CA2251755A1 - Photolabile polymer array synthesis methods - Google Patents
Photolabile polymer array synthesis methodsInfo
- Publication number
- CA2251755A1 CA2251755A1 CA002251755A CA2251755A CA2251755A1 CA 2251755 A1 CA2251755 A1 CA 2251755A1 CA 002251755 A CA002251755 A CA 002251755A CA 2251755 A CA2251755 A CA 2251755A CA 2251755 A1 CA2251755 A1 CA 2251755A1
- Authority
- CA
- Canada
- Prior art keywords
- substrate
- group
- selected regions
- functional groups
- coupling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
- C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00351—Means for dispensing and evacuation of reagents
- B01J2219/00427—Means for dispensing and evacuation of reagents using masks
- B01J2219/00432—Photolithographic masks
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00497—Features relating to the solid phase supports
- B01J2219/00527—Sheets
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00497—Features relating to the solid phase supports
- B01J2219/00527—Sheets
- B01J2219/00529—DNA chips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00585—Parallel processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/0059—Sequential processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00596—Solid-phase processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00608—DNA chips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/0061—The surface being organic
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00614—Delimitation of the attachment areas
- B01J2219/00621—Delimitation of the attachment areas by physical means, e.g. trenches, raised areas
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00623—Immobilisation or binding
- B01J2219/00626—Covalent
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00632—Introduction of reactive groups to the surface
- B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00659—Two-dimensional arrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/0068—Means for controlling the apparatus of the process
- B01J2219/00686—Automatic
- B01J2219/00689—Automatic using computers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00709—Type of synthesis
- B01J2219/00711—Light-directed synthesis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00722—Nucleotides
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00725—Peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B2200/00—Indexing scheme relating to specific properties of organic compounds
- C07B2200/11—Compounds covalently bound to a solid support
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
- C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/809—Multifield plates or multicontainer arrays
Abstract
The present invention provides novel processes for the large scale preparation of arrays of polymer sequences wherein each array includes a plurality of different, positionally distinct polymer sequences having known monomer sequences. The methods of the invention combine high throughput process steps with high resolution photolithographic techniques in the manufacture of polymer arrays.
Claims (49)
1. A method of forming an array of polymers on a surface of a substrate, comprising:
providing a substrate having a first surface coated with functional groups protected with a photolabile protecting group, and a second surface having a layer disposed thereon, said layer including one or more of an index matching compound, a light absorbing compound and an antireflective compound; and sequentially activating and coupling monomers in different selected regions of said substrate to form a plurality of different polymer sequences in different known locations on said surface of said substrate, wherein said activating step comprises directing an activation radiation at said first surface of said substrate.
providing a substrate having a first surface coated with functional groups protected with a photolabile protecting group, and a second surface having a layer disposed thereon, said layer including one or more of an index matching compound, a light absorbing compound and an antireflective compound; and sequentially activating and coupling monomers in different selected regions of said substrate to form a plurality of different polymer sequences in different known locations on said surface of said substrate, wherein said activating step comprises directing an activation radiation at said first surface of said substrate.
2. The method according to claim 1, wherein said layer disposed upon said second surface of said substrate comprises an index matching compound.
3. The method according to claim 1, wherein said layer disposed upon said second surface of said substrate is an antireflective compound.
4. The method according to claim 1, wherein said layer disposed upon said second surface of said substrate comprises an index matching compound and a light absorbing compound.
5. The method according to claim 1, wherein said layer disposed upon said second surface of said substrate comprises polyimide.
6. The method according to claim 1, wherein said plurality of different polymer sequences comprises a plurality of different oligonucleotide sequences.
7. A method of forming a plurality of individual polymer arrays, comprising:
providing a plurality of substrate wafers, each of said substrate wafers having a first surface, said first surface having functional groups disposed thereon, said functional groups being protected with a removable protecting group;
exposing a plurality of first selected regions on said first surface of each of said substrate wafers to an activator at an activation station to remove said removable protecting groups from said functional groups in said first selected regions;
first moving said substrate wafers batch-wise to a coupling station;
contacting said plurality of wafers with a monomer containing solution at said coupling station, to couple first selected monomers to said functional groups in said selected regions on said surface of said plurality of substrate wafers;
first returning said substrate wafers batch-wise to said activation station;
exposing a plurality of second selected regions on said first surface of each of said substrate wafers to an activator at said activation station to remove said removable protecting groups from said functional groups in said second selected regions;
second moving said substrate wafers batch-wise to said coupling station;
contacting said plurality of wafers with a monomer containing solution in said coupling station, to couple second selected monomers to said functional groups in said selected regions on said surface of said plurality of substrate wafers;
repeating said exposing and contacting steps to form a plurality of individual polymer arrays on said first surface of each of said substrate wafers.
providing a plurality of substrate wafers, each of said substrate wafers having a first surface, said first surface having functional groups disposed thereon, said functional groups being protected with a removable protecting group;
exposing a plurality of first selected regions on said first surface of each of said substrate wafers to an activator at an activation station to remove said removable protecting groups from said functional groups in said first selected regions;
first moving said substrate wafers batch-wise to a coupling station;
contacting said plurality of wafers with a monomer containing solution at said coupling station, to couple first selected monomers to said functional groups in said selected regions on said surface of said plurality of substrate wafers;
first returning said substrate wafers batch-wise to said activation station;
exposing a plurality of second selected regions on said first surface of each of said substrate wafers to an activator at said activation station to remove said removable protecting groups from said functional groups in said second selected regions;
second moving said substrate wafers batch-wise to said coupling station;
contacting said plurality of wafers with a monomer containing solution in said coupling station, to couple second selected monomers to said functional groups in said selected regions on said surface of said plurality of substrate wafers;
repeating said exposing and contacting steps to form a plurality of individual polymer arrays on said first surface of each of said substrate wafers.
8. The method according to claim 7, wherein said contacting steps comprise simultaneously contacting said plurality of substrate wafers with said monomer containing solutions.
9. The method according to claim 7, wherein said removable protecting group is a photolabile protecting group and said activator is light.
10. The method according to claim 7, wherein said exposing step comprises simultaneously contacting at least a first of said plurality of substrate wafers with a first monomer containing solution and contacting a second of said plurality of substrate wafers with a second monomer containing solution.
11. The method according to claim 7, wherein said activator comprises an activation radiation and said exposing step comprises directing said activation radiation at said first surface of said plurality of substrate wafers, and wherein a second surface of said plurality of substrate wafers includes a layer of one or more of an index matching compound, a light absorbing compound and an antireflective compound disposed thereon.
12. The method according to claim 11, wherein said layer disposed upon said second surface of said substrate comprises an index matching compound.
13. The method according to claim 11, wherein said layer disposed upon said second surface of said substrate is an antireflective compound.
14. The method according to claim 11, wherein said layer disposed upon said second surface of said substrate comprises an index matching compound and a light absorbing compound.
15. The method according to claim 11, wherein said layer disposed upon said second surface of said substrate comprises polyimide.
16. The method according to claim 7, wherein said plurality of different polymer sequences comprises a plurality of different oligonucleotide sequences.
17. A method of forming an array of polymers on a surface of a substrate, comprising:
derivatizing said surface of said substrate by contacting said surface of said substrate with a solution of aminoalkyltrialkoxysilane to provide amine functional groups on said first surface of said substrate;
protecting said functional groups with a protecting group;
activating first selected regions on said first surface of said substrate by removing said protecting groups from said functional groups in said first selected regions;
coupling a first monomer to said functional groups in said first selected regions;
activating second selected regions on said first surface of said substrate by removing said protecting groups from said functional groups in said second selected regions;
coupling a second monomer to said functional groups in said second selected regions;
repeating said activating and coupling steps to form a plurality of different polymer sequences, each of said different polymer sequences being coupled to said surface of said substrate in a different known location.
derivatizing said surface of said substrate by contacting said surface of said substrate with a solution of aminoalkyltrialkoxysilane to provide amine functional groups on said first surface of said substrate;
protecting said functional groups with a protecting group;
activating first selected regions on said first surface of said substrate by removing said protecting groups from said functional groups in said first selected regions;
coupling a first monomer to said functional groups in said first selected regions;
activating second selected regions on said first surface of said substrate by removing said protecting groups from said functional groups in said second selected regions;
coupling a second monomer to said functional groups in said second selected regions;
repeating said activating and coupling steps to form a plurality of different polymer sequences, each of said different polymer sequences being coupled to said surface of said substrate in a different known location.
18. The method according to claim 17, wherein said first and second monomers coupled in said coupling steps comprise an active phosphoramidite group.
19. The method according to claim 17, wherein said first and second monomers comprise nucleosides.
20. The method according to claim 17, wherein said aminoalkyltrialkoxysilane is selected from the group consisting of 3-aminopropyltriethoxysilane and 3-aminopropyltrimethoxysilane.
21. The method according to claim 17, wherein in said derivatizing step, said contacting of said surface of said substrate with a solution of aminoalkyltrialkoxysilane is carried out by controlled vapor deposition of said aminoalkyltrialkoxysilane on said surface.
22. The method according to claim 17, wherein said plurality of different polymer sequences comprises a plurality of different oligonucleotide sequences.
23. A method of forming an array of polymers on a surface of a substrate, comprising:
stripping said surface of said substrate with a stripping solution;
providing functional groups on said surface of said substrate;
protecting said functional groups with a photolabile protecting group;
exposing first selected regions on said first surface of said substrate to an activation radiation to remove said photolabile protecting groups from said functional groups in said first selected regions;
coupling a first monomer to said functional groups in said first selected regions;
exposing second selected regions on said first surface of said substrate wafers to an activation radiation to remove said photolabile protecting groups from said functional groups in said second selected regions;
coupling a second monomer to said functional groups in said second selected regions;
repeating said exposing and coupling steps to form a plurality of different polymer sequences, each of said different polymer sequences being coupled to said surface of said substrate in a different known location.
stripping said surface of said substrate with a stripping solution;
providing functional groups on said surface of said substrate;
protecting said functional groups with a photolabile protecting group;
exposing first selected regions on said first surface of said substrate to an activation radiation to remove said photolabile protecting groups from said functional groups in said first selected regions;
coupling a first monomer to said functional groups in said first selected regions;
exposing second selected regions on said first surface of said substrate wafers to an activation radiation to remove said photolabile protecting groups from said functional groups in said second selected regions;
coupling a second monomer to said functional groups in said second selected regions;
repeating said exposing and coupling steps to form a plurality of different polymer sequences, each of said different polymer sequences being coupled to said surface of said substrate in a different known location.
24. The method according to claim 23, wherein said stripping step comprises contacting said surface of said substrate with a stripping solution of concentrated NaOH/H2O2.
25. The method according to claim 23, wherein said stripping step further comprises:
contacting said surface of said substrate with base;
and rinsing said substrate with acid.
contacting said surface of said substrate with base;
and rinsing said substrate with acid.
