CA2365418A1 - Phosphatases with improved phytase activity - Google Patents

Phosphatases with improved phytase activity Download PDF

Info

Publication number
CA2365418A1
CA2365418A1 CA002365418A CA2365418A CA2365418A1 CA 2365418 A1 CA2365418 A1 CA 2365418A1 CA 002365418 A CA002365418 A CA 002365418A CA 2365418 A CA2365418 A CA 2365418A CA 2365418 A1 CA2365418 A1 CA 2365418A1
Authority
CA
Canada
Prior art keywords
phytase activity
phosphatase
phosphatases
improved
improved phytase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002365418A
Other languages
French (fr)
Other versions
CA2365418C (en
Inventor
Xingen Lei
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cornell Research Foundation Inc
Original Assignee
Cornell Research Foundation, Inc.
Xingen Lei
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cornell Research Foundation, Inc., Xingen Lei filed Critical Cornell Research Foundation, Inc.
Publication of CA2365418A1 publication Critical patent/CA2365418A1/en
Application granted granted Critical
Publication of CA2365418C publication Critical patent/CA2365418C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines

Abstract

The present invention provides phosphatases with improved phytase activity.
The invention provides proteolytic fragments of phosphatase having improved phytase activity. A recombinant gene encoding a phosphatase fragment having improved phytase activity is also provided. The invention also includes a method of increasing the phytase activity of phosphatase by treating the phosphatase with a protease. In addition, the invention provides a phosphatase, AppA2, having improved properties.
CA002365418A 1999-03-31 2000-03-31 Phosphatases with improved phytase activity Expired - Lifetime CA2365418C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12703299P 1999-03-31 1999-03-31
US60/127,032 1999-03-31
PCT/US2000/008590 WO2000058481A2 (en) 1999-03-31 2000-03-31 Phosphatases with improved phytase activity

Publications (2)

Publication Number Publication Date
CA2365418A1 true CA2365418A1 (en) 2000-10-05
CA2365418C CA2365418C (en) 2009-05-26

Family

ID=22427982

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002365418A Expired - Lifetime CA2365418C (en) 1999-03-31 2000-03-31 Phosphatases with improved phytase activity

Country Status (12)

Country Link
US (2) US6511699B1 (en)
EP (1) EP1165806B1 (en)
CN (1) CN100347303C (en)
AT (1) ATE301198T1 (en)
AU (1) AU4056700A (en)
BR (1) BR0009516B1 (en)
CA (1) CA2365418C (en)
DE (1) DE60021715T2 (en)
DK (1) DK1165806T3 (en)
ES (1) ES2248067T3 (en)
MX (1) MXPA01009809A (en)
WO (1) WO2000058481A2 (en)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6183740B1 (en) 1997-08-13 2001-02-06 Diversa Corporation Recombinant bacterial phytases and uses thereof
US6720014B1 (en) 1997-08-13 2004-04-13 Diversa Corporation Phytase-containing foodstuffs and methods of making and using them
US7432097B2 (en) 1997-08-13 2008-10-07 Verenium Corporation Phytases, nucleic acids encoding them and methods of making and using them
US7078035B2 (en) 1997-08-13 2006-07-18 Diversa Corporation Phytases, nucleic acids encoding them and methods for making and using them
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
US6841370B1 (en) 1999-11-18 2005-01-11 Cornell Research Foundation, Inc. Site-directed mutagenesis of Escherichia coli phytase
AR036370A1 (en) 2001-08-27 2004-09-01 Syngenta Participations Ag PLANTS WITH SELF-PROCESSING AND PARTS OF THE SAME
AU2002356880A1 (en) * 2001-10-31 2003-05-12 Phytex, Llc Phytase-containing animal food and method
TW200305430A (en) 2001-12-28 2003-11-01 Syngenta Participations Ag Thermotolerant phytase for animal feed
TWI262083B (en) 2001-12-28 2006-09-21 Syngenta Participations Ag Microbially-expressed thermotolerant phytase for animal feed
US7309505B2 (en) * 2002-09-13 2007-12-18 Cornell Research Foundation, Inc. Using mutations to improve Aspergillus phytases
US7442398B2 (en) * 2003-03-25 2008-10-28 Republic Of National Fisheries Research And Development Institute Phytase produced from Citrobacter braakii
PL2160950T3 (en) * 2004-09-27 2015-10-30 Novozymes As Enzyme granules
US7658922B2 (en) 2005-06-24 2010-02-09 Ab Enzymes Gmbh Monoclonal antibodies, hybridoma cell lines, methods and kits for detecting phytase
US7919297B2 (en) * 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
WO2008017066A2 (en) 2006-08-03 2008-02-07 Cornell Research Foundation, Inc. Phytases with improved thermal stability
EP2617819B1 (en) * 2006-09-21 2016-04-27 BASF Enzymes LLC Phytases, nucleic acids encoding them and methods for making and using them
DK2129781T3 (en) * 2007-03-26 2014-03-31 Novozymes As Hafnia phytase
US8192734B2 (en) 2007-07-09 2012-06-05 Cornell University Compositions and methods for bone strengthening
MX2011003132A (en) * 2008-09-26 2011-04-21 Novozymes As Hafnia phytase variants.
MX355638B (en) 2009-05-21 2018-03-26 Verenium Corp Phytases, nucleic acids encoding them and methods for making and using them.
CN105358694B (en) 2013-03-08 2019-06-18 凯克应用生命科学研究生院 Yeast promoter from pichia pastoris yeast
PT2964775T (en) 2013-03-08 2019-05-06 Biogrammatics Inc Yeast promoters for protein expression
CN104703170B (en) 2013-12-05 2017-04-12 华为终端有限公司 Methods and equipment for downloading file of operator
KR102126010B1 (en) 2014-05-23 2020-06-23 후아웨이 테크놀러지 컴퍼니 리미티드 Euicc management method, euicc, sm platform and system
US10351832B2 (en) 2016-06-30 2019-07-16 Fornia Biosolutions, Inc. Phytases and uses thereof
US9528096B1 (en) * 2016-06-30 2016-12-27 Fornia Biosolutions, Inc. Phytases and uses thereof
US9605245B1 (en) 2016-06-30 2017-03-28 Fornia BioSoultions, Inc. Phytases and uses thereof

