CA2443968A1 - Diagnosis and treatment of cancer: i - Google Patents

Abstract

A method of diagnosing cancer comprising the steps of (i) obtaining a sample containing nucleic acid and/or protein from the patient; and (ii) determining whether the sample contains a level of SCN5A (and optionally also SCN9A) voltage-gated Na+ channel nucleic acid or protein associated with cancer. A method of diagnosing breast cancer comprising the steps of (i) obtaining a sample containing nucleic acid and/or protein from the patient; and (ii) determining whether the sample contains a level of voltage-gated Na+ channel nucleic acid or protein, preferably SCN5A or SCN9A, associated with cancer. A method of treating cancer comprising the step of administering to the patient an agent which selectively prevents the function of SCN5A (and optionally also SCN9A) voltage-gated Na+ channel. A method of treating breast cancer comprising the step of administering to the patient an agent which selectively prevents the function of a voltage-gated Na+ channel, preferably SCN5A or SCN9A. Genetic constructs and molecules useful in such methods. The methods and compositions are particularly suited to breast cancer.

Description

DIAGNOSIS AND TREATMENT OF CANCER: I
The present invention relates to methods of determining whether a patient has cancer and whether the cancer is likely to metastasise; and it relates to s methods of treating cancer, particularly breast cancer.
Cancer is a serious disease and a major killer. Although there have been advances in the diagnosis and treatment of certain cancers in recent years, there is still a need for improvements in diagnosis and treatment.
to Cancer is a genetic disease and in most cases involves mutations in one or more genes. There are believed to be around 40,000 genes in the human genome but only a handful of these genes have been shown to be involved in cancer. Although it is surmised that many more genes than have been presently identified will be found to be involved in cancer, progress in this area has remained slow despite the availability of molecular analytical techniques. This may be due to the varied structure and function of genes which have been identified to date which suggests that cancer genes can take many forms, occur in different combinations and have many different 2o functions.
Breast cancer is one of the most significant diseases that affects women.
At the current rate, American women have a 1 in 8 risk of developing cancer by the age of 95 (American Cancer Society, Cancer Facts and 2s Figures, 1992, American Cancer Society, Atlanta, Georgia, USA).
Genetic factors contribute to an ill-defined proportion of breast cancer cases, estimated to be about 5 % of all cases but approximately 25 % of cases diagnosed before the age of 40 (Claus et al (1991) Ana J. Hung.
Gefaet. 48, 232-242). Breast cancer has been divided into two types, early-age onset and late stage onset, based on an inflection in the age specific incidence curve at around the age of 50. Mutation of one gene, BRCA 1, is thought to account for approximately 45 % of familial breast cancer, but at least 80 % of families with both breast and ovarian cancer s (Easton et al (1993) Am. J. Hum. Genet. 52, 678-701).
Breast carcinoma is potentially curable only when truly localised. The most common problem is either late presentation with overt metastases or, more frequently, the development of systemic metastases after apparent to local cure. Metastatic breast carcinoma is highly chemosensitive and effective chemotherapy routinely induces disease remission, allowing delay in the onset of secondary disease or amelioration of the symptoms of extensive disease.
Is Recently, the role of tumour-associated antigens in the biology of cancer has begun to be investigated. Probably the best studied example of tumour-associated antigens are the MAGE antigens which are involved in melanoma and certain other cancers, such as breast cancer. Therapeutic and diagnostic approaches making use of the MAGE antigens are 2o described in Gattoni-Celli & Cole (1996) Seminars in Oncology 23, 754-758, Itoh et al (1996) J. Biochena. 119, 385-390, WO 92/20356, WO
94/23031, WO 94/05304, WO 95/20974 and WO 95/23874. However, other tumour-associated antigens have also been implicated in breast cancer. For example, studies concerning the antigens expressed by breast 2s cancer cells, and in particular how these relate to the antigenic profile of the normal mammary epithelial cell, have been and continue to be a major activity in breast cancer research. The role of certain antigens in breast cancer, especially the role of polymorphic epithelial mucin (PEM; the product of the MUC 1 gene) and the .c-erbB2 protooncogene, are reviewed in Taylor-Papadimitriou et al (1993) Annals NY Acad. Sci. 698, 31-47.
Other breast cancer associated antigens include MAGE-1 and CEA.
Immunotherapeutic strategies and vaccines involving the MUC 1 gene or s PEM are described in Burchell et al (1996), pp 309-313, In Breast Cancer, Advances in Biology and Therapeutics, Calvo et al (eds), John Libbey Eurotext; Graham et al (1996) Int. J. Cancer 65, 664-670;
Graham et al (1995) Tunzor Targeting 1, 211-221; Finn et al (1995) Imrnunol. Rev. 145, 61-89; Burchell et al (1993) Cancer Surveys 18, 135-l0 148; Scholl & Pouillart (1997) Bull. Cancer 84, 61-64; and Zhang et al (1996) Cancej- Res. 56, 3315-3319.
Despite the recent interest in the breast cancer predisposing genes, BRCA1 and BRCA2, there remains the need for further information on is breast cancer, and the need for further diagnostic markers and targets for therapeutic intervention.
For cancers such as breast cancer, present screening methods are therefore unsatisfactory; there is no reliable method for diagnosing the cancer, or 2o predicting or preventing its possible metastatic spread, which is the main cause of death for most patients.
Grimes et al (1995) FEBS Lett. 369, 290-294 describes the differential expression of voltage-gated Na+ currents in two prostatic tumour cell lines 2s and discusses their contribution to invasiveness in vitro. The cell lines studied were rat cell lines and there is no indication of which particular voltage-gated Na+ channels may be involved.

Laniado et al (1997) Am J. Pathol. 150, 1213-1221 describes the expression and functional analysis of voltage-gated Na+ channels in human prostate cancer cell lines and discusses their contribution to invasion in vitf-o. There is no indication of which particular voltage-gated Na+
s channels may be involved.
Smith et al (1998) FEBS Lett. 423, 19-24 suggests that Na+ channel protein expression enhances the invasiveness of rat and human prostate cancer cell lines.
to Grimes & Djamgoz (1998) J. Cell. Physiol. 175, 50-58 describes the electrophysiological and pharmacological characterisation of voltage-gated Na+ current expressed in the highly metastatic Mat-LyLu cell line of rat prostate cancer. The underlying VGSC is identified as belonging to the is "tetrodotoxin-sensitive" class.
Dawes et al (1995) Visual Neuroscience 12, 1001-1005 describes the identification of voltage-gated Na+ channel subtypes induced in cultured retinal pigment epithelium cells.
Ul~ Patent application No 0021617.6 entitled "Diagnosis and treatment of cancer" filed on 2 September 2000 relates to methods of treatment and diagnosis of cancer, particularly prostate cancer concerning expression of VGSCs. VGSC expression correlates with pathological progression and a 2s VGSC which is associated with human cancer, particularly prostate cancer and its metastases, is hNe-Na (SCN9A). The amino acid sequence of the protein, and cDNA of the mRNA encoding it is known (I~lugbauer et al (1995) EMBO J. 14, 1084-1090).

Reviews of voltage-gated Na+ channels may be found in, for example, Black & Waxman (1996) Develop. Neurosci. 18, 139-152; Fozzard &
Hanck (1996) Physiol. Rev. 76, 887-926; Bullman (1997) Hurn. Mol.
Genet. 6, 1679-1685; Cannon (1999); Martian et al (1998) J. Physiol.
s 508, 647-657; Catterall (2000) Neuron 26, 13-25; Plummer & Meisler (1999) Geraornics 57, 323-331, and Goldin (2001) Ann Rev Physiol 63, 871-894. Some Na+ and other ion channels are well known to underly certain genetic defects as is described in Bullman (1997) Hum. Mol.
Genet. 6, 1679-1685; Burgess et al (1995) Nature Genet. 10, 461-465;
to and Cannon (1998) Mol Neurology (JB Martin, Ed) Scientific American Inc., NY.
The involvement of VGSCs in breast cancer has not been demonstrated, and the particular VGSC(s) involved in human breast cancer have not been 1s identified.
We have now found, surprisingly, that VGSC expression correlates with pathological progression and that VGSCs which are associated with human cancer, particularly breast cancer and its metastases, are SCNSA, SCNBA
2o and SCN9A, particularly SCNSA (also termed hl, SkM2 and Na,,l.5).
These are known VGSCs (although for SCNSA and SCNBA not previously known to be associated with human cancer, in particular human breast cancer) and amino acid sequences of the proteins, and cDNA of the mRNA encoding them have been reported (SCN9A: Klugbauer et al 2s (1995) EMB~ J. 14, 1084-1090, GenBank Accession No. X82835;
SCNSA: Gellens et al (1992) Proc. Natl. Acad. Sci. U.S.A. 89 (2), 554-558, GenBank Accession No. M77235; SCNBA: GenBank Accession No.
AB027567). Splice variants (for example neonatal splice variants;
discussed further below) and other variants of the reported SCNSA, SCNBA and SCN9A are included by the terms SCNSA, SCN8A and SCN9A. For example, sequences determined in the present work are included and particularly preferred, and are discussed in Example 1.
s The chromosomal location of SCN9A (also termed hNe-Na (human) and PNl (rat), and recently Na"1.7) has not yet been determined. However, the mouse equivalent has been located to the voltage-gated Na+ chalmel cluster on mouse chromosome 2 (Beckers et al (1997) Genornics 36, 202-205). This cluster is also present in human chromosome 2 where SCN9A
to may similarly be present (Malo et al (1994) Cytogeta. Cell. Genet. 67, 178-186; Malo et al (I994) Proc. Natl. Acad. Sci. USA 91, 2975-2979;
George et al (1994) Genomics 19, 395-397). The hNe-Na gene (human SCN9A) intron/exon organisation has not yet been determined but could be inferred from other known, conserved VGSC intron positions, as is reported in gene structure studies on SCN4A (George et al (1993) Gefionaics 15, 598-606), SCNSA (Wang et al (1996) Genomics 34, 9-16), SCNlOA (Sonslova et al (1997) Genomics 41, 201-209) and the Drosophila para VGSC gene (Loughey et al (I989) Cell 58, 1143-1154).
2o The brain-type voltage-gated Na+ channels (rat brain I-III (Noda et al (1986) Nature 322, 826-828; Kayano et al (2988) FEBS Lett. 228, 187-194) that are most similar to hNe-Na are 20 % different ~ over the whole sequence (human skeletal, 30 % ; heart 34 % different) . However, (i) if sequence comparison is made within specific structural/functional domains 2s this homology is much reduced (eg first one-third of DII-DIII cytoplasmic linker region is only 45 % homologous to the most similar channel (RBII/HBII); (ii) hNe-Na has sufficiently different regions (eg residues 446-460: EYTSIRRSRIMGLSE) to make specific antibodies (see, for example, Toledo-Aral et al (1997) Proc. Natl. Acad. Sci. USA 94, 1527-1532).
Subtype-specific antibodies for SCNSA are described in, for example, s Cohen & Levitt (1993) Circ Res 73, 735-742.
SCNgA is most similar to the brain-type VGSCs, sharing 70 % amino acid similarity (and approximately 60% similarity with other VGSCs). SCNSA
shares 60% similarity with most VGSCs, including the brain types.
It is an object of the invention to provide methods useful in providing diagnoses and prognoses of cancer, especially breast cancer, and for aiding the clinician in the management of cancer, particularly breast cancer. In particular, an object of the invention is to provide a method of is assessing the metastatic potential of cancer, in particular breast cancer.
Further objects of the invention include the provision of methods of treatment of cancer, in particular breast cancer, and methods of identifying compounds which selectively inhibit the VGSCs associated 2o with human cancer, particularly breast cancer, since these may be useful in treating cancer.
A first aspect of the invention provides a method of determining the susceptibility of a human patient to cancer comprising the steps of (i) 2s obtaining a sample containing nucleic acid and/or protein from the patient;
and (ii) determining whether the sample contains a level of SCNSA
voltage-gated Na+ channel nucleic acid or protein associated with cancer.

g A second aspect of the invention provides a method of diagnosing cancer in a human patient comprising the steps of (i) obtaining a sample containing nucleic acid and/or protein from the patient; and (ii) determining whether the sample contains a level of SCNSA voltage-gated s Na+ channel nucleic acid or protein associated with cancer.
It will be appreciated that determining whether the sample contains a level of SCNSA (or SCN9A in relation to the fourth and fifth aspects of the invention) VGSC nucleic acid or protein associated with cancer may in to itself be diagnostic of cancer or it may be used by the clinician as an aid in reaching a diagnosis.
For example, in relation to breast cancer, it is useful if the clinician undertakes a histopathological examination of biopsy tissue or carries out is external digital examination or carries out imaging. Clinical examination of breast cancer is done currently through morphological assessment of cells removed in a needle aspirate and also by mammography.
Mammography is also dependent on morphological changes on the mammogram. There is currently no biochemical assessment which is used 2o routinely to distinguish between cancer and non cancer in relation to breast cancer. Screening tests mentioned above relating to BRCA1 and BRCA2 may be used. It will be appreciated that the clinician will wish to take in to account these or other factors, as well as consider the level of a said VGSC, before making a diagnosis.
A third aspect of the invention provides a method of predicting the relative prospects of a particular outcome of a cancer in a human patient comprising the steps of (i) obtaining a sample containing nucleic acid and/or protein from the patient; and (ii) determining whether the sample contains a level of SCNSA voltage-gated Na+ channel nucleic acid or protein associated with cancer.
A fourth aspect of the invention provides a method of determining the s susceptibility of a human patient to breast cancer comprising the steps of (i) obtaining a sample containing nucleic acid and/or protein from the patient; and (ii) determining whether the sample contains a level of voltage-gated Na+ channel nucleic acid or protein associated with cancer.
Preferably the method comprises the step of determining whether the to sample contains a level of SCNSA and/or SCN9A voltage-gated Na+
channel nucleic acid or protein associated with cancer.
A fifth aspect of the invention provides a method of diagnosing breast cancer in a human patient comprising the steps of (i) obtaining a sample is containing nucleic acid and/or protein from the patient; and (ii) determining whether the sample contains a level of voltage-gated Na~*
channel nucleic acid or protein associated with cancer. Preferably the method comprises the step of determining whether the sample contains a Ievel of SCNSA and/or SCN9A voltage-gated Na+ channel nucleic acid or 2o protein associated with cancer.
A sixth aspect of the invention provides a method of predicting the relative prospects of a particular outcome of a breast cancer in a human patient comprising the steps of (i) obtaining a sample containing nucleic acid 2s and/or protein from the patient; and (ii) determining whether the sample contains a level of voltage-gated Na+ channel nucleic acid or protein associated with cancer. Preferably the method comprises the step of determining whether the sample contains a level of SCNSA and/or SCN9A
voltage-gated Nay channel nucleic acid or protein associated with cancer.

1~
Thus, the method of the third or sixth aspect of the invention may be useful in prognosis or aiding prognosis. The method may be used as an adjunct to known prognostic methods such as histopathological examination of biopsy tissue, external digital examination or imaging.
It is preferred for each of the preceding aspects of the invention, particularly the third and sixth aspects, that the method comprises the step of determining whether the sample contains a level of SCNSA voltage-zo gated Na+ channel nucleic acid or protein associated with cancer. The method may further comprise the step of determining whether the sample contains a level of SCN9A voltage-gated Na+ channel nucleic acid or protein associated with cancer.
is It will be appreciated that determination of the Ievel of a said VGSC
(including determination of the level of more than one, for example two said VGSCs) in the sample will be useful to the clinician in determining how to manage the cancer in the patient. For example, since elevated levels of a said VGSC, particularly SCNSA, are associated with metastatic 2o potential, particularly in a breast cancer, the clinician may use the information concerning the levels of the said VGSC(s) to facilitate decision making regarding treatment of the patient. Thus, if the level of said VGSC (preferably SCNSA) is indicative of a low metastatic potential of the cancer, preferably a breast cancer, unnecessary radical surgery may 2s be avoided. Similarly, if the level of said VGSC is indicative of a high metastatic potential of said cancer, preferably breast cancer, radical surgery (ie mastectomy) may be the preferred treatment. Even if it is not appropriate to alter the type of surgery carried out, determining whether the level of said VGSC is indicative of a high metastatic potential may help the clinician decide whether the patient needs adjuvant systemic treatment or not. At present, a major aim in oncology is to be able to distinguish those breast cancers with a high metastatic potential from those with a low metastatic potential, because those with a low metastatic s potential should not need to be put through six months of very toxic chemotherapy treatment.
It will be appreciated from the foregoing, and from the Examples below, that the determination of the levels of the said VGSC(s), preferably to SCNSA, may be exploited diagnostically to predict whether a given cancer, particularly breast cancer, would metastasise since expression of said VGSC, preferably SCNSA, is believed to correspond to possible, future spread of a tumour.
1 s It is particularly preferred if the cancer under consideration is breast cancer. Other appropriate cancers may include prostate cancer, small cell carcinoma of the lung and glioma (brain cancer).
It is also particularly preferred if the method of the invention is employed 20 to predict whether a given breast cancer would metastasise.
The Ievel of said VGSC which is indicative of cancer or metastatic potential may be defined as the increased level present in known cancerous or metastatic breast cells (preferably epithelial cells but possibly also or 2s alternatively other cell types such as neuroendocrine or myoepithelial cells) over known non-cancerous or non-metastatic breast cells. The level of said VGSC protein may be, for example, at least 11/a fold higher in cancerous cells or metastatic cells, or it may be at least 2-fold or 3-fold higher. Quantitative analysis by micro-densitometry of immunohistochemically processed tissue sections may be used. An antibody that is believed to react with all VGSCs may be used, possibly in combination with PCR analysis, which may be capable of distinguishing between VGSC types. The level of mRNA encoding said VGSC may be, s for example, at least 11/z fold higher in cancerous cells or metastatic cells, or it may be at least 2-fold or 3-fold higher, or at least 10, 50, 100, 500, 1000, 1200, 1500 or 1800-fold higher. Measurements by semi-quantitative PCR indicates that the level of SCNSA mRNA is about 1800-fold higher in the highly metastatic cell lines than in the lowly-metastatic cell lines, as zo described in the Examples.
In one preferred embodiment of the invention it is determined whether the level of said VGSC (preferably SCNSA) nucleic acid, in particular mRNA, is a level associated with cancer or metastatic potential.
zs Preferably, the sample contains nucleic acid, such as mRNA, and the level of said VGSC is measured by contacting said nucleic acid with a nucleic acid which hybridises selectively to said VGSC nucleic acid.
By "selectively hybridising" is meant that the nucleic acid has sufficient 2o nucleotide sequence similarity with the said human nucleic acid that it can hybridise under moderately or highly stringent conditions. As is well known in the art, the stringency of nucleic acid hybridization depends on factors such as length of nucleic acid over which hybridisation occurs, degree of identity of the hybridizing sequences and on factors such as 2s temperature, ionic strength and CG or AT content of the sequence. Thus, any nucleic acid which is capable of selectively hybridising as said is useful in the practice of the invention.

Nucleic acids which can selectively hybridise to the said human nucleic acid include nucleic acids which have > 95 % sequence identity, preferably those with > 9~ % , more preferably those with > 99 % sequence identity, over at least a portion of the nucleic acid with the said human nucleic acid.
s As is well known, human genes usually contain introns such that, for example, a mRNA or cDNA derived from a gene would not match perfectly along its entire length with the said human genomic DNA but would nevertheless be a nucleic acid capable of selectively hybridising to the said human DNA. Thus, the invention specifically includes nucleic 1o acids which selectively hybridise to said VGSC mRNA or cDNA but may not hybridise to a said VGSC gene. For example, nucleic acids which span the intron-axon boundaries of the said VGSC gene may not be able to selectively hybridise to the said VGSC mRNA or cDNA.
Is Typical moderately or highly stringent hybridisation conditions which lead to selective hybridisation are known in the art, for example those described in Molecular Cloning, a laboratory manual, 2nd edition, Sambrook et al (ads), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, incorporated herein by reference.
An example of a typical hybridisation solution when a nucleic acid is immobilised on a nylon membrane and the probe nucleic acid is >_ 500 bases or base pairs is:
2s 6 x SSC (saline Nay citrate) 0.5 % Na+ dodecyl sulphate (SDS) 100 ~.glml denatured, fragmented salmon sperm DNA

The hybridisation is performed at 68°C. The nylon membrane, with the nucleic acid immobilised, may be washed at 68°C in 1 x SSC or, for high stringency, 0.1 x SSC.
s 20 x SSC may be prepared in the following way. Dissolve 175.3 g of NaCI and 88.2 g of Na+ citrate in 800 ml of H20. Adjust the pH to 7.0 with a few drops of a 10 N solution of NaOH. Adjust the volume to 1 litre with HZO. Dispense into aliquots. Sterilize by autoclaving.
1o An example of a typical hybridisation solution when a nucleic acid is immobilised on a nylon membrane and the probe is an oligonucleotide of between 15 and 50 bases is:
3.0 M trimethylammonium chloride (TMACl) is 0.01 M Na+ phosphate (pH 6.8) 1 mm EDTA (pH 7.6) 0.5 % SDS
100 ~g/ml denatured, fragmented salmon sperm DNA
0.1 % nonfat dried milk The optimal temperature for hybridization is usually chosen to be 5°C
below the T; for the given chain length. T; is the irreversible melting temperature of the hybrid formed between the probe and its target sequence. Jacobs et al (1988) Nucl. Acids Res. 16, 4637 discusses the 2s determination of T;s. The recommended hybridization temperature for 17-mers in 3 M TMACI is 48-50 ° C; for 19-mers, it is 55-57 ° C;
and for 20-mers, it is 58-66°C.

