CA2295324A1 - In vitro sorting method - Google Patents

In vitro sorting method Download PDF

Info

Publication number
CA2295324A1
CA2295324A1 CA002295324A CA2295324A CA2295324A1 CA 2295324 A1 CA2295324 A1 CA 2295324A1 CA 002295324 A CA002295324 A CA 002295324A CA 2295324 A CA2295324 A CA 2295324A CA 2295324 A1 CA2295324 A1 CA 2295324A1
Authority
CA
Canada
Prior art keywords
gene product
genetic elements
genetic
microcapsule
microcapsules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002295324A
Other languages
French (fr)
Other versions
CA2295324C (en
Inventor
Andrew Griffiths
Dan Tawfik
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
United Kingdom Research and Innovation
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26311843&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2295324(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from GBGB9714300.2A external-priority patent/GB9714300D0/en
Priority claimed from GBGB9806393.6A external-priority patent/GB9806393D0/en
Application filed by Individual filed Critical Individual
Priority to CA2792122A priority Critical patent/CA2792122C/en
Priority to CA002584453A priority patent/CA2584453A1/en
Publication of CA2295324A1 publication Critical patent/CA2295324A1/en
Application granted granted Critical
Publication of CA2295324C publication Critical patent/CA2295324C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1062—Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

Abstract

The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; (b) expressing the genetic elements to produce their respective gene products within the microcapsules; (c) sorting the genetic elements which produce the gene product having a desired activity. The invention enables the in vitro evolution of nucleic acids by repeated mutagenesis and iterative applications of the method of the invention.

Claims (29)

1. A method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of:
(a) compartmentalising the genetic elements into microcapsules;
(b) expressing the genetic elements to produce their respective gene products within the microcapsules;
(c) sorting the genetic elements which produce gene product(s) having the desired activity.
2. A method according to claim 1 wherein a gene product with the desired activity induces a change in the microcapsule which enables the microcapsule containing the gene product and the genetic element encoding it to be sorted.
3. A method according to claim 2 wherein a gene product having the desired activity modifies one or more molecules within the microcapsule which enables the microcapsule containing the gene product and the genetic element encoding it to be sorted.
4. A method according to claim 2 or claim 3 wherein the gene product having the desired activity induces a change in the optical properties of the microcapsule and the microcapsules are sorted thereby.
5. A method according to claim 1, wherein in step (b) the activity of the desired gene product within the microcapsule results, directly or indirectly, in the modification of the genetic element encoding the gene product to enable the isolation of the genetic element.
6. A method according to claim 5 wherein the gene product having the desired activity induces a change in the microcapsule which, when detected, triggers the modification of the genetic element within the compartment to enable the isolation of the genetic element.
7. A method according to claim 6 wherein the change in the microcapsule is a change in the optical properties thereof.
8. A method according to claim 5 wherein a part of the genetic element is a substrate and the activity of the desired gene product within the microcapsule results, directly or indirectly, in the conversion of said substrate into a product which remains part of the genetic element and enables its isolation.
9. A method according to claim 5 wherein a part of the genetic element is a ligand and the desired gene product within the microcapsule binds, directly or indirectly, to said ligand to enable the isolation of the genetic element.
10. A method according to any preceding claim wherein the activity of the desired gene product within the microcapsule results, directly or indirectly, in the alteration of the expression of a second gene within the compartment and the activity of the product of the said second gene enables the isolation of the genetic element.
11. A method according to claim 1, wherein step (b) comprises:
expressing the genetic elements to produce their respective gene products within the microcapsules, linking the gene products to the genetic elements encoding them and isolating the complexes thereby formed.
12. A method according to claim 11, wherein in step (c) the complexes are directly sorted to isolate genetic elements encoding a gene product having the desired activity.
13. A method according to claim 12, wherein the complexes are subjected to a further compartmentalisation step in order to isolate the genetic elements encoding a gene product having the desired activity.
14. A method according to claim 13 wherein the microcapsules are sorted according to claims 2 to 4.
15. A method according to claim 12 wherein the genetic elements are sorted according to claims 5 to 10.
16. A method according to any preceding claim, wherein the genetic elements are isolated from a library of genetic elements encoding a repertoire of gene products.
17. A method according to any preceding claim further comprising the additional step of:
(d) introducing one or more mutations into the genetic element(s) isolated in step (c).
18. A method according to any preceding claim further comprising iteratively repeating one or more of steps (a) to (d).
19. A method according to any preceding claim further comprising amplifying the genetic elements.
20. A method for compartmentalising a genetic element and expressing the genetic element to form its gene product within the compartment, comprising the steps of:
(a) forming an aqueous solution comprising the genetic element and the components necessary to express it to form its gene product;
(b) microencapsulating the solution so as to form a discrete microcapsule comprising the genetic element; and (c) exposing the microcapsule to conditions suitable for the expression of the genetic element to form its gene product to proceed.
21. A method according to claim 20, wherein a library of genetic elements encoding a repertoire of gene products is encapsulated and each genetic element expressed to form its gene product within a microcapsule.
22. A method according to claim 20 or claim 21, wherein microencapsulation is achieved by forming a water-in-oil emulsion of the aqueous solution in an oil-based medium.
23. A microcapsule obtainable by the method of any one of claims 20 to 22.
24. A method according to any one of claims 5 to 19, wherein the genetic elements are sorted by affinity purification.
25. A method according to any one of claims 5 to 19, wherein the genetic elements are sorted by selective ablation of genetic elements which do not encode the desired gene product.
26. A product when isolated according to the method of any preceding claim.
27. A method for preparing a gene product, comprising the steps of:
(a) preparing a genetic element encoding the gene product;
(b) compartmentalising genetic elements into microcapsules;
(c) expressing the genetic elements to produce their respective gene products within the microcapsules;
(d) sorting the genetic elements which produce the gene product(s) having the desired activity; and (e) expressing the gene product having the desired activity.
28. A method for screening a compound or compounds capable of modulating the activity of a gene product, comprising the steps of:
(a) preparing a repertoire of genetic elements encoding gene product;

(b) compartmentalising the genetic elements into microcapsules;
(c) expressing the genetic elements to produce their respective gene products within the microcapsules;
(d) sorting the genetic elements which produce the gene product(s) having the desired activity; and (e) contacting a gene product having the desired activity with the compound or compounds and monitoring the modulation of an activity of the gene product by the compound or compounds.
29. A method for preparing a compound or compounds comprising the steps of:
(a) providing a synthesis protocol wherein at least one step is facilitated by a polypeptide;
(b) preparing genetic elements encoding variants of the polypeptide which facilitates this step;
(c) compartmentalising the genetic elements into microcapsules;
(d) expressing the genetic elements to produce their respective gene products within the microcapsules;
(e) sorting the genetic elements which produce polypeptide gene product(s) having the desired activity; and (f) preparing the compound or compounds using the polypeptide gene product identified in (e) to facilitate the relevant step of the synthesis.
CA2295324A 1997-07-07 1998-06-29 In vitro sorting method Expired - Lifetime CA2295324C (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA2792122A CA2792122C (en) 1997-07-07 1998-06-29 In vitro sorting method
CA002584453A CA2584453A1 (en) 1997-07-07 1998-06-29 A method for increasing the concentration of nucleic acids

