CA2437962A1 - Method of extracting virus from cell culture - Google Patents

Method of extracting virus from cell culture Download PDF

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Publication number
CA2437962A1
CA2437962A1 CA002437962A CA2437962A CA2437962A1 CA 2437962 A1 CA2437962 A1 CA 2437962A1 CA 002437962 A CA002437962 A CA 002437962A CA 2437962 A CA2437962 A CA 2437962A CA 2437962 A1 CA2437962 A1 CA 2437962A1
Authority
CA
Canada
Prior art keywords
cell culture
virus
extracting virus
extracting
culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002437962A
Other languages
French (fr)
Other versions
CA2437962C (en
Inventor
Matthew C. Coffey
Bradley G. Thompson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oncolytics Biotech Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2437962A1 publication Critical patent/CA2437962A1/en
Application granted granted Critical
Publication of CA2437962C publication Critical patent/CA2437962C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/76—Viruses; Subviral particles; Bacteriophages
    • A61K35/765—Reovirus; Rotavirus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/12011—Reoviridae
    • C12N2720/12211—Orthoreovirus, e.g. mammalian orthoreovirus
    • C12N2720/12232—Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/12011—Reoviridae
    • C12N2720/12211—Orthoreovirus, e.g. mammalian orthoreovirus
    • C12N2720/12251—Methods of production or purification of viral material

Abstract

The present invention is directed to a method of extracting virus, particularly reovirus, from a culture of cells. Infectious virus can be extracted from the culture with a detergent at a convenient temperature such as 25 ~C or 37 ~C to produce high virus titers. Both ionic and non-ionic detergents can be used in the present invention.
CA002437962A 2001-03-16 2002-03-11 Method of extracting virus from cell culture Expired - Lifetime CA2437962C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US27673401P 2001-03-16 2001-03-16
US60/276,734 2001-03-16
PCT/CA2002/000331 WO2002074940A1 (en) 2001-03-16 2002-03-11 Method of extracting virus from cell culture

Publications (2)

Publication Number Publication Date
CA2437962A1 true CA2437962A1 (en) 2002-09-26
CA2437962C CA2437962C (en) 2005-11-15

Family

ID=29420186

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002437962A Expired - Lifetime CA2437962C (en) 2001-03-16 2002-03-11 Method of extracting virus from cell culture

Country Status (14)

Country Link
US (2) US6808916B2 (en)
EP (2) EP1370643A1 (en)
JP (2) JP4087712B2 (en)
AR (1) AR034216A1 (en)
BR (1) BR0207527A (en)
CA (1) CA2437962C (en)
DK (1) DK2253701T3 (en)
ES (1) ES2500645T3 (en)
HK (1) HK1150859A1 (en)
IL (2) IL157304A0 (en)
MX (1) MXPA03007535A (en)
NZ (1) NZ527525A (en)
WO (1) WO2002074940A1 (en)
ZA (1) ZA200306228B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006042414A1 (en) * 2004-10-22 2006-04-27 Oncolytics Biotech Inc. Improved viral purification methods
US8685734B2 (en) 2002-04-30 2014-04-01 Oncolytics Biotech Inc. Viral purification methods