By "nucleic acid which selectively hybridises" is also included nucleic acids which will amplify DNA from the said VGSC mRNA by any of the well known amplification systems such as those described in more detail below, in particular the polymerase chain reaction (PCR). Suitable s conditions for PCR amplification include amplification in a suitable 1 x amplification buffer:
x amplification buffer is 500 mM KCl; 100 mM Tris.Cl (pH ~.3 at room temperature); 15 mM MgCl2; 0.1 % gelatin.
to A suitable denaturing agent or procedure (such as heating to 95 ° C) is used in order to separate the strands of double-stranded DNA.
Suitably, the annealing part of the amplification is between 37 °
C and 1 s 60 ° C , preferably 50 ° C .
Although the nucleic acid which is useful in the methods of the invention may be RNA or DNA, DNA is preferred. Although the nucleic acid which is useful in the methods of the invention may be double-stranded or 2o single-stranded, single-stranded nucleic acid is preferred under some circumstances such as in nucleic acid amplification reactions.
The nucleic acid which is useful in the methods of the invention may be any suitable size. However, fox certain diagnostic, probing or amplifying 2s purposes, it is preferred if the nucleic acid has fewer than 10 000, more preferably fewer than 1000, more preferably still from 10 to 100, and in further preference from 15 to 30 base pairs (if the nucleic acid is double-stranded) or bases (if the nucleic acid is single stranded). As is described more fully below, single-stranded DNA primers, suitable for use in a polymerase chain reaction, are particularly preferred.
The nucleic acid for use in the methods of the invention is a nucleic acid s capable of hybridising to the said VGSC mRNA or mRNAs. Fragments of the said VGSC genes and cDNAs derivable from the mRNA encoded by the said VGSC genes are also preferred nucleic acids for use in the methods of the invention.
It is particularly preferred if the nucleic acid for use in the methods of the invention is an oligonucleotide primer which can be used to amplify a portion of the said VGSC nucleic acid, particularly VGSC mRNA.
Nucleic acids for use in the invention may hybridise to more than one, for is example all, substantially all or a particular subset of VGSC rnRNAs. The SCNSA, SCNBA and SCN9A mRNAs are similar to, but distinct from other VGSC mRNAs. This is discussed further in Examples 1 and 2.
Thus the nucleic acid for use in the invention may hybridise to a part of VGSC mRNAs that encodes a region of the VGSC polypeptide that is 2o conserved between VGSCs, for example has the same amino acid sequence in all, substantially all or a particular subset of VGSCs.
Preferred nucleic acids for use in the invention are those that selectively hybridise to the SCNSA, SCNBA or SCN9A mRNA and do not hybridise to other VGSC mRNAs. Such selectively hybridising nucleic acids can be 2s readily obtained, for example, by reference to whether or not they hybridise to the said VGSC mRNA and not to other VGSC mRNAs.
For example, SCNSA may be distinguished from other VGSCas by possession of C-terminal PDT domains, as discussed in Example 1; a nucleic acid hybridising to a nucleic acid encoding at least part of this C-terminal region in combination with a nucleic acid hybridising to a nucleic acid encoding another (non-PDZ domain) portion of SCNSA may be specific for SCNSA. The nucleic acids may be part of the same nucleic s acid molecule or may be separate nucleic acid molecules.
Methods and nucleic acids as described, for example, in Example 1, may be used. In particular, a semi-quantitative PCR technique, for example as described in Example 1, may be used. Examples of selectively 1o hybridising nucleic acids for SCNSA, SCN~A and SCN9A are shown in Table 1.
The methods are suitable in respect of any cancer but it is preferred if the cancer is breast cancer. The cancer may be small cell carcinoma of the is lung or a glioma or ovarian cancer or prostate cancer. It will be appreciated that the methods of the invention include methods of prognosis and methods which aid diagnosis. It will also be appreciated that the methods of the invention are useful to the physician or surgeon in determining a course of management or treatment of the patient.
The diagnostic and prognostic methods of the invention are particularly suited to female patients.
It is preferred if the nucleic acid is derived from a sample of the tissue in 2s which cancer is suspected or in which cancer may be or has been found.
For example, if the tissue in which cancer is suspected or in which cancer may be or has been found is breast, it is preferred if the sample containing nucleic acid is derived from the breast (including armpit tissue, for example lymph node tissue) of the patient. Samples of breast may be I$
obtained by surgical excision, "true cut" biopsies, needle biopsy, nipple aspirate, aspiration of a lump or image-guided biopsy. The image may be generated by X-ray, ultrasound or (less preferably) technetium-99-labelled antibodies or antibody fragments which bind or locate selectively at the s breast. Magnetic resonance imaging (MRI) may be used to distinguish fibrosis from breast cancer.
The sample may be directly derived from the patient, for example, by biopsy of the tissue, or it may be derived from the patient from a site to remote from the tissue, for example because cells from the tissue have migrated from the tissue to other parts of the body. Alternatively, the sample may be indirectly derived from the patient in the sense that, for example, the tissue or cells therefrom may be cultivated in vitro, or cultivated in a xenograft model; or the nucleic acid sample may be one is which has been replicated (whether in vitro or in vivo) from nucleic acid from the original source from the patient. Thus, although the nucleic acid derived from the patient may have been physically within the patient, it may alternatively have been copied from nucleic acid which was physically within the patient. The tumour tissue may be taken from the 2o primary tumour or from metastases. The sample may be lymph nodes, lymph or blood and the spread of disease detected.
Conveniently, the nucleic acid capable of hybridising to the said VGSC
mRNA and which is used in the methods of the invention further 2s comprises a detectable label.
By "detectable label" is included any convenient radioactive label such as 32p~ asp or ass which can readily be incorporated into a nucleic acid molecule using well known methods; any convenient fluorescent or chemiluminescent label which can readily be incorporated into a nucleic acid is also included. In addition the term "detectable label" also includes a moiety which can be detected by virtue of binding to another moiety (such as biotin which can be detected by binding to stareptavidin); and a s moiety, such as an enzyme, which can be detected by virtue of its ability to convert a colourless compound into a coloured compound, or vice versa (for example, alkaline phosphatase can convert colourless o-nitrophenylphosphate into coloured o-nitrophenol). Conveniently, the nucleic acid probe may occupy a certain position in a fixed assay and to whether the nucleic acid hybridises to the said VGSC nucleic acid can be determined by reference to the position of hybridisation in the fixed assay.
The detectable label may also be a fluorophore-quencher pair as described in Tyagi & Framer (1996) Nature Biotechnology 14, 303-308.
is Other types of labels and tags are disclosed above. The nucleic acid may be branched nucleic acid (see Urdea et al (1991) Nucl. Acids Symposium Series 24, 197-200).
It will be appreciated that the aforementioned methods may be used for 2o presymptomatic screening of a patient who is in a risk group for cancer.
High risk patients for screening include patients over SO years of age or patients who carry a gene resulting in increased susceptibility (eg predisposing versions of BRCAI, BRCA2 or p53); patients with a family history of breast/ovarian cancer; patients with affected siblings;
2s nulliparous women; and women who have a long interval between menarche and menopause. Similarly, the methods may be used for the pathological classification of tumours such as breast tumours.

Conveniently, in the methods of the invention the nucleic acid which is capable of the said selective hybridisation (whether labelled with a detectable label or not) is contacted with nucleic acid (eg mRNA) derived from the patient under hybridising conditions. Suitable hybridising s conditions include those described above.
The presence of a complex which is selectively formed by the nucleic acid hybridising to the VGSC mRNA may be detected, for example the complex may be a DNA:RNA hybrid which can be detected using 1o antibodies. Alternatively, the complex formed upon hybridisation may be a substrate for an enzymatic reaction the product of which may be detected (suitable enzymes include polymerases, ligases and endonucleases).
It is preferred that if the sample containing nucleic acid (eg mRNA) is derived from the patient is not a substantially pure sample of the tissue or cell type in question that the sample is enriched for the said tissue or cells.
For example, enrichment for breast cells in a sample such as a blood sample may be achieved using, for example, cell sorting methods such as fluorescent activated cell sorting (FACS) using a breast cell-selective 2o antibody, or at least an antibody which is selective for an epithelial cell.
For example, anti-MUCl antibodies such as HMFG-1 and HMFG-2 may be used (Taylor-Papadimitriou et al (1986) J. Exp. Pathol. 2, 247-260);
other anti-MUC 1 antibodies which may be useful are described in Cao et al (1998) Tumour Biol. 19, (Suppl 1), 88-99. The source of the said 2s sample also includes biopsy material as discussed above and tumour samples, also including fixed paraffin mounted specimens as well as fresh or frozen tissue. The nucleic acid sample from the patient may be processed prior to contact with the nucleic acid which selectively hybridises to the VGSC mRNA. For example, the nucleic acid sample from the patient may be treated by selective amplification, reverse transcription, immobilisation (such as sequence specific immobilisation), or incorporation of a detectable marker.
s Cells may be analysed individually, for example using single-cell immobilisation techniques. Methods by which single cells may be analysed include methods in which the technique of Laser Capture Microdissection (LCM) is used. This technique may be used to collect single cells or homogeneous cell populations for molecular analysis and is described in, to for example, Jin et al (1999) Lab Invest 79(4), 511-512; Simone et al (I99~) Trends Genet I4(7), 272-276; Luo et al (1999) Nature Med 5(I), 117-122; Arcuturs Updates, for example June 1999 and February 1999;
US 5,59,699 (all incorporated herein by reference). The cells of interest are visualised, for example by immunohistochemical techniques, and is transferred to a polymer film that is activated by laser pulses. The technique may also be used for isolation of cells which are negative for a particular component. Microscopes useful in performing LCM are manufactured by Arcturus Engineering, Inc., 1220 Terra Bella Avenue, Mountain View, CA 94042, USA.
LCM may be used with other isolation or enrichment methods. For example, LCM may be used following a method which enriches the sample for the target cell type.
2s It will be appreciated that the VGSC mRNA may be identified by reverse-transcriptase polymerase chain reaction (RT-PCR) using methods well known in the art.

Primers which are suitable for use in a polymerise chain reaction (PCR;
Saiki et al (1988) Science 239, 487-491) are preferred. Suitable PCR
primers may have the following properties:
s It is well known that the sequence at the 5' end of the oligonucleotide need not match the target sequence to be amplified.
It is usual that the PCR primers do not contain any complementary structures with each other longer than 2 bases, especially at their 3' ends, to as this feature may promote the formation of an artifactual product called "primer dimer" . When the 3' ends of the two primers hybridize, they form a "primed template" complex, and primer extension results in a short duplex product called "primer dimer".
is Internal secondary structure should be avoided in primers. For symmetric PCR, a 40-60% G+C content is often recommended for both primers, with no long stretches of any one base. The classical melting temperature calculations used in conjunction with DNA probe hybridization studies often predict that a given primer should anneal at a specific temperature or 2o that the 72 ° C extension temperature will dissociate the primer/template hybrid prematurely. In practice, the hybrids are more effective in the PCR process than generally predicted by simple Tm calculations.
Qptimum annealing temperatures may be determined empirically and may 2s be higher than predicted. Taq DNA polymerise does have activity in the 37-55 ° C region, so primer extension will occur during the annealing step and the hybrid will be stabilized. The concentrations of the primers are equal in conventional (symmetric) PCR and, typically, within 0.1- to 1-~M range.

Any of the nucleic acid amplification protocols can be used in the method of the invention including the polymerase chain reaction, QB replicase and ligase chain reaction. Also, NASBA (nucleic acid sequence based s amplification), also called 3SR, can be used as described in Compton (1991) Nature 350, 9I-92 and AIDS (1993), Vol 7 (Suppl 2), 5108 or SDA (strand displacement amplification) can be used as described in Walker et al (1992) Nucl. Acids Res. 20, 1691-1696. The polymerase chain reaction is particularly preferred because of its simplicity.
When a pair of suitable nucleic acids of the invention are used in a PCR it is convenient to detect the product by gel electrophoresis and ethidium bromide staining. As an alternative to detecting the product of DNA
amplification using agarose gel electrophoresis and ethidium bromide is staining of the DNA, it is convenient to use a labelled oligonucleotide capable of hybridising to the amplified DNA as a probe. When the amplification is by a PCR the oligonucleotide probe hybridises to the interprimer sequence as defined by the two primers. The oligonucleotide probe is preferably between 10 and 50 nucleotides long, more preferably 2o between 15 and 30 nucleotides long. ~ The probe may be labelled with a radionuclide such as 32P, asp and 35S using standard techniques, or may be labelled with a fluorescent dye. When the oligonucleotide probe is fluorescently labelled, the amplified DNA product may be detected in solution (see for example Balaguer et al (1991) "Quantification of DNA
2s sequences obtained by polymerase chain reaction using a bioluminescence adsorbent" Anal. Biocherra. 195, 105-110 and Dilesare et al (1993) "A
high-sensitivity electrochemiluminescence-based detection system for automated PCR product quantitation" BioTeclZfaiques 15, 152-157.

PCR products can also be detected using a probe which may have a fluorophore-quencher pair or may be attached to a solid support or may have a biotin tag or they may be detected using a combination of a capture probe and a detector probe.
s Fluorophore-quencher pairs are particularly suited to quantitative measurements of PCR reactions (eg RT-PCR). Fluorescence polarisation using a suitable probe may also be used to detect PCR products.
to Oligonucleotide primers can be synthesised using methods well known in the art, for example using solid-phase phosphoramidite chemistry.
The present invention provides the use of a nucleic acid which selectively hybridises to SCNSA nucleic acid (eg mRNA) in a method of diagnosing is cancer or prognosing cancer or determining susceptibility to cancer (preferably breast cancer); or in the manufacture of a reagent for carrying out these methods. The present invention further provides the use of a nucleic acid which selectively hybridises to VGCS nucleic acid (eg mRNA), preferably SCNSA andlor SCN9A nucleic acid, in a method of 2o diagnosing breast cancer or prognosing breast cancer or determining susceptibility to breast cancer; or in the manufacture of a reagent for carrying out these methods.
Other methods of detecting mRNA levels are included.
Methods for determining the relative amount of the said VGSC mRNA
include: in situ hybridisation (In Situ Hybridization Protocols. Methods in Molecular Biology Volume 33. Edited by K H A Choo. 1994, Humana Press Inc (Totowa, NJ, USA) pp 480p and In Situ Hybridization:

A Practical Approach. Edited by D G Wilkinson. 1992, Oxford University Press, Oxford, pp 163), in situ amplification, northerns, nuclease protection, probe arrays, and amplification based systems;
s The mRNA may be amplified prior to or during detection and quantitation. 'Real time' amplification methods wherein the product is measured for each amplification cycle may be particularly useful (eg Real time PCR Hid et al (1996) Genome Research 6, 956-994, Gibson et al (1996) Genome Research 6, 995-1001; Real time NASBA Oehlenschlager to et al (1996 Nov 12) PNAS (USA) 93(23), 12511-6. Primers should be designed to preferentially amplify from an mRNA template rather than from the DNA, or be designed to create a product where the mRNA or DNA template origin can be distinguished by size or by probing. NASBA
may be particularly useful as the process can be arranged such that only Is RNA is recognised as an °initial substrate.
Detecting mRNA includes detecting mRNA in any context, or detecting that there are cells present which contain mRNA (for example, by in situ hybridisation, or in samples obtained from lysed cells). It is useful to 2o detect the presence of mRNA or that certain cells are present (either generally or in a specific location) which can be detected by virtue of their expression of the said VGSC mRNA. As noted, the presence versus absence of the said VGSC mRNA may be a useful marker, or low levels versus high levels of the said VGSC mRNA may be a useful marker, or 2s specific quantified levels may be associated with a specific disease state.
It will be appreciated that similar possibilities exist in relation to using the said VGSC polypeptide as a marker.

In a further preferred embodiment, the level of said VGSC protein is measured. Preferably, the level of said protein is measured by contacting the protein with . a molecule which selectively binds to said VGSC
polypeptide.
The sample containing protein derived from the patient is conveniently a sample tissue. It may be useful to measure the presence (tumour) versus absence (normal) of the said VGSC polypeptide in some circumstances, such as when assessing breast tissue. The methods of the invention also 1o include the measurement and detection of the said VGSC polypeptide in test samples and their comparison in a control sample.
The sample containing protein derived from the patient is conveniently a sample of the tissue in which cancer is suspected or in which cancer may Is be or has been found. These methods may be used for any cancer, but they are particularly suitable in respect of breast cancer. Methods of obtaining suitable samples are described in relation to earlier methods.
The sample may also be blood, serum or lymph node-derived material which may be particularly useful in determining whether a cancer has 2o spread. Single cells may be analysed, as noted above.
The methods of the invention involving detection of the said VGSC
proteins are particularly useful in relation to historical samples such as those containing paraffin-embedded sections of tumour samples.
The level of said VGSC protein may be determined in a sample in any suitable way.

It is particularly preferred if the molecule which selectively binds to the said VGSC (for example all VGSCs or selected VGSC(s), for example SCNSA) is an antibody.
s Antibodies which can selectively bind to VGSCs or a particular form or forms of VGSC are described above and can be,made, for example, by using peptides which are respectively conserved in all or in particular VGSCs, or which encompass the differences between one form of VGSC
and the other forms. For example, SCNSA may be distinguished from to other VGSCas by possession of C-terminal PDZ domains, as discussed in Example 1; an antibody binding to part of this C-terminal region may be useful in distinguishing SCNSA from other VGSCs (for example in conjunction with an antibody binding to another portion of SCNSA (and other VGSCs)).
Is The antibodies may be monoclonal or polyclonal. Suitable monoclonal antibodies may be prepared by known techniques, for example those disclosed in "Monoclonal Antibodies: A manual of techniques", H Zola (CRC Press, 1988) and in "Monoclonal Hybridoma Antibodies:
2o Techniques and applications", J G R Hurrell (CRC Press, 1982), both of which are incorporated herein by reference.
The level of the said VGSC which is indicative of cancer or metastatic potential may be defined as the increased level present in known cancerous 2s or metastatic cells, preferably cancerous or metastatic breast cells over known non-cancerous or non-metastatic breast cells. The level may be, for example, at least 11/a fold higher in cancerous or metastatic cells, or it may be at least 2-fold or 3-fold higher.

