CA2262018A1 - Nanoparticles having oligonucleotides attached thereto and uses therefor - Google Patents

Nanoparticles having oligonucleotides attached thereto and uses therefor

Info

Publication number
CA2262018A1
CA2262018A1 CA002262018A CA2262018A CA2262018A1 CA 2262018 A1 CA2262018 A1 CA 2262018A1 CA 002262018 A CA002262018 A CA 002262018A CA 2262018 A CA2262018 A CA 2262018A CA 2262018 A1 CA2262018 A1 CA 2262018A1
Authority
CA
Canada
Prior art keywords
nanoparticles
nucleic acid
oligonucleotides
provides
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002262018A
Other languages
French (fr)
Other versions
CA2262018C (en
Inventor
Chad A. Mirkin
Robert L. Letsinger
Robert C. Mucic
James J. Storhoff
Robert Elghanian
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanosphere LLC
Original Assignee
Nanosphere Llc
Chad A. Mirkin
Robert L. Letsinger
Robert C. Mucic
James J. Storhoff
Robert Elghanian
Northwestern University
Nanosphere, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanosphere Llc, Chad A. Mirkin, Robert L. Letsinger, Robert C. Mucic, James J. Storhoff, Robert Elghanian, Northwestern University, Nanosphere, Inc. filed Critical Nanosphere Llc
Publication of CA2262018A1 publication Critical patent/CA2262018A1/en
Application granted granted Critical
Publication of CA2262018C publication Critical patent/CA2262018C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/005—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00646—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports
    • B01J2219/00648—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports by the use of solid beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00653—Making arrays on substantially continuous surfaces the compounds being bound to electrodes embedded in or on the solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82B—NANOSTRUCTURES FORMED BY MANIPULATION OF INDIVIDUAL ATOMS, MOLECULES, OR LIMITED COLLECTIONS OF ATOMS OR MOLECULES AS DISCRETE UNITS; MANUFACTURE OR TREATMENT THEREOF
    • B82B1/00—Nanostructures formed by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/924—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis using nanostructure as support of dna analysis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Nanotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Composite Materials (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • General Physics & Mathematics (AREA)
  • Materials Engineering (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)

Abstract

The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing the nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
CA002262018A 1996-07-29 1997-07-21 Nanoparticles having oligonucleotides attached thereto and uses therefor Expired - Lifetime CA2262018C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US3180996P 1996-07-29 1996-07-29
US60/031,809 1996-07-29
PCT/US1997/012783 WO1998004740A1 (en) 1996-07-29 1997-07-21 Nanoparticles having oligonucleotides attached thereto and uses therefor

Publications (2)

Publication Number Publication Date
CA2262018A1 true CA2262018A1 (en) 1998-02-05
CA2262018C CA2262018C (en) 2007-10-02

Family

ID=21861513

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002262018A Expired - Lifetime CA2262018C (en) 1996-07-29 1997-07-21 Nanoparticles having oligonucleotides attached thereto and uses therefor

Country Status (8)

Country Link
US (1) US6773884B2 (en)
EP (2) EP1818417B1 (en)
JP (4) JP4245664B2 (en)
AU (1) AU4043497A (en)
CA (1) CA2262018C (en)
DE (1) DE69737598T2 (en)
ES (1) ES2287956T3 (en)
WO (1) WO1998004740A1 (en)

