CA2303891A1 - Assay sonication apparatus and methodology - Google Patents

Assay sonication apparatus and methodology Download PDF

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Publication number
CA2303891A1
CA2303891A1 CA002303891A CA2303891A CA2303891A1 CA 2303891 A1 CA2303891 A1 CA 2303891A1 CA 002303891 A CA002303891 A CA 002303891A CA 2303891 A CA2303891 A CA 2303891A CA 2303891 A1 CA2303891 A1 CA 2303891A1
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CA
Canada
Prior art keywords
cell
sonicating
recited
binding
assay
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002303891A
Other languages
French (fr)
Other versions
CA2303891C (en
Inventor
Jacob N. Wohlstadter
James Wilbur
George Sigal
Mark Martin
Alan Fischer
Larry R. Helms
Ramin Darvari
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Meso Scale Technologies LLC
Original Assignee
Meso Scale Technologies, Llc.
Jacob N. Wohlstadter
James Wilbur
George Sigal
Mark Martin
Alan Fischer
Larry R. Helms
Ramin Darvari
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Meso Scale Technologies, Llc., Jacob N. Wohlstadter, James Wilbur, George Sigal, Mark Martin, Alan Fischer, Larry R. Helms, Ramin Darvari filed Critical Meso Scale Technologies, Llc.
Publication of CA2303891A1 publication Critical patent/CA2303891A1/en
Application granted granted Critical
Publication of CA2303891C publication Critical patent/CA2303891C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/808—Optical sensing apparatus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property

Abstract

An assay apparatus comprising a cell (10) with a working electrode and a sonicating device (16) structurally coupled to the cell for sonicating the contents of the cell.

Claims (45)

1. An apparatus for use in conducting an electrochemiluminescence binding assay, comprising:
(a) a cell; and (b) means for sonicating contents of said cell.
2. An apparatus as recited in claim 1, wherein said sonicating means is capable of providing sonication energy at from 0.1 to 5kHz.
3. An apparatus as recited in claim 1, wherein said sonicating means is capable of providing sonication energy at more than 1,000, kHz.
4. An apparatus as recited in claim 1, wherein said sonicating means has a power of from 0.001 to 10 watts.
5. An apparatus for use in carrying out a binding assay, comprising:
(a) a cell; and (b) means, structurally coupled to said cell, for sonicating contents of said cell.
6. An apparatus as recited in claim 5, wherein said sonicating means is capable of providing sonication energy at from 0.1 to 10,000kHz.
7. An apparatus as recited in claim 5, wherein said sonicating means has a power of from 0.001 to 10 watts.
8. An apparatus as recited in claim 5, wherein said assay is an electrochemiluminescence assay.
9. An apparatus for use in conducting an electrochemiluminescence binding assay, comprising:
(a) a cell; and (b) means, in solid contact with said cell, for sonicating contents of said cell.
10. An apparatus as recited in claim 9, wherein said sonicating means is capable of providing sonication energy at from 0.1 to 10,000kHz.
11. An apparatus as recited in claim 9, wherein said sonicating means has a power of from 0.001 to 10 watts.
12. An apparatus as recited in claim 9, wherein said apparatus is portable.
13. An apparatus for use in conducting an electrochemiluminescence binding assay, comprising:
(a) a cell including a working electrode;
and (b) means, structurally coupled to said cell, for sonicating contents of said cell.
14. An apparatus as recited in claim 13, wherein said sonicating means is capable of providing sonication energy at from 0.1 to 10,000kHz.
15. An apparatus as recited in claim 13, wherein said sonicating means has a power of from 0.001 to 10 watts.
16. An apparatus as recited in claim 13, wherein said apparatus is portable.
17. The apparatus according to claim 13, wherein said sonicating means is structurally coupled to said working electrode.
18. The apparatus according to claim 13, wherein said sonicating means comprises a piezoelectric device.
19. The apparatus according to claim 13 further comprising a power supply coupled to said working electrode, for supplying electrical energy to said electrode.
20. The apparatus according to claim 13 further comprising means for detecting luminescence from said cell.
21. The apparatus according to claim 13, wherein a binding reaction occurs at said working electrode.

