CA2413976A1 - Biosensor apparatus and method with sample type and volume detection - Google Patents
Biosensor apparatus and method with sample type and volume detection Download PDFInfo
- Publication number
- CA2413976A1 CA2413976A1 CA002413976A CA2413976A CA2413976A1 CA 2413976 A1 CA2413976 A1 CA 2413976A1 CA 002413976 A CA002413976 A CA 002413976A CA 2413976 A CA2413976 A CA 2413976A CA 2413976 A1 CA2413976 A1 CA 2413976A1
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- CA
- Canada
- Prior art keywords
- biosensor
- sample
- biosensor cell
- signal
- effective capacitance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3273—Devices therefor, e.g. test element readers, circuitry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R27/00—Arrangements for measuring resistance, reactance, impedance, or electric characteristics derived therefrom
- G01R27/02—Measuring real or complex resistance, reactance, impedance, or other two-pole characteristics derived therefrom, e.g. time constant
- G01R27/26—Measuring inductance or capacitance; Measuring quality factor, e.g. by using the resonance method; Measuring loss factor; Measuring dielectric constants ; Measuring impedance or related variables
- G01R27/2605—Measuring capacitance
Abstract
A biosensor apparatus and method with sample type and cell volume detection.
The apparatus includes a sine wave generator to apply an AC signal to a biosensor cell containing a sample, a current-to-voltage converter, a phase shifter, a square wave generator, a synchronous demodulator, and a low pass filter which yields a signal proportional to the effective capacitance across the biosensor cell, which is proportional to the volume of the sample. In addition, the current-to-voltage converter yields a signal indicative of the type of sample contained within the biosensor cell. The method includes applying a sine wave to the biosensor cell, shifting the phase of the resultant signal, generating a square wave synchronous with the sine wave, demodulating the resultant signal with the square wave, and filtering the demodulated signal to produce a signal proportional to the effective capacitance across the biosensor cell. The biosensor apparatus and method are capable of determining sample type and measuring glucose levels over a wide range of sample volumes.
The apparatus includes a sine wave generator to apply an AC signal to a biosensor cell containing a sample, a current-to-voltage converter, a phase shifter, a square wave generator, a synchronous demodulator, and a low pass filter which yields a signal proportional to the effective capacitance across the biosensor cell, which is proportional to the volume of the sample. In addition, the current-to-voltage converter yields a signal indicative of the type of sample contained within the biosensor cell. The method includes applying a sine wave to the biosensor cell, shifting the phase of the resultant signal, generating a square wave synchronous with the sine wave, demodulating the resultant signal with the square wave, and filtering the demodulated signal to produce a signal proportional to the effective capacitance across the biosensor cell. The biosensor apparatus and method are capable of determining sample type and measuring glucose levels over a wide range of sample volumes.
Claims (28)
1. An apparatus for measuring the effective capacitance across a biosensor cell having a first conductor connection and a second conductor connection, the biosensor cell configured to receive a sample having a volume, said apparatus comprising:
a sine wave generator having an output for coupling to the first conductor connection of the biosensor cell, said sine wave generator producing an AC signal;
a current-to-voltage (I/V) converter having an input for coupling to the second conductor connection of the biosensor cell and further having an output;
a phase shifter having an input coupled to the output of said I/V converter and further having an output;
a square wave generator producing a square wave synchronous with said AC
signal;
a synchronous demodulator having an output, a first input coupled to said phase shifter, and a second input coupled to said square wave generator; and a low pass filter (LPF) having an input coupled to the output of said synchronous demodulator, said LPF producing a signal at an output proportional to an effective capacitance across the biosensor cell.
a sine wave generator having an output for coupling to the first conductor connection of the biosensor cell, said sine wave generator producing an AC signal;
a current-to-voltage (I/V) converter having an input for coupling to the second conductor connection of the biosensor cell and further having an output;
a phase shifter having an input coupled to the output of said I/V converter and further having an output;
a square wave generator producing a square wave synchronous with said AC
signal;
a synchronous demodulator having an output, a first input coupled to said phase shifter, and a second input coupled to said square wave generator; and a low pass filter (LPF) having an input coupled to the output of said synchronous demodulator, said LPF producing a signal at an output proportional to an effective capacitance across the biosensor cell.
2. The apparatus of claim 1, further comprising:
a DC voltage source coupled to the first conductor connection of the biosensor cell, said DC voltage source adding a DC component to said first conductor.
a DC voltage source coupled to the first conductor connection of the biosensor cell, said DC voltage source adding a DC component to said first conductor.
3. The apparatus of claim 2, wherein said phase shifter shifts the phase of a signal out of said I/V converter and removes said DC component from said signal out of said I/V
converter.
converter.
