CA2319777A1 - Dideoxy dye terminators - Google Patents

Dideoxy dye terminators Download PDF

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Publication number
CA2319777A1
CA2319777A1 CA002319777A CA2319777A CA2319777A1 CA 2319777 A1 CA2319777 A1 CA 2319777A1 CA 002319777 A CA002319777 A CA 002319777A CA 2319777 A CA2319777 A CA 2319777A CA 2319777 A1 CA2319777 A1 CA 2319777A1
Authority
CA
Canada
Prior art keywords
dye terminators
dideoxy dye
dideoxy
dye
dye terminator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002319777A
Other languages
French (fr)
Other versions
CA2319777C (en
Inventor
Shiv Kumar
Satyam Nampalli
Bernard F. Mcardle
Carl W. Fuller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions USA LLC
Original Assignee
Amersham Pharmacia Biotech Inc.
Shiv Kumar
Satyam Nampalli
Bernard F. Mcardle
Carl W. Fuller
Amersham Biosciences Corp
Ge Healthcare Bio-Sciences Corp.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Pharmacia Biotech Inc., Shiv Kumar, Satyam Nampalli, Bernard F. Mcardle, Carl W. Fuller, Amersham Biosciences Corp, Ge Healthcare Bio-Sciences Corp. filed Critical Amersham Pharmacia Biotech Inc.
Publication of CA2319777A1 publication Critical patent/CA2319777A1/en
Application granted granted Critical
Publication of CA2319777C publication Critical patent/CA2319777C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Abstract

A kit for DNA sequencing comprising a first, second, third and fourth dye terminator molecules, each of the dye terminator molecules comprising a dye molecule, a linker of at least 10 atoms in length and either ddATP, ddCTP, ddGTP or ddTTP as a mono or tri-phosphate and a thermostable DNA polymerase.
CA002319777A 1998-02-04 1999-02-02 Dideoxy dye terminators Expired - Fee Related CA2319777C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US1869598A 1998-02-04 1998-02-04
US09/018,695 1998-02-04
PCT/US1999/002104 WO1999040223A1 (en) 1998-02-04 1999-02-02 Dideoxy dye terminators

Publications (2)

Publication Number Publication Date
CA2319777A1 true CA2319777A1 (en) 1999-08-12
CA2319777C CA2319777C (en) 2008-01-08

Family

ID=21789323

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002319777A Expired - Fee Related CA2319777C (en) 1998-02-04 1999-02-02 Dideoxy dye terminators

Country Status (8)

Country Link
US (1) US6949635B1 (en)
EP (1) EP1060264B1 (en)
JP (1) JP4306960B2 (en)
AT (1) ATE299190T1 (en)
AU (1) AU2571799A (en)
CA (1) CA2319777C (en)
DE (1) DE69926057T2 (en)
WO (1) WO1999040223A1 (en)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4306960B2 (en) * 1998-02-04 2009-08-05 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション Dideoxy dye terminator
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6358684B1 (en) * 1999-08-27 2002-03-19 Pe Corporation UV excitable fluorescent energy transfer dyes
CA2382063A1 (en) * 1999-09-17 2001-03-22 Shiv Kumar Charge-modified nucleic acid terminators
AU2002213125B2 (en) 2000-10-11 2007-11-22 Applied Biosystems, Llc. Fluorescent nucleobase conjugates having anionic linkers
JP4676654B2 (en) * 2001-07-19 2011-04-27 富士フイルム株式会社 Fluorescent nucleotide and labeling method using the same
JP2007524833A (en) * 2003-07-01 2007-08-30 ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン Method and apparatus for diagnosing bone tissue condition
US20050119587A1 (en) * 2003-07-01 2005-06-02 University Of Michigan Method and apparatus for evaluating connective tissue conditions
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP2248911A1 (en) 2004-02-19 2010-11-10 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US8227621B2 (en) * 2005-06-30 2012-07-24 Li-Cor, Inc. Cyanine dyes and methods of use
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7729749B2 (en) * 2005-09-01 2010-06-01 The Regents Of The University Of Michigan Method and apparatus for evaluating connective tissue conditions
JP2009508571A (en) * 2005-09-16 2009-03-05 ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン Method and system for measuring composition directly under surface of specimen
US20090246762A1 (en) * 2008-03-31 2009-10-01 Applera Corporation, Applied Biosystems Group Nucleic acid terminators incorporating a cationic moiety and methods for their use
CN102459469A (en) * 2009-04-17 2012-05-16 利康股份有限公司 Fluorescent imaging with substituted cyanine dyes
WO2012054749A1 (en) 2010-10-20 2012-04-26 Li-Cor, Inc. Cyanine dyes and their conjugates
WO2013041117A1 (en) 2011-09-23 2013-03-28 Illumina Cambrigde Ltd. Dyes for labelling molecular ligands
US8809551B1 (en) 2013-03-08 2014-08-19 Illumina Cambridge Limited Polymethine compounds and their use as fluorescent labels
GB201408077D0 (en) 2014-05-07 2014-06-18 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
GB201508858D0 (en) 2015-05-22 2015-07-01 Illumina Cambridge Ltd Polymethine compounds with long stokes shifts and their use as fluorescent labels
GB201516987D0 (en) 2015-09-25 2015-11-11 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340806C (en) 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US5242796A (en) 1986-07-02 1993-09-07 E. I. Du Pont De Nemours And Company Method, system and reagents for DNA sequencing
US5795762A (en) * 1986-08-22 1998-08-18 Roche Molecular Systems, Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5187085A (en) 1990-09-28 1993-02-16 Applied Biosystems, Inc. Nucleic acid sequence analysis with nucleoside-5'-o-(1-thiotriphosphates)
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5986086A (en) * 1997-06-20 1999-11-16 Amersham Pharmacia Biotech Inc. Non-sulfonated cyanine dyes for labeling nucleosides and nucleotides
JP4306960B2 (en) * 1998-02-04 2009-08-05 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション Dideoxy dye terminator

Also Published As

Publication number Publication date
DE69926057T2 (en) 2006-04-20
US6949635B1 (en) 2005-09-27
WO1999040223A1 (en) 1999-08-12
EP1060264B1 (en) 2005-07-06
DE69926057D1 (en) 2005-08-11
AU2571799A (en) 1999-08-23
EP1060264A4 (en) 2001-07-04
EP1060264A1 (en) 2000-12-20
ATE299190T1 (en) 2005-07-15
CA2319777C (en) 2008-01-08
JP2002505853A (en) 2002-02-26
JP4306960B2 (en) 2009-08-05

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MKLA Lapsed

Effective date: 20170202