CA2267101A1 - Thermostable nucleic acid polymerase from thermococcus gorgonarius - Google Patents

Thermostable nucleic acid polymerase from thermococcus gorgonarius

Info

Publication number
CA2267101A1
CA2267101A1 CA002267101A CA2267101A CA2267101A1 CA 2267101 A1 CA2267101 A1 CA 2267101A1 CA 002267101 A CA002267101 A CA 002267101A CA 2267101 A CA2267101 A CA 2267101A CA 2267101 A1 CA2267101 A1 CA 2267101A1
Authority
CA
Canada
Prior art keywords
nucleic acid
thermococcus gorgonarius
acid polymerase
thermostable nucleic
thermostable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002267101A
Other languages
French (fr)
Other versions
CA2267101C (en
Inventor
Waltraud Ankenbauer
Elizaveta Bonch-Osmolovskaya
Christine Ebenbichler
Bernhard Angerer
Gudrun Schmitz-Agheguian
Frank Laue
Vitaly Svetlichny
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics Gmbh
Waltraud Ankenbauer
Elizaveta Bonch-Osmolovskaya
Christine Ebenbichler
Bernhard Angerer
Gudrun Schmitz-Agheguian
Frank Laue
Vitaly Svetlichny
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP96115874A external-priority patent/EP0834570A1/en
Application filed by Roche Diagnostics Gmbh, Waltraud Ankenbauer, Elizaveta Bonch-Osmolovskaya, Christine Ebenbichler, Bernhard Angerer, Gudrun Schmitz-Agheguian, Frank Laue, Vitaly Svetlichny filed Critical Roche Diagnostics Gmbh
Publication of CA2267101A1 publication Critical patent/CA2267101A1/en
Application granted granted Critical
Publication of CA2267101C publication Critical patent/CA2267101C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Abstract

A purified thermostable enzyme is derived from the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90 % of its activity after incubation for two hours at 95 °C in the presence of stabilizing agents and possesses 3'-5' proofreading exonuclease activity. Thermostable DNA
polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).
CA002267101A 1996-10-03 1997-10-01 Thermostable nucleic acid polymerase from thermococcus gorgonarius Expired - Lifetime CA2267101C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP96115874A EP0834570A1 (en) 1996-10-03 1996-10-03 Thermostable nucleic acid polymerase from Thermococcus gorgonarius
EP96115874.8 1996-10-03
EP97100584A EP0834571A1 (en) 1996-10-03 1997-01-16 Thermostable nucleic acid polymerase from Thermococcus gorgonarius
EP97100584.8 1997-01-16
PCT/EP1997/005393 WO1998014590A1 (en) 1996-10-03 1997-10-01 THERMOSTABLE NUCLEIC ACID POLYMERASE FROM $i(THERMOCOCCUS GORGONARIUS)

Publications (2)

Publication Number Publication Date
CA2267101A1 true CA2267101A1 (en) 1998-04-09
CA2267101C CA2267101C (en) 2009-06-02

Family

ID=26142218

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002267101A Expired - Lifetime CA2267101C (en) 1996-10-03 1997-10-01 Thermostable nucleic acid polymerase from thermococcus gorgonarius

Country Status (11)

Country Link
US (3) US7425423B1 (en)
EP (2) EP0834571A1 (en)
JP (1) JP4308324B2 (en)
AT (1) ATE346937T1 (en)
AU (1) AU716933B2 (en)
CA (1) CA2267101C (en)
DE (1) DE69737024T2 (en)
IL (1) IL129167A0 (en)
NO (1) NO991566L (en)
NZ (1) NZ334653A (en)
WO (1) WO1998014590A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1925669B1 (en) 2000-02-17 2010-12-08 Qiagen GmbH Thermostable chimeric nucleic acid polymerases and uses thereof
DE10049211A1 (en) * 2000-10-05 2002-04-18 Qiagen Gmbh Thermostable polymerase from Thermococcus pacificus
KR20010079080A (en) * 2001-06-12 2001-08-22 김유삼 Nucleotide sequences and amino acid sequences encoding a DNA polymerase
EP1275735A1 (en) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
JP4610856B2 (en) * 2003-02-06 2011-01-12 Ｎｏｋ株式会社 Composition for fluororubber-based sealing material and fluororubber-based sealing material
KR20090132645A (en) 2004-06-04 2009-12-30 다카라 바이오 가부시키가이샤 Polypeptides having dna polymerase activity
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
FI3461807T3 (en) 2011-06-08 2023-09-07 Life Technologies Corp Design and development of novel detergents for use in pcr systems
EP3539944A1 (en) 2013-10-25 2019-09-18 Life Technologies Corporation Novel compounds for use in pcr systems and applications thereof
US10411958B2 (en) * 2014-09-08 2019-09-10 Intel Corporation Automatic device configuration
CN106754816B (en) * 2017-02-24 2020-10-27 依科赛生物科技（太仓）有限公司 High-fidelity rapid amplification fusion enzyme and preparation method thereof

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES8706823A1 (en) 1985-03-28 1987-06-16 Cetus Corp Process for amplifying, detecting, and/or cloning nucleic acid sequences.
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
CA1338457C (en) 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US4889819A (en) 1988-05-20 1989-12-26 International Business Machines Corporation Method for fabricating shallow junctions by preamorphizing with dopant of same conductivity as substrate
US5322785A (en) 1990-04-26 1994-06-21 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis
US5352778A (en) 1990-04-26 1994-10-04 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
US5756334A (en) * 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
WO1992009689A1 (en) * 1990-12-03 1992-06-11 Stratagene PURIFIED THERMOSTABLE $i(PYROCOCCUS FURIOSUS)
DE69218359T2 (en) 1991-12-12 1997-10-09 Nippon Telegraph & Telephone Method and circuit for noise shaping
JPH067160A (en) 1991-12-18 1994-01-18 New England Biolabs Inc Recombinant thermally stable dna polymerase from archaebacteria
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension

Also Published As

Publication number Publication date
EP0931151A1 (en) 1999-07-28
AU4707197A (en) 1998-04-24
WO1998014590A1 (en) 1998-04-09
JP2001502898A (en) 2001-03-06
US8008054B2 (en) 2011-08-30
US20090093043A1 (en) 2009-04-09
DE69737024D1 (en) 2007-01-11
JP4308324B2 (en) 2009-08-05
ATE346937T1 (en) 2006-12-15
NO991566D0 (en) 1999-03-30
EP0834571A1 (en) 1998-04-08
US7425423B1 (en) 2008-09-16
US20110020898A1 (en) 2011-01-27
DE69737024T2 (en) 2007-06-21
US7759107B2 (en) 2010-07-20
CA2267101C (en) 2009-06-02
EP0931151B1 (en) 2006-11-29
NO991566L (en) 1999-03-30
IL129167A0 (en) 2000-02-17
NZ334653A (en) 2000-11-24
AU716933B2 (en) 2000-03-09

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Effective date: 20171002