Families Citing this family (233)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7098320B1 (en) 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6361944B1 (en) 1996-07-29 2002-03-26 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6984491B2 (en) * 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
CA2262018C (en) 1996-07-29 2007-10-02 Nanosphere Llc Nanoparticles having oligonucleotides attached thereto and uses therefor
AU2004205147B2 (en) * 1996-07-29 2008-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
US7169556B2 (en) * 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6582921B2 (en) 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
US6750016B2 (en) 1996-07-29 2004-06-15 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6506564B1 (en) 1996-07-29 2003-01-14 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US5939021A (en) * 1997-01-23 1999-08-17 Hansen; W. Peter Homogeneous binding assay
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US20050037397A1 (en) * 2001-03-28 2005-02-17 Nanosphere, Inc. Bio-barcode based detection of target analytes
US6607829B1 (en) 1997-11-13 2003-08-19 Massachusetts Institute Of Technology Tellurium-containing nanocrystalline materials
US6322901B1 (en) 1997-11-13 2001-11-27 Massachusetts Institute Of Technology Highly luminescent color-selective nano-crystalline materials
US6699723B1 (en) 1997-11-25 2004-03-02 The Regents Of The University Of California Organo luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes
US5990479A (en) 1997-11-25 1999-11-23 Regents Of The University Of California Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes
US6207392B1 (en) 1997-11-25 2001-03-27 The Regents Of The University Of California Semiconductor nanocrystal probes for biological applications and process for making and using such probes
US6261783B1 (en) 1997-12-15 2001-07-17 Gilead Sciences, Inc. Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
US5989823A (en) 1998-09-18 1999-11-23 Nexstar Pharmaceuticals, Inc. Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
US6686150B1 (en) 1998-01-27 2004-02-03 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection
US6428811B1 (en) 1998-03-11 2002-08-06 Wm. Marsh Rice University Temperature-sensitive polymer/nanoshell composites for photothermally modulated drug delivery
US6699724B1 (en) 1998-03-11 2004-03-02 Wm. Marsh Rice University Metal nanoshells for biosensing applications
US7955561B2 (en) 1998-06-09 2011-06-07 The California Institute Of Technology Colloidal particles used in sensing array
EP1084390A4 (en) * 1998-06-09 2005-07-27 California Inst Of Techn Colloidal particles used in sensing arrays
US6290839B1 (en) 1998-06-23 2001-09-18 Clinical Micro Sensors, Inc. Systems for electrophoretic transport and detection of analytes
US6761816B1 (en) 1998-06-23 2004-07-13 Clinical Micro Systems, Inc. Printed circuit boards with monolayers and capture ligands
US6770479B1 (en) * 1998-07-10 2004-08-03 The United States Of America As Represented By The Secretary Of The Army Anthrax vaccine
EP0990903B1 (en) * 1998-09-18 2003-03-12 Massachusetts Institute Of Technology Biological applications of semiconductor nanocrystals
US6326144B1 (en) 1998-09-18 2001-12-04 Massachusetts Institute Of Technology Biological applications of quantum dots
US6251303B1 (en) 1998-09-18 2001-06-26 Massachusetts Institute Of Technology Water-soluble fluorescent nanocrystals
US6617583B1 (en) 1998-09-18 2003-09-09 Massachusetts Institute Of Technology Inventory control
EP1116036B1 (en) * 1998-09-18 2004-08-11 Massachusetts Institute Of Technology Water-soluble fluorescent semiconductor nanocrystals
US6306610B1 (en) 1998-09-18 2001-10-23 Massachusetts Institute Of Technology Biological applications of quantum dots
ATE389030T1 (en) 1998-09-24 2008-03-15 Univ Indiana Res & Tech Corp WATER-SOLUBLE LUMINESCENT QUANTUM-DOTS AND THEIR BIOCONJUGATES
WO2000024941A1 (en) 1998-10-27 2000-05-04 Clinical Micro Sensors, Inc. Detection of target analytes using particles and electrodes
US6261779B1 (en) * 1998-11-10 2001-07-17 Bio-Pixels Ltd. Nanocrystals having polynucleotide strands and their use to form dendrimers in a signal amplification system
EP1141762A1 (en) * 1998-12-17 2001-10-10 Leica Microsystems Heidelberg GmbH Method for differentiated investigation of diverse structures in preferably biological preparations
WO2000043783A2 (en) * 1999-01-23 2000-07-27 Minerva Biotechnologies Corporation Assays involving colloids and non-colloidal structures
EP1039291A1 (en) 1999-03-26 2000-09-27 Sony International (Europe) GmbH Optochemical sensor and method for its construction
CN1311005C (en) 1999-04-09 2007-04-18 迪纳尔生物技术公司 Process for the preparation of monodisperse polymer particles
ATE439452T1 (en) 1999-05-07 2009-08-15 Life Technologies Corp METHOD FOR DETECTING ANALYTES USING SEMICONDUCTOR NANOCRYSTALS
