CA2432463A1 - Methods for avoiding contamination of negative sample material with analyte-containing samples when using pipetting robots - Google Patents
Methods for avoiding contamination of negative sample material with analyte-containing samples when using pipetting robots Download PDFInfo
- Publication number
- CA2432463A1 CA2432463A1 CA002432463A CA2432463A CA2432463A1 CA 2432463 A1 CA2432463 A1 CA 2432463A1 CA 002432463 A CA002432463 A CA 002432463A CA 2432463 A CA2432463 A CA 2432463A CA 2432463 A1 CA2432463 A1 CA 2432463A1
- Authority
- CA
- Canada
- Prior art keywords
- reaction vessels
- lws
- liquid waste
- waste station
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
- G01N35/1016—Control of the volume dispensed or introduced
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00594—Quality control, including calibration or testing of components of the analyser
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00277—Special precautions to avoid contamination (e.g. enclosures, glove- boxes, sealed sample carriers, disposal of contaminated material)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/11—Automated chemical analysis
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/12—Condition responsive control
Abstract
The invention relates to a method for reducing the contamination of reaction containers which are empty or already fitted with analyte-free sampling material or sampling material having a low analyte concentration, inside a configuration of reaction containers which are spatially close to each other , when pipetting samples or reagents using an automatic sample distributor. Th e invention also relates to hardware-related measures for reducing or avoiding possible contamination.
Claims (10)
1. A method for reducing the contamination of empty reaction vessels, or of reaction vessels which are already charged with analyte-free sample material or sample material which contains a low concentration of analytic, within an arrangement of reaction vessels which are located in spatial proximity to each other while samples or reagents are pipetted using an automated sample dispenser, which comprises using an enzyme/substrate dye test to initially establish the extent of the contamination by means of model A:
a) adding an enzyme solution to reaction vessels in a constituent region of said spatial arrangement while the remaining reaction vessels are covered with an absorbent stratiform material which contains a suitable chromogen/substrate reagent, with a color reaction being induced by its contact with the enzyme, determining the number, intensity and/or distribution of the covered reaction vessels which are marked by a color development and subsequently modifying the liquid uptake (aspirate profile) and liquid release (dispense profile) such that the number or intensity of the reaction vessels marked by the color development is reduced.
a) adding an enzyme solution to reaction vessels in a constituent region of said spatial arrangement while the remaining reaction vessels are covered with an absorbent stratiform material which contains a suitable chromogen/substrate reagent, with a color reaction being induced by its contact with the enzyme, determining the number, intensity and/or distribution of the covered reaction vessels which are marked by a color development and subsequently modifying the liquid uptake (aspirate profile) and liquid release (dispense profile) such that the number or intensity of the reaction vessels marked by the color development is reduced.
2. The method for reducing the contamination of empty reaction vessels, or of reaction vessels which are already charged with analyte-free sample material or sample material which contains a low concentration of analytic, within an arrangement of reaction vessels which are located in spatial proximity to each other while samples or reagents are pipetted using an automated sample dispenser, which comprises using an enzyme/dye test to initially establish the extent of the contamination by means of model B:
a) adding an enzyme solution to reaction vessels in a constituent region of said spatial arrangement, b) adding a suitable chromogen/substrate reagent, which, on contact with the enzyme, induces a color reaction, to the reaction vessels of the remaining constituent region of said spatial arrangement, c) ascertaining possible contaminations by determining the color development in the reaction vessels;
and subsequently modifying the liquid uptake (aspirate profile) and liquid release (dispense profile) such that the number or intensity of the reaction vessels marked by the color development is reduced.
a) adding an enzyme solution to reaction vessels in a constituent region of said spatial arrangement, b) adding a suitable chromogen/substrate reagent, which, on contact with the enzyme, induces a color reaction, to the reaction vessels of the remaining constituent region of said spatial arrangement, c) ascertaining possible contaminations by determining the color development in the reaction vessels;
and subsequently modifying the liquid uptake (aspirate profile) and liquid release (dispense profile) such that the number or intensity of the reaction vessels marked by the color development is reduced.
3. The method as claimed in claim 1, wherein the reaction vessels are wells in a microtitration plate.
4. The method as claimed in claim 2, wherein the reaction vessels are wells in a microtitration plate.
5. The method as claimed in claim 4, wherein:
a) the automatic sample dispenser is part of a BEP 2000 or of a fully automated machine which is essentially constructed in the same way as the BEP 2000; and b) the arrangement of reaction vessels which are located in spatial proximity to each other consists of a linear arrangement, which lies in a horizontal plane, of 4 microtitration plates in the sequence: position A, position B, position C and position D as shown in fig. 3; and c) a liquid waste station (LWS) is located in immediate proximity to position A in approximate extension of the imaginary line from position D to position A; and d) the enzyme solution is added to a constituent region of the microtitration plates in positions A to D; and e) the determination of possible contaminations includes, in particular, the contaminations emanating from the liquid waste station (LWS).
a) the automatic sample dispenser is part of a BEP 2000 or of a fully automated machine which is essentially constructed in the same way as the BEP 2000; and b) the arrangement of reaction vessels which are located in spatial proximity to each other consists of a linear arrangement, which lies in a horizontal plane, of 4 microtitration plates in the sequence: position A, position B, position C and position D as shown in fig. 3; and c) a liquid waste station (LWS) is located in immediate proximity to position A in approximate extension of the imaginary line from position D to position A; and d) the enzyme solution is added to a constituent region of the microtitration plates in positions A to D; and e) the determination of possible contaminations includes, in particular, the contaminations emanating from the liquid waste station (LWS).
