CA2365418A1 - Phosphatases with improved phytase activity - Google Patents
Phosphatases with improved phytase activity Download PDFInfo
- Publication number
- CA2365418A1 CA2365418A1 CA002365418A CA2365418A CA2365418A1 CA 2365418 A1 CA2365418 A1 CA 2365418A1 CA 002365418 A CA002365418 A CA 002365418A CA 2365418 A CA2365418 A CA 2365418A CA 2365418 A1 CA2365418 A1 CA 2365418A1
- Authority
- CA
- Canada
- Prior art keywords
- phytase activity
- phosphatase
- phosphatases
- improved
- improved phytase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
Abstract
The present invention provides phosphatases with improved phytase activity.
The invention provides proteolytic fragments of phosphatase having improved phytase activity. A recombinant gene encoding a phosphatase fragment having improved phytase activity is also provided. The invention also includes a method of increasing the phytase activity of phosphatase by treating the phosphatase with a protease. In addition, the invention provides a phosphatase, AppA2, having improved properties.
The invention provides proteolytic fragments of phosphatase having improved phytase activity. A recombinant gene encoding a phosphatase fragment having improved phytase activity is also provided. The invention also includes a method of increasing the phytase activity of phosphatase by treating the phosphatase with a protease. In addition, the invention provides a phosphatase, AppA2, having improved properties.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12703299P | 1999-03-31 | 1999-03-31 | |
US60/127,032 | 1999-03-31 | ||
PCT/US2000/008590 WO2000058481A2 (en) | 1999-03-31 | 2000-03-31 | Phosphatases with improved phytase activity |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2365418A1 true CA2365418A1 (en) | 2000-10-05 |
CA2365418C CA2365418C (en) | 2009-05-26 |
Family
ID=22427982
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002365418A Expired - Lifetime CA2365418C (en) | 1999-03-31 | 2000-03-31 | Phosphatases with improved phytase activity |
Country Status (12)
Country | Link |
---|---|
US (2) | US6511699B1 (en) |
EP (1) | EP1165806B1 (en) |
CN (1) | CN100347303C (en) |
AT (1) | ATE301198T1 (en) |
AU (1) | AU4056700A (en) |
BR (1) | BR0009516B1 (en) |
CA (1) | CA2365418C (en) |
DE (1) | DE60021715T2 (en) |
DK (1) | DK1165806T3 (en) |
ES (1) | ES2248067T3 (en) |
MX (1) | MXPA01009809A (en) |
WO (1) | WO2000058481A2 (en) |
Families Citing this family (28)
Publication number | Priority date | Publication date | Assignee | Title |
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US7078035B2 (en) | 1997-08-13 | 2006-07-18 | Diversa Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
US6720014B1 (en) | 1997-08-13 | 2004-04-13 | Diversa Corporation | Phytase-containing foodstuffs and methods of making and using them |
US6183740B1 (en) | 1997-08-13 | 2001-02-06 | Diversa Corporation | Recombinant bacterial phytases and uses thereof |
US7432097B2 (en) | 1997-08-13 | 2008-10-07 | Verenium Corporation | Phytases, nucleic acids encoding them and methods of making and using them |
US6451572B1 (en) | 1998-06-25 | 2002-09-17 | Cornell Research Foundation, Inc. | Overexpression of phytase genes in yeast systems |
US6841370B1 (en) | 1999-11-18 | 2005-01-11 | Cornell Research Foundation, Inc. | Site-directed mutagenesis of Escherichia coli phytase |
WO2003018766A2 (en) | 2001-08-27 | 2003-03-06 | Syngenta Participations Ag | Self-processing plants and plant parts |
EP1450627B1 (en) * | 2001-10-31 | 2012-09-05 | Phytex, Llc | Use of phytase containing animal food |
MY139056A (en) | 2001-12-28 | 2009-08-28 | Ab Enzymes Gmbh | Microbially-expressed thermotolerant phytase for animal feed |
TW200305430A (en) * | 2001-12-28 | 2003-11-01 | Syngenta Participations Ag | Thermotolerant phytase for animal feed |
WO2004024885A2 (en) | 2002-09-13 | 2004-03-25 | Cornell Research Foundation, Inc. | Using mutations to improve aspergillus phytases |