By "the relative amount of said VGSC protein" is meant the amount of said VGSC protein per unit mass of sample tissue or per unit number of sample cells compared to the amount of said VGSC protein per unit mass of known normal tissue or per unit number of normal cells. The relative s amount may be determined using any suitable protein quantitation method.
In particular, it is preferred if antibodies are used and that the amount of said VGSC protein is determined using methods which include quantitative western blotting, enzyme-linked immunosorbent assays (ELISA) or quantitative immunohistochemistry.
to As noted above, an increased level of the said VGSC, for example SCNSA in a sample compared with a known normal tissue sample is suggestive of a turriorigenic sample, with high metastatic potential. In relation to breast tissue, the presence of the said VGSC (SCNSA andlor is SCN9A), compared to its absence, is suggestive of carcinogenesis.
Other techniques for raising and purifying antibodies are well known in the art and any such techniques may be chosen to achieve the preparations useful in the methods claimed in this invention. In a preferred embodiment 20 of the invention, antibodies will immunoprecipitate said VGSC proteins from solution as well as react with said VGSC protein on western or immunoblots of polyacrylamide gels. In another preferred embodiment, antibodies will detect said VGSC proteins in paraffin or frozen tissue sections, using immunocytochemical techniques.
Preferred embodiments relating to methods for detecting said VGSC
protein include enzyme linked immunosorbent assays (ELISA), radioimmunoassay (RIA), immunoradiometric assays (IRMA) and immunoenzymatic assays (IEMA), including sandwich assays using monoclonal and/or polyclonal antibodies. Exemplary sandwich assays are described by David et al in US Patent Nos. 4,376,110 and 4,486,530, hereby incorporated by reference.
s It will be appreciated that other antibody-like molecules may be used in the method of the inventions including, for example, antibody fragments or derivatives which retain their antigen-binding sites, synthetic antibody-like molecules such as single-chain Fv fragments (ScFv) and domain antibodies (dAbs), and other molecules with antibody-like antigen binding to motifs.
A further aspect of the invention provides the use of a molecule which selectively binds to SCNSA VGSC polypeptide (including a natural fragment or variant thereof) in a method of diagnosing cancer or ~s prognosing cancer or determining susceptibility to cancer (preferably breast cancer); or in the manufacture of a reagent for diagnosing cancer or prognosing cancer or determining susceptibility to cancer. The present invention further provides the use of a molecule which selectively binds to a VGSC polypeptide (including a natural fragment or variant thereof), 2o preferably SCNSA or SCN9A, in a method of diagnosing breast cancer or prognosing breast cancer or determining susceptibility to breast cancer; or in the manufacture of a reagent for carrying out these methods.
In a further embodiment the level of the said VGSC is measured by 2s selectively assaying its activity in the sample. The activity of VGSC, for example SCNSA VGSC, in a sample may be assayed by dissociating a biopsy into single cells and in culture assaying (i) the effect of voltage-gated Na+ channel blockers on their motility and (ii) detecting goltage-gated Na+ channel activity by electrophysiological recording. Suitable methods and voltage-gated Na+ channel blockers are described in Example 1.
Preferred diagnostic methods of the invention include what can broadly be s described as "invasive" methods and "non-invasive" methods. Invasive methods include, for example, the taking of a biopsy for detection of voltage-gated Na+ channel expression by, for example, (a) immunohistochemical application of a sequence-specific antibody, {b) in situ PCR on tissue sections, or (c) reverse transcription (RT)-PCR of To cells, for example epithelial cells (and/or other cell types, for example neuroendocrine or myoepithelial cells) after separating them from the biopsy. Non-invasive methods include obtaining breast-derived cells from blood, which may be separated by affinity and assayed for voltage-gated Na+ channel expression by PCR.
~s A further aspect of the invention provides the use of an agent which is an agent useful in selectively assaying the activity of SCNSA voltage-gated Na+ channel protein in a sample in a method of diagnosing cancer or prognosing cancer or determining susceptibility to cancer (preferably 2o breast cancer); or in the manufacture of a reagent for diagnosing cancer or prognosing cancer or determining susceptibility to cancer. The present invention further provides the use of an agent which is an agent useful in selectively assaying the activity of a voltage-gated Na+ channel protein, preferably SCNSA or SCN9A, in a sample in a method of diagnosing 2s breast cancer or prognosing breast cancer or determining susceptibility to breast cancer; or in the manufacture of a reagent for carrying out these methods .

The agents as defined are therefore useful in a method of diagnosing cancer.
A further aspect of the invention provides a kit of parts useful for s diagnosing cancer, especially breast cancer, comprising an agent which is capable of use in determining the level of SCNSA (and optionally SCN9A) VGSC protein or nucleic acid in a sample. The agent may be a nucleic acid which selectively hybridises to the said VGSC nucleic acid or the agent may be a molecule which selectively binds to the said VGSC protein or the agent may be an agent useful in selectively assaying the activity of the said VGSC.
Preferably, the kit further comprises a control sample containing the said VGSC nucleic acid or protein wherein the control sample may be a is negative control (which contains a level of the said VGSC protein or nucleic acid which is not associated with cancer or a high metastatic potential for cancer) or it may be a positive control (which contains a level of the said VGSC protein or nucleic acid which is associated with cancer or a high metastatic potential for cancer). The kit may contain both 2o negative and positive controls. The kit may usefully contain controls of the said VGSC protein or nucleic acid which correspond to different amounts such that a calibration curve may be made.
Suitably, the kit further comprises means for separating breast cells (for 2s example epithelial cells, neuroendocrine or myoepithelial cells) from a sample in order to carry out said VGSC assay. Preferably, the means for separating breast cells includes antibody-coated micro-beads or columns.
These are coated with antibodies to cell membrane proteins. For example, as noted above, anti-MUC1 antibodies such as HMFG-1 and HMFG-2 may be used (Taylor-Papadimitriou et al (1986) J. Exp. Pathol. 2, 247-260); other anti-MUC 1 antibodies which may be useful are described in Cao et al (1998) Tumour Biol. 19, (Suppl 1), 88-99. However, anti-MUC1 antibodies may bind to normal bone marrow cells. It is preferred s to use an anti epithelial cell adhesion molecule antibody, preferably coated on magnetic beads. A preferred antibody is termed BER-EP-4.
A further aspect of the invention provides a kit of parts useful for diagnosing breast cancer, comprising (1) an agent which is capable of use to in determining the level of VGSC, preferably SCNSA or SCN9A, protein or nucleic acid in a sample, and (2) means for separating breast cells (for example epithelial cells, neuroendocrine or myoepithelial cells) from a sample in order to carry out said VGSC assay.
15 The kits may usefully further comprise a component for testing for a further cancer-related polypeptide such as antibodies which are reactive with one or more of the following cancer-related polypeptides, aII of which are well known in the art: MAGE-1, MAGE-3, BAGE, GAGE-l, CAG-3, CEA, p53, oestrogen receptor (ER), progesterone receptor (PR), 2o MUC1, p52 trefoil peptide, Her2, PCNA, I~i67, cyclin D, p90r~'3, p170 glycoprotein (mdr-1) CA-15-3, c-erbBl, cathepsin D, PSA, CA125, CA19-9, PAP, myc, cytokeratins, bcl-2, telomerase, glutathione S
transferases, radSl, VEGF, thymidine phosphorylase, FIkI. or Flk2.
2s The kit may usefully still further or alternatively comprise a nucleic acid which selectively hybridises to a further cancer-related nucleic acid such as a gene or mRNA which encodes any of the cancer-related polypeptides as described above. In addition, useful nucleic acids which may be included in the kit are those which selectively hybridise with the genes or mRNAs: ras, APC, BRCAl, BRCA2, ataxia telangiectasia (ATM), hMSH2, hMCHl , hPMS2 or hPMS 1. It is preferred if the further nucleic acid is one which selectively hybridises to the gene or mRNA of any of erbB2, p53, BRCA1, BRCA2 or ATM.
The kits usefully may contain controls and detection material, (for example, for immunohistochemistry, secondary antibodies labelled fluorophores, or enzymes, or biotin, or digoxygenin or the like). For immunoassays, additional components to the kit may include a second to antibody to a different epitope on the VGSC (optionally labelled or attached to a support), secondary antibodies (optionally labelled or attached to a support), and dilution and reaction buffers. Similar additional components may usefully be included in all of the kits of the invention.
is A further aspect of the invention provides a method of treating cancer comprising the step of administering to the patient an agent which selectively prevents the function of SCNSA (and optionally also SCN9A) voltage-gated Nay channel.
A further aspect of the invention provides a method of treating breast cancer comprising the step of administering to the patient an agent which selectively prevents the function of a voltage-gated Na+ channel, preferably SCNSA or SCN9A, still more preferably selectively SCNSA or Zs SCN9A.
By "an agent which selectively prevents the function of a voltage-gated Nay channel" we include agents that (a) inhibit the expression of a said VGSC or (b) inhibit the activity of a said VGSC.

Agents that prevent the expression of said VGSC include but are not limited to antisense agents and ribozymes.
s Antisense oligonucleotides are single-stranded nucleic acid, which can specifically bind to a complementary nucleic acid sequence. By binding to the appropriate target sequence, an RNA-RNA, a DNA-DNA, or RNA-DNA duplex is formed. These nucleic acids are often termed "antisense"
because they are complementary to the sense or coding strand of the gene.
to Recently, formation of a triple helix has proven possible where the oligonucleotide is bound to a DNA duplex. It was found that oligonucleotides could recognise sequences in the major groove of the DNA double helix. A triple helix was formed thereby. This suggests that it is possible to synthesise sequence-specific molecules which specifically Is bind double-stranded DNA via recognition of major groove hydrogen binding sites.
By binding to the target nucleic acid, the above oligonucleotides can inhibit the function of the target nucleic acid. This could, for example, be 2o a result of blocking the transcription, processing, poly(A)addition, replication, translation, or promoting inhibitory mechanisms of the cells, such as promoting RNA degradations.
Antisense oligonucleotides are prepared in the laboratory and then 2s introduced into cells, for example by microinjection or uptake from the cell culture medium into the cells, or they are expressed in cells after transfection with plasmids or retroviruses or other vectors carrying an antisense gene. Antisense oligonucleotides were first discovered to inhibit viral replication or expression in cell culture for Rous sarcoma virus, vesicular stomatitis virus, herpes simplex virus type 1, simian virus and influenza virus. Since then, inhibition of mRNA translation by antisense oligonucleotides has been studied extensively in cell-free systems including rabbit reticulocyte lysates and wheat germ extracts. Inhibition s of viral function by antisense oligonucleotides has been demonstrated in vitro using oligonucleotides which were complementary to the AIDS HIV
retrovirus RNA (Goodchild, J. 1988 "Inhibition of Human Immunodeficiency Virus Replication by Antisense Oligodeoxynucleotides", Proc. Natl. Acad. Sci. (USA) 85(15), 5507-11).
to The Goodchild study showed that oligonucleotides that were most effective were complementary to the poly(A) signal; also effective were those targeted at the 5' end of the RNA, particularly the cap and 5' untranslated region, next to the primer binding site and at the primer binding site. The cap, 5' untranslated region, and poly(A) signal lie within the sequence is repeated at the ends of retrovirus RNA (R region) and the oligonucleotides complementary to these may bind twice to the RNA.
Oligonucleotides are subject to being degraded or inactivated by cellular endogenous nucleases. To counter this problem, it is possible to use 2o modified oligonucleotides, eg having altered internucleotide linkages, in which the naturally occurring phosphodiester linkages have been replaced with another linkage. For example, Agrawal et al (1988) Proc. Natl. Acad.
Sci. USA 85, 7079-7083 showed increased inhibition in tissue culture of HIV-1 using oligonucleotide phosphoramidates and phosphorothioates.
2s Sarin et al (1988) Proc. Natl. Acad. Sci. USA 85, 7448-7451 demonstrated increased inhibition of HIV-1 using oligonucleotide methylphosphonates.
Agrawal et al (1989) Proc. Natl. Acad. Sci. USA 86, 7790-7794 showed inhibition of HIV-1 replication in both early-infected and chronically infected cell cultures, using nucleotide sequence-specific oligonucleotide phosphorothioates. Leither et al (1990) Proc. Natl. Acad. Sci. USA 87, 3430-3434 report inhibition in tissue culture of influenza virus replication by oligonucleotide phosphorothioates.
s Oligonucleotides having artificial linkages have been shown to be resistant to degradation in vivo. For example, Shaw et al (1991) in Nucleic Acids Res. 19, 747-750, report that otherwise unmodified oligonucleotides become more resistant to nucleases in vivo when they are blocked at the 3' end by certain capping structures and that uncapped oligonucleotide to phosphorothioates are not degraded in vivo.
A detailed description of the H-phosphonate approach to synthesizing oligonucleoside phosphorothioates is provided in Agrawal and Tang (1990) Tetrahedron Letters 31, 7541-7544, the teachings of which are hereby is incorporated herein by reference. Syntheses of oligonucleoside methylphosphonates, phosphorodithioates, phosphoramidates, phosphate esters, bridged phosphoramidates and bridge phosphorothioates are known in the art. See, for example, Agrawal and Goodchild (1987) Tetrahedron Letters 28, 3539; Nielsen et al (1988) Tetralaedrora Letters 29, 2911; Jager 2o et al (1988) Biochemistry 27, 7237; Uznanski et al (1987) Tetrahedron Letters 28, 3401; Bannwarth (1988) Helv. C'hirra. Acta. 71, 1517; Crosstick and Vyle (1989) Tetrahedron Letters 30, 4693; Agrawal et al (1990) Proc.
Natl. Acad. Sci. USA 87, 1401-1405, the teachings of which are incorporated herein by reference. Other methods for synthesis or 2s production also are possible. In a preferred embodiment the oligonucleotide is a deoxyribonucleic acid (DNA), although ribonucleic acid (RNA) sequences may also be synthesized and applied.

The oligonucleotides useful in the invention preferably axe designed to resist degradation by endogenous nucleolytic enzymes. In vivo degradation of oligonucleotides produces oligonucleotide breakdown products of reduced length. Such breakdown products axe more likely to engage in non-specific s hybridization and are less likely to be effective, relative to their full-length counterparts. Thus, it is desirable to use oligonucleotides that are resistant to degradation in the body and which are able to reach the targeted cells.
The present oligonucleotides can be rendered more resistant to degradation in vivo by substituting one or more internal artificial internucleotide linkages to for the native phosphodiester linkages, for example, by replacing phosphate with sulphur in the linkage. Examples of linkages that may be used include phosphorothioates, methylphosphonates, sulphone, sulphate, ketyl, phosphorodithioates, various phosphoramidates, phosphate esters, bridged phosphorothioates and bridged phosphoramidates. Such examples are is illustrative, rather than limiting, since other internucleotide linkages are known in the art. See, for example, Cohere, (1990) Trends in Biotechnology. The synthesis of oligonucleotides having one or more of these linkages substituted for the phosphodiester internucleotide linkages is well known in the art, including synthetic pathways for producing 20 oligonucleotides having mixed internucleotide linkages.
Oligonucleotides can be made resistant to extension by endogenous enzymes by "capping" or incorporating similar groups on the 5' or 3' terminal nucleotides. A reagent for capping is commercially available as Amino-2s Link IITM from Applied BioSystems Inc, Foster City, CA. Methods for capping are described, for example, by Shaw et al (1991) Nucleic Acids Res. 19, 747-750 and Agrawal et al (1991) Proc. Natl. Acad. Sci. ZJSA
88(17), 7595-7599, the teachings of which are hereby incorporated herein by reference.

A further method of making oligonucleotides resistant to nuclease attack is for them to be "self stabilized" as described by Tang et al (1993) Nucl.
Acids Res. 21, 2729-2735 incorporated herein by reference. Self stabilized s oligonucleotides have hairpin loop structures at their 3' ends, and show increased resistance to degradation by snake venom phosphodiesterase, DNA polymerase I and fetal bovine serum. The self stabilized region of the oligonucleotide does not interfere in hybridization with complementary nucleic acids, and pharmacokinetic and stability studies in mice have shown increased in vivo persistence of self stabilized oligonucleotides with respect to their linear counterparts.
In accordance with the invention, the inherent binding specificity of antisense oligonucleotides characteristic of base pairing is enhanced by is limiting the availability of the antisense compound to its intend locus in vivo, permitting lower dosages to be used and minimizing systemic effects.
Thus, oligonucleotides axe applied locally to achieve the desired effect. The concentration of the oligonucleotides at the desired locus is much higher than if the oligonucleotides were administered systemically, and the 2o therapeutic effect can be achieved using a significantly lower total amount.
The local high concentration of oligonucleotides enhances penetration of the targeted cells and effectively blocks translation of the target nucleic acid sequences.
2s The oligonucleotides can be delivered to the locus by any means appropriate for localized administration of a drug. For example, a solution of the oligonucleotides can be injected directly to the site or can be delivered by infusion using an infusion pump. The oligonucleotides also can be incorporated into an implantable device which when placed at the desired site, permits the oligonucleotides to be released into the surrounding locus.
The oligonucleotides may be administered via a hydrogel material. The s hydrogel is noninflammatory and biodegradable. Many such materials now are known, including those made from natural and synthetic polymers. In a preferred embodiment, the method exploits a hydrogel which is liquid below body temperature but gels to form a shape-retaining semisolid hydrogel at or near body temperature, Preferred hydrogel are polymers of ethylene oxide-to propylene oxide repeating units. The properties of the polymer are dependent on the molecular weight of the polymer and the relative percentage of polyethylene oxide and polypropylene oxide in the polymer.
Preferred hydrogels contain from about 10 to about ~0 % by weight ethylene oxide and from about 20 to about 90 % by weight propylene oxide. A
1 s particularly preferred hydrogel contains about 70 % polyethylene oxide and 30 % polypropylene oxide. Hydrogels which can be used are available, for example, from BASF Corp., Parsippany, NJ, under the tradename PluronicR.
2o In this embodiment, the hydrogel is cooled to a liquid state and the oligonucleotides are admixed into the liquid to a concentration of about 1 mg oligonucleotide per gram of hydrogel. The resulting mixture then is applied onto the surface to be treated, for example by spraying or painting during surgery or using a catheter or endoscopic procedures. As the 2s polymer warms, it solidifies to form a gel, and the oligonucleotides diffuse out of the gel into the surrounding cells over a period of time defined by the exact composition of the gel.

