CA2332628A1 - Seed-preferred promoters - Google Patents

Seed-preferred promoters Download PDF

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Publication number
CA2332628A1
CA2332628A1 CA002332628A CA2332628A CA2332628A1 CA 2332628 A1 CA2332628 A1 CA 2332628A1 CA 002332628 A CA002332628 A CA 002332628A CA 2332628 A CA2332628 A CA 2332628A CA 2332628 A1 CA2332628 A1 CA 2332628A1
Authority
CA
Canada
Prior art keywords
seed
preferred promoters
plant
heterologous nucleotide
nucleotide sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002332628A
Other languages
French (fr)
Other versions
CA2332628C (en
Inventor
Susan J. Martino-Catt
Kathryn K. Lappegard
Xun Wang
Benjamin A. Bowen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Hi Bred International Inc
Original Assignee
Pioneer Hi-Bred International, Inc.
Susan J. Martino-Catt
Kathryn K. Lappegard
Xun Wang
Benjamin A. Bowen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi-Bred International, Inc., Susan J. Martino-Catt, Kathryn K. Lappegard, Xun Wang, Benjamin A. Bowen filed Critical Pioneer Hi-Bred International, Inc.
Publication of CA2332628A1 publication Critical patent/CA2332628A1/en
Application granted granted Critical
Publication of CA2332628C publication Critical patent/CA2332628C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)

Abstract

The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant.
Compositions are novel nucleotide sequences for seed-preferred promoters isolated from genes for Cim1 (cytokinin-induced message);
cZ19B1 (maize 19kDa zein); or milps (myoinositol-1-phosphate synthase). A
method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the seed-preferred promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.
CA002332628A 1998-08-20 1999-08-17 Seed-preferred promoters Expired - Fee Related CA2332628C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9723398P 1998-08-20 1998-08-20
US60/097,233 1998-08-20
PCT/US1999/018628 WO2000011177A1 (en) 1998-08-20 1999-08-17 Seed-preferred promoters

Publications (2)

Publication Number Publication Date
CA2332628A1 true CA2332628A1 (en) 2000-03-02
CA2332628C CA2332628C (en) 2005-04-12

Family

ID=22262323

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002332628A Expired - Fee Related CA2332628C (en) 1998-08-20 1999-08-17 Seed-preferred promoters

Country Status (9)

Country Link
US (1) US6225529B1 (en)
EP (1) EP1104469B1 (en)
AR (1) AR022067A1 (en)
AT (1) ATE309362T1 (en)
AU (1) AU5489099A (en)
CA (1) CA2332628C (en)
DE (1) DE69928264T2 (en)
ES (1) ES2252964T3 (en)
WO (1) WO2000011177A1 (en)

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