6. The method as claimed in claim 5, wherein the liquid waste station (LWS) is covered by an upper covering and an aperture which is located in it is as small as possible such that, on the one hand, optimal protection against splashes from the liquid waste station (LWS) is provided but, on the other hand, it is possible to reproducibly release excess sample volume through this aperture into the liquid waste station (LWS) such that no liquid inadvertently comes into contact with the edge of the aperture in this connection.
7. The method as claimed in claim 6, wherein the liquid waste station (LWS) is fashioned from the outset such that the upper vessel wall corresponds to the covering such that a separate covering can be dispensed with while retaining the function which it provides.
8. The method as claimed in claim 6, wherein the liquid waste station (LWS) is separated off by installing a mechanical protective device between the liquid waste station and the positions of the coated microtiter plates, thereby preventing contaminations.
9. A covered liquid waste station (LWS) which is suitable for use in a method as claimed in claim 6.
10. A liquid waste station (LWS) which is suitable for use in a method as claimed in claim 7.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10064428 | 2000-12-21 | ||
DE10064428.7 | 2000-12-21 | ||
PCT/EP2001/015197 WO2002049764A1 (en) | 2000-12-21 | 2001-12-21 | Method for avoiding the contamination of negative sampling material by samples containing analytes during the use of automatic pipette machines |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2432463A1 true CA2432463A1 (en) | 2002-06-27 |
CA2432463C CA2432463C (en) | 2010-05-04 |
Family
ID=7668556
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2432463A Expired - Fee Related CA2432463C (en) | 2000-12-21 | 2001-12-21 | Methods for avoiding contamination of negative sample material with analyte-containing samples when using pipetting robots |
Country Status (8)
Country | Link |
---|---|
US (2) | US7169613B2 (en) |
EP (1) | EP1349662B1 (en) |
JP (1) | JP4301490B2 (en) |
AT (1) | ATE443570T1 (en) |
CA (1) | CA2432463C (en) |
DE (1) | DE50115123D1 (en) |
ES (1) | ES2331725T3 (en) |
WO (1) | WO2002049764A1 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1191312A3 (en) * | 2000-09-21 | 2004-12-22 | Tecan Trading AG | System and method for optimization of parameters of liquid-handling instruments |
US20120295335A1 (en) * | 2010-11-18 | 2012-11-22 | E. I. Du Pont De Nemours And Company | Prevention of contamination of nutrient feed reservoirs & feed lines in bioreactor |
CN110031641A (en) * | 2013-09-12 | 2019-07-19 | 株式会社日立高新技术 | Nozzle cleaning method and automatic analysing apparatus |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5061639A (en) * | 1989-12-06 | 1991-10-29 | E. I. Dupont De Nemours And Company | Liquid dispenser accuracy verification method |
US5610069A (en) * | 1992-03-27 | 1997-03-11 | Abbott Laboratories | Apparatus and method for washing clinical apparatus |
US5635364A (en) * | 1992-03-27 | 1997-06-03 | Abbott Laboratories | Assay verification control for an automated analytical system |
US5447838A (en) * | 1992-08-05 | 1995-09-05 | Hybritech Incorporated | Protein-dye conjugate for confirmation of correct dilution of calibrators |
US5538849A (en) | 1992-12-29 | 1996-07-23 | Toyo Boseki Kabushiki Kaisha | Apparatus for automated assay of DNA probe and method for assaying nucleic acid in sample |
US5578270A (en) | 1995-03-24 | 1996-11-26 | Becton Dickinson And Company | System for nucleic acid based diagnostic assay |
ATE250978T1 (en) | 1997-11-14 | 2003-10-15 | Gen Probe Inc | WORKING DEVICE FOR ANALYSIS |
AU5287699A (en) | 1998-07-24 | 2000-02-14 | Akzo Nobel N.V. | Method of determining the carry-over of an analyte |
-
2001
- 2001-12-21 EP EP01991877A patent/EP1349662B1/en not_active Expired - Lifetime
- 2001-12-21 DE DE50115123T patent/DE50115123D1/en not_active Expired - Lifetime
- 2001-12-21 CA CA2432463A patent/CA2432463C/en not_active Expired - Fee Related
- 2001-12-21 JP JP2002551096A patent/JP4301490B2/en not_active Expired - Fee Related
- 2001-12-21 ES ES01991877T patent/ES2331725T3/en not_active Expired - Lifetime
- 2001-12-21 AT AT01991877T patent/ATE443570T1/en not_active IP Right Cessation
- 2001-12-21 US US10/451,326 patent/US7169613B2/en not_active Expired - Fee Related
- 2001-12-21 WO PCT/EP2001/015197 patent/WO2002049764A1/en active Application Filing
-
2007
- 2007-01-17 US US11/653,898 patent/US7534617B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
JP4301490B2 (en) | 2009-07-22 |
DE50115123D1 (en) | 2009-11-05 |
US7169613B2 (en) | 2007-01-30 |
US20040063185A1 (en) | 2004-04-01 |
EP1349662B1 (en) | 2009-09-23 |
US7534617B2 (en) | 2009-05-19 |
WO2002049764A1 (en) | 2002-06-27 |
ATE443570T1 (en) | 2009-10-15 |
CA2432463C (en) | 2010-05-04 |
JP2004516464A (en) | 2004-06-03 |
EP1349662A1 (en) | 2003-10-08 |
ES2331725T3 (en) | 2010-01-14 |
US20080145909A1 (en) | 2008-06-19 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |
Effective date: 20141222 |