26. A method of coupling monomers to selected regions on a surface of a substrate, comprising:
providing functional groups on said surface of said substrate, said functional groups being protected with a photoprotecting group;
exposing said selected regions to an activation radiation to remove said photolabile protecting group in said selected regions, said exposing step comprising directing an activation radiation at said selected regions on said surface of said substrate by shining said activation radiation through a photolithographic mask, said photolithographic mask including transparent regions and opaque regions, said transparent regions being smaller than said selected regions, whereby when said activation radiation is shone through said transparent regions in said mask, said activation radiation is diffracted to expose substantially all of said selected regions; and coupling monomers to said functional groups in said selected regions.
providing functional groups on said surface of said substrate, said functional groups being protected with a photoprotecting group;
exposing said selected regions to an activation radiation to remove said photolabile protecting group in said selected regions, said exposing step comprising directing an activation radiation at said selected regions on said surface of said substrate by shining said activation radiation through a photolithographic mask, said photolithographic mask including transparent regions and opaque regions, said transparent regions being smaller than said selected regions, whereby when said activation radiation is shone through said transparent regions in said mask, said activation radiation is diffracted to expose substantially all of said selected regions; and coupling monomers to said functional groups in said selected regions.
27. The method according to claim 26, wherein said transparent regions in said mask are from about 2% to about 25% smaller in each dimension than said selected regions.
28. The method according to claim 26, wherein said transparent regions in said mask are from about 10% to about 20% smaller in each dimension than said selected regions.
29. A method of forming polymer sequences on a solid substrate, comprising:
providing functional groups on said surface of said substrate;
coupling monomers to said functional groups on said surface of said substrate, said monomers having chemical groups reversibly coupled thereto, whereby said chemical groups enhance the lipophilicity of said monomers.
providing functional groups on said surface of said substrate;
coupling monomers to said functional groups on said surface of said substrate, said monomers having chemical groups reversibly coupled thereto, whereby said chemical groups enhance the lipophilicity of said monomers.
30. The method according to claim 29, wherein said monomer comprises a nucleotide protected with a lipophilic protecting group.
31. The method according to claim 30, wherein said lipophilic protecting group is coupled to an exocyclic functional group in a nucleobase in said nucleotide.
32. The method according to claim 31, wherein said exocyclic functional group is protected with a DMT protecting group.
33. The method according to claim 30, wherein said lipophilic protecting group is a photolabile protecting group.
34. The method according to claim 30, wherein said monomer comprises a nucleoside Fmoc-phosphoramidite.
35. A method of deprotecting an array of polymer sequences synthesized on a solid support, comprising contacting said array with a solution containing a substituted alkylamine.
36. The method according to claim 35, wherein said substituted alkylamine is selected from the group consisting of ethanolamine and ethylenediamine.
37. The method according to claim 35, wherein said solution of substituted alkylamine is a solution of ethylenediamine in ethanol.
38. The method according to claim 35, wherein said solution containing alkylamine is a 1:1 solution of ethylenediamine in ethanol.
39. A method of forming an array of polymer sequences on a surface of a substrate by sequentially deprotecting and coupling monomers in selected regions of said surface of said substrate to produce a plurality of different polymer sequences in different known locations of said surface of said substrate, the method comprising aligning deprotection and coupling steps in adjacent selected regions to minimize a number of differential synthesis steps between said adjacent regions.
40. A method of forming an array of polymer sequences wherein each polymer sequence has a subsequence of monomers common to a sequence that is complementary to a target sequence, but wherein at least one position within said subsequence is substituted with each member of a basis set of monomers, the method comprising coupling all monomers in a same layer of a first of said polymer sequences in a same synthesis cycle as corresponding monomers in a second of said polymer sequences.
41. A method of photoprotecting a functional group coupled to a solid support, the method comprising exposing said functional group to a photoprotecting group transfer agent having the formula:
wherein R1 is a photolabile protecting group and X is a leaving group.
wherein R1 is a photolabile protecting group and X is a leaving group.
42. The method according to claim 41, wherein X is selected from the group consisting of:
where R2 is alkyl, substituted alkyl or aryl, R3 is hydrogen, alkyl, thioalkyl, aryl; R4 is an electron withdrawing group;
R5 is a sterically hindered alkyl or aryl group; and R6 is alkyl or aryl group said alkyl or aryl group comprising an electronegative substituent.
where R2 is alkyl, substituted alkyl or aryl, R3 is hydrogen, alkyl, thioalkyl, aryl; R4 is an electron withdrawing group;
R5 is a sterically hindered alkyl or aryl group; and R6 is alkyl or aryl group said alkyl or aryl group comprising an electronegative substituent.
43. The method according to claim 42, wherein R4 is selected from the group consisting of NO2, SO2-R2, and CN.
44 . The method according to claim 42, wherein R5 is selected from the group consisting of adamantyl and t-butyl.
45. The method according to claim 42, wherein said electronegative substituent is selected from the group consisting of:
46. The method according to claim 41, wherein R1 is selected from the group consisting of NVOC, NPOC, MeNVOC, MeNPOC, PYMOC, NV, NP, MeNV and MeNP.
47. The method according to claim 41, wherein said exposing step is carried out in the presence of a nonnucleophilic organic solvent and a base catalyst.
48. The method according to claim 47, wherein said nonnucleophilic organic solvent is selected from the group consisting of DMF, NMP, DCM, THF and ACN.
49. The method according to claim 47, wherein said base catalyst is selected from the group consisting of pyridine, 2,6-lutidine, TEA and DIEA.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/634,053 US5959098A (en) | 1996-04-17 | 1996-04-17 | Substrate preparation process |
US08/634,053 | 1996-04-17 | ||
PCT/US1997/006535 WO1997039151A1 (en) | 1996-04-17 | 1997-04-16 | Photolabile polymer array synthesis methods |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2251755A1 true CA2251755A1 (en) | 1997-10-23 |
CA2251755C CA2251755C (en) | 2010-09-14 |
Family
ID=24542253
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2251755A Expired - Lifetime CA2251755C (en) | 1996-04-17 | 1997-04-16 | Photolabile polymer array synthesis methods |
Country Status (6)
Country | Link |
---|---|
US (6) | US5959098A (en) |
EP (1) | EP0950112A4 (en) |
JP (2) | JP2000508542A (en) |
AU (1) | AU2804797A (en) |
CA (1) | CA2251755C (en) |
WO (1) | WO1997039151A1 (en) |
Families Citing this family (325)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5959098A (en) | 1996-04-17 | 1999-09-28 | Affymetrix, Inc. | Substrate preparation process |
US6147205A (en) * | 1995-12-15 | 2000-11-14 | Affymetrix, Inc. | Photocleavable protecting groups and methods for their use |
US20110028350A1 (en) * | 1995-12-15 | 2011-02-03 | Affymetrix, Inc. | Photocleavable protecting groups |
US6706875B1 (en) * | 1996-04-17 | 2004-03-16 | Affyemtrix, Inc. | Substrate preparation process |
CA2255599C (en) | 1996-04-25 | 2006-09-05 | Bioarray Solutions, Llc | Light-controlled electrokinetic assembly of particles near surfaces |
ATE225858T1 (en) | 1997-07-28 | 2002-10-15 | Medical Biosystems Ltd | SEQUENCE ANALYSIS OF NUCLEIC ACIDS |
NO306357B1 (en) * | 1997-10-16 | 1999-10-25 | Torstein Ljungmann | Color machine for preparation of tissue samples placed on slides |
US6251588B1 (en) * | 1998-02-10 | 2001-06-26 | Agilent Technologies, Inc. | Method for evaluating oligonucleotide probe sequences |
US6426184B1 (en) * | 1998-02-11 | 2002-07-30 | The Regents Of The University Of Michigan | Method and apparatus for chemical and biochemical reactions using photo-generated reagents |
AU772531B2 (en) * | 1998-02-11 | 2004-04-29 | University Of Houston, The | Method and apparatus for chemical and biochemical reactions using photo-generated reagents |
JP4698023B2 (en) * | 1998-02-23 | 2011-06-08 | ウイスコンシン アラムニ リサーチ ファンデーション | Method and apparatus for synthesizing DNA probe array |
US6235480B1 (en) * | 1998-03-13 | 2001-05-22 | Promega Corporation | Detection of nucleic acid hybrids |
US6391005B1 (en) | 1998-03-30 | 2002-05-21 | Agilent Technologies, Inc. | Apparatus and method for penetration with shaft having a sensor for sensing penetration depth |
WO1999054509A1 (en) * | 1998-04-20 | 1999-10-28 | Affymetrix, Inc. | Methods for reducing non-specific binding to a nucleic acid probe array |
US6979728B2 (en) * | 1998-05-04 | 2005-12-27 | Baylor College Of Medicine | Articles of manufacture and methods for array based analysis of biological molecules |
US6048695A (en) * | 1998-05-04 | 2000-04-11 | Baylor College Of Medicine | Chemically modified nucleic acids and methods for coupling nucleic acids to solid support |
US6271957B1 (en) * | 1998-05-29 | 2001-08-07 | Affymetrix, Inc. | Methods involving direct write optical lithography |