The oligonucleotides can be administered by means of other implants that are commercially available or described in the scienti~lc literature, including liposomes, microcapsules and implantable devices. For example, implants made of biodegradable materials such as polyanhydrides, polyorthoesters, s polylactic acid and polyglycolic acid and copolymers thereof, collagen, and protein polymers, or non-biodegradable materials such as ethylenevinyl acetate (E~Ac), polyvinyl acetate, ethylene vinyl alcohol, and derivatives thereof can be used to locally deliver the oligonucleotides. The oligonucleotides can be incorporated into the material as it is polymerized or to solidified, using melt or solvent evaporation techniques, or mechanically mixed with the material. In one embodiment, the oligonucleotides are mixed into or applied onto coatings for implantable devices such as dexixan coated silica beads, stents, or catheters. Polymeric nanoparticles/biodegradable drug carriers may also be used (Mader (1998) is Raeliol. Oncol. 32, 89-94).
The dose of oligonucleotides is dependent on the size of the oligonucleotides and the purpose for which it is administered. In general, the range is calculated based on the surface area of tissue to be treated. The effective 2o dose of oligonucleotide is somewhat dependent on the length and chemical composition of the oligonucleotide but is generally in the range of about 30 to 3000 ~,g per square centimetre of tissue surface area.
The oligonucleotides may be administered to the patient systemically for 2s both therapeutic and prophylactic puzposes. The oligonucleotides may be administered by any effective method, for example, parenterally (eg intravenously, subcutaneously, intramuscularly) or by oral, nasal or other means which permit the oligonucleotides to access and circulate in the patient's bloodstream. Oligonucleotides administered systemically preferably are given in addition to locally administered oligonucleotides, but also have utility in the absence of local administration. A dosage in the range of from about 0.1 to about 10 grams per administration to an adult human generally will be effective for this purpose.
s It will be appreciated that it may be desirable to target the antisense oligonucleotides to the cancerous tissue, for example to the breast. This may be achieved by administering the antisense oligonucleotides to the cancer location (for example the breast), or it may be achieved by using to antisense oligonucleotides which are in association with a molecule which selectively directs the antisense oligonucleotide to the cancer location. For example, the antisense oligonucleotide may be associated with an antibody or antibody like molecule which selectively binds a breast-related antigen such as MUC-1. By "associated with" we mean that the antisense ~s oligonucleotide and the cancer-directing entity are so associated that the cancer-directing entity is able to direct the antisense oligonucleotide to the location of the cancer cells, for example breast cells.
It will be appreciated that antisense agents also include larger molecules, for 2o example of around one hundred to several hundred bases which bind to said VGSC mRNA or genes and substantially prevent expression of said VGSC
mRNA or genes and substantially prevent expression of said VGSC protein.
Thus, expression of an antisense molecule which is substantially complementary to said VGSC mRNA is envisaged as part of the invention.
Thus, in this approach, synthetic oligonucleotides with antisense sequence to specific regions of (i) SCNSA (and optionally also SCN9A) channels or (ii) (for patients with or at risk of breast cancer) SCNSA or SCN9A channels or VGSCs generally, are administered (preferably to patients with or at risk of breast cancer) to block channel activity. Details of particular synthetic oligonucleotides are given in Example 2. It is noteworthy that antisense oligonucleotide technology has already been used to manipulate potassium channels in vitro (Roy et al (1996) Glia 18, 174-188) and VGSCs in vitro s (Biochem Biophys Res Comm (1997) 234, 235-241) and in blocking neuropathic pain (Lai et al (1999) "Blockade of neuropathic pain by antisense targeting of tetrodotoxin-resistant sodium channels in sensory neurons" Methods in Enzymol 314, 201-213).
1o A further method for blocking said VGSC activity includes dominant negative suppression. In this technique, a mutated VGSC gene product suppresses or eliminates the activity of the corresponding normal gene product when the two are co-expressed. In the case of voltage-gated potassium channels (VGPCs) which comprise 4 alpha subunits, such an Is approach making use of a highly truncated gene product, has been used successfully to suppress functional expression of VGPCs in vitro (Tu et al (1995) Biophys. J. 68, 147-156) and in vivo (London et al (1998) Proc.
Natl. Acad. Sci. USA 95, 2926-2931). The truncated subunit is capable of binding to other VGPC subunits but does not contain the residues required 2o for channel functioning. Thus, the activity of the "combined" VGPC is blocked. A number of naturally occurring alternatively spliced channel subunits have been detected which may function to suppress VGPC activity by a similar mechanism in vivo (Baumann et al (1987) EMBO J. 6, 3419-3429; Kamb et al (1988) Neuron I, 42I-430; and Pongs et al (I988) EMBO
2s J. 7, 1087-1096). We believe that VGSC may similarly be suppressed by interfering with functional channel formation. Although VGSCs are formed from a single alpha subunit (comprising four functional domains), recent studies have demonstrated the specific expression (during development in human, mouse and fish) of truncated VGSC proteins possessing only two domains which might function in a dominant negative manner to control VGSC activity (Plummer et al (1997) J. Biol. Claern. 272, 24008-24015;
and Oh & Waxman (1998) NeuroReport 9, 1267-1271). The neonatal VGSCs may act as inhibitors of VGSC activity, but this inhibition is most s probably specific to the related adult VGSC. It is much less likely that, for example, neonatal SCNBA could inhibit the activity of VGSC proteins derived from VGSC genes other than SCNBA.
The larger molecules may be expressed from any suitable genetic. construct to as is described below and delivered to the patient. Typically, the genetic construct which expresses the antisense molecule comprises at least a portion of the said VGSC cDNA or gene operatively linked to a promoter which can express the antisense molecule in the cell, preferably breast cell, which is or may become cancerous.
is Although the genetic construct can be DNA or RNA it is preferred if it is DNA.
Preferably, the genetic construct is adapted for delivery to a human cell.
Means and methods of introducing a genetic construct into a cell in an animal body are known in the art. For example, the constructs of the invention may be introduced into the tumour cells by any convenient method, for example methods involving retroviruses, so that the construct 2s is inserted into the genome of the, tumour cell. For example, in Kuriyama et al (1991) Cell Struc. and Func. 16, 503-510 purified retroviruses are administered. Retroviruses provide a potential means of selectively infecting cancer cells because they can only integrate into the genome of dividing cells; most normal cells surrounding cancers are in a quiescent, non-receptive stage of cell growth or, at least, are dividing much less rapidly than the tumour cells. Retroviral DNA constructs which encode said antisense agents may be made using methods well known in the art.
To produce active retrovirus from such a construct it is usual to use an s ecotropic psi2 packaging cell line grown in Dulbecco's modified Eagle's medium (DMEM) containing 10% foetal calf serum (FCS). Transfection of the cell line is conveniently by calcium phosphate co-precipitation, and stable transformants are selected by addition of 6418 to a final concentration of 1 mg/ml (assuming the retroviral construct contains a to tzeoR gene). Independent colonies are isolated and expanded and the culture supernatant removed, filtered through a 0.45 ~.m pore-size filter and stored at -70 ° . For the introduction of the retrovirus into the tumour cells, it is convenient to inject directly retroviral supernatant to which 10 ~.g/ml Polybrene has been added. For tumours exceeding 10 mm in is diameter it is appropriate to inject between 0.1 ml and Z ml of retroviral supernatant; preferably 0.5 ml.
Alternatively, as described in Culver et al (I992) Science 256, 1550-1552, cells which produce retroviruses are injected into the tumour. The 2o retrovirus-producing cells so introduced are engineered to actively produce retroviral vector particles so that continuous productions of the vector occurred within the tumour mass in ,ritu. Thus, proliferating tumour. cells can be successfully transduced in vivo if mixed with retroviral vector-producing cells.
as Targeted retroviruses are also available for use in the invention; for example, sequences conferring specific binding affinities may be engineered into preexisting viral env genes (see Miller & Vile (1995) Faseb J. 9, 190-199 for a review of this and other targeted vectors for gene therapy).
Other methods involve simple delivery of the construct into the cell for s expression therein either for a limited time or, following integration into the genome, for a longer time. An example of the latter approach includes (preferably tumour-cell-targeted) liposomes (Nassander et al (1992) Cancer ReS. 52, 646-653).
1o Immunoliposomes (antibody-directed liposomes) are especially useful in targeting to cancer cell types which over-express a cell surface protein for which antibodies are available. For example, the immunoliposomes may be targeted by means of antibodies binding to a breast cancer cell antigen such as MLTC-1, or the said VGSC (preferably in combination with other is targeting means or methods), as discussed further below. For the preparation of immuno-liposomes MPB-PE (N-[4-(p-maleimidophenyl)butyryl]-phosphatidylethanolamine) is synthesised according to the method of Martin 8z Papahadjopoulos (1982) J. Biol.
Clzem. 257, 286-288. MPB-PE is incorporated into the liposomal bilayers 2o to allow a covalent coupling of the antibody, or fragment thereof, to the liposomal surface. The liposome is conveniently loaded with the DNA or other genetic construct of the invention for delivery to the target cells, for example, by forming the said liposomes in a solution of the DNA or other genetic construct, followed by sequential extrusion through polycarbonate 2s membrane filters with 0.6 ~,m and 0.2 ~,m pore size under nitrogen pressures up to 0.8 MPa. After extrusion, entrapped DNA construct is separated from free DNA construct by ultracentrifugation at 80 000 x g for 45 min. Freshly prepared MPB-PE-liposomes in deoxygenated buffer are mixed with freshly prepared antibody (or fragment thereof] and the coupling reactions axe carried out in a nitrogen atmosphere at 4 ° C
under constant end over end rotation overnight. The immunoliposomes are separated from unconjugated antibodies by ultracentrifugation at 80 000 x g, for 45 min. Immunoliposomes may be injected intraperitoneally or s directly into the tumour.
Other methods of delivery include adenoviruses carrying external DNA
via an antibody-polylysine bridge (see Curiel Prog. Meel. hirol. 40, 1-18) and transferrin-polycation conjugates as carriers (Wagner et al (1990) to Proc. lVatl. Acad. Sci. USA 87, 3410-3414). In the first of these methods a polycation-antibody complex is formed with the DNA construct or other genetic construct of the invention, wherein the antibody is specific for either wild-type adenovirus or a variant adenovirus in which a new epitope has been introduced which binds the antibody. The polycation moiety Is binds the DNA via electrostatic interactions with the phosphate backbone.
The adenovirus, because it contains unaltered fibre and penton proteins, is internalized into the cell and carries into the cell with it the DNA construct of the invention. It is preferred if the polycation is polylysine.
2o The DNA may also be delivered by adenovirus wherein it is present within the adenovirus particle, for example, as described below.
In the second of these methods, a high-efficiency nucleic acid delivery system that uses receptor-mediated endocytosis to carry DNA
2s macromolecules into cells is employed. This is accomplished by conjugating the iron-transport protein transferrin to polycations that bind nucleic acids. Human transferrin, or the chicken homologue conalbumin, or combinations thereof is covalently linked to the small DNA-binding protein protamine or to polylysines of various sizes through a disulfide linkage. These modified transferrin molecules maintain their ability to bind their cognate receptor and to mediate efficient iron transport into the cell. The transferrin-polycation molecules form electrophoretically stable complexes with DNA constructs or other genetic constructs of the s invention independent of nucleic acid size (from short oligonucleotides to DNA of 21 kilobase pairs). When complexes of transferrin-polycation and the DNA constructs or other genetic constructs of the invention are supplied to the tumour cells, a high level of expression from the construct in the cells is expected.
to High-efficiency receptor-mediated delivery of the DNA constructs or other genetic constructs of the invention using the endosome-disruption activity of defective or chemically inactivated adenovirus particles produced by the methods of Cotten et al (1992) Proc. Natl. Acad. Sci. USA 89, 6094-6098 is may also be used. This approach appears to rely on the fact that adenoviruses are adapted to allow release of their DNA from an endosome without passage through the lysosome, and in the presence of, for example transferrin linked to the DNA construct or other genetic construct of the invention, the construct is taken up by the cell by the same route as the 2o adenovirus particle.
This approach has the advantages that there is no need to use complex retroviral constructs; there is no permanent modification of the genome as occurs with retroviral infection; and the targeted expression system is 2s coupled with a targeted delivery system, thus reducing toxicity to other cell types.
Tt may be desirable to locally perfuse a tumour with the suitable delivery vehicle comprising the genetic construct for a period of time; additionally or alternatively the delivery vehicle or genetic construct can be injected directly into accessible tumours.
It will be appreciated that "naked DNA" and DNA complexed with s cationic and neutral lipids may also be useful in introducing the DNA of the invention into cells of the patient to be treated. Non-viral approaches to gene therapy are described in Ledley (1995) Hufnan Gene Therapy 6, 1129-1144.
to Alternative targeted delivery systems are also known such as the modi~xed adenovirus system described in WO 94/10323 wherein, typically, the DNA is carried within the adenovirus, or adenovirus-like, particle.
Michael et al (1995) Gene Therapy 2, 660-668 describes modification of adenovirus to add a cell-selective moiety into a fibre protein. Mutant is adenoviruses which replicate selectively in p53-deficient human tumour cells, such as those described in Bischoff et al (I996) Science 274, 373-376 are also useful for delivering the genetic construct of the invention to a cell. Thus, it will be appreciated that a further aspect of the invention provides a virus or virus-like particle comprising a genetic construct of the 2o invention. Other suitable viruses or virus-like particles include HSV, AAV, vaccinia and parvovirus.
In a further embodiment the agent which selectively prevents the function of the said VGSC is a ribozyme capable of cleaving targeted VGSC RNA
2s or DNA. A gene expressing said ribozyme may be administered in substantially the same way and using substantially the same vehicles as for the antisense molecules.

Ribozymes which may be encoded in the genomes of the viruses or virus-like particles herein disclosed are described in Cech and Herschlag "Site-specific cleavage of single stranded DNA" US 5,180,818; Altman et al "Cleavage of targeted RNA by RNAse P" US 5,168,053, Cantin et al s "Ribozyme cleavage of HIV-1 RNA" US 5,149,796; Cech et al "RNA
ribozyme restriction endoribonucleases and methods", US 5,116,742;
Been et al "RNA ribozyme polymerases, dephosphorylases, restriction endonucleases and methods", US 5,093,246; and Been et al "RNA
ribozyme polymerases, dephosphorylases, restriction endoribonucleases to and methods; cleaves single-stranded RNA at specific site by transesterification", US 4,987,071, all incorporated herein by reference.
It will be appreciated that it may be desirable that the antisense molecule or ribozyme is expressed from a breast cell-specific promoter element.
is Examples of breast cell-specific promoters include the promoter element for c-erbB2 or the oestrogen receptor.
The genetic constructs of the invention can be prepared using methods well known in the art.
A variety of methods have been developed to operably link DNA to vectors ~ via complementary cohesive termini. For instance, complementary homopolymer tracts can be added to the DNA segment to be inserted to the vector DNA. The vector and DNA segment are then 2s joined by hydrogen bonding between the complementary homopolymeric tails to form recombinant DNA molecules.
Synthetic linkers containing one or more restriction sites provide an alternative method of joining the DNA segment to vectors. The DNA

segment, generated by endonuclease restriction digestion as described earlier, is treated with bacteriophage T4 DNA polymerase or E. coli DNA
polymerase I, enzymes that remove protruding, 3'-single-stranded termini with their 3'-5'-exonucleolytic activities, and fill in recessed 3'-ends with s their polymerizing activities.
The combination of these activities therefore generates blunt-ended DNA
segments. The blunt-ended segments are then incubated with a large molar excess of linker molecules in the presence of. an enzyme that is able to to catalyze the ligation of blunt-ended DNA molecules, such as bacteriophage T4 DNA ligase. Thus, the products of the reaction are DNA segments carrying polymeric linker sequences at their ends. These DNA segments are then cleaved with the appropriate restriction enzyme and ligated to an expression vector that has been cleaved with an enzyme Is that produces termini compatible with those of the DNA segment.
Synthetic linkers containing a variety of restriction endonuclease sites are con>lnercially available from a number of sources including International Biotechnologies Inc, New Haven, CN, USA.
A desirable way to modify the DNA encoding the polypeptide of the invention is to use the polymerase chain reaction as disclosed by Saiki et al (1988) Science 239, 487-491.
2s In this method the DNA to be enzymatically amplified is flanked by two specific oligonucleotide primers which themselves become incorporated into the amplified DNA. The said specific primers may contain restriction endonuclease recognition sites which can be used for cloning into expression vectors using methods known in the art.

The present invention also relates to a host cell transformed with a genetic (preferably DNA construct) construct of the present invention. The host cell can be either prokaryotic or eukaryotic. Bacterial cells are preferred s prokaryotic host cells and typically are a strain of E. coli such as, fox example, the E. coli strains DHS available from Bethesda Research Laboratories Inc., Bethesda, MD, USA, and RR1 available from the American Type Culture Collection (ATCC) of Rockville, MD, USA (No ATCC 31343). Preferred eukaryotic host cells include yeast and .
mammalian cells, preferably vertebrate cells such as those from a mouse, rat, monkey or human fibroblastic cell line. Yeast host cells include YPH499, YPH500 and YPH501 which are generally available from Stratagene Cloning Systems, La Jolla, CA 92037, USA. Preferred mammalian host cells include Chinese hamster ovary (CHO) cells is available from the ATCC as CCL61, NIH Swiss mouse embryo cells NIH/3T3 available from the ATCC as CRL 1658, and monkey kidney-derived COS-1 cells available from the ATCC as CRL 1650.
Transformation of appropriate cell hosts with a DNA construct of the 2o present invention is accomplished by well known methods that typically depend on the type of vector used. With regard to transformation of prokaryotic host cells, see, for example, Cohen et al (1972) Proc. Natl.
Acad. Sci. USA 69, 2110 and Sambrook et al (1989) Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring 2s Harbor, NY. Transformation of yeast cells is described in Sherman et al (1986) Methods In Yeast Genetics, A Laboratory MafZUal, Cold Spring Harbor, NY. The method of Beggs (1978) Nature 275, 104-109 is also useful. With regard to vertebrate cells, reagents useful in transfecting such cells, for example calcium phosphate and DEAE-dextran or liposome formulations, are available from Stratagene Cloning Systems, or Life Technologies Inc., Gaithersburg, MD 20877, USA.
Electroporation is also useful for transforming cells and is well known in s the art for transforming yeast cell, bacterial cells and vertebrate cells.
For example, many bacterial species may be transformed by the methods described in Luchansky et al (1988) Mol. Microbiol. 2, 637-646 incorporated herein by reference. The greatest number of transformants is 1o consistently recovered following electroporation of the DNA-cell mixture suspended in 2.5X PEB using 6250V per cm at 25~.FD.
Methods for transformation of yeast by electroporation are disclosed in Becker & Guarente (1990) Methods Ejzzymol. 194, 182.
is Successfully transformed cells, ie cells that contain a DNA construct of the present invention, can be identified by well known techniques. For example, cells resulting from the introduction of an expression construct of the present invention can be grown to produce the molecule as defined 2o in the invention. Cells can be harvested and lysed and their DNA content examined for the presence of the DNA using a method such as that described by Southern (1975) J. Mol. Biol. 98, 503 or Berent et al (1985) Bioteeh. 3, 208. Alternatively, the presence of .the molecule, for example a protein, in the supernatant can be detected using antibodies as described 2s below.
In addition to directly assaying for the presence of recombinant DNA, successful transformation can be confirmed by well known immunological methods when the recombinant DNA is capable of directing the expression of the protein. For example, cells successfully transformed with an expression vector produce proteins displaying appropriate antigenicity.
Samples of cells suspected of being transformed are harvested and assayed for the protein using suitable antibodies.
s Thus, in addition to the transformed host cells themselves, the present invention also contemplates a culture of those cells, preferably a monoclonal (clonally homogeneous) culture, or a culture derived from a monoclonal culture, in a nutrient medium.
to When the genetic construct is a plasmid DNA construct it can be purified.
The DNA construct of the invention is purified from the host cell using well known methods.
is For example, plasmid vector DNA can be prepared on a Iarge scale from cleaved lysates by banding in a CsCl gradient according to the methods of Clewell & Helinski (1970) Bioche~raistry 9, 4428-4440 and Clewell (1972) J. Bacteriol. 110, 667-676. Plasmid DNA extracted in this way can be freed from CsCI by dialysis against sterile, pyrogen-free buffer through 2o Visking tubing or by size-exclusion chromatography.
Alternatively, plasmid DNA may be purified from cleared lysates using ion-exchange chromatography, for example those supplied by Qiagen.
Hydroxyapatite column chromatography may also be used.
2s A further aspect of the invention provides use of an agent which selectively prevents (including inhibits) the function of SCNSA (and optionally also SCN9A) voltage-gated Na+ channel in the manufacture of a medicament for treating cancer, preferably breast cancer. A further aspect of the invention provides use of an agent which selectively prevents the function of a voltage-gated Na+ channel, preferably SCNSA or SCN9A in the manufacture of a medicament for treating breast cancer (including treating a patient with or at risk of breast cancer).
s A further aspect of the invention provides a method of treating a patient with or at risk of cancer (preferably breast cancer) wherein an agent which selectively prevents (including inhibits) the function of SCNSA (and optionally also SCN9A) voltage-gated Na+ channel is administered to the 1o patient. A further aspect of the invention provides a method of treating a patient with or at risk of cancer (preferably breast cancer) wherein an agent which selectively prevents the function of a voltage-gated Na+ channel, preferably SCNSA or SCN9A is administered to the patient.
1s Agents known as anti-arrhythmic and local anaesthetic drugs may selectively prevent or inhibit the function of SCNSA voltage gated Na+
channels, as well known to those skilled in the art. Antiarrhythmic drugs that have been shown to inhibit SCNSA VGSC activity include: naloxone, flecainide, cinnamophilin and acrophyllidine; local anasthetics include 2o pilsicainide and Iidocaine. However, they are not specific blockers of VGSCs, including SCNSA subtype. Such anti-arrhythmic or local anaesthetic drugs may be preferred agents for use in these aspects of the invention.
2s A still further aspect of the invention provides a genetic construct comprising a nucleic acid encoding a molecule capable of preventing the function of SCNSA (and optionally also SCN9A) voltage-gated Na+
channel expressed in a cell.