US20030096321A1 (en) * 1999-05-19 2003-05-22 Jose Remacle Method for the identification and/or the quantification of a target compound obtained from a biological sample upon chips
DE60000583T3 (en) 1999-05-19 2009-04-30 Eppendorf Array Technologies METHOD FOR IDENTIFYING AND / OR QUANTIFYING A TARGET LINK
IL130326A0 (en) * 1999-06-07 2000-06-01 Yeda Res & Dev A sensor based on molecular controlled semiconductor resistor
CA2376623C (en) * 1999-06-25 2011-04-19 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
JP2003504642A (en) * 1999-07-16 2003-02-04 ダブリューエム・マーシュ・ライス・ユニバーシティー Metallic fine shell for biosensing applications
WO2001007665A2 (en) 1999-07-26 2001-02-01 Clinical Micro Sensors, Inc. Sequence determination of nucleic acids using electronic detection
CA2381568A1 (en) 1999-07-30 2001-02-08 The Penn State Research Foundation Instruments, methods and reagents for surface plasmon resonance
US8497131B2 (en) 1999-10-06 2013-07-30 Becton, Dickinson And Company Surface enhanced spectroscopy-active composite nanoparticles comprising Raman-active reporter molecules
US7192778B2 (en) 1999-10-06 2007-03-20 Natan Michael J Surface enhanced spectroscopy-active composite nanoparticles
US6875619B2 (en) 1999-11-12 2005-04-05 Motorola, Inc. Microfluidic devices comprising biochannels
BR0016621A (en) * 1999-12-21 2002-09-03 Johnson & Johnson Nucleic acid detection
AU774593C (en) 2000-01-13 2005-06-23 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US20020004246A1 (en) * 2000-02-07 2002-01-10 Daniels Robert H. Immunochromatographic methods for detecting an analyte in a sample which employ semiconductor nanocrystals as detectable labels
WO2001061040A1 (en) * 2000-02-16 2001-08-23 Quantum Dot Corporation Microarray methods utilizing semiconductor nanocrystals
US6761877B2 (en) * 2000-02-18 2004-07-13 Biocrystal, Ltd. Functionalized encapsulated fluorescent nanocrystals
US7129222B2 (en) 2000-03-10 2006-10-31 Dynavax Technologies Corporation Immunomodulatory formulations and methods for use thereof
US20030129251A1 (en) 2000-03-10 2003-07-10 Gary Van Nest Biodegradable immunomodulatory formulations and methods for use thereof
WO2001071867A2 (en) 2000-03-14 2001-09-27 Massachusetts Institute Of Technology Optical amplifiers and lasers
AU4116401A (en) 2000-03-16 2001-09-24 Matsushita Electric Industrial Co., Ltd. Nucleotide detector, process for producing the same and process for forming fineparticle membrane
EP1266223B1 (en) 2000-03-20 2007-05-16 Massachusetts Institute of Technology Inorganic particle conjugates
ATE487136T1 (en) * 2000-03-28 2010-11-15 Nanosphere Inc NANOPARTICLES WITH BONDED OLIGONUCLEOTIDES AND USES THEREOF
US20030153006A1 (en) * 2000-05-22 2003-08-14 Masao Washizu Novel method for forming polymer pattern
US6706474B1 (en) 2000-06-27 2004-03-16 Board Of Trustees Of The University Of Illinois Nucleic acid enzyme biosensors for ions
DE50108396D1 (en) * 2000-07-01 2006-01-19 Clondiag Chip Tech Gmbh PROCESS FOR THE QUALITATIVE AND / OR QUANTITATIVE DETECTION OF MOLECULAR INTERACTIONS ON PROBE ARRAYS
AU7687001A (en) 2000-07-11 2002-01-21 Nanospherre Inc Method of detection by enhancement of silver staining
AU2001281248A1 (en) * 2000-08-11 2002-03-13 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
ATE349011T1 (en) 2000-10-03 2007-01-15 Minerva Biotechnologies Corp MAGNETIC IN SITU DILUTION PROCESS
IL138988A (en) * 2000-10-12 2005-09-25 Yissum Res Dev Co Dendritically amplified detection method
US7887885B2 (en) 2000-10-20 2011-02-15 Northwestern University Nanolithography methods and products therefor and produced thereby
EP1348034B1 (en) * 2000-11-15 2016-07-20 Minerva Biotechnologies Corporation Oligonucleotide identifiers
WO2002046483A2 (en) * 2000-12-06 2002-06-13 Northwestern University Silver stain removal from dna detection chips by cyanide etching or sonication
EP1356109A2 (en) * 2000-12-08 2003-10-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
DE10162176B4 (en) * 2000-12-13 2006-03-23 Institut für Physikalische Hochtechnologie e.V. Method for the simultaneous position-specific cutting of DNA
US6861263B2 (en) 2001-01-26 2005-03-01 Surromed, Inc. Surface-enhanced spectroscopy-active sandwich nanoparticles
US20020110180A1 (en) 2001-02-09 2002-08-15 Barney Alfred A. Temperature-sensing composition
US20060040286A1 (en) * 2001-03-28 2006-02-23 Nanosphere, Inc. Bio-barcode based detection of target analytes
WO2002079490A2 (en) * 2001-03-28 2002-10-10 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified particles
US20030113740A1 (en) * 2001-04-26 2003-06-19 Mirkin Chad A. Oligonucleotide-modified ROMP polymers and co-polymers
US7238472B2 (en) * 2001-05-25 2007-07-03 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7147687B2 (en) * 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
AU2002320058A1 (en) * 2001-06-06 2002-12-16 The General Hspital Corporation Magnetic-nanoparticle conjugates and methods of use
