA
Canada
Prior art keywords
handling system
chip
fluid handling
sample
conductive
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002227331A
Other languages
French (fr)
Other versions
CA2227331C (en
Inventor
Barry L. Karger
Frantisek Foret
Paul M. Zavracky
E. Nicol Mcgruer
Qifeng Xue
Yuriy M. Dunayevskiy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Northeastern University Boston
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2227331A1 publication Critical patent/CA2227331A1/en
Application granted granted Critical
Publication of CA2227331C publication Critical patent/CA2227331C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/10—Ion sources; Ion guns
    • H01J49/16—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
    • H01J49/165—Electrospray ionisation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0268—Drop counters; Drop formers using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G01N30/724—Nebulising, aerosol formation or ionisation
    • G01N30/7266—Nebulising, aerosol formation or ionisation by electric field, e.g. electrospray
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • H01J49/0431—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components for liquid samples
    • H01J49/0445—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components for liquid samples with means for introducing as a spray, a jet or an aerosol
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00783—Laminate assemblies, i.e. the reactor comprising a stack of plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00819—Materials of construction
    • B01J2219/00822—Metal
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00819—Materials of construction
    • B01J2219/00824—Ceramic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00819—Materials of construction
    • B01J2219/00824—Ceramic
    • B01J2219/00828—Silicon wafers or plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00819—Materials of construction
    • B01J2219/00831—Glass
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00819—Materials of construction
    • B01J2219/00833—Plastic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00851—Additional features
    • B01J2219/00853—Employing electrode arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00873—Heat exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00889—Mixing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00891—Feeding or evacuation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00925—Irradiation
    • B01J2219/0093—Electric or magnetic energy
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00925—Irradiation
    • B01J2219/00932—Sonic or ultrasonic vibrations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/02—Drop detachment mechanisms of single droplets from nozzles or pins
    • B01L2400/027—Drop detachment mechanisms of single droplets from nozzles or pins electrostatic forces between substrate and tip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y80/00—Products made by additive manufacturing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N2030/285—Control of physical parameters of the fluid carrier electrically driven carrier
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N2030/628—Multiplexing, i.e. several columns sharing a single detector
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G01N2035/1044—Using pneumatic means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/466—Flow patterns using more than one column with separation columns in parallel
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6095—Micromachined or nanomachined, e.g. micro- or nanosize
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y10T436/117497—Automated chemical analysis with a continuously flowing sample or carrier stream
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25125—Digestion or removing interfering materials
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

A microscale fluid handling system (10) that permits the efficient transfer of nanoliter to picoliter quantities of a fluid sample from the spatially concentrated environment of a microfabricated chip to "off-chip" analytical or collection devices (23) for further off-chip sample manipu-lation and analysis is disclosed. The fluid handling system (10) is fabricated in the form of one or more channels (12), in any suitable format, provided in a mi-crochip body or substrate of silica, poly-mer or other suitable non-conductive ma-terial, or of stainless steel, noble metal, silicon or other suitable conductive or semi-conductive material. The microchip fluid handling system (10) includes one or more exit ports (16) integral with the end of one or more of the channels (12) for consecutive or simultaneous off chip analysis or collection of the sample. The exit port or ports (16) may be configured, for example, as an electrospray interface for transfer of a fluid sample to a mass spectrometer (23).
CA002227331A 1995-07-21 1996-07-19 Microscale fluid handling system Expired - Lifetime CA2227331C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US134995P 1995-07-21 1995-07-21
US60/001,349 1995-07-21
US08/675,177 US5872010A (en) 1995-07-21 1996-07-03 Microscale fluid handling system
US08/675,177 1996-07-03
PCT/US1996/011985 WO1997004297A1 (en) 1995-07-21 1996-07-19 Microscale fluid handling system

Publications (2)

Publication Number Publication Date
CA2227331A1 true CA2227331A1 (en) 1997-02-06
CA2227331C CA2227331C (en) 2002-04-02

Family

ID=26668907

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002227331A Expired - Lifetime CA2227331C (en) 1995-07-21 1996-07-19 Microscale fluid handling system

Country Status (7)

Country Link
US (1) US5872010A (en)
EP (1) EP0840886B1 (en)
JP (1) JP4489187B2 (en)
AT (1) ATE463732T1 (en)
CA (1) CA2227331C (en)
DE (1) DE69638159D1 (en)
WO (1) WO1997004297A1 (en)

Families Citing this family (422)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US6048734A (en) 1995-09-15 2000-04-11 The Regents Of The University Of Michigan Thermal microvalves in a fluid flow method
US6794127B1 (en) * 1997-06-16 2004-09-21 Diversa Corporation Capillary array-based sample screening
AU2069597A (en) 1996-03-04 1997-09-22 Genetrace Systems, Inc. Methods of screening nucleic acids using mass spectrometry
US5965363A (en) 1996-09-19 1999-10-12 Genetrace Systems Inc. Methods of preparing nucleic acids for mass spectrometric analysis
DE59707302D1 (en) * 1996-09-25 2002-06-20 Sepiatec Gmbh DEVICE AND METHOD ON THE HPLC BASE FOR SEPARATING HIGHLY COMPLEX SUBSTANCE MIXTURES
US6110343A (en) * 1996-10-04 2000-08-29 Lockheed Martin Energy Research Corporation Material transport method and apparatus
DE69727489T2 (en) 1996-11-06 2004-11-25 Sequenom, Inc., San Diego METHOD OF MASS SPECTROMETRY
US6024925A (en) * 1997-01-23 2000-02-15 Sequenom, Inc. Systems and methods for preparing low volume analyte array elements
US7285422B1 (en) 1997-01-23 2007-10-23 Sequenom, Inc. Systems and methods for preparing and analyzing low volume analyte array elements
EP1164203B1 (en) * 1996-11-06 2007-10-10 Sequenom, Inc. DNA Diagnostics based on mass spectrometry
US6379929B1 (en) * 1996-11-20 2002-04-30 The Regents Of The University Of Michigan Chip-based isothermal amplification devices and methods
DE69735445T2 (en) * 1996-12-10 2006-08-10 Sequenom, Inc., San Diego NON-VOLATILE, NON-VOLATILE MOLECULES FOR MASS MARKING