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB9714300.2 1997-07-07
GBGB9714300.2A GB9714300D0 (en) 1997-07-07 1997-07-07 In vitro sorting method
GB9806393.6 1998-03-25
GBGB9806393.6A GB9806393D0 (en) 1998-03-25 1998-03-25 In vitro sorting method
PCT/GB1998/001889 WO1999002671A1 (en) 1997-07-07 1998-06-29 In vitro sorting method

Related Child Applications (2)

Application Number Title Priority Date Filing Date
CA002584453A Division CA2584453A1 (en) 1997-07-07 1998-06-29 A method for increasing the concentration of nucleic acids
CA2792122A Division CA2792122C (en) 1997-07-07 1998-06-29 In vitro sorting method

Publications (2)

Publication Number Publication Date
CA2295324A1 true CA2295324A1 (en) 1999-01-21
CA2295324C CA2295324C (en) 2012-12-18

Family

ID=26311843

Family Applications (3)

Application Number Title Priority Date Filing Date
CA2792122A Expired - Lifetime CA2792122C (en) 1997-07-07 1998-06-29 In vitro sorting method
CA002584453A Abandoned CA2584453A1 (en) 1997-07-07 1998-06-29 A method for increasing the concentration of nucleic acids
CA2295324A Expired - Lifetime CA2295324C (en) 1997-07-07 1998-06-29 In vitro sorting method

Family Applications Before (2)

Application Number Title Priority Date Filing Date
CA2792122A Expired - Lifetime CA2792122C (en) 1997-07-07 1998-06-29 In vitro sorting method
CA002584453A Abandoned CA2584453A1 (en) 1997-07-07 1998-06-29 A method for increasing the concentration of nucleic acids

Country Status (13)

Country Link
US (7) US6489103B1 (en)
EP (6) EP1482036B1 (en)
JP (3) JP4298912B2 (en)
AT (4) ATE278013T1 (en)
AU (1) AU736836B2 (en)
CA (3) CA2792122C (en)
CY (1) CY1111537T1 (en)
DE (4) DE69838521T2 (en)
DK (3) DK1801214T3 (en)
ES (4) ES2294427T3 (en)
GB (1) GB2342094B (en)
PT (2) PT1801214E (en)
WO (1) WO1999002671A1 (en)

