CA2435301C - Multi-arm block copolymers as drug delivery vehicles - Google Patents

Multi-arm block copolymers as drug delivery vehicles Download PDF

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CA2435301C
CA2435301C CA2435301A CA2435301A CA2435301C CA 2435301 C CA2435301 C CA 2435301C CA 2435301 A CA2435301 A CA 2435301A CA 2435301 A CA2435301 A CA 2435301A CA 2435301 C CA2435301 C CA 2435301C
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pharmaceutical composition
arm
polymer segment
hydrophobic
copolymer
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CA2435301A1 (en
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Xuan Zhao
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Nektar Therapeutics
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
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    • C08G63/00Macromolecular compounds obtained by reactions forming a carboxylic ester link in the main chain of the macromolecule
    • C08G63/91Polymers modified by chemical after-treatment
    • C08G63/914Polymers modified by chemical after-treatment derived from polycarboxylic acids and polyhydroxy compounds
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/70Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
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    • A61K31/724Cyclodextrins
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    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K31/74Synthetic polymeric materials
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/59Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
    • A61K47/593Polyesters, e.g. PLGA or polylactide-co-glycolide
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/59Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
    • A61K47/60Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6949Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit inclusion complexes, e.g. clathrates, cavitates or fullerenes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B82NANOTECHNOLOGY
    • B82YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
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    • C08GMACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G65/00Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
    • C08G65/02Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from cyclic ethers by opening of the heterocyclic ring
    • C08G65/26Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from cyclic ethers by opening of the heterocyclic ring from cyclic ethers and other compounds
    • C08G65/2603Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from cyclic ethers by opening of the heterocyclic ring from cyclic ethers and other compounds the other compounds containing oxygen
    • C08G65/2606Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from cyclic ethers by opening of the heterocyclic ring from cyclic ethers and other compounds the other compounds containing oxygen containing hydroxyl groups
    • C08G65/2609Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from cyclic ethers by opening of the heterocyclic ring from cyclic ethers and other compounds the other compounds containing oxygen containing hydroxyl groups containing aliphatic hydroxyl groups
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    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08GMACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G81/00Macromolecular compounds obtained by interreacting polymers in the absence of monomers, e.g. block polymers
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08LCOMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L71/00Compositions of polyethers obtained by reactions forming an ether link in the main chain; Compositions of derivatives of such polymers
    • C08L71/02Polyalkylene oxides
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08GMACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G2650/00Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
    • C08G2650/22Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule characterised by the initiator used in polymerisation
    • C08G2650/24Polymeric initiators
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    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08LCOMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L2205/00Polymer mixtures characterised by other features
    • C08L2205/05Polymer mixtures characterised by other features containing polymer components which can react with one another

Abstract

The invention provides multi-arm block copolymers useful as drug delivery vehicles comprising a central core molecule, such as a residue of a polyol, and at least three copolymer arms covalently attached to the central core molecule, each copolymer arm comprising an inner hydrophobic polymer segment covalently attached to the central core molecule and an outer hydrophilic polymer segment covalently attached to the hydrophobic polymer segment, wherein the central core molecule and the hydrophobic polymer segment define a hydrophobic core region. The solubility of hydrophobic biologically active agents can be improved by entrapment within the hydrophobic core region of the block copolymer. The invention further includes pharmaceutical compositions including such block copolymers, methods of making such copolymers and pharmaceutical compositions, and methods of using the block copolymers as drug delivery vehicles.

Description

MULTI-ARM BLOCK COPOLYMERS AS DRUG DELIVERY VEHICLES
FIELD OF THE INVENTION
The invention relates to multi-arm copolymers containing a hydrophobic core region and a hydrophilic outer region, methods of making such copolymers, and methods of using such copolymers as drug delivery vehicles.

BACKGROUND OF THE INVENTION
Solubilization and delivery of hydrophobic drugs is one of the most challenging issues in pharmaceutical formulation, particularly since most drugs are hydrophobic. Such drugs tend to precipitate in an aqueous environment, such as the bloodstream. Whether the drug is delivered by oral or parenteral routes, a certain level of aqueous solubility is required for adequate absorption and bioavailability.
Pharmaceutical grade surfactants, such as Tween 80 or Cremophor , have been widely used in formulations to compensate for the low aqueous solubility of hydrophobic drugs. These surfactants solubilize hydrophobic drugs by forming micellar structures in aqueous media. Unfortunately, these surfactants have been associated with severe allergic reactions and hypersensitivity when administered to patients (Kris, et al., Cancer Treatment REP, 70:5, (1986)). After parenteral administration, these micellar drug carriers disintegrate when the concentration is below their critical micelle concentration (CMC), resulting in a rapid release of the drug. That is to say, in addition to the possibility of adverse side effects upon administration, conventional surfactant-based carriers also lack the ability to provide controlled release of a drug.
Thus, there remains a need in the art for a method for imparting adequate levels of aqueous solubility to a hydrophobic drug such that the drug may be administered in a therapeutically effective manner.

SUMMARY OF THE INVENTION
The invention is directed to multi-arm block copolymers useful as drug delivery vehicles. The multi-arm block copolymers comprise a central core molecule, such as a residue of a polyol, and at least three copolymer arms covalently attached to the central core molecule, each copolymer arm comprising an inner hydrophobic polymer segment covalently attached to the central core molecule and an outer hydrophilic polymer segment covalently attached to the hydrophobic polymer segment. The block copolymer provides a unimolecular micelle structure, wherein the central core molecule and the hydrophobic polymer segment define a hydrophobic core region and the hydrophilic polymer segment defines an outer hydrophilic region.
The solubility of hydrophobic biologically active agents can be improved by entrapment within the hydrophobic core region of the block copolymer. Thus, improved delivery of hydrophobic drugs can be obtained by administering a pharmaceutical composition to a mammal, the pharmaceutical composition comprising a multi-arm block copolymer of the invention having a drug entrapped within the hydrophobic core region thereof.

BRIEF DESCRIPTION OF THE DRAWINGS
Having thus described the invention in general terms, reference will now be made to the accompanying figures, wherein:
Figure 1 is an illustration of the structure of an embodiment of the multi-arm block copolymer of the invention;
Figure 2 provides release profiles for the drug, 3,4-di-[1-methyl 6-nitro-3-indolyl]-1H-pyrrole-2,5-dione (MNIPD), in several polymer compositions;
Figure 3 provides release profiles for the drug, simvastatin, in several polymer compositions;
Figure 4 provides a release profile for simvastatin in an exemplary bisphosphonate derivative of a multi-arm block copolymer;
Figure 5 provides release profiles for the drug, paclitaxel, in two multi-arm block copolymer embodiments of the invention;
Figure 6 provides release profiles for the drug, indomethacin, in several polymer compositions;
Figure 7 provides release profiles for the drug, pivaloxymethyl butyrate, in two multi-arm block copolymer embodiments of the invention;
Figure 8 provides a release profile for the drug, cyclosporin A, in a multi-arm block copolymer embodiment of the invention;
Figure 9 provides a release profile for the drug, paclitaxel, in a multi-arm block copolymer embodiment of the invention;
Figure 10 provides a comparison of the in vivo effect of a conventional Taxol formulation versus an 8-arm poly(lactide)-mPEG block copolymer/Taxol formulation of the invention on lung tumor growth; and Figure 11 is an example of Dynamic Light Scattering (DLS) data.
DETAILED DESCRIPTION OF THE INVENTION
The present invention now will be described more fully hereinafter. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein; rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.
1. Definitions The terms "functional group", "active moiety", "activating group", "reactive site", "chemically reactive group" and " chemically reactive moiety" are used in the art and herein to refer to distinct, definable portions or units of a molecule. The terms are somewhat synonymous in the chemical arts and are used herein to indicate the portions of molecules that perform some function or activity and are reactive with other molecules. The term "active," when used in conjunction with functional groups, is intended to include those functional groups that react readily with electrophilic or nucleophilic groups on other molecules, in contrast to those groups that require strong catalysts or highly impractical reaction conditions in order to react (i.e., "non-reactive" or "inert" groups). For example, as would be understood in the art, the term "active ester" would include those esters that react readily with nucleophilic groups such as amines. Exemplary active esters include N-hydroxysuccinimidyl esters or 1-benzotriazolyl esters. Typically, an active ester will react with an amine in aqueous medium in a matter of minutes, whereas certain esters, such as methyl or ethyl esters, require a strong catalyst in order to react with a nucleophilic group.
The term "linkage" or "linker" is used herein to refer to an atom, groups of atoms, or bonds that are normally formed as the result of a chemical reaction.
A
linker of the invention typically links the connecting moieties, such two polymer segments, via one or more covalent bonds. Hydrolytically stable linkages means that the linkages are substantially stable in water and do not react to any significant degree with water at useful pHs, e.g., under physiological conditions for an extended period of time, perhaps even indefinitely. Hydrolytically unstable or degradable linkages means that the linkages are degradable in water or in aqueous solutions, including for example, blood. Enzymatically unstable or degradable linkages means that the linkage can be degraded by one or more enzymes.
The term "alkyl" refers to hydrocarbon chains typically ranging from about 1 to about 12 carbon atoms in length, and includes straight and branched chains.
The hydrocarbon chains may be saturated or unsaturated. The term "substituted alkyl"
refers to an alkyl group substituted with one or more non-interfering substituents, such as, but not limited to, C3-C6 cycloalkyl, e.g., cyclopropyl, cyclobutyl, and the like; acetylene; cyano; alkoxy, e.g., methoxy, ethoxy, and the like; lower alkanoyloxy, e.g., acetoxy; hydroxy; carboxyl; amino; lower alkylamino, e.g., methylamino;
ketone; halo, e.g. chloro or bromo; phenyl; substituted phenyl, and the like.
"Alkoxy" refers to an -O-R group, wherein R is alkyl or substituted alkyl, preferably C1-C6 alkyl (e.g., methoxy or ethoxy).
"Aryl" means one or more aromatic rings, each of 5 or 6 core carbon atoms.
Multiple aryl rings may be fused, as in naphthyl or unfused, as in biphenyl.
Aryl rings may also be fused or unfused with one or more cyclic hydrocarbon, heteroaryl, or heterocyclic rings.
"Substituted aryl" is aryl having one or more non-interfering groups as substituents. For substitutions on a phenyl ring, the substituents may be in any orientation (i.e., ortho, meta or para).
"Heteroaryl" is an aryl group containing from one to four N, 0, or S atoms(s) or a combination thereof, which heteroaryl group is optionally substituted at carbon or nitrogen atom(s) with C 1-6 alkyl, -CF3, phenyl, benzyl, or thienyl, or a carbon atom in the heteroaryl group together with an oxygen atom form a carbonyl group, or which heteroaryl group is optionally fused with a phenyl ring. Heteroaryl rings may also be fused with one or more cyclic hydrocarbon, heterocyclic, aryl, or heteroaryl rings.
Heteroaryl includes, but is not limited to, 5-membered heteroaryls having one hetero atom (e.g., thiophenes, pyrroles, furans); 5 membered heteroaryls having two heteroatoms in 1,2 or 1,3 positions (e.g., oxazoles, pyrazoles, imidazoles, thiazoles, purines); 5-membered heteroaryls having three heteroatoms (e.g., triazoles, thiadiazoles); 5-membered heteroaryls having 3 heteroatoms; 6-membered heteroaryls with one heteroatom (e.g., pyridine, quinoline, isoquinoline, phenanthrine, 5,6-cycloheptenopyridine); 6-membered heteroaryls with two heteroatoms (e.g., pyridazines, cinnolines, phthalazines, pyrazines, pyrimidines, quinazolines);
membered heretoaryls with three heteroatoms (e.g., 1,3,5-triazine); and 6-membered heteroaryls with four heteroatoms.
"Substituted heteroaryl" is heteroaryl having one or more non--interfering groups as substituents.
"Heterocycle" or "heterocyclic" means one or more rings of 5, 6 or 7 atoms with or without unsaturation or aromatic character and at least one ring atom which is not carbon. Preferred heteroatoms include sulfur, oxygen, and nitrogen.
Multiple rings may be fused, as in quinoline or benzofuran.
"Substituted heterocycle" is heterocycle having one or more side chains formed from non-interfering substituents.
"Non-interfering substituents" are those groups that yield stable compounds.
Suitable non-interfering substituents or radicals include, but are not limited to, halo, C l-C 10 alkyl, C2-C 10 alkenyl, C2-C 10 alkynyl, C 1-C 10 alkoxy, C7-C12 aralkyl, C7-C 12 alkaryl, C3-C 10 cycloalkyl, C3-C 10 cycloalkenyl, phenyl, substituted phenyl, toluoyl, xylenyl, biphenyl, C2-C 12 alkoxyalkyl, C7-C 12 alkoxyaryl, C7-C 12 aryloxyalkyl, C6-C 12 oxyaryl, C 1-C6 alkylsulfinyl, C 1-C 10 alkylsulfonyl, -(CH2)m-O-(C 1-C 10 alkyl) wherein m is from I to 8, aryl, substituted aryl, substituted alkoxy, fluoroalkyl, heterocyclic radical, substituted heterocyclic radical, nitroalkyl, -NO2, -CN, -NRC(O)-(C 1-C 10 alkyl), -C(O)-(C 1-C 10 alkyl), C2-C 10 thioalkyl, -C(O)O-(C 1-C 10 alkyl), -OH, -SO2, =S, -COOH, -NR, carbonyl, -C(O)-(C 1-C 10 alkyl)-CF3, -C(O)-CF3, -C(O)NR2, -(C 1-C 10 alkyl)-S-(C6-C 12 aryl), -C(O)-(C6-C 12 aryl), -(CH2)m-O-(CH2)m-O-(C1-C10 alkyl) wherein each m is from 1 to 8, -C(O)NR, -C(S)NR, -SO2NR, -NRC(O)NR, -NRC(S)NR, salts thereof, and the like. Each R as used herein is H, alkyl or substituted alkyl, aryl or substituted aryl, aralkyl, or alkaryl.
The term "drug", "biologically active molecule", "biologically active moiety"
or "biologically active agent", when used herein means any substance which can affect any physical or biochemical properties of a biological organism, including but not limited to viruses, bacteria, fungi, plants, animals, and humans. In particular, as used herein, biologically active molecules include any substance intended for diagnosis, cure mitigation, treatment, or prevention of disease in humans or other animals, or to otherwise enhance physical or mental well-being of humans or animals.
Examples of biologically active molecules include, but are not limited to, peptides, proteins, enzymes, small molecule drugs, dyes, lipids, nucleosides, oligonucleotides, cells, viruses, liposomes, microparticles and micelles. Classes of biologically active agents that are suitable for use with the invention include, but are not limited to, antibiotics, fungicides, anti-viral agents, anti-inflammatory agents, anti-tumor agents, cardiovascular agents, anti-anxiety agents, hormones, growth factors, steroidal agents, and the like.
"Hydrophobic" refers to molecules having a greater solubility in octanol than in water, typically having a much greater solubility in octanol. Conversely, "hydrophilic" refers to molecules having a greater solubility in water than in octanol.
"Poly(hydroxyester)" refers to polymers comprising repeating monomer units of -O-R-C(O)-, wherein R is alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocycle, or substituted heterocycle. Exemplary poly(hydroxyesters) include poly(lactide), poly(glycolide), poly(lactide/glycolide) copolymer, poly(butyrolactide), and polycaprolactone.
"Oligomer" refers to short monomer chains comprising 2 to about 10 monomer units.

