CA2374667A1 - Synthetic ligation reassembly in directed evolution - Google Patents
Synthetic ligation reassembly in directed evolution Download PDFInfo
- Publication number
- CA2374667A1 CA2374667A1 CA002374667A CA2374667A CA2374667A1 CA 2374667 A1 CA2374667 A1 CA 2374667A1 CA 002374667 A CA002374667 A CA 002374667A CA 2374667 A CA2374667 A CA 2374667A CA 2374667 A1 CA2374667 A1 CA 2374667A1
- Authority
- CA
- Canada
- Prior art keywords
- directed evolution
- ligation reassembly
- synthetic ligation
- directed
- polynucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000040430 polynucleotide Human genes 0.000 abstract 3
- 108091033319 polynucleotide Proteins 0.000 abstract 3
- 239000002157 polynucleotide Substances 0.000 abstract 3
- 238000010367 cloning Methods 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
- A61P33/06—Antimalarials
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
- C07K14/445—Plasmodium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y308/00—Hydrolases acting on halide bonds (3.8)
- C12Y308/01—Hydrolases acting on halide bonds (3.8) in C-halide substances (3.8.1)
- C12Y308/01002—(S)-2-Haloacid dehalogenase (3.8.1.2)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/53—DNA (RNA) vaccination
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
Abstract
The invention is directed to a process of preparing nucleic acid and poly nucleotide libraries by cloning non-randomly generated nucleotide fragments having ligatable ends into an expression vector. The expression of the polynucleotide provides an indirect means by which to screen the polynucleotide library.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/332,835 US6537776B1 (en) | 1999-06-14 | 1999-06-14 | Synthetic ligation reassembly in directed evolution |
US09/332,835 | 1999-06-14 | ||
PCT/US2000/016838 WO2000077262A1 (en) | 1999-06-14 | 2000-06-14 | Synthetic ligation reassembly in directed evolution |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2374667A1 true CA2374667A1 (en) | 2000-12-21 |
CA2374667C CA2374667C (en) | 2008-08-26 |
Family
ID=23300064
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002374667A Expired - Fee Related CA2374667C (en) | 1999-06-14 | 2000-06-14 | Synthetic ligation reassembly in directed evolution |
Country Status (9)
Country | Link |
---|---|
US (5) | US6537776B1 (en) |
EP (3) | EP2397549A3 (en) |
JP (1) | JP4699662B2 (en) |
AU (1) | AU5624600A (en) |
CA (1) | CA2374667C (en) |
HK (1) | HK1045858A1 (en) |
IL (2) | IL146937A0 (en) |
MX (1) | MXPA01013020A (en) |
WO (1) | WO2000077262A1 (en) |
Families Citing this family (174)
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2000
- 2000-02-04 EP EP11167668A patent/EP2397549A3/en not_active Ceased
- 2000-06-14 WO PCT/US2000/016838 patent/WO2000077262A1/en active Application Filing
- 2000-06-14 US US09/594,459 patent/US6605449B1/en not_active Ceased
- 2000-06-14 JP JP2001503702A patent/JP4699662B2/en not_active Expired - Fee Related
- 2000-06-14 MX MXPA01013020A patent/MXPA01013020A/en active IP Right Grant
- 2000-06-14 AU AU56246/00A patent/AU5624600A/en not_active Abandoned
- 2000-06-14 EP EP00941550A patent/EP1192280A4/en not_active Ceased
- 2000-06-14 EP EP20140195944 patent/EP2865754A1/en not_active Withdrawn
- 2000-06-14 CA CA002374667A patent/CA2374667C/en not_active Expired - Fee Related
- 2000-06-14 IL IL14693700A patent/IL146937A0/en unknown
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2001
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2002
- 2002-10-03 HK HK02107285.7A patent/HK1045858A1/en unknown
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2003
- 2003-04-24 US US10/422,523 patent/US20040002103A1/en not_active Abandoned
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2007
- 2007-05-09 US US11/798,032 patent/USRE45349E1/en not_active Expired - Lifetime
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2014
- 2014-12-23 US US14/581,571 patent/US20150119293A1/en not_active Abandoned
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WO2000077262A9 (en) | 2002-06-13 |
IL146937A (en) | 2010-11-30 |
JP4699662B2 (en) | 2011-06-15 |
EP2865754A1 (en) | 2015-04-29 |
EP1192280A4 (en) | 2006-04-05 |
HK1045858A1 (en) | 2002-12-13 |
IL146937A0 (en) | 2002-08-14 |
JP2004500027A (en) | 2004-01-08 |
EP2397549A3 (en) | 2013-02-20 |
US6537776B1 (en) | 2003-03-25 |
AU5624600A (en) | 2001-01-02 |
EP2397549A2 (en) | 2011-12-21 |
EP1192280A1 (en) | 2002-04-03 |
US20150119293A1 (en) | 2015-04-30 |
US20040002103A1 (en) | 2004-01-01 |
US6605449B1 (en) | 2003-08-12 |
MXPA01013020A (en) | 2002-07-22 |
WO2000077262A1 (en) | 2000-12-21 |
CA2374667C (en) | 2008-08-26 |
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