Families Citing this family (349)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7262055B2 (en) 1998-08-25 2007-08-28 Gendaq Limited Regulated gene expression in plants
US7285416B2 (en) 2000-01-24 2007-10-23 Gendaq Limited Regulated gene expression in plants
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
EP3034626A1 (en) 1997-04-01 2016-06-22 Illumina Cambridge Limited Method of nucleic acid sequencing
CA2792122C (en) 1997-07-07 2015-09-08 Medical Research Council In vitro sorting method
AR021833A1 (en) 1998-09-30 2002-08-07 Applied Research Systems METHODS OF AMPLIFICATION AND SEQUENCING OF NUCLEIC ACID
AU2004242526B2 (en) * 1999-01-07 2007-08-30 United Kingdom Research And Innovation Optical Sorting Method
GB9900298D0 (en) * 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
WO2000056901A2 (en) 1999-03-24 2000-09-28 Board Of Regents, The University Of Texas System Linear and circular expression elements
GB9917027D0 (en) 1999-07-20 1999-09-22 Affibody Technology Sweeden Ab In vitro selection and optional identification of polypeptides using solid support carriers
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
GB0022458D0 (en) * 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
AR031640A1 (en) 2000-12-08 2003-09-24 Applied Research Systems ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS IN A SOLID SUPPORT
US20050118580A1 (en) * 2001-03-16 2005-06-02 Helmut Merk Nucleic acid which is stabilized against decomposition
JP4838957B2 (en) * 2001-05-02 2011-12-14 独立行政法人理化学研究所 Protein production method using cell-free protein synthesis system
GB0114854D0 (en) * 2001-06-18 2001-08-08 Medical Res Council Selective gene amplification
GB0115841D0 (en) 2001-06-28 2001-08-22 Medical Res Council Ligand
WO2003037302A1 (en) * 2001-10-30 2003-05-08 Windsor J Brian Method and system for the co-isolation of cognate dna, rna and protein sequences and method for screening co-isolates for defined activities
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
WO2003097829A1 (en) 2002-05-22 2003-11-27 Riken Process for producing protein in cell-free protein synthesis system using thioredoxin fused protein expression vector
AUPS298102A0 (en) * 2002-06-13 2002-07-04 Nucleics Pty Ltd Method for performing chemical reactions
JP2006507921A (en) * 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Method and apparatus for fluid dispersion
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
US20060002935A1 (en) 2002-06-28 2006-01-05 Domantis Limited Tumor Necrosis Factor Receptor 1 antagonists and methods of use therefor
KR101051245B1 (en) 2002-09-06 2011-07-21 이소제니카 리미티드 In Vitro Peptide Expression Libraries
GB0221053D0 (en) * 2002-09-11 2002-10-23 Medical Res Council Single-molecule in vitro evolution
JP4441170B2 (en) 2002-11-28 2010-03-31 独立行政法人理化学研究所 Escherichia coli cell extract having mutation in S12 ribosomal protein and cell-free protein production method using the same
GB0228819D0 (en) * 2002-12-11 2003-01-15 British American Tobacco Co Improvements relating to smoking articles
GB0230203D0 (en) 2002-12-27 2003-02-05 Domantis Ltd Fc fusion
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
AU2004254552B2 (en) 2003-01-29 2008-04-24 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
GB0307428D0 (en) * 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307403D0 (en) * 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
FI20030615A0 (en) * 2003-04-24 2003-04-24 Marko Virta Nanoparticle for Bioaffinity Assays
EP1610888A2 (en) 2003-04-10 2006-01-04 President And Fellows Of Harvard College Formation and control of fluidic species
EP1627062A1 (en) 2003-05-14 2006-02-22 Domantis Limited A process for recovering polypeptides that unfold reversibly from a polypeptide repertoire
EP2918595B1 (en) 2003-07-05 2019-12-11 The Johns-Hopkins University Method and compositions for detection and enumeration of genetic variations
BRPI0414004A (en) * 2003-08-27 2006-10-24 Harvard College electronic control of fluidic species
WO2005030957A1 (en) * 2003-10-01 2005-04-07 ETH Zürich Method for in vitro evolution of polypeptides
GB0325653D0 (en) 2003-11-03 2003-12-10 Medical Res Council CST emulsions
EP1691792A4 (en) * 2003-11-24 2008-05-28 Yeda Res & Dev Compositions and methods for in vitro sorting of molecular and cellular libraries
WO2005073410A2 (en) 2004-01-28 2005-08-11 454 Corporation Nucleic acid amplification with continuous flow emulsion
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US9477233B2 (en) 2004-07-02 2016-10-25 The University Of Chicago Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets
GB0418651D0 (en) 2004-08-20 2004-09-22 Medical Res Council Method
GB0419628D0 (en) 2004-09-03 2004-10-06 European Molecular Biology Lab Embl Method for determining protein solubility
RU2401842C2 (en) 2004-10-08 2010-10-20 Домантис Лимитед Antagonists and method of using said antagonists
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20080004436A1 (en) * 2004-11-15 2008-01-03 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Directed Evolution and Selection Using in Vitro Compartmentalization
JP4868731B2 (en) 2004-11-17 2012-02-01 独立行政法人理化学研究所 Cell-free protein synthesis system derived from cultured mammalian cells
JP4787488B2 (en) 2004-11-19 2011-10-05 独立行政法人理化学研究所 Cell-free protein synthesis method using linear template DNA and cell extract therefor
EP1844162B1 (en) 2005-02-01 2014-10-15 Applied Biosystems, LLC Method for identifying a sequence in a polynucleotide
EP2233582A1 (en) 2005-02-01 2010-09-29 AB Advanced Genetic Analysis Corporation Nucleic acid sequencing by performing successive cycles of duplex extension
CA2599683A1 (en) 2005-03-04 2006-09-14 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
US20070054119A1 (en) * 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
US20060228721A1 (en) * 2005-04-12 2006-10-12 Leamon John H Methods for determining sequence variants using ultra-deep sequencing
DE602006015633D1 (en) 2005-04-29 2010-09-02 Synthetic Genomics Inc AMPLIFICATION AND CLONING OF INDIVIDUAL DNA MOLECULES BY ROLLING CIRCLE AMPLIFICATION
EP1910537A1 (en) 2005-06-06 2008-04-16 454 Life Sciences Corporation Paired end sequencing
US20090233291A1 (en) * 2005-06-06 2009-09-17 454 Life Sciences Corporation Paired end sequencing
SG162795A1 (en) 2005-06-15 2010-07-29 Callida Genomics Inc Single molecule arrays for genetic and chemical analysis
WO2007021944A2 (en) 2005-08-11 2007-02-22 The J. Craig Venter Institute In vitro recombination method
JP5033949B2 (en) * 2005-08-12 2012-09-26 国立大学法人豊橋技術科学大学 Electric emulsification method
WO2007024778A2 (en) * 2005-08-22 2007-03-01 Applera Corporation Device, system and method for depositing processed immiscible-fluid-discrete-volumes
WO2007044245A2 (en) 2005-10-07 2007-04-19 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
KR100828714B1 (en) 2005-10-25 2008-05-09 주식회사 엘지화학 A method for multiplexing amplification using ribonucleotides
EP1801212A1 (en) * 2005-12-21 2007-06-27 Eidgenössische Technische Hochschule Zürich Selection of biocatalysts for chemical synthesis
EP2363205A3 (en) 2006-01-11 2014-06-04 Raindance Technologies, Inc. Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors
DE602007009811D1 (en) * 2006-01-27 2010-11-25 Harvard College COALESCENCE FLUIDER DROPLET
SG10201405158QA (en) 2006-02-24 2014-10-30 Callida Genomics Inc High throughput genome sequencing on dna arrays
CN101432439B (en) 2006-02-24 2013-07-24 考利达基因组股份有限公司 High throughput genome sequencing on DNA arrays
AU2007237909A1 (en) * 2006-04-19 2007-10-25 Applied Biosystems, Llc. Reagents, methods, and libraries for gel-free bead-based sequencing
EP2530168B1 (en) 2006-05-11 2015-09-16 Raindance Technologies, Inc. Microfluidic Devices
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2636755A1 (en) * 2006-05-26 2013-09-11 AltheaDx Incorporated Biochemical analysis of partitioned cells
WO2008021123A1 (en) 2006-08-07 2008-02-21 President And Fellows Of Harvard College Fluorocarbon emulsion stabilizing surfactants