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
US7517496B2 (en) 2001-07-17 2009-04-14 Bio-Rad Laboratories, Inc. Latex based adsorbent chip
EP1483794A4 (en) * 2001-07-17 2006-05-03 Ciphergen Biosystems Inc Latex based adsorbent chip
AU2002365115A1 (en) 2001-07-20 2003-09-02 North Carolina State University Light addressable electrochemical detection of duplex structures
FR2829580B1 (en) 2001-09-07 2004-02-13 Bio Merieux HYBRID OR COMPLEX READING, DETECTION OR QUANTIFICATION METHOD USED IN THIS METHOD AND BIOPUCE USING THE SAME
JP4383865B2 (en) 2001-09-17 2009-12-16 マサチューセッツ・インスティテュート・オブ・テクノロジー Semiconductor nanocrystal composite
DE60228128D1 (en) 2001-11-09 2008-09-18 Nanosphere Inc Bioconjugate NANOPARTICLE PROBES
US7361310B1 (en) 2001-11-30 2008-04-22 Northwestern University Direct write nanolithographic deposition of nucleic acids from nanoscopic tips
US20030186245A1 (en) * 2002-03-28 2003-10-02 Roitman Daniel B. Biomolecular sensors and detection methods utilizing photoinduced charge separation
US20040009506A1 (en) * 2002-03-29 2004-01-15 Genentech, Inc. Methods and compositions for detection and quantitation of nucleic acid analytes
AU2003253590A1 (en) * 2002-03-29 2003-11-10 Board Of Regents For The Oklahoma Agricultural And Mechanical Colleges, Acting For And On Behalf Of Oklahoma State University Implantable biosensor from stratified nanostructured membranes
US20030211488A1 (en) 2002-05-07 2003-11-13 Northwestern University Nanoparticle probs with Raman spectrocopic fingerprints for analyte detection
US6890719B2 (en) 2002-05-10 2005-05-10 The Board Of Trustess Of The University Of Illinois Fluorescence based biosensor
US7534560B2 (en) 2002-05-10 2009-05-19 The Board Of Trustees Of The University Of Illinois Simple catalytic DNA biosensors for ions based on color changes
JP2006501486A (en) * 2002-05-14 2006-01-12 ナノスフェアー　インコーポレイテッド Electrical detection of DNA hybridization and specific binding events
EP1532274A4 (en) * 2002-06-28 2006-09-27 Purdue Research Foundation Magnetic nanomaterials and methods for detection of biological materials
JP2005532456A (en) * 2002-07-02 2005-10-27 ナノスフェアー　インコーポレイテッド Nanoparticle polyanion complex and its use in the detection of analytes
US7319709B2 (en) 2002-07-23 2008-01-15 Massachusetts Institute Of Technology Creating photon atoms
DE60211685T2 (en) * 2002-08-02 2007-05-10 Sony Deutschland Gmbh Process for binding hydrophilic substances to hydrophilic macromolecules and immobilizing them on hydrophobic surfaces
US8020433B2 (en) * 2003-03-25 2011-09-20 Tearlab Research, Inc. Systems and methods for a sample fluid collection device
US7810380B2 (en) 2003-03-25 2010-10-12 Tearlab Research, Inc. Systems and methods for collecting tear film and measuring tear film osmolarity
EP1537263B1 (en) 2002-08-13 2010-11-17 Massachusetts Institute Of Technology Semiconductor nanocrystal heterostructures
JP2006502232A (en) 2002-08-15 2006-01-19 モウンギ ジー． バウエンディ Stabilized semiconductor nanocrystals
US7361821B2 (en) 2002-09-20 2008-04-22 Intel Corporation Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading
WO2004038037A2 (en) 2002-09-20 2004-05-06 Intel Corporation Controlled alignment of nano-barcodes encoding specific information for scanning probe microscopy (spm) reading
WO2005016115A2 (en) 2003-01-23 2005-02-24 Montana State University Biosensors utilizing dendrimer-immobilized ligands and their use thereof
EP1597399A2 (en) * 2003-02-27 2005-11-23 Nanosphere, Inc. Label-free gene expression profiling with universal nanoparticle probes in microarray assay format
US7181266B2 (en) 2003-03-04 2007-02-20 Massachusetts Institute Of Technology Materials and methods for near-infrared and infrared lymph node mapping
US7612185B2 (en) 2003-03-07 2009-11-03 The Board Of Trustees Of The University Of Illinois Nucleic acid biosensors
DE602004011198T2 (en) * 2003-03-25 2008-12-24 Biacore Ab IMMOBILIZATION PROCESS AND KIT THEREFOR
DE10315074A1 (en) * 2003-04-02 2004-10-14 Clondiag Chip Technologies Gmbh Device for the duplication and detection of nucleic acids
US7917298B1 (en) 2003-04-17 2011-03-29 Nanosys, Inc. Nanocrystal taggants
WO2004111602A2 (en) * 2003-05-16 2004-12-23 University Of Rochester Colorimetric and fluorescent methods for sensing of oligonucleotides
US20050250094A1 (en) * 2003-05-30 2005-11-10 Nanosphere, Inc. Method for detecting analytes based on evanescent illumination and scatter-based detection of nanoparticle probe complexes
JP2007525970A (en) 2003-06-27 2007-09-13 ナノスフェアー　インコーポレイテッド Target analyte detection based on bio barcode
US7229497B2 (en) 2003-08-26 2007-06-12 Massachusetts Institute Of Technology Method of preparing nanocrystals
US7354706B2 (en) * 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
US20090163375A1 (en) 2003-09-09 2009-06-25 Bowman Christopher N Use of Photopolymerization for Amplification and Detection of a Molecular Recognition Event