4. The apparatus of claims 1, 2 or 3, further comprising:
an analog-to-digital (AID) converter having an analog input coupled to the output of said LPF and further having a digital output, said A/D converter converting said signal proportional to the effective capacitance across the biosensor cell from analog to digital.
an analog-to-digital (AID) converter having an analog input coupled to the output of said LPF and further having a digital output, said A/D converter converting said signal proportional to the effective capacitance across the biosensor cell from analog to digital.
5. The apparatus of claim 4, further comprising:
a processor coupled to the digital output of said A/D converter to process said digital signal proportional to the effective capacitance across the biosensor cell to derive the effective capacitance across the biosensor cell.
a processor coupled to the digital output of said A/D converter to process said digital signal proportional to the effective capacitance across the biosensor cell to derive the effective capacitance across the biosensor cell.
6. The apparatus of claim 5, said processor further deriving the volume of the sample based on the effective capacitance across the biosensor cell.
7. The apparatus of claims 1 to 6, wherein said sine wave generator generates a synthesized sine wave.
8. The apparatus of claim 7, wherein said synthesized sine wave is a stair type sine wave.
9. A biosensor for measuring components within a sample, said biosensor comprising:
an apparatus according to any of the preceding claims.
an apparatus according to any of the preceding claims.
10. A method for measuring the effective capacitance across a biosensor cell having a first conductor connection and a second conductor connection, the biosensor cell configured for use in a biosensor to receive a sample having a volume, said method comprising:
Employing said apparatus of claims 1 to 8 to measure the effective capacitance gross said biosensor cell.
Employing said apparatus of claims 1 to 8 to measure the effective capacitance gross said biosensor cell.
11. The method according to claim 10, wherein said method is a method of measuring glucose.
12. The biosensor of claim 9, further comprising:
a DC voltage source coupled to the first conductor of said biosensor cell, said DC
voltage source adding a DC component to the apparatus.
a DC voltage source coupled to the first conductor of said biosensor cell, said DC
voltage source adding a DC component to the apparatus.
13. The biosensor of claim 12, wherein said phase shifter shifts the phase of a signal out of said I/V converter and removes said DC component.
14. The biosensor of claim 12, further comprising:
an analog-to-digital (A/D) converter having a digital output and an analog input, said analog input coupled to the output of the I/V converter through a first switch and coupled to the output of said LPF through a second switch, only one of said switches being closed at a time.
an analog-to-digital (A/D) converter having a digital output and an analog input, said analog input coupled to the output of the I/V converter through a first switch and coupled to the output of said LPF through a second switch, only one of said switches being closed at a time.
15. The biosensor of claim 14, further comprising:
a filter coupled between the I/V converter and the A/D converter in a path containing said first switch to remove an AC component from said path.
a filter coupled between the I/V converter and the A/D converter in a path containing said first switch to remove an AC component from said path.
16. The biosensor of claim 14, further comprising:
a processor coupled to the digital output of said A/D converter to process said digital signal proportional to the effective capacitance across the biosensor cell when said second switch is closed and to process the output of said I/V converter to determine if the sample is of a specific type when said first switch is closed.
a processor coupled to the digital output of said A/D converter to process said digital signal proportional to the effective capacitance across the biosensor cell when said second switch is closed and to process the output of said I/V converter to determine if the sample is of a specific type when said first switch is closed.
17. The biosensor of claim 14, wherein the sample is blood and the component is glucose, and wherein said processor further calculates the glucose level for the sample of blood using the derived volume.
18. The biosensor of claim 17, wherein said DC voltage source is configured to apply a first voltage of a first polarity to said biosensor cell during a first period and to apply a second DC voltage of a second polarity to said biosensor cell during a second period.
19. The biosensor of claim 18, further comprising:
a processor coupled to the digital output of said A/D converter to process said digital signal proportional to the effective capacitance across the biosensor cell when said second switch is closed, to process the output of said I/V converter when said first switch is closed to determine a first current through said biosensor cell during said first period and a second current through said biosensor cell during said second period, and determine a glucose level for said sample of blood based on said effective capacitance, said first current, and said second current.
a processor coupled to the digital output of said A/D converter to process said digital signal proportional to the effective capacitance across the biosensor cell when said second switch is closed, to process the output of said I/V converter when said first switch is closed to determine a first current through said biosensor cell during said first period and a second current through said biosensor cell during said second period, and determine a glucose level for said sample of blood based on said effective capacitance, said first current, and said second current.
20. A method for measuring the effective capacitance across a biosensor cell having a first conductor connection and a second conductor connection, the biosensor cell configured for use in a biosensor to receive a sample having a volume, said method comprising the steps of:
applying a sine wave having a determined frequency to the first conductor connection of the biosensor cell to produce an AC signal;
shifting the phase of said AC signal;
generating a square wave synchronous with said sine wave;
demodulating said AC signal with said square wave to produce a demodulated signal;
and filtering said demodulated signal to produce a signal proportional to the effective capacitance across the biosensor cell.
applying a sine wave having a determined frequency to the first conductor connection of the biosensor cell to produce an AC signal;
shifting the phase of said AC signal;
generating a square wave synchronous with said sine wave;
demodulating said AC signal with said square wave to produce a demodulated signal;
and filtering said demodulated signal to produce a signal proportional to the effective capacitance across the biosensor cell.
