CA2244326A1 - Microparticle enhanced light scattering agglutination assay and microparticle reagents therefor - Google Patents

Microparticle enhanced light scattering agglutination assay and microparticle reagents therefor

Info

Publication number
CA2244326A1
CA2244326A1 CA002244326A CA2244326A CA2244326A1 CA 2244326 A1 CA2244326 A1 CA 2244326A1 CA 002244326 A CA002244326 A CA 002244326A CA 2244326 A CA2244326 A CA 2244326A CA 2244326 A1 CA2244326 A1 CA 2244326A1
Authority
CA
Canada
Prior art keywords
light scattering
microparticle
analyte
assay
enhanced light
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002244326A
Other languages
French (fr)
Other versions
CA2244326C (en
Inventor
Shinichi Eda
Jorg Heinrich Kaufmann
Stefan Pohl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CA2244326A1 publication Critical patent/CA2244326A1/en
Application granted granted Critical
Publication of CA2244326C publication Critical patent/CA2244326C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding

Abstract

The invention relates to a new microparticle enhanced light scattering agglutination assay for determining the amount of an analyte, and a microparticle reagent for performing that assay. The microparticle enhanced light scattering agglutination tests are quasihomogeneous and do not need a separation and washing step. They thus meet the requirements for the automation with commonly used clinical chemistry analyzers or dedicated nephelometers. Such tests provide an increased sensitivity by a factor of 2 to 3 orders of magnitude (down to 10 -11 mol/l analyte) compared to direct agglutination tests not using microparticles and, in addition, less matrix interference and higher flexibility.
The specification provides a microparticle enhanced light scattering agglutination assay for determining the amount of an analyte which comprises using a mixture of particles of strong light scattering properties carrying at least one binding partner of high reactivity for the analyte and particles of weak light scattering properties carrying at least one binding partner of low reactivity for the analyte. The assay of the invention shows an unexpectedly high dynamic range (DR).
CA002244326A 1997-08-11 1998-07-29 Microparticle enhanced light scattering agglutination assay and microparticle reagents therefor Expired - Lifetime CA2244326C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97113873 1997-08-11
EP97113873.0 1997-08-11

Publications (2)

Publication Number Publication Date
CA2244326A1 true CA2244326A1 (en) 1999-02-11
CA2244326C CA2244326C (en) 2006-03-28

Family

ID=8227200

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002244326A Expired - Lifetime CA2244326C (en) 1997-08-11 1998-07-29 Microparticle enhanced light scattering agglutination assay and microparticle reagents therefor

Country Status (6)

