CA2400724A1 - Porous ferro- or ferrimagnetic glass particles for isolating molecules - Google Patents

Porous ferro- or ferrimagnetic glass particles for isolating molecules Download PDF

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Publication number
CA2400724A1
CA2400724A1 CA002400724A CA2400724A CA2400724A1 CA 2400724 A1 CA2400724 A1 CA 2400724A1 CA 002400724 A CA002400724 A CA 002400724A CA 2400724 A CA2400724 A CA 2400724A CA 2400724 A1 CA2400724 A1 CA 2400724A1
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Prior art keywords
ferro
ferrimagnetic
porous
glass
particles
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CA002400724A
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French (fr)
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CA2400724C (en
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Philippe Sauer
Bernd Springer
Thomas Manz
Christoph Ritt
Roland Fabis
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Qiagen GmbH
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/005—Pretreatment specially adapted for magnetic separation
    • B03C1/01—Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y25/00—Nanomagnetism, e.g. magnetoimpedance, anisotropic magnetoresistance, giant magnetoresistance or tunneling magnetoresistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01F—MAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
    • H01F1/00—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
    • H01F1/0036—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties showing low dimensional magnetism, i.e. spin rearrangements due to a restriction of dimensions, e.g. showing giant magnetoresistivity
    • H01F1/0045—Zero dimensional, e.g. nanoparticles, soft nanoparticles for medical/biological use
    • H01F1/0054—Coated nanoparticles, e.g. nanoparticles coated with organic surfactant
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2982—Particulate matter [e.g., sphere, flake, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2982—Particulate matter [e.g., sphere, flake, etc.]
    • Y10T428/2991—Coated
    • Y10T428/2993—Silicic or refractory material containing [e.g., tungsten oxide, glass, cement, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2982—Particulate matter [e.g., sphere, flake, etc.]
    • Y10T428/2991—Coated
    • Y10T428/2993—Silicic or refractory material containing [e.g., tungsten oxide, glass, cement, etc.]
    • Y10T428/2996—Glass particles or spheres
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2982—Particulate matter [e.g., sphere, flake, etc.]
    • Y10T428/2991—Coated
    • Y10T428/2998—Coated including synthetic resin or polymer

Abstract

Porous, ferro- or ferrimagnetic, glass particles are described that selectively bind molecules of interest, especially nucleic acid molecules, under appropriate conditions. Methods of preparing the porous, ferro- or ferrimagnetic, glass particles and their use of identifying or separating molecules of interest are also described. Kits comprising the porous, ferro- or ferrimagnetic, glass particles are also provided.

Claims (41)

1. A porous, ferro-or ferrimagnetic, glass particle comprising silica glass precipitated or adsorbed on iron oxide particles or pigments, wherein the pores of the silica glass particles comprise pores having a diameter greater than and less than 10 nm, and wherein the cumulative pore area of the pores having a diameter greater than 10 nm is greater than 4 m2/g.
2. The porous, ferro-or ferrimagnetic, glass particle according to claim 1 having a diameter of about 5-25 µm.
3. The porous, ferro-or ferrimagnetic, glass particle according to claim 1 having a diameter of about 6- 15 µm.
4. The porous, ferro-or ferrimagnetic, glass particle according to claim 1 having a diameter of about 7 -10 µm.
5. The porous, ferro-or ferrimagnetic, glass particle according to claim 1, wherein the total BET surface area of the particles is greater than 190 m2/g.
6. The porous, ferro-or ferrimagnetic, glass particle according to claim 1, wherein the total BET surface area of the particle is in the range of about 190-270 m2/g.
7. The porous, ferro-or ferrimagnetic, glass particle according to claim 1, wherein the cumulative pore area of the pores having a diameter greater than 10 nm is in the range of about 4 -8 m2/g.
8. The porous, ferro-or ferrimagnetic, glass particle according to claim 1, wherein the iron oxide particle or pigment is ferrite.
9. The porous, ferro-or ferrimagnetic, glass particle according to claim 1, wherein the iron oxide particle or pigment is magnetite.
10. The porous, ferro-or ferrimagnetic, glass particle according to claim 1, wherein the iron oxide particle or pigment is a mixture of ferrite and magnetite.
11. The porous, ferro-or ferrimagnetic, glass particle according to claim 1, wherein the iron oxide particle or pigment is ferrimagnetic magnetite.
12. The porrous, ferro-or ferrimagnetic, glass particle according to claim 1, wherein the particles are about 30-50% by weight SiO2 and about 50-70 % by weight Fe3O4.
13. The porrous, ferro-or ferrimagnetie glass particle according to claim 12, wherein the particles are about 35-40% by weight SiO2 and about 55-65% by weight Fe3O4.
14. The porrous, ferro-or ferrimagnetic glass particle according to claim 1, wherein the iron oxide particles or pigments have an average diameter in the range of about 75-300 nm.
15. The porrous, ferro-or ferrimagnetic glass particle according to claim 1, wherein 90% of the iron oxide particles or pigments have a diameter in the range of about 75-300 nm.
16. The porrous, ferro-or ferrimagnetic, glass particle according to claim 1, wherein 90%
of the iron oxide particles or pigments have a diameter in the range of about 75-300 nm.
17. A kit for separating, isolating, or detecting a molecule of interest from a mixture comprising a porous, ferro-or ferrimagnetic, glass particle according to any one of claims 1-16.
18. The kit according to claim 17, wherein the molecule of interest is selected from the group consisting of nucleic acids, proteins, polypeptides, peptides, carbohydrates, lipids, and combinations thereof.
19. The kit according to claim 18, wherein the molecule of interest is a nucleic acid molecule.
20. The kit according to claims 17,18, or 19 further comprising a chaotropic agent.
21. The kit according to claim 20, wherein the chaotropic agent is selected from the group consisting of sodium perchlorate, guanidinium hydrochloride, guanidinium isothiocyanate, potassium iodide, potassium thiocyanate, sodium chloride, sodium isothiocyanate magnesium chloride, sodium iodide, and combinations thereof.
22. A method of making porous, ferro-or ferrinaagnetic, glass particles comprising:

a) providing a suspension of ferro-or ferrimagnetic iron oxide particles, b) adding a tetraalkoxysilane to the suspension of the ferro-or ferrimagnetic iron oxide particles at a pH between 6 and 8, c) hydrolyzing the tetraalkoxysilarle and precipitating silica glass on the ferro-or ferrimagnetic iron oxide particles by adding a buffer having a pH lower than 6 or higher than 8 to the suspension to form ferro-or ferrimagnetic, porous glass particles, d) separating the resulting porous, ferro- or fernmagnetic, glass particles from suspension e) washing the separated porous, ferro-or ferrimagnetic, glass particles with an anhydrous alcohol, and f) drying the porous, ferro-or ferrimagnetic, glass particles at a temperature belong the Curie temperature.
23. The method of making porous, ferro-or ferrimagnetic, glass particles according to claim 22, wherein the ferro-or ferrimagnetic iron oxide particles are provided in a suspension of glycerol or glycol.
24. The method of making the porous, ferro-or ferrirtaagnetic, glass particles according to claim 22, wherein the iron oxide particles in suspension range in size from about 75 nm to 300 nm.
25. The method of making porous, ferro-or ferrimagnetic, glass particles according to claim 22, wherein the tetraalkoxysilane corresponds to the general formula Si(OC~H2n+1)4, where n is 1,2,3,4,or5.
26. The method of making porous, ferro-or ferrimagnetic, glass particles according to claim 22, wherein the tetraalkoxysilane is tetraethoxysilane
27. The method of making ferro-or ferrimagnetic, porous, glass particles according to claim 22, wherein the tetraalkoxysilane is added to the ferro-or ferrimagnetic iron oxide particles as a solution of tetraethoxysilane (30 %) and an aliphatic C1-C4 - alcohol (70 %).
28. The method of making porous, ferro-or ferrimagnetic, glass particles according to claim 22, wherein the tetraalkoxysilane is added to the suspension of the ferro-or ferrimagnetic iron oxide particles at a pH of about 7.
29. The method of snaking porous, ferro- or ferrimagnetic, glass particles according to claim 22, wherein the tetsaalkoxysilane is added to the suspension of the ferro- or ferrimagnetic iron oxide particles within about ten minutes.
30. The method of making porous, ferro- or ferrimagnetic, glass particles according to claim 22, wherein. the buffer having a pH lower than 6 is an acetate buffer having a pH of about 5.
31. The method of making porous, ferro- or ferrimagnetic, glass particles according to claim 22, wherein the buffer having a pH greater than 9 is an ammonia/ammonium salt buffer having a pH of about 11.
32. The method of making porous, ferro- or ferrimagnetic, glass particles according to claim 22, wherein the anhydrous alcohol used to wash the particles is anhydrous ethanol.
33. The method of making the porous, ferro- or ferrimagnetic, glass particles according to claim 22, wherein the temperature at which the particles are dried is about 300 °C.
34. A method of making porous, ferro- or ferrimagnetic, glass particles comprising:

a) providing a suspension of ferro- or ferrimagnetic iron oxide particles in a glycerol solution that has a pH of 5 or less or a pH of 9 or greater, b) adding a tetraethyoxysilane dropwise with stirring to the suspension of the ferro- or ferrimagnetic iron oxide particles within 10 minutes, c) aging the mixture to allow the ferro- or ferrimagnetic porous glass particles to solidify and stabilize, d) separating the resulting porous, ferro- or ferrimagnetic, glass particles from the liquid by applying an external magnetic field, e) washing the separated porous, ferro- or ferrimagnetic, glass particles with an anhydrous alcohol, and f) drying the porous, ferro- or ferrimagnetic, glass particles at a temperature between about 300 and 500 °C.
35. ,A method of making porous, Ferro- or ferrimagnetic, glass particles comprising:

a) providing a suspension of ferro- or ferrimagnetic iron oxide particles in alcohol, preferably ethanol, b) adding silica nanoparticles to the suspension of the ferro- or ferrimagnetic iron oxide particles at a pH lower than 6, c) aging the mixture by continuous stirring, d) separating the resulting porous, ferro- or ferrimagnetic, glass particles from the liquid, e) washing the separated porous, ferro- or ferrimagnetic, glass particles. and f) drying the porous, ferro- or ferrimagnetic, glass particles at a temperature at about 200°C.
36. A method of making porous, ferro- or ferrimagnetic, glass particles comprising:

a) providing a suspension of Ferro- or ferrimagnetic iron oxide particles in paraffin oil b) adding an aquaeous sodium silicate solution, preferably 27% SiO2 in water, to the suspension of the ferro- or ferrimagnetic iron oxide particles at a pH louver than 6, c) aging the mixture by continuous stirring, d) separating the resulting porous, ferro- or ferrimagnetic, glass particles from the liquid, e) washing the separated porous, ferro- or ferrimagnetic, glass particles, and f) drying the porous, ferro- or ferrimagnetic, glass particles at a temperature at about 200°C.
37. A method of isolating a molecule of interest from a mixture, comprising:

providing a mixture containing a molecule of interest;
contacting the mixture with a porous, ferro- or ferrimagnetic, glass particle of any one of claims 1-16;

allowing the molecule of interest in the mixture to adhere to the porous, ferro- or ferrimagnetic, glass particles;

collecting the porous, ferro- or ferrirnagnetic, glass particles containing the adherent molecule of interest by applying an external magnetic field; and removing the porous, ferro- ox ferrimagnetic, glass particles with the adherent molecule of interest from the unbound components of the mixture.
38. The method of isolating a molecule of interest from a mixture according to claim 37, further comprising the step of eluting the adherent molecule of interest from the porous, ferro- or ferrimagnetic, glass particles.
39. The method of isolating a molecule of interest from a mixture according to claim 37 or claim 38, wherein the molecule of interest is selected from a the group consisting of nucleic acids, proteins, polypeptides, peptides, carbohydrates, lipids, and combinations thereof.
40. The method of isolating a molecule of interest from a mixture according to claim 39;
wherein the molecule of interest is a nucleic acid molecule,
41. The method o~isolating a nucleic acid according to claim 40, wherein the nucleic acid is selected from the group consisting of plasmid DNA, genomic DNA, cDNA, PCR DNA, linear DNA, RNA, ribozymes, aptamers, and chemically synthesized nucleic acids.
CA002400724A 2000-03-24 2001-03-22 Porous ferro- or ferrimagnetic glass particles for isolating molecules Expired - Lifetime CA2400724C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US19209300P 2000-03-24 2000-03-24
US60/192,093 2000-03-24
PCT/EP2001/003294 WO2001071732A2 (en) 2000-03-24 2001-03-22 Porous ferro- or ferrimagnetic glass particles for isolating molecules

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CA2400724A1 true CA2400724A1 (en) 2001-09-27
CA2400724C CA2400724C (en) 2009-11-24