Family Cites Families (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US554399A (en) * 1896-02-11 Charles e
US3431195A (en) * 1967-04-17 1969-03-04 Universal Oil Prod Co Purifying make hydrogen in a catalytic reforming process
US3430418A (en) * 1967-08-09 1969-03-04 Union Carbide Corp Selective adsorption process
CA940070A (en) 1968-12-23 1974-01-15 Jim S. Berry Stabilized aqueous enzyme composition
US3564816A (en) * 1968-12-30 1971-02-23 Union Carbide Corp Selective adsorption process
US3860484A (en) 1972-09-28 1975-01-14 Xerox Corp Enzyme stabilization
DE2426988C2 (en) 1974-06-04 1985-02-14 Boehringer Mannheim Gmbh, 6800 Mannheim Method for carrier binding of biologically active proteins
US3966971A (en) 1975-01-02 1976-06-29 Grain Processing Corporation Separation of protein from vegetable sources
US3986849A (en) * 1975-11-07 1976-10-19 Union Carbide Corporation Selective adsorption process
DE2931999A1 (en) 1979-08-03 1981-02-26 Schering Ag PRODUCTION AND USE OF RECOMBINED PLASMIDES WITH GENES FOR ALKALINE PHOSPHATASES
DE3126759A1 (en) 1981-07-07 1983-01-27 Boehringer Mannheim Gmbh, 6800 Mannheim SOLUBLE LIVER URICASE, METHOD FOR THE PRODUCTION AND USE THEREOF
JPS5931799A (en) 1982-08-16 1984-02-20 Science & Tech Agency Recombinant plasmid and preparation of transformed yeast and hepatitis virus b surface antigen using the same
US4470968A (en) 1983-01-13 1984-09-11 Miles Laboratories, Inc. Pasteurized therapeutically active blood coagulation factor concentrates
SE450325B (en) 1983-02-23 1987-06-22 Tricum Ab FOOD FIBER PRODUCT BASED ON FOOD PARTS FROM FROZEN BY CERALIER
US4547205A (en) * 1984-10-11 1985-10-15 Uop Inc. Dehydrocyclodimerization process
SE465951B (en) 1984-10-23 1991-11-25 Perstorp Ab ISOMER OF INOSITOL TRIPHOSPHATE PHARMACEUTICAL STATEMENTS FOR SALT FOR USE AS THERAPEUTIC OR PROPHYLACTIC AGENTS AND COMPOSITIONS THEREOF
DE3515586A1 (en) 1985-04-30 1986-11-06 Boehringer Mannheim Gmbh, 6800 Mannheim STABILIZED SARCOSINOXIDASE PREPARATION
US5024941A (en) 1985-12-18 1991-06-18 Biotechnica International, Inc. Expression and secretion vector for yeast containing a glucoamylase signal sequence
JPH0655146B2 (en) 1985-12-27 1994-07-27 財団法人化学及血清療法研究所 Shuttle vector
US5780292A (en) 1987-04-29 1998-07-14 Alko Group Ltd. Production of phytate degrading enzymes in trichoderma
US4915029A (en) * 1987-03-05 1990-04-10 Halliburton Company Shaped charge carrier assembly method
NL8702735A (en) 1987-11-17 1989-06-16 Dorr Oliver Inc METHOD FOR SOAKING CEREALS WITH A NEW ENZYME PREPARATION.
GB8826429D0 (en) 1988-11-11 1988-12-14 Univ Leeds Ind Service Ltd Enzyme stabilisation systems
US5316770A (en) 1989-02-16 1994-05-31 University Of Georgia Research Foundation, Inc. Vitamin D derivative feed compositions and methods of use
US5366736A (en) 1989-02-16 1994-11-22 University Of Georgia Research Foundation, Inc. Vitamin D derivative feed compositions and methods of use
CZ289014B6 (en) 1989-09-27 2001-10-17 Dsm N. V. Purified and isolated DNA sequence encoding fungal phytase, construct for expression, vectors and transformed host cells as well as phytase preparation process
UA27702C2 (en) 1989-09-27 2000-10-16 Гіст-Брокейдс Н.В. Fragment of genomic dna coding phytase aspergillus niger, fragment of cdna coding phytase aspergillus niger, recombinant plasmid dna for expression of phytase in aspergillus (variants), strain aspergillus producent of aspergillus (variants), process for praparation