A further aspect of the invention provides a genetic construct comprising a nucleic acid encoding a molecule capable of preventing the function of a voltage-gated Na+ channel expressed in a cell, preferably SCNSA and/or SCN9A (most preferably SCNSA), wherein expression of said molecule s by the genetic construct is via a breast-selective promoter, or wherein the genetic construct is adapted for selective delivery to a (human) breast cell.
As noted above, the genetic construct may be RNA or DNA. The molecule capable of preventing the function of the said VGSC is 1o conveniently an antisense molecule or a ribozyme as disclosed above.
The genetic constructs are adapted for delivery to a human cell, in particular a cell which is cancerous or in which cancer may occur, and more particularly the genetic construct is adapted for delivery to a breast is cell. The genetic constructs of this aspect of the invention include the viral and non-viral delivery systems described above.
Suitably, the molecule is capable of preventing the function of the said VGSC, for example SCNSA VGSC, such as a ribozyme or antisense 2o molecule, is selectively expressed in a cancer cell. For example, expression of said molecule by the genetic construct may be via a cancer cell- or tissue-selective promoter which, in the case of breast cancer, may be the MUC-1 promoter or any other breast-selective promoter.
2s A further aspect of the invention provides the genetic constructs for use in medicine, preferably for use in treating cancer, still more preferably breast cancer. Thus, the genetic constructs are packaged and presented for use in medicine. A further aspect of the invention provides the use of the said genetic constructs for use in the manufacture of a medicament for the treatment of cancer, preferably breast cancer.
A further aspect of the invention provides a pharmaceutical composition s comprising a genetic construct of the invention and a pharmaceutically acceptable carrier. The carriers must be "acceptable" in the sense of being compatible with the genetic construct of the invention and not deleterious to the recipients thereof. Typically, the carriers will be water or saline which will be sterile and pyrogen free.
For the avoidance of doubt, the genetic constructs of the invention specifically include virus or virus-like particles, but also include constructs suitable use with for non-viral delivery systems.
Is A further aspect of the invention provides a method of identifying a compound which selectively inhibits a SCNSA voltage-gated Na+ channel, the method comprising (a) contacting a test compound with any one of the said voltage-gated Na+ channels and determining whether said compound is inhibitory; (b) contacting the test compound with other voltage-gated 2o Na+ channels and determining whether said compound is inhibitory; and (c) selecting a compound which is substantially inhibitory in (a) but is not substantially inhibitory in (b). The compound may be useful in the treatment of cancer, preferably breast cancer.
25 Typically, a range of compounds, including pharmacological agents with known effects upon voltage-gated Na+ channels, will be screened for their effectiveness in a number of assays. Suitable assay formats include electrophysiological recording from cells in long-term culture as cell-lines and short term culture of cells dissociated from biopsies (see, for example Grimes & Djamgoz (1998) J. Cell Physiol. 175, 50-58);
electrophysiological recording from oocytes functionally expressing recombinant said VGSC following injection of cRNAs (see, for example, Fraser et al (1993) In ElectroplZysiology, A practical approach (D. Willis, s ed) IRL Press); and in vitro (Boyden chamber) invasion assays (see, for example, Grimes et al (1995) FEBS Lett 369, 290-294; and Smith et al (1998) FEBS Lett. 423, 19-24).
The present invention also provides methods in which treatment is targeted to to cancer cells by means of targeting to cells expressing SCNSA VGSC, or in which treatment is targeted to breast cancer cells by means of targeting to cells expressing a VGSC, preferably SCNSA or SCN9A, as noted above in relation to targeting of genetic constructs to such cells. It will be appreciated that targeting to cells expressing a said VGSC may preferably Is be performed in conjunction with another targeting means or method, for example local administration, in order to minimise adverse effects on any normal tissues that express the said VGSC. For example, cardiac tissue expresses SCNSA at high levels.
2o For example, anti-said VGSC antibodies (VGSC-Abs) conjugated with a dye substance may be applied to the cancerous tissue in vivo (eg Yasmuch et al (1993) "Antibody targeted photolysis" Critical Review Revue T7zer. Drug Carrier System 10, 197-252; Pogrebniak et al (1993) "Targetted phototherapy with sensitizes-monoclonal antibody conjugate and light"
2s Surgical Onoclogy 2, 31-42). The tissue is then irradiated locally with a wavelength of lightllaser matching the absorption peak of the 'attached' dye.
Absorption of the light energy by the dye leads to local heating and cell death. In this way, only the labelled (ie metastatic) cells will be ablated.
VGSC-Abs labelled with the following dyes may be used: fluorescein (Pelegrin et al (1991) "Antibody fluorescein conjugates for photoimmunodiagnosis of human colon-carcinoma in nude-mice" Cajaeer 67, 2529-2537); rhodamine (Haghighat et al (1992) "Laser-dyes for experimental phototherapy of human cancer - comparison of 3 rhodamines"
s Laryngoscope 102, 81-87); cyanins (Folli et al (1994) "Antibody-indocyanin conjugates for immunophotodetection of human squamous-cell carcinoma in nude-mice" Cancer Research 54, 2643-2649; Lipshutz et al (1994) "Evaluation of 4 new carbocyanine dyes for photodynamic therapy with lasers" Laryngoscope 104, 996-1002; Haddad et al (1998) "In vitro to and in vivo effects of photodynamic therapy on murine malignant melanoma" Annals of Surgzcal Oyacology 5, 241-247). This may be useful with oesophageal and lung cancer.
Thus, a further aspect of the invention provides a compound comprising a is moiety which selectively binds SCNSA voltage-gated Na+channel protein and a further moiety.
By "a moiety which selectively binds SCNSA voltage-gated Na+channel protein" we mean any suitable such moiety which binds the said VGSC
2o but does not substantially bind other molecules, for example other VGSCs, for example SCN9A. The compound comprising the binding moiety is one which preferably, in use, is able to localise to areas of cancerous cells (preferably breast cancerous cells), particularly metastatic cancer cells, but not localise substantially to other areas where there are 2s no cancerous cells.
Preferably the binding moiety is able to bind to the said VGSC with high affinity. For example, the binding constant for the binding of the binding moiety to the said VGSC is preferably between 10-' and 10-1° M.

Typically the binding moiety is an anti-SNCSA antibody. Such antibodies and methods of preparing suitable antibodies are discussed above.
The further moiety may be any further moiety which confers on the s compound a useful property with respect to the treatment or imaging or diagnosis of cancer. In particular, the further moiety is one which is useful in killing or imaging cancer cells, particularly metastatic cancer cells. Preferably, the further moiety is one which is able to kill the cancer cells to which the compound is targeted.
In a preferred embodiment of the invention the further moiety is directly or indirectly cytotoxic. In particular the further moiety is preferably directly or indirectly toxic to cancer cells, particularly metastatic cancer cells.
is By "directly cytotoxic" we include the meaning that the moiety is one which on its own is cytotoxic. By "indirectly cytotoxic" we include the meaning that the moiety is one which, although is not itself cytotoxic, can induce cytotoxicity, for example by its action on a further molecule or by 2o further action on it.
In one embodiment the cytotoxic moiety is a cytotoxic chemotherapeutic agent. Cytotoxic chemotherapeutic agents are well known in the art.
2s Cytotoxic chemotherapeutic agents, such as anticancer agents, include:
alkylating agents including nitrogen mustards such as mechlorethamine (HN~), cyclophosphamide, ifosfamide, melphalan (L-sarcolysin) and chlorambucil; ethylenimines and methylmelamines such as hexamethylmelamine, thiotepa; alkyl sulphonates such as busulfan;

nitrosoureas such as carmustine (BCNU), lomustine (CCNU), semustine (methyl-CCNU) and streptozocin (streptozotocin); and triazenes such as decarbaziue (DTIC; dimethyltriazenoimidazole-carboxamide);
Antimetabolites including folic acid analogues such as methotrexate s (amethopterin); pyrimidine analogues such as fluorouracil (5-fluorouracil;
5-FU), floxuridine (fluorodeoxyuridine; FUdR) and cytarabine (cytosine arabinoside); and purine analogues and related inhibitors such as mercaptopurine (6-mercaptopurine; 6-MP), thioguanine (6-thioguanine; TG) and pentostatin (2-deoxycoformycin). Natural Products including vinca alkaloids such as vinblastine (VLB) and vincristine; epipodophyllotoxins such as etoposide and teniposide; antibiotics such as dactinomycin (actinomycin D), daunorubicin (daunomycin; rubidomycin), doxorubicin, bleomycin, plicamycin (mithramycin) and mitomycin (mitomycin C);
enzymes such as L-asparaginase; and biological response modifiers such as is interferon alphenomes. Miscellaneous agents including platinum coordination complexes such as cisplatin (cis-DDP) and carboplatin;
anthracenedione such as mitoxantrone and anthracycline; substituted urea such as hydroxyurea; methyl hydrazine derivative such as procarbazine (N-methylhydrazine, MIH); and adrenocortical suppressant such as mitotane 20 (o,p-DDD) and aminoglutethimide; taxol and analogues/derivatives; and hormone agonists/antagonists such as flutamide and tamoxifen.
Various of these agents have previously been attached to antibodies and other target site-delivery agents, and so compounds of the invention 2s comprising these agents may readily be made by the person skilled in the art. For example, carbodiimide conjugation (Bauminger & Wilchek (1980) Meth~ds Enzymol. 70, 151-159; incorporated herein by reference) may be used to conjugate a variety of agents, including doxorubicin, to antibodies or peptides.

Carbodiimides comprise a group of compounds that have the geneial formula R-N~C=N-R', where R and R' can be aliphatic or aromatic, and are used for synthesis of peptide bonds. The preparative procedure is s simple, relatively fast, and is carried out under mild conditions.
Carbodiimide compounds attack carboxylic groups to change them into reactive sites for free amino groups.
The water soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl) to carbodiimide (EDC) is particularly useful for conjugating a functional moiety to a binding moiety and may be used to conjugate doxorubicin to tumor homing peptides. The conjugation of doxorubicin and a binding moiety requires the presence of an amino group, which is provided by doxorubicin, and a carboxyl group, which is provided by the binding is moiety such as an antibody or peptide.
In addition to using carbodiimides for the direct formation of peptide bonds, EDC also can be used to prepare active esters such as N-hydroxysuccinimide (NHS) ester. The NHS ester, which binds only to 2o amino groups, then can be used to induce the formation of an amide bond with the single amino group of the doxorubicin. The use of EDC and NHS in combination is commonly used for conjugation in order to increase yield of conjugate formation (Bauminger & Wilchek, supra, 190).
Other methods for conjugating a functional moiety to a binding moiety also can be used. For example, sodium periodate oxidation followed by reductive alkylation of appropriate reactants can be used, as can glutaraldehyde crosslinking. However, it is recognised that, regardless of which method of producing a conjugate of the invention is selected, a determination must be made that the binding moiety maintains its targeting ability and that the functional moiety maintains its relevant function.
s In a further embodiment of the invention, the cytotoxic moiety is a cytotoxic peptide or polypeptide moiety by which we include any moiety which leads to cell death. Cytotoxic peptide and polypeptide moieties are well known in the art and include, for example, ricin, abrin, Pseudomonas exotoxin, tissue factor and the like. Methods for linking them to targeting 1o moieties such as antibodies are also known in the art. The use of ricin as a cytotoxic agent is described in Burrows & Thorpe (1993) Proc. Natl.
Aead. Sci. Z7SA 90, 8996-9000, incorporated herein by reference, and the use of tissue factor, which leads to localised blood clotting and infarction of a tumour, has been described by Ran et al (1998) Cancer Res. 58, is 4646-4653 and Huang et al (1997) Science 275, 547-550. Tsai et al (1995) Dis. Colon Rectum 38, 1067-1074 describes the abrin A chain conjugated to a monoclonal antibody and is incorporated herein by reference. Other ribosome inactivating proteins are described as cytotoxic agents in WO 96/06641. Pseudomonas exotoxin may also be used as the 2o cytotoxic polypeptide moiety (see, for example, Aiello et al (1995) Proc.
Natl. Acad. Sci. LISA 92, 10457-10461; incorporated herein by reference).
Certain cytokines, such as TNFa and IL-2, may also be useful as cytotoxic agents.
2s Certain radioactive atoms may also be cytotoxic if delivered in sufficient doses. Thus, the cytotoxic moiety may comprise a radioactive atom which, in use, delivers a sufficient quantity of radioactivity to the target site so as to be cytotoxic. Suitable radioactive atoms include phosphorus-32, iodine-125, iodine-131, indium-111, rhenium-186, rhenium-188 or yttrium-90, or any other isotope which emits enough energy to destroy neighbouring cells, organelles or nucleic acid. Preferably, the isotopes and density of radioactive atoms in the compound of the invention are s such that a dose of more than 4000 cGy (preferably at least 6000, 8000 or 10000 cGy) is delivered to the target site and, preferably, to the cells at the target site and their organelles, particularly the nucleus.
The radioactive atom may be attached to the binding moiety in known to ways. For example EDTA or another chelating agent may be attached to the binding moiety and used to attach 111In or 9oY. Tyrosine residues may be labelled with lasl ox 1311.
The cytotoxic moiety may be a suitable indirectly cytotoxic polypeptide.
is In a particularly preferred embodiment, the indirectly cytotoxic polypeptide is a polypeptide which has enzymatic activity and can convert a relatively non-toxic prodrug into a cytotoxic drug. When the targeting moiety is an antibody this type of system is often referred to as ADEPT
(Antibody-Directed Enzyme Prodrug Therapy). The system requires that 2o the targeting moiety locates the enzymatic portion to the desired site in the body of the patient (ie the site expressing the said VGSC, such as metastatic cancer cells) and after allowing time for the enzyme to localise at the site, administering a prodrug which is a substrate for the enzyme, the end product of the catalysis being a cytotoxic compound. The object 2s of the approach is to maximise the concentration of drug at the desired site and to minimise the concentration of drug in normal tissues (see Senter, P.D. et. al (1988) "Anti-tumor effects of antibody-alkaline phosphatase conjugates in combination with etoposide phosphate" Proc. Natl. Acad.
Sci. USA 85, 4842-4846; Bagshawe (1987) Br. J. Cancer 56, 531-2; and Bagshawe, K.D. et al (1988) "A cytotoxic agent can be generated selectively at cancer sites" Br. J. Cancer. 58, 700-703.) Clearly, any said VGSC-binding moiety may be used in place of an anti s said VGSC antibody in this type of directed enzyme prodrug therapy system.
The enzyme and prodrug of the system using a said VGSC-targeted enzyme as described herein may be any of those previously proposed.
to The cytotoxic substance may be any existing anti-cancer drug such as an alkylatiug agent; an agent which intercalates in DNA; an agent which inhibits any key enzymes such as dihydrofolate reductase, thymidine synthetase, ribonucleotide reductase, nucleoside kinases or topoisomerase;
or an agent which effects cell death by interacting with any other cellular is constituent. Etoposide is an example of a topoisomerase inhibitor.
Reported prodrug systems include: a phenol mustard prodrug activated by an E. coli ~3-glucuronidase (Wang et al, 1992 and Roffler et al, 1991); a doxorubicin prodrug activated by a human ~3-glucuronidase (Bosslet et al, 20 1994); further doxorubicin prodrugs activated by coffee bean cx-galactosidase (Azoulay et al, 1995); daunorubicin prodrugs, activated by coffee bean cc-D-galactosidase (Gesson et al, 1994); a 5-fluorouridine prodrug activated by an E. coli [3-D-galactosidase (Abraham et al, 1994);
and methotrexate prodrugs (cg methotrexate-alanine) activated by 2s carboxypeptidase A (Kuefner et al, 1990, Vitols et al, 1992 and Vitols et al, 1995). These and others are included in the following table.

Enzyme Prodrug Carboxypeptidase Derivatives of L-glutamic acid and G2 benzoic acid mustards, aniline mustards, phenol mustards and phenylenediamine mustards;

fluorinated derivatives of these Alkaline phosphataseEtoposide phosphate Mitomycin phosphate Beta-glucuronidasep-Hydroxyaniline mustard-glucuronide Epirubicin-glucuronide Penicillin-V-amidaseAdriamycin-N phenoxyacetyl Penicillin-G-amidaseN-(4'-hydroxyphenyl acetyl) palytoxin Doxorubicin and melphalan Beta-lactamase Nitrogen mustard-cephalosporin p-phenylenediamine; doxorubicin derivatives;

vinblastine derivative-cephalosporin, cephalosporin mustard; a taxol derivative ', Cyanophenylmethyl-beta-D-gluco-Beta-glucosidase I pyranosiduronic acid Nitroreductase 5-(Azaridin-1-yl-)-2,4-dinitrobenzamide Cytosine deaminase5-Fluorocytosine i CarboxypeptidaseMethotrexate-alanine A

(This table is adapted from Bagshawe (1995) Drug Dev. Res. 34, 220-230, from which full references for these various systems may be obtained; the taxol derivative is described in Rodrigues, M.L. et al (1995) Cl~errzistry &
s Biology Z, 223).
Suitable enzymes for forming part of the enzymatic portion of the invention include: exopeptidases, such as carboxypeptidases G, Gl and G2 (for glutamylated mustard prodrugs), carboxypeptidases A and B (for MTX-based prodrugs) and aminopeptidases (for 2-a-aminocyl MTC

prodrugs); endopeptidases, such as eg thrombolysin (for thrombin prodrugs); hydrolases, such as phosphatases (eg alkaline phosphatase) or sulphatases (eg aryl sulphatases) (for phosphylated or sulphated prodrugs);
amidases, such as penicillin amidases and arylacyl amidase; lactamases, s such as ~i-lactamases; glycosidases, such as (3-glucuronidase (for ~i-glucuronomide anthracyclines), a-galactosidase (for amygdalin) and ~i-galactosidase (for (3-galactose anthracycline); deaminases, such as cytosine deaminase (for SFC); kinases, such as urokinase and thymidine kinase (for gancyclovir); reductases, such as nitroreductase (for CB1954 and 1o analogues), azoreductase (for azobenzene mustards) and DT-diaphorase (for CB1954); oxidases, such as glucose oxidase (for glucose), xanthine oxidase (for xanthine) and lactoperoxidase; DL-racemases, catalytic antibodies and cyclodextrins.
is The prodrug is relatively non-toxic compared to the cytotoxic drug.
Typically, it has less than 10 % of the toxicity, preferably less than 1 % of the toxicity as measured in a suitable in vitro cytotoxicity test.
It is likely that the moiety which is able to convert a prodrug to a cytotoxic 2o drug will be active in isolation from the rest of the compound but it is necessary only for it to be active when (a) it is in combination with the rest of the compound and (b) the compound is attached to, adjacent to or internalised in target cells.
2s When each moiety of the compound is a polypeptide, the two portions may be linked together by any of the conventional ways of cross-linking polypeptides, such as those generally described in O'Sullivan et al (1979) Anal. Bioclzem. 100, 100-108. For example, the said VGSC binding moiety may be enriched with thiol groups and the further moiety reacted with a bifunctional agent capable of reacting with those thiol groups, for example the N-hydroxysuccinimide ester of iodoacetic acid (NHIA) or N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP). Amide and thioether bonds, for example achieved with m-maleimidobenzoyl-N-s hydroxysuccinimide ester, are generally more stable in vivo than disulphide bonds.
Alternatively, the compound may be produced as a fusion compound by recombinant DNA techniques whereby a length of DNA comprises to respective regions encoding the two moieties of the compound of the invention either adjacent one another or separated by a region encoding a linker peptide which does not destroy the desired properties of the compound. Conceivably, the two portions of the compound may overlap wholly or partly.
is The DNA is then expressed in a suitable host to produce a polypeptide comprising the compound of the invention.
The cytotoxic moiety may be a radiosensitizer. Radiosensitizers include 2o fluoropyrimidines, thymidine analogues, hydroxyurea, gemcitabine, fludarabine, nicotinamide, halogenated pyrimidines, 3-aminobenzamide, 3-aminobenzodiamide, etanixadole, pimonidazole and misonidazole (see, for example, McGinn et al (1996) J. Natl. Caracer Inst. 88, 1193-11203;
Shewach & Lawrence (1996) Ifavest. New Drugs 14, 257-263; Horsman 2s (1995) Acta Oncol. 34, 571-587; Shenoy & Singh (1992) Clin. Invest. 10, 533-SS1; Mitchell et al (1989) Ijat. J. Radiat. Biol. 56, 827-836; Iliakis &
I~urtzman (1989) Int. J. Radial. Oncol. Biol. Phys. 16, 1235-1241; Brown (1989) Int. J. Radiat. OjZCOl. Biol. Phys. 16, 987-993; Brown (1985) Cancer 55, 2222-2228).

Also, delivery of genes into cells can radiosensitise them, for example delivery of the p53 gene or cyclin D (Lang et al (1998) J. Neurosurg. 89, 125-132; Coco Martin et al (1999) Cancer Res. 59, 1134-1140).
s The further moiety may be one which becomes cytotoxic, or releases a cytotoxic moiety, upon irradiation. For example, the boron-10 isotope, when appropriately irradiated, releases a, particles which are cytotoxic (see for example, US 4, 348, 376 to Goldenberg; Primus et al (1996) to Bioconjug. Chem. 7, 532-535).
Similarly, the cytotoxic moiety may be one which is useful in photodynamic therapy such as photofrin (see, for example, Dougherty et al (1998) J. Natl. Cancer Inst. 90, 889-905).
is The further moiety may comprise a nucleic acid molecule which is directly or indirectly cytotoxic. For example, the nucleic acid molecule may be an antisense oligonucleotide which, upon localisation at the target site is able to enter cells and lead to their death. The oligonucleotide, therefore, may 2o be one which prevents expression of an essential gene, or one which leads to a change in gene expression which causes apoptosis.
Examples of suitable oligonucleotides include those directed at bcl-2 (Ziegler et al (1997) J. Natl. Cancer Inst. 89, 1027-1036), and DNA
2s polymerase a and topoisomerase IIa (Lee et al (1996) Anticancer Res. 16, 1805-1811.
Peptide nucleic acids may be useful in place of conventional nucleic acids (see Knudsen & Nielsen (1997) Anticancer Drugs 8, 113-118).