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US (3) US7183002B2 (en)
EP (1) EP1266385B1 (en)
JP (2) JP4907030B2 (en)
AT (1) ATE419629T1 (en)
AU (2) AU2001263800B2 (en)
CA (1) CA2400724C (en)
DE (1) DE60137203D1 (en)
WO (1) WO2001071732A2 (en)

Families Citing this family (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001263800B2 (en) * 2000-03-24 2006-03-09 Qiagen Gmbh Porous ferro- or ferrimagnetic glass particles for isolating molecules
GB0116359D0 (en) * 2001-07-04 2001-08-29 Genovision As Preparation of polymer particles
US7347976B2 (en) 2001-12-20 2008-03-25 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix
US7192560B2 (en) 2001-12-20 2007-03-20 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using anion exchange
DE10201084A1 (en) * 2002-01-14 2003-07-24 Bayer Ag Magnetic particles containing silicon, process for their production and use of the particles
GB0215185D0 (en) 2002-07-01 2002-08-07 Genovision As Binding a target substance
AU2003302247A1 (en) * 2002-12-12 2004-07-09 Mykrolis Corporation Porous sintered composite materials
US7981600B2 (en) 2003-04-17 2011-07-19 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using an anion exchange material that includes a polyoxyalkylene
ATE554166T1 (en) † 2003-07-25 2012-05-15 Life Technologies Corp METHOD AND COMPOSITIONS FOR PRODUCING RNA FROM A FIXED SAMPLE
US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
EP1678147B1 (en) 2003-09-15 2012-08-08 Lead Discovery Center GmbH Pharmaceutically active 4,6-disubstituted aminopyrimidine derivatives as modulators of protein kinases
DE10355409A1 (en) * 2003-11-25 2005-06-30 Magnamedics Gmbh Spherical, magnetic silica gel carriers with increased surface area for the purification of nucleic acids
US7727710B2 (en) 2003-12-24 2010-06-01 3M Innovative Properties Company Materials, methods, and kits for reducing nonspecific binding of molecules to a surface
US7939249B2 (en) 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
EP1715953A1 (en) * 2004-02-18 2006-11-02 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
MXPA05001815A (en) * 2004-02-20 2005-08-24 Hoffmann La Roche Adsorption of nucleic acids to a solid phase.
EP1690938A1 (en) * 2005-02-11 2006-08-16 Qiagen GmbH Method for isolating nucleic acids, wherein the nucleic acids are immobilised on a matrix at elevated temperatures
JP5007920B2 (en) * 2005-08-31 2012-08-22 日立金属株式会社 Nucleic acid extraction method, cancer cell detection method and magnetic beads
JP4297148B2 (en) * 2006-09-22 2009-07-15 ソニー株式会社 Nucleic acid recovery apparatus and nucleic acid recovery method
DE102006054173A1 (en) * 2006-11-16 2008-05-21 Qiagen Gmbh Process for the preparation of magnetic silica particles
EP1995316A1 (en) 2007-05-25 2008-11-26 Qiagen GmbH Method for gentle purification of cells, cell production and cell transfection
CN101279246B (en) * 2007-12-28 2011-07-27 华侨大学 Preparation of thiophilic magnetic compound microballoons and method for separating immune globulin G using the compound microballoons
KR101053023B1 (en) * 2008-02-14 2011-08-01 (주)바이오니아 Spherical Silica Magnetic Particles and Manufacturing Method Thereof