EP2071927A2 (en) 2006-09-28 2009-06-24 Illumina, Inc. Compositions and methods for nucleotide sequencing
US7910354B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US7682791B2 (en) * 2006-10-29 2010-03-23 Macevicz Stephen C Method of generating nested sets of double stranded DNA circles
US20090111705A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by hybrid capture
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
DE602008003757D1 (en) 2007-02-23 2011-01-13 New England Biolabs Inc SELECTION AND ENRICHMENT OF PROTEINS BY IN VITRO LIBRARY
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
CA2679511A1 (en) * 2007-03-16 2008-09-25 454 Life Sciences Corporation System and method for detection of hiv drug resistant variants
CN102014871A (en) * 2007-03-28 2011-04-13 哈佛大学 Emulsions and techniques for formation
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US7883265B2 (en) * 2007-06-01 2011-02-08 Applied Biosystems, Llc Devices, systems, and methods for preparing emulsions
JP2010528608A (en) * 2007-06-01 2010-08-26 ４５４ ライフ サイエンシーズ コーポレイション System and method for identifying individual samples from complex mixtures
CA2683791A1 (en) 2007-06-06 2008-12-11 Domantis Limited Polypeptides, antibody variable domains & antagonists
US20090053724A1 (en) * 2007-06-28 2009-02-26 454 Life Sciences Corporation System and method for adaptive reagent control in nucleic acid sequencing
EP2212434A1 (en) * 2007-10-01 2010-08-04 Applied Biosystems Inc. Chase ligation sequencing
JP5704677B2 (en) 2007-11-05 2015-04-22 独立行政法人理化学研究所 Method for producing membrane protein
EP2215250B1 (en) 2007-11-27 2013-02-27 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
CN104650235A (en) 2007-11-30 2015-05-27 葛兰素集团有限公司 Antigen-Binding Constructs
US9797010B2 (en) 2007-12-21 2017-10-24 President And Fellows Of Harvard College Systems and methods for nucleic acid sequencing
WO2009100080A1 (en) 2008-02-04 2009-08-13 Life Technologies Corporation Composition and method for sequencing nucleic acid
US20090203086A1 (en) * 2008-02-06 2009-08-13 454 Life Sciences Corporation System and method for improved signal detection in nucleic acid sequencing
EA018471B1 (en) 2008-03-31 2013-08-30 Глаксо Груп Лимитед Drug fusions and conjugates
US8557742B2 (en) 2008-04-04 2013-10-15 Novozymes A/S Method of enhancing enzyme activity
GB0806562D0 (en) 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid
US7888034B2 (en) 2008-07-01 2011-02-15 454 Life Sciences Corporation System and method for detection of HIV tropism variants
WO2010003132A1 (en) 2008-07-02 2010-01-07 Illumina Cambridge Ltd. Using populations of beads for the fabrication of arrays on surfaces
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
WO2010033200A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US8663920B2 (en) 2011-07-29 2014-03-04 Bio-Rad Laboratories, Inc. Library characterization by digital assay
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
WO2010038042A1 (en) 2008-10-02 2010-04-08 Illumina Cambridge Ltd. Nucleic acid sample enrichment for sequencing applications
US20100261189A1 (en) * 2008-10-03 2010-10-14 Roche Molecular Systems, Inc. System and method for detection of HLA Variants
US20100087325A1 (en) * 2008-10-07 2010-04-08 Illumina, Inc. Biological sample temperature control system and method
US8541207B2 (en) 2008-10-22 2013-09-24 Illumina, Inc. Preservation of information related to genomic DNA methylation
US8486865B2 (en) * 2008-11-03 2013-07-16 The Regents Of The University Of California Methods for detecting modification resistant nucleic acids
WO2010053587A2 (en) 2008-11-07 2010-05-14 Mlc Dx Incorporated Methods of monitoring conditions by sequence analysis
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
EP2364326B1 (en) 2008-12-05 2018-07-04 Glaxo Group Limited Methods for selecting protease resistant polypeptides
GB0822260D0 (en) 2008-12-05 2009-01-14 Merten Christoph Assay
GB0822261D0 (en) 2008-12-05 2009-01-14 Merten Christoph Assay
US8748094B2 (en) 2008-12-19 2014-06-10 President And Fellows Of Harvard College Particle-assisted nucleic acid sequencing
EP2379748A4 (en) * 2008-12-23 2012-08-29 Illumina Inc Multibase delivery for long reads in sequencing by synthesis protocols
EP2387627B1 (en) 2009-01-15 2016-03-30 Adaptive Biotechnologies Corporation Adaptive immunity profiling and methods for generation of monoclonal antibodies
US8581209B2 (en) * 2009-01-29 2013-11-12 Southwest Research Institute Fluorescent monitoring of microcapsule oxidation
US20110301335A1 (en) 2009-02-19 2011-12-08 Stephen Duffield Anti-tnfr1 polypeptides, antibody variable domains & antagonists
CA2753332A1 (en) 2009-02-24 2010-09-02 Glaxo Group Limited Antigen-binding constructs
CA2753287A1 (en) 2009-02-24 2010-09-02 Glaxo Group Limited Antigen-binding constructs
CA2753263A1 (en) 2009-02-24 2010-09-02 Glaxo Group Limited Multivalent and/or multispecific rankl-binding constructs
EP2406003A2 (en) 2009-03-13 2012-01-18 President and Fellows of Harvard College Scale-up of flow-focusing microfluidic devices
EP3415235A1 (en) 2009-03-23 2018-12-19 Raindance Technologies Inc. Manipulation of microfluidic droplets
GB0904957D0 (en) 2009-03-23 2009-05-06 Univ Erasmus Medical Ct Tumour gene profile
UA98911C2 (en) 2009-03-27 2012-06-25 Ґлаксо Ґруп Лімітед Drug fusions and conjugates of insulinotropic peptides and antibody domain binding serum albumin
WO2010117457A2 (en) * 2009-04-08 2010-10-14 Applied Biosystems, Llc System comprising dual-sided thermal cycler and emulsion pcr in pouch
WO2010129541A1 (en) * 2009-05-04 2010-11-11 Affomix Corporation Emulsion selection of antibodies
RU2539032C2 (en) 2009-06-25 2015-01-10 Фред Хатчинсон Кансэр Рисёч Сентер Method for measuring artificial immunity
WO2011006914A2 (en) 2009-07-16 2011-01-20 Glaxo Group Limited Antagonists, uses & methods for partially inhibiting tnfr1
JP5099455B2 (en) * 2009-07-16 2012-12-19 独立行政法人科学技術振興機構 Method for selective amplification of RNA using emulsion
CA2767056C (en) 2009-09-02 2018-12-04 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
EP2473263B1 (en) 2009-09-02 2022-11-02 President and Fellows of Harvard College Multiple emulsions created using jetting and other techniques
US8182994B2 (en) 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
US20120276098A1 (en) 2009-09-30 2012-11-01 Bruce Hamilton Drug fusions and conjugates with extended half life
WO2011042564A1 (en) 2009-10-09 2011-04-14 Universite De Strasbourg Labelled silica-based nanomaterial with enhanced properties and uses thereof
US8609339B2 (en) * 2009-10-09 2013-12-17 454 Life Sciences Corporation System and method for emulsion breaking and recovery of biological elements
GB0918564D0 (en) 2009-10-22 2009-12-09 Plasticell Ltd Nested cell encapsulation
EP2493619B1 (en) 2009-10-27 2018-12-19 President and Fellows of Harvard College Droplet creation techniques
JP2013507978A (en) 2009-10-27 2013-03-07 グラクソ グループ リミテッド Stable anti-TNFR1 polypeptides, antibody variable domains and antagonists
US8524450B2 (en) * 2009-10-30 2013-09-03 Illumina, Inc. Microvessels, microparticles, and methods of manufacturing and using the same
EP2333102A1 (en) * 2009-12-14 2011-06-15 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method for identifying RNA ligands
EP2517025B1 (en) 2009-12-23 2019-11-27 Bio-Rad Laboratories, Inc. Methods for reducing the exchange of molecules between droplets
EP2523686A2 (en) 2010-01-14 2012-11-21 Glaxo Group Limited Liver targeting domain antibodies
ES2596655T3 (en) 2010-02-01 2017-01-11 Illumina Inc.  Focusing procedures and optical systems and assemblies that use them
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
EP2534267B1 (en) 2010-02-12 2018-04-11 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US8481903B2 (en) 2010-03-06 2013-07-09 Alexander Triener Systems, methods, and apparatuses including a moveable optical component for detecting optical signals from a sample
BR112012023441A2 (en) * 2010-03-17 2016-05-24 Basf Se fusion emulsification