AU6135298A (en) * 1997-01-27 1998-08-26 California Institute Of Technology Mems electrospray nozzle for mass spectroscopy
DE19704477A1 (en) * 1997-02-06 1998-08-13 Solvay Pharm Gmbh Device and method for parallel chromatography
US5980719A (en) * 1997-05-13 1999-11-09 Sarnoff Corporation Electrohydrodynamic receptor
WO1998052034A1 (en) * 1997-05-15 1998-11-19 Bio-Rad Laboratories, Inc. Thermal expansion pump for pulse-free liquid flow
WO1998052691A1 (en) * 1997-05-16 1998-11-26 Alberta Research Council Microfluidic system and methods of use
US6632619B1 (en) 1997-05-16 2003-10-14 The Governors Of The University Of Alberta Microfluidic system and methods of use
US5989402A (en) 1997-08-29 1999-11-23 Caliper Technologies Corp. Controller/detector interfaces for microfluidic systems
CA2299439C (en) * 1997-09-12 2007-08-14 Bruce A. Andrien Multiple sample introduction mass spectrometry
WO1999014368A2 (en) * 1997-09-15 1999-03-25 Whitehead Institute For Biomedical Research Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device
WO1999015888A1 (en) * 1997-09-19 1999-04-01 Aclara Biosciences, Inc. Capillary electroflow apparatus and method
US6838051B2 (en) 1999-05-03 2005-01-04 Ljl Biosystems, Inc. Integrated sample-processing system
US6902703B2 (en) * 1999-05-03 2005-06-07 Ljl Biosystems, Inc. Integrated sample-processing system
US6982431B2 (en) * 1998-08-31 2006-01-03 Molecular Devices Corporation Sample analysis systems
US6833242B2 (en) * 1997-09-23 2004-12-21 California Institute Of Technology Methods for detecting and sorting polynucleotides based on size
WO1999019899A1 (en) * 1997-10-15 1999-04-22 Analytica Of Branford, Inc. Curved introduction for mass spectrometry
US6139734A (en) * 1997-10-20 2000-10-31 University Of Virginia Patent Foundation Apparatus for structural characterization of biological moieties through HPLC separation
US6685809B1 (en) * 1999-02-04 2004-02-03 Ut-Battelle, Llc Methods for forming small-volume electrical contacts and material manipulations with fluidic microchannels
GB9802600D0 (en) * 1998-02-07 1998-04-01 Eastman Kodak Co Liquid separation
US6379971B1 (en) 1998-02-24 2002-04-30 Target Discovery, Inc. Methods for sequencing proteins
US6537432B1 (en) 1998-02-24 2003-03-25 Target Discovery, Inc. Protein separation via multidimensional electrophoresis
US6191418B1 (en) * 1998-03-27 2001-02-20 Synsorb Biotech, Inc. Device for delivery of multiple liquid sample streams to a mass spectrometer
AU4546899A (en) * 1998-06-05 1999-12-20 Sarnoff Corporation Apparatus for separating molecules
US6459080B1 (en) * 1998-06-12 2002-10-01 Agilent Technologies, Inc. Miniaturized device for separating the constituents of a sample and delivering the constituents of the separated sample to a mass spectrometer
US6627446B1 (en) * 1998-07-02 2003-09-30 Amersham Biosciences (Sv) Corp Robotic microchannel bioanalytical instrument
US20030138973A1 (en) * 1998-07-14 2003-07-24 Peter Wagner Microdevices for screening biomolecules
US6576478B1 (en) * 1998-07-14 2003-06-10 Zyomyx, Inc. Microdevices for high-throughput screening of biomolecules
US6794197B1 (en) 1998-07-14 2004-09-21 Zyomyx, Inc. Microdevice and method for detecting a characteristic of a fluid
GB9815820D0 (en) * 1998-07-22 1998-09-16 Secr Defence Improvements relating to micro-machining
US6090278A (en) * 1998-08-20 2000-07-18 Dyax Corporation Apparatus and method for sealing a plurality of chromatography columns
US6074880A (en) * 1998-08-28 2000-06-13 Transgenomic, Inc. Sample analyte containing solution fraction collection system, and method of use
US6410332B1 (en) * 1998-09-08 2002-06-25 Symyx Technologies, Inc. Sampling and analysis of reactions by trapping reaction components on a sorbent
US6103199A (en) * 1998-09-15 2000-08-15 Aclara Biosciences, Inc. Capillary electroflow apparatus and method
US6245227B1 (en) 1998-09-17 2001-06-12 Kionix, Inc. Integrated monolithic microfabricated electrospray and liquid chromatography system and method
CN1312474C (en) * 1998-09-17 2007-04-25 阿德文生物科学公司 Integrated chemical analysis system
WO2000019193A1 (en) * 1998-09-28 2000-04-06 Varian Inc Split flow electrospray device for mass spectrometry
DE19846466A1 (en) * 1998-10-08 2000-04-27 Ghs Gesundheits Service Ag Analysis method for the simultaneous determination of parameters from different media
US6605472B1 (en) 1998-10-09 2003-08-12 The Governors Of The University Of Alberta Microfluidic devices connected to glass capillaries with minimal dead volume
US6234167B1 (en) 1998-10-14 2001-05-22 Chrysalis Technologies, Incorporated Aerosol generator and methods of making and using an aerosol generator
US6680193B1 (en) 1998-10-16 2004-01-20 Commissariat A L'energie Atomique Device for chemical and/or biological analysis with analysis support
WO2000030167A1 (en) * 1998-11-19 2000-05-25 California Institute Of Technology Polymer-based electrospray nozzle for mass spectrometry
NL1010833C2 (en) * 1998-12-17 2000-06-20 Univ Delft Tech Method for the dosed application of a liquid to a surface.
CA2357623A1 (en) * 1999-01-08 2000-07-13 Frantisek Foret Electro-pneumatic distributor for multiplexed .mu.-tas devices
EP1157270A1 (en) * 1999-02-03 2001-11-28 Aclara BioSciences, Inc. Multichannel control in microfluidics
GB9904038D0 (en) * 1999-02-22 1999-04-14 Isis Innovation Improvements in or related to microfluidic sample preparation and mass spectrometry
US6633031B1 (en) * 1999-03-02 2003-10-14 Advion Biosciences, Inc. Integrated monolithic microfabricated dispensing nozzle and liquid chromatography-electrospray system and method
US7150994B2 (en) * 1999-03-03 2006-12-19 Symyx Technologies, Inc. Parallel flow process optimization reactor
US6749814B1 (en) 1999-03-03 2004-06-15 Symyx Technologies, Inc. Chemical processing microsystems comprising parallel flow microreactors and methods for using same
US6410914B1 (en) 1999-03-05 2002-06-25 Bruker Daltonics Inc. Ionization chamber for atmospheric pressure ionization mass spectrometry
DE19913076A1 (en) * 1999-03-23 2000-10-19 Hahn Schickard Ges Device and method for applying microdroplets to a substrate
US7247490B2 (en) * 1999-04-06 2007-07-24 Uab Research Foundation Method for screening crystallization conditions in solution crystal growth
US7214540B2 (en) * 1999-04-06 2007-05-08 Uab Research Foundation Method for screening crystallization conditions in solution crystal growth
US7250305B2 (en) * 2001-07-30 2007-07-31 Uab Research Foundation Use of dye to distinguish salt and protein crystals under microcrystallization conditions
US20030022383A1 (en) * 1999-04-06 2003-01-30 Uab Research Foundation Method for screening crystallization conditions in solution crystal growth
ATE357656T1 (en) * 1999-04-06 2007-04-15 Univ Alabama Res Found DEVICE FOR SCREENING CRYSTALIZATION CONDITIONS IN CRYSTAL GROWING SOLUTIONS
US7244396B2 (en) * 1999-04-06 2007-07-17 Uab Research Foundation Method for preparation of microarrays for screening of crystal growth conditions
WO2000062039A1 (en) * 1999-04-09 2000-10-19 Northeastern University System and method for high throughput mass spectrometric analysis
US6368562B1 (en) * 1999-04-16 2002-04-09 Orchid Biosciences, Inc. Liquid transportation system for microfluidic device
US6677114B1 (en) * 1999-04-20 2004-01-13 Target Discovery, Inc. Polypeptide fingerprinting methods and bioinformatics database system