US7279280B2 (en) * 2003-09-25 2007-10-09 Mgp Biotech, Inc. Apparatus and method for detecting genetic mutations and single nucleotide polymorphisms
FR2860872A1 (en) 2003-10-09 2005-04-15 Commissariat Energie Atomique MICRO-SENSORS AND NANO-SENSORS OF CHEMICAL AND BIOLOGICAL SPECIES WITH SURFACE PLASMONS
JP2007516843A (en) * 2003-10-20 2007-06-28 ザ・レジェンツ・オブ・ザ・ユニバーシティ・オブ・カリフォルニア Nanoscale conversion system for detecting molecular interactions
US20100000881A1 (en) * 2003-10-30 2010-01-07 North Carolina State University Electrochemical detection of nucleic acid hybridization
JP4732683B2 (en) * 2003-12-29 2011-07-27 ユニバーサル・バイオ・リサーチ株式会社 Target substance detection method
US7485419B2 (en) 2004-01-13 2009-02-03 The Board Of Trustees Of The University Of Illinois Biosensors based on directed assembly of particles
CA2565166C (en) * 2004-05-12 2017-12-12 Nanosphere, Inc. Bio-barcode based detection of target analytes
GB0411537D0 (en) * 2004-05-24 2004-06-23 Midatech Ltd Nanoparticles comprising rna ligands
JP5398982B2 (en) * 2004-05-24 2014-01-29 ミダテック リミテッド Nanoparticles containing RNA ligands
US20080286880A1 (en) * 2004-07-07 2008-11-20 The Penn State Research Foundation Methods and Systems for Nanoparticle Enhancement of Signals
US7229690B2 (en) 2004-07-26 2007-06-12 Massachusetts Institute Of Technology Microspheres including nanoparticles
CA2572178A1 (en) * 2004-07-26 2006-03-16 Nanosphere, Inc. Method for distinguishing methicillin resistant s. aureus from methicillin sensitive s. aureus in a mixed culture
WO2006033732A1 (en) 2004-08-17 2006-03-30 Invitrogen Corporation Synthesis of highly luminescent colloidal particles
US9494581B2 (en) 2004-08-24 2016-11-15 University Of Wyoming System and method for Raman spectroscopy assay using paramagnetic particles
US7316967B2 (en) 2004-09-24 2008-01-08 Massachusetts Institute Of Technology Flow method and reactor for manufacturing noncrystals
DE602005019791D1 (en) 2004-11-16 2010-04-15 Illumina Inc METHOD AND DEVICE FOR READING CODED MICROBALLS
US7485471B1 (en) * 2004-12-17 2009-02-03 Intel Corporation Detection of enhanced multiplex signals by surface enhanced Raman spectroscopy
US8134175B2 (en) 2005-01-11 2012-03-13 Massachusetts Institute Of Technology Nanocrystals including III-V semiconductors
JP2008527999A (en) * 2005-01-21 2008-07-31 ユニバーシティー オブ ロチェスター Methods for separating short single stranded nucleic acids from long single stranded nucleic acids and double stranded nucleic acids, and related biomolecular assays
JP2006247492A (en) * 2005-03-09 2006-09-21 Ebara Corp Solid-phase synthesis apparatus
WO2006104979A2 (en) * 2005-03-29 2006-10-05 Nanosphere, Inc. Method for detecting a target analyte
CA2606924C (en) 2005-05-09 2013-04-16 The General Hospital Corporation Water relaxation-based sensors
WO2006125124A2 (en) * 2005-05-18 2006-11-23 Nanosphere, Inc. Substrate functionalization method for high sensitivity applications
US8067393B2 (en) 2005-05-26 2011-11-29 Japan Science And Technology Agency Sugar-immobilized metal nanoparticle, method for measuring sugar-protein interaction using the same and method for recovering protein from sugar-protein interactant
US8252756B2 (en) 2005-06-14 2012-08-28 Northwestern University Nucleic acid functionalized nanoparticles for therapeutic applications
WO2007002567A2 (en) * 2005-06-23 2007-01-04 Nanosphere, Inc. Selective isolation and concentration of nucleic acids from complex samples
JP4822753B2 (en) * 2005-07-11 2011-11-24 一般社団法人オンチップ・セロミクス・コンソーシアム Cell component sorting chip, cell component analysis system, and cell component analysis method using them
JP2009501539A (en) * 2005-07-21 2009-01-22 テティス・エッセエッレエッレ Support with nanostructured titanium dioxide membrane and use thereof
US20110229579A1 (en) * 2005-07-21 2011-09-22 Roberta Carbone Support Having Nanostructured Titanium Dioxide Film And Uses Thereof
EP1940734A2 (en) 2005-08-10 2008-07-09 Northwestern University Composite particles
US7892734B2 (en) 2005-08-11 2011-02-22 The Board Of Trustees Of The University Of Illinois Aptamer based colorimetric sensor systems
ATE489482T1 (en) * 2005-08-19 2010-12-15 Nanosphere Inc METHOD FOR PRODUCING HYBRID SUBSTRATES WITH DNA AND ANTIBODIES AND USES THEREOF
JP5295769B2 (en) 2005-08-31 2013-09-18 ティツー・バイオシステムズ・インコーポレーテッド NMR apparatus for detecting analytes
US8409863B2 (en) 2005-12-14 2013-04-02 Becton, Dickinson And Company Nanoparticulate chemical sensors using SERS
JP5537034B2 (en) 2005-12-23 2014-07-02 ナノストリング テクノロジーズ，インコーポレーテッド Nanoreporter and method for producing and using the same
US7394094B2 (en) 2005-12-29 2008-07-01 Massachusetts Institute Of Technology Semiconductor nanocrystal heterostructures
US7723100B2 (en) 2006-01-13 2010-05-25 Becton, Dickinson And Company Polymer coated SERS nanotag
