CA2319437A1 - Methods and reagents for decreasing allergic reactions - Google Patents

Methods and reagents for decreasing allergic reactions Download PDF

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Publication number
CA2319437A1
CA2319437A1 CA002319437A CA2319437A CA2319437A1 CA 2319437 A1 CA2319437 A1 CA 2319437A1 CA 002319437 A CA002319437 A CA 002319437A CA 2319437 A CA2319437 A CA 2319437A CA 2319437 A1 CA2319437 A1 CA 2319437A1
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CA
Canada
Prior art keywords
ige
binding
binding sites
ige binding
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002319437A
Other languages
French (fr)
Other versions
CA2319437C (en
Inventor
Gary A. Bannon
A. Wesley Burks, Jr.
Hugh A. Sampson
Gael Cockrell
Ricki M. Helm
Nina E. King
J. Steven Stanley
David S. Shin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Arkansas
Icahn School of Medicine at Mount Sinai
Original Assignee
Mount Sinai School of Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mount Sinai School of Medicine filed Critical Mount Sinai School of Medicine
Publication of CA2319437A1 publication Critical patent/CA2319437A1/en
Application granted granted Critical
Publication of CA2319437C publication Critical patent/CA2319437C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/806—Antigenic peptides or proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/868—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof involving autoimmunity, allergy, immediate hypersensitivity, delayed hypersensitivity, immunosuppression, or immunotolerance

Abstract

It has been determined that allergens, which are characterized by both humoral (IgE) and cellular (T cell) binding sites, can be modified to be less allergenic by modifying the IgE binding sites. The IgE
binding sites can be converted to non-IgE binding sites by masking the site with a compound that prevents IgE binding or by altering as little as a single amino acid within the protein, most typically a hydrophobic residue towards the center of the IgE-binding epitope, to eliminate IgE binding. The method allows the protein to be altered as minimally as possible, other than within the IgE-binding sites, while retaining the ability of the protein to activate T cells, and, in some embodiments by not significantly altering or decreasing IgG binding capacity. The examples use peanut allergens to demonstrate alteration of IgE binding sites. The critical amino acids within each of the IgE binding epitopes of the peanut protein that are important to immunoglobulin binding have been determined. Substitution of even a single amino acid within each of the epitopes led to loss of IgE
binding. Although the epitopes shared no common amino acid sequence motif, the hydrophobic residues located in the center of the epitope appeared to be most critical to IgE binding.
CA002319437A 1998-01-31 1999-01-29 Methods and reagents for decreasing allergic reactions Expired - Fee Related CA2319437C (en)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US7328398P 1998-01-31 1998-01-31
US60/073,283 1998-01-31
US7463398P 1998-02-13 1998-02-13
US7459098P 1998-02-13 1998-02-13
US7462498P 1998-02-13 1998-02-13
US60/074,590 1998-02-13
US60/074,624 1998-02-13
US60/074,633 1998-02-13
US14122098A 1998-08-27 1998-08-27
US09/141,220 1998-08-27
PCT/US1999/002031 WO1999038978A1 (en) 1998-01-31 1999-01-29 Methods and reagents for decreasing allergic reactions

Publications (2)

Publication Number Publication Date
CA2319437A1 true CA2319437A1 (en) 1999-08-05
CA2319437C CA2319437C (en) 2009-06-16

Family

ID=27535962

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002319437A Expired - Fee Related CA2319437C (en) 1998-01-31 1999-01-29 Methods and reagents for decreasing allergic reactions

Country Status (8)

Country Link
US (1) US7485708B2 (en)
EP (1) EP1051494B1 (en)
JP (1) JP2002501748A (en)
AT (1) ATE430199T1 (en)
AU (1) AU743647B2 (en)
CA (1) CA2319437C (en)
DE (1) DE69940805D1 (en)
WO (1) WO1999038978A1 (en)

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Also Published As

Publication number Publication date
EP1051494A1 (en) 2000-11-15
EP1051494B1 (en) 2009-04-29
AU743647B2 (en) 2002-01-31
WO1999038978A1 (en) 1999-08-05
JP2002501748A (en) 2002-01-22
DE69940805D1 (en) 2009-06-10
AU2350599A (en) 1999-08-16
US20030049237A1 (en) 2003-03-13
ATE430199T1 (en) 2009-05-15
US7485708B2 (en) 2009-02-03
CA2319437C (en) 2009-06-16

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