In a further embodiment, the binding moiety may be comprised in a delivery vehicle for delivering nucleic acid to the target, for example a nucleic acid as discussed in relation to earlier aspects of the invention.
s The delivery vehicle may be any suitable delivery vehicle. It may, for example, be a liposome containing nucleic acid, or it may be a virus or virus-life particle which is able to deliver nucleic acid. In these cases, the moiety which selectively binds to the said VGSC is typically present on the surface of the delivery vehicle. For example, the moiety which Zo selectively binds to the said VGSC, such as a suitable antibody fragment, may be present in the outer surface of a liposome and the nucleic acid to be delivered may be present in the interior of the liposome. As another example, a viral vector, such as a retroviral or adenoviral vector, is engineered so that the moiety which selectively binds to the said VGSC is is attached to or located in the surface of the viral particle thus enabling the viral particle to be targeted to the desired site. Targeted delivery systems are also known, such as the modified adenovirus system discussed above.
Immunoliposomes (antibody-directed liposomes) may be used in which the 2o moiety which selectively binds to the said VGSC is an antibody. The preparation of immunoliposomes is described above.
The nucleic acid delivered to the target site may be any suitable DNA
which leads, directly or indirectly, to cytotoxicity. For example, the 2s nucleic acid may encode a ribozyme which is cytotoxic to the cell, or it may encode an enzyme which is able to convert a substantially non-toxic prodrug into a cytotoxic drug (this latter system is sometime called GDEPT: Gene Directed Enzyme Prodrug Therapy).

Ribozymes which may be encoded in the nucleic acid to be delivered to the target are described in references cited above. Suitable targets for ribozymes include transcription factors such as c-fos and c-myc, and bcl 2. Durai et al (1997) Anticancer Res. 17, 3307-3312 describes a s hammerhead ribozyme against bcl-2.
EP 0 415 731 describes the GDEPT system. Similar considerations concerning the choice of enzyme and prodrug apply to the GDEPT system as to the ADEPT system described above.
The nucleic acid delivered to the target site may encode a directly cytotoxic polypeptide.
In a further embodiment of the invention, the further moiety comprised in Is the compound of the invention is a readily detectable moiety.
By a "readily detectable moiety" we include the meaning that the moiety is one which, when located at the target site following administration of the compound of the invention into a patient, may be detected, typically 2o non-invasively from outside the body and the site of the target located.
Thus, the compounds of this embodiment of the invention are useful in imaging and diagnosis.
Typically, the readily detectable moiety is or comprises a radioactive atom 2s which is useful in imaging. Suitable radioactive atoms include technetium-99m or iodine-123 for scinitgraphic studies. Other readily detectable moieties include, for example, spin labels for magnetic resonance imaging (MRI) such as iodine-123 again, iodine-131, indium-111, fluorine-19, carbon-13, nitrogen-15, oxygen-17, gadolinium, manganese or iron. Clearly, the compound of the invention must have sufficient of the appropriate atomic isotopes in order for the molecule to be readily detectable.
s The radio- or other labels may be incorporated in the compound of the invention in known ways. For example, if the binding moiety is a polypeptide it may be biosynthesized or may be synthesized by chemical amino acid synthesis using suitable amino acid precursors involving, for example, fluorine-19 in place of hydrogen. Labels such aS 99mTc, 1231, l0 186Rh, 188Rh and 111In can, for example, be attached via cysteine residues in the binding moiety. Yttrium-90 can be attached via a lysine residue.
The IODOOEN method (Fraker er al (1978) Biochem. Biophys. Res.
Comm. 80, 49-57) can be used to incorporate iodine-123. Reference ("Monoclonal Antibodies in Immunoscintigraphy", J-F Chatal, CRC
is Press, 1989) describes other methods in detail.
In a further preferred embodiment of the invention the further moiety is able to bind selectively to a directly or indirectly cytotoxic moiety or to a readily detectable moiety. Thus, in this embodiment, the further moiety 2o may be any moiety which binds to a further compound or component which is cytotoxic or readily detectable.
The further moiety may, therefore be an antibody which selectively binds to the further compound or component, or it may be some other binding 2s moiety such as streptavidin or biotin or the like. The following examples illustrate the types of molecules that are included in the invention; other such molecules are readily apparent from the teachings herein.

The further moiety may be or comprise a bispeciflc antibody wherein one binding site comprises the moiety which selectively binds to the said VGSC and the second binding site comprises a moiety which binds to, for example, an enzyme which is able to convert a substantially non-toxic s prodrug to a cytotoxic drug.
The compound may be an antibody which selectively binds to the said VGSC, to which is bound biotin. Avidin or streptavidin which has been labelled with a readily detectable label may be used in conjunction with to the biotin labelled antibody in a two-phase imaging system wherein the biotin labelled antibody is first localised to the target site in the patient, and then the labelled avidin or streptavidin is administered to the patient.
Bispecific antibodies and biotin/streptavidin (avidin) systems are reviewed by Rosebrough (1996) Q J Nucl. Med. 40, 234-251.
is In a preferred embodiment of the invention, the moiety which selectively binds to the said VGSC and the further moiety are polypeptides which are fused.
2o A further aspect of the invention comprises a nucleic acid molecule encoding a compound of the preceding aspect of the invention.
A further aspect of the invention provides a compound of the invention for use in medicine. Typically, the compound is packaged and presented as a 2s medicament or as an imaging agent or as a diagnostic agent for use in a patient.

A still further aspect of the invention provides a pharmaceutical composition comprising a compound according to the invention and a pharmaceutically acceptable carrier.
Typically the pharmaceutical compositions or formulations of the invention are for parenteral administration, more particularly for intravenous administration.
Formulations suitable for parenteral administration include aqueous and to non-aqueous sterile injection solutions which may contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which may include suspending agents and thickening agents.
is A still further aspect of the invention provides the use of a compound of the invention in the manufacture of a medicament for the treatment and/or diagnosis of a human patient with or at risk of cancer, preferably breast cancer.
A still further aspect of the invention comprises a method of treating cancer (preferably breast cancer) the method comprising administering to the human patient an effective amount of a compound of the invention wherein the further moiety of the compound is one which either directly 2s or indirectly is of therapeutic benefit to the patient.
A still further aspect of the invention comprises a method of imaging cancer, preferably breast cancer, (which may be useful in determining the susceptibility of a human patient to cancer, or of diagnosing cancer in a human patient, or of predicting the relative prospects of a particular outcome of a cancer) in a human patient, comprising administering to the patient an effective amount of a compound of the invention wherein the further moiety of the compound is one which comprises a readily s detectable moiety.
A still further aspect of the invention provides the use of a compound comprising a moiety which selectively binds a voltage-gated Na+channel protein, preferably SCNSA or SCN9A, most preferably SCNSA, and a to further moiety (as defined above) in the manufacture of a medicament for the treatment and/or diagnosis of a human patient with or at risk of breast cancer.
A still further aspect of the invention comprises a method of treating is breast cancer, the method comprising administering to the human patient an effective amount of a compound comprising a moiety which selectively binds a voltage-gated Na+channel protein, preferably SCNSA or SCN9A, most preferably SCNSA, and a further moiety (as defined above) wherein the further moiety of the compound is one which either directly or 2o indirectly is of therapeutic benefit to the patient.
A still further aspect of the invention comprises a method of imaging breast cancer in a human patient, comprising administering to the patient an effective amount of a compound comprising a moiety which selectively 2s binds a voltage-gated Na+channel protein, preferably SCNSA or SCN9A, most preferably SCNSA, and a further moiety (as defined above) wherein the further moiety of the compound is one which comprises a readily detectable moiety.

It will readily be appreciated that, depending on the particular compound used in treatment, imaging or diagnosis, the timing of administration may vary and the number of other components used in therapeutic systems disclosed herein may vary.
s For example, in the case where the compound (for example compound of the invention) . comprises a readily detectable moiety or a directly cytotoxic moiety, it may be that only the compound, in a suitable formulation, is administered to the patient. Of course, other agents such as to immunosuppressive agents and the like may be administered.
In respect of compounds which are detectably labelled, imaging takes place once the compound has localised at the target site.
is However, if the compound is one which requires a further component in order to be useful for treatment, imaging or diagnosis, the compound of the invention may be administered and allowed to localise at the target site, and then the further component administered at a suitable time thereafter.
For example, in respect of the ADEPT and ADEPT-like systems above, the binding moiety-enzyme moiety compound is administered and localises to the target site. Once this is done, the prodrug is administered.
2s Similarly, for example, in respect of the compounds wherein the further moiety comprised in the compound is one which binds a further component, the compound may be administered first and allowed to localise at the target site, and subsequently the further component is administered.

Thus, in one embodiment a biotin-labelled anti-SNCSA antibody (for example) is administered to the patient and, after a suitable period of time, detectably labelled streptavidin is administered. Once the streptavidin has localised to the sites where the antibody has localised (ie the target sites) imaging takes place.
It is believed that the compounds of the invention wherein the further moiety is a readily detectable moiety may be useful in determining the to metastatic state of cancer cells. This may be an important factor influencing the nature and outcome of future therapy.
A further aspect of the invention provides a kit of parts (or a therapeutic system) comprising (1) a compound of the invention wherein the further i5 moiety is a cytotoxic moiety which is able to convert a relatively non-toxic prodrug into a cytotoxic drug and (2) a relatively non-toxic prodrug. The kit of parts may comprise any of the compounds of the invention and appropriate prodrugs as herein described.
2o A still further aspect of the invention provides a kit of parts (or a therapeutic system) comprising (1) a compound of the invention wherein the further moiety is able to bind selectively to a directly or indirectly cytotoxic moiety or to a readily detectable moiety and (2) any one of a directly or indirectly cytotoxic moiety or a readily detectable moiety to 25 which the further moiety of the compound is able to bind.
For example, a kit of parts may contain an anti-SNCSA antibody labelled with biotin and streptavidin labelled with a readily detectable label as defined above.

The invention will now be described by reference to the following, non-Iimiting Example and Figures.
s Figure I. Voltage-gated membrane currents recorded in (A) MDA-MB-231 cells and (B) MCF-7 cells (B). The currents were generated by pulsing the membrane potential from a holding voltage of -100 mV, in 10 mV steps, to +60 mV for 40 ms (A) and 200 ms (B), respectively.
Voltage pulses (indicated by the arrow-heads) were applied with a repeat to interval of 20 s. Only every second current trace generated is displayed.
(C) A typical current-voltage (I-V) relationship generated in MDA-MB-231 cells by pulsing the membrane potential from a holding voltage of -100 mV to test potentials between -70 to +70 mV in 5 mV increments.
Voltage pulses were applied with a repeat interval of 20 s.
is Figure 2,. Suppression of the inward current in MDA-MB-231 cells by tetrodotoxin (TTX). (A) A typical recording showing the effect of I ~.M
TTX; the suppression effect, which was partial, was fully reversible. The currents were generated by pulsing the membrane potential from a holding 2o voltage of -mV to -10 mV for 40 ms. Voltage pulses (indicated by the arrow-head) were applied with a repeat interval of 20 s. The effect of TTX shown resulted from the fourth pulse following drug application. (B) TTX dose-response curve for MDA-MB-231 cells. Cells were depolarised from -100 mV to -10 mV for 40 ms with a repeat interval of 20 s. The 2s percentage reduction of the peak current at the fourth pulse following TTX
application, was plotted as a function of drug concentration. Each point represents the mean of > 5 different cells; error bars denote standard errors. Dotted line denotes 0 % reduction.

Figure 3. SQT-PCR electrophoresis results for scn5a (A), scn8a (B), scn9a (C) and hCytbSR (D). Representative PCR cycle numbers for given bands are indicated above the gels. In each panel, the top image was s derived from MDA-MB-231 cell extracts; the bottom image, from MCF-7 extracts, as indicated in (A).
Figure 4. Electrophoresis results of scn5a (C), scn8a (D), scn9a (E) and hCytbSR (F) RT-PCRs performed on breast cancer tissue samples. Sample to numbers and associated evident lymph node metastasis (LNM) are indicated above the gel images (A and B). Multiple bands corresponding to the evident splice form products (previously described in reference [21]) are shown to the left. PCRs were performed for 55, 40, 40 and 30 cycles for scn5a, scn8a, scn9a and hCytbSR tests, respectively, except for is samples 5 (scn8a and scn9a, 50 cycles each) and 6 (hCytbSR, 40 cycles).
(+) indicates evident LNM, (-) indicates that LNM was not clinically evident. NT = not tested.
Fi, gure 5. Proposed relative (%) expression levels of the three VGSCas 2o found to occur in the strongly (white bars) and weakly (black bars) metastatic cell Lines. In each case, the vertical axis denotes the approximate level of expression with respect to total levels of expression of these three VGSCas in the strongly metastatic MDA-MB-231 cells.
Relative expression levels were estimated from degenerate screens and 2s SQT-PCR data, taken together.
Example 1: Upregulation of voltage-gated Nab' channel expression and metastatic potential in human breast cancer: Correlative studies on cell lines and biopsy tissues Voltage-gated Na+ channel (VGSC) expression in human breast cancer cell lines and breast cancer tissues was studied by electrophysiological and reverse-transcription polymerase chain reaction (RT-PCR) methods in a s correlative approach. Whole-cell patch-clamp recordings revealed depolarisation-activated Na+ currents in 29 % of the strongly metastatic MDA-MB-231 cell line, but never in the weakly metastatic MCF-7 cells.
These currents were largely tetrodotoxin (TTX)-resistant. The expression of three VGSC a subunit (VGSCa) genes, SCNSA, SCN8A and SCN9A
to was determined in both cell lines. Two of these genes (SCNSA and SCN9A) were found to be more highly expressed in the MDA-MB-231 cells, with semi-quantitative RT-PCRs indicating the relative levels of expression as: scn5a > > scn9a > scn8a. The predominant increase in the expression of scn5a (~1~00-fold), also termed hl or SkM2, which is indeed yields TTX-resistant VGSCs, was largely responsible for the greater level of VGSCa expression in the strongly metastatic cells. RT-PCRs performed on breast cancer tissues in a double-blind test showed a strong correlation between the detection of SCNSA gene products and clinically assessed lymph node metastasis. Thus, all biopsies with evident 20 lymph node metastases expressed scn5a; the reverse situation was also mainly true. We conclude that VGSC upregulation occurs as an integral part of the metastatic process in breast cancer, as in prostate cancer, and could serve as a novel marker of the metastatic phenotype.
25 Background Breast cancer is the third most common cancer world wide and the most common cancer of women, affecting 1 in ~ in the western world (1,2). In the USA, breast cancer is the second leading cause of female cancer mortality accounting for about 10 % of all cancer .deaths (3). In breast cancer, as in other cancers, metastasis is the main cause of death in most patients. To date, several breast cancer metastasis-associated genes have been identified (for review, see reference 2). However, indirect measures of metastatic progression, including assessment of intratumoral vascular s invasion, presence or absence of lymph node involvement and size of the primary carcinoma remain the most, widely used methods for the assessment of breast cancer progression. Electro-diagnosis has also been practised, although its cellular/molecular basis remains unknown (4).
to We have shown previously that the functional expression of a voltage-gated Na+ channel (VGSC) can distinguish strongly and weakly metastatic human and rat prostatic cancer cells (5,6) and that VGSC
activity contributes to cellular behaviours integral to metastasis, including cellular process extension (7), lateral motility (8), transverse invasion Is (5,6,9) and secretory membrane activity (10). Carcinomas of the breast and prostate have some similar features, including hormone-sensitivity, a pronounced tropism for metastasis to bone and tendency for co-occurrence in families (11).
2o Voltage-gated Nay channels (VGSCs)1 are composed of a large (~ 240 kD), four-transmembrane domain cc-subunit (VGSCa) and several different auxillary ~3-subunits (VGSC~is) (Catterall, W.A. (1986) Ann.
Rev. Biochem. 55, 953-985). Expression of the VGSCoc alone is sufficient for functional channel formation (Goldin, A. L et al (1986). Proc. Natl.
2s Acad. Sci USA 83, 7503-7507). The VGSC~3(s) serve a number of supporting roles such as facilitating functional channel availability (Isom, L.L., et al (1995) Cell 83, 433-442), modulating channel kinetics (Isom, L.L., et al (1992) Science 256, 839-842, Cannon, S.C., et al (1993) Pflugers Arch. 423, 155-157) and perhaps even altering pharmacological characteristics (Bonhaus, D.W., et al (1996) Neuropharmacol. 35, 605-613) (see also (15)).
VGSCas constitute a family of at least twelve different genes in higher s vertebrates (Plummer, N.W. and Meisler, M.H. (1998) Genomics 57, 323-331; 17), denoted SCN1A to SCN11A; their products have been cloned from a variety of excitable cell types. Their specific expressions are under dynamic, spatio-temporal control. At least two subfamilies of VGSCa genes have been described based on sequence data: Navl and to Nav2 (George, A.L., et al (1992) Proc. Natl. Acad. Sci. USA 89, 4893-4897). Although not yet experimentally determined, it is generally held that these subfamilies represent VGSCas with markedly different electro-physiological properties (Akopian, A.N., et al (1997) FEBS Letts. 400, 183-187). In fact the lack of conservation of landmark VGSCa sequences is in Nav2 VGSCas implies that they may not even be voltage-gated or Na+
selective (Akopian, A.N., et al (1997) FEBS Letts. 400, 183-187, Schlief, T., et al (1996) Eur. Biophys. J. 25, 75-91). The existence of a third subfamily, Nav3, has recently been proposed with the cloning of a cDNA
(NaN/SNS2) from rat dorsal root ganglion (DRG) cells. Although 2o NaN/SNS2 shares less than 50 % sequence homology with other VGSCas, its deduced amino acid sequence possesses all the characteristic sequences of Navl VGSCas (Dib-Hajj, S.D, et al (1998) Proc. Natl. Acad. Sci.
USA 95, 8963-8968).
2s By utilizing RT-PCR and ira situ hybridization methods, several studies have documented the simultaneous expression of multiple VGSCas within diverse cell types (Black, J.A., et al (2994) Mol. Brain Res. 23, 235-245;