43~
22. An apparatus as recited in claim 13, wherein said apparatus is for the conduct of an immunoassay a nucleic acid hybridization assay or a receptor ligand binding assay.
23. An apparatus for use in carrying out electrochemiluminescence measurements, comprising:
(a) a cell;
(b) one or more electrodes each of said one or more electrodes having one or a plurality of binding domains, each of said domains containing a reagent capable of binding a component of a binding electrochemiluminescence assay; and (c) means, structurally coupled to said cell, for sonicating contents of said cell.
24. An apparatus as recited in claim 23, wherein said sonicating means is capable of providing sonication energy at from 0.1 to 10,000kHz.
25. An apparatus as recited in claim 23, wherein said sonicating means has a power of from 0.001 to to watts.
26. An apparatus as recited in claim 23, wherein said apparatus is portable.
27. The apparatus according to claim 23, wherein said sonicating means is structurally coupled to said one or more electrodes.
28. The apparatus according to claim 23, wherein said sonicating means comprises a piezoelectric device.
29. The apparatus according to claim 23 further comprising a power supply coupled to said one or more electrodes, for supplying electrical energy to said electrode.
30. The apparatus according to claim 23 further comprising means for detecting luminescence from said cell.
31. The apparatus according to claim 23, wherein a binding reaction occurs at said one or more electrodes.
32. An apparatus as recited in claim 23 wherein said reagent is selected from the group consisting of antibodies, antibody fragments, enzymes, nucleic acids and receptors.
33. An apparatus for use in carrying out a binding assay, comprising:
(a) a cell including a diaphragm; and (b) means structurally coupled through said diaphragm to said cell for sonicating contents thereof.
34. An apparatus as recited in claim 33, wherein said cell includes a solid phase support for conduct of a binding reaction.
35. An apparatus as recited in claim 33, wherein said apparatus includes a working electrode for the conduct of an electrochemiluminescence assay.
36. An apparatus as recited in claim 33, wherein said sonication means is a piezoelectric device.
37. An assay system for conducting a binding assay for the detection or quantitation of an analyte comprising:
(a) a cartridge comprising a cell;
(b) a cartridge receptacle including means for structurally coupling to said cell in said cartridge;
(c) means for sonicating the contents of said cell; and (d) means for selectively detecting or quantitating light from each of said binding domains.
38. An assay system as recited in claim 37, wherein said cartridge further includes one or more electrodes each of said one or more electrodes having one or a plurality of binding domains, each of said domains containing a reagent capable of binding a component of a binding electrochemiluminescence assay.
39. An assay system as recited in claim 37, wherein said cell further includes a diaphragm
40. An assay system as recited in claim 37, wherein said means for sonicating the contents of said cell includes a piezoelectric device.
41. A method for conducting an electrochemiluminescence binding assay comprising the steps of:
(a) introducing a composition containing an electrochemiluminescent moiety into a cell including a working electrode;
(b) sonicating said composition in said cell with means structurally coupled to said cell;
(c) applying electrical energy to said electrode to cause said electrochemiluminescent moiety to luminesce; and (d) detecting or quantitating the electrochemiluminescence from said cell.
42. A method as recited in claim 41, wherein said sonicating means is a piezoelectric device.
43. A method as recited in claim 41, for cleaning debris in said cell.
44. A method for conducting an electrochemiluminescence binding assay comprising the steps of:
(a) introducing a composition containing an electrochemiluminescent moiety into a cell including one or more electrodes each comprising a composite containing a matrix and a multiplicity of carbon particles dispersed therein, each of said one or more electrodes having one or a plurality of binding domains, each of said domains containing a reagent capable of binding a component of a binding electrochemiluminescence assay;
(b) sonicating said composition in said cell with means structurally coupled to said cell;
(c) applying electrical energy to said electrode to cause a said electrochemiluminescent moiety bound to said one or a plurality of binding domains to luminesce; and (d) detecting or quantitating the electrochemiluminescence from said cell.
45. A method for conducting a kinetic binding assay by electrochemiluminescence comprising the steps of:
(a) introducing a composition containing an electrochemiluminescent moiety into a cell including one or more electrodes each of said one or more electrodes having one or a plurality of binding domains, each of said domains containing a reagent capable of binding a component of a binding electrochemiluminescence assay;
(b) sonicating said composition in said cell with means structurally coupled to said cell;
(c) applying electrical energy to said electrode to cause a said electrochemiluminescent moiety bound to said one or a plurality of binding domains to luminesce so as to carry out a kinetic assay; and (d) detecting or quantitating the electrochemiluminescence from said cell.
CA002303891A 1997-09-18 1998-09-11 Assay sonication apparatus and methodology Expired - Fee Related CA2303891C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/932,985 1997-09-18
US08/932,985 US6413783B1 (en) 1997-09-18 1997-09-18 Assay sonication apparatus and methodology
PCT/US1998/019118 WO1999014599A1 (en) 1997-09-18 1998-09-11 Assay sonication apparatus and methodology

Publications (2)

Publication Number Publication Date
CA2303891A1 true CA2303891A1 (en) 1999-03-25
CA2303891C CA2303891C (en) 2009-11-17

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA002303891A Expired - Fee Related CA2303891C (en) 1997-09-18 1998-09-11 Assay sonication apparatus and methodology

Country Status (7)

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US (2) US6413783B1 (en)
EP (1) EP1018015B1 (en)
AT (1) ATE405835T1 (en)
AU (1) AU9774598A (en)
CA (1) CA2303891C (en)
DE (1) DE69839911D1 (en)
WO (1) WO1999014599A1 (en)

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Publication number Publication date
CA2303891C (en) 2009-11-17
AU9774598A (en) 1999-04-05
EP1018015B1 (en) 2008-08-20
EP1018015A4 (en) 2005-10-19
US7008796B2 (en) 2006-03-07
EP1018015A1 (en) 2000-07-12
US20020137234A1 (en) 2002-09-26
DE69839911D1 (en) 2008-10-02
ATE405835T1 (en) 2008-09-15
US6413783B1 (en) 2002-07-02
WO1999014599A1 (en) 1999-03-25

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