EP1981994B1 (en) * 2006-02-08 2014-06-04 Becton, Dickinson and Company Sers nanotag assays
US7799554B2 (en) 2006-03-16 2010-09-21 The Board Of Trustees Of The University Of Illinois Lateral flow devices
US7830575B2 (en) 2006-04-10 2010-11-09 Illumina, Inc. Optical scanner with improved scan time
US7964347B2 (en) * 2006-06-15 2011-06-21 Krassen Dimitrov Labels for electronic detection of individual molecules and methods for their detection
WO2008097328A2 (en) * 2006-06-23 2008-08-14 Northwestern University Asymmetric functionalized nanoparticles and methods of use
JPWO2008035565A1 (en) * 2006-09-19 2010-01-28 コニカミノルタエムジー株式会社 Biomolecule detection reagent and biomolecule detection method using the same
US7847238B2 (en) 2006-11-07 2010-12-07 New York University Holographic microfabrication and characterization system for soft matter and biological systems
AU2007317787B2 (en) 2006-11-08 2012-07-12 T2 Biosystems, Inc. NMR systems for in vivo detection of analytes
WO2008076414A2 (en) 2006-12-15 2008-06-26 New York University Self-replicating materials
JP2008154493A (en) 2006-12-22 2008-07-10 Rohm Co Ltd Separation/purification method and microfluid circuit
WO2008089248A2 (en) 2007-01-19 2008-07-24 The Board Of Trustees Of The University Of Illinois Amphiphilic substances and triggered liberation from lipid vesicles
MX2009008470A (en) 2007-02-09 2009-11-26 Univ Northwestern Particles for detecting intracellular targets.
JP2010520749A (en) * 2007-02-27 2010-06-17 ノースウェスタン ユニバーシティ Binding molecules to nanoparticles
US8058415B2 (en) 2007-04-24 2011-11-15 The Board Of Trustees Of The University Of Illinois Aptamer- and nucleic acid enzyme-based systems for simultaneous detection of multiple analytes
US20080293588A1 (en) * 2007-05-11 2008-11-27 Northwestern University Nanodisk codes
AU2008259907B2 (en) 2007-05-30 2014-12-04 Northwestern University Nucleic acid functionalized nanoparticles for therapeutic applications
US7807372B2 (en) 2007-06-04 2010-10-05 Northwestern University Screening sequence selectivity of oligonucleotide-binding molecules using nanoparticle based colorimetric assay
US8409800B2 (en) 2007-07-16 2013-04-02 The Board Of Trustees Of The University Of Illinois Nucleic acid based fluorescent sensor for copper detection
US8568690B2 (en) 2007-07-31 2013-10-29 The Board Of Trustees Of The University Of Illinois MRI contrast agents and high-throughput screening by MRI
US8367416B2 (en) 2007-08-10 2013-02-05 The Board Of Trustees Of The University Of Illinois Nucleic acid based fluorescent sensor for mercury detection
US9097644B2 (en) 2007-08-17 2015-08-04 Massachusetts Institute Of Technology Magnetic resonance-based viscometers and methods
CA2700970A1 (en) 2007-09-28 2009-04-09 T2 Biosystems, Inc. Nmr diagnostics by means of a plastic sample container
US7829735B2 (en) * 2007-10-26 2010-11-09 Northwestern University Universal phosphoramidite for preparation of modified biomolecules and surfaces
US8519708B2 (en) 2007-11-06 2013-08-27 T2 Biosystems, Inc. Small magnet and RF coil for magnetic resonance relaxometry
US20090137405A1 (en) * 2007-11-16 2009-05-28 Christopher Bowman Detection of nucleic acid biomarkers using polymerization-based amplification
US20100105024A1 (en) * 2008-01-14 2010-04-29 Transgenex Nanobiotech, Inc. Rapid test including genetic sequence probe
US20090181361A1 (en) * 2008-01-14 2009-07-16 Weidong Xu Rapid test for detecting infection
US8174742B2 (en) 2008-03-14 2012-05-08 New York University System for applying optical forces from phase gradients
WO2009117798A2 (en) 2008-03-24 2009-10-01 Universidade Federal De Pernambuco - Ufpe Fluorescent nanoparticle composites themselves, process for the preparation of such composites, and use in rapid diagnosis systems with affinity to biological molecules
US8062893B2 (en) 2008-10-10 2011-11-22 The Board Of Trustees Of The University Of Illinois Fluorescent sensor for mercury
US9157974B2 (en) 2008-10-29 2015-10-13 T2 Biosystems, Inc. NMR detection of coagulation time
AU2009316286B2 (en) 2008-11-24 2016-05-26 Northwestern University Polyvalent RNA-nanoparticle compositions
US20100233270A1 (en) 2009-01-08 2010-09-16 Northwestern University Delivery of Oligonucleotide-Functionalized Nanoparticles
WO2010089098A1 (en) 2009-02-05 2010-08-12 Deklatec Gmbh Method and means for diagnosing tuberculosis
US10001476B2 (en) * 2009-10-28 2018-06-19 Genmark Diagnostics, Inc. Capture ligand controls, blocking probes, masking probes and methods of using the same
CN102666879B (en) 2009-10-30 2016-02-24 西北大学 Templated nanometer conjugate
US8445228B2 (en) 2009-11-16 2013-05-21 Massachusetts Institute Of Technology Enhancement of in vitro translation by nanoparticle conjugates
BRPI0912489B8 (en) * 2009-12-30 2019-08-20 Univ Minas Gerais carbon nanotube conjugate to inhibit plant pathogen infection structures