Dib-Hajj, S.D., et al (1996) FEBS Letts. 384, 78-82; Fjell, J., et al (1999) Mol. Brain Res. 67, 267-282). Particular VGSCas have been found to be expressed at different levels, with expression under dynamic control (e.g. during development or injury). For example, mRNAs for at s least eight different VGSCas were found in adult rat DRG cells, with a wide range of expression levels: RB1, Na6, NaN/SNS2 and SCL-11 mRNAs were expressed at very high levels, PN1 and SNS/PN3 at intermediate levels, and RB2 and RB3 at very low levels (Dib-Hajj, S.D, et al (1998) Proc. Natl. Acad. Sci. USA 95, 8963-8968; Black, J.A., et to al (1996) Molec. Brain Res. 43, 117-131; Sangameswaren, L., et al (1997) J. Biol. Chem. 272, 14805-14809). Following axonal injury SNS
and NaN/SNS2 mRNAs were dramatically down-regulated, whilst expression of RBl, RB2 and RB3 was up-regulated (Dib-Hajj, S., et al (1996) Proc. Natl. Acad. Sci. USA 93, 14950-14954; Dib-Hajj, S.D., et is al (1998) J. Neurophysiology 79, 2668-2676).
VGSCa genes can occur as a number of alternatively spliced isoforms, expression of which is also under dynamic control. Alternative splicing of exons coding for the third segment (S3) of the first transmembrane domain 20 (D1) has been found to be developmentally regulated for SCN2A and SCN3A (19, 20), yielding "neonatal" and "adult" forms. These code for proteins which differ by only one amino acid, positioned at the extreme extracellular end of S3. The effect of this change on VGSCa function is presently unclear. Similar alternatively spliced exons exist at the 2s corresponding position in SCNBA and SCN9A (Belcher, S.M et al (1995) Proc. Natl. Acad. Sci. USA 92, 11034-11038; Plummer, N.W., et al (1998) Genomics 54, 287-296) but not in SCN4A, SCNSA, SCN10A and SCN11A (George, A.L., et al (1993) Genomics 15, 598-606; Wang, D.W., et al (1996) Biophys. J. 70, 238-245; Souslova, V.A., et al (1997) Genomics 41, 201-209; Dib-Hajj, S.D., et al (1999) Genomics 59, 309-318). To date, no evidence of such alternative splicing has been found for SCN1A or SCN7A. Alternative splicing also occurs in other regions of the VGSCoc, particularly inter-domain (ID) 1-2 and D3.
s The strict regulation of multiple VGSCa gene and splice product expression within the available VGSCa mRNA pool, among different tissue types and during development or following injury (e.g. Dib-Hajj, S., et al (1996) Proc. Natl. Acad. Sci. USA 93, 14950-14954; Dib-Hajj, S.D., et al (1998) J. Neurophysiology 79, 2668-2676; Kallen, R.G., et al (1990) Neuron 4, 233-242) would suggest that different VGSCa gene products and their isoforms are likely to have significantly different functional roles, which, at present, are largely unknown.
Is The functional roles of VGSCs are best understood in the central nervous system where VGSC activity controls not only basic impulse generation and conduction but also directional and patterned growth, including target-specific axonal migration and regional synaptic connectivity (Catalano, S.M. and Shatz, C.J. (1998) Science 281, 559-562; Penn, A.A., et al 20 (1998) Science 279, 2108-2112; Shatz, C.J. (1990) Neuron 5, 74S-756).
VGSCs have also been implicated in several hereditary diseases of excitable tissues (Plummer, N.W. and Meisler, M.H. (1998) Genomics 57, 323-331; Zhou, J. and Hoffman, E.P. (1994) J. Biol. Chem. 269, 18563-18571), and in more complicated pathological disorders, including 2s chronic pain syndromes (Tanaka, M., et al (1998) NeuroReport 9, 967-972), epilepsy (Bartolomei, F., et al (1997) J.Neurocytol. 26, 667-678), ischaemic stroke (Skaper, S.D., et al (1998) FASEB J. 12, 725-731) and Alzheimer's disease (Kanazirska, M., et al (1997) Biochem. Biophys.
Res. Comm. 232, 84-87). There is increasing evidence that VGSC

expression is also associated with strong metastatic potential in rat (MAT-LyLu) and human (PC-3) models of prostate cancer (Grimes, J.A. , et al (1995) FEBS Letts. 369, 290-294; Laniado, M., et al (1997) Am. J.
Pathol. 150, 1213-1221; Smith, P., et al (1998) FEBS Letts 423, 19-24).
s Indeed, expression of functional VGSCs may have a direct, positive influence upon the metastatic process. Accordingly, blockage of VGS
currents in these strongly metastatic cell lines, by application of tetrodotoxin (TTX), significantly (~30 %) reduced the cells' invasive potential. Electro-physiological and pharmacological properties of the to current in the rat were consistent with the channels being neuronal, TTX-sensitive (Navl) type (Grimes, J.A. and Djamgoz, M.B.A. (1998) J. Cell.
Physiol. 175, 50-58). SCN4A gene expression was found in both strongly and weakly metastatic cell lines of human and rat (Diss, J.K.J., et al (1998) FEBS Letts 427, 5-IO). However, the pharmacological properties ?s of the VGS currents in the rat MAT-LyLu cells were not consistent with those reported for this VGSCa. This could result from (1) the numerous differences determined in the MAT-LyLu/AT-2 rSkMl primary sequence;
(2) differences in post-translational mechanisms (cg association with auxiliary subunits, level of glycosylationl phosphorylation of the channel) 2o in these cells; or (3) the presence of other VGSCas in the MAT-LyLu cells that produce the recorded VGS currents.
The present study aimed to determine (t) whether mRNA and functional protein expression of VGSCs differed between strongly and weakly 2s metastatic breast cancer cells; (ii) which members) of the VGSCa, family was responsible for the voltage-gated Na+ (VGS) currents detected; and (iii) whether the VGSCa, expression pattern found in in vitro models would also be reflected in human breast cancer biopsy tissues. These aspects were studied using electrophysiolo,gical and reverse-transcription polymerase chain reaction (RT-PCR) based techniques. Initially, two robust breast cancer cell lines of contrasting metastatic aggressiveness were adopted: the strongly metastatic MDA-MB-231 cells and the weakly metastatic MCF-7 cells (18,19). The mRNA(s) responsible for the s functional VGSC a-subunit expression was determined. Finally, VGSC
mRNA expression was also investigated in frozen biopsy tissues of different clinical grade to test whether VGSCa occurrence could also be correlated with cancer progression in vivo.
to MATERIALS .AND METHODS
Cell culture. MDA-MB-231 and MCF-7 cells were grown and maintained in Dulbecco's modified Eagle's medium (Life Technologies Ltd, Paisley, UK) supplemented with 4 mM L-glutamine and 10 % foetal bovine serum.
~s Cells were seeded into 100 mm Falcon tissue culture dishes (Becton Dickinson Ltd, Plymouth, UK) and grown in an incubator at 37 oC, 100 humidity and 5 % C02.
Electrophysfology. Patch pipettes (of normal resistances between 5-15 2o MS2) were filled with a solution containing (in mM) NaCl 5, KCl 145, MgCl2 2, CaCl2 l, HEPES 10 and EGTA 11, adjusted to pH 7.4 with 1 M KOH. Whole-cell membrane currents were recorded from cells that appeared 'isolated' in culture using an Axopatch 200B (Axon Instruments) amplifier. Analogue signals were filtered at 5 KHz using a low-pass 2s Bessel filter. Signals were sampled at 5 KHz and digitised using a Digidata (1200) interface. Data acquisition and analysis of whole-cell currents were performed using pClamp (Axon Instruments) software.
Holding potentials of -90 mV or -100 mV were used to tudy K+ and Na+ currents, respectively, unless stated otherwise. Resting potentials were measured immediately following attainment of the 'whole-cell' recording configuration. Experiments on both MDA-MB-231 and MCF-7 cells were performed on three separate dishes which had been plated for s between 1-3 days.
Two basic command voltage protocols were used to study the electrophysiological and pharmacological properties of the Na+ and K+
currents, as follows:
io 1. Current-voltage (I-V) protocol. This protocol was used to study the voltage-dependence of Na+ and K+ channel activation. Cells were pulsed to depolarising test potentials between -70 and +60 mV, in 5 mV
steps. The test pulse duration was 40 ms (Na+ currents) or 200 ms (K+
currents); the interpulse period was 20 s.
is 2. Repeat single-pulse protocol. This was used to monitor the effects of drugs on current amplitude. Test pulses were to -10 mV (Na+
currents) or +60 mV (K+ currents). The test pulse duration was 40 ms (Na+ currents) or 200 ms (K+ currents); the interpulse duration was 20 s and there were 5 repeat pulses.
Pharmacology. Tetrodotoxin (TTX), purchased from Alomone Labs Ltd (Jerusalem, Israel), was made as a stock solution (x1000) in the external bath solution, frozen at -20 oC, defrosted and diluted as required. Briefly, TTX was back-loaded into a glass capillary (with a tip size of -5 Vim). The 2s glass capillary was then connected to a pneumatic picopump (PV 800, WP
Instruments), mounted on a microdrive (Lang-Electronik, Huttenberg, Germany) and manoeuvred to within -10 ~m of the cell under investigation.

~7 The effect of TTX on the inward current (I) has been presented as the percentage block of current (B) in comparison to the control values, calculated as follows:
s B ( % ) _ [(Iafter -Ibefore) / IbeforeJ x 100 VGSCa degenerate primer screens. Total cellular RNA was isolated from two batches of each of the cell lines by the acid guanidium to thiocyanate-phenol-chloroform method (20) or as described below.
Briefly, cells were homogenized in a solution ("A"), using an IKA
homogenises, such that 1 ml of solution was used per 0.1 g of tissue.
Solution A contained 4 M guanidinium thiocyanate, 25 mM Nay citrate (pH 7.0), 0.5 % sarcosyl and 0.72 % (v /v) ~i-mercaptoethanol. The ~s following were then added and shaken vigorously for 10 seconds: 2 M
Na+ acetate, pH 4.0 (10 % volume of solution A), phenol (equal volume of solution A) and chloroform (20 % volume of solution A). Centrifugation was performed at 10,000 x g for 20 rains at 4°C. The supernatant was taken and precipitated with isopropanol. Then, the samples were 2o centrifuged as before and the pellet was resuspended in about 30 % of the initial volume of solution A. A second isopropanol precipitate was performed, the pellet was washed with 75 % ethanol, and resuspended in sterile distilled water.
2s Screens were then performed on each of the four extracts, as described previously (2I and GB 0021617.6, supra), using VGSCa degenerate PCR
primers, YJl and YJ2C (Table 1A).

Twenty five clones with "inserts" were selected by gel electrophoresis for each of the RNA extracts. A subset of the twenty five clones with inserts, derived from each cell line, were then sequenced using the Amersham Thermo Sequenase fluorescent cycle sequencing kit and the Vistra DNA
s 725 automated sequences. Sequences were identified by searching the GenBank DNA database using BLAST 2Ø8 (22). Oligonucleotide primers specific for scn5a and scn9a VGSCas, identified by the sequencing, were subsequently designed (Table 1A). These worked in conjunction with the Universal vector primers and permitted rapid PCR
screening of all other clones without the need for sequencing. PCRs using these primers were initially tested on sequenced clones to confirm that they yielded only specific products. Rapid screening PCR reactions were then performed as in (21) and GB 0021617.6, supra. Products were analysed by gel electrophoresis on 0.8 % agarose gels. Minipreps that did is not test positive for these VGSCa types were sequenced to determine identity.
Table 1. PCR primers used in (A) degenerate VGSCa primer screening, (B) specific PCRs and (C) SQT-PCRs (numbering according to GenBank).
20 Primer annealing positions are indicated in parentheses.
A. Degenerate VGSCa Primer Screening YJ1: - 5' GCGAAGCTT(C/T)TGG(C/T)TIATITT(C/T)I(A/C/G/T)IAT(A/T/C)AT
GGG 3' YJ2C:- 5' 3o ATAGGATCCAICCI(A/C/G/T)I(A/G)AAIGC(A/C/G/T)AC(C/T)TG 3' (40 oC) ScnSa-P1: - 5' TACAATTCTCCGGTCAAGTT 3' (4312-4331; 56 oC) Scn9a-P1: - 5' ATGTTAGTCAAAATGTGCGA 3' (4139-4158; 54 oC) s B. Specific PCR Tests ScnSa-P2: - 5' CATCCTCACCAACTGCGTGT 3' (570-589) ScnSa-P3: - 5' CACTGAGGTAAAGGTCCAGG 3' (1059-1078; 58 ro oC) ScnBa-P1: - 5' AGACCATCCGCACCATCCTG 3' (3855-3874) Scn8a-P2: - 5' TGTCAAAGTTGATCTTCACG 3' (4351-4370; 60 oC) Scn9a-P2: - 5' TATGACCATGAATAACCCGC 3' (474-493) Is Scn9a-P3: - 5' TCAGGTTTCCCATGAACAGC 3' (843-862; 59 oC) hCytbSR-P1: - 5' TATACACCCATCTCCAGCGA 3' (299-318) 2o hCytbSR-P2: - 5' CATCTCCTCATTCACGAAGC 3' (771-790;
60 oC) C. SQT-PCRs 2s ScnSa-P4: - 5' CTGCTGGTCTTCTTGCTTGT 3 ' (2896-2915) ScnSa-P5: - 5' GCTGTTCTCCTCATCCTCTT 3' (3329-3348; 60 o C) ScnBa-P3: - 5' AACCCTATTCCGAGTCATCC 3' (3827-3846) ScnBa-P4: - 5' TGCACTTTCCTCTGTGGCTA 3' (4325-4344; 60 0 3o C) Scn9a-P4: - 5' AAGGAAGACAAAGGGAAAGA 3' (5941-5960) Scn9a-P5: - 5' TCCTGTGAAAAGATGACAAG 3' (6289-6308; 56 0 C) YGSCa-specific PCR tests. These were performed, as in (21) .and GB
0021617.6 in order to ensure that the VGSCas found in the degenerate primer screens were truly expressed in the respective cell lines (and not produced from contaminating genomic DNA).

Briefly, DNA was removed from the extracts by digestion with DNase I
and 5 ~.g of the total RNA was used as the template for single-stranded cDNA (sscDNA) synthesis (Superscript II, GIBCO BRL). sscDNA
s synthesis was primed with a random hexamer mix (R6) in a final volume of 20 ~1. VGSCcc cDNA was then amplified from the R6-sscDNA pool by PCR (Taq DNA polymerase, Amersham Pharmacia) using degenerate PCR primers (YJl and YJ2C) used previously to amplify both Navl and Nav2 VGSCas from adult rat retinal pigment epithelial cells (Dawes, H., to et al (1995) Vis. Neurosci. 12, 1001-1005), and novel VGSCa,s from a protochordate ascidian (Okamura, Y., et al (1994) Neuron 13, 937-948).
PCR reactions were performed on 4 ~.l of the R6-sscDNA template, using 200 pM of each dNTP, 1 unit of Taq, lx Taq buffer and 1 pM of each primer, in a final volume of 20 ~.1. Amplification was via: (i) initial is denaturation at 94 oC for 5 min; (ii) addition of 1 U enzyme; (iii) 33-35 cycles of denaturation at 94 oC for 1 min, annealing at 40 oC for 1 min, and elongation at 72 oC for 1 min; and (iv) elongation at 72 oC for 10 min. For this and aII PCRs performed, reactions with no sscDNA added were also carried out to control for cross-contamination from other DNA
2o sources.
PCR products were analysed by electrophoresis and gel purified prior to Iigation into the pGEM-T vector (pGEM-T Easy Vector System, Promega). These were then used to transform E. coli (pMosBlue, 2s Amersham). Plasmid DNA was recovered from bacterial cultures using a modified version of the Vistra Labstation 625 miniprep procedure (Vistra DNA Systems, Amersham).

Reactions designed to amplify specific VGSCas were performed on both strongly and weakly metastatic cell line extracts, irrespective of whether these subunits had previously been found in degenerate screens. The primer sequences and reaction annealing temperatures used are shown in s Table IB. Evident products were cloned and sequenced, and a consensus sequence for each VGSCa in each cell line then produced (using at least three clones).
Semi-quantitative PCR (SQT-PCR). SQT-PCRs based on kinetic to observation of reactions were performed similarly to (21) and GB
0021617.6.
DNased RNA extracts were used to produce sets of R6-sscDNAs for each extract. 2.4 p.1 of these R6-sscDNAs was used as the template for is VGSCa-specific PCRs (performed as above), in a final volume of 60 ~1.
To allow direct comparison of results obtained from strongly and weakly metastatic cell lines, all comparable R6-sscDNA and PCR reactions were performed simultaneously. 'Blanks', with no template added, were used as controls. PCRs were performed using different 20-mer primers for each of 2o the three VGSCas which did not amplify multiple VGSCa products derived from different splice variants (unlike the specific PCRs above).
The primers and annealing temperatures of the PCRs used are shown in Table IC. scnSa and scn9a VGSCa products did not span conserved intron sites so control PCR reactions were performed for these SQT-PCRs 2s in which the sscDNA template was replaced by an aliquot from a reverse transcription reaction which had no reverse transcriptase added. All products were cloned and sequenced, as above, to ensure that only VGSCa-specific products were amplified.

A kinetic observation approach (45; Hoof et al (1991) Anal. Biochem.
I96, 161-169; Wiesner et al (1992) Biochem. Biophys. Res. Comm. I83, 553-559) was adopted such that an aliquot of 5 ~,l from the 60 ~,l reaction was taken at the end of each amplification cycle, for eleven cycles, while s reactions were held at 72 oC. The amplification cycle at which aliquots were first taken differed depending on the VGSGa studied. These aliquots were then electrophoresed (0.8 % agarose gels) with DNA markers of known concentration. Gels were post-stained for 15 minutes (TBE buffer containing 0.8 ~.g/ml ethidium bromide), and digitally imaged (GDS 7500 to Advanced Gel Documentation System, Ultra-Violet Products). Total product mass (nanograms) in each aliquot was determined by image analysis (1D Image Analysis Software, Kodak Digital Science). Two characteristic stages in each PCR reaction were quantifed:
(1) Threshold PCR cycle number (CND at which a given PCR
is product could just be detected by the image analysis software (default settings).
(2) PCR cycle number at which the exponential phase of the reaction finished (CNe).
Accumulation of reaction product with increasing PCR cycle number 2o follows a sigmoid curve (Kohler, T. (1995).Quantitation of mRNA by Palymerase Chain Reaction, pp 3-14, eds. Kohler, T., Lassner, D., Rost, A.-K., Thamm, B., Pustowoit, B, and Remke, H. (Springer, Heidelberg)). However, the two extremes of this curve were unknown or undetermined for the SQT-PCR data (i.e. the initial mass of cDNA at zero 2s cycles was unknown, and the final product mass at the end of the PCR
undetermined). Thus, a sigmoid curve could not be fitted to the data.
Instead a third-order polynomial equation, which also has only one possible point of inflexion (here corresponding to the end of the exponential phase of the. PCR), was used to approximate a sigmoid curve.

Curve-fitting was performed using STATISTICA (SoftStat Inc.), and the second derivative then calculated, to give CNe. This procedure could be performed successfully, with the calculated values of CNe falling within the data points obtained experimentally (Fig. l). Data are presented as s means and standard errors for each cell line (three repeats on two extracts for each VGSCa.). The values of CNt and CNe were used directly to compare the levels .of expression of each VGSCa in the strongly and weakly metastatic cell lines.
to Mean CNt values were calculated for each of the VGSCas present in MDA-MB-231 and MCF-7 cell extracts using the results of SQT-PCRs on both cell batches (except for scn9a, amplified from only one of the two MCF-7 cell line batches, and scn5a, which was apparently expressed too lowly in one MCF-7 batch far CNe to be calculated). Assuming that the is PCR reactions performed on strongly and weakly metastatic cell RNA
extracts had similar efficiencies, differences in the calculated CNt and CNe values would reflect real differences in expression levels.
NADH-cytochrome b5 reductase (CytbSR), which is expressed at very 2o similar levels in normal, cancerous and strongly metastatic cells derived from numerous tissue types (20; Fitzsimmons, S.A., et al (1996) J. Natl.
Cancer Inst. 88, 259-269; Marin, A., et al (1997) Br. J. Cancer 76, 923-929), was present in both rat and human degenerate primer screens as a major constituent of the non-specific products found (the "non-VGSCa"
25 clones). Consequently, this was used as a control amplicon in SQT-PCRs, ie to control for the effects of variations in quality and quantity of the initial RNA, efficiency of the reverse-transcription and amplification between samples (primers are shown in Table 1B). CytbgR 20-mer primers amplified nucleotides 38S-809 and 299-790 of rat and human homologues, respectively (annealing temperature, 60 oC for both).
PCR tests on breast biopsy tissue. 0. I - 0. S g pieces of frozen tissue s were chopped into small pieces using a sterile scalpel and forceps and placed in a cold, glass homogenizes. Total cellular RNA was then isolated as described above. RNA quality was preliminarily assessed by gel electrophoresis and quantity determined by spectrophotometric analysis.
to RNA extracts were then used as the template for sscDNA synthesis, performed as above. The possible expression of scn5a, scn8a and scn9a RNAs in the biopsy samples was tested by PCR, using the same primers as for the specific PCRs (Table 1B). hCytbSR specific PCR tests were also carried out to further control for the quality of the extracted RNA;
is samples which did not yield evident hCytbSR products were rejected.
PCRs were performed, using 2.5 ~.l of the synthesised sscDNA, 0.2 millimolar dNTPs, 1 micomolar of each specific primer and 1 unit of Taq, under the following conditions: 94 °C for S min; 1U enzyme added; 94 °C
for 1 min; 59 - 62 °C for 1 min (depending on the primer pair); 72 °C 1 2o min; final incubation at 72 °C for 10 min with the main section repeated 30-60 times (depending on the primer pair). PCR reactions with no template added were also performed to control for cross-contamination from other DNA sources. S ~.1 aliquots of the final reaction were analysed by gel electrophoresis on 0.8 % agarose gels.
PCR tests were carried out on each of at least two cDNA templates (except for sample 1, from which only S ~.g of RNA was obtained), manufactured independently from the same RNA extract, thus controlling for possible variability in cDNA manufacture and PCR efficiency.

Data analysis. All quantitative data were determined to be normally distributed and are presented in the text as means ~ standard errors.
Statistical significance was determined with Student's t test or x2 test, as s appropriate.
GenBank sequence nucleotide numbers. Nucleotide numbering was according to accession numbers M77235, AB027567, X82835, Y09501 for scn5a, scn8a, scn9a and hCytbSR, respectively.
RESULTS
Electrophysiological studies. The average resting potential of MDA-MB-231 cells was -18.9 ~ 2.1 mV (n=27; range -12 to -61 mV) which was significantly more depolarised than the value of -38.9 ~ 2.5 mV
is (n=26; range -8 to -51 mV) for the MCF-7 cells (p < 0.001). The membrane capacitance of the MDA-MB-231 cells was 28.5 ~ 2.7 pF
(n=35; range 14.7 to 76.6 pF) which was significantly smaller than the value of 36.9 ~ 2.8 pF (n=38; range 13.5 to 90.0 pF) for the MCF-7 cells (p < 0.05).
29 % of the MDA-MB-231 cells tested (n=16/56) expressed an inward current of up to 600 pA in amplitude (Figure 1A), which corresponded to a current density of 5.6 ~ 0.5 pAlpF (n=16). The inward currents activated at -41.3 ~ 2.4 mV (n=4), peaked at -6.3 2.4 mV (n=4; Figure 2s 1C) and were abolished in Na+-free medium (not shown; n=2), consistent with them being VGS currents. In contrast, none of the MCF-7 cells tested (n=72) showed an inward current (Figure 1B).