US5012037A (en) * 1990-01-10 1991-04-30 Uop Integrated thermal swing-pressure swing adsorption process for hydrogen and hydrocarbon recovery
PT97110B (en) 1990-03-23 1998-11-30 Gist Brocades Nv PROCESS FOR CATALYSING ACCELERABLE REACTIONS BY ENZYMES, BY ADDING TO THE REACTIONAL MEDIUM OF TRANSGENIC PLANTS SEEDS AND FOR OBTAINING THE REFERED SEEDS
KR100225087B1 (en) 1990-03-23 1999-10-15 한스 발터라벤 The expression of phytase in plants
GB9006642D0 (en) 1990-03-24 1990-05-23 Gibson Timothy D Enzyme stabilisation
DE4011084A1 (en) 1990-04-05 1991-10-10 Boehringer Mannheim Gmbh SACCHARID-MODIFIED, WATER-SOLUBLE PROTEINS
US5200399A (en) 1990-09-14 1993-04-06 Boyce Thompson Institute For Plant Research, Inc. Method of protecting biological materials from destructive reactions in the dry state
EP0513332A4 (en) 1990-11-14 1993-03-17 Cargill, Incorporated Conjugates of poly(vinylsaccharide) with proteins for the stabilization of proteins
US5268273A (en) 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
JP2981302B2 (en) * 1991-05-13 1999-11-22 東洋エンジニアリング株式会社 Gas separation method
DE4119281A1 (en) 1991-06-12 1992-12-17 Basf Ag METHOD FOR PRODUCING ENZYME PREPARATIONS
US5178751A (en) * 1991-11-27 1993-01-12 Uop Two-stage process for purifying a hydrogen gas and recovering liquifiable hydrocarbons from hydrocarbonaceous effluent streams
CA2106774C (en) 1992-01-24 2005-01-04 Reinder Sietze Hamstra Method for the preparation of feed pellets
IL104704A0 (en) 1992-02-13 1993-06-10 Gist Brocades Nv Stabilized aqueous liquid formulation of phytase
WO1994003072A1 (en) 1992-07-31 1994-02-17 Panlabs Incorporated Recombinant cells, dna constructs, vectors and methods for expressing phytate degrading enzymes in desired ratios
ES2202305T3 (en) 1993-04-05 2004-04-01 Aveve N.V. HYDROLYSIS OF FITINA AND COMPOSITION OF ENZYMES THAT HAVE HYDROLYZING ACTIVITY OF FITATE.
JP2696057B2 (en) 1993-05-11 1998-01-14 ニチモウ株式会社 Method for producing product from cereals
FR2715802B1 (en) 1994-02-04 1996-03-15 Rhone Poulenc Nutrition Animal Use of enzymes in animal feed to reduce nitrogen emissions.
US5955448A (en) 1994-08-19 1999-09-21 Quadrant Holdings Cambridge Limited Method for stabilization of biological substances during drying and subsequent storage and compositions thereof
EP0756457B2 (en) 1994-04-22 2008-10-08 Novozymes A/S A method for improving the solubility of vegetable proteins
DK0684313T3 (en) 1994-04-25 2006-11-06 Dsm Ip Assets Bv Phytase activity polypeptides
US5830732A (en) 1994-07-05 1998-11-03 Mitsui Toatsu Chemicals, Inc. Phytase
GB9416841D0 (en) 1994-08-19 1994-10-12 Finnfeeds Int Ltd An enzyme feed additive and animal feed including it
FR2729971B1 (en) 1995-01-31 1997-06-06 Roquette Freres NUTRITIONAL COMPOSITION RESULTING FROM CORN QUENCHING AND PROCESS FOR OBTAINING SAME
US5935624A (en) 1995-02-06 1999-08-10 Wisconsin Alumni Research Foundation Low phosphorus animal feed containing 1α-hydroxylated vitamin D compounds and method of preparing
US5556771A (en) 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
SK40697A3 (en) 1995-07-28 1997-10-08 Gist Brocades Bv Salt-stabilized enzyme preparations
CN1201372A (en) 1995-11-02 1998-12-09 诺沃挪第克公司 Feed enzyme products