US6716636B1 (en) 1999-04-20 2004-04-06 Target Discovery, Inc. Methods for sequencing proteins
US6764817B1 (en) * 1999-04-20 2004-07-20 Target Discovery, Inc. Methods for conducting metabolic analyses
US20030119069A1 (en) * 1999-04-20 2003-06-26 Target Discovery, Inc. Labeling of protein samples
IL146093A0 (en) * 1999-04-20 2002-07-25 Target Discovery Inc Method of separating a polypeptide or protein from a solution containing a plurality thereof
US6818112B2 (en) * 1999-04-20 2004-11-16 Target Discovery, Inc. Protein separation via multidimensional electrophoresis
US6649358B1 (en) 1999-06-01 2003-11-18 Caliper Technologies Corp. Microscale assays and microfluidic devices for transporter, gradient induced, and binding activities
US6630006B2 (en) * 1999-06-18 2003-10-07 The Regents Of The University Of California Method for screening microcrystallizations for crystal formation
US6296673B1 (en) 1999-06-18 2001-10-02 The Regents Of The University Of California Methods and apparatus for performing array microcrystallizations
DE19928412C2 (en) * 1999-06-22 2002-03-21 Agilent Technologies Inc Supply element for a laboratory microchip
US6533914B1 (en) * 1999-07-08 2003-03-18 Shaorong Liu Microfabricated injector and capillary array assembly for high-resolution and high throughput separation
US6448090B1 (en) 1999-07-09 2002-09-10 Orchid Biosciences, Inc. Fluid delivery system for a microfluidic device using alternating pressure waveforms
US6395232B1 (en) * 1999-07-09 2002-05-28 Orchid Biosciences, Inc. Fluid delivery system for a microfluidic device using a pressure pulse
DE19933458B4 (en) * 1999-07-15 2015-08-20 Eppendorf Ag Equipment and systems for handling liquid samples
US6660147B1 (en) * 1999-07-16 2003-12-09 Applera Corporation High density electrophoresis device and method
US7015030B1 (en) 1999-07-28 2006-03-21 Genset S.A. Microfluidic devices and uses thereof in biochemical processes
DE19935433A1 (en) * 1999-08-01 2001-03-01 Febit Ferrarius Biotech Gmbh Microfluidic reaction carrier
JP3925000B2 (en) * 1999-09-06 2007-06-06 株式会社日立製作所 Nebulizer and analyzer using the same
DE19947495C2 (en) 1999-10-01 2003-05-28 Agilent Technologies Inc Microfluidic microchip
DE19947496C2 (en) 1999-10-01 2003-05-22 Agilent Technologies Inc Microfluidic microchip
US6478238B1 (en) 1999-11-03 2002-11-12 Cornell Research Foundation Inc. Miniaturized fluid transfer device
DE29919506U1 (en) * 1999-11-05 2000-02-24 Creavis Tech & Innovation Gmbh Microstructured pipettes as dosing systems
US6878255B1 (en) * 1999-11-05 2005-04-12 Arrowhead Center, Inc. Microfluidic devices with thick-film electrochemical detection
US7167615B1 (en) 1999-11-05 2007-01-23 Board Of Regents, The University Of Texas System Resonant waveguide-grating filters and sensors and methods for making and using same
CA2290731A1 (en) * 1999-11-26 2001-05-26 D. Jed Harrison Apparatus and method for trapping bead based reagents within microfluidic analysis system
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
WO2001050499A1 (en) 1999-12-30 2001-07-12 Advion Biosciences, Inc. Multiple electrospray device, systems and methods
WO2001051918A1 (en) * 2000-01-12 2001-07-19 Ut-Battelle, Llc A microfluidic device and method for focusing, segmenting, and dispensing of a fluid stream
CA2397415A1 (en) * 2000-01-12 2001-07-19 Zyomyx, Inc. Microdevice and method for detecting a characteristic of a fluid
WO2001053794A1 (en) * 2000-01-18 2001-07-26 Northeastern University Parallel sample loading and injection device for multichannel microfluidic devices
US6939452B2 (en) * 2000-01-18 2005-09-06 Northeastern University Parallel sample loading and injection device for multichannel microfluidic devices
US6596988B2 (en) * 2000-01-18 2003-07-22 Advion Biosciences, Inc. Separation media, multiple electrospray nozzle system and method
US6967324B2 (en) * 2000-02-17 2005-11-22 Agilent Technologies, Inc. Micro matrix ion generator for analyzers
US6720157B2 (en) * 2000-02-23 2004-04-13 Zyomyx, Inc. Chips having elevated sample surfaces
WO2001064344A2 (en) 2000-03-02 2001-09-07 Microchips, Inc. Microfabricated devices for the storage and selective exposure of chemicals and devices
WO2001066245A2 (en) 2000-03-07 2001-09-13 Symyx Technologies, Inc. Parallel flow process optimization reactor
FR2806077B1 (en) * 2000-03-07 2004-01-30 Solvay PROCESS FOR OBTAINING A PURE HYDROFLUOROALKANE, PURE HYDROFLUOROALKANE, USE OF THE HYDROFLUOROALKANE AND METHOD OF ANALYSIS OF A HYDROFLUOROALKANE
US7077960B2 (en) * 2000-03-07 2006-07-18 Solvay (Societe Anonyme) Method for obtaining a purified hydrofluoroalkane, purified hydrofluoroalkane, use of same and method for analysing same
US6569383B1 (en) * 2000-03-11 2003-05-27 Intrinsic Bioprobes, Inc. Bioactive chip mass spectrometry
FR2806646A1 (en) * 2000-03-22 2001-09-28 Francois Geli MULTIBLOCK MICRO-ARRAYS OR MACRO-ARRAYS WITH LABORATORIES ON INTEGRATED CHIPS
WO2001077640A2 (en) * 2000-04-05 2001-10-18 Alexion Pharmaceuticals, Inc. Methods and devices for storing and dispensing liquids
US6561208B1 (en) 2000-04-14 2003-05-13 Nanostream, Inc. Fluidic impedances in microfluidic system
MY136453A (en) * 2000-04-27 2008-10-31 Philip Morris Usa Inc "improved method and apparatus for generating an aerosol"
WO2001091902A2 (en) 2000-05-30 2001-12-06 Massachusetts Institute Of Technology Methods and devices for sealing microchip reservoir devices
US20020027197A1 (en) * 2000-06-05 2002-03-07 Duholke Wayne K. Multiple source electrospray ionization for mass spectrometry
ATE423221T1 (en) * 2000-06-13 2009-03-15 Univ Boston USE OF MASS-MATCHED NUCLEOTIDES IN THE ANALYSIS OF OLIGONUCLEOTIDE MIXTURES AND IN HIGH-MULTIPLEX NUCLEIC ACID SEQUENCING
US6829753B2 (en) * 2000-06-27 2004-12-07 Fluidigm Corporation Microfluidic design automation method and system
US6544477B1 (en) 2000-08-01 2003-04-08 Regents Of The University Of Minnesota Apparatus for generating a temperature gradient
US6701774B2 (en) * 2000-08-02 2004-03-09 Symyx Technologies, Inc. Parallel gas chromatograph with microdetector array
AU2001281076A1 (en) * 2000-08-07 2002-02-18 Nanostream, Inc. Fluidic mixer in microfluidic system
US6890093B2 (en) 2000-08-07 2005-05-10 Nanostream, Inc. Multi-stream microfludic mixers
GB2366793B (en) * 2000-09-13 2005-03-09 Imperial College Chemical processing system and method
US6613224B1 (en) 2000-10-06 2003-09-02 Waters Investments Limited Liquid separation column smart cartridge
US6827095B2 (en) * 2000-10-12 2004-12-07 Nanostream, Inc. Modular microfluidic systems
AU1189702A (en) * 2000-10-13 2002-04-22 Fluidigm Corp Microfluidic device based sample injection system for analytical devices
EP2479578A1 (en) * 2000-10-19 2012-07-25 Target Discovery, Inc. Mass defect labeling for the determination of oligomer sequences
US7033475B2 (en) * 2000-10-25 2006-04-25 Shimadzu Corporation Electrophoretic apparatus
US20020142483A1 (en) 2000-10-30 2002-10-03 Sequenom, Inc. Method and apparatus for delivery of submicroliter volumes onto a substrate
US7514046B2 (en) * 2000-10-31 2009-04-07 Caliper Life Sciences, Inc. Methods and systems for processing microscale devices for reuse
US6879162B2 (en) * 2000-11-07 2005-04-12 Sri International System and method of micro-fluidic handling and dispensing using micro-nozzle structures
DE10154601B4 (en) * 2000-11-13 2007-02-22 Agilent Technologies, Inc. (n.d.Ges.d.Staates Delaware), Palo Alto A micro device with an integrated protruding electrospray emitter and a method of fabricating the micro device