WO1999063385A1 (en) * | 1998-06-04 | 1999-12-09 | Board Of Regents, The University Of Texas System | Digital optical chemistry micromirror imager |
ATE423314T1 (en) | 1998-06-24 | 2009-03-15 | Illumina Inc | DECODING OF MATRIXED SENSORS BY MICROPARTICLES |
US7097974B1 (en) | 1998-08-28 | 2006-08-29 | Febit Biotech Gmbh | Support for a method for determining an analyte and a method for producing the support |
DE19842164B4 (en) * | 1998-09-15 | 2004-06-03 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | Quality control procedures for the construction of oligomer grids |
US6703228B1 (en) | 1998-09-25 | 2004-03-09 | Massachusetts Institute Of Technology | Methods and products related to genotyping and DNA analysis |
US6262216B1 (en) | 1998-10-13 | 2001-07-17 | Affymetrix, Inc. | Functionalized silicon compounds and methods for their synthesis and use |
US6951682B1 (en) * | 1998-12-01 | 2005-10-04 | Syntrix Biochip, Inc. | Porous coatings bearing ligand arrays and use thereof |
GB9906929D0 (en) * | 1999-03-26 | 1999-05-19 | Univ Glasgow | Assay system |
US6824866B1 (en) | 1999-04-08 | 2004-11-30 | Affymetrix, Inc. | Porous silica substrates for polymer synthesis and assays |
AU4207600A (en) * | 1999-04-08 | 2000-11-14 | Affymetrix, Inc. | Porous silica substrates for polymers synthesis and assays |
US20020177135A1 (en) * | 1999-07-27 | 2002-11-28 | Doung Hau H. | Devices and methods for biochip multiplexing |
WO2000079006A1 (en) * | 1999-06-17 | 2000-12-28 | Fred Hutchinson Cancer Research Center | Oligonucleotide arrays for high resolution hla typing |
JP3471664B2 (en) * | 1999-07-08 | 2003-12-02 | Nec液晶テクノロジー株式会社 | Cutting device for bonded substrates for liquid crystal cells |
US6750023B2 (en) * | 1999-09-02 | 2004-06-15 | Corning Incorporated | Porous inorganic substrate for high-density arrays |
EP1212461A4 (en) * | 1999-09-02 | 2006-10-18 | Corning Inc | Porous substrates for dna arrays |
US6994972B2 (en) * | 1999-09-02 | 2006-02-07 | Corning Incorporated | Porous substrates for DNA arrays |
US7115423B1 (en) * | 1999-10-22 | 2006-10-03 | Agilent Technologies, Inc. | Fluidic structures within an array package |
JP2003517589A (en) * | 1999-11-02 | 2003-05-27 | セリーヌ フー, | Molecular microarrays and methods for their production and use |
US6784982B1 (en) * | 1999-11-04 | 2004-08-31 | Regents Of The University Of Minnesota | Direct mapping of DNA chips to detector arrays |
US6867851B2 (en) * | 1999-11-04 | 2005-03-15 | Regents Of The University Of Minnesota | Scanning of biological samples |
US6642046B1 (en) | 1999-12-09 | 2003-11-04 | Motorola, Inc. | Method and apparatus for performing biological reactions on a substrate surface |
US6800439B1 (en) | 2000-01-06 | 2004-10-05 | Affymetrix, Inc. | Methods for improved array preparation |
US6458526B1 (en) | 2000-01-28 | 2002-10-01 | Agilent Technologies, Inc. | Method and apparatus to inhibit bubble formation in a fluid |
AU4116401A (en) * | 2000-03-16 | 2001-09-24 | Matsushita Electric Industrial Co., Ltd. | Nucleotide detector, process for producing the same and process for forming fineparticle membrane |
US6806361B1 (en) | 2000-03-17 | 2004-10-19 | Affymetrix, Inc. | Methods of enhancing functional performance of nucleic acid arrays |
US6833450B1 (en) | 2000-03-17 | 2004-12-21 | Affymetrix, Inc. | Phosphite ester oxidation in nucleic acid array preparation |
EP1284421A4 (en) * | 2000-05-12 | 2005-12-28 | Kyowa Hakko Kogyo Kk | Method of constructing polynucleotide microarray, apparatus for the construction and polynucleotide microarray |
US7005259B1 (en) * | 2000-06-01 | 2006-02-28 | Affymetrix, Inc. | Methods for array preparation using substrate rotation |
US7892854B2 (en) | 2000-06-21 | 2011-02-22 | Bioarray Solutions, Ltd. | Multianalyte molecular analysis using application-specific random particle arrays |
US9709559B2 (en) | 2000-06-21 | 2017-07-18 | Bioarray Solutions, Ltd. | Multianalyte molecular analysis using application-specific random particle arrays |
US20040161789A1 (en) * | 2000-08-30 | 2004-08-19 | Tanner Cameron W. | Porous inorganic substrate for high-density arrays |
AU2001292142A1 (en) * | 2000-09-11 | 2002-03-22 | Affymetrix, Inc. | Photocleavable protecting groups |
GB0022978D0 (en) | 2000-09-19 | 2000-11-01 | Oxford Glycosciences Uk Ltd | Detection of peptides |
US20020037359A1 (en) * | 2000-09-25 | 2002-03-28 | Mutz Mitchell W. | Focused acoustic energy in the preparation of peptide arrays |
US6864097B1 (en) | 2000-09-27 | 2005-03-08 | Agilent Technologies, Inc. | Arrays and their reading |
US20060141507A1 (en) * | 2000-09-27 | 2006-06-29 | Kronick Mel N | Manufacture and use of non-standard size microarray slides |
DE10051396A1 (en) | 2000-10-17 | 2002-04-18 | Febit Ferrarius Biotech Gmbh | An integrated synthesis and identification of an analyte, comprises particles immobilized at a carrier to be coupled to receptors in a structured pattern to give receptor arrays for biochemical reactions |
US6835534B2 (en) | 2000-10-27 | 2004-12-28 | The Penn State Research Foundation | Chemical functionalization nanolithography |
US6528264B1 (en) * | 2000-11-01 | 2003-03-04 | Corning Incorporated | Polymer support for DNA immobilization |
US20020150887A1 (en) * | 2000-11-09 | 2002-10-17 | National Institute Of Advanced Industrial Science And Technology | Methods and nucleic acid probes for molecular genetic analysis of polluted environments and environmental samples |
US8641644B2 (en) | 2000-11-21 | 2014-02-04 | Sanofi-Aventis Deutschland Gmbh | Blood testing apparatus having a rotatable cartridge with multiple lancing elements and testing means |
US7117095B2 (en) * | 2000-11-21 | 2006-10-03 | Affymetrix, Inc. | Methods for selecting nucleic acid probes |
US20020075490A1 (en) * | 2000-12-20 | 2002-06-20 | Affymetrix, Inc. | System and method for addressable light-directed microarray printing |
WO2002052045A1 (en) * | 2000-12-26 | 2002-07-04 | Aviva Biosciences | Active and biocompatible platforms prepared by polymerization of surface coating films |
EP1623762A3 (en) * | 2000-12-28 | 2014-05-21 | F.Hoffmann-La Roche Ag | Cartridge for processing a nucleic acid sample |
US6399713B1 (en) | 2001-01-24 | 2002-06-04 | Arizona Chemical Company | Hydrocarbon-terminated polyether-polyamide block copolymers and uses thereof |
US6870011B2 (en) | 2001-01-24 | 2005-03-22 | Arizona Chemical Company | Hydrocarbon-terminated polyether-polyamide block copolymers and uses thereof |
WO2002100254A2 (en) | 2001-06-12 | 2002-12-19 | Pelikan Technologies, Inc. | Method and apparatus for lancet launching device integrated onto a blood-sampling cartridge |
US7981056B2 (en) | 2002-04-19 | 2011-07-19 | Pelikan Technologies, Inc. | Methods and apparatus for lancet actuation |
US9427532B2 (en) | 2001-06-12 | 2016-08-30 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
DE60238119D1 (en) | 2001-06-12 | 2010-12-09 | Pelikan Technologies Inc | ELECTRIC ACTUATOR ELEMENT FOR A LANZETTE |
US8337419B2 (en) | 2002-04-19 | 2012-12-25 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
US9795747B2 (en) | 2010-06-02 | 2017-10-24 | Sanofi-Aventis Deutschland Gmbh | Methods and apparatus for lancet actuation |
US7025774B2 (en) | 2001-06-12 | 2006-04-11 | Pelikan Technologies, Inc. | Tissue penetration device |
ES2336081T3 (en) | 2001-06-12 | 2010-04-08 | Pelikan Technologies Inc. | SELF-OPTIMIZATION PUNCTURE DEVICE WITH MEANS OF ADAPTATION TO TEMPORARY VARIATIONS IN CUTANEOUS PROPERTIES. |
US9226699B2 (en) | 2002-04-19 | 2016-01-05 | Sanofi-Aventis Deutschland Gmbh | Body fluid sampling module with a continuous compression tissue interface surface |
US7262063B2 (en) | 2001-06-21 | 2007-08-28 | Bio Array Solutions, Ltd. | Directed assembly of functional heterostructures |
US6649348B2 (en) * | 2001-06-29 | 2003-11-18 | Agilent Technologies Inc. | Methods for manufacturing arrays |
US10272409B2 (en) | 2001-07-11 | 2019-04-30 | Michael E. Hogan | Methods and devices based upon a novel form of nucleic acid duplex on a surface |
JP4312595B2 (en) * | 2001-07-11 | 2009-08-12 | ベイラー カレッジ オブ メディシン | Method and apparatus based on a novel form of nucleic acid duplex on a surface |
WO2003006676A2 (en) * | 2001-07-13 | 2003-01-23 | Nanosphere, Inc. | Method for immobilizing molecules onto surfaces |
US7297553B2 (en) | 2002-05-28 | 2007-11-20 | Nanosphere, Inc. | Method for attachment of silylated molecules to glass surfaces |
US6916541B2 (en) * | 2001-09-07 | 2005-07-12 | Penn State Research Foundation | Modified substrates for the attachment of biomolecules |
WO2003033128A2 (en) * | 2001-10-12 | 2003-04-24 | Duke University | Methods for image analysis of high-density synthetic dna microarrays |
CA2463725A1 (en) | 2001-10-12 | 2003-11-06 | Spectral Genomics, Inc. | Compilations of nucleic acids and arrays and methods of using them |
US20030087289A1 (en) * | 2001-10-12 | 2003-05-08 | Harry Zuzan | Image analysis of high-density synthetic DNA microarrays |
US7439346B2 (en) | 2001-10-12 | 2008-10-21 | Perkinelmer Las Inc. | Nucleic acids arrays and methods of use therefor |
KR20040068122A (en) | 2001-10-15 | 2004-07-30 | 바이오어레이 솔루션스 리미티드 | Multiplexed analysis of polymorphic loci by concurrent interrogation and enzyme-mediated detection |
US20030073089A1 (en) * | 2001-10-16 | 2003-04-17 | Mauze Ganapati R. | Companion cartridge for disposable diagnostic sensing platforms |
CN1281324C (en) * | 2001-12-19 | 2006-10-25 | 阿菲梅特里克斯公司 | Manufacturing process for array plate assembly |
US7335153B2 (en) * | 2001-12-28 | 2008-02-26 | Bio Array Solutions Ltd. | Arrays of microparticles and methods of preparation thereof |
US7422851B2 (en) * | 2002-01-31 | 2008-09-09 | Nimblegen Systems, Inc. | Correction for illumination non-uniformity during the synthesis of arrays of oligomers |
US7083975B2 (en) * | 2002-02-01 | 2006-08-01 | Roland Green | Microarray synthesis instrument and method |
US20030162283A1 (en) * | 2002-02-22 | 2003-08-28 | Hitachi, Ltd. | Circulating type biochemical reaction apparatus |
US6916621B2 (en) | 2002-03-27 | 2005-07-12 | Spectral Genomics, Inc. | Methods for array-based comparitive binding assays |