II. The Multi-Arm Block Copolymer In one aspect, the present invention provides a multi-arm block copolymer having a hydrophobic core region defined by a central core molecule and hydrophobic polymer arms covalently attached to the central core molecule and an outer hydrophilic region defined by a hydrophilic polymer covalently attached to the hydrophobic polymer arms. Each arm of the multi-arm structure comprises an inner (i.e. closer to the central core molecule) hydrophobic polymer segment and an outer (i.e. further from the central core molecule) hydrophilic polymer segment.
In aqueous solution, it is believed that the multi-arm block copolymer acts as a unimolecular micelle having a central hydrophobic core region bounded by a hydrophilic region. As demonstrated in the experimental section, the multi-arm block copolymers of the invention are capable of increasing the aqueous solubility of hydrophobic biologically active agents or drugs by encapsulating or physically entrapping the hydrophobic drug molecule within the hydrophobic core region of the multi-arm block copolymer structure. Thus, the multi-arm block copolymers are useful as drug delivery vehicles, particularly for hydrophobic drug molecules.
"Encapsulation" or "entrapment" is intended to refer to physical confinement of the drug molecule within the hydrophobic region of the copolymer, rather than covalent attachment to the copolymer.
Compared to conventional linear micelle structures, the unimolecular nature of the multi-arm block copolymers of the invention results in less sensitivity to concentration, such that the block copolymers of the invention are less likely to release the entrapped drug molecules at an undesirably rapid rate. The multi-arm block copolymers of the invention are covalently bound molecular units rather than molecular aggregates and, thus, are substantially precluded from disassembly in circulation in the absence of hydrolytically unstable linkages within the polymer segments specifically intended to degrade the copolymer. Further, since chemical modification of the drug molecules is not required to obtain an increase in solubility, the possibility of the copolymer reducing efficacy of the entrapped drug is greatly reduced.
Although not bound by any particular theory, it is believed that the level of hydrophobicity and size of the hydrophobic polymer affect the drug loading and drug release characteristics of the multi-arm block copolymer. In general, it is believed that larger hydrophobic polymer segments and hydrophobic polymer segments formed from polymers having relatively greater degrees of hydrophobicity will result in higher drug loading and slower drug release profiles in solution.
Conversely, smaller hydrophobic polymer segments and hydrophobic polymer segments formed from polymers having relatively lower degrees of hydrophobicity will result in reduced drug loading and more rapid drug release.
Further, without being bound by theory, it is believed that the number of arms of the multi-arm block polymer also impacts the drug loading and drug release characteristics of the copolymer. Generally, the presence of fewer copolymer arms results in reduced drug loading. However, the use of a copolymer with a very large number of arms can also reduce drug loading because of the substantial increase in density and concomitant reduction in interstitial space within the hydrophobic core region of the copolymer structure. Generally, the presence of fewer copolymer arms will also result in more rapid drug release. This is attributed, at least in part, to the effect of aggregation of multi-arm block copolymers and entrapment of drug molecules within a hydrophobic region defined by the aggregated copolymers.
Aggregation of the multi-arm block copolymers creates hydrophobic regions that are not unimolecular in nature. Instead, a multi-arm block copolymer aggregate behaves in a manner analogous to conventional linear micelles. Reductions in concentration can break up the copolymer aggregate and release a portion of the drug molecules entrapped within the hydrophobic region created by the aggregation. Copolymers with a higher number of arms are less susceptible to the aggregation effect and less likely to have drug release characteristics that depend on concentration. In light of the foregoing, an optimal range for the number of arms of the block copolymer can be determined such that both desirable drug loading and drug release characteristics are obtained for any particular hydrophobic drug. In most embodiments, the number of arms is in the range of 3 to about 25, preferably at least 5, more preferably at least about 8, and most preferably at least about 10.
The hydrophobic and hydrophilic polymer segments are preferably not "hyper-branched" or dendritic in nature, such as the dendrimers described in U.S.
Patent No. 5,830,986, wherein branched compounds are attached in numerous successive layers to a central core. Instead, both polymer segments are preferably substantially linear in nature as depicted in Figure 1. However, some branching in either polymer segment may be present. For example, a branched poly(ethylene glycol) polymer comprising two polymer backbones attached to lysine linker is used as the hydrophilic polymer in several appended examples.
Although the specific examples of multi-arm block copolymers in the appended experimental section utilize the same block copolymer structure for each copolymer arm, it is possible to utilize different copolymer structures within the same multi-arm structure. In other words, the present invention includes embodiments wherein more than one particular hydrophobic/hydrophilic polymer combination is attached to the same core molecule.
A. The Central Core The central core molecule is derived from a molecule that provides a number of polymer attachment sites equal to the number of desired copolymer arms.
Preferably, the central core molecule of the multi-arm block copolymer structure is the residue of a polyol having at least three hydroxyl groups available for polymer attachment. A "polyol" is a molecule comprising a plurality of available hydroxyl groups. Depending on the desired number of copolymer arms, the polyol will typically comprise 3 to about 25 hydroxyl groups, preferably at least 5, more preferably at least about 8, and most preferably at least about 10. The polyol may include other protected or unprotected functional groups as well without departing from the invention. Although the spacing between hydroxyl groups will vary from polyol to polyol, there are typically I to about 20 atoms, such as carbon atoms, between each hydroxyl group, preferably 1 to about 5. As would be understood in the art, by "residue" is meant the portion of the polyol molecule remaining after attachment of the copolymer arms. Preferred polyols include glycerol, reducing sugars such as sorbitol, pentaerythritol, and glycerol oligomers, such as hexaglycerol.
As noted in the appended examples, a 21-arm block copolymer can be synthesized using hydroxypropyl-(3-cyclodextrin, which has 21 available hydroxyl groups.
The particular polyol chosen will depend on the desired number of hydroxyl groups needed for attachment to the copolymer arms.
B. The Hydrophobic Polymer The particular hydrophobic polymer used in the present invention will depend, at least in part, on the desired drug loading and drug release characteristics, since as explained above, the size and hydrophobicity of the hydrophobic polymer segment will affect those characteristics. The hydrophobic polymer should be generally non-toxic and biocompatible, meaning that the polymer is capable of coexistence with living tissues or organisms without causing harm. In preferred embodiments, the hydrophobic polymer segments comprises a poly(hydroxyester), a poly(alkylene oxide) other than poly(ethylene glycol), such as poly(propylene oxide) (PPO) or poly(butylene oxide) (PBO), or copolymers thereof. Exemplary poly(hydroxyester) polymers include poly(lactide), poly(glycolide), poly(lactide/glycolide) copolymer, poly(butyrolactide) and polycaprolactone. The hydrophobic polymer segment of the block copolymer will typically have a number average molecular weight of about Da to about 100,000 Da, preferably about 10,000 Da to about 40,000 Da. For example, hydrophobic polymer segments having a molecular weight of about 5,000 Da, about 10,000 Da, about 15,000 Da, about 20,000 Da, about 25,000 Da or about 30,000 Da are useful in the present invention.
In addition to being hydrophobic, the poly(hydroxyester) polymers also include one or more hydrolytically or enzymatically degradable linkages, such as ester linkages. Typically, use of these polymers results in the formation of degradable linkages between the central core molecule and the polymer segment, within the polymer segment, between the hydrophobic polymer segment and the hydrophilic polymer segment, or some combination thereof. As used herein, the hydrophobic polymer is said to comprise a degradable linkage if a linkage is located at any of the above-listed locations. The use of a hydrophobic polymer with one or more degradable linkages allows the multi-arm block copolymer to degrade in solution over time, thus increasing renal clearance of the copolymer. In addition, the degradable linkages provide an additional feature of these polymers, i.e., the ability to control the rate of release of the entrapped drug.

C. The Hydrophilic Polymer The hydrophilic polymer segment may comprise any hydrophilic polymer. As with the hydrophobic polymer, the hydrophilic polymer should be generally non-toxic and biocompatible, meaning that the polymer is capable of coexistence with living tissues or organisms without causing harm. Preferably, poly(ethylene glycol) (PEG) is used as the hydrophilic polymer segment. The term PEG includes poly(ethylene glycol) in any of its linear, branched or multi-arm forms, including alkoxy PEG, bifunctional PEG, forked PEG, branched PEG, pendant PEG, or PEG with degradable linkages therein, to be more fully described below.
In its simplest form, PEG has the formula -CH2CH2O-(CH2CH2O)õ-CH2CH2-, where n is from about 10 to about 4000, typically from about 20 to about 500.
PEGs having a number average molecular weight of from about 500 Da to about 100,000 Da, preferably about 1,000 Da to about 20,000 Da are particularly useful as the hydrophilic polymer segment. For example, PEG polymer segments having a molecular weight of about 1,000 Da, about 5,000 Da, about 10,000 Da, about 15,000 Da, or about 20,000 Da are useful in the present invention.
In one form useful in the present invention, free or non-bound PEG is a linear polymer terminated at each end with hydroxyl groups:
HO-CH2CH2O-(CH2CH2O),-CH2CH2-OH
The above polymer, alpha-,omega-dihydroxylpoly(ethylene glycol), can be represented in brief form as HO-PEG-OH where it is understood that the -PEG-symbol represents the following structural unit:
-CH2CH2O-(CH2CH2O)õ-CH2CH2-where n typically ranges from about 10 to about 4000.
Another type of PEG useful in forming the conjugates of the invention is methoxy-PEG-OH, or mPEG in brief, in which one terminus is the relatively inert methoxy group, while the other terminus is a hydroxyl group that is subject to ready chemical modification. The structure of mPEG is given below.
CH3O-(CHZCH2O)õ-CHZCH2-OH
where n is as described above. The use of hydrophilic polymer segments in the form of mPEG is exemplified in Examples 1 and 4.
Multi-armed or branched PEG molecules, such as those described in U.S.
Patent No. 5,932,462 can also be used as thehydrophilic PEG polymer segment. For example, the hydrophilic PEG segment can have the structure:

Polya P
R"-C-polye Q
Formula I
wherein:
polya and polyb are PEG backbones, such as methoxy poly(ethylene glycol);
R" is a nonreactive moiety, such as H, methyl or a PEG backbone; and P and Q are nonreactive linkages. In a preferred embodiment, the branched polymer segment comprises methoxy poly(ethylene glycol) disubstituted lysine.
Use of such a branched PEG structure is exemplified in Examples 2, 5, and 7.
The PEG polymer may alternatively coinprise a forked PEG. An example of a forked PEG is represented by PEG-YCHZ2, where Y is a linking group and Z is an activated terminal group linked to CH by a chain of atoms of defined length.
International Application No. PCT/US99/05333 discloses various forked PEG structures capable of use in the present invention. The chain of atoms linking the Z functional groups to the branching carbon atom serve as a tethering group and may comprise, for example, alkyl chains, ether chains, ester chains, amide chains and combinations thereof.
The PEG polymer may comprise a pendant PEG molecule having reactive groups, such as carboxyl, covalently attached along the length of the PEG
segment rather than at the end of the PEG chain. The pendant reactive groups can be attached to the PEG segment directly or through a linking moiety, such as alkylene.
In addition to the above-described forms of PEG, the polymer can also be prepared with one or more weak or degradable linkages in the segment, including any of the above described polymers. For example, PEG can be prepared with ester linkages in the polymer segment that are subject to hydrolysis. As shown below, this hydrolysis results in cleavage of the polymer into fragments of lower molecular weight:
-PEG-COz-PEG- + H20 --~ -PEG-CO2H + HO-PEG-Similarly, the PEG polymer can be covalently attached to the hydrophobic polymer segment or other molecules through a weak or degradable linkage moiety.
Other hydrolytically degradable linkages, useful as either a degradable linkage within a polymer segment or as a degradable linkage connecting the PEG polymer to other molecules include carbonate linkages; imine linkages resulting, for example, from reaction of an amine and an aldehyde (see, e.g_, Ouchi et al., Polymer Preprints, 38(1):582-3 (1997)); phosphate ester linkages formed, for example, by reacting an alcohol with a phosphate group;
hydrazone linkages which are typically formed by reaction of a hydrazide and an aldehyde; acetal linkages that are typically formed by reaction between an aldehyde and an alcohol; orthoester linkages that are, for example, formed by reaction between a formate and an alcohol; peptide linkages formed by an amine group, e.g., at an end of a polymer such as PEG, and a carboxyl group of a peptide; and oligonucleotide linkages formed by, for example, a phosphoramidite group, e.g., at the end of a polymer, and a 5' hydroxyl group of an oligonucleotide_ l2 It is understood by those skilled in the art that the term poly(ethylene glycol) or PEG represents or includes all the above forms of PEG.
In some embodiments, it may be desirable to covalently attach a targeting moiety or drug molecule to the hydrophilic polymer segment. As used herein, "targeting moiety" includes any chemical moiety capable of binding to, or otherwise exhibiting an affinity for, a particular type of tissue or component thereof.
The addition of a targeting moiety to the copolymer structure can direct the copolymer to particular sites within the body for targeted release of the physically entrapped drug.
For example, certain moieties are known to exhibit an affinity for hydroxyapatite surfaces (i.e. calcium phosphate), such as bone. Exemplary hydroxyapatite-targeting moieties include tetracycline, calcein, bisphosphonates, such as 4-amino-l-hydroxybutane-1,1-diphosphonic acid, ditetrabutylammonium salt (AHBDP) or derivatives thereof, polyaspartic acid, polyglutamic acid, and aminophosphosugars.
Additional targeting moieties include proteins, antibodies, antibody fragments, peptides, carbohydrates, lipids, oligonucleotides, DNA, RNA, or small molecules having a molecular weight less than 2000 Daltons.
The PEG polymer segment may further include one or more capping groups covalently attached to the PEG molecule, such as at a terminus of the PEG
segment distal from the point of attachment to the hydrophobic polymer. The capping group can be a relatively inert group, such as an alkoxy group (e.g. methoxy or ethoxy).
Alternatively, the capping group can be a reactive functional group, such as a functional group capable of reacting with a targeting moiety or drug molecule so that such molecules can be attached to the PEG polymer as described above.
Exemplary functional groups, optionally in protected form, include hydroxyl, protected hydroxyl, active ester (e.g. N-hydroxysuccinimidyl, 1-benzotriazolyl, p-nitrophenyl, or imidazolyl esters), active carbonate (e.g. N-hydroxysuccinimidyl, 1-benzotriazolyl, p-nitrophenyl, or imidazolyl carbonate), acetal, aldehyde, aldehyde hydrates, alkyl or aryl sulfonate, halide, disulfide derivatives such as o-pyridyl disulfidyl, alkenyl, acrylate, methacrylate, acrylamide, active sulfone, amine, protected amine, hydrazide, protected hydrazide, thiol, protected thiol, carboxylic acid, protected carboxylic acid, isocyanate, isothiocyanate, maleimide, vinylsulfone, dithiopyridine, vinylpyridine, iodoacetamide, epoxide, glyoxals, diones, mesylates, tosylates, or tresylate.

As would be understood in the art, the tern, protected" refers to the presence of a protecting group or moiety that prevents reaction of the chemically reactive functional group under certain reaction conditions. The protecting group will vary depending on the type of chemically reactive group being protected and the reaction conditions employed. For example, if the chemically reactive group is an amine or a hydrazide, the protecting group can be selected from the group of tert-butyloxycarbonyl (t-Boc) and 9-fluorenylmethoxycarbonyl (Fmoc). If the chemically reactive group is a thiol, the protecting group can be orthopyridyldisulfide.
If the chemically reactive group is a carboxylic acid, such as butanoic or propionic acid, or a hydroxyl group, the protecting group can be benzyl or an alkyl group such as methyl, ethyl, or tert-butyl. Other protecting groups known in the art may also be used in the invention, see for example, Greene, T.W., et al., PROTECTIVE GROUPS IN ORGANIC
SYNTHESIS, 2nd ed., John Wiley & Sons, New York, NY (1991).
Specific examples of functional groups for the hydrophilic polymer include N-succinimidyl carbonate (see e.g., U.S. Patent Nos. 5,281,698, 5,468,478), amine (see, e.g., Buckmann et al. Makromol.Chem. 182:1379 (1981), Zaplipsky et al. Eur.
Polym.
J. 19:1177 (1983)), hydrazide (See, e.g., Andresz et al. Makromol. Chem.
179:301 (1978)), succinimidyl propionate and succinimidyl butanoate (see, e.g., Olson et al. in Poly(ethylene glycol) Chemistry & Biological Applications, pp 170-181, Harris &
Zaplipsky Eds., ACS, Washington, DC, 1997; see also U.S. Patent No.
5,672,662), succinimidyl succinate (See, e.g., Abuchowski et al. Cancer Biochem. Biophys.
7:175 (1984) and Joppich et al. Macrolol. Chem. 180:1381 (1979), succinimidyl ester (see, e.g., U.S. Patent No. 4,670,417), benzotriazole carbonate (see, e.g., U.S.
Patent No.
5,650,234), glycidyl ether (see, e.g., Pitha et al. Eur. J. Biochem. 94:11 (1979), Elling et al., Biotech. Appl. Biochem. 13:354 (1991), oxycarbonylimidazole (see, e.g., Beauchamp, et al., Anal. Biochem. 131:25 (1983), Tondelli et al. J. Controlled Release 1:251 (1985)), p-nitrophenyl carbonate (see, e.g., Veronese, et al., Appl.
Biochem. Biotech., 11:141 (1985); and Sartore et al., Appl. Biochem. Biotech., 27:45 (1991)), aldehyde (see, e.g., Harris et al. J. Polym. Sci. Chem. Ed. 22:341 (1984), U.S.
Patent No. 5,824,784, U.S. Patent 5,252,714), maleimide (see, e.g., Goodson et al.
Bio/Technology 8:343 (1990), Romani et al. in Chemistry of Peptides and Proteins 2:29 (1984)), and Kogan, Synthetic Comm. 22:2417 (1992)), orthopyridyl-disulfide (see, e.g., Woghiren, et al. Bioconj. Chem. 4:314 (1993)), acrylol (see, e.g., Sawhney WO 03/000777 PCTlUS02l21872 et at., Macromolecules. 26:581 (1993)), vinylsulfone (see, e.g., U.S. Patent No.
5,900,461).