AU2002248149A1 (en) * 2000-11-16 2002-08-12 Fluidigm Corporation Microfluidic devices for introducing and dispensing fluids from microfluidic systems
US6841774B1 (en) 2000-11-28 2005-01-11 Mds Inc. Sample introduction device for mass spectrometry using a fast fluidic system to synchronize multiple parallel liquid sample streams
DE60144243D1 (en) * 2000-11-28 2011-04-28 Dh Technologies Dev Pte Ltd DEVICE FOR INTRODUCING LIQUID SAMPLES INTO A MASS SPECTROMETER USING A QUICK VALVE SYSTEM FOR SYNCHRONIZING MULTIPLE PARALLEL SAMPLES
SE0004574D0 (en) * 2000-12-08 2000-12-08 Amersham Pharm Biotech Ab Electrospray interface
SE0004594D0 (en) * 2000-12-12 2000-12-12 Gyros Ab Microscale nozzie
US6491233B2 (en) 2000-12-22 2002-12-10 Chrysalis Technologies Incorporated Vapor driven aerosol generator and method of use thereof
US6701921B2 (en) 2000-12-22 2004-03-09 Chrysalis Technologies Incorporated Aerosol generator having heater in multilayered composite and method of use thereof
US6501052B2 (en) 2000-12-22 2002-12-31 Chrysalis Technologies Incorporated Aerosol generator having multiple heating zones and methods of use thereof
US7077130B2 (en) * 2000-12-22 2006-07-18 Chrysalis Technologies Incorporated Disposable inhaler system
US6799572B2 (en) 2000-12-22 2004-10-05 Chrysalis Technologies Incorporated Disposable aerosol generator system and methods for administering the aerosol
US6681998B2 (en) 2000-12-22 2004-01-27 Chrysalis Technologies Incorporated Aerosol generator having inductive heater and method of use thereof
US20040099310A1 (en) * 2001-01-05 2004-05-27 Per Andersson Microfluidic device
US6653625B2 (en) * 2001-03-19 2003-11-25 Gyros Ab Microfluidic system (MS)
CN1253238C (en) * 2001-01-26 2006-04-26 阿德维昂生物科学公司 Robotic autosampler for automated electrospray from microfluidic chip
GB0103516D0 (en) * 2001-02-13 2001-03-28 Cole Polytechnique Federale De Apparatus for dispensing a sample
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
WO2002066135A1 (en) * 2001-02-20 2002-08-29 Advion Biosciences, Inc. A microchip electrospray device and column with affinity adsorbents and use of the same
US7118917B2 (en) * 2001-03-07 2006-10-10 Symyx Technologies, Inc. Parallel flow reactor having improved thermal control
EP1384076B1 (en) 2001-03-19 2012-07-25 Gyros Patent Ab Characterization of reaction variables
JP2004529463A (en) * 2001-03-19 2004-09-24 ユィロス・アクチボラグ Micro fluid system (MS)
CA2442342A1 (en) * 2001-03-19 2002-09-26 Gyros Ab A microfluidic system (edi)
EP1384249A1 (en) * 2001-03-19 2004-01-28 Gyros AB A microfluidic system (ms)
US6717136B2 (en) 2001-03-19 2004-04-06 Gyros Ab Microfludic system (EDI)
US6610978B2 (en) * 2001-03-27 2003-08-26 Agilent Technologies, Inc. Integrated sample preparation, separation and introduction microdevice for inductively coupled plasma mass spectrometry
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US7192557B2 (en) * 2001-03-28 2007-03-20 Handylab, Inc. Methods and systems for releasing intracellular material from cells within microfluidic samples of fluids
US7323140B2 (en) * 2001-03-28 2008-01-29 Handylab, Inc. Moving microdroplets in a microfluidic device
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US7270786B2 (en) 2001-03-28 2007-09-18 Handylab, Inc. Methods and systems for processing microfluidic samples of particle containing fluids
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US6575188B2 (en) 2001-07-26 2003-06-10 Handylab, Inc. Methods and systems for fluid control in microfluidic devices
US7670429B2 (en) * 2001-04-05 2010-03-02 The California Institute Of Technology High throughput screening of crystallization of materials
AU2002307152A1 (en) * 2001-04-06 2002-10-21 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
WO2002090770A2 (en) * 2001-05-07 2002-11-14 Nanolab Ltd. Method and apparatus for propelling a fluid
US20020166760A1 (en) * 2001-05-11 2002-11-14 Prentiss Mara G. Micromagentic systems and methods for microfluidics
AU2002326304A1 (en) * 2001-05-31 2002-12-16 Massachusetts Institute Of Technology Microchip devices with improved reservoir opening
US20020187557A1 (en) * 2001-06-07 2002-12-12 Hobbs Steven E. Systems and methods for introducing samples into microfluidic devices
US6919046B2 (en) * 2001-06-07 2005-07-19 Nanostream, Inc. Microfluidic analytical devices and methods
US6811695B2 (en) * 2001-06-07 2004-11-02 Nanostream, Inc. Microfluidic filter
EP1399135B1 (en) * 2001-06-28 2004-12-29 Microchips, Inc. Methods for hermetically sealing microchip reservoir devices
GB0116384D0 (en) * 2001-07-04 2001-08-29 Diagnoswiss Sa Microfluidic chemical assay apparatus and method
US7128876B2 (en) * 2001-07-17 2006-10-31 Agilent Technologies, Inc. Microdevice and method for component separation in a fluid
US7060227B2 (en) * 2001-08-06 2006-06-13 Sau Lan Tang Staats Microfluidic devices with raised walls
US6995024B2 (en) * 2001-08-27 2006-02-07 Sri International Method and apparatus for electrostatic dispensing of microdroplets
US6803568B2 (en) * 2001-09-19 2004-10-12 Predicant Biosciences, Inc. Multi-channel microfluidic chip for electrospray ionization
US6568390B2 (en) 2001-09-21 2003-05-27 Chrysalis Technologies Incorporated Dual capillary fluid vaporizing device
US6640050B2 (en) 2001-09-21 2003-10-28 Chrysalis Technologies Incorporated Fluid vaporizing device having controlled temperature profile heater/capillary tube
US20030072682A1 (en) * 2001-10-11 2003-04-17 Dan Kikinis Method and apparatus for performing biochemical testing in a microenvironment
US8440093B1 (en) 2001-10-26 2013-05-14 Fuidigm Corporation Methods and devices for electronic and magnetic sensing of the contents of microfluidic flow channels
US6681769B2 (en) 2001-12-06 2004-01-27 Crysalis Technologies Incorporated Aerosol generator having a multiple path heater arrangement and method of use thereof
US6804458B2 (en) * 2001-12-06 2004-10-12 Chrysalis Technologies Incorporated Aerosol generator having heater arranged to vaporize fluid in fluid passage between bonded layers of laminate
US20030141253A1 (en) * 2001-12-07 2003-07-31 Bihan Thierry Le Apparatus for efficient liquid chromatography/mass spectrometry processing
US6701922B2 (en) 2001-12-20 2004-03-09 Chrysalis Technologies Incorporated Mouthpiece entrainment airflow control for aerosol generators
US7105810B2 (en) * 2001-12-21 2006-09-12 Cornell Research Foundation, Inc. Electrospray emitter for microfluidic channel
US6877892B2 (en) * 2002-01-11 2005-04-12 Nanostream, Inc. Multi-stream microfluidic aperture mixers
WO2003066684A2 (en) * 2002-01-18 2003-08-14 The Regents Of The University Of Michigan Porous polymers: compositions and uses thereof
US7189370B2 (en) * 2002-02-11 2007-03-13 Microchem Solutions Apparatus and methods for high throughput and high-resolution assays
DE60303122T9 (en) * 2002-02-13 2007-02-22 Nanostream, Inc., Pasadena MICROFLUIDIC SEPARATION COLLONS AND THEIR MANUFACTURING PROCESS
US7261812B1 (en) 2002-02-13 2007-08-28 Nanostream, Inc. Multi-column separation devices and methods
US7033542B2 (en) * 2002-02-14 2006-04-25 Archibald William B High throughput screening with parallel vibrational spectroscopy
US20050211555A1 (en) * 2002-02-14 2005-09-29 Solus Biosystems, Inc. Method for multiple sample screening using IR spectroscopy