21. The method of claim 20, further comprising the steps of:
applying a DC voltage to the first conductor connection of the biosensor cell, said DC
voltage introducing a DC component; and removing said DC component prior to demodulating said AC signal.
applying a DC voltage to the first conductor connection of the biosensor cell, said DC
voltage introducing a DC component; and removing said DC component prior to demodulating said AC signal.
22. The method of claim 20, further comprising the step of:
converting said signal proportional to the effective capacitance of the biosensor cell from analog to digital.
converting said signal proportional to the effective capacitance of the biosensor cell from analog to digital.
23. The method of claim 20, further comprising the step of processing said signal proportional to the effective capacitance of the biosensor cell to determine the volume of the sample received by the biosensor cell.
24. The method of claim 20, further comprising the step of calibrating the biosensor.
25. The method of claim 24, wherein said calibrating step comprises the steps of obtaining a first signal average for said digital signal proportional to the effective capacitance of the biosensor cell when the sample is not received by the biosensor cell;
obtaining a second signal average when a known capacitance is coupled across the biosensor cell;
calculating a capacitance conversion slope by dividing the value of said reference capacitor by the difference between the first signal average and the second signal average;
and storing said capacitance conversion slope for correcting the signal proportional to the capacitance across the biosensor cell.
obtaining a second signal average when a known capacitance is coupled across the biosensor cell;
calculating a capacitance conversion slope by dividing the value of said reference capacitor by the difference between the first signal average and the second signal average;
and storing said capacitance conversion slope for correcting the signal proportional to the capacitance across the biosensor cell.
26. A glucose measurement method for use with a biosensor, said biosensor configured to receive a sample having a volume, said method comprising the steps of determining the volume of the sample through synchronous demodulation when the sample is received; and determining a glucose measurement for the sample if the volume is greater than a first level based on the determined volume of the sample.
27. The method of claim 26, further comprising the step of detecting whether the sample is of a specific type; and storing the glucose measurement if the sample is said specific type.
28. A method for measuring glucose levels in a sample partially filling.a biosensor cell, the biosensor cell having a filled effective capacitance, said method comprising the steps of:
determining a partially filled effective capacitance for the sample;
determining a first current level passing through the biosensor cell during a first pulse;
determining a second current level passing through the biosensor cell during a steady state portion of a second pulse;
multiplying said first current level by the ratio of the filled effective capacitance to said partially filled effective capacitance to generate a first compensated current level;
multiplying said second current level by the ratio of the filled effective capacitance to said partially filled effective capacitance to generate a second compensated current level; and calculating a glucose level for the partially filled sample based on said first and second compensated current levels.
determining a partially filled effective capacitance for the sample;
determining a first current level passing through the biosensor cell during a first pulse;
determining a second current level passing through the biosensor cell during a steady state portion of a second pulse;
multiplying said first current level by the ratio of the filled effective capacitance to said partially filled effective capacitance to generate a first compensated current level;
multiplying said second current level by the ratio of the filled effective capacitance to said partially filled effective capacitance to generate a second compensated current level; and calculating a glucose level for the partially filled sample based on said first and second compensated current levels.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/020,169 US6856125B2 (en) | 2001-12-12 | 2001-12-12 | Biosensor apparatus and method with sample type and volume detection |
| US10/020,169 | 2001-12-12 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2413976A1 true CA2413976A1 (en) | 2003-06-12 |
| CA2413976C CA2413976C (en) | 2010-06-29 |
Family
ID=21797115
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2413976A Expired - Fee Related CA2413976C (en) | 2001-12-12 | 2002-12-11 | Biosensor apparatus and method with sample type and volume detection |
Country Status (19)
| Country | Link |
|---|---|
| US (3) | US6856125B2 (en) |
| EP (2) | EP1605253A1 (en) |
| JP (1) | JP4302396B2 (en) |
| KR (1) | KR100786983B1 (en) |
| CN (1) | CN1273826C (en) |
| AT (1) | ATE309531T1 (en) |
| AU (1) | AU2002304121B2 (en) |
| CA (1) | CA2413976C (en) |
| DE (1) | DE60207185T2 (en) |
| DK (1) | DK1324025T3 (en) |
| ES (1) | ES2250593T3 (en) |
| HK (1) | HK1053511A1 (en) |
| IL (1) | IL153210A0 (en) |
| MX (1) | MXPA02011972A (en) |
| NO (1) | NO324175B1 (en) |
| PL (1) | PL357711A1 (en) |
| RU (1) | RU2307350C2 (en) |
| SG (1) | SG112863A1 (en) |
| TW (1) | TWI305265B (en) |
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| US20030109798A1 (en) | 2003-06-12 |
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