D. Exemplary Multi-Arm Block Copolymer Structures More specific structural embodiments of the block copolymers of the invention will now be described. The specific structures shown below are presented as exemplary structures only, and are not intended to limit the scope of the invention.
In one embodiment, a block copolymer of the invention is represented by Formula II:
A(-O-B-O-C-D), wherein:
A is a central core molecule as described above, such as a residue of a polyol having at least three hydroxyl groups, 0 is oxygen, B is a hydrophobic polymer segment as described above, C is a hydrophilic polymer segment as described above, D is a capping group as described above, and n is 3 to about 25, preferably at least about 5, more preferably at least about 8, and most preferably at least about 10.
In a further embodiment, the block copolymer has the following structure represented by Formula III:
(E-C-0-B-O-)PA(-O-B-0-C-D)m wherein:
A, 0, B, C are as described above, D is an alkoxy or hydroxy group, p is at least 1, the sum of m and p is from 3 to about 25, and E is a functional group as described above.
In a third embodiment, the copolymer has the following structure represented by Formula IV:
(T-C-0-B-O-)PA(-O-B-0-C-D)m wherein:
A, 0, B, C are as described above, D is a capping group, p is at least 1, the sum of m and p is from 3 to about 25, and T is a targeting moiety or drug moiety as described above, such as a bisphosphonate.
Regarding Formulas III and IV above, in one embodiment, p is 1 to about 5, preferably I to about 3, and the sum of m and p is about 6 to about 21, preferably about 8 to about 15.
Formula V below is an exemplary 8-arm PPO-PEG block copolymer made in accordance with the invention:

/ PEG OH HO PEGO
I I
PPO PPO
O O O O~
/O \ 4 O
PPO
\ / PPO / PO

HO PEG/ \ PEC-OH \ PEC-OH

Formula V
Formula VI below is an exemplary 8-arm degradable poly(lactide)-poly(ethylene glycol) (PLA-PEG) block copolymer of the invention:

PLA IN, O / PLA
O O O

/O \ 4 O
PLA
I PLA LA
H3CO-PEG \
PEG OCH3 pEG-OCH3 Formula VI

E. The Hydrophobic Drug The hydrophobic biologically active moiety or drug may be any biologically active hydrophobic compound that would benefit from increased aqueous solubility.
The entrapped or encapsulated drug may be utilized per se or in the form of a pharmaceutically acceptable salt. If used, a salt of the drug compound should be both pharmacologically and pharmaceutically acceptable, but non-pharmaceutically acceptable salts may conveniently be used to prepare the free active compound or pharmaceutically acceptable salts thereof and are not excluded from the scope of this invention. Such pharmacologically and pharmaceutically acceptable salts can be prepared by reaction of the drug with an organic or inorganic acid, using standard methods detailed in the literature. Examples of useful salts include, but are not limited to, those prepared from the following acids: hydrochloric, hydrobromic, sulfuric, nitric, phosphoric, maleic, acetic, salicyclic, p-toluenesulfonic, tartaric, citric, methanesulphonic, formic, malonic, succinic, naphthalene-2-sulphonic and benzenesulphonic, and the like. Also, pharmaceutically acceptable salts can be prepared as alkaline metal or alkaline earth salts, such as sodium, potassium, or calcium salts of a carboxylic acid group.
Examples of hydrophobic drug molecules that may be encapsulated within the multi-arm block copolymers of the invention include, but are not limited to, abietic acid, aceglatone, acenaphthene, acenocournarol, acetohexamide, acetomeroctol, acetoxolone, acetyldigitoxins, acetylene dibromide, acetylene dichloride, acetylsalicylic acid, alantolactone, aldrin, alexitol sodium, allethrin, allylestrenol, allylsulfide, alprazolam, aluminum bis(acetylsalicylate), ambucetamide, aminochlothenoxazin, aminoglutethimide, amyl. chloride, androstenediol, anethole trithone, anilazine, anthralin, Antimycin A, aplasmomycin, arsenoacetic acid, asiaticoside, asternizole, aurodox, aurothioglycanide, 8-azaguanine, azobenzene, baicalein, Balsam Peru, Balsam Tolu, barban, baxtrobin, bendazac, bendazol, bendroflumethiazide, benomyl, benzathine, benzestrol, benzodepa, benzoxiquinone, benzphetamine, benzthiazide, benzyl benzoate, benzyl cinnamate, bibrocathol, bifenox, binapacryl, bioresmethrin, bisabolol, bisacodyl, bis(chlorophenoxy)methane, bismuth iodosubgallate, bismuth subgallate, bismuth tannate, Bisphenol A, bithionol, bornyl, bromoisovalerate, bornyl chloride, bomyl isovalerate, bornyl salicylate, brodifacoum, bromethalin, broxyquinoline, bufexamac, butamirate, butethal, buthiobate, butylated hydroxyanisole, butylated hydroxytoluene, calcium iodostearate, calcium saccharate, calcium stearate, capobenic acid, captan, carbamazepine, carbocloral, carbophenothin, carboquone, carotene, carvacrol, cephaeline, cephalin, chaulmoogric acid, chenodiol, chitin, chlordane, chlorfenac, chlorfenethol, chlorothalonil, chlorotrianisene, chlorprothixene, chlorquinaldol, chromonar, cilostazol, cinchonidine, citral, clinofibrate, clofaziminc, clofibrate, cloflucarban, clonitrate, clopidol, clorindione, cloxazolam, coroxon, corticosterone, cournachlor, coumaphos, coumithoate cresyl acetate, crimidine, crufomate, cuprobam, cyamemazine, cyclandelate, cyclarbamate cymarin, cyclosporin A, cypermethril, dapsone, defosfamide, deltamethrin, deoxycorticocosterone acetate, desoximetasone, dextromoramide, diacetazoto, dialifor, diathymosulfone, decapthon, dichlofluani, dichlorophen, dichlorphenamide, dicofol, dicryl, dicumarol, dienestrol, diethylstilbestrol, difenamizole, dihydrocodeinone enol acetate, dihydroergotamine, dihydromorphine, dihydrotachysterol, dimestrol, dimethisterone, dioxathion, diphenane, N-(1,2-diphenylethyl)nicotinamide, 3,4-di-[1-methyl 6-nitro-3-indolyl]-1H-pyrrole-2,5-dione (MNIPD), dipyrocetyl, disulfamide, dithianone, doxenitoin, drazoxolon, durapatite, edifenphos, emodin, enfenamic acid, erbon, ergocorninine, erythrityl tetranitrate, erythromycin stearate, estriol, ethaverine, ethisterone, ethyl biscournacetate, ethylhydrocupreine, ethyl menthane carboxamide, eugenol, euprocin, exalamide, febarbamate, fenalamide, fenbendazole, fenipentol, fenitrothion, fenofibrate, fenquizone, fenthion, feprazone, flilpin, filixic acid, floctafenine, fluanisone, flumequine, fluocortin butyl, fluoxymesterone, flurothyl, flutazolam, fumagillin, 5-furftiryl-5-isopropylbarbituric acid, fusaftmgine; glafenine, glucagon, glutethimide, glybuthiazole, griseofulvin, guaiacol carbonate, guaiacol phosphate;
halcinonide, hematoporphyrin, hexachlorophene, hexestrol, hexetidine, hexobarbital, hydrochlorothiazide, hydrocodone, ibuproxam, idebenone, indomethacin, inositol niacinate, iobenzamic acid, iocetamic acid, iodipamide, iomeglamic acid, ipodate, isometheptene, isonoxin, 2-isovalerylindane-1,3-dione, josamycin, 11-ketoprogesterone, laurocapram, 3-0-lauroylpyridoxol diacetate, lidocaine, lindane, linolenic acid, liothyronine, lucensomycin, mancozeb, mandelic acid, isoamyl ester, mazindol, mebendazole, mebhydroline, mebiquine, melarsoprol, melphalan, menadione, menthyl valerate, mephenoxalone, mephentermine, mephenytoin, meprylcaine, mestanolone, mestranol, mesulfen, metergoline, methallatal, methandriol, methaqualone, methylcholanthrene, methylphenidate, 17-methyltestosterone, metipranolol, minaprine, myoral, naftalofos, naftopidil, naphthalene, 2-naphthyl lactate, 2-(2-naphthyloxy)ethanol, naphthyl salicylate, naproxen, nealbarbital, nemadectin, niclosamide, nicoclonate, nicomorphine, nifuroquine, nifuroxazide, nitracrine, nitromersol, nogalamycin, nordazepam, norethandrolone, norgestrienone, octaverine, oleandrin, oleic acid, oxazeparn, oxazolam, oxeladin, oxwthazaine, oxycodone, oxymesterone, oxyphenistan acetate, paclitaxel, paraherquamide, parathion, pemoline, pentaerythritol tetranitrate, pentylphenol, perphenazine, phencarbamide, pheniramine, 2-phenyl-6-chlorophenol, phenthnethylbarbituric acid, phenytoin, phosalone, O-phthalylsulfathiazole, phylloquinone, picadex, pifarnine, piketopfen, piprozolin, pirozadil, pivaloyloxymethyl butyrate, plafibride, plaunotol, polaprezinc, polythiazide, probenecid, progesterone, promegestone, propanidid, propargite, propham, proquazone, protionamide, pyrimethamine, pyrimithate, pyrvinium pamoate, quercetin, quinbolone, quizalofo-ethyl, rafoxanide, rescinnamine, rociverine, ronnel, salen, scarlet red, siccanin, simazine, simetride, simvastatin, sobuzoxane, solan, spironolactone, squalene, stanolone, sucralfate, sulfabenz, sulfaguanole, sulfasalazine, sulfoxide, sulpiride, suxibuzone, talbutal, terguide, testosterone, tetrabromocresol, tetrandrine, thiacetazone, thiocolchicine, thioctic acid, thioquinox, thioridazine, thiram, thymyl N-isoamylcarbamate, tioxidazole, tioxolone, tocopherol, tolciclate, tolnaftate, triclosan, triflusal, triparanol, ursolic acid, valinomycin, verapamil, vinblastine, vitamin A, vitamin D, vitamin E, xenbucin, xylazine, zaltoprofen, and zearalenone.

III. Pharmaceutical Compositions Comprising the Multi-Arm Block Copolymer In another aspect, the invention provides pharmaceutical formulations or compositions, both for veterinary and for human medical use, comprising a multi-arm block copolymer as described above and at least one biologically active agent entrapped within the hydrophobic core region of the multi-arm block copolymer.
As noted previously, incorporation of a hydrophobic drug into the block copolymer structure of the invention increases the aqueous solubility of the drug, which can enhance the circulating residence time of the drug upon administration to a mammal.
The pharmaceutical formulation may include one or more pharmaceutically acceptable carriers, and optionally any other therapeutic ingredients, stabilizers, or the like. The carrier(s) must be pharmaceutically acceptable in the sense of being compatible with the other ingredients of the formulation and not unduly deleterious to the recipient thereof. The compositions of the invention may also include polymeric excipients/additives or carriers, e.g., polyvinylpyrrolidones, derivatized celluloses such as hydroxymethylcellulose, hydroxyethylcellulose, and hydroxypropylmethylcellulose, Ficolls (a polymeric sugar), hydroxyethylstarch (HES), dextrates (e.g., cyclodextrins, such as 2-hydroxypropyl-(3-cyclodextrin and sulfobutylether-(3-cyclodextrin), polyethylene glycols, and pectin. The compositions may further include diluents, buffers, binders, disintegrants, thickeners, lubricants, preservatives (including antioxidants), flavoring agents, taste-masking agents, inorganic salts (e.g., sodium chloride), antimicrobial agents (e.g., benzalkonium chloride), sweeteners, antistatic agents, surfactants (e.g., polysorbates such as "TWEEN 20" and "TWEEN 80", and pluronics such as F68 and F88, available from BASF), sorbitan esters, lipids (e.g., phospholipids such as lecithin and other phosphatidylcholines, phosphatidylethanolamines, fatty acids and fatty esters, steroids (e.g., cholesterol)), and chelating agents (e.g., EDTA, zinc and other such suitable cations). Other pharmaceutical excipients and/or additives suitable for use in the compositions according to the invention are listed in "Remington: The Science &
Practice of Pharmacy", 19`h ed., Williams & Williams, (1995), and in the "Physician's Desk Reference", 52"d ed., Medical Economics, Montvale, NJ (1998), and in "Handbook of Pharmaceutical Excipients", Third Ed., Ed. A.H. Kibbe, Pharmaceutical Press, 2000.
The block copolymers of the invention may be formulated in compositions including those suitable for oral, buccal, rectal, topical, nasal, ophthalmic, or parenteral (including intraperitoneal, intravenous, subcutaneous, or intramuscular injection) administration. The block copolymers may also be used in formulations suitable for inhalation. The compositions may conveniently be presented in unit dosage form and may be prepared by any of the methods well known in the art of pharmacy. All methods include the step of bringing the block copolymer with drug entrapped therein into association with a carrier that constitutes one or more accessory ingredients. In general, the compositions are prepared by bringing the block copolymer/drug formulation into association with a liquid carrier to form a solution or a suspension, or alternatively, bringing the block copolymer/drug formulation into association with formulation components suitable for forming a solid, optionally a particulate product, and then, if warranted, shaping the product into a desired delivery form. Solid formulations of the invention, when particulate, will typically comprise particles with sizes ranging from about 1 nanometer to about 500 microns. In general, for solid formulations intended for intravenous administration, particles will typically range from about 1 nm to about 10 microns in diameter.
The amount of the biologically active agent or drug in the formulation will vary depending upon the specific drug employed, its molecular weight, and other factors such as dosage form, target patient population, and other considerations, and will generally be readily determined by one skilled in the art. The amount of biologically active agent in the copolymer formulation will be that amount necessary to deliver a therapeutically effective amount of the drug to a patient in need thereof to achieve at least one of the therapeutic effects associated with the drug. In practice, this will vary widely depending upon the particular drug, its activity, the severity of the condition to be treated, the patient population, the stability of the formulation, and the like. Compositions will generally contain anywhere from about 1% by weight to about 30% by weight drug, typically from about 2% to about 20% by weight drug, and more typically from about 3% to about 15% by weight drug, and will also depend upon the relative amounts of excipients/additives contained in the composition. More specifically, the composition will typically contain at least about one of the following percentages of the entrapped drug: 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 12%, 14%, 16%, 18%, 20%, or more by weight.