US20090218711A1 (en) * 2008-02-28 2009-09-03 David Dasher Method of increasing ceramic paste stiffening/gelation temperature by using a salt and precursor batch
EP2128169A1 (en) 2008-05-30 2009-12-02 Qiagen GmbH Method for isolating short chain nucleic acids
DE102008026058A1 (en) 2008-05-30 2009-12-03 Qiagen Gmbh Lysis, binding and / or washing reagent useful for isolation and / or purification of nucleic acids
EP2264168B1 (en) 2009-06-18 2014-12-17 Qiagen GmbH Method for isolating nucleic acids
TWI407994B (en) 2009-10-22 2013-09-11 Ind Tech Res Inst Method, agent, and kit for isolating nucleic acids
EP2345719A1 (en) 2010-01-18 2011-07-20 Qiagen GmbH Method for isolating small RNA
RU2444011C1 (en) * 2010-07-06 2012-02-27 Учреждение Российской академии медицинских наук научно-исследовательский институт вакцин и сывороток им. И.И. Мечникова РАМН (НИИВС им. И.И. Мечникова РАМН) Method for detection of enteroviruses in water
EP2407540A1 (en) 2010-07-15 2012-01-18 Qiagen GmbH Method for purifying a target nucleic acid
DE102010034083A1 (en) * 2010-08-12 2012-02-16 Süd-Chemie AG Magnetic glass particles for use in biogas plants, fermentation and separation processes
DE102010039232B4 (en) 2010-08-12 2013-02-21 Dennert Poraver Gmbh Process for the production of magnetic expanded glass granules
CN102179230B (en) * 2011-04-01 2012-09-19 中国科学院生态环境研究中心 Preparation method of magnetized silicon dioxide aerogel
CN103764827B (en) 2011-07-04 2018-04-24 恰根有限公司 Available for separation and/or the reagent of purification of nucleic acid
EP3216877B1 (en) 2011-08-12 2020-10-21 QIAGEN GmbH Method for isolating nucleic acids
DE102012201774A1 (en) 2012-02-07 2013-08-22 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Magnetically separable microparticles with a silicate shell, process for their preparation and their use
EP2634254A1 (en) 2012-02-29 2013-09-04 QIAGEN GmbH Method for isolating nucleic acids from a food sample
CN104350152B (en) * 2012-06-01 2017-08-11 欧米伽生物技术公司 Selective kernel acid fragment is reclaimed
FR2994184B1 (en) 2012-08-02 2017-12-08 Biomerieux Sa METHODS OF FUNCTIONALIZATION AND REAGENTS USED IN SUCH METHODS USING AN AZA-ISATOIC ANHYDRIDE OR ONE OF ITS DERIVATIVES, BIOLOGIC MOLECULES SO TREATED AND KITS
WO2014029792A1 (en) 2012-08-21 2014-02-27 Qiagen Gmbh Virus particle stabilisation and method for isolating viral nucleic acids
EP2888363B1 (en) 2012-08-21 2018-06-06 Qiagen GmbH Method for isolating nucleic acids from a formaldehyde releaser stabilized sample
WO2014033326A1 (en) 2012-09-03 2014-03-06 Qiagen Gmbh Method for isolating rna including small rna with high yield
EP2931661B1 (en) 2012-12-11 2017-11-08 Qiagen GmbH Preparation of silica particles
EP2954054B1 (en) 2013-02-08 2018-12-05 Qiagen GmbH Method for separating dna by size
ES2865124T3 (en) 2013-03-15 2021-10-15 Abbott Molecular Inc One-step procedure for the purification of nucleic acids
WO2015077372A1 (en) 2013-11-19 2015-05-28 Qd Vision, Inc. Luminescent particle, materials and products including same, and methods