Country Link
US (2) US6248597B1 (en)
JP (1) JP3746902B2 (en)
AT (1) ATE213065T1 (en)
CA (1) CA2244326C (en)
DE (1) DE69803729T2 (en)
ES (1) ES2170984T3 (en)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001274571B2 (en) * 2000-06-20 2007-02-08 Mitsubishi Chemical Corporation Micro-array of organism-associated substance
US6764825B1 (en) * 2000-10-13 2004-07-20 Tang J. Wang Methods and device for detecting prostate specific antigen (PSA)
AU2002231008A1 (en) * 2000-12-19 2002-07-01 Instrumentation Laboratory Co. Free analyte detection system
EP1385001A4 (en) * 2001-03-30 2007-07-25 Mitsubishi Kagaku Iatron Inc Reagent and method for immunoanalysis of elastase 1 and method of detecting pancreatic disease
JP4577747B2 (en) * 2001-06-05 2010-11-10 和光純薬工業株式会社 Aggregation accelerator for immunoassay
US7790439B2 (en) * 2001-08-09 2010-09-07 Panasonic Corporation Biosensor and measurement method
US6551788B1 (en) * 2001-11-28 2003-04-22 Beckman Coulter, Inc. Particle-based ligand assay with extended dynamic range
US8257967B2 (en) * 2002-04-26 2012-09-04 Board Of Regents, The University Of Texas System Method and system for the detection of cardiac risk factors
AU2004225599B2 (en) * 2003-03-31 2008-09-04 Denka Company Limited Turbidimetric immunoassy for lipoprotein (a) and reagent therefor
US20040197841A1 (en) * 2003-04-02 2004-10-07 Apffel James Alexander Methods and reagents for multiplexed analyses
DE602004003632T2 (en) * 2003-07-09 2007-10-25 Matsushita Electric Industrial Co., Ltd., Kadoma Turbidimetric immunoassay method and associated apparatus
US20050095726A1 (en) * 2003-07-14 2005-05-05 Xiangming Fang Novel high protein tortillas
AU2004274866B2 (en) * 2003-08-01 2007-12-13 Ge Healthcare Bio-Science Ab Method and apparatus for quantitating surface-binding optical resonance profiles
JP4241301B2 (en) * 2003-09-30 2009-03-18 和光純薬工業株式会社 Reagent for immunological measurement
AU2004300168A1 (en) 2003-12-11 2005-06-30 Board Of Regents, The University Of Texas System Method and system for the analysis of saliva using a sensor array
US8101431B2 (en) 2004-02-27 2012-01-24 Board Of Regents, The University Of Texas System Integration of fluids and reagents into self-contained cartridges containing sensor elements and reagent delivery systems
US8105849B2 (en) 2004-02-27 2012-01-31 Board Of Regents, The University Of Texas System Integration of fluids and reagents into self-contained cartridges containing sensor elements
DE102004023402A1 (en) 2004-05-12 2005-12-08 Roche Diagnostics Gmbh Method for increasing the dynamic measuring range of, in particular immunological test elements based on specific binding reactions
JP4331073B2 (en) 2004-08-31 2009-09-16 デンカ生研株式会社 Antigen measurement method and reagent therefor
US7638291B2 (en) * 2004-10-25 2009-12-29 Seradyn, Inc. Immunoassays for topiramate
US20060115865A1 (en) * 2004-10-25 2006-06-01 Anlong Ouyang Lamotrigine analogs
US20060088886A1 (en) * 2004-10-25 2006-04-27 Anlong Ouyang Topiramate analogs
US7678551B2 (en) * 2004-10-25 2010-03-16 Seradyn, Inc. Immunoassays for lamotrigine
GB0426822D0 (en) * 2004-12-07 2005-01-12 Precisense As Sensor for detection of glucose
US20060246522A1 (en) * 2005-04-28 2006-11-02 Bhullar Balwant S C-reactive protein immunoassay and method
AU2006309284B2 (en) 2005-05-31 2012-08-02 Board Of Regents, The University Of Texas System Methods and compositions related to determination and use of white blood cell counts
WO2007002480A2 (en) * 2005-06-24 2007-01-04 Board Of Regents, The University Of Texas System Systems and methods including self-contained cartridges with detection systems and fluid delivery systems
WO2007024825A2 (en) * 2005-08-26 2007-03-01 Genway Biotech, Inc. Immunoaffinity separation and analysis compositions and methods
ES2346572T3 (en) * 2005-11-24 2010-10-18 Gentian As TURBIDIMETRIC IMMUNO TEST TO ASSESS HUMAN CYSTERINE C.
EP2147312B1 (en) * 2007-05-21 2012-08-15 Gentian AS Turbidimetric immunoassay for assessing human cystatin c
JP2009085703A (en) * 2007-09-28 2009-04-23 Fujifilm Corp Method of high-sensitivity immunoassay
WO2010058860A1 (en) * 2008-11-18 2010-05-27 株式会社シノテスト Measurement method and measurement reagent for c-reactive protein in sample
EP2357475B1 (en) * 2008-12-04 2016-05-04 Sekisui Medical Co., Ltd. Method for measuring cystatin c in human body fluid
EP2241886A1 (en) * 2009-04-15 2010-10-20 Beckman Coulter Biomedical Limited Homogeneous agglutination immunoassay method and kit for such method
WO2011034678A1 (en) * 2009-09-21 2011-03-24 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona Use of superhydrophobic surfaces for liquid agglutination assays
KR20120107497A (en) * 2009-12-17 2012-10-02 아박시스, 인크. Novel assays for detecting analytes in samples and kits and compositions related thereto