IV. Methods of Making the Block Copolymer The multi-arm block copolymers of the invention can be prepared by simply covalently attaching a preformed hydrophobic polymer segment to the core molecule followed by covalently attaching a preformed hydrophilic polymer segment to the hydrophobic polymer segment. Alternatively, one or more of the polymer segments can be prepared by directly polymerizing monomer units of the polymer using, for example, a ring-opening polymerization technique.
For example, in order to synthesize a poly(propylene oxide)-poly(ethylene glycol) copolymer (PPO-PEG) on a polyol core, the propylene oxide monomers can be directly polymerized onto the polyol core by base-initiated ring-opening polymerization in a suitable solvent. Suitable bases include potassium naphthalenide, sodium hydride, sodium or potassium alkoxides, or other strong bases. Suitable solvents include tetrahydrofuran, dioxane, or toluene. In a second step, the product of the first reaction is reacted with monomer units of ethylene oxide using a base and solvent as described for the first reaction. The molecular weight of the PPO
polymer formed in the first step is controlled by the molar ratio of the propylene oxide to that of the polyol. The molecular weight of the PEG polymer formed in the second step is controlled by the molar ratio of the ethylene oxide to that of the PPO polymer formed in the first step.
In those embodiments utilizing a poly(hydroxyester) hydrophobic polymer segment and a PEG hydrophilic polymer segment, it is preferable to directly polymerize the hydroxyester monomer onto the core molecule (e.g. a polyol) to create the poly(hydroxyester) portion of the copolymer, followed by covalent attachment of the PEG polymer to the distal terminus of the poly(hydroxyester) segment.

V. Methods of Loading the Drug into the Multi-Arm Block Copolymer There are several methods for entrapping a biologically active agent or drug within the hydrophobic region of the block copolymers of the invention. In a first method, the hydrophobic drug and the copolymer are co-dissolved in an organic solvent and then dried to form a solid product. The solid product is redissolved in aqueous solution and filtered to remove insoluble particles prior to use. In a second method, the hydrophobic drug is suspended in an aqueous solution of the copolymer and subjected to ultrasonication for several hours in order to intimately contact the drug molecules and the hydrophobic cores of the copolymer structures. The solution is then filtered to remove insoluble particles. In a third method, the hydrophobic drug and the polymer are mixed in solid form and heated to about 60 C to form a melt. The melt is stirred for several hours to encourage intimate mixing of the drug and copolymer. After cooling to room temperature, the formulation is ready for immediate use or storage.

VI. Method of Using the Multi-Arm Block Copolymers As noted above, the multi-arm block copolymers of the invention can be used to solubilize hydrophobic drug molecules in aqueous solution. As a result, the copolymer structures of the invention may be used as drug delivery vehicles by entrapping the hydrophobic drug within the hydrophobic region of the copolymer and administering a therapeutically effective amount of the multi-arm block copolymer with the biologically active agent entrapped therein to a mammal.
The block copolymers of the invention can be used as drug delivery vehicles for any condition responsive to a hydrophobic drug molecule capable of entrapment within the copolymer structure. Thus, the block copolymers of the invention can be used in pharmaceutical formulations useful for treating any condition responsive to a hydrophobic drug in mammals, including humans. A preferred condition for treatment is cancer. The method of treatment comprises administering to the mammal a therapeutically effective amount of a composition or formulation containing the multi-arm block copolymer with a hydrophobic drug encapsulated therein as described above. The therapeutically effective dosage amount of any specific formulation will vary somewhat from drug to drug, patient to patient, and will depend upon factors such as the condition of the patient, the loading capacity of the block copolymer, and the route of delivery. As a general proposition, a dosage from about 0.5 to about 20 mg/kg body weight, preferably from about 1.0 to about 5.0 mg/kg, will have therapeutic efficacy. When administered conjointly with other pharmaceutically active agents, even less of the block copolymer/hydrophilic drug composition may be therapeutically effective.
The block copolymer/hydrophilic drug composition may be administered once or several times a day. The duration of the treatment may be once per day for a period of from two to three weeks and may continue for a period of months or even years. The daily dose can be administered either by a single dose in the form of an individual dosage unit or several smaller dosage units or by multiple administration of subdivided dosages at certain intervals. Possible routes of delivery include buccally, subcutaneously, transdermally, intramuscularly, intravenously, orally, or by inhalation.

VII. Experimental The following examples are given to illustrate the invention, but should not be considered in limitation of the invention. Unless otherwise indicated, all PEG
reagents are available from Shearwater Corporation of Huntsville, Alabama. All NMR data was generated by a 300 MHz NMR spectrometer manufactured by Bruker.
Materials Four 8-arm block copolymers were prepared. In each case, the poly(propylene oxide) (PPO) segments of the copolymers were covalently bonded to a hexaglycerol core through ether linkages and the poly(ethylene glycol) (PEG) moiety was covalently bound to the distal terminus of each PPO segment. Copolymer PPO-PEG
of nominal molecular weight 8500 Da was prepared with a 5300 Da PPO block and a 3200 Da PEG block. Copolymer PPO-PEG 18000 (18000 Da molecular weight) was prepared with a 5300 Da PPO block and a 12,700 Da PEG block. Copolymer PPO-PEG 16000 was prepared with a 9500 Da PPO block and a 6500 Da PEG block.
Copolymer PPO-PEG 22000 was prepared with a 9500 Da PPO block and a 12,000 Da PEG block. It should be understood that these molecular weights are an average, nominal, molecular weight for polymers having a range of molecular weights.
The general structure of a PPO-PEG copolymer of this type is given above as Formula V.
Additionally, a series of degradable multi-arm copolymers were synthesized, in which degradable poly(hydroxyesters) were used as hydrophobic segments.
These copolymers include 8-arm polylactide mPEG5kDa (8-arm PLA-mPEG5kDa), 8-arm polylactide PEG26kDa (8-arm-PLA-PEG26kDa), 8-arm polycaprolactone mPEG5kDa (8-arm-PCL-mPEG5kDa), 8-arm polycaprolactone PEG26kDa (8-arm-PCL-PEG26kDa), PEG2 attached to hydroxypropyl-(3-cyclodextrin polycaprolactone (BCD-PCL-PEG26kDa). All of the 8-arm degradable copolymers were made using a hexaglycerol core. The general structure of an 8-arm polylactide mPEG is given above as Formula VI. The 21-arm BCD-PCL-PEG26kDa copolymer comprises a hydroxypropyl-P-cyclodextrin core. As used herein, PEG2 refers to a branched PEG structure comprising two PEG backbones attached to a lysine linker, as described in U.S.
Patent No. 5,932,462. Examples 1-7 illustrate methods of synthesizing multi-arm poly(hydroxyester)-PEG copolymers.

For comparative purposes, the following additional materials were tested:
Tetronic 1037, a four arm PPO-PEG copolymer having nitrogen branching points available from BASF Corp. (Mount Olive, New Jersey); two multi-arm PEG
molecules available from NOF (Tokyo, Japan), a 4-arm copolymer comprising a pentaerythritol core and an 8-arm copolymer comprising a hexaglycerol core;
and Tween 80, a polyoxyethylene sorbitan monooleate surfactant obtained from Aldrich (Milwaukee, Wisconsin). Physical data for all tested materials is listed in Table 1 below.

Table 1. Physical data of the materials used in the experiments Polymer Mw (Da) Wt. % of PEG # of arm Non- PPO-PEG 10050 22000 54.5 8 degradable Tetronic 1307 18000 70 4 PEG l OkDa 10000 100 4 PEG l OkDa 10000 100 8 Tween 80 1310 67 N/A

PLA-mPEG5kDa 56000 71 8 PLA-PEG26kDa 64000 75 8 Degradable PCL-mPEG5kDa 56000 71 8 PCL-PEG26kDa 64000 75 8 PEG26kDa The following biologically active agents were used in the formulation and release studies detailed below: 3,4-di-[1-methyl 6-nitro-3-indolyl]-1H-pyrrole-2,5-dione (MNIPD) (available from F. Hoffmann-La Roche Ltd, Basel, Switzerland), simvastatin (available from Merck & Co., Inc., Whitehouse Station, NJ, USA), indomethacin (available from Sigma, St. Louis, MO, USA), pivaloyloxymethyl butyrate (available from Titan Pharmaceuticals, Inc., San Francisco, CA, USA), cyclosporin A (available from Fluka, Milwaukee, WI, USA), and paclitaxel (available from LKT laboratories, Inc., St. Paul, Minnesota, USA).
Drug Loading Methods Three methods were used to load a hydrophobic drug into the multi-arm block copolymer formulations. Method I utilized an organic solvent. Method II
utilized an aqueous solution. Method III was performed in the absence of a solvent.
Method I: The hydrophobic drug and the copolymer were co-dissolved in methylene chloride. The solution was air-dried overnight and then dried under vacuum. The resulting solid was either stored at -20 C for future use after thawing, dissolving in buffer, and filtering, or it was dissolved immediately in a buffer, filtered to remove insoluble particles and the filtrate frozen and stored at -20 C.

Method II: The hydrophobic drug was suspended in a buffered polymer solution. The suspension was subjected to ultrasonication for about three hours, and then filtered through a 0.2 pm syringe filter. The filtrate was frozen and stored at -C.
Method III: The hydrophobic drug and the polymer were placed in a capped 20 vial under argon and heated to 60 C to form a melt. The melt was stirred for two hours using a magnetic stirrer. After cooling to room temperature, the formulation was ready for immediate use or storage for future use.

Example 1 Preparation of 8-arm Polylactide-mPEGSkDa (8-arm-PLA-mPEG5kDa) In a 250 mL three neck round bottom flask, hexaglycerol (4.307gm, 0.008 moles (Sakamoto Yakuhin Kogyo Co., Ltd., Osaka, Japan) was heated at 100 C for one hour under vacuum (I mmHg). The contents were cooled to ambient temperature and placed under argon. DL-lactide (160 gm, 1.110 moles (Purasorb, Purac, Holland) was added and the flask flushed with argon then heated at 150 C. Stannous 2-ethyhexaneoate (94.6 mg, 2.22x 10-4 moles) was added and the mixture heated under argon at 170 C for twenty-four hours. The mixture was cooled to 160 C and stirred under vacuum (less than lmm Hg) for three hours. After cooling to room temperature the mixture was dissolved in dichloromethane (900 mL). The solution was concentrated to near dryness at reduced pressure and poured into hexanes (1500 mL) with stirring to precipitate. The supernatant was decanted and the residue dried under vacuum. NMR (CDC13): S 5.16 (m, -OCH(-CH3)CO-), 1.57 (d, ill resolved, -OCH(-CH3)CO-).
In a round-bottom flask, 8-arm PLA prepared from above (2 grams), mPEG5k-CM (5 grams), 1-hydroxybenzotriazole (HOBT, 65 mg), 4-(dimethylamino)pyridine (DMAP, 120 mg) and dicyclohexylcarbodiimide (DCC, 288 mg) were mixed with 40 ml of anhydrous methylene chloride. The mixture was stirred at room temperature overnight, the insoluble solid was removed by filtration, and the solvent was evaporated under reduced pressure. The residue was added to 100 ml of ether and the resulting precipitate was collected by filtration and dried under vacuum.
Yield: 5.5 g (78%). 'H NMR(DMSO-d6): 8 3.5 (br m, PEG), 4.20 (s, -PEG-OCH2COO-PLA), 5.16 (m, -OCH(-CH3)CO-), 1.45 (d, ill resolved, -OCH(-CH3)CO-).
Example 2 Preparation of 8-arm Polylactide PEG26kDa (8-arm-PLA-PEG2kD~
8-arm-polylactide (8-arm-PLA) (3.00 g, Mw -20 kDa), branched PEG
carboxylic acid (PEG2-COOH, 6kDa, 7 g), DMAP (120 mg), HOBT (105 mg) and DCC (440 mg) were dissolved in methylene chloride (50 ml). The reaction was stirred at room temperature for about 72 hours. The solvent was then removed under vacuum, and 35 ml of 1,4 dioxane was added to the syrup. After filtering, the filtrate was added to 200 ml of diethyl ether. The precipitate was collected by filtration, washed with isopropyl alcohol (IPA) and ether, and then dried overnight under vacuum. Yield: 9.4 g. 'H NMR(DMSO-d6): S 3.5 (br m, PEG), 1.45 (d, -OCCH(CH )O-), 5.165 (m, OCCH(CH3)O-), 4.03 (t, mPEGOCHzCH OCONH-).

Example 3 Preparation of 8-arm s-Polycaprolactone (8-arm PCL) Hexaglycerol (2.156 g) was dried by heating at 100 C for 16 hours under vacuum. Five ml of N, N-dimethyl formamide was added and the mixture heated under argon to 80 C. To the resulting mixture was added 80 g (74 ml) of s-caprolactone (Aldrich) and stannus ethyl hexaneoate (48 mg). The mixture was heated to 110 C for -72 h. The flask was cooled and unreacted reagent and solvent were removed under vacuum. Yield: -80g.'H NMR(DMSO-d6): 6 1.23 (br m, -OCCH2CH2CH2CH2CH2O-), 1.52 (m, -OCCH2CHZCHZCH2CH2O-), 2.26 (t, -OCCHZCH2CH2CH2CH2O-), 3.98 (t, -OCCH2CH2CHZCH2CH2O-). The molecular weight of the 8-arm-PCL was estimated as 16000 Dalton by NMR and GPC.
Example 4 Preparation of 8-arm Polycaprolactone mPEG5kDa (8-arm-PCL-mPEG5kDa) 8-arm-PCL from Example 3(1.00 g), carboxymethyl mPEG5kDa (2.10 g), DMAP (60 mg), HOBT (35 mg) and DCC (140 mg) were dissolved in methylene chloride (30 ml). The reaction was stirred at room temperature for 46 hours.
The solvent was then removed under vacuum, and 15 ml of 1,4 dioxane was added to the syrup. After filtering, the filtrate was concentrated by removing excess 1,4 dioxane under vacuum. The product was precipitated with 200 ml of diethyl ether, stirred for 5 minutes, and collected by filtration. The product was dried overnight under vacuum.
Yield: 2.6 g. 'H NMR(DMSO-d6): S 3.5 (br m, PEG), 4.20 (s, -PEG-OCH2COO-PCL), 1.28 (br m, -OCCH2CH2CH2CH2CH2O-), 1.55 (m, -OCCH2CH2CH2CH2CHZO-), 2.26 (t, -OCCH2CH2CH2CH2CHZO-), 3.99 (t, -OCCH2CH2CH2CH2CH2O-).
Example 5 Preparation of 8-arm Polycaprolactone PEG26kDa (8-arm-PCL-PEG26kDa~
8-arm-PCL (1.00 g), branched PEG carboxylic acid (PEG26kDa COOH, 2.52 g), DMAP (60 mg), HOBT (35 mg) and DCC (140 mg) were dissolved in methylene chloride (30 ml). The reaction was stirred at room temperature for about 72 hours.
The solvent was then removed under vacuum, and 15 ml of 1,4 dioxane was added to the syrup. After filtering with celite, the filtrate was concentrated by removing excess 1,4 dioxane under vacuum. The product was precipitated with 200 ml of diethyl ether, stirred for 5 minutes, collected by filtration, and dried overnight under vacuum. Yield:
3.1 g'H NMR(DMSO-d6): 8 3.5 (br m, PEG), 1.28 (br m, -OCCH2CH2CH2CH2CH2O-), 1.55 (m, -OCCH2CH2CH2CH2CH2O-), 2.26 (t, -OCCHZCH2CH2CH2CH2O-), 3.99 (t, -OCCH2CH2CH2CH2CH2O-).

Example 6 Preparation of Polycaprolactone Initiated with Hydroxypropyl-(3-cyclodextrin (BCD-PCL
Hydroxypropyl-(3-cyclodextrin (BCD; 100 substitution) was purchased from Aldrich and used as received. c-Caprolactone (CL; Aldrich) was purified by dehydration with CaH2 and distillation under vacuum. The purified product was stored under N2 atmosphere at -20 C until use. Stannous 2-ethyhexaneoate (SnOct;
Aldrich) and all other reagents were used as received.

