CA2381506A1 - Difference detection methods using matched multiple dyes - Google Patents

Difference detection methods using matched multiple dyes Download PDF

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Publication number
CA2381506A1
CA2381506A1 CA002381506A CA2381506A CA2381506A1 CA 2381506 A1 CA2381506 A1 CA 2381506A1 CA 002381506 A CA002381506 A CA 002381506A CA 2381506 A CA2381506 A CA 2381506A CA 2381506 A1 CA2381506 A1 CA 2381506A1
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CA
Canada
Prior art keywords
proteins
dyes
sample
different
samples
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002381506A
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French (fr)
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CA2381506C (en
Inventor
Jonathan Minden
Alan Waggoner
Susan Janet Fowler
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GE Healthcare UK Ltd
Carnegie Mellon University
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Individual
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Publication of CA2381506A1 publication Critical patent/CA2381506A1/en
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Publication of CA2381506C publication Critical patent/CA2381506C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6827—Total protein determination, e.g. albumin in urine
    • G01N33/6839—Total protein determination, e.g. albumin in urine involving dyes, e.g. Coomassie blue, bromcresol green
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2550/00—Electrophoretic profiling, e.g. for proteome analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/17—Nitrogen containing
    • Y10T436/173845—Amine and quaternary ammonium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/20—Oxygen containing
    • Y10T436/203332—Hydroxyl containing

Abstract

A process and a kit are provided for detecting differences in two or more samples of protein, including proteins bearing post-translational modifications and peptides.
Proteins are prepared, for example, from each of a different group of cell samples or body fluid samples to be compared. Each protein extract is labelled with a different one of a luminescent dye from a matched set of dyes. The matched dyes have generally the same ionic and pH
characteristics but emit light at different wavelengths to exhibit a different color upon luminescence detection. The labelled protein extracts are mixed together and separated together by electrophoresis or a chromatographic method. The separation is observed to detect proteins unique to one sample or present in a greater ratio in one sample than in the other. Those unique or excess proteins will fluoresce the color of one of the dyes used.
Proteins common to each sample migrate together and fluoresce the same.
CA002381506A 1999-08-09 2000-08-09 Difference detection methods using matched multiple dyes Expired - Lifetime CA2381506C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/370,743 US6426190B1 (en) 1995-04-20 1999-08-09 Difference detection methods using matched multiple dyes
US09/370,743 1999-08-09
PCT/US2000/021766 WO2001011373A2 (en) 1999-08-09 2000-08-09 Difference detection methods using matched multiple dyes

Publications (2)

Publication Number Publication Date
CA2381506A1 true CA2381506A1 (en) 2001-02-15
CA2381506C CA2381506C (en) 2004-07-27

Family

ID=23460976

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002381506A Expired - Lifetime CA2381506C (en) 1999-08-09 2000-08-09 Difference detection methods using matched multiple dyes

Country Status (9)

Country Link
US (3) US6426190B1 (en)
EP (1) EP1200833B1 (en)
JP (1) JP4589587B2 (en)
AT (1) ATE283486T1 (en)
AU (2) AU778065B2 (en)
CA (1) CA2381506C (en)
DE (1) DE60016233T2 (en)
ES (1) ES2233421T3 (en)
WO (1) WO2001011373A2 (en)

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JP2003048879A (en) 2001-05-31 2003-02-21 Nisshinbo Ind Inc Fluorescent group-containing carbodiimide compound and method for manufacturing the compound
US6972326B2 (en) * 2001-12-03 2005-12-06 Molecular Probes, Inc. Labeling of immobilized proteins using dipyrrometheneboron difluoride dyes
EP1319954A1 (en) * 2001-12-12 2003-06-18 Centre National de Genotypage Methods for protein analysis using protein capture arrays
AU2003280470A1 (en) * 2002-07-01 2004-01-19 Guava Technologies, Inc. Fluorescent dyes, energy transfer couples and methods
US7582260B2 (en) * 2002-07-18 2009-09-01 Montana State University Zwitterionic dyes for labeling in proteomic and other biological analyses
AU2003234038B2 (en) * 2003-05-28 2008-02-21 Ge Healthcare Uk Limited Differential analysis of cell surface proteins on closed membrane structures by labelling with dyes in the presence of an internal standard
US7625758B2 (en) * 2005-01-26 2009-12-01 Berkelman Thomas R Coumarin-based cyanine dyes for non-specific protein binding
WO2007021501A1 (en) * 2005-08-10 2007-02-22 Ge Healthcare Bio-Sciences Corp. Improvements to protein arrays
WO2008002236A1 (en) * 2006-06-28 2008-01-03 Ge Healthcare Bio-Sciences Ab Method of detecting interactions between protein components
WO2008016912A2 (en) * 2006-07-31 2008-02-07 Case Western Reserve University Systems and methods of analyzing two dimensional gels
US8859734B2 (en) 2006-09-29 2014-10-14 Intel Corporation Method for the selective enrichment and labeling of phosphorproteins
US20080220442A1 (en) * 2006-12-06 2008-09-11 Proteinics Difference detection methods using isoelectric focusing chips
EP2164857B1 (en) * 2007-06-22 2016-10-05 The Trustees Of The University Of Pennsylvania Fluorescent labeling of transfer rna and study of protein synthesis
WO2010135382A1 (en) * 2009-05-18 2010-11-25 Brigham Young University Integrated microfluidic device for serum biomarker quantitation using either standard addition or a calibration curve
MX2009013417A (en) * 2009-12-09 2011-06-15 Itesm Covalent modification of proteins for the instantaneous visualisation thereof and subsequent characterisation through mass spectrometry.
CN112513707B (en) * 2018-04-17 2023-05-26 克莫麦特公司 Depiction of objects
CN116340723B (en) * 2023-05-22 2023-08-01 安徽中科大国祯信息科技有限责任公司 Rural water pollution quick tracing method and system based on big data

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Also Published As

Publication number Publication date
ATE283486T1 (en) 2004-12-15
WO2001011373A3 (en) 2001-07-12
CA2381506C (en) 2004-07-27
US6426190B1 (en) 2002-07-30
DE60016233T2 (en) 2005-12-08
US7566544B2 (en) 2009-07-28
JP4589587B2 (en) 2010-12-01
ES2233421T3 (en) 2005-06-16
US20020177122A1 (en) 2002-11-28
WO2001011373A2 (en) 2001-02-15
JP2003506718A (en) 2003-02-18
EP1200833B1 (en) 2004-11-24
US7598047B2 (en) 2009-10-06
AU778065B2 (en) 2004-11-11
EP1200833A1 (en) 2002-05-02
WO2001011373A8 (en) 2002-05-30
AU6534500A (en) 2001-03-05
AU2005200632A1 (en) 2005-03-10
US20040161780A1 (en) 2004-08-19
DE60016233D1 (en) 2004-12-30

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