CA2767182C (en) 2010-03-25 2020-03-24 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
CA2767113A1 (en) 2010-03-25 2011-09-29 Bio-Rad Laboratories, Inc. Detection system for droplet-based assays
EP2556170A4 (en) 2010-03-25 2014-01-01 Quantalife Inc Droplet transport system for detection
EP2566959A1 (en) 2010-04-30 2013-03-13 Roche Diagniostics GmbH System and method for purification and use of inorganic pyrophosphatase from aquifex aeolicus
US8530158B2 (en) 2010-05-10 2013-09-10 Life Technologies Corporation System and method for processing a biological sample
US20110287432A1 (en) 2010-05-21 2011-11-24 454 Life Science Corporation System and method for tailoring nucleotide concentration to enzymatic efficiencies in dna sequencing technologies
US9650629B2 (en) 2010-07-07 2017-05-16 Roche Molecular Systems, Inc. Clonal pre-amplification in emulsion
EP3100786A1 (en) 2010-07-22 2016-12-07 Gencell Biosystems Limited Composite liquid cells
AU2011305445B2 (en) 2010-09-24 2017-03-16 The Board Of Trustees Of The Leland Stanford Junior University Direct capture, amplification and sequencing of target DNA using immobilized primers
US8759038B2 (en) 2010-09-29 2014-06-24 Illumina Cambridge Limited Compositions and methods for sequencing nucleic acids
US20120077716A1 (en) 2010-09-29 2012-03-29 454 Life Sciences Corporation System and method for producing functionally distinct nucleic acid library ends through use of deoxyinosine
US9562897B2 (en) 2010-09-30 2017-02-07 Raindance Technologies, Inc. Sandwich assays in droplets
US20120322665A1 (en) 2010-10-08 2012-12-20 454 Life Sciences Corporation System and method for detection of hiv-1 clades and recombinants of the reverse transcriptase and protease regions
EP2633069B1 (en) 2010-10-26 2015-07-01 Illumina, Inc. Sequencing methods
CN103270174B (en) 2010-10-28 2016-08-24 生命技术公司 Chemical reinforcing type Primer composition, method and test kit
EP3574990B1 (en) 2010-11-01 2022-04-06 Bio-Rad Laboratories, Inc. System for forming emulsions
EP2643484A4 (en) 2010-11-22 2014-04-16 Univ California Methods of identifying a cellular nascent rna transcript
BR112013013003A2 (en) 2010-11-24 2016-08-09 Glaxo Group Ltd antigen binding protein and pharmaceutical composition
WO2012073045A2 (en) 2010-12-03 2012-06-07 Cyclogenix Ltd Polypeptide scaffold
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US9364803B2 (en) 2011-02-11 2016-06-14 Raindance Technologies, Inc. Methods for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
CN103534360A (en) 2011-03-18 2014-01-22 伯乐生命医学产品有限公司 Multiplexed digital assays with combinatorial use of signals
US20120244523A1 (en) 2011-03-25 2012-09-27 454 Life Sciences Corporation System and Method for Detection of HIV Integrase Variants
WO2012136792A2 (en) 2011-04-07 2012-10-11 Glaxo Group Limited Cck compositions
WO2012136790A1 (en) 2011-04-07 2012-10-11 Glaxo Group Limited Compositions comprising fusion proteins or conjugates with an improved half -life
JP2014512826A (en) 2011-04-25 2014-05-29 バイオ−ラド ラボラトリーズ インコーポレイテッド Methods and compositions for nucleic acid analysis
GB201108259D0 (en) * 2011-05-17 2011-06-29 Cambridge Entpr Ltd Gel beads in microfluidic droplets
WO2012162296A2 (en) 2011-05-23 2012-11-29 President And Fellows Of Harvard College Control of emulsions, including multiple emulsions
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
EP3709018A1 (en) 2011-06-02 2020-09-16 Bio-Rad Laboratories, Inc. Microfluidic apparatus for identifying components of a chemical reaction
FI3461807T3 (en) 2011-06-08 2023-09-07 Life Technologies Corp Design and development of novel detergents for use in pcr systems
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
WO2013006661A2 (en) 2011-07-06 2013-01-10 President And Fellows Of Harvard College Multiple emulsions and techniques for the formation of multiple emulsions
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
KR20130023091A (en) * 2011-08-26 2013-03-07 (주)바이오니아 Protein synthesis kit, protein expression and purification method using automatic purification apparatus
JP5924888B2 (en) * 2011-08-26 2016-05-25 関東化學株式会社 Nucleic acid extraction method, nucleic acid extraction reagent kit, and nucleic acid extraction reagent
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
DK3623481T3 (en) 2011-09-23 2021-11-15 Illumina Inc COMPOSITIONS FOR NUCLEIC ACID SEQUENCE
AU2012316218B2 (en) 2011-09-26 2016-03-17 Gen-Probe Incorporated Algorithms for sequence determinations
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
DE102011054101A1 (en) 2011-09-30 2013-04-04 Albert-Ludwigs-Universität Freiburg Method for the spatial arrangement of sample fragments for amplification and immobilization for further derivatizations
EP2768982A4 (en) 2011-10-21 2015-06-03 Adaptive Biotechnologies Corp Quantification of adaptive immune cell genomes in a complex mixture of cells
DE102011085473A1 (en) 2011-10-28 2013-05-02 Albert-Ludwigs-Universität Freiburg Method for the identification of aptamers
EP2776165A2 (en) 2011-11-07 2014-09-17 Illumina, Inc. Integrated sequencing apparatuses and methods of use
GB201120711D0 (en) 2011-12-01 2012-01-11 Univ Erasmus Medical Ct Method for classifying tumour cells
EP2788499B1 (en) 2011-12-09 2016-01-13 Illumina, Inc. Expanded radix for polymeric tags
AU2012347460B2 (en) 2011-12-09 2017-05-25 Adaptive Biotechnologies Corporation Diagnosis of lymphoid malignancies and minimal residual disease detection
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
WO2013101896A1 (en) 2011-12-30 2013-07-04 Quest Diagnostics Investments Incorporated Nucleic acid analysis using emulsion pcr
KR102028225B1 (en) 2012-01-25 2019-10-02 젠셀 바이오시스템즈 리미티드 Biomolecule isolation
US9701959B2 (en) 2012-02-02 2017-07-11 Invenra Inc. High throughput screen for biologically active polypeptides
US20130217023A1 (en) 2012-02-22 2013-08-22 454 Life Sciences Corporation System And Method For Generation And Use Of Compact Clonally Amplified Products
WO2013134162A2 (en) 2012-03-05 2013-09-12 Sequenta, Inc. Determining paired immune receptor chains from frequency matched subunits
NO2694769T3 (en) 2012-03-06 2018-03-03
US20130261984A1 (en) 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
WO2013155531A2 (en) 2012-04-13 2013-10-17 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
SG10201507700VA (en) 2012-05-08 2015-10-29 Adaptive Biotechnologies Corp Compositions and method for measuring and calibrating amplification bias in multiplexed pcr reactions
US10192024B2 (en) 2012-05-18 2019-01-29 454 Life Sciences Corporation System and method for generation and use of optimal nucleotide flow orders
EP2870264A4 (en) 2012-07-03 2016-03-02 Sloan Kettering Inst Cancer Quantitative assessment of human t-cell repertoire recovery after allogeneic hematopoietic stem cell transplantation
US9977861B2 (en) 2012-07-18 2018-05-22 Illumina Cambridge Limited Methods and systems for determining haplotypes and phasing of haplotypes
EP2882872B1 (en) 2012-08-13 2021-10-06 The Regents of The University of California Methods and systems for detecting biological components
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2881685C (en) 2012-08-14 2023-12-05 10X Genomics, Inc. Microcapsule compositions and methods
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
CN105189779B (en) 2012-10-01 2018-05-11 适应生物技术公司 The immunocompetence carried out by adaptive immunity receptor diversity and Clonal characterization is assessed
US10150996B2 (en) 2012-10-19 2018-12-11 Adaptive Biotechnologies Corp. Quantification of adaptive immune cell genomes in a complex mixture of cells
US9776182B2 (en) 2012-11-27 2017-10-03 Gencell Biosystems Ltd. Handling liquid samples
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2954065B1 (en) 2013-02-08 2021-07-28 10X Genomics, Inc. Partitioning and processing of analytes and other species
DK3327123T3 (en) * 2013-03-15 2019-11-25 Lineage Biosciences Inc METHODS FOR SEQUENCING THE IMMUN REPERTOIR
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