CN103903827B (en) * 2014-03-20 2018-01-23 哈尔滨益材新材料有限公司 A kind of preparation method and applications of magnetic silicon dioxide composite microsphere
CN103908945B (en) * 2014-03-31 2015-09-16 洛阳惠尔纳米科技有限公司 A kind of preparation method of nucleic acid extraction magnetic bead and application
CN104465008A (en) * 2014-11-18 2015-03-25 成都理想财富投资咨询有限公司 Magnetic fluid preparation method for DNA purification
FR3031063B1 (en) 2014-12-30 2017-02-10 Biomerieux Sa MULTILAYER COMPLEX, PROCESS FOR MANUFACTURING AND USE OF THE COMPLEX
EP3059312A1 (en) 2015-02-20 2016-08-24 QIAGEN GmbH Nucleic acid extraction method
KR101646610B1 (en) 2015-05-28 2016-08-09 (주)바이오니아 High active magnetic silica nano particles for separating biomaterial and method for preparing the same
US10711265B2 (en) 2015-06-01 2020-07-14 Qiagen Gmbh Electrophoresis assisted method for purifying a target nucleic acid using a delayed elution approach
WO2016193281A1 (en) 2015-06-01 2016-12-08 Qiagen Gmbh Electrophoresis assisted method and device for purifying a charged target molecule from a sample
WO2016193490A1 (en) 2015-06-05 2016-12-08 Qiagen Gmbh Method for separating dna by size
EP3307907B1 (en) 2015-06-09 2023-11-15 Biocartis NV Automatable method for nucleic acid isolation
US11827929B2 (en) 2015-07-23 2023-11-28 Biocartis, Nv Optimized clinical sample sequencing
US20180135040A1 (en) 2016-02-16 2018-05-17 Life Magnetics, Inc. Methods for separating nucleic acids with graphene coated magnetic beads
WO2018004309A1 (en) 2016-06-30 2018-01-04 김성천 Double-stranded nucleic acid signal probe and method for detecting target molecule using same
DE102016121483B4 (en) 2016-11-09 2020-06-18 Axagarius Gmbh & Co. Kg Particulate solid composite material for nucleic acid purification, containing magnetic nanoparticles, process for its production and its use
EP3555286B1 (en) 2016-12-15 2021-11-10 QIAGEN GmbH Method for isolating highly pure nucleic acid with magnetic particles
WO2019063071A1 (en) 2017-09-27 2019-04-04 Qiagen Gmbh Method for isolating rna with high yield
TR201903564A2 (en) 2019-03-08 2019-03-21 Safas Saf Plastik Sanayi Ve Ticaret Anonim Sirketi METHOD FOR PRODUCING CONTINUOUS BLOCK FLEXIBLE POLYURETHANE FOAM CONTAINING MAGNETITE PARTICULATES AS A STRESS AND PAIN REDUCER
AU2021208466A1 (en) 2020-01-13 2022-08-11 Fluent Biosciences Inc. Single cell sequencing
CA3175931A1 (en) 2020-03-16 2021-09-23 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
CA3179338A1 (en) * 2020-04-03 2021-10-07 Norwegian University Of Science And Technology (Ntnu) Methods and products for isolating nucleic acids
CN112563016A (en) * 2020-12-23 2021-03-26 四川迈克生物新材料技术有限公司 Preparation method of magnetic microspheres for nucleic acid extraction, prepared product and application
CA3209578A1 (en) * 2021-01-25 2022-07-28 Fluent Biosciences Inc. Emulsification with magnetic hydrogels
CN112891986B (en) * 2021-01-30 2022-05-03 哈尔滨工业大学 Integrated closed nucleic acid automatic extraction device
US20230139535A1 (en) * 2021-10-19 2023-05-04 Phenomenex, Inc. Systems and methods of simple and automatable protein digestion using magnetic beads