US6958119B2 (en) * 2002-02-26 2005-10-25 Agilent Technologies, Inc. Mobile phase gradient generation microfluidic device
WO2003076052A1 (en) * 2002-03-05 2003-09-18 Caliper Life Sciences, Inc. Mixed mode microfluidic systems
US7303727B1 (en) 2002-03-06 2007-12-04 Caliper Life Sciences, Inc Microfluidic sample delivery devices, systems, and methods
AU2003285849A1 (en) * 2002-03-20 2004-03-29 Advanced Sensor Technologies, Inc. Personal monitor to detect exposure to toxic agents
EP1499706A4 (en) 2002-04-01 2010-11-03 Fluidigm Corp Microfluidic particle-analysis systems
US7312085B2 (en) * 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
US7901939B2 (en) * 2002-05-09 2011-03-08 University Of Chicago Method for performing crystallization and reactions in pressure-driven fluid plugs
ATE479899T1 (en) * 2002-05-09 2010-09-15 Univ Chicago EQUIPMENT AND METHODS FOR PRESSURE CONTROLLED PLUG TRANSPORT AND REACTION
US20030224531A1 (en) * 2002-05-29 2003-12-04 Brennen Reid A. Microplate with an integrated microfluidic system for parallel processing minute volumes of fluids
US20070026528A1 (en) * 2002-05-30 2007-02-01 Delucas Lawrence J Method for screening crystallization conditions in solution crystal growth
AU2003247442A1 (en) * 2002-06-05 2003-12-22 Advanced Research And Technology Institute, Inc. Apparatus and method for relative or quantitative comparison of multiple samples
WO2003104792A2 (en) * 2002-06-07 2003-12-18 Picosep A/S A method of separating biocomponents contained in a liquid, a separating system and a separating unit
US20060086309A1 (en) * 2002-06-24 2006-04-27 Fluiding Corporation Recirculating fluidic network and methods for using the same
US20040007672A1 (en) * 2002-07-10 2004-01-15 Delucas Lawrence J. Method for distinguishing between biomolecule and non-biomolecule crystals
US20040018115A1 (en) * 2002-07-29 2004-01-29 Nanostream, Inc. Fault tolerant detection regions in microfluidic systems
JP3610349B2 (en) * 2002-08-06 2005-01-12 キヤノン株式会社 Liquid transfer device
WO2004015411A1 (en) * 2002-08-08 2004-02-19 Nanostream, Inc. Systems and methods for high-throughput microfluidic sample analysis
US7214320B1 (en) 2002-08-08 2007-05-08 Nanostream, Inc. Systems and methods for high throughput sample analysis
US7132650B1 (en) 2003-09-26 2006-11-07 Nanostream, Inc. High throughput multi-dimensional sample analysis
EP1551753A2 (en) 2002-09-25 2005-07-13 California Institute Of Technology Microfluidic large scale integration
JP5695287B2 (en) 2002-10-02 2015-04-01 カリフォルニア インスティテュート オブ テクノロジー Nucleic acid analysis of microfluids
US7173240B2 (en) 2004-11-05 2007-02-06 Agilent Technologies, Inc. Electrospray devices for mass spectrometry
US7946982B2 (en) 2002-10-25 2011-05-24 K2M, Inc. Minimal incision maximal access MIS spine instrumentation and method
US6936167B2 (en) * 2002-10-31 2005-08-30 Nanostream, Inc. System and method for performing multiple parallel chromatographic separations
GB0226160D0 (en) * 2002-11-08 2002-12-18 Diagnoswiss Sa Apparatus for dispensing a sample in electrospray mass spectrometers
JP2006507504A (en) * 2002-11-22 2006-03-02 ソルス バイオシステムズ インコーポレーテッド High-throughput screening by parallel vibrational spectroscopy
JP2004354364A (en) 2002-12-02 2004-12-16 Nec Corp Fine particle manipulating unit, chip mounted with the same and detector, and method for separating, capturing and detecting protein
US20060076482A1 (en) * 2002-12-13 2006-04-13 Hobbs Steven E High throughput systems and methods for parallel sample analysis
US6987263B2 (en) * 2002-12-13 2006-01-17 Nanostream, Inc. High throughput systems and methods for parallel sample analysis
US20040129676A1 (en) * 2003-01-07 2004-07-08 Tan Roy H. Apparatus for transfer of an array of liquids and methods for manufacturing same
DE10302721A1 (en) * 2003-01-23 2004-08-05 Steag Microparts Gmbh Microfluidic arrangement for dosing liquids
US7413709B2 (en) * 2003-02-12 2008-08-19 Agilent Technologies, Inc. PAEK-based microfluidic device with integrated electrospray emitter
SE0300454D0 (en) * 2003-02-19 2003-02-19 Aamic Ab Nozzles for electrospray ionization and methods of fabricating them
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
GB0307428D0 (en) * 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
US7007710B2 (en) * 2003-04-21 2006-03-07 Predicant Biosciences, Inc. Microfluidic devices and methods
US7074327B2 (en) * 2003-05-08 2006-07-11 Nanostream, Inc. Sample preparation for parallel chromatography
US6945285B2 (en) * 2003-05-14 2005-09-20 Industrial Technology Research Institute Apparatus and method for collecting fluid fractions
US7425700B2 (en) * 2003-05-22 2008-09-16 Stults John T Systems and methods for discovery and analysis of markers
US20040236603A1 (en) * 2003-05-22 2004-11-25 Biospect, Inc. System of analyzing complex mixtures of biological and other fluids to identify biological state information
US20040265519A1 (en) * 2003-06-27 2004-12-30 Pellizzari Roberto O. Fabrication of fluid delivery components
US7731906B2 (en) 2003-07-31 2010-06-08 Handylab, Inc. Processing particle-containing samples
JP4217739B2 (en) * 2003-08-04 2009-02-04 カリパー・ライフ・サイエンシズ・インク． Method and system for processing microscale devices for reuse
US7028536B2 (en) * 2004-06-29 2006-04-18 Nanostream, Inc. Sealing interface for microfluidic device
US20050032238A1 (en) * 2003-08-07 2005-02-10 Nanostream, Inc. Vented microfluidic separation devices and methods
US7413712B2 (en) * 2003-08-11 2008-08-19 California Institute Of Technology Microfluidic rotary flow reactor matrix
US7053558B2 (en) * 2003-09-03 2006-05-30 Sri International System and method for controlling emission by a micro-fabricated charge-emission device
FR2860006B1 (en) * 2003-09-24 2006-12-22 Commissariat Energie Atomique DEVICE FOR SEPARATING AND / OR ANALYZING MULTIPLE MOLECULAR TARGETS IN SOLUTION IN A COMPLEX MIXTURE
US7537807B2 (en) * 2003-09-26 2009-05-26 Cornell University Scanned source oriented nanofiber formation
US7282705B2 (en) * 2003-12-19 2007-10-16 Agilent Technologies, Inc. Microdevice having an annular lining for producing an electrospray emitter
WO2005073732A2 (en) * 2004-01-23 2005-08-11 Amgen Inc. Lc/ms method of analyzing high molecular weight proteins
FR2865806B1 (en) * 2004-01-30 2007-02-02 Commissariat Energie Atomique ON-CHIP LABORATORY COMPRISING A MICRO-FLUIDIC NETWORK AND A COPLANAR ELECTRONEBULATING NOSE
CN100340856C (en) * 2004-02-03 2007-10-03 复旦大学 Nano-liter spraying device
EP1715954A1 (en) * 2004-02-18 2006-11-02 Applera Corporation Multi-step bioassays on modular microfluidic application platforms
US20050221339A1 (en) * 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US20050244973A1 (en) * 2004-04-29 2005-11-03 Predicant Biosciences, Inc. Biological patterns for diagnosis and treatment of cancer
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
AU2005241080B2 (en) * 2004-05-03 2011-08-11 Handylab, Inc. Processing polynucleotide-containing samples
KR100777172B1 (en) * 2004-06-07 2007-11-28 나노 퓨전 가부시키가이샤 Electroosmotic pump system and electroosmotic pump
WO2005121307A2 (en) * 2004-06-07 2005-12-22 Bioprocessor Corp. Gas control in a microreactor
US9477233B2 (en) * 2004-07-02 2016-10-25 The University Of Chicago Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets
KR100573926B1 (en) 2004-07-02 2006-04-26 한국과학기술원 Continuous multi-cell sorting device using 3D optical potential in micro channel