US6773888B2 (en) | 2002-04-08 | 2004-08-10 | Affymetrix, Inc. | Photoactivatable silane compounds and methods for their synthesis and use |
US7226771B2 (en) | 2002-04-19 | 2007-06-05 | Diversa Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
US7547287B2 (en) | 2002-04-19 | 2009-06-16 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US7892183B2 (en) | 2002-04-19 | 2011-02-22 | Pelikan Technologies, Inc. | Method and apparatus for body fluid sampling and analyte sensing |
US8221334B2 (en) | 2002-04-19 | 2012-07-17 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
EP1497418B1 (en) | 2002-04-19 | 2012-10-17 | Verenium Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
US8702624B2 (en) | 2006-09-29 | 2014-04-22 | Sanofi-Aventis Deutschland Gmbh | Analyte measurement device with a single shot actuator |
US7674232B2 (en) | 2002-04-19 | 2010-03-09 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US7331931B2 (en) | 2002-04-19 | 2008-02-19 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US8372016B2 (en) | 2002-04-19 | 2013-02-12 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for body fluid sampling and analyte sensing |
US7909778B2 (en) | 2002-04-19 | 2011-03-22 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US8784335B2 (en) | 2002-04-19 | 2014-07-22 | Sanofi-Aventis Deutschland Gmbh | Body fluid sampling device with a capacitive sensor |
US8360992B2 (en) | 2002-04-19 | 2013-01-29 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
US9314194B2 (en) | 2002-04-19 | 2016-04-19 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
US8579831B2 (en) | 2002-04-19 | 2013-11-12 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
US7901362B2 (en) | 2002-04-19 | 2011-03-08 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US7229458B2 (en) | 2002-04-19 | 2007-06-12 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US7175642B2 (en) | 2002-04-19 | 2007-02-13 | Pelikan Technologies, Inc. | Methods and apparatus for lancet actuation |
US7708701B2 (en) | 2002-04-19 | 2010-05-04 | Pelikan Technologies, Inc. | Method and apparatus for a multi-use body fluid sampling device |
US7232451B2 (en) | 2002-04-19 | 2007-06-19 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US9248267B2 (en) | 2002-04-19 | 2016-02-02 | Sanofi-Aventis Deustchland Gmbh | Tissue penetration device |
US7297122B2 (en) | 2002-04-19 | 2007-11-20 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US8267870B2 (en) | 2002-04-19 | 2012-09-18 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for body fluid sampling with hybrid actuation |
US7491178B2 (en) | 2002-04-19 | 2009-02-17 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US7976476B2 (en) | 2002-04-19 | 2011-07-12 | Pelikan Technologies, Inc. | Device and method for variable speed lancet |
US9795334B2 (en) | 2002-04-19 | 2017-10-24 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
US7217512B2 (en) * | 2002-05-09 | 2007-05-15 | Corning Incorporated | Reagent and method for attaching target molecules to a surface |
KR100451663B1 (en) * | 2002-05-15 | 2004-10-08 | 한국전자통신연구원 | Programmable mask and a method for fabricating biomolecule array using the same |
US20070275411A1 (en) * | 2006-05-25 | 2007-11-29 | Mcgall Glenn H | Silane mixtures |
US7332273B2 (en) * | 2002-06-20 | 2008-02-19 | Affymetrix, Inc. | Antireflective coatings for high-resolution photolithographic synthesis of DNA arrays |
US7220573B2 (en) * | 2002-06-21 | 2007-05-22 | Agilent Technologies, Inc. | Array assay devices and methods of using the same |
WO2004003233A1 (en) * | 2002-06-28 | 2004-01-08 | Rosetta Inpharmatics Llc | Methods to assess quality of microarrays |
US7504215B2 (en) | 2002-07-12 | 2009-03-17 | Affymetrix, Inc. | Nucleic acid labeling methods |
US20040101444A1 (en) * | 2002-07-15 | 2004-05-27 | Xeotron Corporation | Apparatus and method for fluid delivery to a hybridization station |
US7452712B2 (en) | 2002-07-30 | 2008-11-18 | Applied Biosystems Inc. | Sample block apparatus and method of maintaining a microcard on a sample block |
GB0217963D0 (en) * | 2002-08-02 | 2002-09-11 | Cyclops Genome Sciences Ltd | Purification of nucleic acids |
US20040142864A1 (en) * | 2002-09-16 | 2004-07-22 | Plexxikon, Inc. | Crystal structure of PIM-1 kinase |
DE10393431T5 (en) | 2002-10-01 | 2005-11-17 | Nimblegen Systems, Inc., Madison | Microarrays with multiple oligonucleotides in single array features |
US20040076961A1 (en) * | 2002-10-21 | 2004-04-22 | Lewis Mark A. | Biomolecule retaining material and methods for attaching biomolecules to a surface |
US7195908B2 (en) * | 2002-10-31 | 2007-03-27 | Corning Incorporated | Supports treated with triamine for immobilizing biomolecules |
US7526114B2 (en) | 2002-11-15 | 2009-04-28 | Bioarray Solutions Ltd. | Analysis, secure access to, and transmission of array images |
US20040137606A1 (en) * | 2002-12-11 | 2004-07-15 | Jung Moon Youn | Programmable mask for forming biomolecule or polymer array and fabrication method of biomolecule or polymer array using the same |
US20040180368A1 (en) * | 2002-12-23 | 2004-09-16 | Affymetrix, Inc. | Method for producing a high density nucleic acid array using activators |
US8574895B2 (en) | 2002-12-30 | 2013-11-05 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus using optical techniques to measure analyte levels |
US20050048573A1 (en) * | 2003-02-03 | 2005-03-03 | Plexxikon, Inc. | PDE5A crystal structure and uses |
US20040152085A1 (en) * | 2003-02-04 | 2004-08-05 | Veridian Systems Division | Surface for collection and/or purification of nucleic acids |
US7223851B2 (en) * | 2003-02-06 | 2007-05-29 | General Dynamics Advanced Information Systems, Inc. | Nucleic acid-binding polymers |
US20050170431A1 (en) * | 2003-02-28 | 2005-08-04 | Plexxikon, Inc. | PYK2 crystal structure and uses |
US20040175504A1 (en) * | 2003-03-06 | 2004-09-09 | Zyomyx, Inc. | Substrate processing apparatus and method for the controlled formation of layers including self-assembled monolayers |
EP3023498B1 (en) | 2003-03-06 | 2018-11-28 | BASF Enzymes LLC | Amylases, nucleic acids encoding them and methods for making and using them |
MX295545B (en) | 2003-03-07 | 2012-02-03 | Diversa Corp | Hydrolases, nucleic acids encoding them and mehods for making and using them. |
JP2004279196A (en) * | 2003-03-14 | 2004-10-07 | Dainippon Printing Co Ltd | Substrate for bio-micro-array and bio-micro-array |
US7592434B2 (en) | 2003-04-04 | 2009-09-22 | Verenium Corporation | Pectate lyases, nucleic encoding them and methods for making and using them |
ATE476137T1 (en) | 2003-05-30 | 2010-08-15 | Pelikan Technologies Inc | METHOD AND DEVICE FOR INJECTING LIQUID |
DK1633235T3 (en) | 2003-06-06 | 2014-08-18 | Sanofi Aventis Deutschland | Apparatus for sampling body fluid and detecting analyte |
CA3084542A1 (en) | 2003-06-10 | 2005-01-06 | The Trustees Of Boston University | Gene expression analysis of airway epithelial cells for diagnosing lung cancer |
WO2006001797A1 (en) | 2004-06-14 | 2006-01-05 | Pelikan Technologies, Inc. | Low pain penetrating |
EP2404931A1 (en) | 2003-07-02 | 2012-01-11 | Verenium Corporation | Glucanases, nucleic acids encoding them and methods for making and using them |
US20050079548A1 (en) * | 2003-07-07 | 2005-04-14 | Plexxikon, Inc. | Ligand development using PDE4B crystal structures |
US20050048571A1 (en) * | 2003-07-29 | 2005-03-03 | Danielson Paul S. | Porous glass substrates with reduced auto-fluorescence |
CA2535526C (en) | 2003-08-11 | 2015-09-29 | Diversa Corporation | Laccases, nucleic acids encoding them and methods for making and using them |
WO2005028624A2 (en) * | 2003-09-15 | 2005-03-31 | Plexxikon, Inc. | Molecular scaffolds for kinase ligand development |
WO2005029705A2 (en) | 2003-09-18 | 2005-03-31 | Bioarray Solutions, Ltd. | Number coding for identification of subtypes of coded types of solid phase carriers |
CA2539824C (en) | 2003-09-22 | 2015-02-03 | Xinwen Wang | Surface immobilized polyelectrolyte with multiple functional groups capable of covalently bonding to biomolecules |
EP1671096A4 (en) | 2003-09-29 | 2009-09-16 | Pelikan Technologies Inc | Method and apparatus for an improved sample capture device |
EP1680014A4 (en) | 2003-10-14 | 2009-01-21 | Pelikan Technologies Inc | Method and apparatus for a variable user interface |
AU2004286252A1 (en) | 2003-10-28 | 2005-05-12 | Bioarray Solutions Ltd. | Optimization of gene expression analysis using immobilized capture probes |
WO2005045060A2 (en) | 2003-10-29 | 2005-05-19 | Bioarray Solutions, Ltd. | Multiplexed nucleic acid analysis by fragmentation of double-stranded dna |
AU2004286845A1 (en) * | 2003-10-30 | 2005-05-19 | Tufts-New England Medical Center | Prenatal diagnosis using cell-free fetal DNA in amniotic fluid |
FR2861608B1 (en) * | 2003-10-31 | 2005-12-23 | Commissariat Energie Atomique | WORKING DEVICE COMPRISING WORKED AREAS, LABORATORY ON CHIP AND MICROSYSTEM |
WO2005054516A2 (en) | 2003-11-26 | 2005-06-16 | Advandx, Inc. | Peptide nucleic acid probes for analysis of certain staphylococcus species |
US20070066641A1 (en) * | 2003-12-19 | 2007-03-22 | Prabha Ibrahim | Compounds and methods for development of RET modulators |
EP1696920B8 (en) * | 2003-12-19 | 2015-05-06 | Plexxikon Inc. | Compounds and methods for development of ret modulators |
CA2490675A1 (en) * | 2003-12-22 | 2005-06-22 | Affymetrix, Inc. | Process for high-yield synthesis of standard length and long-mer nucleic acid arrays |
US7822454B1 (en) | 2005-01-03 | 2010-10-26 | Pelikan Technologies, Inc. | Fluid sampling device with improved analyte detecting member configuration |
EP1706026B1 (en) | 2003-12-31 | 2017-03-01 | Sanofi-Aventis Deutschland GmbH | Method and apparatus for improving fluidic flow and sample capture |
EP1564306B1 (en) | 2004-02-17 | 2013-08-07 | Affymetrix, Inc. | Methods for fragmenting and labeling DNA |
US20050214777A1 (en) * | 2004-03-29 | 2005-09-29 | Peck Bill J | Methods for in situ generation of nucleic acid arrays |
US20050214778A1 (en) * | 2004-03-29 | 2005-09-29 | Peck Bill J | Methods for in situ generation of nucleic acid arrays |
