CA2415494A1 - Method of detection by enhancement of silver staining - Google Patents

Method of detection by enhancement of silver staining Download PDF

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Publication number
CA2415494A1
CA2415494A1 CA002415494A CA2415494A CA2415494A1 CA 2415494 A1 CA2415494 A1 CA 2415494A1 CA 002415494 A CA002415494 A CA 002415494A CA 2415494 A CA2415494 A CA 2415494A CA 2415494 A1 CA2415494 A1 CA 2415494A1
Authority
CA
Canada
Prior art keywords
detection
enhancement
silver staining
silver
reporter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002415494A
Other languages
French (fr)
Other versions
CA2415494C (en
Inventor
Robert L. Letsinger
Viswanadham Garimella
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Northwestern University
Original Assignee
Northwestern University
Robert L. Letsinger
Viswanadham Garimella
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Northwestern University, Robert L. Letsinger, Viswanadham Garimella filed Critical Northwestern University
Publication of CA2415494A1 publication Critical patent/CA2415494A1/en
Application granted granted Critical
Publication of CA2415494C publication Critical patent/CA2415494C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/137—Metal/ion, e.g. metal label
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/924—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis using nanostructure as support of dna analysis

Abstract

The present invention relates to a method for amplifying a detection signal by enhancing or promoting the deposition of additional silver in assay detection systems where the formation of a silver spot serves as a reporter for the presence of a target molecule, including biological polymers (e.g., proteins and nucleic acids) and small molecules.
CA002415494A 2000-07-11 2001-07-11 Method of detection by enhancement of silver staining Expired - Lifetime CA2415494C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US21778200P 2000-07-11 2000-07-11
US60/217,782 2000-07-11
PCT/US2001/021846 WO2002004681A2 (en) 2000-07-11 2001-07-11 Method of detection by enhancement of silver staining

Publications (2)

Publication Number Publication Date
CA2415494A1 true CA2415494A1 (en) 2002-01-17
CA2415494C CA2415494C (en) 2007-09-18

Family

ID=22812486

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002415494A Expired - Lifetime CA2415494C (en) 2000-07-11 2001-07-11 Method of detection by enhancement of silver staining

Country Status (8)

Country Link
US (2) US6602669B2 (en)
EP (1) EP1360318B1 (en)
JP (1) JP3605607B2 (en)
AT (1) ATE324465T1 (en)
AU (2) AU2001276870B2 (en)
CA (1) CA2415494C (en)
DE (1) DE60119170T2 (en)
WO (1) WO2002004681A2 (en)

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WO2002004681A3 (en) 2003-08-07
US20020034756A1 (en) 2002-03-21
EP1360318A2 (en) 2003-11-12
AU7687001A (en) 2002-01-21
WO2002004681A2 (en) 2002-01-17
CA2415494C (en) 2007-09-18
ATE324465T1 (en) 2006-05-15
US20040101889A1 (en) 2004-05-27
EP1360318B1 (en) 2006-04-26
JP3605607B2 (en) 2004-12-22
US6602669B2 (en) 2003-08-05
JP2004502950A (en) 2004-01-29
AU2001276870B2 (en) 2006-07-20
DE60119170T2 (en) 2007-05-24

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