US7655470B2 (en) * 2004-10-29 2010-02-02 University Of Chicago Method for manipulating a plurality of plugs and performing reactions therein in microfluidic systems
US20060022130A1 (en) * 2004-07-29 2006-02-02 Predicant Biosciences, Inc., A Delaware Corporation Microfluidic devices and methods with integrated electrical contact
EP1794581A2 (en) 2004-09-15 2007-06-13 Microchip Biotechnologies, Inc. Microfluidic devices
US20060060769A1 (en) * 2004-09-21 2006-03-23 Predicant Biosciences, Inc. Electrospray apparatus with an integrated electrode
US7591883B2 (en) * 2004-09-27 2009-09-22 Cornell Research Foundation, Inc. Microfiber supported nanofiber membrane
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
KR20060058830A (en) * 2004-11-26 2006-06-01 한국표준과학연구원 A separation method for multi channel electrophoresis device having no individual sample wells
US20080074646A1 (en) * 2005-01-18 2008-03-27 Solus Biosystems, Inc. Multiple Sample Screening Using Ir Spectroscopy with Capillary Isoelectric Focusing
CN101107507B (en) * 2005-01-25 2012-10-24 欧西里其有限责任公司 Temperature controller for small fluid samples having different heat capacities
EP1623763A1 (en) * 2005-03-11 2006-02-08 Agilent Technologies, Inc. Chip with cleaning cavity
JP2008538077A (en) * 2005-03-16 2008-10-09 ユニバーシティ オブ シカゴ Micro fluidic system
US20090136362A1 (en) * 2005-03-30 2009-05-28 Nano Fusion Technologies Inc. Electroosmosis Pump and Liquid Feeding Device
JP2006275016A (en) * 2005-03-30 2006-10-12 Science Solutions International Laboratory Inc Liquid transport device and liquid transport system
GB0511083D0 (en) * 2005-05-31 2005-07-06 Thermo Finnigan Llc Multiple ion injection in mass spectrometry
KR20070074787A (en) * 2005-06-13 2007-07-18 삼성전자주식회사 Gray voltage generator and liquid crystal display apparatus
FR2887241B1 (en) * 2005-06-15 2008-01-11 Rhodia Chimie Sa MICROFLUIDIC FLOW DEVICE FOR DETERMINING PARAMETERS OF PHYSICAL AND / OR CHEMICAL TRANSFORMATION AND USE THEREOF
EP1890803B1 (en) 2005-06-15 2010-07-21 Rhodia Opérations Microfluidic circulating device for determining parameters of a physical and/or chemical transformation, and use thereof
US20070023677A1 (en) * 2005-06-29 2007-02-01 Perkins Patrick D Multimode ionization source and method for screening molecules
US20070158192A1 (en) * 2005-07-29 2007-07-12 West Virginia University Research Corporation Apparatus and method for coupling microfluidic systems with a mass spectrometer utilizing rapid voltage switching
WO2007044917A2 (en) * 2005-10-11 2007-04-19 Handylab, Inc. Polynucleotide sample preparation device
EP1953542B9 (en) * 2005-11-08 2013-11-13 Tohoku University Method of quantifying membrane protein by using mass spectrometer
US20070140918A1 (en) * 2005-12-19 2007-06-21 Hongfeng Yin Fluidic separation devices and methods with reduced sample broadening
EP2363205A3 (en) 2006-01-11 2014-06-04 Raindance Technologies, Inc. Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors
WO2007089777A2 (en) * 2006-01-31 2007-08-09 University Of Chicago Method and apparatus for assaying blood clotting
JP5063616B2 (en) * 2006-02-03 2012-10-31 インテジェニックス インコーポレイテッド Microfluidic device
FR2897858B1 (en) * 2006-02-27 2008-06-20 Commissariat Energie Atomique METHOD FOR MANUFACTURING A NETWORK OF CAPILLARIES OF A CHIP
US7998708B2 (en) * 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
JP5415253B2 (en) 2006-03-24 2014-02-12 ハンディラブ・インコーポレーテッド Integrated system for processing microfluidic samples and methods of use thereof
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US8088616B2 (en) 2006-03-24 2012-01-03 Handylab, Inc. Heater unit for microfluidic diagnostic system
US20070221839A1 (en) * 2006-03-24 2007-09-27 Goodley Paul C Analytical system and method
US7495210B2 (en) 2006-05-04 2009-02-24 Agilent Technologies, Inc. Micro fluidic gas assisted ionization device and method
EP2530168B1 (en) 2006-05-11 2015-09-16 Raindance Technologies, Inc. Microfluidic Devices
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US7629124B2 (en) * 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
WO2008021123A1 (en) 2006-08-07 2008-02-21 President And Fellows Of Harvard College Fluorocarbon emulsion stabilizing surfactants
US9892650B2 (en) * 2006-09-11 2018-02-13 Houghton Mifflin Harcourt Publishing Company Recovery of polled data after an online test platform failure
WO2008061165A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic cartridge and method of making same
US20100078077A1 (en) * 2006-12-19 2010-04-01 Ismagilov Rustem F Spacers for Microfluidic Channels
CN101715483A (en) 2007-02-05 2010-05-26 微芯片生物工艺学股份有限公司 microfluidic and nanofluidic devices, systems, and applications
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
KR100786622B1 (en) 2007-02-16 2007-12-21 한국표준과학연구원 Multi Channel Electrophoresis Device Having No Individual and Sample Wells and gene or protein seperate method using thereof
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
CN101680013B (en) 2007-06-11 2014-12-03 和光纯药工业株式会社 Microchip large-volume PCR with integrated real-time CE detection
USD621060S1 (en) 2008-07-14 2010-08-03 Handylab, Inc. Microfluidic cartridge
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8105783B2 (en) * 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US20090136385A1 (en) * 2007-07-13 2009-05-28 Handylab, Inc. Reagent Tube
WO2009012185A1 (en) 2007-07-13 2009-01-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US20100227767A1 (en) * 2007-07-26 2010-09-09 Boedicker James Q Stochastic confinement to detect, manipulate, and utilize molecules and organisms
US20090180931A1 (en) 2007-09-17 2009-07-16 Sequenom, Inc. Integrated robotic sample transfer device
US7825337B2 (en) * 2007-10-23 2010-11-02 Slam Brands, Inc. Cable management apparatuses and systems
US8381169B2 (en) * 2007-10-30 2013-02-19 International Business Machines Corporation Extending unified process and method content to include dynamic and collaborative content
WO2009108260A2 (en) 2008-01-22 2009-09-03 Microchip Biotechnologies, Inc. Universal sample preparation system and use in an integrated analysis system
US8622987B2 (en) * 2008-06-04 2014-01-07 The University Of Chicago Chemistrode, a plug-based microfluidic device and method for stimulation and sampling with high temporal, spatial, and chemical resolution
GB0812781D0 (en) * 2008-07-11 2008-08-20 Deltadot Ltd Material separation device
USD618820S1 (en) 2008-07-11 2010-06-29 Handylab, Inc. Reagent holder
US20100009351A1 (en) * 2008-07-11 2010-01-14 Handylab, Inc. Polynucleotide Capture Materials, and Method of Using Same
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
US8534307B2 (en) * 2008-08-30 2013-09-17 Corning Incorporated Methods and devices for fluid handling
US8672532B2 (en) * 2008-12-31 2014-03-18 Integenx Inc. Microfluidic methods
WO2010102199A1 (en) 2009-03-06 2010-09-10 Waters Technologies Corporation Electromechanical and fluidic interface to a microfluidic substrate
EP3415235A1 (en) 2009-03-23 2018-12-19 Raindance Technologies Inc. Manipulation of microfluidic droplets
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
AU2010229490B2 (en) 2009-03-24 2015-02-12 University Of Chicago Slip chip device and methods
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
CN102459565A (en) * 2009-06-02 2012-05-16 尹特根埃克斯有限公司 Fluidic devices with diaphragm valves