DK172530B1 (en) 1995-11-10 1998-11-23 Leo Pharm Prod Ltd Additive product for drinking water and animal feed and method of addition
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
AU2077197A (en) 1996-03-18 1997-10-10 Novo Nordisk Biotech, Inc. Polypeptides having phytase activity and nucleic acids encoding same
EA199800891A1 (en) 1996-04-05 1999-04-29 Киова Хакко Когио Ко., Лтд NEW PHITASIS AND GENES ENCODING THE INDICATED FITAZ
EP0902623A1 (en) 1996-04-23 1999-03-24 Novo Nordisk A/S Animal feed additives
AU3214597A (en) 1996-05-29 1998-01-05 Universal Preservation Technologies, Inc. Long-term shelf preservation by vitrification
US5985605A (en) 1996-06-14 1999-11-16 Her Majesty The Queen In Right Of Canada, As Represented By The Dept. Of Agriculture & Agri-Food Canada DNA sequences encoding phytases of ruminal microorganisms
FR2751333B1 (en) 1996-07-18 1998-09-25 Roquette Freres IMPROVED NUTRITIONAL COMPOSITION RESULTING FROM CORN QUENCHING AND PROCESS FOR OBTAINING SAME
FR2751987B1 (en) 1996-08-01 1998-12-31 Biocem PLANT PHYTASES AND BIOTECHNOLOGICAL APPLICATIONS
GB2316082A (en) 1996-08-13 1998-02-18 Finnfeeds Int Ltd Phytase
SE507355C2 (en) 1996-09-18 1998-05-18 Semper Ab Procedure for reducing the content of grains in grains
PL332375A1 (en) 1996-09-25 1999-09-13 Kyowa Hakko Kogyo Kk Novel phytases and method of obtaining them
GB2319030A (en) 1996-11-05 1998-05-13 Finnfeeds Int Ltd Phytase extracted from soybean
US6039942A (en) 1996-12-20 2000-03-21 Novo Nordick A/S Phytase polypeptides
WO1998030681A1 (en) 1997-01-09 1998-07-16 Novo Nordisk A/S Phytase combinations
KR100222638B1 (en) 1997-01-20 1999-10-01 배희동 Process for producing enzymes using the seeds
CA2231948C (en) 1997-03-25 2010-05-18 F. Hoffmann-La Roche Ag Modified phytases
KR100206453B1 (en) 1997-03-27 1999-07-01 박원훈 A novel strain e coli jm83/pkp2 transformed with a novel plasmid and phytase produced from it
CN1151256C (en) 1997-05-28 2004-05-26 基本应用私人有限公司 Enhancement of industrial enzymes
NZ330940A (en) 1997-07-24 2000-02-28 F Production of consensus phytases from fungal origin using computer programmes
US6183740B1 (en) 1997-08-13 2001-02-06 Diversa Corporation Recombinant bacterial phytases and uses thereof
US6720014B1 (en) * 1997-08-13 2004-04-13 Diversa Corporation Phytase-containing foodstuffs and methods of making and using them
US5876997A (en) 1997-08-13 1999-03-02 Diversa Corporation Phytase
US6022555A (en) 1997-09-05 2000-02-08 Wisconsin Alumni Research Foundation Animal feed containing carboxylic acids
DK0911416T3 (en) 1997-10-01 2006-08-14 Dsm Ip Assets Bv Process for producing protein
DE19743683A1 (en) 1997-10-02 1999-04-08 Basf Ag Procedure for changing the substrate specificity of enzymes
DE69720478T2 (en) 1997-12-23 2003-12-18 Cargill Bv Animal feed containing protein and method of manufacture
US20010018197A1 (en) 1997-12-23 2001-08-30 Protein Technologies International, Inc. Method for producing ultrapure vegetable protein materials
CN1192103C (en) 1998-01-27 2005-03-09 三井化学株式会社 Method for producing phytase
US6284502B1 (en) 1998-08-21 2001-09-04 University Of Saskatchewan Process for converting phytate into inorganic phosphate
FR2804691B1 (en) 2000-02-04 2003-11-07 Roquette Freres NITROGEN COMPOSITION RESULTING FROM HYDROLYSIS OF CORN GLUTEN AND METHOD FOR PRODUCING THE SAME