US9348897B2 (en) 2013-08-07 2016-05-24 Google Inc. Method and system for providing scheduling suggestions
EP2840148B1 (en) 2013-08-23 2019-04-03 F. Hoffmann-La Roche AG Methods for nucleic acid amplification
EP2848698A1 (en) 2013-08-26 2015-03-18 F. Hoffmann-La Roche AG System and method for automated nucleic acid amplification
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
EP3539944A1 (en) 2013-10-25 2019-09-18 Life Technologies Corporation Novel compounds for use in pcr systems and applications thereof
WO2015069634A1 (en) 2013-11-08 2015-05-14 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US10088973B2 (en) * 2013-11-08 2018-10-02 Google Llc Event scheduling presentation in a graphical user interface environment
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
US11193176B2 (en) 2013-12-31 2021-12-07 Bio-Rad Laboratories, Inc. Method for detecting and quantifying latent retroviral RNA species
EP3105326A4 (en) 2014-02-10 2018-01-10 Gencell Biosystems Limited Composite liquid cell (clc) mediated nucleic acid library preparation device, and methods for using the same
AU2015227054A1 (en) 2014-03-05 2016-09-22 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
AU2015243445B2 (en) 2014-04-10 2020-05-28 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
CN106795553B (en) 2014-06-26 2021-06-04 10X基因组学有限公司 Methods of analyzing nucleic acids from individual cells or cell populations
CN106575322B (en) 2014-06-26 2019-06-18 10X基因组学有限公司 The method and system of nucleic acid sequence assembly
US10697007B2 (en) 2014-06-27 2020-06-30 The Regents Of The University Of California PCR-activated sorting (PAS)
AU2015318017B2 (en) 2014-09-18 2022-02-03 Illumina, Inc. Methods and systems for analyzing nucleic acid sequencing data
WO2016053883A1 (en) 2014-10-03 2016-04-07 Life Technologies Corporation Detection, identification, validation and enrichment of target nucleic acids
EP3209419A4 (en) 2014-10-22 2018-10-03 The Regents of The University of California High definition microdroplet printer
AU2015339191A1 (en) 2014-10-29 2017-05-18 Adaptive Biotechnologies Corp. Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
KR20170073667A (en) 2014-10-29 2017-06-28 10엑스 제노믹스, 인크. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
EP3498866A1 (en) 2014-11-25 2019-06-19 Adaptive Biotechnologies Corp. Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
CN112126675B (en) 2015-01-12 2022-09-09 10X基因组学有限公司 Method and system for preparing nucleic acid sequencing library and library prepared by using same
CN107209814B (en) 2015-01-13 2021-10-15 10X基因组学有限公司 System and method for visualizing structural variation and phase information
AU2016215304B2 (en) 2015-02-04 2022-01-27 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
CA2975529A1 (en) 2015-02-09 2016-08-18 10X Genomics, Inc. Systems and methods for determining structural variation and phasing using variant call data
EP3262188B1 (en) 2015-02-24 2021-05-05 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
US11047008B2 (en) 2015-02-24 2021-06-29 Adaptive Biotechnologies Corporation Methods for diagnosing infectious disease and determining HLA status using immune repertoire sequencing
DK3271477T3 (en) 2015-03-20 2020-09-14 Novozymes As Drop-based selection by injection
EP4089398A1 (en) 2015-03-24 2022-11-16 Illumina, Inc. Carrier assemblies and systems for imaging samples for biological or chemical analysis
EP3277294A4 (en) 2015-04-01 2018-11-14 Adaptive Biotechnologies Corp. Method of identifying human compatible t cell receptors specific for an antigenic target
WO2016164596A2 (en) 2015-04-07 2016-10-13 Novozymes A/S Methods for selecting enzymes having lipase activity
CN107667177A (en) * 2015-04-07 2018-02-06 诺维信公司 Method for selecting the enzyme with enhancing activity
CN107849600A (en) 2015-06-09 2018-03-27 生命技术公司 For the method for molecular labeling, system, composition, kit, device and computer-readable media
WO2017022696A1 (en) 2015-07-31 2017-02-09 国立研究開発法人理化学研究所 Method of manufacturing membrane protein and utilization thereof
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
CN108289797B (en) 2015-10-13 2022-01-28 哈佛学院院长及董事 Systems and methods for making and using gel microspheres
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
US10774370B2 (en) 2015-12-04 2020-09-15 10X Genomics, Inc. Methods and compositions for nucleic acid analysis
SG11201806757XA (en) 2016-02-11 2018-09-27 10X Genomics Inc Systems, methods, and media for de novo assembly of whole genome sequence data
CN109312333A (en) 2016-04-07 2019-02-05 诺维信公司 The method for selecting that there is the enzyme of proteinase activity
US10287620B2 (en) * 2016-04-29 2019-05-14 Bio-Rad Laboratories, Inc. Digital polymerase fidelity assay
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US11142791B2 (en) 2016-08-10 2021-10-12 The Regents Of The University Of California Combined multiple-displacement amplification and PCR in an emulsion microdroplet
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
WO2018064116A1 (en) 2016-09-28 2018-04-05 Illumina, Inc. Methods and systems for data compression
IL290157B2 (en) 2016-10-14 2023-09-01 Illumina Inc Cartridge assembly
EP3555288B1 (en) 2016-12-19 2021-02-03 Editas Medicine, Inc. Emulsion-based screening methods
CA3047328A1 (en) 2016-12-21 2018-06-28 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
GB201707143D0 (en) 2017-05-04 2017-06-21 Plasticell Ltd Method for producing cells
EP3625715A4 (en) 2017-05-19 2021-03-17 10X Genomics, Inc. Systems and methods for analyzing datasets
EP4230746A3 (en) 2017-05-26 2023-11-01 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019076955A1 (en) 2017-10-18 2019-04-25 Novozymes A/S Immunoassay for bio-active polypeptide stability
US10501739B2 (en) 2017-10-18 2019-12-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
US11634757B2 (en) 2017-10-20 2023-04-25 Stilla Technologies Emulsions with improved stability
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Methods and systems for nuclecic acid preparation and chromatin analysis
EP4241882A3 (en) 2017-10-27 2023-12-06 10X Genomics, Inc. Methods for sample preparation and analysis
EP3954782A1 (en) 2017-11-15 2022-02-16 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
EP3740591A4 (en) 2018-01-19 2022-02-23 Bio-Rad Laboratories, Inc. Mutant dna polymerases
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
CN112262218A (en) 2018-04-06 2021-01-22 10X基因组学有限公司 System and method for quality control in single cell processing
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
CN113423841A (en) 2018-12-13 2021-09-21 Dna斯克瑞普特公司 Direct oligonucleotide synthesis on cells and biomolecules
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
SG11202108788TA (en) 2019-02-12 2021-09-29 10X Genomics Inc Methods for processing nucleic acid molecules
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
US11920183B2 (en) 2019-03-11 2024-03-05 10X Genomics, Inc. Systems and methods for processing optically tagged beads
CA3138806A1 (en) 2019-05-22 2020-11-26 Dalia Dhingra Method and apparatus for simultaneous targeted sequencing of dna, rna and protein
US11667954B2 (en) 2019-07-01 2023-06-06 Mission Bio, Inc. Method and apparatus to normalize quantitative readouts in single-cell experiments
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
CN116997411A (en) 2021-01-13 2023-11-03 加利福尼亚太平洋生物科学股份有限公司 Surface structuring using colloid assembly
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
WO2023122363A1 (en) 2021-12-23 2023-06-29 Illumina Software, Inc. Dynamic graphical status summaries for nucelotide sequencing
WO2023129764A1 (en) 2021-12-29 2023-07-06 Illumina Software, Inc. Automatically switching variant analysis model versions for genomic analysis applications
WO2023225488A1 (en) 2022-05-16 2023-11-23 Enkefalos Biosciences, Inc. Blood-brain barrier translocating peptides and related molecules and methods of use thereof
WO2024072614A1 (en) 2022-09-27 2024-04-04 Nautilus Subsidiary, Inc. Polypeptide capture, in situ fragmentation and identification