US7384779B2 (en) * | 2004-04-12 | 2008-06-10 | Corning Incorporated | Porous substrate plates and the use thereof |
US7585859B2 (en) * | 2004-05-06 | 2009-09-08 | Plexxikon, Inc. | PDE4B inhibitors and uses therefor |
CA2567627C (en) * | 2004-05-20 | 2014-09-30 | Quest Diagnostics Investments Incorporated | Single label comparative hybridization |
US8828203B2 (en) | 2004-05-20 | 2014-09-09 | Sanofi-Aventis Deutschland Gmbh | Printable hydrogels for biosensors |
US9775553B2 (en) | 2004-06-03 | 2017-10-03 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for a fluid sampling device |
WO2005120365A1 (en) | 2004-06-03 | 2005-12-22 | Pelikan Technologies, Inc. | Method and apparatus for a fluid sampling device |
AU2005264938B2 (en) | 2004-06-16 | 2011-11-24 | Verenium Corporation | Compositions and methods for enzymatic decolorization of chlorophyll |
US7498342B2 (en) * | 2004-06-17 | 2009-03-03 | Plexxikon, Inc. | Compounds modulating c-kit activity |
US20060058339A1 (en) * | 2004-06-17 | 2006-03-16 | Ibrahim Prabha N | Compounds modulating c-kit activity and uses therefor |
JP4654627B2 (en) * | 2004-07-26 | 2011-03-23 | セイコーエプソン株式会社 | Chemical adsorption film forming method and chemical adsorption film |
FR2873697B1 (en) * | 2004-07-28 | 2006-10-27 | Commissariat Energie Atomique | PHOTOLABILE PROTECTIVE GROUPING COUPLING AGENTS AND THEIR USES, IN PARTICULAR FOR THE FUNCTIONALIZATION OF SOLID SUPPORTS |
US7848889B2 (en) | 2004-08-02 | 2010-12-07 | Bioarray Solutions, Ltd. | Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification |
KR20060012686A (en) | 2004-08-04 | 2006-02-09 | 한국전자통신연구원 | Programmable mask and a method for fabricating biomolecule array using the same |
EP1786813A2 (en) * | 2004-09-03 | 2007-05-23 | Plexxikon, Inc. | Bicyclic heteroaryl pde4b inhibitors |
US20060073506A1 (en) | 2004-09-17 | 2006-04-06 | Affymetrix, Inc. | Methods for identifying biological samples |
EP1645640B1 (en) | 2004-10-05 | 2013-08-21 | Affymetrix, Inc. | Method for detecting chromosomal translocations |
EP1652580A1 (en) | 2004-10-29 | 2006-05-03 | Affymetrix, Inc. | High throughput microarray, package assembly and methods of manufacturing arrays |
US7682782B2 (en) | 2004-10-29 | 2010-03-23 | Affymetrix, Inc. | System, method, and product for multiple wavelength detection using single source excitation |
JP4928075B2 (en) * | 2004-12-24 | 2012-05-09 | 日本曹達株式会社 | Organic thin film surface property conversion method |
US20060147943A1 (en) * | 2004-12-30 | 2006-07-06 | Lewis Mark A | Substrates having pendant epoxide groups for binding biomolecules and methods of making and using thereof |
US8652831B2 (en) | 2004-12-30 | 2014-02-18 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for analyte measurement test time |
US8338093B2 (en) * | 2004-12-31 | 2012-12-25 | Affymetrix, Inc. | Primer array synthesis and validation |
US7547775B2 (en) * | 2004-12-31 | 2009-06-16 | Affymetrix, Inc. | Parallel preparation of high fidelity probes in an array format |
DE602006018861D1 (en) * | 2005-01-27 | 2011-01-27 | Quest Diagnostics Invest Inc | FAST COMPARATIVE GENOM HYBRIDIZATION |
WO2006099207A2 (en) | 2005-03-10 | 2006-09-21 | Diversa Corporation | Lyase enzymes, nucleic acids encoding them and methods for making and using them |
NZ594810A (en) | 2005-03-15 | 2012-12-21 | Verenium Corp | Cellulases, nucleic acids encoding them and methods for making and using them |
US20080020941A1 (en) | 2005-03-24 | 2008-01-24 | Jimpei Tabata | Biomolecule-immobilized plate and method for fabricating biomolecule-immobilized plate |
EP2360278A1 (en) | 2005-04-14 | 2011-08-24 | Trustees Of Boston University | Diagnostic for lung disorders using class prediction |
KR20080027775A (en) * | 2005-05-17 | 2008-03-28 | 플렉시콘, 인코퍼레이티드 | Pyrrole(2,3-b)pyridine derivatives protein kinase inhibitors |
US8486629B2 (en) | 2005-06-01 | 2013-07-16 | Bioarray Solutions, Ltd. | Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation |
CN102603581B (en) * | 2005-06-22 | 2015-06-24 | 普莱希科公司 | Pyrrolo[2,3-b] pyridine derivatives as protein kinase inhibitors |
US20060292559A1 (en) * | 2005-06-23 | 2006-12-28 | Beckman Coulter, Inc. | Cell-based microarrays, and methods for their preparation and use |
WO2007016502A2 (en) | 2005-08-02 | 2007-02-08 | Wisconsin Alumni Research Foundation | Synthesis of arrays of oligonucleotides and other chain molecules |
US20070059742A1 (en) * | 2005-09-09 | 2007-03-15 | Stover Axel G | Process of stripping a microarray for reuse |
US20100233429A1 (en) * | 2005-09-16 | 2010-09-16 | Yamatake Corporation | Substrate for Biochip, Biochip, Method for Manufacturing Substrate for Biochip and Method for Manufacturing Biochip |
US8076074B2 (en) | 2005-11-29 | 2011-12-13 | Quest Diagnostics Investments Incorporated | Balanced translocation in comparative hybridization |
US7634363B2 (en) | 2005-12-07 | 2009-12-15 | Affymetrix, Inc. | Methods for high throughput genotyping |
US7760274B2 (en) * | 2005-12-08 | 2010-07-20 | Electronics And Telecommunications Research Institute | Programmable mask for fabricating biomolecule array or polymer array, apparatus for fabricating biomolecule array or polymer array including the programmable mask, and method of fabricating biomolecule array or polymer array using the programmable mask |
US7994098B2 (en) * | 2005-12-09 | 2011-08-09 | Wisconsin Alumni Research Foundation | Light directed DNA synthesis using inverse capping for error reduction |
US7862996B2 (en) | 2005-12-29 | 2011-01-04 | Affymetrix, Inc. | Use of acid scavengers for the synthesis of standard length and long-mer nucleic acid arrays |
US20070154946A1 (en) * | 2005-12-29 | 2007-07-05 | Rajasekaran John J | Massively parallel synthesis of biopolymeric arrays |
EP1987358A4 (en) * | 2006-01-26 | 2013-12-04 | California Inst Of Techn | Mechanically induced trapping of molecular interactions |
WO2007092314A2 (en) | 2006-02-02 | 2007-08-16 | Verenium Corporation | Esterases and related nucleic acids and methods |
DK2450439T3 (en) | 2006-02-10 | 2014-02-10 | Verenium Corp | Cellulose-degrading enzymes, nucleic acids encoding them, and methods of preparing and utilizing them |
EP2548955A1 (en) | 2006-02-14 | 2013-01-23 | Verenium Corporation | Xylanases, nucleic acids encoding them and methods for making and using them |
KR101588539B1 (en) | 2006-03-07 | 2016-01-25 | 바스프 엔자임스 엘엘씨 | Aldolases, nucleic acids encoding them and methods for making and using them |
EP2112227B1 (en) | 2006-03-07 | 2013-05-15 | Cargill, Incorporated | Aldolases, nucleic acids encoding them and methods for making and using them |
EP2605018A1 (en) | 2006-03-09 | 2013-06-19 | The Trustees of the Boston University | Diagnostic and prognostic methods for lung disorders using gene expression profiles from nose epithelial cells |
EP1996947A1 (en) * | 2006-03-24 | 2008-12-03 | The Regents of the University of Michigan | Method for forming molecular sequences on surfaces |
US20070232556A1 (en) * | 2006-03-31 | 2007-10-04 | Montine Thomas J | Methods and compositions for the treatment of neurological diseases and disorders |
BRPI0714876B1 (en) | 2006-08-04 | 2022-04-19 | Verenium Corporation | Isolated, synthetic or recombinant nucleic acid, expression cassette, cloning vector or vehicle, transformed bacterial, fungal or yeast cell, isolated, synthetic or recombinant polypeptide, composition, as well as production methods and uses thereof |
US8586006B2 (en) | 2006-08-09 | 2013-11-19 | Institute For Systems Biology | Organ-specific proteins and methods of their use |
WO2008036863A2 (en) | 2006-09-21 | 2008-03-27 | Verenium Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
CN113789316A (en) | 2006-09-21 | 2021-12-14 | 巴斯夫酶有限责任公司 | Phytases, nucleic acids encoding them and methods of making and using them |
US9845494B2 (en) | 2006-10-18 | 2017-12-19 | Affymetrix, Inc. | Enzymatic methods for genotyping on arrays |
EP2076773B1 (en) | 2006-10-24 | 2010-12-29 | Koninklijke Philips Electronics N.V. | Detecting target molecules by luminescence |
US8618248B2 (en) | 2006-10-31 | 2013-12-31 | President And Fellows Of Harvard College | Phosphopeptide compositions and anti-phosphopeptide antibody compositions and methods of detecting phosphorylated peptides |
WO2008063888A2 (en) | 2006-11-22 | 2008-05-29 | Plexxikon, Inc. | Compounds modulating c-fms and/or c-kit activity and uses therefor |
US8293684B2 (en) * | 2006-11-29 | 2012-10-23 | Exiqon | Locked nucleic acid reagents for labelling nucleic acids |
US7622295B2 (en) | 2006-12-19 | 2009-11-24 | Edelmira Cabezas | Molecular microarrays and helical peptides |
WO2008079909A1 (en) * | 2006-12-21 | 2008-07-03 | Plexxikon, Inc. | Pyrrolo [2,3-b] pyridines as kinase modulators |
WO2008080001A2 (en) | 2006-12-21 | 2008-07-03 | Plexxikon, Inc. | Compounds and methods for kinase modulation, and indications therefor |
PE20081581A1 (en) * | 2006-12-21 | 2008-11-12 | Plexxikon Inc | PIRROLO [2,3-b] PYRIDINES COMPOUNDS AS KINASE MODULATORS |
CA2673525C (en) | 2006-12-21 | 2017-12-05 | Verenium Corporation | Amylases and glucoamylases, nucleic acids encoding them and methods for making and using them |
CA2674721C (en) | 2007-01-30 | 2018-04-03 | Verenium Corporation | Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them |
KR100827449B1 (en) | 2007-02-07 | 2008-05-07 | 삼성전자주식회사 | Photolabile compound, oligomer probe array and substrate for oligomer probe array with the same and the method of fabricating the same |
JP4850854B2 (en) * | 2007-03-22 | 2012-01-11 | 信越化学工業株式会社 | Manufacturing method of substrate for producing microarray |
JP4850855B2 (en) * | 2007-03-22 | 2012-01-11 | 信越化学工業株式会社 | Manufacturing method of substrate for producing microarray |
US7572990B2 (en) * | 2007-03-30 | 2009-08-11 | Intermec Ip Corp. | Keypad overlay membrane |
EP3196310B1 (en) | 2007-04-27 | 2020-07-08 | The Regents of The University of California | Plant co2 sensors, nucleic acids encoding them, and methods for making and using them |
US8200440B2 (en) | 2007-05-18 | 2012-06-12 | Affymetrix, Inc. | System, method, and computer software product for genotype determination using probe array data |