Also Published As

Publication number Publication date
EP1165806A2 (en) 2002-01-02
EP1165806B1 (en) 2005-08-03
CN1413253A (en) 2003-04-23
AU4056700A (en) 2000-10-16
BR0009516A (en) 2001-12-26
MXPA01009809A (en) 2002-07-30
WO2000058481A3 (en) 2001-01-04
WO2000058481A9 (en) 2002-08-29
WO2000058481A2 (en) 2000-10-05
ES2248067T3 (en) 2006-03-16
DE60021715D1 (en) 2005-09-08
BR0009516B1 (en) 2013-09-10
CN100347303C (en) 2007-11-07
US6511699B1 (en) 2003-01-28
ATE301198T1 (en) 2005-08-15
CA2365418C (en) 2009-05-26
DE60021715T2 (en) 2006-06-01
DK1165806T3 (en) 2005-12-05
US20030072844A1 (en) 2003-04-17
US6974690B2 (en) 2005-12-13

Similar Documents

Publication Publication Date Title
CA2365418A1 (en) Phosphatases with improved phytase activity
EP2333098A3 (en) Enzymes having alpha amylase activity and methods of use thereof
WO2002000860A3 (en) Novel proteases
WO2002009641A3 (en) Promyostatin peptides and methods of using same
WO1998056927A3 (en) Nucleic acids encoding polypeptide having protease activity
EP0783527A4 (en) Cathepsin o2 protease
AU3802595A (en) Tripeptidyl aminopeptidase
LT99131A (en) Compounds and compositions for treating diseases associated with serine protease, particularly tryptase, acitivity
WO2001016324A3 (en) Methods and compositions of a novel serine protease inhibitor
DK0672150T3 (en) Modified Kluyveromyces yeast, manufacture and use
EP1757684A3 (en) A 2,6-beta-D-fructan hydrolase enzyme and processes for using the enzyme
EP0897011A3 (en) Novel arginine deiminase
EP0967286A3 (en) Leucine aminopeptidase gene, recombinant DNA, and process for producing leucine aminopeptidase
EP0931835A3 (en) Method for cloning and producing the PmeI restriction endonuclease
WO2002018536A3 (en) Truncated bard1 protein, and its diagnostic and therapeutic uses
WO2000052147A3 (en) Bacterial prolyl peptidases and methods of use
AU6181100A (en) Gene encoding novel serine protease-like protein
WO2001040484A3 (en) A novel polypeptide, a human atp-dependent serine protease 64 and the polynucleotide encoding the polypeptide
WO2001083675A3 (en) A novel polypeptide, a atp-dependent human hydrolase serine 10.2 and the polynucleotide encoding the polypeptide
WO2001075042A3 (en) A novel polypeptide, atp-dependent human hydrolase serine 9 and the polynucleotide encoding the polypeptide
WO2001075004A3 (en) A novel polypeptide, ubiquitine 9 conjugation enzyme and the polynucleotide encoding the polypeptide
WO2001074998A3 (en) Novel polypeptide - a human protein hydrolytic enzyme regulatory protein 10 and polynucleotide encoding it
WO2001075009A3 (en) A novel polypeptide, arnt synthetase serine 27 and the polynucleotide encoding the polypeptide
AU2001277707A1 (en) Promoter, vector and recombinant microorganism having the same and process for producing protein
WO2001077285A3 (en) A novel polypeptide, protein 13 of the amidase families and the polynucleotide encoding the polypeptide

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20200331