Hydroxypropyl-(3-cyclodextrin (BCD, 1.45 gram, 1 mmol) was vacuum-dried in a round-bottomed flask at 100 C for 1 hour and purged with dry N2. Purified s-caprolactone (42 gram, 0.368 mol) was added to the flask using a syringe.
Thirty-two milligrams of stannous 2-ethyhexaneoate (SnOct, Aldrich) was added, and the mixture stirred for 24 hours. The mixture of reagents became viscous without significant change in color. While the mixture was cooled, tetrahydrofuran (100 ml) was added. The polymer was precipitated by addition of about 2 L of isopropanol (IPA). The precipitate was collected by filtration and redissolved in benzene and freeze-dried for 2 days. Yield: 37 g (86 %).

Example 7 Preparation of PEG2 attached to BCD-PCL (BCD-PCL-PEG26kQ
One gram of BCD-PCL was mixed with 3.8g (0.025 mmol) of PEG2-carboxylic acid (MW6,000), 0.866g of dicyclohexyl carbodiimide (4.2mmol), 0.122g of 4-dimethylaminopyridine (1.0 mmol) and 0.068 g of hydroxybenzotriazole (0.5mmo1) in 20 ml of 1,2-dichloroethane (or dichloromethane), and stirred for hours. The solvent was removed under vacuum, and the remaining gummy material was dissolved in 40 ml of 1,4-dioxane. The undissolved material was removed by filtration and the solution was added to 400 ml of diethylether. The precipitate was filtered and dried under vacuum for 48 h. Yield: 4.2 gram (88% ).

Example 8 Synthesis of Bisphosphonate Derivative of Multi-Arm PPO-PEG
8 arm PPO-PEG(18KDa)(succinimidyl carbonate)8:
8 arm PPO-PEG (18KDa) (15.0 g, -0.83 mmol) in acetonitrile (200 mL) was treated with disuccinimidyl carbonate (DSC) (1.9 g, 7.4 mmol) and pyridine (0.70 ml). The reaction was stirred overnight at room temperature under an argon atmosphere. The reaction was concentrated to dryness and the residue was dissolved in dichloromethane (-200 ml). The clear solution was washed with a 10%
solution of sodium phosphate, sodium chloride (2 x 200 ml). The organic layer was dried (Na2SO4), filtered, and the solvent was removed to afford 8 arm PPO-PEG
(18KDa)-((x-succinimidyl carbonate)8 (15.0 g, -100%).

8 arm PPO-PEG(18KDa)(AHBDP)5:
8 arm PPO-PEG (18KDa)-(a-succinimidyl carbonate)8 (10.0 g, -0.55 mmol) and 4-amino-l-hydroxybutane-1,1-diphosphonic acid, ditetrabutylammonium salt (AHBDP) (2.96 g, 3.76 mmol) were dissolved in acetonitrile (200 ml) and treated with triethylamine (0.8 ml, 5.74 mmol). The clear, colorless solution was stirred overnight under and argon atmosphere. The solution was concentrated to dryness, the residual gum dissolved in water (100 mL), and the pH was adjusted to 11. The basic solution was stirred at room temperature for 2 h. The solution was then adjusted pH
7.0 with HCl and passed through an IR 120 column (75 ml). The water was removed in vacuo at ca. 50 C to afford the product as a gum. Further drying in vacuo followed by trituration with CH2C12 with Et20 afforded the product as a waxy solid (4.5 g). 'H NMR (dmso-d6, 300 MHz) S 1.04 (d, 280 H, OCH(CH3)CHZ), 1.59-1.76 (m, 12.5H, OCONHCHZCHZCHZ), 1.76-1.94 (m, 12.4H, OCONHCH2CH2CH2), 2.88-2.99 (m, 12.9H, OCONHCH2CH2CH2), 3.51 (bs, 1191 H, PEG backbone), 4.03 (t, 13H, J4.4 Hz, CH2CH2OCONH), 7.16 (t, 5.OH, J 5.1 Hz, CH2CH2OCONH).
Example 8 illustrates a method of synthesizing a multi-arm copolymer including a targeting moiety attached to a distal end of the outer PEG
polymer.

Example 9 Preparation of Cyclosporin A-Loaded 8-Arm-PCL-PEG26kDa In a glass vial, 6 mg of cyclosporin A and 60 milligram of 8-arm-PCL-PEG26kDa (drug/polymer weight ratio 1/10) were dissolved in 1 ml of methylene chloride. The solution was dried under argon. The dried solid was heated at 55 C for two hours under argon. The melt was then cooled to room temperature, placed under vacuum overnight, and reduced to small particles. To the particles was added 1 ml of phosphate buffer (0.1 M, pH 7.0), and the resulting mixture was filtered through 0.2 m syringe filter. Cyclosporin A concentration was 5.5 mg/ml by HPLC.

Example 10 Preparation of Paclitaxel-Loaded 8-Arm-PCL-PEG26kDa Paclitaxel (6 mg) and 8-arm-PCL-PEG26kDa (60 mg) (drug/polymer weight ratio 1/10) were dissolved in 1 ml of methylene chloride. The solution was dried under argon. The dried solid was heated at 55 C for two hours under argon.
The melt was then cooled to room temperature and placed under vacuum overnight, and reduced to small particles. To the particles was added 1 ml of phosphate buffer (0.1 M, pH 7.0). The resulting mixture was filtered through 0.2 m syringe filter.
Paclitaxel concentration was above 4.5 mg/ml by HPLC.
Example 11 Solubility of Drugs in PPO-PEG Multi-Arm Block Copolymers For several drug molecules, 50 mg of PPO-PEG block copolymer 10050/drug formulation with 10 wt. % of drug loading was dissolved in I ml of phosphate buffer (0.1 M, pH 7.4). After two hours of mixing, the mixture was filtered through 0.2 m syringe filter. The drug concentration in the filtrate was determined by HPLC
or UV
using a standard curve. The results are listed in Table 2. As noted in Table 2, in each case, incorporation of the drug into the multi-arm PPO-PEG block copolymer greatly increased the solubility of the drug in buffer solution.

Table 2. Solubility of Drug in 50 mg of the Multi-Arm PPO-PEG Block Copolymer (phosphate buffer, 0.1 M, pH 7.4).

Drug MNIPD Simvastatin Indomethacin Paclitaxel Pivaloyloxymethyl butyrate Solubility of < 0.5 < 1 g/ml 88 g/ml < 1 g/ml -Drug in g/ml Plain Buffer Solubility of 2.6 4 mg/ml 4 mg/ml 2 mg/ml 12 mg /ml Drug in mg/ml Copolymer/
Drug Formulation Solubility of -5,000 -4,000 -45 -2,000 -Formulation Relative to Solubility in Buffer Example 12 Degradation Studies of Degradable Multi-Arm Block Copolymers Each of the multi-arm PEG block copolymers having degradable hydrophobic segments was dissolved in either phosphate buffer (0.1 M, pH 7.0) or rat serum to a final concentration of 1-4 wt. %. The solution was placed in an incubator at 37 C.
The concentrations of the copolymer and free PEG were monitored at timed intervals by HPLC. For the solution in rat serum, the copolymer and PEG were first extracted with methylene chloride and then analyzed by HPLC, while for the solution in buffer, analysis was done directly by HPLC. Half-lives (tl/2) were calculated based on first order kinetics, as shown in Table 3. The data indicate that all of the tested polymers are degradable in both rat serum and phosphate buffer with varying degradation rates depending on the structure of the polymer. Larger PEG segments tended to result in longer degradation half-lives.

Table 3. Degradation Half-Lives (tl/2) of Selected Multi-Arm Block Copolymers Sample In phosphate buffer (pH 7.0) In rat serum 8-arm-PLA-PEG5k 365 h 7 h 8-arm-PLA-PEG26kDa 1251 h 8 h 8-arm-PCL-PEG5k 643 d 170 h 8-arm-PCL-PEG26kDa 1010 d 507 h Example 13 Drug Release Studies Using PPO-PEG Multi-Arm Block Copolymers In aqueous media, complexes of soluble lipophilic/hydrophobic drugs with the multi-arm PPO-PEG copolymer slowly release the drug. The water-insoluble drugs precipitate out of the formulation over time. Release profiles of the drugs were studied by determining the concentration of solubilized drug as a function of time at 23 C. At each time interval, aliquots were filtered through a 0.2 m syringe filter and concentrations of drug measured by rp-HPLC or UV methods. For example, release of MNIPD from the soluble MNIPD/PPO-PEG formulation were measured by withdrawing 100 l of solution, diluting to 1000 1 in water (at which point the drug dissolves), filtering through a 0.2 m filter, and measuring absorbance of the filtrate at 465 nm. Drug release curves are presented in Figures 2-7.
In Figure 2 is shown a comparison of the release rate of the drug, MNIPD, from PPO-PEG multi-arm copolymers 6035 and 6070 with the release rate of MNIPD
from multi-arm PEGs (4-arm and 8-arm) and from Tween 80 (see Table 1). MNIPD
was loaded into the polymers by Method I. After the MNIPD/polymer formulation was dissolved in phosphate buffer (0.1 M, pH 7), the release of the drug was followed by UV at 465 nm. The drug was released more slowly from copolymers 6035 and 6070 than from Tween 80. Solubility in the 4- and 8-arm PEGs was low and the drug was rapidly released from these polymers.
Release profiles of simvastatin from seven polymers are shown in Figure 3.
Simvastatin was loaded into the polymers by Method I. After dissolving in phosphate buffer (0.1 M, pH 7 ), the release of the drug was followed by HPLC. An extended release profile was observed from block copolymers 10050 and 10037 (see Table 1), while release from the PEGs (4-arm and 8-arm), 1307 and PPO-PEG 6035 was significantly more rapid. Solubility in the multi-arm PEG molecules and in PPO-PEG
6070 was very low.
In Figure 4 is shown a release profile for simvastatin from PPO-PEG
copolymer 10050 having a bisphosphonate targeting group attached to a distal terminus of the PEG moiety of the copolymer. Simvastatin was loaded into the bisphosphonate derivative of the copolymer by method I. After the drug/copolymer formulation dissolved in phosphate buffer (0.1 M, pH 7), the release of the drug was followed by HPLC. The drug was released over about 80 hours.
In Figure 5 is shown a comparison of release profiles of paclitaxel from copolymer 10050 and from 8-arm PLA-PEG block copolymer. Paclitaxel was loaded into the copolymers by method I. After dissolving in phosphate buffer (0.1 M, pH 7), the release of the drug was followed by HPLC. Higher drug loading was possible with the 8-arm PLA-PEG copolymer.
In Figure 6 is shown a comparison of release profiles of indomethacin from various copolymers. Indomethacin was loaded into the copolymers by Method I.
After dissolving in phosphate buffer (0.1 M, pH 7), the release of the drug was followed by HPLC. Drug solubility was enhanced by the multi-arm block copolymers as well as by multi-arm PEG. Little or no release was observed from any of the polymers.
In Figure 7 is shown comparative release profiles for pivaloyloxymethyl butyrate at two concentrations in PPO-PEG copolymers 10050 and 10037.
Pivaloxymethyl butyrate was loaded into the copolymers by Method III. After dissolving in phosphate buffer (0.1 M, pH 7), the release of the drug was followed by HPLC. Extended release was observed from both polymers.
Example 14 Release Profile of Cyclosporin A from Degradable 8-arm-PCL-PEG26kDa The solution prepared in Example 9 was incubated at 37 C. At timed intervals, 10 l of sample was withdrawn and diluted with phosphate buffer (0.1 M, pH 7.0). The solution was filtered through 0.2 m syringe filter. The filtrate was analyzed by rp-HPLC for cyclosporin A concentration. The soluble cyclosporin A
concentration in solution vs. time is shown in Figure 8. The data illustrate the ability of the block copolymer to retain the cyclosporin A in solution for an extended period of time and to provide a controlled release of the drug.

Example 15 Release Profile of Paclitaxel from Degradable 8-arm-PCL-PEG26kDa The solution prepared in Example 10 was incubated at 37 C. At timed intervals, 10 l of sample was withdrawn and diluted with phosphate buffer (0.1 M, pH 7.0). The solution was filtered through 0.2 m syringe filter. The filtrate was analyzed by rp-HPLC for paclitaxel concentration. The soluble paclitaxel concentration in solution vs. time is shown in Figure 9. The data illustrate the ability of the block copolymer to retain the paclitaxel in solution for an extended period of time and to provide a controlled release of the drug.

Example 16 Antitumor Study of Paclitaxel-Loaded 8-arm PLA-mPEG5kDa in NCI-H460 Non-small Cell Lung Tumor Xenograft in Mice NCI-H460 non-small cell lung tumor was implanted subcutaneously in athymic nude mice. After the tumor grew to approximately 175 mg, the aqueous formulation of paclitaxel-loaded 8-arm PLA-mPEG5kDa was injected into mice via the tail vein. The mice were observed daily for survival. Tumor weights and body weights were recorded twice weekly. Each tumor was measured by caliper in two dimensions and converted to tumor mass using the formula for a prolate ellipsoid.
For comparison, a standard formulation of Taxol (in Cremophor ) and a control were also used. The results are shown in Figure 10. The data indicate that the inhibition of tumor growth exhibited by the 8-arm PLA-mPEG block copolymer/drug formulation (referred to as UM-Paclitaxel in Figure 10) was comparable to the standard Taxol formulation.

Example 17 Tolerance Study of Multi-Arm Block Copolymers in Mice Dosages ranging from 500 to 2000 mg/kg/dose were intravenously administered to athymic nude mice for five days (days 1-5). As indicated in Table 4, all dosages were well tolerated.

Table 4. Tolerance study of multi-arm copolymers Unimolecular micelle Mean animal 21-day polymers weight loss survival during 21 days 8-arm-PLA-PEG5k < 5% All 8-arm-PLA-PEG26k < 5% All 8-arm-PCL-PEG26k < 5% All Example 18 Dynamic Light Scattering Study Micelle preparation:
A block copolymer (0.14g) selected from the group including linear PEG-PLA, 8-arm-PLA-PEG5k, 8-arm-PLA-PEG26kDa, 8-arm-PCL-PEG5k, and 8-arm-PCL-PEG26kDa was dissolved in 20 ml of N-dimethylacetamide (DMAc). The solution was warmed in order to dissolve the polymer easily. The solution was put into the pre-swollen dialysis membrane (Spectra/Prol, MWCO 6000-8000) after 0.2 m filtration. Dialysis was carried out against deionized water for 24 h. Water was changed at 1, 2, 4 and 7 hours from the beginning. The prepared micelle solution was stored at 4 C until use.

Micelles loaded with paclitaxel:

Taxol was loaded into the micelle solution in two ways. Method 1: To the micelle solution (10 ml) prepared as described above was added 0.5 ml of paclitaxel solution in CHC13 (4mg/mL) dropwisely. After 16 h of vigorous stirring, CHC13 was removed from the solution by aspiration. The solution was filtered through 0.2 m membrane. Method 2: Block copolymer (0.14g) and paclitaxel (5mg) were dissolved in DMAc and dialyzed as described above. After dialysis, the solution was filtered with 0.2 m membrane.

Dynamic Light Scattering (DLS):
The size and the distribution of micelles were measured by dynamic light scattering. The sample was filtered with 0.2 m pore-size membrane prior to the measurement. The measurement was carried out at 25 C. Size and polydispersity of the particle was determined by cumulant analysis method based on the assumption that the micelles were spherical. Figure 11 provides an example of dynamic light scattering results. Tables 5, 6 and 7 provide micelle sizes and polydispersity for micelles with and without paclitaxel loading as determined by light scattering.

