CA2432463A1 - Methods for avoiding contamination of negative sample material with analyte-containing samples when using pipetting robots - Google Patents

Methods for avoiding contamination of negative sample material with analyte-containing samples when using pipetting robots Download PDF

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Publication number
CA2432463A1
CA2432463A1 CA002432463A CA2432463A CA2432463A1 CA 2432463 A1 CA2432463 A1 CA 2432463A1 CA 002432463 A CA002432463 A CA 002432463A CA 2432463 A CA2432463 A CA 2432463A CA 2432463 A1 CA2432463 A1 CA 2432463A1
Authority
CA
Canada
Prior art keywords
reaction vessels
lws
liquid waste
waste station
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002432463A
Other languages
French (fr)
Other versions
CA2432463C (en
Inventor
Udo Krupka
Wolfgang Schmandt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics Products GmbH
Original Assignee
Dade Behring Marburg Gmbh
Udo Krupka
Wolfgang Schmandt
Siemens Healthcare Diagnostics Products Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dade Behring Marburg Gmbh, Udo Krupka, Wolfgang Schmandt, Siemens Healthcare Diagnostics Products Gmbh filed Critical Dade Behring Marburg Gmbh
Publication of CA2432463A1 publication Critical patent/CA2432463A1/en
Application granted granted Critical
Publication of CA2432463C publication Critical patent/CA2432463C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G01N35/1016Control of the volume dispensed or introduced
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584Control arrangements for automatic analysers
    • G01N35/00594Quality control, including calibration or testing of components of the analyser
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178Special arrangements of analysers
    • G01N2035/00277Special precautions to avoid contamination (e.g. enclosures, glove- boxes, sealed sample carriers, disposal of contaminated material)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/028Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/11Automated chemical analysis
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/12Condition responsive control

Abstract

The invention relates to a method for reducing the contamination of reaction containers which are empty or already fitted with analyte-free sampling material or sampling material having a low analyte concentration, inside a configuration of reaction containers which are spatially close to each other , when pipetting samples or reagents using an automatic sample distributor. Th e invention also relates to hardware-related measures for reducing or avoiding possible contamination.

Claims (10)

1. A method for reducing the contamination of empty reaction vessels, or of reaction vessels which are already charged with analyte-free sample material or sample material which contains a low concentration of analytic, within an arrangement of reaction vessels which are located in spatial proximity to each other while samples or reagents are pipetted using an automated sample dispenser, which comprises using an enzyme/substrate dye test to initially establish the extent of the contamination by means of model A:
a) adding an enzyme solution to reaction vessels in a constituent region of said spatial arrangement while the remaining reaction vessels are covered with an absorbent stratiform material which contains a suitable chromogen/substrate reagent, with a color reaction being induced by its contact with the enzyme, determining the number, intensity and/or distribution of the covered reaction vessels which are marked by a color development and subsequently modifying the liquid uptake (aspirate profile) and liquid release (dispense profile) such that the number or intensity of the reaction vessels marked by the color development is reduced.
2. The method for reducing the contamination of empty reaction vessels, or of reaction vessels which are already charged with analyte-free sample material or sample material which contains a low concentration of analytic, within an arrangement of reaction vessels which are located in spatial proximity to each other while samples or reagents are pipetted using an automated sample dispenser, which comprises using an enzyme/dye test to initially establish the extent of the contamination by means of model B:

a) adding an enzyme solution to reaction vessels in a constituent region of said spatial arrangement, b) adding a suitable chromogen/substrate reagent, which, on contact with the enzyme, induces a color reaction, to the reaction vessels of the remaining constituent region of said spatial arrangement, c) ascertaining possible contaminations by determining the color development in the reaction vessels;
and subsequently modifying the liquid uptake (aspirate profile) and liquid release (dispense profile) such that the number or intensity of the reaction vessels marked by the color development is reduced.
3. The method as claimed in claim 1, wherein the reaction vessels are wells in a microtitration plate.
4. The method as claimed in claim 2, wherein the reaction vessels are wells in a microtitration plate.
5. The method as claimed in claim 4, wherein:
a) the automatic sample dispenser is part of a BEP 2000 or of a fully automated machine which is essentially constructed in the same way as the BEP 2000; and b) the arrangement of reaction vessels which are located in spatial proximity to each other consists of a linear arrangement, which lies in a horizontal plane, of 4 microtitration plates in the sequence: position A, position B, position C and position D as shown in fig. 3; and c) a liquid waste station (LWS) is located in immediate proximity to position A in approximate extension of the imaginary line from position D to position A; and d) the enzyme solution is added to a constituent region of the microtitration plates in positions A to D; and e) the determination of possible contaminations includes, in particular, the contaminations emanating from the liquid waste station (LWS).
6. The method as claimed in claim 5, wherein the liquid waste station (LWS) is covered by an upper covering and an aperture which is located in it is as small as possible such that, on the one hand, optimal protection against splashes from the liquid waste station (LWS) is provided but, on the other hand, it is possible to reproducibly release excess sample volume through this aperture into the liquid waste station (LWS) such that no liquid inadvertently comes into contact with the edge of the aperture in this connection.
7. The method as claimed in claim 6, wherein the liquid waste station (LWS) is fashioned from the outset such that the upper vessel wall corresponds to the covering such that a separate covering can be dispensed with while retaining the function which it provides.
8. The method as claimed in claim 6, wherein the liquid waste station (LWS) is separated off by installing a mechanical protective device between the liquid waste station and the positions of the coated microtiter plates, thereby preventing contaminations.
9. A covered liquid waste station (LWS) which is suitable for use in a method as claimed in claim 6.
10. A liquid waste station (LWS) which is suitable for use in a method as claimed in claim 7.
CA2432463A 2000-12-21 2001-12-21 Methods for avoiding contamination of negative sample material with analyte-containing samples when using pipetting robots Expired - Fee Related CA2432463C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10064428.7 2000-12-21
DE10064428 2000-12-21
PCT/EP2001/015197 WO2002049764A1 (en) 2000-12-21 2001-12-21 Method for avoiding the contamination of negative sampling material by samples containing analytes during the use of automatic pipette machines

Publications (2)

Publication Number Publication Date
CA2432463A1 true CA2432463A1 (en) 2002-06-27
CA2432463C CA2432463C (en) 2010-05-04

Family

ID=7668556

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2432463A Expired - Fee Related CA2432463C (en) 2000-12-21 2001-12-21 Methods for avoiding contamination of negative sample material with analyte-containing samples when using pipetting robots

Country Status (8)

Country Link
US (2) US7169613B2 (en)
EP (1) EP1349662B1 (en)
JP (1) JP4301490B2 (en)
AT (1) ATE443570T1 (en)
CA (1) CA2432463C (en)
DE (1) DE50115123D1 (en)
ES (1) ES2331725T3 (en)
WO (1) WO2002049764A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1191312A3 (en) * 2000-09-21 2004-12-22 Tecan Trading AG System and method for optimization of parameters of liquid-handling instruments
US20120295335A1 (en) * 2010-11-18 2012-11-22 E. I. Du Pont De Nemours And Company Prevention of contamination of nutrient feed reservoirs & feed lines in bioreactor
EP3056911B1 (en) * 2013-09-12 2021-09-08 Hitachi High-Tech Corporation Nozzle cleaning method and automated analyzer

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5061639A (en) * 1989-12-06 1991-10-29 E. I. Dupont De Nemours And Company Liquid dispenser accuracy verification method
US5635364A (en) * 1992-03-27 1997-06-03 Abbott Laboratories Assay verification control for an automated analytical system
US5610069A (en) * 1992-03-27 1997-03-11 Abbott Laboratories Apparatus and method for washing clinical apparatus
US5447838A (en) * 1992-08-05 1995-09-05 Hybritech Incorporated Protein-dye conjugate for confirmation of correct dilution of calibrators
US5538849A (en) 1992-12-29 1996-07-23 Toyo Boseki Kabushiki Kaisha Apparatus for automated assay of DNA probe and method for assaying nucleic acid in sample
US5578270A (en) * 1995-03-24 1996-11-26 Becton Dickinson And Company System for nucleic acid based diagnostic assay
CA2594172A1 (en) * 1997-11-14 1999-05-29 Gen-Probe Incorporated Assay work station
AU5287699A (en) * 1998-07-24 2000-02-14 Akzo Nobel N.V. Method of determining the carry-over of an analyte

Also Published As

Publication number Publication date
WO2002049764A1 (en) 2002-06-27
DE50115123D1 (en) 2009-11-05
ES2331725T3 (en) 2010-01-14
JP4301490B2 (en) 2009-07-22
JP2004516464A (en) 2004-06-03
ATE443570T1 (en) 2009-10-15
US7534617B2 (en) 2009-05-19
US20080145909A1 (en) 2008-06-19
EP1349662A1 (en) 2003-10-08
US20040063185A1 (en) 2004-04-01
CA2432463C (en) 2010-05-04
EP1349662B1 (en) 2009-09-23
US7169613B2 (en) 2007-01-30

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Effective date: 20141222