AU2010257118B2 (en) * 2009-06-04 2014-08-28 Lockheed Martin Corporation Multiple-sample microfluidic chip for DNA analysis
JP2012529268A (en) * 2009-06-05 2012-11-22 インテジェンクス，インコーポレイテッド Use of universal sample preparation system and integrated analysis system
WO2011042564A1 (en) 2009-10-09 2011-04-14 Universite De Strasbourg Labelled silica-based nanomaterial with enhanced properties and uses thereof
US8584703B2 (en) * 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
EP2517025B1 (en) 2009-12-23 2019-11-27 Bio-Rad Laboratories, Inc. Methods for reducing the exchange of molecules between droplets
US20110165037A1 (en) * 2010-01-07 2011-07-07 Ismagilov Rustem F Interfaces that eliminate non-specific adsorption, and introduce specific interactions
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
EP2534267B1 (en) 2010-02-12 2018-04-11 Raindance Technologies, Inc. Digital analyte analysis
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
US8763642B2 (en) 2010-08-20 2014-07-01 Integenx Inc. Microfluidic devices with mechanically-sealed diaphragm valves
US9121058B2 (en) 2010-08-20 2015-09-01 Integenx Inc. Linear valve arrays
US9562897B2 (en) 2010-09-30 2017-02-07 Raindance Technologies, Inc. Sandwich assays in droplets
GB2497501A (en) 2010-10-15 2013-06-12 Lockheed Corp Micro fluidic optic design
TWI532530B (en) * 2010-10-29 2016-05-11 萬國商業機器公司 Multilayer microfluidic probe head with immersion channels and fabrication thereof
US8963080B2 (en) * 2011-01-22 2015-02-24 Michael Charles Nyman Substrate compositions and methods of use thereof
US9364803B2 (en) 2011-02-11 2016-06-14 Raindance Technologies, Inc. Methods for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
EP2684207B1 (en) * 2011-03-11 2018-07-11 The University of North Carolina At Chapel Hill Microchips with integrated multiple electrospray ionization emitters and related methods, systems and devices
US9396916B2 (en) 2011-03-11 2016-07-19 Waters Technologies Corporation Electrokinetically controlled calibrant delivery
CN106190806B (en) 2011-04-15 2018-11-06 贝克顿·迪金森公司 Scan real-time microfluid thermal cycler and the method for synchronous thermal cycle and scanning optical detection
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
EP3709018A1 (en) 2011-06-02 2020-09-16 Bio-Rad Laboratories, Inc. Microfluidic apparatus for identifying components of a chemical reaction
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US11053535B2 (en) 2011-09-12 2021-07-06 The University Of North Carolina At Chapel Hill Devices with a fluid transport nanochannel intersected by a fluid sensing nanochannel and related methods
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
KR102121853B1 (en) 2011-09-30 2020-06-12 벡톤 디킨슨 앤드 컴퍼니 Unitized reagent strip
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
CN104040238B (en) 2011-11-04 2017-06-27 汉迪拉布公司 Polynucleotides sample preparation apparatus
CN107881219B (en) 2012-02-03 2021-09-10 贝克顿·迪金森公司 External file for molecular diagnostic test assignment and compatibility determination between tests
WO2013119765A1 (en) 2012-02-10 2013-08-15 The University Of North Carolina At Chapel Hill Devices with fluidic nanofunnels, associated methods, fabrication and analysis systems
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2881685C (en) 2012-08-14 2023-12-05 10X Genomics, Inc. Microcapsule compositions and methods
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2954065B1 (en) 2013-02-08 2021-07-28 10X Genomics, Inc. Partitioning and processing of analytes and other species
CA2902903A1 (en) 2013-02-28 2014-09-04 The University Of North Carolina At Chapel Hill Nanofluidic devices with integrated components for the controlled capture, trapping, and transport of macromolecules and related methods of analysis
NZ711033A (en) 2013-03-13 2020-03-27 Univ North Carolina Chapel Hill Nanofluidic devices for the rapid mapping of whole genomes and related systems and methods of analysis
US9653276B2 (en) * 2013-08-07 2017-05-16 DH Technologies Development Pte Ltd. Enhanced spray formation for liquid samples
WO2015051343A1 (en) * 2013-10-03 2015-04-09 Northwestern University High throughput mass spectrometric analysis of proteome samples
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
WO2015073999A1 (en) 2013-11-18 2015-05-21 Integenx Inc. Cartridges and instruments for sample analysis
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
WO2015092494A1 (en) * 2013-12-20 2015-06-25 Agilent Technologies, Inc. Method of conducting electrospray ionization and method of analyzing an analyte utilizing the same
US10076751B2 (en) 2013-12-30 2018-09-18 General Electric Company Systems and methods for reagent storage
US9399216B2 (en) 2013-12-30 2016-07-26 General Electric Company Fluid transport in microfluidic applications with sensors for detecting fluid presence and pressure
US11193176B2 (en) 2013-12-31 2021-12-07 Bio-Rad Laboratories, Inc. Method for detecting and quantifying latent retroviral RNA species
AU2015243445B2 (en) 2014-04-10 2020-05-28 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
GB2544198B (en) 2014-05-21 2021-01-13 Integenx Inc Fluidic cartridge with valve mechanism
CN106795553B (en) 2014-06-26 2021-06-04 10X基因组学有限公司 Methods of analyzing nucleic acids from individual cells or cell populations
WO2015196303A1 (en) * 2014-06-27 2015-12-30 Uvic Industry Partnerships Inc. System and method for matrix-coating samples for mass spectrometry
CN107106983B (en) 2014-10-22 2021-04-16 尹特根埃克斯有限公司 Systems and methods for sample preparation, processing, and analysis
KR20170073667A (en) 2014-10-29 2017-06-28 10엑스 제노믹스, 인크. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
CN112126675B (en) 2015-01-12 2022-09-09 10X基因组学有限公司 Method and system for preparing nucleic acid sequencing library and library prepared by using same
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
EP3262188B1 (en) 2015-02-24 2021-05-05 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
US9255905B1 (en) 2015-05-11 2016-02-09 The University Of North Carolina At Chapel Hill Pressure driven microfluidic injection for chemical separations
US10734216B2 (en) 2015-05-11 2020-08-04 The University Of North Carolina At Chapel Hill Pressure driven fluidic injection for chemical separations by electrophoresis
EP3295148A4 (en) 2015-05-11 2018-11-07 The University of North Carolina at Chapel Hill Fluidic devices with nanoscale manifolds for molecular transport, related systems and methods of analysis
US9606082B2 (en) 2015-05-11 2017-03-28 The University Of North Carolina At Chapel Hill Pressure driven microfluidic injection for chemical separations
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
US9728387B2 (en) 2015-10-20 2017-08-08 The University Of North Carolina At Chapel Hill Solid phase extraction with capillary electrophoresis
US10774370B2 (en) 2015-12-04 2020-09-15 10X Genomics, Inc. Methods and compositions for nucleic acid analysis
US9733232B1 (en) * 2016-01-25 2017-08-15 International Business Machines Corporation Nanopillar arrays with interfaces for controlled polymer stretching and effective translocation into nanochannels
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
WO2018003046A1 (en) * 2016-06-30 2018-01-04 株式会社島津製作所 Flow controller
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
EP4230746A3 (en) 2017-05-26 2023-11-01 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