Table 5. Size of Micelles Determined by Light Scattering Sample Effective Polydispersity Count Rate Diameter (nm) Linear PEG-PLA 30.4 0.198 313.8 8-arm-PLA-PEG5k 47.0 0.291 123.5 8-arm-PLA-PEG26kDa 100.7 0.351 251.9 8-arm-PCL-PEG5k 24.9 0.066 101.2 8-arm-PCL-PEG26kDa 19.3 0.079 73.3 Table 6. Size of Micelles Loaded with Paclitaxel Prepared by Method I
Sample Effective Polydispersity Count Rate Diameter (nm) Linear PEG-PLA 39.1 0.226 363.0 8-arm-PLA-PEG5k 8-arm-PLA-PEG26kDa 8-arm-PCL-PEG5k 49.0 0.106 692.0 8-arm-PCL-PEG26kDa 26.2 0.177 153.3 Table 7. Size of Micelles Loaded with Paclitaxel Prepared by Method 2 Sample Effective Polydispersity Count Rate Diameter (nm) Linear PEG-PLA 31.8 0.246 312.3 8-arm-PLA-PEGSk 8-arm-PLA-PEG26kDa 86.4 0.342 235.7 8-arm-PCL-PEG5k 8-arm-PCL-PEG26kDa 19.7 0.064 77.3 The above data indicate that the effective diameter of the multi-arm block copolymer structure increases after loading with the drug.
Example 19 Evaluation of Micelle Aggregates by Dynamic Light Scattering Micelle solutions of BCD-PCL-PEG26kDa, 8-arm-PCL-PEG26kDa, and linear PEG-PCL (MW 5,000-5,000) were prepared by a dialysis method. For linear PEG-PLA, the polymer solution in DMAc was mixed with water by dropwise adding 20 mL of water to the polymer solution prior to the dialysis in order to avoid the formation of aggregation. The concentration of micelle solutions was in the range of 2.88 - 3.34 mg/ml (see Table 8).
The micelle solutions were treated with -2.5 ml of 5% SDS solution for 24 hours. The micelle solutions before and after the addition of surfactant (SDS) were characterized after filtration through 0.2 m syringe filter using a Brookhaven 90 Plus Particle Sizer. Table 4 summarizes the DLS cumulant analysis results. The cumulant diameter of the micelles ranged from 19 to 35 nm before the SDS addition. The DLS
measurement was also carried out without filtration, and little alteration of micelle property was seen on the multi-arm block copolymers. BCD-PCL-PEG26kDa had the least change in micelle property before and after SDS addition. The other micelles presented dramatic change in size and significant decrease in count rate. The count rate of the BCD-PCL-PEG26kDa micelles was reduced by 30%. This was likely due to the dilution of the micelle solution rather than the dissociation of micelles.
Multi-armed PEG block copolymers with higher number of arms tend to be less aggregated.

The data tends to suggest that increases in the number of arms of the multi-arm block copolymers of the present invention reduces the tendency of the hydrophobic cores of the copolymers to aggregate in the same manner as conventional linear micelles. Since less aggregation occurs with multi-arm copolymers with a greater number of arms, less disaggregation is caused by addition of the surfactant. In contrast, smaller block copolymers of the invention and linear micelles tend to aggregate to a greater extent, thereby resulting in a measurable disruption in aggregation by the surfactant.

Table 8. DLS cumulant analysis results of BCD-PCL--(PEG3k)2, 8-arm-PCL-PEG26kDa, and linear PEG-PCL micelles Concentration Count rate % Count Samples (mg/mL) Diameter (nm) Dispersity (kcps) Remaining BCD-PCL-PEG26kDa 2 Before filter 3.34 19.8 0.098 52.7 BCD-PCL-PEG26kDa After filter 3.34 19.4 0.058 44.2 BCD-PCL-PEG26kDa +
SDS 3.34 22.7 0.152 30.7 69.45701357 8-arm-PCL-PEG26kDa Before filter 3.24 8-arm-PCL-PEG26kDa After filter 3.24 26.8 0.158 107.1 8-arm-PCL-PEG26kDa +
SDS 3.24 42.2 0.24 23.5 21.94211018 Linear PEG-PCL
Before filter 2.88 Linear PEG-PCL
After filter 2.88 35.6 0.006 348.7 Linear PEG-PCL + SDS 2.88 11 0.401 11 3.154574132 Many modifications and other embodiments of the invention will come to mind to one skilled in the art to which this invention pertains having the benefit of the teachings presented in the foregoing descriptions and the associated tables.
Therefore, it is to be understood that the invention is not to be limited to the specific embodiments disclosed and that modifications and other embodiments are intended to be included within the scope of the appended claims. Although specific terms are employed herein, they are used in a generic and descriptive sense only and not for purposes of limitation.

Claims (32)

WHAT IS CLAIMED IS:
1. A pharmaceutical composition, comprising:
a multi-arm block copolymer, the block copolymer comprising a central core molecule comprising a residue of a polyol, and at least three copolymer arms covalently attached to the central core molecule, each copolymer arm comprising an inner hydrophobic polymer segment covalently attached to the central core molecule and an outer hydrophilic polymer segment covalently attached to the hydrophobic polymer segment, wherein the central core molecule and the hydrophobic polymer segment define a hydrophobic core region, and at least one biologically active agent entrapped within the hydrophobic core region of the multi-arm block copolymer.
2. The pharmaceutical composition of claim 1, wherein the biologically active agent is selected from the group consisting of 3,4-di-[1-methyl 6-nitro-3-indoly]-1H-pyrrole-2,5-dione (MNIPD), simvastatin, indomethacin, pivaloyloxymethyl butyrate, cyclosporin A, paclitaxel, analogs thereof, and pharmaceutically acceptable salts thereof.
3. The pharmaceutical composition of claim 1 or claim 2 wherein the outer hydrophilic polymer segment comprises poly(ethylene glycol).
4. The pharmaceutical composition of any one of claims 1 to 3, wherein the multi-arm block copolymer has the structure:
(E-C-O-B-O-)p A(-O-B-O-C-D)m wherein:

A is the central core molecule, O is oxygen, B is the inner hydrophobic polymer segment, C is the outer hydrophilic polymer segment, D is a hydroxyl or alkyl group, P is at least 1, the sum of m and p is from 3 to about 25, and E is a functional group selected from the group consisting of active ester, active carbonate, acetal, aldehyde, aldehyde hydrate, alkyl or aryl sulfonate, halide, disulfide, alkenyl, acrylate, methacrylate, acrylamide, active sulfone, amine, protected amine, hydrazide, protected hydrazide, thiol, protected thiol, carboxylic acid, protected carboxylic acid, isocyanate, isothiocyanate, maleimide, vinylsulfone, dithiopyridine, vinylpyridine, iodoacetamide, epoxide, glyoxal, dione, mesylate, tosylate, and tresylate.
5. The pharmaceutical composition of any one of claims 1 to 4, wherein the central core molecule is a residue of a polyol selected from the group consisting of glycerol, sorbitol, pentaerythritol, glycerol oligomers and hydroxypropyl-.beta.-cyclodextrin.
6. The pharmaceutical composition of any one of claims 1 to 5, wherein the inner hydrophobic polymer segment is selected from the group consisting of a poly(hydroxyester), a poly(alkylene oxide) other than poly(ethylene glycol), and copolymers thereof.
7. The pharmaceutical composition of claim 6 wherein the inner hydrophobic polymer segment is selected from the group consisting of poly(lactide), poly(glycolide), poly(lactide)/(glycolide) copolymer, poly(butyrolactide), and polycaprolactone, polypropylene oxide and polybutylene oxide.
8. The pharmaceutical composition of any one of claims 1 to 7, wherein the hydrophobic polymer segment has a molecular weight of about 10,000 Da to about 40, 000 Da.
9. The pharmaceutical composition of any one of claims 1 to 8, wherein the central core molecule is attached to at least five copolymer arms, at least 8 copolymer arms or at least 10 copolymer arms.
10. The pharmaceutical composition of any one of claims 1 to 3 wherein the multi-arm block copolymer has the structure:
A(-O-B-O-C-D)n wherein:
A is the central core molecule, O is oxygen, B is the inner hydrophobic polymer segment, C is the outer hydrophilic polymer segment, D is a capping group, and N is 3 to about 25.
11. The pharmaceutical composition of claim 10 wherein each D is C1-C6 alkoxy or hydroxyl.
12. The pharmaceutical composition of claim 10, wherein C comprises a hydrolysable linkage selected from the group consisting of ester, carbonate, imine, hydrazone, phosphate esters, orthoester, peptide or acetal.
13. The pharmaceutical composition of any one of claims 3 to 9 wherein at least one capping group is covalently attached to at least one hydrophilic polymer segment.
14. The pharmaceutical composition of any one of claims 10 to 13 wherein the capping group is selected from the group consisting of alkoxy, hydroxyl, protected hydroxyl, active ester, active carbonate, acetal, aldehyde, aldehyde hydrate, alkyl or aryl sulfonate, halide, disulfide, alkenyl, acrylate, methacrylate, acrylamide, active sulfone, amine, protected amine, hydrazide, protected hydrazide, thiol, protected thiol, carboxylic acid, protected carboxylic acid, isocyanate, isothiocyanate, maleimide, vinylsulfone, dithiopyridine, vinylpyridine, iodoacetamide, epoxide, glyoxal, dione, mesylate, tosylate, and tresylate.
15. The pharmaceutical composition of claim 1 wherein each hydrophobic and hydrophilic polymer segment has a molecular weight of about 500 Da to about 100,000 Da.
16. The pharmaceutical composition of claim 1 wherein the hydrophilic polymer segment has a molecular weight of about 1,000 Da to about 20,000 Da.
17. The pharmaceutical composition of any one of claims 1 to 16, wherein each copolymer arm is identical.
18. The pharmaceutical composition of any one of claims 1 to 3 wherein the multi-arm block copolymer has the structure:
(T-C-O-B-O-)p A(-O-B-O-C-D)m wherein:
A is the central core molecule, O is oxygen, B is the inner hydrophobic polymer segment, C is the outer hydrophilic polymer segment, D is a capping group, P is at least 1, the sum of m and p is from 3 to about 25, and T is a targeting moiety.
19. The pharmaceutical composition of any one of claims 1 to 17 wherein at least one targeting moiety is covalently attached to at least one hydrophilic polymer segment.
20. The pharmaceutical composition of claim 18 or claim 19 wherein the targeting moiety is a bisphosphonate or is selected from the group consisting of a protein, an antibody, an antibody fragment, a peptide, a carbohydrate, a lipid, an oligonucleotide, DNA, RNA, and a small molecule having a molecular weight less than 2,000 Daltons.
21. The pharmaceutical composition of any one of claims 1 to 20, wherein at least one of the hydrophobic and hydrophilic polymer segments comprises at least one degradable linkage.
22. The pharmaceutical composition of any one of claims 1 to 21, wherein the central core molecule is a residue of hexaglycerol or hydroxypropyl-.beta.-cyclodextrin.
23. The pharmaceutical composition of any one of claims 1 to 22, wherein branching is present in either the hydrophobic or hydrophilic polymer segment.
24. The multi-arm block copolymer as defined in any one of claims 18 to 23.
25. A method of preparing the pharmaceutical composition defined in any one of claims 1 to 23 comprising:
providing the multi-arm block copolymer defined in any one of claims 1 to 23, and entrapping said biologically active agent within the hydrophobic core region of the multi-arm block copolymer.
26. The method of claim 25 wherein said entrapping step comprises dissolving the multi-arm block copolymer and biologically active agent in an organic solvent to form a mixture and drying the mixture to form a solid pharmaceutical composition; or suspending the biologically active agent in an aqueous solution of the multi-arm block copolymer and subjecting the solution to ultrasonication; or mixing the biologically active agent and multi-arm block copolymer in solid form, heating the mixture to form a melt, and stirring the mixture to intimately mix the biologically active moiety and the multi-arm block copolymer.
27. The pharmaceutical composition according to any one of claims 1 to 23 for veterinary or human medical use.
28. The pharmaceutical composition according to claim 27, wherein said composition is for oral, buccal, rectal, topical, nasal, ophthalmic or parenteral administration or for administration by an inhalation route.
29. A use of the pharmaceutical composition defined in any one of claims 1 to 23 in the manufacture of a medicament for veterinary or human medical use.
30. The use according to claim 29, wherein the medicament is for oral, buccal, rectal, topical, nasal, ophthalmic or parenteral administration or for administration by an inhalation route.
31. A use of the pharmaceutical composition defined in any one of claims 1 to 23 for veterinary or human medical use.
32. The use according to claim 31, wherein the medicament is for oral, buccal, rectal, topical, nasal, ophthalmic or parenteral administration or for administration by an inhalation route.
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Families Citing this family (201)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040009229A1 (en) * 2000-01-05 2004-01-15 Unger Evan Charles Stabilized nanoparticle formulations of camptotheca derivatives
US7265186B2 (en) * 2001-01-19 2007-09-04 Nektar Therapeutics Al, Corporation Multi-arm block copolymers as drug delivery vehicles
TWI246524B (en) * 2001-01-19 2006-01-01 Shearwater Corp Multi-arm block copolymers as drug delivery vehicles
US20040185101A1 (en) * 2001-03-27 2004-09-23 Macromed, Incorporated. Biodegradable triblock copolymers as solubilizing agents for drugs and method of use thereof
US7009033B2 (en) * 2001-07-02 2006-03-07 Polymer Source Inc. Heterofunctional polyethylene glycol and polyethylene oxide, process for their manufacture
US7649023B2 (en) * 2002-06-11 2010-01-19 Novartis Ag Biodegradable block copolymeric compositions for drug delivery
KR20040040782A (en) * 2002-11-08 2004-05-13 선바이오(주) Novel hexa-arm polyethylene glycol and its derivatives and the methods of preparation thereof
KR20130135986A (en) 2003-03-05 2013-12-11 할로자임, 아이엔씨 Soluble hyaluronidase glycoprotein (shasegp), process for preparing the same, uses and pharmaceutical compositions comprising thereof
US7871607B2 (en) * 2003-03-05 2011-01-18 Halozyme, Inc. Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminoglycanases
US20060104968A1 (en) 2003-03-05 2006-05-18 Halozyme, Inc. Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminogly ycanases
JP2005008614A (en) * 2003-03-28 2005-01-13 Nano Career Kk Drug delivery system for ophthalmology by using macromolecular micelle
US7482376B2 (en) 2003-07-03 2009-01-27 3-Dimensional Pharmaceuticals, Inc. Conjugated complement cascade inhibitors
TWI248947B (en) * 2003-07-21 2006-02-11 Ind Tech Res Inst Biodegradable copolymer, and polymeric micelle composition containing the copolymer
EP1525890A1 (en) 2003-10-02 2005-04-27 Complex Biosystems GmbH Protein-Proteophore complexes
JP4235226B2 (en) * 2003-10-10 2009-03-11 サムヤン コーポレイション Amphiphilic block copolymer and polymer composition for drug delivery comprising the same
US20050214250A1 (en) 2003-11-06 2005-09-29 Harris J M Method of preparing carboxylic acid functionalized polymers
EP1718664A4 (en) * 2004-02-02 2008-08-13 Ambrx Inc Modified human four helical bundle polypeptides and their uses
US7354980B1 (en) 2004-03-12 2008-04-08 Key Medical Technologies, Inc. High refractive index polymers for ophthalmic applications
US7588745B2 (en) * 2004-04-13 2009-09-15 Si Options, Llc Silicon-containing products
EP1753806B1 (en) * 2004-05-05 2008-07-30 Firmenich Sa Biodegradable grafted copolymers
WO2005118877A2 (en) 2004-06-02 2005-12-15 Vicus Bioscience, Llc Producing, cataloging and classifying sequence tags
US7632924B2 (en) 2004-06-18 2009-12-15 Ambrx, Inc. Antigen-binding polypeptides and their uses
EP2359842A1 (en) 2004-07-14 2011-08-24 University of Utah Research Foundation Netrin-related compositions and uses
CA2910494C (en) 2004-07-19 2018-10-23 Biocon Limited Insulin-oligomer conjugates, formulations and uses thereof
JP5090915B2 (en) * 2004-10-08 2012-12-05 フイルメニツヒ ソシエテ アノニム Amphiphilic star block polymer
US9492400B2 (en) 2004-11-04 2016-11-15 Massachusetts Institute Of Technology Coated controlled release polymer particles as efficient oral delivery vehicles for biopharmaceuticals
US7446157B2 (en) 2004-12-07 2008-11-04 Key Medical Technologies, Inc. Nanohybrid polymers for ophthalmic applications
US20120269886A1 (en) 2004-12-22 2012-10-25 Nitto Denko Corporation Therapeutic agent for pulmonary fibrosis
CN101087875B (en) 2004-12-22 2012-08-22 Ambrx公司 Compositions of aminoacyl-tRNA synthetase and uses thereof
PT2727583T (en) 2004-12-22 2021-12-27 Nitto Denko Corp Drug carrier and drug carrier kit for inhibiting fibrosis
NZ555386A (en) 2004-12-22 2011-01-28 Ambrx Inc Formulations of human growth hormone comprising a non-naturally encoded amino acid
US8080391B2 (en) 2004-12-22 2011-12-20 Ambrx, Inc. Process of producing non-naturally encoded amino acid containing high conjugated to a water soluble polymer
SG160437A1 (en) 2004-12-22 2010-04-29 Ambrx Inc Modified human growth hormone
WO2006110862A2 (en) 2005-04-12 2006-10-19 Wisconsin Alumni Research Foundation Micelle composition of polymer and passenger drug
US20060263328A1 (en) * 2005-05-19 2006-11-23 Sang Van Hydrophilic polymers with pendant functional groups and method thereof
KR100664558B1 (en) 2005-05-23 2007-01-03 박기동 Amphiphilic copolymer having multi functional group and preparation method thereof
CN101247821B (en) 2005-06-03 2013-01-23 Ambrx公司 Improved human interferon molecules and their uses
WO2006130978A1 (en) 2005-06-06 2006-12-14 The University Of British Columbia Polymer-based serum albumin substitute
EP1898955A2 (en) * 2005-06-17 2008-03-19 Nektar Therapeutics AL, Corporation Polymer-based compositions and conjugates of non-steroidal anti-inflammatory drugs
US20070009564A1 (en) * 2005-06-22 2007-01-11 Mcclain James B Drug/polymer composite materials and methods of making the same
KR100642220B1 (en) * 2005-07-12 2006-11-03 광주과학기술원 Conjugates of cyclodextrin and poly(oxyethylene) and process for preparation thereof
WO2007011708A2 (en) 2005-07-15 2007-01-25 Micell Technologies, Inc. Stent with polymer coating containing amorphous rapamycin
AU2006270221B2 (en) 2005-07-15 2012-01-19 Micell Technologies, Inc. Polymer coatings containing drug powder of controlled morphology
EP1926768B1 (en) 2005-07-18 2011-09-14 Nektar Therapeutics Branched functionalized polymers using branched polyol cores
US20080184618A1 (en) * 2005-08-03 2008-08-07 Amcol International Virus-Interacting Layered Phyllosilicates and Methods of Use
US20100272769A1 (en) * 2005-08-03 2010-10-28 Amcol International Virus-, Bacteria-, and Fungi-Interacting Layered Phyllosilicates and Methods of Use
US20070031512A1 (en) * 2005-08-03 2007-02-08 Amcol International Corporation Virus-interacting layered phyllosilicates and methods of inactivating viruses
DE102005051342A1 (en) * 2005-10-25 2007-04-26 Goldschmidt Gmbh Encapsulation and controlled release of biologically active drugs with enzymatically degradable hyperbranched carrier polymers
JP5264492B2 (en) * 2005-10-25 2013-08-14 エボニック デグサ ゲーエムベーハー Preparations containing hyperbranched polymers
DK2339014T3 (en) 2005-11-16 2015-07-20 Ambrx Inc Methods and compositions comprising non-natural amino acids
WO2007070682A2 (en) 2005-12-15 2007-06-21 Massachusetts Institute Of Technology System for screening particles
US9572886B2 (en) 2005-12-22 2017-02-21 Nitto Denko Corporation Agent for treating myelofibrosis
ES2523431T3 (en) 2006-01-19 2014-11-25 Allexcel, Inc. Solubilization and targeted supply of drugs with self-assembly amphiphilic polymers
US9603941B2 (en) * 2006-01-24 2017-03-28 Minghui Chai Method of preparing dendritic drugs
KR101513732B1 (en) 2006-02-21 2015-04-21 넥타르 테라퓨틱스 Segmented degradable polymers and conjugates made therefrom
WO2007127363A2 (en) * 2006-04-26 2007-11-08 Micell Technologies, Inc. Coatings containing multiple drugs
JP5630998B2 (en) 2006-05-15 2014-11-26 マサチューセッツ インスティテュート オブ テクノロジー Polymers for functional particles
WO2007150030A2 (en) 2006-06-23 2007-12-27 Massachusetts Institute Of Technology Microfluidic synthesis of organic nanoparticles
WO2008005509A2 (en) * 2006-07-06 2008-01-10 Massachusetts Institute Of Technology Methods and compositions for altering biological surfaces
US20100022481A1 (en) * 2006-08-02 2010-01-28 Dong Wang Drug Carriers, Their Synthesis, and Methods of Use Thereof
EP2046391A4 (en) * 2006-08-02 2013-03-20 Univ Nebraska Drug carriers, their synthesis, and methods of use thereof
DE102006038240A1 (en) * 2006-08-07 2008-02-14 Biotronik Vi Patent Ag Process for the preparation of a composite of oligo- or polynucleotides and hydrophobic biodegradable polymers and composite obtained by the process
PT2061878E (en) 2006-09-08 2014-04-22 Ambrx Inc Hybrid suppressor trna for vertebrate cells
CN106008699A (en) * 2006-09-08 2016-10-12 Ambrx公司 Modified human plasma polypeptide or Fc scaffolds and their uses
CA2662755C (en) 2006-09-08 2017-04-04 Ambrx, Inc. Suppressor trna transcription in vertebrate cells
US8636767B2 (en) 2006-10-02 2014-01-28 Micell Technologies, Inc. Surgical sutures having increased strength
EP2081694B1 (en) * 2006-10-23 2020-05-13 Micell Technologies, Inc. Holder for electrically charging a substrate during coating
US20080153982A1 (en) * 2006-12-20 2008-06-26 John Ta-Yuan Lai Vinyl-Thiocarbonate and Polyol Containing Block Copolymers
JP5603598B2 (en) 2007-01-08 2014-10-08 ミセル テクノロジーズ、インコーポレイテッド Stent with biodegradable layer
US11426494B2 (en) 2007-01-08 2022-08-30 MT Acquisition Holdings LLC Stents having biodegradable layers
EP2134830A2 (en) 2007-02-09 2009-12-23 Massachusetts Institute of Technology Oscillating cell culture bioreactor
JP5052919B2 (en) * 2007-03-08 2012-10-17 成均▲館▼大學校 産學協力團 PH and temperature sensitive block copolymer hydrogel
ES2385114T3 (en) 2007-03-30 2012-07-18 Ambrx, Inc. Modified FGF-21 polypeptides and their uses
TWI407971B (en) 2007-03-30 2013-09-11 Nitto Denko Corp Cancer cells and tumor-related fibroblasts
JP2010523595A (en) 2007-04-04 2010-07-15 マサチューセッツ インスティテュート オブ テクノロジー Poly (amino acid) targeting part
US20100211164A1 (en) * 2007-04-17 2010-08-19 Mcclain James B Stents having biodegradable layers
EP1982698A1 (en) * 2007-04-18 2008-10-22 Evonik Degussa GmbH Preparations for controlled release of natural bioactive materials
US8114630B2 (en) 2007-05-02 2012-02-14 Ambrx, Inc. Modified interferon beta polypeptides and their uses
ATE530178T1 (en) 2007-05-23 2011-11-15 Amcol International Corp PHYLLOSSILICATES INTERACTING WITH CHOLESTEROL AND METHOD FOR REDUCING HYPERCHOLESTEROLEMIA IN A MAMMAL
EP2170418B1 (en) * 2007-05-25 2016-03-16 Micell Technologies, Inc. Polymer films for medical device coating
WO2008150845A1 (en) * 2007-05-31 2008-12-11 Vanderbilt University Screening for wnt pathway modulators and pyrvinium for the treatment of cance
CA2696996C (en) 2007-07-16 2016-03-22 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon State University Antimicrobial constructs
US20110130822A1 (en) * 2007-07-20 2011-06-02 Orbusneich Medical, Inc. Bioabsorbable Polymeric Compositions and Medical Devices
WO2009036368A2 (en) 2007-09-14 2009-03-19 Nitto Denko Corporation Drug carriers
WO2009042114A2 (en) 2007-09-21 2009-04-02 The Johns Hopkins University Phenazine derivatives and uses thereof
WO2009051837A2 (en) 2007-10-12 2009-04-23 Massachusetts Institute Of Technology Vaccine nanotechnology
WO2009051780A1 (en) * 2007-10-19 2009-04-23 Micell Technologies, Inc. Drug coated stents
CN101835496B (en) * 2007-10-23 2015-11-25 尼克塔治疗公司 The multiarm polymers of hydroxyapatite-targeting and the conjugate by its manufacture
JP5547083B2 (en) 2007-11-20 2014-07-09 アンブルックス,インコーポレイテッド Modified insulin polypeptides and their use
US9801818B2 (en) 2007-12-31 2017-10-31 Samyang Biopharmaceuticals Corporation Method for stabilizing amphiphilic block copolymer micelle composition containing poorly water-soluble drug
KR101024742B1 (en) * 2007-12-31 2011-03-24 주식회사 삼양사 Amphiphilic Block Copolymer Micelle Composition Containing Taxane and Manufacturing Process of The Same
EP2247743B1 (en) 2008-02-08 2016-04-06 Ambrx, Inc. Modified leptin polypeptides and their uses
TWI395593B (en) 2008-03-06 2013-05-11 Halozyme Inc In vivo temporal control of activatable matrix-degrading enzymes
NZ601248A (en) 2008-04-14 2014-06-27 Halozyme Inc Modified hyaluronidases and uses in treating hyaluronan-associated diseases and conditions
SG192523A1 (en) * 2008-04-17 2013-08-30 Micell Technologies Inc Stents having bioabsorbable layers
TWI394580B (en) 2008-04-28 2013-05-01 Halozyme Inc Super fast-acting insulin compositions
CA2730085A1 (en) * 2008-07-09 2010-01-14 Board Of Regents Of The University Of Nebraska Functional micelles for hard tissue targeted delivery of chemicals
CN102159257B (en) 2008-07-17 2015-11-25 米歇尔技术公司 Drug delivery medical device
MX2011000859A (en) 2008-07-23 2011-02-24 Ambrx Inc Modified bovine g-csf polypeptides and their uses.
US9991391B2 (en) * 2008-07-25 2018-06-05 The Board Of Trustees Of The Leland Stanford Junior University Pristine and functionalized graphene materials
CA2736939C (en) 2008-09-23 2021-09-14 Nektar Therapeutics Means of achieving sustained-therapeutic sn-38 concentrations in a subject
CA2737026C (en) 2008-09-26 2019-12-24 Ambrx, Inc. Modified animal erythropoietin polypeptides and their uses
PL2337846T3 (en) 2008-09-26 2018-06-29 Ambrx, Inc. Non-natural amino acid replication-dependent microorganisms and vaccines
US8277812B2 (en) 2008-10-12 2012-10-02 Massachusetts Institute Of Technology Immunonanotherapeutics that provide IgG humoral response without T-cell antigen
US8343497B2 (en) 2008-10-12 2013-01-01 The Brigham And Women's Hospital, Inc. Targeting of antigen presenting cells with immunonanotherapeutics
US8343498B2 (en) 2008-10-12 2013-01-01 Massachusetts Institute Of Technology Adjuvant incorporation in immunonanotherapeutics
US8591905B2 (en) 2008-10-12 2013-11-26 The Brigham And Women's Hospital, Inc. Nicotine immunonanotherapeutics
CN101724144A (en) 2008-11-03 2010-06-09 北京键凯科技有限公司 Novel multi-arm polyethylene glycol, preparation method and application thereof
SI3037529T1 (en) 2008-12-09 2019-06-28 Halozyme, Inc. Extended soluble ph20 polypeptides and uses thereof
US8834913B2 (en) 2008-12-26 2014-09-16 Battelle Memorial Institute Medical implants and methods of making medical implants
US20100239635A1 (en) 2009-03-23 2010-09-23 Micell Technologies, Inc. Drug delivery medical device
EP2411440B1 (en) * 2009-03-23 2018-01-17 Micell Technologies, Inc. Improved biodegradable polymers
US20100241220A1 (en) * 2009-03-23 2010-09-23 Mcclain James B Peripheral Stents Having Layers
CA2757276C (en) * 2009-04-01 2017-06-06 Micell Technologies, Inc. Coated stents
CA2759015C (en) 2009-04-17 2017-06-20 James B. Mcclain Stents having controlled elution
CN101869712B (en) * 2009-04-21 2016-01-20 财团法人工业技术研究院 The polymeric micelle of encapsulation antitumor drug is used to be used for the treatment of the pharmaceutical composition of tumor
US9572693B2 (en) 2009-05-14 2017-02-21 Orbusneich Medical, Inc. Self-expanding stent with polygon transition zone
US8013065B2 (en) * 2009-06-09 2011-09-06 International Business Machines Corporation Methods for making multi-branched polymers
EP2453834A4 (en) 2009-07-16 2014-04-16 Micell Technologies Inc Drug delivery medical device
ES2578478T3 (en) 2009-09-17 2016-07-27 Baxalta Incorporated Stable co-formulation of hyaluronidase and immunoglobulin, and methods of use thereof
US20110070320A1 (en) * 2009-09-23 2011-03-24 Soonkap Hahn Biodegradable thermoresponsive 3-arm polyethylene glycol poly(lactide-co-glycolide) copolymer for ginseng administration
PE20121799A1 (en) 2009-12-21 2013-01-02 Ambrx Inc MODIFIED PORCINE SOMATOTROPIN POLYPEPTIDES AND THEIR USES
EA201290541A1 (en) 2009-12-21 2013-05-30 Амбркс, Инк. MODIFIED BULL SOMATOTROPINE POLYPEPTIDES AND THEIR APPLICATION
EP2531140B1 (en) 2010-02-02 2017-11-01 Micell Technologies, Inc. Stent and stent delivery system with improved deliverability
CN102906157B (en) 2010-03-01 2016-08-24 不列颠哥伦比亚大学 Derivative hyperbranched poly glycerine
US8795762B2 (en) 2010-03-26 2014-08-05 Battelle Memorial Institute System and method for enhanced electrostatic deposition and surface coatings
US8765098B2 (en) 2010-03-30 2014-07-01 International Business Machines Corporation Star polymers, methods of preparation thereof, and uses thereof
CA2797110C (en) 2010-04-22 2020-07-21 Micell Technologies, Inc. Stents and other devices having extracellular matrix coating
CN103119055A (en) 2010-05-17 2013-05-22 塞比克斯公司 Pegylated C-Peptide
EP2585533B1 (en) 2010-06-25 2017-05-17 E. I. du Pont de Nemours and Company Polyoxymethylene compositions with branched polymers
WO2012009684A2 (en) 2010-07-16 2012-01-19 Micell Technologies, Inc. Drug delivery medical device
WO2012012300A2 (en) 2010-07-20 2012-01-26 Halozyme, Inc. Adverse side-effects associated with administration of an anti-hyaluronan agent and methods for ameliorating or preventing the side-effects
US9567386B2 (en) 2010-08-17 2017-02-14 Ambrx, Inc. Therapeutic uses of modified relaxin polypeptides
AU2011291943B2 (en) 2010-08-17 2015-01-22 Ambrx, Inc. Modified relaxin polypeptides and their uses
TWI480288B (en) 2010-09-23 2015-04-11 Lilly Co Eli Formulations for bovine granulocyte colony stimulating factor and variants thereof
WO2012070030A1 (en) * 2010-11-26 2012-05-31 University Of The Witwatersrand, Johannesburg A pharmaceutical composition
CA2823355C (en) 2010-12-30 2017-08-22 Micell Technologies, Inc. Nanoparticle and surface-modified particulate coatings, coated balloons, and methods therefore
ES2634669T3 (en) 2011-02-08 2017-09-28 Halozyme, Inc. Composition and lipid formulation of a hyaluronan degradation enzyme and use thereof for the treatment of benign prostatic hyperplasia
US20120323311A1 (en) 2011-04-13 2012-12-20 Micell Technologies, Inc. Stents having controlled elution
US10464100B2 (en) 2011-05-31 2019-11-05 Micell Technologies, Inc. System and process for formation of a time-released, drug-eluting transferable coating
KR101676543B1 (en) 2011-06-17 2016-11-15 할로자임, 아이엔씨 Continuous Subcutaneous Insulin Infusion Methods with a Hyaluronan Degrading Enzyme
US9993529B2 (en) 2011-06-17 2018-06-12 Halozyme, Inc. Stable formulations of a hyaluronan-degrading enzyme
CA2841360A1 (en) 2011-07-15 2013-01-24 Micell Technologies, Inc. Drug delivery medical device
US8445577B2 (en) * 2011-07-25 2013-05-21 Iowa State University Research Foundation, Inc. Amphiphilic multi-arm copolymers and nanomaterials derived therefrom
WO2013040501A1 (en) 2011-09-16 2013-03-21 Pharmathene, Inc. Compositions and combinations of organophosphorus bioscavengers and hyaluronan-degrading enzymes, and uses thereof
US10188772B2 (en) 2011-10-18 2019-01-29 Micell Technologies, Inc. Drug delivery medical device
CA2853358A1 (en) 2011-10-24 2013-05-02 Halozyme, Inc. Companion diagnostic for anti-hyaluronan agent therapy and methods of use thereof
SI3130347T1 (en) 2011-12-30 2020-02-28 Halozyme, Inc. Ph20 polypeptide variants, formulations and uses thereof
ES2661575T3 (en) 2012-03-30 2018-04-02 The Board Of Regents Of The University Of Oklahoma Production of high molecular weight heparosan polymers and methods of production and use thereof
SI2833905T1 (en) 2012-04-04 2018-08-31 Halozyme, Inc. Combination therapy with hyaluronidase and a tumor-targeted taxane
WO2013176739A1 (en) * 2012-05-25 2013-11-28 Regents Of The University Of Minnesota Biomaterials with a sustainable surface
EP2859017B1 (en) 2012-06-08 2019-02-20 Sutro Biopharma, Inc. Antibodies comprising site-specific non-natural amino acid residues, methods of their preparation and methods of their use
EP3135690A1 (en) 2012-06-26 2017-03-01 Sutro Biopharma, Inc. Modified fc proteins comprising site-specific non-natural amino acid residues, conjugates of the same, methods of their preparation and methods of their use
EP3584255B1 (en) 2012-08-31 2022-02-16 Sutro Biopharma, Inc. Modified amino acids comprising an azido group
CN102861610B (en) * 2012-09-03 2014-05-07 宁波长阳科技有限公司 Hyperbranched amido pyridine contained catalyst, preparation methods of catalyst and application of catalyst
WO2014062856A1 (en) 2012-10-16 2014-04-24 Halozyme, Inc. Hypoxia and hyaluronan and markers thereof for diagnosis and monitoring of diseases and conditions and related methods
CN103910874A (en) * 2013-01-06 2014-07-09 张雅珍 Preparation and application of medicine-grafted polymer
CN104193987A (en) * 2013-01-21 2014-12-10 张雅珍 Preparation and uses of grafted medicine polymers
WO2014165264A1 (en) 2013-03-12 2014-10-09 Micell Technologies, Inc. Bioabsorbable biomedical implants
WO2014172261A1 (en) 2013-04-15 2014-10-23 Kent State University Biodegradable side chain liquid crystal elastomers: smart responsive scaffolds (srs) for tissue regeneration
EP2996629B1 (en) 2013-05-15 2021-09-22 Micell Technologies, Inc. Bioabsorbable biomedical implants
TW201534726A (en) 2013-07-03 2015-09-16 Halozyme Inc Thermally stable PH20 hyaluronidase variants and uses thereof
ES2865473T3 (en) 2013-07-10 2021-10-15 Sutro Biopharma Inc Antibodies Comprising Multiple Site-Specific Unnatural Amino Acid Residues, Methods for Their Preparation, and Methods of Use
CN104910373A (en) * 2013-08-25 2015-09-16 张雅珍 New polymer, preparation and application thereof
ES2650796T3 (en) 2013-09-09 2018-01-22 Gambro Lundia Ab Separation of interference pulses from physiological pulses in a pressure signal
WO2015038966A1 (en) * 2013-09-12 2015-03-19 Regents Of The University Of Minnesota Semi-solid delivery systems
US9433638B1 (en) 2013-10-02 2016-09-06 University Of Kentucky Research Foundation Polymeric prodrug
US9840493B2 (en) 2013-10-11 2017-12-12 Sutro Biopharma, Inc. Modified amino acids comprising tetrazine functional groups, methods of preparation, and methods of their use
CA2954064C (en) 2014-07-02 2018-10-30 The Research Foundation For The State University Of New York Surfactant-stripped micelle compositions with high cargo to surfactant ratio
WO2016033555A1 (en) 2014-08-28 2016-03-03 Halozyme, Inc. Combination therapy with a hyaluronan-degrading enzyme and an immune checkpoint inhibitor
ES2753391T3 (en) 2014-10-14 2020-04-08 Halozyme Inc Adenosine deaminase 2 (ADA2) compositions, variants thereof and methods of use thereof
PE20170950A1 (en) 2014-10-24 2017-07-13 Bristol Myers Squibb Co MODIFIED FIBROBLAST GROWTH FACTOR 2 (FGF-21) POLYEPTIDES AND USES OF THE SAME
CN105395483B (en) * 2015-12-21 2018-09-28 江南大学 One kind can deoxidization, degradation dissaving polymer nano-micelle and preparation method thereof
US10792477B2 (en) 2016-02-08 2020-10-06 Orbusneich Medical Pte. Ltd. Drug eluting balloon
US20210196932A1 (en) 2016-02-08 2021-07-01 Orbusneich Medical, Inc. Drug Eluting Balloon
CN105778021B (en) 2016-04-05 2018-07-10 华南理工大学 Beta-cyclodextrin base star polymer and preparation method and its unimolecular micelle diagnosis and treatment integral system
JP7032406B2 (en) * 2016-09-13 2022-03-08 ラスナ・リサーチ・インコーポレイテッド Dactinomycin composition and method for treating myelodysplastic syndrome and acute myeloid leukemia
US20180085498A1 (en) 2016-09-23 2018-03-29 Micell Technologies, Inc. Prolonged drug-eluting products
US11027040B2 (en) * 2016-12-29 2021-06-08 Industrial Technology Research Institute Method for manufacturing a porous film, porous film and method for tissue adhesion
ES2963839T3 (en) 2017-02-08 2024-04-02 Bristol Myers Squibb Co Modified relaxin polypeptides comprising a pharmacokinetic enhancer and uses thereof
BR112019027583A2 (en) 2017-06-22 2020-10-06 Catalyst Biosciences, Inc. modified membrane-like serine protease 1 (mtsp-1) polypeptides and methods of use
US10457759B2 (en) 2017-07-20 2019-10-29 International Business Machines Corporation Co-delivery of cholesterol lowering drugs and nutraceuticals
CN111526891B (en) * 2017-12-27 2023-10-20 三洋化成工业株式会社 Pharmaceutical raw material or pharmaceutical additive and pharmaceutical raw material or pharmaceutical using the same
CN108707212B (en) * 2018-05-02 2020-01-07 上海交通大学 Controllable preparation method of water-soluble conjugated polymer nanoparticles
US20190351031A1 (en) 2018-05-16 2019-11-21 Halozyme, Inc. Methods of selecting subjects for combination cancer therapy with a polymer-conjugated soluble ph20
US20220056093A1 (en) 2018-09-11 2022-02-24 Ambrx, Inc. Interleukin-2 polypeptide conjugates and their uses
CN113366015A (en) 2018-10-19 2021-09-07 Ambrx公司 Interleukin-10 polypeptide conjugates, dimers thereof and uses thereof
CN109608633A (en) * 2018-11-09 2019-04-12 北京诺康达医药科技股份有限公司 A kind of novel specific multi-arm polyethylene glycol derivative and preparation method thereof
US11613744B2 (en) 2018-12-28 2023-03-28 Vertex Pharmaceuticals Incorporated Modified urokinase-type plasminogen activator polypeptides and methods of use
JP2022516725A (en) 2018-12-28 2022-03-02 カタリスト・バイオサイエンシーズ・インコーポレイテッド Modified urokinase-type plasminogen activator polypeptide and usage
EP3680273B1 (en) * 2019-01-08 2022-03-09 Université de Montpellier Branched-block copolymer photo-crosslinker functionalized with photoreactive groups and its use for shaping degradable photo-crosslinked elastomers suitable for medical and tissue-engineering applications
CA3128081A1 (en) 2019-02-12 2020-08-20 Ambrx, Inc. Compositions containing, methods and uses of antibody-tlr agonist conjugates
CN109929083B (en) 2019-04-10 2020-04-28 浙江大学 Narrow molecular weight distribution multi-block copolymer and preparation method thereof
CN110078901A (en) * 2019-05-24 2019-08-02 上海典范医疗科技有限公司 A kind of poly-dl-lactide preparation method
EP3862032A1 (en) 2020-02-07 2021-08-11 Micell Technologies, Inc. Stents having biodegradable layers
EP4117732A1 (en) 2020-03-11 2023-01-18 Ambrx, Inc. Interleukin-2 polypeptide conjugates and methods of use thereof
CN111759552A (en) 2020-07-06 2020-10-13 苏州莱诺医疗器械有限公司 Absorbable stent system
AU2021327396A1 (en) 2020-08-20 2023-03-23 Ambrx, Inc. Antibody-TLR agonist conjugates, methods and uses thereof
EP4313163A1 (en) 2021-04-03 2024-02-07 Ambrx, Inc. Anti-her2 antibody-drug conjugates and uses thereof
CN113416302B (en) * 2021-07-08 2022-06-17 中国科学院兰州化学物理研究所 Mercaptan-acrylate biomaterial and preparation method and application thereof
CN114713206B (en) * 2022-03-15 2023-11-14 沈阳工业大学 Preparation and application of imidazole ionic liquid functionalized star-type block polymer stationary phase