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4389870B2 (en) * 2003-06-10 2009-12-24 株式会社島津製作所 Cell-free protein synthesis method using mammalian cultured cell extract
US10668119B2 (en) 2005-08-01 2020-06-02 Virocure, Inc. Attenuated reovirus
WO2007099401A2 (en) 2005-08-01 2007-09-07 University Technologies International, Inc. Oncolytic attenuated reoviruses for treatment of proliferative disorders
KR101647843B1 (en) 2007-03-12 2016-08-11 온콜리틱스 바이오테크 인코포레이티드 Reoviruses having modified sequences
US10369171B2 (en) 2007-03-13 2019-08-06 Virocure, Inc. Attenuated reoviruses for selection of cell populations
TW200909581A (en) * 2007-05-21 2009-03-01 Oncolytics Biotech Inc Mutant reoviruses and methods of making and using
AU2008316276A1 (en) * 2007-10-22 2009-04-30 Oncolytics Biotech Inc. Treatment regime for proliferative disorders
CN201163385Y (en) * 2008-01-15 2008-12-10 昆山渝榕电子有限公司 Notebook computer and its foot mat
TW200951143A (en) * 2008-05-27 2009-12-16 Oncolytics Biotech Inc Modulating interstitial pressure and oncolytic viral delivery and distribution
EP2296679A4 (en) * 2008-05-27 2012-03-21 Oncolytics Biotech Inc Abrogating proinflammatory cytokine production during oncolytic reovirus therapy
SG190419A1 (en) 2010-12-02 2013-06-28 Oncolytics Biotech Inc Lyophilized viral formulations
AU2011336413B2 (en) 2010-12-02 2015-01-22 Oncolytics Biotech Inc. Liquid viral formulations
MX337348B (en) 2011-04-29 2016-02-29 Oncolytics Biotech Inc Methods of purifying viruses using gel permeation chromatography.
CN105247036B (en) * 2013-03-15 2019-04-02 安迅生物制药公司 The novel method of protein purification
WO2015070323A1 (en) 2013-11-15 2015-05-21 Oncolytics Biotech Inc. Oncolytic viruses and increased cancer treatment regimens
US10280199B2 (en) 2014-02-07 2019-05-07 Phibro Animal Health Corporation Coronavirus proteins and antigens
WO2016130569A1 (en) * 2015-02-09 2016-08-18 Mj Biologics, Inc. A composition comprising pedv antigens and methods for making and using the composition
US10294460B2 (en) 2014-07-24 2019-05-21 Janssen Vaccines & Prevention B.V. Process for the purification of poliovirus from cell cultures
GB201417042D0 (en) * 2014-09-29 2014-11-12 Fkd Therapies Oy Method
JPWO2023277173A1 (en) * 2021-06-30 2023-01-05

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5023252A (en) 1985-12-04 1991-06-11 Conrex Pharmaceutical Corporation Transdermal and trans-membrane delivery of drugs
JPS6344532A (en) 1986-08-11 1988-02-25 Biseibutsu Kagaku Kenkyusho:Kk Vaccine for reovirus infection disease or swine
US5614413A (en) * 1993-07-01 1997-03-25 The Uab Research Foundation Encapsidated recombinant poliovirus nucleic acid and methods of making and using same
HRP950097A2 (en) * 1994-03-08 1997-06-30 Merck & Co Inc Hepatitis a virus culture process
US6146873A (en) * 1994-11-10 2000-11-14 Baxter Aktiengesellschaft Production of orthomyxoviruses in monkey kidney cells using protein-free media
CN1244215B (en) * 1996-11-20 2010-11-03 荷兰克鲁塞尔公司 Improved method for the production and purification of adenoviral vectors
US6214333B1 (en) * 1997-07-08 2001-04-10 Texas Heart Institute Vasoprotective recombinant adenovirus vector containing a human TFPI gene
US6110461A (en) 1997-08-13 2000-08-29 Oncolytics Biotech Inc. Reovirus for the treatment of neoplasia
TWI289158B (en) 2000-08-10 2007-11-01 Oncolytics Biotech Inc Method of producing infectious reovirus

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8685734B2 (en) 2002-04-30 2014-04-01 Oncolytics Biotech Inc. Viral purification methods
US10167452B2 (en) 2002-04-30 2019-01-01 Oncolytics Biotech Inc. Viral purification methods
WO2006042414A1 (en) * 2004-10-22 2006-04-27 Oncolytics Biotech Inc. Improved viral purification methods
AU2005297372B2 (en) * 2004-10-22 2010-08-26 Oncolytics Biotech Inc. Improved viral purification methods
US7901921B2 (en) 2004-10-22 2011-03-08 Oncolytics Biotech Inc. Viral purification methods

Also Published As

Publication number Publication date
HK1150859A1 (en) 2012-01-13
JP4087712B2 (en) 2008-05-21
IL157304A (en) 2010-04-29
ZA200306228B (en) 2004-08-12
NZ527525A (en) 2005-03-24
US20020168764A1 (en) 2002-11-14
EP2253701B1 (en) 2014-08-13
AR034216A1 (en) 2004-02-04
US20050095692A1 (en) 2005-05-05
EP1370643A1 (en) 2003-12-17
MXPA03007535A (en) 2003-12-11
DK2253701T3 (en) 2014-10-20
US6808916B2 (en) 2004-10-26
IL157304A0 (en) 2004-02-19
US7186542B2 (en) 2007-03-06
WO2002074940A1 (en) 2002-09-26
EP2253701A1 (en) 2010-11-24
JP2007295937A (en) 2007-11-15
CA2437962C (en) 2005-11-15
BR0207527A (en) 2004-02-25
JP2004520841A (en) 2004-07-15
ES2500645T3 (en) 2014-09-30

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Effective date: 20220311