JP6891953B2 (en) 2017-05-31 2021-06-18 株式会社島津製作所 Sample plate for PESI ion source and mass spectrometer using the sample plate
CA3082352A1 (en) * 2017-11-10 2019-05-16 Perkinelmer Health Sciences Canada, Inc. Multiple analyte ion source
JP7038209B2 (en) 2017-11-13 2022-03-17 エフ．ホフマン－ラ ロシュ アーゲー Equipment for sample analysis using epitaco electrophoresis
EP3954782A1 (en) 2017-11-15 2022-02-16 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CN112262218A (en) 2018-04-06 2021-01-22 10X基因组学有限公司 System and method for quality control in single cell processing
CN112090603B (en) * 2019-06-17 2022-11-08 苏州天健云康信息科技有限公司 Microfluidic device and method for manufacturing the same
WO2021026172A1 (en) 2019-08-05 2021-02-11 Seer, Inc. Systems and methods for sample preparation, data generation, and protein corona analysis

Family Cites Families (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3538744A (en) * 1967-11-09 1970-11-10 Phillips Petroleum Co Chromatography apparatus
DE1919628C3 (en) * 1969-04-18 1975-04-10 Wolfgang Prof. Dr. Dittrich Arrangement for the automatic counting and / or classification of particles dispersed in a flowable medium
US3915652A (en) * 1973-08-16 1975-10-28 Samuel Natelson Means for transferring a liquid in a capillary open at both ends to an analyzing system
DE2521236C3 (en) * 1975-05-10 1978-12-14 Hildegard Dr. 4400 Muenster Goehde Geb. Kuhl Device for counting and measuring particles suspended in a liquid
US4366118A (en) * 1978-06-14 1982-12-28 Bunce Roger A Apparatus and method for luminescent determination of concentration of an analyte in a sample
CA1129498A (en) * 1978-10-25 1982-08-10 Richard L. Columbus Structural configuration and method for transport of a liquid drop through an ingress aperture
DE3070217D1 (en) * 1979-10-31 1985-03-28 Univ Birmingham Improvements in or relating to pipette means
DE3068716D1 (en) * 1979-11-08 1984-08-30 Secr Social Service Brit Apparatus for testing a liquid sample
US4593728A (en) * 1982-11-20 1986-06-10 Whitehead Thomas P Dispensing device and recording apparatus
GB2191110B (en) * 1986-06-06 1989-12-06 Plessey Co Plc Chromatographic separation device
US4708782A (en) * 1986-09-15 1987-11-24 Sepragen Corporation Chromatography column-electrophoresis system
EP0286088B1 (en) * 1987-04-08 1994-09-14 Hitachi, Ltd. A sheath flow type flow-cell device
US4908112A (en) * 1988-06-16 1990-03-13 E. I. Du Pont De Nemours & Co. Silicon semiconductor wafer for analyzing micronic biological samples
US5132012A (en) * 1988-06-24 1992-07-21 Hitachi, Ltd. Liquid chromatograph
US5110745A (en) * 1989-06-01 1992-05-05 The Trustees Of The University Of Pennsylvania Methods of detecting glycated proteins
JP2977895B2 (en) * 1989-10-17 1999-11-15 ブリティッシュ・テクノロジー・グループ・リミテッド Amplified chemiluminescent assay
US5302533A (en) * 1989-10-17 1994-04-12 British Technology Group Limited Antibody-enhanced chemiluminescence reactions
US5126022A (en) * 1990-02-28 1992-06-30 Soane Tecnologies, Inc. Method and device for moving molecules by the application of a plurality of electrical fields
SE470347B (en) * 1990-05-10 1994-01-31 Pharmacia Lkb Biotech Microstructure for fluid flow systems and process for manufacturing such a system
HUT60934A (en) * 1990-08-28 1992-11-30 Emil Mincsovics Process, apparatus and sorbent layer for thin-layer chromatography
US5269900A (en) * 1990-09-13 1993-12-14 University Of North Carolina At Chapel Hill Method and device for high speed separation of complex molecules
DE59108006D1 (en) * 1991-01-28 1996-08-22 Ciba Geigy Ag Device for the preparation of samples, in particular for analysis purposes
US5332481A (en) * 1991-01-29 1994-07-26 Beckman Instruments, Inc. Capillary electrophoresis using replaceable gels
DE4104075C1 (en) * 1991-02-11 1992-03-19 Bruker Analytische Messtechnik Gmbh, 7512 Rheinstetten, De
DE4133885C2 (en) * 1991-10-12 1996-03-21 Bosch Gmbh Robert Three-dimensional silicon structure
US5245185A (en) * 1991-11-05 1993-09-14 Georgia Tech Research Corporation Interface device and process to couple planar electrophoresis with spectroscopic methods of detection
EP0544969B1 (en) * 1991-12-06 1997-03-05 Ciba-Geigy Ag Apparatus and method for electrophoretic separation
US5486335A (en) * 1992-05-01 1996-01-23 Trustees Of The University Of Pennsylvania Analysis based on flow restriction
JP3558294B2 (en) * 1992-05-01 2004-08-25 トラスティーズ・オブ・ザ・ユニバーシティ・オブ・ペンシルベニア Polynucleotide amplification analysis using microfabrication equipment
US5744366A (en) * 1992-05-01 1998-04-28 Trustees Of The University Of Pennsylvania Mesoscale devices and methods for analysis of motile cells
US5296375A (en) * 1992-05-01 1994-03-22 Trustees Of The University Of Pennsylvania Mesoscale sperm handling devices
US5498392A (en) * 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US5304487A (en) * 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
US5338427A (en) * 1993-02-26 1994-08-16 Biometric Imaging Inc. Single use separation cartridge for a capillary electrophoresis instrument
US5512451A (en) * 1993-04-01 1996-04-30 British Technology Group Limited Enhancement of chemiluminescent reactions
US5387329A (en) * 1993-04-09 1995-02-07 Ciba Corning Diagnostics Corp. Extended use planar sensors
CA2097257A1 (en) * 1993-05-28 1994-11-29 Norman J. Dovichi Continuous biochemical reactor for analysis of sub-picomole quantities of complex organic molecules and method of operation thereof
DE4318407A1 (en) * 1993-06-03 1994-12-08 Rossendorf Forschzent Microcapillary with integrated chemical microsensors and process for their manufacture
US5328578A (en) * 1993-06-15 1994-07-12 Hewlett-Packard Company Capillary electrophoresis with tracking separation field
US5349186A (en) * 1993-06-25 1994-09-20 The Governors Of The University Of Alberta Electrospray interface for mass spectrometer and method of supplying analyte to a mass spectrometer
US5512131A (en) * 1993-10-04 1996-04-30 President And Fellows Of Harvard College Formation of microstamped patterns on surfaces and derivative articles
US5374834A (en) * 1993-10-12 1994-12-20 Massachusetts Institute Of Technology Ionic liquid-channel charge-coupled device
US5429734A (en) * 1993-10-12 1995-07-04 Massachusetts Institute Of Technology Monolithic capillary electrophoretic device
US5401963A (en) * 1993-11-01 1995-03-28 Rosemount Analytical Inc. Micromachined mass spectrometer
US6001229A (en) * 1994-08-01 1999-12-14 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis
US5658413A (en) * 1994-10-19 1997-08-19 Hewlett-Packard Company Miniaturized planar columns in novel support media for liquid phase analysis
US5500071A (en) * 1994-10-19 1996-03-19 Hewlett-Packard Company Miniaturized planar columns in novel support media for liquid phase analysis
ES2169770T3 (en) * 1994-11-14 2002-07-16 Univ Pennsylvania MESOSCALAR DEVICES FOR POLINUCLEOTID AMPLIFICATION.
ATE269160T1 (en) * 1994-11-14 2004-07-15 Univ Pennsylvania MINIATURIZED SAMPLE PREPARATION DEVICES AND SYSTEMS FOR DETECTING AND TREATING ANALYTES

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JP4489187B2 (en) 2010-06-23
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EP0840886A1 (en) 1998-05-13
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EP0840886B1 (en) 2010-04-07
EP0840886A4 (en) 2005-12-21
JP2002515820A (en) 2002-05-28
ATE463732T1 (en) 2010-04-15
CA2227331C (en) 2002-04-02

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