CN101833010B (en) * 2010-03-29 2013-02-13 上海太阳生物技术有限公司 Kit for detecting fibrin (ogen) degradation products (FDP) (by using emulsion immunoturbidimetry)
JP5972871B2 (en) 2010-07-20 2016-08-17 テバ・ファーマシューティカルズ・オーストラリア・ピーティワイ・リミテッド Anti-IL-23 heterodimer specific antibody
WO2012142656A1 (en) 2011-04-18 2012-10-26 Garvan Institute Of Medical Research Method of diagnosing cancer
WO2012161226A1 (en) * 2011-05-23 2012-11-29 積水メディカル株式会社 Method for inhibiting non-specific reaction in pivka-ii measurement reagent
JP5772425B2 (en) 2011-09-13 2015-09-02 ソニー株式会社 Fine particle measuring device
CN104114577A (en) 2011-09-30 2014-10-22 特瓦制药澳大利亚私人有限公司 Antibodies against TL1a and uses thereof
CA2804843C (en) 2012-02-06 2021-02-02 Ortho-Clinical Diagnostics, Inc. Multiple time windows for extending the range of an assay
CN102608325B (en) * 2012-02-24 2016-08-24 南京诺尔曼生物技术有限公司 Cardic fatty acid binding protein (H-FABP) measures test kit (latex enhancing immune turbidimetry)
WO2014113717A1 (en) 2013-01-18 2014-07-24 Ark Diagnostics, Inc. Voriconazole immunoassays
US9840559B2 (en) 2013-02-01 2017-12-12 The Regents Of The University Of California Anti-CD83 antibodies and use thereof
CN110551217B (en) 2013-02-07 2023-12-15 Csl有限公司 IL-11R binding proteins and uses thereof
WO2014126861A1 (en) 2013-02-13 2014-08-21 Ark Diagnostics, Inc. Posaconazole immunoassays
FR3003353B1 (en) * 2013-03-14 2015-03-06 Horiba Abx Sas NOVEL METHOD FOR FLOWING AN OBJECT OF INTEREST
US20160195522A1 (en) 2013-05-31 2016-07-07 Sekisui Medical Co., Ltd. Method of agglutination immunoassay
EP2811300A1 (en) * 2013-06-07 2014-12-10 Roche Diagniostics GmbH Calibration for multi-component assays
EP3194624B1 (en) 2014-09-15 2022-02-16 Garvan Institute of Medical Research Methods for diagnosis, prognosis and monitoring of breast cancer and reagents therefor
NZ731491A (en) 2014-10-23 2021-12-24 Kira Biotech Pty Ltd Cd83 binding proteins and uses thereof
EP3265074B1 (en) 2015-03-03 2020-08-12 ARK Diagnostics, Inc. Pregabalin immunoassays
KR102582602B1 (en) 2015-05-05 2023-09-25 메조블라스트 인터내셔널 에스에이알엘 efficacy test
EP4137562A1 (en) 2017-05-04 2023-02-22 Mesoblast International Sàrl Mesenchymal lineage precursor or stem cells with enhanced immunosuppression
CN109633167A (en) * 2018-12-20 2019-04-16 北京贝尔生物工程股份有限公司 A kind of human serum amyloid A assay kit of highly sensitive, wide detection range
JP2022515916A (en) 2019-01-02 2022-02-22 メゾブラスト・インターナショナル・エスアーエールエル How to treat back pain
US11578140B2 (en) 2019-03-26 2023-02-14 Microgenics Corporation Immunoassay for mitragynine
US10745492B1 (en) 2019-04-03 2020-08-18 Ark Diagnostics, Inc. Antibodies to symmetrically dimethylated arginine analytes and use thereof
CN113125699B (en) * 2019-12-31 2024-03-26 科美博阳诊断技术(上海)有限公司 beta-hCG homogeneous phase chemiluminescence detection kit and application thereof
KR20220159382A (en) 2020-03-30 2022-12-02 덴카 주식회사 Method for measuring target substance by latex agglutination method and reagent therefor
CN116057173A (en) 2020-08-10 2023-05-02 迈索布拉斯特国际有限公司 Composition comprising mesenchymal precursor cells or stem cells and uses thereof
WO2024009226A1 (en) 2022-07-05 2024-01-11 Mesoblast International Sarl Cryopreserved intermediate and potency assay for same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4210723A (en) 1976-07-23 1980-07-01 The Dow Chemical Company Method of coupling a protein to an epoxylated latex
US4401765A (en) * 1981-09-01 1983-08-30 E. I. Du Pont De Nemours And Company Covalently bonded high refractive index particle reagents and their use in light scattering immunoassays
DE3366740D1 (en) * 1982-08-06 1986-11-13 Int Inst Cellular Molecul Path Particle agglutination assay of antigens
US4595661A (en) 1983-11-18 1986-06-17 Beckman Instruments, Inc. Immunoassays and kits for use therein which include low affinity antibodies for reducing the hook effect
US4590169A (en) 1983-11-18 1986-05-20 Beckman Instruments, Inc. Direct particle agglutination immunoassays avoiding false negatives at high antigen concentrations
NO164622C (en) 1988-05-11 1990-10-24 Tore Lindmo BINAER IMMUNOMETRIC PARTICLE-BASED METHOD FOR MEASURING SPECIFIC SERUM ANTIGENS USING LIQUID FLOW MICROPHOTOMETRY AND A PREPARED TARGET SET UP THEREOF.
US5200315A (en) * 1990-07-25 1993-04-06 Eastman Kodak Company Particulate biologically active reagent containing polyoxyalkylene side chains, analytical element and methods for use of the reagent

Also Published As

Publication number Publication date
DE69803729T2 (en) 2002-09-05
ATE213065T1 (en) 2002-02-15
US6828158B2 (en) 2004-12-07
CA2244326C (en) 2006-03-28
ES2170984T3 (en) 2002-08-16
US6248597B1 (en) 2001-06-19
JPH11108929A (en) 1999-04-23
JP3746902B2 (en) 2006-02-22
DE69803729D1 (en) 2002-03-21
US20010026945A1 (en) 2001-10-04

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