Family Cites Families (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2747570A (en) * 1954-05-19 1956-05-29 Jobst Conrad Means for assisting return circulation of fluids in an animal body
US3826249A (en) * 1973-01-30 1974-07-30 A Lee Leg constricting apparatus
US3908642A (en) * 1973-10-29 1975-09-30 Pred Vinmont Means for aerating and applying air pulsations within casts
US4206751A (en) * 1978-03-31 1980-06-10 Minnesota Mining And Manufacturing Company Intermittent compression device
US4801529A (en) * 1985-06-18 1989-01-31 Brandeis University Methods for isolating mutant microoganisms using microcapsules coated with indicator material
US5099030A (en) * 1987-03-09 1992-03-24 The Procter & Gamble Company Novel compounds, pharmaceutical compositions, and methods for treating inflammation and pain
EP0494955B1 (en) * 1989-10-05 1998-07-15 Optein, Inc. Cell-free synthesis and isolation of novel genes and polypeptides
JP3087907B2 (en) * 1990-02-16 2000-09-18 エフ．ホフマン ― ラ ロシュ アーゲー Improvement of specificity and simplicity of polymerase chain reaction
ES2030639T1 (en) 1990-09-21 1992-11-16 Bioplex Medical B.V. DEVICE FOR PLACING STYPTIC MATERIAL, IN CONCRETE IN A FIBROUS OR SPONJIFORM FORM ON PERFORATED BLOOD VESSELS, IN CONCRETE ARTERIES.
GB9107628D0 (en) 1991-04-10 1991-05-29 Moonbrook Limited Preparation of diagnostic agents
US5955341A (en) * 1991-04-10 1999-09-21 The Scripps Research Institute Heterodimeric receptor libraries using phagemids
EP0597960B1 (en) * 1991-08-10 1999-01-20 Medical Research Council Treatment of cell populations
WO1993008472A1 (en) 1991-10-15 1993-04-29 Multilyte Limited Binding assay employing labelled reagent
US5270170A (en) * 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
US5413924A (en) 1992-02-13 1995-05-09 Kosak; Kenneth M. Preparation of wax beads containing a reagent for release by heating
DE4223169C1 (en) 1992-07-10 1993-11-25 Ferring Arzneimittel Gmbh Process for the microencapsulation of water-soluble active substances
RU2048522C1 (en) * 1992-10-14 1995-11-20 Институт белка РАН Method of nucleic acid copying, method of their expression and a medium for their realization
IL104384A (en) * 1993-01-13 1996-11-14 Yeda Res & Dev Method for screening catalytic non-enzyme polypeptides and proteins
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
CA2160878A1 (en) 1993-04-19 1994-10-27 Sandra Gertrude Mcelligott Encapsulation of nucleic acids with conjugates that facilitate and target cellular uptake and gene expression
WO1994024314A1 (en) * 1993-04-19 1994-10-27 Kauffman Stuart A Random chemistry for the generation of new compounds
AU8124694A (en) * 1993-10-29 1995-05-22 Affymax Technologies N.V. In vitro peptide and antibody display libraries
DE4402038A1 (en) 1994-01-25 1995-07-27 Borries Horst Von Blister pack
EP0804249A2 (en) 1994-03-15 1997-11-05 Brown University Research Foundation Polymeric gene delivery system
US5508348A (en) * 1994-08-12 1996-04-16 The Research Foundation Of Suny Inverted emulsion pathways to conductive polymers
US5680283A (en) 1994-09-30 1997-10-21 Kabushiki Kaisha Toshiba Magnetic head and magnetic disk drive
EP0822990A4 (en) 1995-04-24 2002-07-03 Chromaxome Corp Methods for generating and screening novel metabolic pathways
US5910408A (en) * 1995-06-07 1999-06-08 The General Hospital Corporation Catalytic DNA having ligase activity
US5756122A (en) 1995-06-07 1998-05-26 Georgetown University Liposomally encapsulated nucleic acids having high entrapment efficiencies, method of manufacturer and use thereof for transfection of targeted cells
US5989892A (en) 1995-06-14 1999-11-23 Tonen Corporation Microorganisms, demulsifiers and processes for breaking an emulsion
AU6691496A (en) 1995-08-01 1997-02-26 Advanced Therapies, Inc. Enhanced artificial viral envelopes for cellular delivery of therapeutic substances
DE19646372C1 (en) * 1995-11-11 1997-06-19 Evotec Biosystems Gmbh Conjugates of polypeptide and encoding nucleic acid
GB9608540D0 (en) 1996-04-25 1996-07-03 Medical Res Council Isolation of enzymes
US5876711A (en) * 1996-05-31 1999-03-02 Onyx Pharmaceuticals, Inc. Methods and compositions for determining the tumor suppressor status of cells
US6083693A (en) 1996-06-14 2000-07-04 Curagen Corporation Identification and comparison of protein-protein interactions that occur in populations
US5876771A (en) 1996-06-20 1999-03-02 Tetra Laval Holdings & Finance, Sa Process and article for determining the residence time of a food particle
CA2236867A1 (en) 1996-09-27 1998-04-02 Icos Corporation Method to identify compounds for disrupting protein/protein interactions
US6395524B2 (en) 1996-11-27 2002-05-28 University Of Washington Thermostable polymerases having altered fidelity and method of identifying and using same
US6310354B1 (en) 1996-12-03 2001-10-30 Erkki Soini Method and a device for monitoring nucleic acid amplification reactions
CN1238366C (en) 1997-01-21 2006-01-25 综合医院公司 Selection of proteins using RNA-protein fusions
CA2196496A1 (en) 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
GB9703369D0 (en) * 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
EP0975969A4 (en) 1997-03-18 2002-07-31 Chromaxome Corp Methods for screening compounds using encapsulated cells
US6048551A (en) * 1997-03-27 2000-04-11 Hilfinger; John M. Microsphere encapsulation of gene transfer vectors
CA2792122C (en) 1997-07-07 2015-09-08 Medical Research Council In vitro sorting method
JP2981547B1 (en) 1998-07-02 1999-11-22 農林水産省食品総合研究所長 Cross-flow type microchannel device and method for producing or separating emulsion using the device
EP1100889A4 (en) 1998-07-17 2002-03-06 Mirus Corp Micellar systems
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
GB9921155D0 (en) 1999-09-08 1999-11-10 Medical Res Council Selection system
CN1189159C (en) 2000-05-05 2005-02-16 欧莱雅 Micro-capsule contg. water soluble beauty-care activity component water nuclear, and composition contg. same
GB0022458D0 (en) 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
US6936264B2 (en) 2001-03-05 2005-08-30 The Procter & Gamble Company Delivery of reactive agents via multiple emulsions for use in shelf stable products
JP3570714B2 (en) 2001-05-24 2004-09-29 株式会社リコー Developer container and image forming apparatus
GB0114856D0 (en) 2001-06-18 2001-08-08 Medical Res Council Selection by avidity capture
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
GB0221053D0 (en) * 2002-09-11 2002-10-23 Medical Res Council Single-molecule in vitro evolution
JP4395133B2 (en) 2002-12-20 2010-01-06 カリパー・ライフ・サイエンシズ・インク． Single molecule amplification and detection of DNA
AU2004254552B2 (en) 2003-01-29 2008-04-24 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids

Also Published As

Publication number Publication date
ATE278013T1 (en) 2004-10-15
EP1496120A3 (en) 2005-05-11
US6489103B1 (en) 2002-12-03
CA2792122A1 (en) 1999-01-21
ES2294427T3 (en) 2008-04-01
JP4156009B2 (en) 2008-09-24
EP2258846A3 (en) 2012-03-21
EP1482036B1 (en) 2007-10-03
US7252943B2 (en) 2007-08-07
ATE374815T1 (en) 2007-10-15
DE69826697T2 (en) 2006-02-16
EP1801214A3 (en) 2009-05-20
US20130190189A1 (en) 2013-07-25
DK1496120T3 (en) 2007-07-30
JP2005192572A (en) 2005-07-21
EP1496120B1 (en) 2007-03-28
AU8123198A (en) 1999-02-08
EP1482036A3 (en) 2004-12-08
CA2584453A1 (en) 1999-01-21
ES2230701T3 (en) 2005-05-01
US20070259374A1 (en) 2007-11-08
CA2792122C (en) 2015-09-08
EP1482036A2 (en) 2004-12-01
EP1801214B1 (en) 2010-11-10
PT1496120E (en) 2007-04-30
CY1111537T1 (en) 2015-08-05
JP2001509380A (en) 2001-07-24
PT1801214E (en) 2011-01-20
WO1999002671A1 (en) 1999-01-21
AU736836B2 (en) 2001-08-02
US7582446B2 (en) 2009-09-01
DE69837453T2 (en) 2007-12-20
EP1019496A1 (en) 2000-07-19
US20030124586A1 (en) 2003-07-03
ES2283936T3 (en) 2007-11-01
ES2402516T3 (en) 2013-05-06
DE69841997D1 (en) 2010-12-23
DK1019496T3 (en) 2005-01-10
GB2342094B (en) 2002-10-23
GB2342094A (en) 2000-04-05
US20070077579A1 (en) 2007-04-05
ATE487790T1 (en) 2010-11-15
US7638276B2 (en) 2009-12-29
US20050042648A1 (en) 2005-02-24
EP1496120A2 (en) 2005-01-12
ATE358177T1 (en) 2007-04-15
JP4299255B2 (en) 2009-07-22
EP1801214A2 (en) 2007-06-27
JP2007143563A (en) 2007-06-14
EP2258846A2 (en) 2010-12-08
US9528106B2 (en) 2016-12-27
EP1908832A3 (en) 2009-05-20
DE69838521T2 (en) 2008-05-21
US20050069920A1 (en) 2005-03-31
EP1908832A2 (en) 2008-04-09
DE69838521D1 (en) 2007-11-15
US8367326B2 (en) 2013-02-05
EP1019496B1 (en) 2004-09-29
DE69826697D1 (en) 2004-11-04
CA2295324C (en) 2012-12-18
DE69837453D1 (en) 2007-05-10
EP1908832B1 (en) 2012-12-26
JP4298912B2 (en) 2009-07-22
GB9929447D0 (en) 2000-02-09
DK1801214T3 (en) 2011-01-24
US7138233B2 (en) 2006-11-21

Similar Documents

Publication Publication Date Title
CA2295324A1 (en) In vitro sorting method
CA2357037A1 (en) Optical sorting method
CA2467215A1 (en) Emulsion compositions
Tian et al. Artificial morphogen-mediated differentiation in synthetic protocells
DE60120713T2 (en) FUNCTIONAL PROTEIN ARRAY
US20050202429A1 (en) Microcapsules with controlable permeability encapsulating a nucleic acid amplification reaction mixture and their use as reaction compartment for parallels reactions
Blaser Heterogeneous catalysis for fine chemicals production
WO2004024917A3 (en) Single-molecule in vitro evolution
BR9508170A (en) Process for the separation of a solubilized organic rhodium-organophosphite complex catalyst and free organophosphite binder from a non-aqueous hydroformylation reaction mixture
DE69217497D1 (en) METHOD FOR SYNTHESISING THE DIFFERENT COLLECTIONS OF OLIGOMERS
WO2006038035A3 (en) In vitro evolution in microfluidic systems
RU92000528A (en) METHOD OF REPRODUCTION, EXPRESSION, DIAGNOSTICS AND CLONING OF NUCLEIC ACIDS IN IMMOBILIZED MEDIA
WO2002066613A3 (en) Novel ecdysone receptor/invertebrate retinoid x receptor-based inducible gene expression system
JP2011092218A5 (en)
Peng et al. 3D‐printed phenacrylate decarboxylase flow reactors for the chemoenzymatic synthesis of 4‐hydroxystilbene
DE3860663D1 (en) METHOD FOR PRODUCING MICROCAPSULES, THE MICROCAPSULES THEREFORE AVAILABLE AND THE USE THEREOF.
EP1436609B1 (en) Microfluidic extraction method
Galinis et al. DNA nanoparticles for improved protein synthesis in vitro
DE69813848D1 (en) Process for the lysis of microorganisms
Okano et al. Cell-free protein synthesis in a microchamber revealed the presence of an optimum compartment volume for high-order reactions
Alex Wong et al. A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalized Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution
AU2986395A (en) Method for screening compound libraries
Jesorka et al. Neither shaken nor stirred
ATE245034T1 (en) MULTI-STEP PROCESS FOR PRODUCING GAS-FILLED MICRO CAPSULES
ATE153997T1 (en) DECOLORING OF ALKYLATED DIARYLAMINES

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20180629