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US673034A (en) * 1900-08-07 1901-04-30 Russel B Sigafoos Trussed ladder.
US3101374A (en) * 1958-08-19 1963-08-20 Wyandotte Chemicals Corp Polyoxyalkylene surface active agents having heteric polyoxyethylene solubilizing chains
US4278555A (en) 1978-11-15 1981-07-14 Ethyl Corporation Lubricant composition
ZA801659B (en) 1979-03-21 1981-10-28 N Graham Controlled release compositions
JPS59155432A (en) 1983-02-22 1984-09-04 Rikagaku Kenkyusho Surface-modified polymer having molecular chain of large mobility
US6436425B1 (en) 1988-11-16 2002-08-20 Mdv Technologies, Inc. Method and non-gelling composition for inhibiting post-surgical adhesions
US5681576A (en) 1988-11-16 1997-10-28 Mdv Technologies, Inc. Method and composition for post surgical adhesion reduction
US5135751A (en) 1988-11-16 1992-08-04 Mediventures Incorporated Composition for reducing postsurgical adhesions
US5306501A (en) 1990-05-01 1994-04-26 Mediventures, Inc. Drug delivery by injection with thermoreversible gels containing polyoxyalkylene copolymers
DE4017688A1 (en) 1990-06-01 1991-12-05 Bayer Ag IMPROVED FUNCTIONAL LIQUIDS AND NEW POLYOXYALKYLENE POLYMERS
WO1992000748A1 (en) 1990-07-06 1992-01-23 Enzon, Inc. Poly(alkylene oxide) amino acid copolymers and drug carriers and charged copolymers based thereon
IE912365A1 (en) 1990-07-23 1992-01-29 Zeneca Ltd Continuous release pharmaceutical compositions
CA2087125A1 (en) 1992-01-23 1993-07-24 Mridula Nair Chemically fixed micelles
US6096331A (en) 1993-02-22 2000-08-01 Vivorx Pharmaceuticals, Inc. Methods and compositions useful for administration of chemotherapeutic agents
US5543158A (en) * 1993-07-23 1996-08-06 Massachusetts Institute Of Technology Biodegradable injectable nanoparticles
US5965566A (en) 1993-10-20 1999-10-12 Enzon, Inc. High molecular weight polymer-based prodrugs
US5618528A (en) 1994-02-28 1997-04-08 Sterling Winthrop Inc. Biologically compatible linear block copolymers of polyalkylene oxide and peptide units
US5626862A (en) 1994-08-02 1997-05-06 Massachusetts Institute Of Technology Controlled local delivery of chemotherapeutic agents for treating solid tumors
US5932462A (en) 1995-01-10 1999-08-03 Shearwater Polymers, Inc. Multiarmed, monofunctional, polymer for coupling to molecules and surfaces
US5672662A (en) 1995-07-07 1997-09-30 Shearwater Polymers, Inc. Poly(ethylene glycol) and related polymers monosubstituted with propionic or butanoic acids and functional derivatives thereof for biotechnical applications
ATE330644T1 (en) 1995-12-18 2006-07-15 Angiotech Biomaterials Corp CROSS-LINKED POLYMER MATERIALS AND METHODS FOR USE THEREOF
US5610241A (en) 1996-05-07 1997-03-11 Cornell Research Foundation, Inc. Reactive graft polymer with biodegradable polymer backbone and method for preparing reactive biodegradable polymers
US6214966B1 (en) 1996-09-26 2001-04-10 Shearwater Corporation Soluble, degradable poly(ethylene glycol) derivatives for controllable release of bound molecules into solution
US6258351B1 (en) 1996-11-06 2001-07-10 Shearwater Corporation Delivery of poly(ethylene glycol)-modified molecules from degradable hydrogels
US6011042A (en) 1997-10-10 2000-01-04 Enzon, Inc. Acyl polymeric derivatives of aromatic hydroxyl-containing compounds
ZA9811376B (en) 1997-12-12 1999-06-28 Expression Genetics Inc Biodegradable mixed polymeric micelles for gene delivery
ES2211033T3 (en) 1998-01-07 2004-07-01 Debio Recherche Pharmaceutique S.A. DEGRADABLE HETEROBIFUNCTIONAL POLYETHYLENGLYCOL ACRYLATES AND GELS AND CONJUGATES DERIVED FROM SUCH ACRYLATES
EP1061954B1 (en) * 1998-03-12 2004-06-09 Nektar Therapeutics Al, Corporation Poly(ethylene glycol) derivatives with proximal reactive groups
US20030059465A1 (en) 1998-05-11 2003-03-27 Unger Evan C. Stabilized nanoparticle formulations of camptotheca derivatives
US6328988B1 (en) * 1999-04-23 2001-12-11 Rutgers, The State University Of New Jersey Hyperbranched polymeric micelles for encapsulation and delivery of hydrophobic molecules
US6348558B1 (en) 1999-12-10 2002-02-19 Shearwater Corporation Hydrolytically degradable polymers and hydrogels made therefrom
US20040009229A1 (en) 2000-01-05 2004-01-15 Unger Evan Charles Stabilized nanoparticle formulations of camptotheca derivatives
JP2003520210A (en) 2000-01-05 2003-07-02 イマレックス セラピューティクス, インコーポレイテッド Pharmaceutical formulations for delivery of drugs with low water solubility
US20020041898A1 (en) 2000-01-05 2002-04-11 Unger Evan C. Novel targeted delivery systems for bioactive agents
FI109162B (en) * 2000-06-30 2002-05-31 Nokia Corp Method and arrangement for decoding a convolution-coded codeword
US7265186B2 (en) * 2001-01-19 2007-09-04 Nektar Therapeutics Al, Corporation Multi-arm block copolymers as drug delivery vehicles
TWI246524B (en) 2001-01-19 2006-01-01 Shearwater Corp Multi-arm block copolymers as drug delivery vehicles

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