US20130034599A1 (en) 2010-01-19 2013-02-07 Northwestern University Synthetic nanostructures including nucleic acids and/or other entities
US8815156B2 (en) 2010-07-19 2014-08-26 Andalyze, Inc. Sensor housing and reagent chemistry
US8409807B2 (en) 2010-10-22 2013-04-02 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
CN103384724B (en) 2010-10-22 2017-11-17 T2生物系统公司 For testing and analyzing the NMR system and method for thing
EP2732046B1 (en) 2011-07-13 2017-09-06 T2 Biosystems, Inc. Nmr methods for monitoring blood clot formation
AU2012308302A1 (en) 2011-09-14 2014-03-20 Northwestern University Nanoconjugates able to cross the blood-brain barrier
US10620205B2 (en) 2011-09-21 2020-04-14 T2 Biosystems, Inc. NMR methods for endotoxin analysis
US9335292B2 (en) 2011-10-13 2016-05-10 Auburn University Electrochemical proximity assay
EP2594942A1 (en) * 2011-11-16 2013-05-22 Koninklijke Philips Electronics N.V. Long rigid spacers to enhance binding kinetics in immunoassays
US9051583B2 (en) 2011-12-19 2015-06-09 Northwestern University Modified silica shell particles, and methods of making and using the same
US9562271B2 (en) 2012-04-20 2017-02-07 T2 Biosystems, Inc. Compositions and methods for detection of Candida species
KR101974577B1 (en) * 2012-05-21 2019-05-02 삼성전자주식회사 Template for manufacturing nanoparticle and method for preparing nanoparticle using the same
US9801953B2 (en) 2012-10-15 2017-10-31 Emory University Nanoparticles carrying nucleic acid cassettes for expressing RNA
US9512468B2 (en) 2012-11-06 2016-12-06 Industrial Technology Research Institute Detection method uses magnetic and detectable nanoparticles with oligonucleotides attached thereto
CA2893591A1 (en) 2012-12-07 2014-06-12 T2 Biosystems, Inc. Methods for monitoring tight clot formation
US11366061B2 (en) 2013-01-24 2022-06-21 Grace Bio-Labs, Inc. Protein microarray assay imager
EP3024494A1 (en) 2013-07-25 2016-06-01 Exicure, Inc. Spherical nucleic acid-based constructs as immunoregulatory agents
WO2015066708A1 (en) 2013-11-04 2015-05-07 Northwestern University Quantification and spatio-temporal tracking of a target using a spherical nucleic acid (sna)
US10413565B2 (en) 2014-04-30 2019-09-17 Northwestern University Nanostructures for modulating intercellular communication and uses thereof
CN106535876B (en) 2014-06-04 2020-09-11 埃克西奎雷股份有限公司 Multivalent delivery of immunomodulators through liposomal spherical nucleic acids for prophylactic or therapeutic applications
WO2016004168A1 (en) * 2014-07-01 2016-01-07 Aurasense Therapeutics, Llc Spherical nanoparticles as antibacterial agents
KR20170072188A (en) 2014-09-23 2017-06-26 티어랩 리서치, 인코포레이티드 Systems and methods for integration of microfluidic tear collection and lateral flow analysis of analytes of interest
CA2963931A1 (en) 2014-10-06 2016-04-14 Exicure, Inc. Anti-tnf compounds
CN107208146B (en) 2014-11-20 2022-01-18 安普里怀斯公司 Compositions and methods for nucleic acid amplification
JP2017537619A (en) 2014-11-21 2017-12-21 ノースウェスタン ユニバーシティ Sequence-specific intracellular uptake of spherical nucleic acid nanoparticle complexes
WO2016113622A1 (en) 2015-01-12 2016-07-21 Nanomark Pesquisa E Desenvolvimento Tecnológico Ltda Magnetic nanocomposite retrieval of nucleotide sequence
CA3011901A1 (en) 2016-01-21 2017-07-27 T2 Biosystems, Inc. Nmr methods and systems for the rapid detection of bacteria
WO2017193087A1 (en) 2016-05-06 2017-11-09 Exicure, Inc. Liposomal spherical nucleic acid (sna) constructs prsenting antisense oligonucleotides(aso) for specific knockdown of interleukin 17 receptor mrna
US11364304B2 (en) 2016-08-25 2022-06-21 Northwestern University Crosslinked micellar spherical nucleic acids
US10852274B2 (en) 2017-03-09 2020-12-01 Auburn University Differential circuit for background correction in electrochemical measurements
US11696954B2 (en) 2017-04-28 2023-07-11 Exicure Operating Company Synthesis of spherical nucleic acids using lipophilic moieties
US11505799B2 (en) 2017-07-07 2022-11-22 Innamed, Inc. Aptamers for measuring lipoprotein levels
US11331019B2 (en) 2017-08-07 2022-05-17 The Research Foundation For The State University Of New York Nanoparticle sensor having a nanofibrous membrane scaffold
WO2019094670A1 (en) * 2017-11-10 2019-05-16 Dnp123 Company Molecular detection via programmable self-assembly
US11560565B2 (en) 2018-06-13 2023-01-24 Auburn University Electrochemical detection nanostructure, systems, and uses thereof
KR102239459B1 (en) * 2019-09-16 2021-04-12 한국화학연구원 Composition for temperature sensors including graphene oxide, DNAzyme and PNA and temperature sensing method using the same
CN111751355B (en) * 2020-07-22 2022-05-24 攀钢集团研究院有限公司 Method for determining content of ferrous iron ions in Fe (II) -EDTA (ethylene diamine tetraacetic acid) complex denitration liquid
WO2023196818A1 (en) 2022-04-04 2023-10-12 The Regents Of The University Of California Genetic complementation compositions and methods