EP2170776B1 (en) * | 2007-06-28 | 2018-11-07 | Prime Synthesis, Inc. | Method for preparing a polymer-coated controlled porosity glass particle |
US20100190777A1 (en) | 2007-07-17 | 2010-07-29 | Plexxikon Inc. | Compounds and methods for kinase modulation, and indications therefor |
US7507539B2 (en) * | 2007-07-30 | 2009-03-24 | Quest Diagnostics Investments Incorporated | Substractive single label comparative hybridization |
CA2699518A1 (en) * | 2007-09-17 | 2009-03-26 | Twof, Inc. | Supramolecular nanostamping printing device |
KR101387633B1 (en) * | 2007-10-02 | 2014-04-22 | 삼성전자 주식회사 | Binary-coupled type probe array, biochip, and fabricating method of the same |
WO2009045627A2 (en) | 2007-10-03 | 2009-04-09 | Verenium Corporation | Xylanases, nucleic acids encoding them and methods for making and using them |
JP5563990B2 (en) | 2008-01-03 | 2014-07-30 | ヴェレニウム コーポレイション | Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them |
CA2710807C (en) | 2008-03-11 | 2015-09-08 | Kyeong Man Hong | Method for measuring chromosome, gene or specific nucleotide sequence copy numbers using snp array |
EP2107125A1 (en) | 2008-03-31 | 2009-10-07 | Eppendorf Array Technologies SA (EAT) | Real-time PCR of targets on a micro-array |
WO2009126900A1 (en) | 2008-04-11 | 2009-10-15 | Pelikan Technologies, Inc. | Method and apparatus for analyte detecting device |
US20110118125A1 (en) * | 2008-05-03 | 2011-05-19 | Tufts Medical Center, Inc. | Neonatal salivary genomics |
EP2145895B1 (en) | 2008-07-08 | 2013-10-30 | Commissariat à l'Énergie Atomique et aux Énergies Alternatives | Process for the manufacturing of glycochips |
US8153391B2 (en) | 2008-08-29 | 2012-04-10 | Bunge Oils, Inc. | Hydrolases, nucleic acids encoding them and methods for making and using them |
US8198062B2 (en) | 2008-08-29 | 2012-06-12 | Dsm Ip Assets B.V. | Hydrolases, nucleic acids encoding them and methods for making and using them |
US8357503B2 (en) | 2008-08-29 | 2013-01-22 | Bunge Oils, Inc. | Hydrolases, nucleic acids encoding them and methods for making and using them |
JP5586607B2 (en) | 2008-08-29 | 2014-09-10 | ヤンセン バイオテツク,インコーポレーテツド | Markers and methods for evaluating and treating ulcerative colitis and related diseases using a 20 gene panel |
EP2352998A4 (en) | 2008-11-07 | 2011-09-21 | Centocor Ortho Biotech Inc | Markers and methods for assessing and treating lupus patients susceptible to photoprovocation |
US9375169B2 (en) | 2009-01-30 | 2016-06-28 | Sanofi-Aventis Deutschland Gmbh | Cam drive for managing disposable penetrating member actions with a single motor and motor and control system |
EP3722810A3 (en) | 2009-02-11 | 2021-01-13 | Caris MPI, Inc. | Molecular profiling of tumors |
EP2406399B1 (en) | 2009-03-09 | 2018-02-14 | Bioatla, LLC | Mirac proteins |
CR20170089A (en) * | 2009-04-03 | 2017-07-17 | Plexxikon Inc | PROPANE-ACID COMPOSITIONS-1 - SULFONIC {3- [5- (4-CHLORINE-PHENYL) -1H-PIRROLO [2,3-B] PIRIDINA-3-CARBONIL] -2,4-DIFLUORO-PHENIL} -AMIDA AND THE USE OF THE SAME |
CN102459303B (en) | 2009-05-21 | 2016-06-29 | 巴斯夫酶有限责任公司 | Phytase, the nucleic acid encoding them and preparation and their method of use |
US20100298171A1 (en) * | 2009-05-22 | 2010-11-25 | Affymetrix, Inc. | Apparatus for polymer synthesis |
US8329724B2 (en) | 2009-08-03 | 2012-12-11 | Hoffmann-La Roche Inc. | Process for the manufacture of pharmaceutically active compounds |
AU2010298000A1 (en) | 2009-09-25 | 2012-04-05 | Signature Genomics Laboratories Llc | Multiplex (+/-) stranded arrays and assays for detecting chromosomal abnormalities associated with cancer and other diseases |
UA109884C2 (en) | 2009-10-16 | 2015-10-26 | A POLYPEPTIDE THAT HAS THE ACTIVITY OF THE PHOSPHATIDYLINOSYTOL-SPECIFIC PHOSPHOLIPASE C, NUCLEIC ACID, AND METHOD OF METHOD | |
UA111708C2 (en) | 2009-10-16 | 2016-06-10 | Бандж Ойлз, Інк. | METHOD OF OIL REFINING |
WO2011048498A2 (en) | 2009-10-19 | 2011-04-28 | Stichting Het Nederlands Kanker Instituut | Differentiation between brca2-associated tumours and sporadic tumours via array comparative genomic hybridization |
WO2011048495A1 (en) | 2009-10-19 | 2011-04-28 | Stichting Het Nederlands Kanker Instituut | Predicting benefit of anti-cancer therapy via array comparative genomic hybridization |
WO2011048499A1 (en) | 2009-10-19 | 2011-04-28 | Stichting Het Nederlands Kanker Instituut | Predicting response to anti-cancer therapy via array comparative genomic hybridization |
WO2011056688A2 (en) | 2009-10-27 | 2011-05-12 | Caris Life Sciences, Inc. | Molecular profiling for personalized medicine |
NZ599866A (en) | 2009-11-06 | 2014-09-26 | Plexxikon Inc | Compounds and methods for kinase modulation, and indications therefor |
US8501122B2 (en) | 2009-12-08 | 2013-08-06 | Affymetrix, Inc. | Manufacturing and processing polymer arrays |
US20110143966A1 (en) * | 2009-12-15 | 2011-06-16 | Affymetrix, Inc. | Surface Modifications and Methods for their Synthesis and Use |
US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
DK2556171T3 (en) | 2010-04-05 | 2015-12-14 | Prognosys Biosciences Inc | Spatially CODED BIOLOGICAL ASSAYS |
US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
US8965476B2 (en) | 2010-04-16 | 2015-02-24 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
WO2012038837A2 (en) | 2010-09-20 | 2012-03-29 | Stichting Het Nederlands Kanker Instituut | Methods for predicting response to anti-cancer therapy in cancer patients |
BR112013008347A2 (en) | 2010-10-06 | 2016-06-14 | Bp Corp North America Inc | cbh variant polypeptides i |
MA34948B1 (en) | 2011-02-07 | 2014-03-01 | Plexxikon Inc | COMPOUNDS AND METHODS FOR MODULATING KINASE, AND INDICATIONS THEREOF |
TWI558702B (en) | 2011-02-21 | 2016-11-21 | 普雷辛肯公司 | Solid forms of a pharmaceutically active substance |
US8759036B2 (en) | 2011-03-21 | 2014-06-24 | Affymetrix, Inc. | Methods for synthesizing pools of probes |
GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
RU2631487C2 (en) | 2011-05-17 | 2017-09-22 | Плексксикон Инк. | Kinases modulation and indications for its use |
US8960686B2 (en) * | 2011-09-30 | 2015-02-24 | Electro Scientific Industries, Inc. | Controlled surface roughness in vacuum retention |
JP6375230B2 (en) | 2012-02-27 | 2018-08-15 | セルラー リサーチ, インコーポレイテッド | Compositions and kits for molecular counting |
ES2776673T3 (en) | 2012-02-27 | 2020-07-31 | Univ North Carolina Chapel Hill | Methods and uses for molecular tags |
US9150570B2 (en) | 2012-05-31 | 2015-10-06 | Plexxikon Inc. | Synthesis of heterocyclic compounds |
EP2722105A1 (en) * | 2012-10-22 | 2014-04-23 | Universität Wien | Method of in situ synthesizing microarrays |
KR101709424B1 (en) * | 2012-11-15 | 2017-02-22 | 제임스 앤드류 레스코세크 | Plasma gate |
US9267171B2 (en) | 2013-02-28 | 2016-02-23 | New York University | DNA photolithography with cinnamate crosslinkers |
US10535420B2 (en) | 2013-03-15 | 2020-01-14 | Affymetrix, Inc. | Systems and methods for probe design to detect the presence of simple and complex indels |
KR102126275B1 (en) | 2013-05-03 | 2020-06-25 | 삼성디스플레이 주식회사 | Apparatus for aligning a substrate and appratus for cutting a substrate using the same |
CN111662960B (en) | 2013-06-25 | 2024-04-12 | 普罗格诺西斯生物科学公司 | Spatially encoded bioanalytical analysis using microfluidic devices |
KR20230074639A (en) | 2013-08-28 | 2023-05-30 | 벡톤 디킨슨 앤드 컴퍼니 | Massively parallel single cell analysis |
KR20150078515A (en) * | 2013-12-31 | 2015-07-08 | 삼성디스플레이 주식회사 | Measuring method for reaction ratio of reactive mesogen |
US9387451B2 (en) | 2014-02-03 | 2016-07-12 | International Business Machines Corporation | Flow cell array and uses thereof |
DK3143138T3 (en) | 2014-05-13 | 2022-04-25 | Bioatla Inc | CONDITIONALLY ACTIVE BIOLOGICAL PROTEINS |
US10533216B2 (en) | 2014-05-23 | 2020-01-14 | Centrillion Technology Holdings Corporation | Oligonucleotide probe inversion process for in situ synthesized probe arrays |
US11111288B2 (en) | 2014-08-28 | 2021-09-07 | Bioatla, Inc. | Conditionally active chimeric antigen receptors for modified t-cells |
AU2015308818B2 (en) | 2014-08-28 | 2021-02-25 | Bioatla Llc | Conditionally active chimeric antigen receptors for modified T-cells |
CA2959786A1 (en) | 2014-09-03 | 2016-03-10 | Bioatla, Llc | Discovering and producing conditionally active biologic proteins in the same eukaryotic cell production hosts |
WO2016130572A2 (en) | 2015-02-10 | 2016-08-18 | Dana-Farber Cancer Institute, Inc. | Methods of determining levels of exposure to radiation and uses thereof |
CN107580603A (en) | 2015-02-24 | 2018-01-12 | 生物蛋白有限公司 | Condition active biological protein |
WO2016164641A1 (en) | 2015-04-08 | 2016-10-13 | Plexxikon Inc. | Compounds and methods for kinase modulation, and indications therefor |
DK3901281T3 (en) | 2015-04-10 | 2023-01-23 | Spatial Transcriptomics Ab | SPATIALLY SEPARATE, MULTIPLEX NUCLEIC ACID ANALYSIS OF BIOLOGICAL SAMPLES |
US10829484B2 (en) | 2015-07-28 | 2020-11-10 | Plexxikon Inc. | Compounds and methods for kinase modulation, and indications therefor |
CN106446605B (en) * | 2015-08-13 | 2021-07-20 | 生捷科技控股公司 | Method for producing polymer arrays |
US10695735B2 (en) | 2015-08-18 | 2020-06-30 | Centrillion Technology Holdings Corporation | Probe inversion process for in situ synthesized probe arrays |
KR20180071299A (en) | 2015-10-18 | 2018-06-27 | 아피메트릭스, 인코포레이티드 | Analysis of allelic genotypes of single nucleotide polymorphisms and Indel |
SG11201804711RA (en) | 2015-12-07 | 2018-07-30 | Plexxikon Inc | Compounds and methods for kinase modulation, and indications therefor |
SI3455261T1 (en) | 2016-05-13 | 2023-01-31 | Bioatla, Inc. | Anti-ror2 antibodies, antibody fragments, their immunoconjugates and uses thereof |
TW201815766A (en) | 2016-09-22 | 2018-05-01 | 美商普雷辛肯公司 | Compounds and methods for IDO and TDO modulation, and indications therefor |
AU2017395023B2 (en) | 2016-12-23 | 2022-04-07 | Plexxikon Inc. | Compounds and methods for CDK8 modulation and indications therefor |
WO2018226846A1 (en) | 2017-06-07 | 2018-12-13 | Plexxikon Inc. | Compounds and methods for kinase modulation |
US20190366292A1 (en) * | 2018-05-29 | 2019-12-05 | Centrillion Technologies, Inc. | Increasing Efficiency Of Photochemical Reactions On Substrates |
