CA2275489A1 - Oil bodies and associated proteins as affinity matrices - Google Patents

Oil bodies and associated proteins as affinity matrices Download PDF

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Publication number
CA2275489A1
CA2275489A1 CA002275489A CA2275489A CA2275489A1 CA 2275489 A1 CA2275489 A1 CA 2275489A1 CA 002275489 A CA002275489 A CA 002275489A CA 2275489 A CA2275489 A CA 2275489A CA 2275489 A1 CA2275489 A1 CA 2275489A1
Authority
CA
Canada
Prior art keywords
oil body
oil
oil bodies
associated proteins
affinity matrices
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002275489A
Other languages
French (fr)
Other versions
CA2275489C (en
Inventor
Maurice Moloney
Joseph Boothe
Gijs Van Rooijen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SemBioSys Genetics Inc
Original Assignee
Sembiosys Genetics Inc.
Maurice Moloney
Joseph Boothe
Gijs Van Rooijen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sembiosys Genetics Inc., Maurice Moloney, Joseph Boothe, Gijs Van Rooijen filed Critical Sembiosys Genetics Inc.
Publication of CA2275489A1 publication Critical patent/CA2275489A1/en
Application granted granted Critical
Publication of CA2275489C publication Critical patent/CA2275489C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01G—COMPOUNDS CONTAINING METALS NOT COVERED BY SUBCLASSES C01D OR C01F
    • C01G11/00—Compounds of cadmium
    • C01G11/003—Preparation involving a liquid-liquid extraction, an adsorption or an ion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B1/00—Preparatory treatment of cellulose for making derivatives thereof, e.g. pre-treatment, pre-soaking, activation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6429—Thrombin (3.4.21.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21005—Thrombin (3.4.21.5)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Inorganic Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Materials Engineering (AREA)
  • Botany (AREA)
  • Polymers & Plastics (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method for the separation of a target molecule from a mixture is described.
The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions.
CA002275489A 1996-12-16 1997-12-05 Oil bodies and associated proteins as affinity matrices Expired - Fee Related CA2275489C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/767,026 1996-12-16
US08/767,026 US5856452A (en) 1996-12-16 1996-12-16 Oil bodies and associated proteins as affinity matrices
PCT/CA1997/000951 WO1998027115A1 (en) 1996-12-16 1997-12-05 Oil bodies and associated proteins as affinity matrices

Publications (2)

Publication Number Publication Date
CA2275489A1 true CA2275489A1 (en) 1998-06-25
CA2275489C CA2275489C (en) 2007-03-27

Family

ID=25078275

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002275489A Expired - Fee Related CA2275489C (en) 1996-12-16 1997-12-05 Oil bodies and associated proteins as affinity matrices

Country Status (19)

Country Link
US (5) US5856452A (en)
EP (1) EP1007554B1 (en)
JP (2) JP3833719B2 (en)
KR (1) KR100421935B1 (en)
CN (1) CN1325513C (en)
AR (1) AR010780A1 (en)
AT (1) ATE321777T1 (en)
AU (1) AU739339B2 (en)
BR (1) BR9713727B1 (en)
CA (1) CA2275489C (en)
DE (1) DE69735599T2 (en)
DK (1) DK1007554T3 (en)
ES (1) ES2259193T3 (en)
HK (1) HK1025977A1 (en)
IL (1) IL130403A (en)
NZ (1) NZ336558A (en)
TW (1) TW589322B (en)
WO (1) WO1998027115A1 (en)
ZA (1) ZA9711237B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022180211A1 (en) * 2021-02-26 2022-09-01 Société des Produits Nestlé S.A. Low ph co-extraction method of preparing a stable oil body solution

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022180211A1 (en) * 2021-02-26 2022-09-01 Société des Produits Nestlé S.A. Low ph co-extraction method of preparing a stable oil body solution

Also Published As

Publication number Publication date
CN1325513C (en) 2007-07-11
DE69735599T2 (en) 2006-08-31
AU739339B2 (en) 2001-10-11
US20030059910A1 (en) 2003-03-27
US6924363B1 (en) 2005-08-02
EP1007554A1 (en) 2000-06-14
US5856452A (en) 1999-01-05
CA2275489C (en) 2007-03-27
US7332587B2 (en) 2008-02-19
WO1998027115A1 (en) 1998-06-25
TW589322B (en) 2004-06-01
HK1025977A1 (en) 2000-12-01
BR9713727A (en) 2000-01-25
AR010780A1 (en) 2000-07-12
ZA9711237B (en) 1998-07-06
ATE321777T1 (en) 2006-04-15
IL130403A0 (en) 2000-06-01
DE69735599D1 (en) 2006-05-18
ES2259193T3 (en) 2006-09-16
KR20000069499A (en) 2000-11-25
US6509453B1 (en) 2003-01-21
EP1007554B1 (en) 2006-03-29
BR9713727B1 (en) 2009-01-13
IL130403A (en) 2005-05-17
US20030096320A1 (en) 2003-05-22
DK1007554T3 (en) 2006-05-01
NZ336558A (en) 2001-08-31
CN1245503A (en) 2000-02-23
JP2001506241A (en) 2001-05-15
JP2006306881A (en) 2006-11-09
AU5220498A (en) 1998-07-15
KR100421935B1 (en) 2004-03-10
JP3833719B2 (en) 2006-10-18

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