Family Cites Families (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3549524A (en) 1965-11-10 1970-12-22 Wolfgang Haller Material and method for performing steric separations
US3758284A (en) 1970-12-17 1973-09-11 W Haller Porous material and method of making the same
US4169804A (en) 1976-08-19 1979-10-02 Minnesota Mining And Manufacturing Company Magnetically responsive composite microparticle
US4126437A (en) 1976-12-02 1978-11-21 Xerox Corporation Magnetic glass carrier materials
US4335094A (en) 1979-01-26 1982-06-15 Mosbach Klaus H Magnetic polymer particles
JPS55147143A (en) * 1979-03-29 1980-11-15 Toyo Eng Corp Agitation method
US4233169A (en) * 1979-04-13 1980-11-11 Corning Glass Works Porous magnetic glass structure
US4395271A (en) 1979-04-13 1983-07-26 Corning Glass Works Method for making porous magnetic glass and crystal-containing structures
US4297337A (en) * 1979-04-13 1981-10-27 Corning Glass Works Solid-phase immunoassays using magnetic glass
FR2461521A1 (en) * 1979-07-20 1981-02-06 Anvar MAGNETIC FLUIDS, IN PARTICULAR FERROFLUIDS, AND PROCESS FOR OBTAINING THEM
EP0030086B2 (en) * 1979-11-13 1990-03-14 TECHNICON INSTRUMENTS CORPORATION (a New York corporation) Test-tube assembly, kit for making it and method of manual immunoassay
JPS5676510A (en) * 1979-11-28 1981-06-24 Tdk Corp Manufacture of magnetic recording material
US4628037A (en) * 1983-05-12 1986-12-09 Advanced Magnetics, Inc. Binding assays employing magnetic particles
US4554088A (en) * 1983-05-12 1985-11-19 Advanced Magnetics Inc. Magnetic particles for use in separations
GB8317228D0 (en) * 1983-06-24 1983-07-27 Exxon Research Engineering Co Magnetizable adsorbents
US4523996A (en) * 1984-02-22 1985-06-18 Westinghouse Electric Corp. Method of separating cationic from anionic beads in mixed resin beds
JPS6139509A (en) * 1984-07-31 1986-02-25 Japan Synthetic Rubber Co Ltd Material containing ultrafine grain ferrous oxide and manufacture thereof
DE3582649D1 (en) * 1984-11-01 1991-05-29 Technicon Instr MAGNETICALLY SENSITIVE REAGENT AND METHOD FOR THE PRODUCTION THEREOF.
US5512332A (en) 1985-10-04 1996-04-30 Immunivest Corporation Process of making resuspendable coated magnetic particles
US4988618A (en) * 1987-11-16 1991-01-29 Gene-Trak Systems Magnetic separation device and methods for use in heterogeneous assays
US4895650A (en) * 1988-02-25 1990-01-23 Gen-Probe Incorporated Magnetic separation rack for diagnostic assays
EP0343934B1 (en) * 1988-05-24 1995-01-25 Anagen (U.K.) Limited Magnetically attractable particles and method of preparation
NL8900725A (en) 1989-03-23 1990-10-16 Az Univ Amsterdam METHOD AND COMBINATION OF AGENTS FOR INSULATING NUCLEIC ACID.
US5523231A (en) 1990-02-13 1996-06-04 Amersham International Plc Method to isolate macromolecules using magnetically attractable beads which do not specifically bind the macromolecules
GB9003253D0 (en) 1990-02-13 1990-04-11 Amersham Int Plc Precipitating polymers
US5610274A (en) 1991-11-20 1997-03-11 Cpg, Inc. Production and use of magnetic porous inorganic materials
US5734020A (en) * 1991-11-20 1998-03-31 Cpg, Inc. Production and use of magnetic porous inorganic materials
GB9223334D0 (en) 1992-11-06 1992-12-23 Hybaid Ltd Magnetic solid phase supports
DE4307262A1 (en) 1993-03-02 1994-09-08 Christian Bergemann Magnetic polymeric silicon dioxide
DE4321904B4 (en) 1993-07-01 2013-05-16 Qiagen Gmbh Method for chromatographic purification and separation of nucleic acid mixtures
JPH07235407A (en) * 1994-02-22 1995-09-05 Hitachi Ltd Magnetic particle
GB9411572D0 (en) 1994-06-09 1994-08-03 Amersham Int Plc Magnetic bead precipitation method
DE19520398B4 (en) * 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetic pigment
JP2965131B2 (en) 1995-07-07 1999-10-18 東洋紡績株式会社 Magnetic carrier for nucleic acid binding and nucleic acid isolation method using the same
US5904848A (en) 1996-02-21 1999-05-18 Cpg, Inc. Controlled pore glass-synthetic resin membrane
JPH1081507A (en) * 1996-09-05 1998-03-31 Mizusawa Ind Chem Ltd Magnetic substance-amorphous silica composite particle and its production
DE19638591A1 (en) 1996-09-20 1998-04-02 Merck Patent Gmbh Spherical magnetic particles
HU225261B1 (en) * 1997-01-21 2006-08-28 Grace W R & Co Silica adsorbent on magnetic substrate
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
JP3735990B2 (en) * 1997-01-28 2006-01-18 東ソー株式会社 Method for producing magnetic silica gel
WO1998051435A1 (en) 1997-05-14 1998-11-19 Weixin Tang Fully-coated, uniform-sized metallic particles
JPH11262387A (en) * 1998-03-16 1999-09-28 Toyobo Co Ltd Nucleic acid-binding magnetic carrier and isolation of nucleic acid using the carrier
US20020104801A1 (en) * 1998-04-06 2002-08-08 Nicolas Voute Small dense microporous solid support materials, their preparation,and use for purification of large macromolecules and bioparticles
GB9812800D0 (en) 1998-06-12 1998-08-12 Rolic Ag Optically active compounds
JP2000256388A (en) * 1999-03-10 2000-09-19 Jsr Corp Magnetic silica particle for nucleic acid binding and isolation of nucleic acid
US6296934B1 (en) * 1999-03-12 2001-10-02 E.I. Du Pont De Nemours And Company Glitter containing filaments for use in brushes
AU2001263800B2 (en) * 2000-03-24 2006-03-09 Qiagen Gmbh Porous ferro- or ferrimagnetic glass particles for isolating molecules
US6607667B2 (en) * 2000-11-28 2003-08-19 W. R. Grace & Co.-Conn. Method for adsorbing substances using silica adsorbent on magnetic substrate

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