KR20210111254A (en) | 2018-11-30 | 2021-09-10 | 캐리스 엠피아이, 아이엔씨. | Next-generation molecular profiling |
US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
WO2021112918A1 (en) | 2019-12-02 | 2021-06-10 | Caris Mpi, Inc. | Pan-cancer platinum response predictor |
US11821035B1 (en) | 2020-01-29 | 2023-11-21 | 10X Genomics, Inc. | Compositions and methods of making gene expression libraries |
Family Cites Families (123)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6112373B2 (en) * | 1974-09-04 | 1986-04-08 | Hitachi Ltd | |
US4180739A (en) | 1977-12-23 | 1979-12-25 | Varian Associates, Inc. | Thermostatable flow cell for fluorescence measurements |
US4294800A (en) | 1978-10-30 | 1981-10-13 | Tavlarides Lawrence L | Liquid jet recycle reactor |
US4500707A (en) * | 1980-02-29 | 1985-02-19 | University Patents, Inc. | Nucleosides useful in the preparation of polynucleotides |
US4458066A (en) * | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
US4401796A (en) * | 1981-04-30 | 1983-08-30 | City Of Hope Research Institute | Solid-phase synthesis of polynucleotides |
US4507433A (en) * | 1983-10-07 | 1985-03-26 | The Johns Hopkins University | Preparation of oligodeoxyribonucleoside alkyl or arylphosphonates |
DE3565986D1 (en) * | 1984-05-02 | 1988-12-08 | Brendan James Hamill | An apparatus for the chemical synthesis of oligonucleotides |
ATE143289T1 (en) * | 1984-06-13 | 1996-10-15 | Applied Research Systems | APPARATUS USED IN CHEMICAL TESTING PROCEDURES |
JPS61231716A (en) * | 1985-04-08 | 1986-10-16 | Hitachi Ltd | Filming apparatus |
US4751170A (en) * | 1985-07-26 | 1988-06-14 | Nippon Telegraph And Telephone Corporation | Silylation method onto surface of polymer membrane and pattern formation process by the utilization of silylation method |
US4963245A (en) | 1986-05-02 | 1990-10-16 | Ciba Corning Diagnostics Corp. | Unitary multiple electrode sensor |
US5175209A (en) | 1987-01-06 | 1992-12-29 | Baylor College Of Medicine | Porous wafer for segmented synthesis of biopolymers |
US5525464A (en) | 1987-04-01 | 1996-06-11 | Hyseq, Inc. | Method of sequencing by hybridization of oligonucleotide probes |
US5202231A (en) | 1987-04-01 | 1993-04-13 | Drmanac Radoje T | Method of sequencing of genomes by hybridization of oligonucleotide probes |
US5100775A (en) * | 1988-03-16 | 1992-03-31 | Smyczek Peter J | Method for conducting nucleic acid hybridization in chamber with precise fluid delivery |
US5700637A (en) | 1988-05-03 | 1997-12-23 | Isis Innovation Limited | Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays |
US5188963A (en) * | 1989-11-17 | 1993-02-23 | Gene Tec Corporation | Device for processing biological specimens for analysis of nucleic acids |
GB8827853D0 (en) * | 1988-11-29 | 1988-12-29 | Ares Serono Res & Dev Ltd | Sensor for optical assay |
US5143854A (en) * | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
US5527681A (en) * | 1989-06-07 | 1996-06-18 | Affymax Technologies N.V. | Immobilized molecular synthesis of systematically substituted compounds |
US6955915B2 (en) | 1989-06-07 | 2005-10-18 | Affymetrix, Inc. | Apparatus comprising polymers |
US5547839A (en) * | 1989-06-07 | 1996-08-20 | Affymax Technologies N.V. | Sequencing of surface immobilized polymers utilizing microflourescence detection |
US5424186A (en) * | 1989-06-07 | 1995-06-13 | Affymax Technologies N.V. | Very large scale immobilized polymer synthesis |
US5242974A (en) * | 1991-11-22 | 1993-09-07 | Affymax Technologies N.V. | Polymer reversal on solid surfaces |
US5744101A (en) * | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
US5595707A (en) * | 1990-03-02 | 1997-01-21 | Ventana Medical Systems, Inc. | Automated biological reaction apparatus |
US5723289A (en) * | 1990-06-11 | 1998-03-03 | Nexstar Pharmaceuticals, Inc. | Parallel selex |
EP0562025B1 (en) * | 1990-12-06 | 2001-02-07 | Affymetrix, Inc. (a Delaware Corporation) | Compounds and their use in a binary synthesis strategy |
US5449754A (en) | 1991-08-07 | 1995-09-12 | H & N Instruments, Inc. | Generation of combinatorial libraries |
WO1993002992A1 (en) * | 1991-08-07 | 1993-02-18 | H & N Instruments, Inc. | Synthesis of chain chemical compounds |
US5187096A (en) | 1991-08-08 | 1993-02-16 | Rensselaer Polytechnic Institute | Cell substrate electrical impedance sensor with multiple electrode array |
US5639603A (en) * | 1991-09-18 | 1997-06-17 | Affymax Technologies N.V. | Synthesizing and screening molecular diversity |
US5846708A (en) * | 1991-11-19 | 1998-12-08 | Massachusetts Institiute Of Technology | Optical and electrical methods and apparatus for molecule detection |
IL103674A0 (en) * | 1991-11-19 | 1993-04-04 | Houston Advanced Res Center | Method and apparatus for molecule detection |
DE69233331T3 (en) | 1991-11-22 | 2007-08-30 | Affymetrix, Inc., Santa Clara | Combinatorial Polymersynthesis Strategies |
US5384261A (en) * | 1991-11-22 | 1995-01-24 | Affymax Technologies N.V. | Very large scale immobilized polymer synthesis using mechanically directed flow paths |
US5324633A (en) * | 1991-11-22 | 1994-06-28 | Affymax Technologies N.V. | Method and apparatus for measuring binding affinity |
US5288514A (en) * | 1992-09-14 | 1994-02-22 | The Regents Of The University Of California | Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support |
US5324483B1 (en) * | 1992-10-08 | 1996-09-24 | Warner Lambert Co | Apparatus for multiple simultaneous synthesis |
WO1994010128A1 (en) * | 1992-11-02 | 1994-05-11 | Affymax Technologies N.V. | Novel photoreactive protecting groups |
US5679548A (en) | 1993-02-02 | 1997-10-21 | The Scripps Research Institute | Methods for producing polypeptide metal binding sites and compositions thereof |
DK0826955T3 (en) | 1993-02-03 | 2002-08-19 | Histaggen Inc | Method of storing a ligand in a releasable containment agent and method of delivering a ligand therefrom |
US5368823A (en) * | 1993-02-11 | 1994-11-29 | University Of Georgia Research Foundation, Inc. | Automated synthesis of oligonucleotides |
US5534328A (en) * | 1993-12-02 | 1996-07-09 | E. I. Du Pont De Nemours And Company | Integrated chemical processing apparatus and processes for the preparation thereof |
US5837832A (en) | 1993-06-25 | 1998-11-17 | Affymetrix, Inc. | Arrays of nucleic acid probes on biological chips |
DE69431719T2 (en) * | 1993-06-25 | 2003-09-18 | Affymetrix Inc N D Ges D Staat | HYBRIDIZATION AND SEQUENCING OF NUCLEIC ACIDS |
US5472672A (en) * | 1993-10-22 | 1995-12-05 | The Board Of Trustees Of The Leland Stanford Junior University | Apparatus and method for polymer synthesis using arrays |
US6165778A (en) | 1993-11-02 | 2000-12-26 | Affymax Technologies N.V. | Reaction vessel agitation apparatus |
US5631734A (en) * | 1994-02-10 | 1997-05-20 | Affymetrix, Inc. | Method and apparatus for detection of fluorescently labeled materials |
US6015880A (en) | 1994-03-16 | 2000-01-18 | California Institute Of Technology | Method and substrate for performing multiple sequential reactions on a matrix |
DE69527585T2 (en) * | 1994-06-08 | 2003-04-03 | Affymetrix Inc | Method and device for packaging chips |
US6287850B1 (en) * | 1995-06-07 | 2001-09-11 | Affymetrix, Inc. | Bioarray chip reaction apparatus and its manufacture |
US5508197A (en) * | 1994-07-25 | 1996-04-16 | The Regents, University Of California | High-speed thermal cycling system and method of use |
US5609305A (en) * | 1994-09-19 | 1997-03-11 | Vortec Corporation | Apparatus for providing an air curtain |
US5985356A (en) | 1994-10-18 | 1999-11-16 | The Regents Of The University Of California | Combinatorial synthesis of novel materials |
US5556752A (en) * | 1994-10-24 | 1996-09-17 | Affymetrix, Inc. | Surface-bound, unimolecular, double-stranded DNA |
CA2204912C (en) | 1994-11-10 | 2005-01-04 | David Sarnoff Research Center, Inc. | Liquid distribution system |
US5659477A (en) * | 1994-12-28 | 1997-08-19 | Collins; Charles Michael | Self reproducing fundamental fabricating machines (F-Units) |
EP0729021A1 (en) | 1995-02-25 | 1996-08-28 | Roche Diagnostics GmbH | Device for treating samples on microscope slides |
US6239273B1 (en) | 1995-02-27 | 2001-05-29 | Affymetrix, Inc. | Printing molecular library arrays |
US5959098A (en) | 1996-04-17 | 1999-09-28 | Affymetrix, Inc. | Substrate preparation process |
US5599695A (en) | 1995-02-27 | 1997-02-04 | Affymetrix, Inc. | Printing molecular library arrays using deprotection agents solely in the vapor phase |
GB9506312D0 (en) * | 1995-03-28 | 1995-05-17 | Medical Res Council | Improvements in or relating to sample processing |
US5624711A (en) * | 1995-04-27 | 1997-04-29 | Affymax Technologies, N.V. | Derivatization of solid supports and methods for oligomer synthesis |
US5545531A (en) * | 1995-06-07 | 1996-08-13 | Affymax Technologies N.V. | Methods for making a device for concurrently processing multiple biological chip assays |
US6168948B1 (en) * | 1995-06-29 | 2001-01-02 | Affymetrix, Inc. | Miniaturized genetic analysis systems and methods |
US5856174A (en) * | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
US5888830A (en) * | 1995-09-22 | 1999-03-30 | Berlex Laboratories, Inc. | Apparatus and process for multiple chemical reactions |
US6132580A (en) | 1995-09-28 | 2000-10-17 | The Regents Of The University Of California | Miniature reaction chamber and devices incorporating same |
US6660233B1 (en) | 1996-01-16 | 2003-12-09 | Beckman Coulter, Inc. | Analytical biochemistry system with robotically carried bioarray |
JP3682070B2 (en) | 1996-01-23 | 2005-08-10 | ノバルティス アクチエンゲゼルシャフト | Apparatus and method for synthesizing polymer |
US6114122A (en) * | 1996-03-26 | 2000-09-05 | Affymetrix, Inc. | Fluidics station with a mounting system and method of using |
US6706875B1 (en) * | 1996-04-17 | 2004-03-16 | Affyemtrix, Inc. | Substrate preparation process |
US5958342A (en) * | 1996-05-17 | 1999-09-28 | Incyte Pharmaceuticals, Inc. | Jet droplet device |
FR2749663B1 (en) * | 1996-06-07 | 1998-07-31 | Bio Merieux | SINGLE USE ANALYSIS CARD COMPRISING A LIQUID FLOW CONDUIT |