US7442398B2 (en) * | 2003-03-25 | 2008-10-28 | Republic Of National Fisheries Research And Development Institute | Phytase produced from Citrobacter braakii |
PL2160950T3 (en) * | 2004-09-27 | 2015-10-30 | Novozymes As | Enzyme granules |
US7658922B2 (en) | 2005-06-24 | 2010-02-09 | Ab Enzymes Gmbh | Monoclonal antibodies, hybridoma cell lines, methods and kits for detecting phytase |
US7919297B2 (en) | 2006-02-21 | 2011-04-05 | Cornell Research Foundation, Inc. | Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase |
US8540984B2 (en) * | 2006-08-03 | 2013-09-24 | Cornell Research Foundation, Inc. | Phytases with improved thermal stability |
EP2617729B1 (en) * | 2006-09-21 | 2016-03-16 | BASF Enzymes LLC | Phytases, nucleic acids encoding them and methods for making and using them |
US7923232B2 (en) * | 2007-03-26 | 2011-04-12 | Novozymes A/S | Hafnia phytase |
US8192734B2 (en) | 2007-07-09 | 2012-06-05 | Cornell University | Compositions and methods for bone strengthening |
EP2342323B1 (en) * | 2008-09-26 | 2013-06-05 | Novozymes A/S | Hafnia phytase variants |
BRPI1011160A8 (en) | 2009-05-21 | 2018-01-02 | Verenium Corp | PHYTASES, PROTEIN PREPARATION INCLUDING THEM, AND THEIR USES |
EP3594351B1 (en) | 2013-03-08 | 2021-09-29 | Keck Graduate Institute of Applied Life Sciences | Yeast promoters from pichia pastoris |
ES2716619T3 (en) | 2013-03-08 | 2019-06-13 | Biogrammatics Inc | Yeast promoters for protein expression |
CN104703170B (en) | 2013-12-05 | 2017-04-12 | 华为终端有限公司 | Methods and equipment for downloading file of operator |
CN105637498B (en) | 2014-05-23 | 2019-05-28 | 华为技术有限公司 | Management method, eUICC, SM platform and the system of eUICC |
US9605245B1 (en) | 2016-06-30 | 2017-03-28 | Fornia BioSoultions, Inc. | Phytases and uses thereof |
US10351832B2 (en) | 2016-06-30 | 2019-07-16 | Fornia Biosolutions, Inc. | Phytases and uses thereof |
US9528096B1 (en) * | 2016-06-30 | 2016-12-27 | Fornia Biosolutions, Inc. | Phytases and uses thereof |
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-
2000
- 2000-03-31 AT AT00919962T patent/ATE301198T1/en not_active IP Right Cessation
- 2000-03-31 ES ES00919962T patent/ES2248067T3/en not_active Expired - Lifetime
- 2000-03-31 CA CA002365418A patent/CA2365418C/en not_active Expired - Lifetime
- 2000-03-31 MX MXPA01009809A patent/MXPA01009809A/en active IP Right Grant
- 2000-03-31 CN CNB008081417A patent/CN100347303C/en not_active Expired - Lifetime
- 2000-03-31 EP EP00919962A patent/EP1165806B1/en not_active Expired - Lifetime
- 2000-03-31 DE DE60021715T patent/DE60021715T2/en not_active Expired - Lifetime
- 2000-03-31 US US09/540,149 patent/US6511699B1/en not_active Expired - Lifetime
- 2000-03-31 AU AU40567/00A patent/AU4056700A/en not_active Abandoned
- 2000-03-31 DK DK00919962T patent/DK1165806T3/en active
- 2000-03-31 BR BRPI0009516-8B1A patent/BR0009516B1/en active IP Right Grant
- 2000-03-31 WO PCT/US2000/008590 patent/WO2000058481A2/en active IP Right Grant
-
2002
- 2002-10-07 US US10/266,041 patent/US6974690B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
WO2000058481A3 (en) | 2001-01-04 |
DE60021715T2 (en) | 2006-06-01 |
MXPA01009809A (en) | 2002-07-30 |
WO2000058481A9 (en) | 2002-08-29 |
EP1165806B1 (en) | 2005-08-03 |
AU4056700A (en) | 2000-10-16 |
ES2248067T3 (en) | 2006-03-16 |
WO2000058481A2 (en) | 2000-10-05 |
US6511699B1 (en) | 2003-01-28 |
US20030072844A1 (en) | 2003-04-17 |
ATE301198T1 (en) | 2005-08-15 |
CN1413253A (en) | 2003-04-23 |
DK1165806T3 (en) | 2005-12-05 |
US6974690B2 (en) | 2005-12-13 |
EP1165806A2 (en) | 2002-01-02 |
CA2365418C (en) | 2009-05-26 |
CN100347303C (en) | 2007-11-07 |
BR0009516B1 (en) | 2013-09-10 |
DE60021715D1 (en) | 2005-09-08 |
BR0009516A (en) | 2001-12-26 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKEX | Expiry |
Effective date: 20200331 |