Family Cites Families (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1190838A (en) * 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
US5288609A (en) 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
CA1260372A (en) * 1984-04-27 1989-09-26 Elazar Rabbani Hybridization method for the detection of genetic materials
TW203120B (en) * 1985-10-04 1993-04-01 Abbott Lab
US4996143A (en) * 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US5137827A (en) 1986-03-25 1992-08-11 Midwest Research Technologies, Inc. Diagnostic element for electrical detection of a binding reaction
US5360895A (en) 1987-04-22 1994-11-01 Associated Universities, Inc. Derivatized gold clusters and antibody-gold cluster conjugates
US5451463A (en) * 1989-08-28 1995-09-19 Clontech Laboratories, Inc. Non-nucleoside 1,3-diol reagents for labeling synthetic oligonucleotides
ES2116977T3 (en) * 1990-05-11 1998-08-01 Microprobe Corp SOLID SUPPORTS FOR NUCLEIC ACID HYBRIDIZATION TESTS AND METHODS TO IMMOBILIZE OLIGONUCLEOTIDES IN A COVALENT WAY.
US5460831A (en) * 1990-06-22 1995-10-24 The Regents Of The University Of California Targeted transfection nanoparticles
AU8951191A (en) * 1990-10-29 1992-05-26 Dekalb Plant Genetics Isolation of biological materials using magnetic particles
US5665582A (en) * 1990-10-29 1997-09-09 Dekalb Genetics Corp. Isolation of biological materials
JPH04197200A (en) * 1990-11-28 1992-07-16 Canon Inc Method of detecting nucleic acid
EP0613585A4 (en) 1991-11-22 1995-06-21 Univ California Semiconductor nanocrystals covalently bound to solid inorganic surfaces using self-assembled monolayers.
GB9212164D0 (en) 1992-06-09 1992-07-22 Medical Res Council Preparation of nucleic acids
US5472881A (en) 1992-11-12 1995-12-05 University Of Utah Research Foundation Thiol labeling of DNA for attachment to gold surfaces
US5384265A (en) * 1993-03-26 1995-01-24 Geo-Centers, Inc. Biomolecules bound to catalytic inorganic particles, immunoassays using the same
US5637508A (en) 1993-03-26 1997-06-10 Geo-Centers, Inc. Biomolecules bound to polymer or copolymer coated catalytic inorganic particles, immunoassays using the same and kits containing the same
KR960703174A (en) * 1993-06-09 1996-06-19 미안 알렉 MAGNETIC CYCLE REACTION
US5543158A (en) * 1993-07-23 1996-08-06 Massachusetts Institute Of Technology Biodegradable injectable nanoparticles
JPH07227299A (en) * 1994-02-14 1995-08-29 Kyoto Daiichi Kagaku:Kk Method for detecting specific base sequence of dna and device therefor
US5521289A (en) * 1994-07-29 1996-05-28 Nanoprobes, Inc. Small organometallic probes
US5599668A (en) 1994-09-22 1997-02-04 Abbott Laboratories Light scattering optical waveguide method for detecting specific binding events
CA2253710A1 (en) * 1996-04-25 1997-10-30 Spectrametrix Inc. Analyte assay using particulate labels
US6586193B2 (en) 1996-04-25 2003-07-01 Genicon Sciences Corporation Analyte assay using particulate labels
CA2262018C (en) 1996-07-29 2007-10-02 Nanosphere Llc Nanoparticles having oligonucleotides attached thereto and uses therefor
US6361944B1 (en) 1996-07-29 2002-03-26 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
WO1998010289A1 (en) 1996-09-04 1998-03-12 The Penn State Research Foundation Self-assembled metal colloid monolayers
JP2001503041A (en) 1996-10-23 2001-03-06 エスケーダヴリュ トロストベルグ アクチエンゲゼルシャフト Method for producing bioactive polymer nanoparticles-nucleic acid conjugate
US5830986A (en) 1996-10-28 1998-11-03 Massachusetts Institute Of Technology Methods for the synthesis of functionalizable poly(ethylene oxide) star macromolecules
US5900481A (en) 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
US5922537A (en) 1996-11-08 1999-07-13 N.o slashed.AB Immunoassay, Inc. Nanoparticles biosensor
US5939021A (en) 1997-01-23 1999-08-17 Hansen; W. Peter Homogeneous binding assay
AUPP004497A0 (en) 1997-10-28 1997-11-20 University Of Melbourne, The Stabilized particles
JP2002509443A (en) 1997-10-31 2002-03-26 ジェン−プローブ・インコーポレーテッド Nucleic acid detection method
US5990479A (en) 1997-11-25 1999-11-23 Regents Of The University Of California Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes
US5972615A (en) 1998-01-21 1999-10-26 Urocor, Inc. Biomarkers and targets for diagnosis, prognosis and management of prostate disease
US6287765B1 (en) 1998-05-20 2001-09-11 Molecular Machines, Inc. Methods for detecting and identifying single molecules
US6290839B1 (en) 1998-06-23 2001-09-18 Clinical Micro Sensors, Inc. Systems for electrophoretic transport and detection of analytes
US6406921B1 (en) 1998-07-14 2002-06-18 Zyomyx, Incorporated Protein arrays for high-throughput screening
US6251303B1 (en) 1998-09-18 2001-06-26 Massachusetts Institute Of Technology Water-soluble fluorescent nanocrystals
US6306610B1 (en) 1998-09-18 2001-10-23 Massachusetts Institute Of Technology Biological applications of quantum dots
US6203989B1 (en) 1998-09-30 2001-03-20 Affymetrix, Inc. Methods and compositions for amplifying detectable signals in specific binding assays
IL126776A (en) 1998-10-27 2001-04-30 Technion Res & Dev Foundation Method for gold depositions
EP1135682B1 (en) 1998-11-30 2007-07-11 Nanosphere, Inc. Nanoparticles with polymer shells
US6277489B1 (en) 1998-12-04 2001-08-21 The Regents Of The University Of California Support for high performance affinity chromatography and other uses
CA2376623C (en) 1999-06-25 2011-04-19 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
AU774593C (en) 2000-01-13 2005-06-23 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
ATE487136T1 (en) 2000-03-28 2010-11-15 Nanosphere Inc NANOPARTICLES WITH BONDED OLIGONUCLEOTIDES AND USES THEREOF
EP1279959A4 (en) 2000-05-11 2006-07-05 Toudai Tlo Ltd Polymer composition for forming surface of biosensor
AU7687001A (en) 2000-07-11 2002-01-21 Nanospherre Inc Method of detection by enhancement of silver staining
AU2001281248A1 (en) 2000-08-11 2002-03-13 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
CN1507357A (en) 2000-10-31 2004-06-23 PRҩƷ���޹�˾ Method and compositions for enhanced delivery of bioactive molecules
WO2002046483A2 (en) 2000-12-06 2002-06-13 Northwestern University Silver stain removal from dna detection chips by cyanide etching or sonication
EP1356109A2 (en) 2000-12-08 2003-10-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor

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EP1818417B1 (en) 2014-02-12
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JP2008029351A (en) 2008-02-14
JP4245664B2 (en) 2009-03-25
EP0918885A4 (en) 2001-11-14
DE69737598D1 (en) 2007-05-24
CA2262018C (en) 2007-10-02
JP2004194669A (en) 2004-07-15
EP0918885B1 (en) 2007-04-11
WO1998004740A1 (en) 1998-02-05
EP1818417A3 (en) 2010-01-20
JP2008067711A (en) 2008-03-27
US20020137058A1 (en) 2002-09-26
EP0918885A1 (en) 1999-06-02
ES2287956T3 (en) 2007-12-16
AU4043497A (en) 1998-02-20
US6773884B2 (en) 2004-08-10

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