US6057100A (en) * | 1996-06-07 | 2000-05-02 | Eos Biotechnology, Inc. | Oligonucleotide arrays |
US5981733A (en) | 1996-09-16 | 1999-11-09 | Incyte Pharmaceuticals, Inc. | Apparatus for the chemical synthesis of molecular arrays |
EP1669738A3 (en) * | 1996-10-09 | 2007-12-12 | Symyx Technologies, Inc. | Infrared spectroscopy and imaging of libraries |
US6720186B1 (en) * | 1998-04-03 | 2004-04-13 | Symyx Technologies, Inc. | Method of research for creating and testing novel catalysts, reactions and polymers |
US6001311A (en) | 1997-02-05 | 1999-12-14 | Protogene Laboratories, Inc. | Apparatus for diverse chemical synthesis using two-dimensional array |
ES2187945T3 (en) * | 1997-02-21 | 2003-06-16 | Merckle Gmbh | DEVICE AND PROCEDURE FOR AUTOMATIC SYNTHESIS OF MACROMOLECULES. |
US6126904A (en) | 1997-03-07 | 2000-10-03 | Argonaut Technologies, Inc. | Apparatus and methods for the preparation of chemical compounds |
AU751956B2 (en) * | 1997-03-20 | 2002-09-05 | University Of Washington | Solvent for biopolymer synthesis, solvent microdroplets and methods of use |
JP3270730B2 (en) * | 1997-03-21 | 2002-04-02 | 株式会社日立国際電気 | Substrate processing apparatus and substrate processing method |
US6420108B2 (en) * | 1998-02-09 | 2002-07-16 | Affymetrix, Inc. | Computer-aided display for comparative gene expression |
US5814365A (en) * | 1997-08-15 | 1998-09-29 | Micro C Technologies, Inc. | Reactor and method of processing a semiconductor substate |
JP3498201B2 (en) | 1997-08-27 | 2004-02-16 | アークレイ株式会社 | Vacuum generator and sample analyzer using the same |
US6150147A (en) | 1998-02-06 | 2000-11-21 | Affymetrix, Inc. | Biological array fabrication methods with reduction of static charge |
US6165069A (en) | 1998-03-11 | 2000-12-26 | Digideal Corporation | Automated system for playing live casino table games having tabletop changeable playing card displays and monitoring security features |
US6251595B1 (en) * | 1998-06-18 | 2001-06-26 | Agilent Technologies, Inc. | Methods and devices for carrying out chemical reactions |
EP0977240A1 (en) | 1998-07-30 | 2000-02-02 | IMEC vzw | System, method and apparatus for processing semiconductors |
US6461812B2 (en) * | 1998-09-09 | 2002-10-08 | Agilent Technologies, Inc. | Method and multiple reservoir apparatus for fabrication of biomolecular arrays |
DE19846749C2 (en) * | 1998-10-12 | 2000-10-12 | Junker Gmbh O | Aerodynamic sealing of continuous heat treatment plants with a protective gas atmosphere |
CA2255850C (en) * | 1998-12-07 | 2000-10-17 | Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food | Rotary thermocycling apparatus |
CA2294572A1 (en) | 1999-01-27 | 2000-07-27 | Affymetrix, Inc. | Genetic compositions and methods |
US6749814B1 (en) * | 1999-03-03 | 2004-06-15 | Symyx Technologies, Inc. | Chemical processing microsystems comprising parallel flow microreactors and methods for using same |
US6242266B1 (en) * | 1999-04-30 | 2001-06-05 | Agilent Technologies Inc. | Preparation of biopolymer arrays |
US6323043B1 (en) | 1999-04-30 | 2001-11-27 | Agilent Technologies, Inc. | Fabricating biopolymer arrays |
US6258593B1 (en) * | 1999-06-30 | 2001-07-10 | Agilent Technologies Inc. | Apparatus for conducting chemical or biochemical reactions on a solid surface within an enclosed chamber |
US6306599B1 (en) | 1999-07-16 | 2001-10-23 | Agilent Technologies Inc. | Biopolymer arrays and their fabrication |
US6180351B1 (en) * | 1999-07-22 | 2001-01-30 | Agilent Technologies Inc. | Chemical array fabrication with identifier |
US6232072B1 (en) * | 1999-10-15 | 2001-05-15 | Agilent Technologies, Inc. | Biopolymer array inspection |
US6440669B1 (en) * | 1999-11-10 | 2002-08-27 | Agilent Technologies, Inc. | Methods for applying small volumes of reagents |
NO310385B1 (en) * | 1999-11-17 | 2001-06-25 | Torstein Ljungmann | Device for automatic adhesion of cover glass to slides |
JP3723712B2 (en) * | 2000-02-10 | 2005-12-07 | 株式会社日立国際電気 | Substrate processing apparatus and substrate processing method |
JP2001267991A (en) | 2000-03-16 | 2001-09-28 | Sony Corp | Transmitter, transmission method, communication system and communication method |
US6599693B1 (en) * | 2000-07-31 | 2003-07-29 | Agilent Technologies Inc. | Array fabrication |
US6613893B1 (en) | 2000-07-31 | 2003-09-02 | Agilent Technologies Inc. | Array fabrication |
US6656740B1 (en) | 2000-10-31 | 2003-12-02 | Agilent Technologies, Inc. | Pressure variation in array fabrication |
US6484104B2 (en) * | 2001-02-15 | 2002-11-19 | Klaus Abraham-Fuchs | Network for evaluating data obtained in a biochip measurement device |
US6867050B2 (en) * | 2001-05-16 | 2005-03-15 | Academia Sinica | Apparatus and methods for chemical synthesis |
US6713023B2 (en) | 2001-06-29 | 2004-03-30 | Agilent Technologies, Inc. | Flow cell for chemical reactions |
US6649348B2 (en) * | 2001-06-29 | 2003-11-18 | Agilent Technologies Inc. | Methods for manufacturing arrays |
US6696298B2 (en) * | 2001-12-07 | 2004-02-24 | Biosearch Technologies, Inc. | Multi-channel reagent dispensing apparatus |
US6935727B2 (en) * | 2001-12-18 | 2005-08-30 | Agilent Technologies, Inc. | Pulse jet print head assembly having multiple reservoirs and methods for use in the manufacture of biopolymeric arrays |
US6790620B2 (en) * | 2001-12-24 | 2004-09-14 | Agilent Technologies, Inc. | Small volume chambers |
US7141368B2 (en) * | 2002-01-30 | 2006-11-28 | Agilent Technologies, Inc. | Multi-directional deposition in array fabrication |
US20030143329A1 (en) * | 2002-01-30 | 2003-07-31 | Shchegrova Svetlana V. | Error correction in array fabrication |
EP1573056A4 (en) * | 2002-05-17 | 2007-11-28 | Nugen Technologies Inc | Methods for fragmentation, labeling and immobilization of nucleic acids |
US20030232140A1 (en) | 2002-06-14 | 2003-12-18 | Joseph Remick | Methods for reagent removal in flow chambers |
US7332273B2 (en) | 2002-06-20 | 2008-02-19 | Affymetrix, Inc. | Antireflective coatings for high-resolution photolithographic synthesis of DNA arrays |
US20040009608A1 (en) * | 2002-07-10 | 2004-01-15 | Caren Michael P. | Arrays with positioning control |
-
1996
- 1996-04-17 US US08/634,053 patent/US5959098A/en not_active Expired - Lifetime
-
1997
- 1997-04-16 EP EP97922350A patent/EP0950112A4/en not_active Withdrawn
- 1997-04-16 JP JP9537413A patent/JP2000508542A/en active Pending
- 1997-04-16 AU AU28047/97A patent/AU2804797A/en not_active Abandoned
- 1997-04-16 WO PCT/US1997/006535 patent/WO1997039151A1/en active Application Filing
- 1997-04-16 CA CA2251755A patent/CA2251755C/en not_active Expired - Lifetime
-
1999
- 1999-02-04 US US09/244,568 patent/US6307042B1/en not_active Expired - Lifetime
-
2006
- 2006-02-22 JP JP2006044640A patent/JP2006258806A/en active Pending
- 2006-08-08 US US11/500,411 patent/US20090062148A1/en not_active Abandoned
-
2009
- 2009-12-09 US US12/634,475 patent/US8067578B2/en not_active Expired - Fee Related
-
2011
- 2011-10-14 US US13/273,399 patent/US8309496B2/en not_active Expired - Fee Related
-
2012
- 2012-09-28 US US13/629,861 patent/US8637434B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
AU2804797A (en) | 1997-11-07 |
EP0950112A1 (en) | 1999-10-20 |
US5959098A (en) | 1999-09-28 |
US8309496B2 (en) | 2012-11-13 |
JP2000508542A (en) | 2000-07-11 |
US20100331217A1 (en) | 2010-12-30 |
US20090062148A1 (en) | 2009-03-05 |
CA2251755C (en) | 2010-09-14 |
US8067578B2 (en) | 2011-11-29 |
US20130059763A1 (en) | 2013-03-07 |
EP0950112A4 (en) | 2004-12-15 |
US8637434B2 (en) | 2014-01-28 |
US20120071361A1 (en) | 2012-03-22 |
JP2006258806A (en) | 2006-09-28 |
US6307042B1 (en) | 2001-10-23 |
WO1997039151A1 (en) | 1997-10-23 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CA2251755A1 (en) | Photolabile polymer array synthesis methods | |
US6887665B2 (en) | Methods of array synthesis | |
EP0946286B1 (en) | Chemical amplification for the synthesis of patterned arrays | |
EP1956028B1 (en) | Photolabile compound, oligomer probe array and substrate for oligomer probe array containing the same, and manufacturing method of the same | |
US6300137B1 (en) | Method for synthesizing a specific, surface-bound polymer uniformly over an element of a molecular array | |
US5658734A (en) | Process for synthesizing chemical compounds | |
CN101473220B (en) | Electrode array device having an adsorbed porous reaction layer | |
US7901871B2 (en) | Composition containing a photoacid generator monomer, substrate coated with the composition, method for synthesizing a compound on a substrate using the composition, and microarray produced according to the method | |
US5556961A (en) | Nucleosides with 5'-O-photolabile protecting groups | |
JP2004144768A5 (en) | ||
CN101156062B (en) | Electrode array device having an adsorbed porous reaction layer having a linker moiety | |
WO1994010128A1 (en) | Novel photoreactive protecting groups | |
CN101221121B (en) | Method of fabricating microarray | |
US20050101765A1 (en) | Photocleavable protecting groups | |
US20110201528A1 (en) | Methods Of Forming An Oligomer Array | |
McGall et al. | Photolithographic synthesis of high-density oligonucleotide arrays | |
KR100791335B1 (en) | Microarray and method of fabricating the same | |
US6184347B1 (en) | Minimization of blooming in high-density arrays by using reactive wash reagents | |
Xiao et al. | DNA microarray synthesis by using PDMS molecular stamp (II) Oligonucleotide on-chip synthesis using PDMS stamp | |
US20100056397A1 (en) | Method of Producing Microarray Having Immobilized Double-Stranded Nucleic Acid Probe Including Double-Stranded Region and Single-Stranded Region | |
KR20110111720A (en) | Method of fabricating microarray | |
EP1958690A1 (en) | Substrate structure, oligomer probe array and methods for producing the same | |
KR100801080B1 (en) | Photolabile compound and substrate for oligomer probe array with the same | |
CN110922441B (en) | Linking matrix for light-oriented microarray DNA synthesis and synthesis method | |
EP1979088A2 (en) | Use of acid scavengers for the synthesis of standard length and long-mer nucleic acid arrays |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKEX | Expiry |
Effective date: 20170418 |