The VGS current was suppressed partially by micromolar TTX (Figure 2A). The effect of the toxin was concentration dependent in the range 100 nM - 6 pM (Figure 2B). However, even at the highest concentration used (6 p.M), only 64.7 ~ 6.1 % of the current was blocked by TTX (n=5).
s There was a small (9 ~ 3 % ) reduction in peak current with 100 nM TTX, which was significant (p < 0.05), indicating that a minor, TTX-sensitive (TTX-S) component was also present (Figure 2B).
Voltage-gated outward currents were also recorded. 100 % of the .MCF-7 cells tested (n=72) expressed large outward currents of up to 7 nA in amplitude (Figure 1B), which corresponded to a current density of 27.4 ~
4.9 pA/pF (n=33). These outward currents activated at -9.2 ~ 1.9 mV
(n=12) and showed a peak amplitude of 1081.1 ~ 264.7 pA at +90 mV
(n=12). The current was reduced to 34.3 + 5.4 pA (n=15; p < 0.01), i.e.
is by 97 % , by substituting Cs + fox K+ in the internal pipette solution. In comparison, MDA-MB-231 cells showed much smaller outward currents of up to 150 pA (n=35; Figure 1B), which corresponded to a current density of only 2.6 ~ 0.4 pA/pF (n=13; p < 0.01 cf. comparable currents recorded in the MCF-7 cells).
YGSCa mRNA expression in the cell lines. The results of the degenerate-primer screens for the different cell line RNA extracts are shown in Table 2. Two VGSCas were identified in the screens on the strongly metastatic cell line: products of SCNSA and SCN9A VGSCa 2s genes. In contrast, scn8a was the only VGSCa found in the degenerate screens of the weakly metastatic MCF-7 cells. It has previously been shown that for Navl VGSCas, the proportion of clones in degenerate primer screens representing each VGSCa type reflects the actual proportion of that subunit within the cellular VGSCa mRNA pool (21).
Thus, in the strongly metastatic cells, screen results indicated that scn5a (56.0 ~ 4.0 %) was expressed at a much greater level than scn9a (I2.0 +
4.0 % ) and scn8a (0 % ) (Table 2).
s Table 2. Summary of the VGCSa degenerate primer screen results.
Results are shown as percentage of clones tested (n=25 in each case).
Each screen is the result of two extracts from each cell line. Errors indicate standard errors.
to YGSCa MDA-MB-231 MCF-7 ScnSa 56.0 + 4.0 0 ScnBa 0 2.0 + 2.0 Scn9a 12.0 + 4.0 0 Non - VGSCa, 32.0 ~ 0 98.0 ~ 2.0 Primer-specific PCRs yielded products for scn5a, scn8a and scn9a (as well as for hCytbSR) in both cell lines, indicating that all of these mRNAs 1s were expressed in both MDA-MB-231 and MCF-7 cells. However, scn5a and scn9a required markedly Iess amplification (CNt) to yield detectable products and reach CNe in SQT-PCRs on MDA-MB-231 vs. MCF-7 cell extracts, indicating an overall greater level of expression in the strongly metastatic cells (Figure 3A and 3C). Importantly, the most striking, 2o consistent difference was seen for SCNSA: CNt = 24.75 + 0.48 vs.
37.50 + 1.56; CNe = 28.36 + 0.46 vs. 38.54 + 0.14, fox MDA-MB-23I s vs. MCF-7 cells, respectively (Figure 3A). Assuming an 80 % PCR
efficiency (21), this would indicate ~' 1800-fold difference in expression levels between the two cell lines.
s Scn9a was more readily amplified in the strongly metastatic (CNt = 30.75 + 0.63; CNe = 34.44 ~ 0.65) than the weakly metastatic cells (CNt =
42.5 + 4.5; CNe = 46.0 ~ 3.2), but this TTX-S VGSCa was not as prominent as scn5a in degenerate screens, indicating a lower level of expression. In contrast, hCytbSR 'control' and scn8a SQT-PCRs showed to very similar levels of expression in both MDA-MB-231 and MCF-7 cells (Figure 3B and 3D): CNt = 20.25 + 0.25 vs. 22.0 ~ 0.56, CNe =
23.96 ~ 1.00 vs. 25.16 + 0.34, for hCytbSR; CNt = 33.25 + 0.25 vs. 32.75 + 0.63, CNe = 36.85 + 0.32 vs. 35.61 + 0.49, for scn8a.
Importantly, hCytbSR was the major constituent of the 'non-Is VGSCa'clones found in the degenerate screens, representing almost all of the non-VGSCa clones (equivalent to 28.0 ~ 0 % of all clones) in the MDA-MB-231 cells and 54.0 + 6.0 % of all the clones in the MCF-7 cell line screen. The increased incidence of this non-VGSCa clone in the degenerate screens of the MCF-7 cells is consistent with a lower VGSCa 2o target to noise ratio in these cells compared to their strongly metastatic counterpart, also evident from the SQT-PCR data.
The MDA-MB-231/MCF-7 VGSCa sequences obtained have been submitted to GenBank.
VGSCa mRNA expression in breast biopsy tissue. RNA was extracted successfully, with positive hCytbSR tests obtained, from I2 samples.
Generally, PCR results of the VGSCa and hCytbSR control tests were readily repeatable across different synthesised cDNA batches. The results obtained are summarised in Table 3. All three VGSCa genes found to be expressed in the cell lines were detected in the biopsy samples, confirming the conservation in vivo of the VGSCa, expression profile of the in vitro models. Several SCNBA and SCN9A products (corresponding to different s splice forms of these genes; (21)) were amplified from all samples (Figure 4 D and E), as was the hCytbSR control (Figure 4F), except in sample 6.
It is likely that the RNA extracted from this sample was significantly more degraded than the other samples, as evidenced by the greater number of PCR cycles required to amplify the hCytbSR control product (40 not 30 to cycles). There was, however, no evident correlation between scn8a or scn9a expression and lymph node metastasis (LNM). In contrast, expression of scn5a was strictly sample-dependent (Figure 4A and B). All evident products of these tests were cloned and sequenced, and it was verified that these products were truly derived from SCNSA. ScnSa ~s VGSCa, sequences obtained from these samples have been submitted to GenBank.
Accession numbers of submitted sequences are as follows:
Accession: AJ310882 2o Description: Homo sapiens partial mRNA for voltage-gated sodium channel Navl.7(SCN9A gene) cell line MDA-MB-231 Accession: AJ310883 2s Description: Homo sapiens partial mRNA for Navl.7 voltage-gated-sodium channel(SCN9A gene) cell line MCF-7 Accession: AJ310884 3o Description: Homo Sapiens mRNA for Navl.6 voltage-gated-sodium (SCNBA
gene)Navl.6, D3 neonatal splice variant, cell lines MDA-MB-231 Accession: AJ310885 Description: Homo sapiens partial mRNA for voltage-gated sodium channel Navl.6(SCNBA gene), D3 neonatal splice variant, cell line MCF-7 s Accession: AJ310886 Description: Homo Sapiens partial mRNA for voltage gated sodium channel Navl.S(SCNSA gene), D1 neonatal splice variant, cell line MDA-MB-231 to Accession: AJ310887 Description: Homo sapiens partial mRNA for voltage-gated sodium channel Navl.S(SCNSA gene) Dl neonatal splice variant, cell line MCF-7 is Accession: AJ310888 Description: Homo sapiens partial mRNA for voltage gated sodium channel Navl.S(SCNSA gene), D1 neonatal splice variant, biopsy sample 2 2o Accession: AJ310889 Description: Homo sapiens partial mRNA for voltage-gated sodium channel Navl.S(SCNSA gene), D1 neonatal splice variant, biopsy sample 3 2s Accession: AJ310890 Description: Homo Sapiens partial mRNA for voltage-gated sodium channel Navl.S(SCNSA gene) Dl adult splice variant, biopsy sample 1 3o Accession: AJ3I089I
Description: Homo sapiens partial mRNA for voltage gated sodium channel Navl.S(SCNSA gene), Dl adult splice variant, biopsy sample 7 Ss Accession#: AJ310892 Description: Homo sapiens partial mRNA for voltage-gated sodium channel Navl.S(SCNSA gene), biopsy sample 6 4o Accession: AJ310893 Description: Homo Sapiens partial mRNA for voltage gated sodium channel Navl.S(SCNSA gene), D1:S3 exon-skipped splice variant, biopsy sample Accession: AJ310894 s Description: Homo sapiens partial mRNA for voltage-gated sodium channel Navl.S(SCNSA gene), D1 neonatal splice variant, biopsy sample 4 Accession: AJ310895 zo Description: Homo sapiens partial mRNA for NavI.S (scn5a/hl) voltage-gated sodiumchannel (SCNSA gene), D1 neonatal splice variant, biopsy sample S
~s Accession#: AJ310896 Description: Homo Sapiens partial mRNA for voltage-gated sodium Navl.S
(SCNSA gene)(SCNSA gene), cell line MDA-MB-231 2o Accession: AJ310897 Description: Homo sapiens partial mRNA for voltage-gated sodium Navl .7 (SCN9A gene)(SCN9A gene), cell line MDA-MB-231 2s Accession: AJ310898 Description: Homo sapiens partial mRNA for voltage-gated sodium channel Navl.6(SCNBA gene), cell line MCF-7 so Accession: AJ310899 Description: Homo Sapiens partial mRNA for NADH-cytochrome bS
reductase (BSR gene)cell line MDA-MB-231 3s Accession: AJ310900 Description: Homo sapiens partial mRNA for NADH-cytochrome bS
reductase (BSR gene)cell line MCF-7 Sequences have also been submitted for the following:
scn5a MDA-MB-231 SQT-PCR sequence scn5a MCF-7 SQT-PCR sequence scn9a MDA-MB-231 SQT-PCR sequence (3UTR) scn9a MCF-7 SQT-PCR sequence (3UTR) Some specific points regarding the SEQUENCE data:
s SCNSA - Three nucleotide differences from the published sequence (GenBank M77235) in the sequence obtained outside the D1 neonatal exon: 689/690 (CT to GC) differences would substitute an alanine for a glycineresidue at amino acid position 180 (all numbering according to to M77235). All other voltage-gated sodium channel alpha subunit genes have a glycine at this residue, and thus it is most probable that the published sequence (M77235) contains sequence errors at this location.
992 (T to C) difference would not change the amino acid sequence and may represent a natural, silent-polymorphism in the SCNSA gene.
Is SCNBA - No nucleotide differences from our previously published sequences [SCNBA in prostate cancer cell lines] (GenBank AJ276141 and AJ276142).
20 SCN9A - No nucleotide differences from the published sequence (GenBank X82835).
The SCNSA D1 neonatal exon - this could be clinically important. This is the first report of the existence of an apparent alternative splice form of 25 scn5a at this location. The SCNSA gene structure has been investigated previously (Wang et al., 1996), using scn5a cDNA sequences to probe a human genomic library but alternative exons for D1S3 were not found, presumably because the hybridizing cDNAs were of the known adult rather than the neonatal form. The scn5a neonatal form differs from the previously published adult form (Gellens et al. , 1992; GenBank M77235) at 31 of the 92'nucleotides in this conserved exon. These 31 nucleotide differences in the neonatal SCNSA form result in 7 amino acid substitutions, many more than observed for the other VGSC alpha subunit s genes studied thus far.
To date, alternative splicing of neonatal and adult exons has been found in D1S3 in four other VGSC alpha genes: SCN2A, SCN3A, SCNBA and SCN9A. In each of these instances the alternative . exons have 19-21 to nucleotide differences, which result in 1-2 amino acid substitutions. One amino acid substitution at residue seven of this exon is consistent across all of these genes: the substitution of an aspartate residue in the adult form to a neutral amino acid in the neonatal form. Alternative splicing in scn5a was not completely consistent with D1S3 splicing previously described for is other VGSC alphas in two main ways:
(1) In the scn5a neonatal form, the aspartate residue in the adult form was not substituted for a non-charged amino acid, but a positively charged lysine residue.
(2) The 31 nucleotide differences in the neonatal scn5a result in 7 amino 2o acid substitutions, many more than the 1-2 amino acid substitutions observed for the other VGSC alpha genes with alternative splicing at D1S3, previously studied.
Table 3. Summary of results of specific RT-PCR tests on breast cancer 2s biopsy samples. (+) indicates that a specific product was obtained; (-) indicates that no specific product was amplified; NT that the test was not performed; ND that the grade of the tumour was not determined. PCR
tests were performed for up to 55, 50 and 50 cycles for scn5a, scn8a and scn9a, respectively. hCytbSR tests were performed for 30 cycles except for sample 6, for which 40 cycles were used (denoted by *). Clinically assessment lymph node metastasis (LNM) and tumour grade are also shown for each case. For LNM (+), the values in parentheses indicate the number of lymph nodes which were determined as positive / number of s nodes examined.
Sample Clinical hCytbSR scn8a scn9a ScnSa LNM
Grade 1 2 + NT + + + (4/4) 2 3 + + + + + (3/7) 3 2 + + + + + (3/7) 4 2 + + + + + (8112) S ND + + + + + (8/13) 6 2 +(*) - - + + (9/14) 7 1 + + + + - (0/22) 8 2 + + + + - (0/13) 9 2 + + + - - (0/15) I 3 + + + - - (0/
0 10) 11 1 + + + - - (0/
15) 12 3 + + + - - (0/9) A 'double-blind' test associating scn5a expression with LNM revealed that these two characteristics were directly correlated in 10 out of the 12 (83 cases examined, giving combinations of scn5a+/LNM+ (n=6) and scn5a-/LNM- (n=4) (x2 = 6.0; df=1; 0.02 > p > 0.01; Table 3). One of the two exceptions where the sample was scn5a+ but apparently LNM-15 (sample 7) was interesting in that the patient subsequently relapsed, developing distant metastases within three years of the preliminary diagnosis. Whether relapse also occurred for the patient who provided the other exceptional case (sample ~) could not be determined.
s DISCUSSION
The present study shows (i) that strongly, but not weakly metastatic breast cancer cells displayed VGS currents, almost entirely composed of a TTX-resistant (TTX-R) component; (ii) that a particular TTX-R VGSCa gene, SCNSA, was predominantly expressed in strongly metastatic cells, but 1o expressed at only very low levels in weakly metastatic cells; and (iii) that scn5a expression in biopsy samples correlated strongly with clinically assessed lymph node metastasis. Furthermore, the high-level VGSC
expression was accompanied inversely by much reduced outward currents in the cell lines, and a relatively depolarised resting potential. Taken is together, these characteristics would render metastatic cell membranes potentially 'excitable', consistent with their hyperactive behaviour.
ScnSa expression is associated with breast cancer metastasis. The electxophysiological and RT-PCR results demonstrated consistently that 2o SCNSA gene products (also termed hl or SkM2) were predominantly expressed in the strongly metastatic cell line (Figure 5) and were associated with breast cancer metastasis in vivo. We have shown previously for human and/or rat prostate cancer cells that VGSC activity contributes to cellular behaviours integral to metastasis, including cellular 2s process extension (7), lateral migration (S), transverse invasion (5,6,9) and secretory membrane activity (10). Subsequently, scn9a was identified as the 'culprit' VGSCa (21). In the present study, the correlation of scn5a expression with increased cellular metastatic potential ifa vitro, and lymph node metastasis in vivo, would strongly indicate a significant role for scn5a activity in the metastatic behaviour of breast cancer cells.
Although the present study is the first to associate scn5a with cellular s metastatic potential, others have previously reported expression of this VGSCa in cancer cell lines. ScnSa mRNA and functional protein expression have been shown to occur in B104 neuroblastoma cells (25) and RT4 peripheral neurotumour cancer cell lines (26,27). At present, it is not clear why strongly metastatic cells from carcinomas derived from 1 o different tissues should specifically upregulate the expression of different VGSCas. Also, it is not known if, amongst the various VGSCas, only scn5a would be capable of potentiating metastasis in breast carcinoma. If so, then it may be that this ability results from characteristics peculiar only to this VGSCa. Such possible, characteristic features of scn5a include the is following: (i) Possession of C-terminal PDZ domains (28,29), potentially enabling particular interaction with the cytoskeleton; (ii) extremely low level of protein glycosylation (5 % of the total protein mass, compared to up to 40% protein mass in other VGSCas; (30)); (iii) highly promiscuous ion selectivity in given conditions, allowing Ca2+ entry (31); (iv) very 2o slow activation and inactivation kinetics (28,32); and (v) regulation of expression by steroid hormones (33).
Another notable characteristic of scn5a is that its expression appears to be under very tight spatio-temporal control and highly dynamic regulation.
SCNSA gene products are classically expressed at very high levels in 2s cardiac and neonatal/denervated skeletal muscle (29,34). However, ScnSa mRNA has also been detected in non-excitable, cultured spinal cord astrocytes (35) but not in a variety of cell types which express almost all other VGSCas, like dorsal root ganglion neurones (26,36,37).
Furthermore, in skeletal muscle particularly, significant changes in expression levels can occur over a period of only days after birth (34) and in response to denervation (38).
Conservation of breast cancer YGSCa expression in biopsy tissue. The s profile of VGSCa expression in weakly and strongly metastatic breast cancer cells that we have obtained from the two Bell lines (Figure 5) is consistent with the results of the PCRs performed on the biopsy tissues.
All three VGSCas found in the cell lines were found to be expressed in the tissue samples and the expression of the predominant scn5a type was 1o correlated strongly with the surgically characterised metastases.
Although relative expression levels of scn5a, scn8a and scn9a cannot dixectly be determined from the PCR tests, the apparent ease of amplification of the different VGSCas from the biopsy tissues is consistent Is with the biopsy samples consisting almost entirely of a mass of essentially non-metastatic primary tumour cells with only a very small number of strongly metastatic cancer cells present in malignant tumours (e.g. 39,40).
Thus, scn8a and scn9a (which are expressed at greater levels than ScnSa in weakly metastatic cells) could be detected in biopsy tissue using a lower 2o number of PCR cycles, compared with scn5a, even from samples displaying evident lymph node metastasis.
The PCRs performed on the biopsy tissues did not yield reliable quantitative information concerning expression levels of the various VGSCas, mainly due to the large variability in the quality of extracted 2s RNA from one sample to another, as monitored by the control RNA.
ScnSa expression was apparently so low in weakly metastatic cells that it could not be detected in non-malignant biopsy tissues. However, the expression, being greatly upregulated in the strongly metastatic cells within the biopsies, became readily detectable by PCR.

Multiplicity of YGSCa expression in breast cancer cell lines. The expression of multiple VGSCa genes was determined in both breast cancer cell lines and is consistent with the relative VGSCa expression s profiles illustrated in Figure 5. In brief, the level of scn8a was similar for both cell lines but very low, whilst expression of scn5a and scn9a were significantly greater for the MDA-MB-231 cell line. In particular, scn5a expression accounted for > 80% of the VGSCas in these cells. A D1 neonatal splice form of SCNSA may be of clinical importance, as to discussed above. Multiplicity of VGSCa expression has also been found in rat and human prostate cancer cell lines of differing metastatic potential (21). The pharmacological data (TTX blockage) indicated that the VGS
currents detected in the MDA-MB-231 cells were mainly TTX-R (ICSO
> 1 ~M). This is consistent with the determined mRNA expression profile Is of these cells in which the TTX-R scn5a VGSCa is the predominant channel. The scn9a VGSCa expressed, but at much lower levels (Figure 5), would yield TTX-S currents which could contribute to the TTX
sensitivity observed at lower (100 nM) concentrations. Possible consequences of multiple VGSCa expression have been discussed 2o previously (21). Interestingly, the full-length scn8a products detected in both strongly and weakly metastatic breast cancer cells were the neonatal splice form as determined by the product size (Diss, J.K.J., unpublished observation). This form of scn8a codes for a highly truncated VGSCa protein, and has been found to be preferentially expressed in neonatal and 2s non-excitable adult tissues (41). Neonatal scn8a is thought not to be capable of creating functional VGSCs, instead acting as a "fail-safe"
mechanism, preventing the functional expression of leakily expressed, non-truncated scn8a VGSCas. The detection of neonatal scn8a mRNA in biopsy samples (as determined by the product size; Figure 4D) indicates that this mechanism is also present in vivo.
VGSC expression in breast and prostate cancer: Comparative aspects.
s Many aspects of the findings of this study are similar to those determined using similar techniques in rat and human prostate cancer cell lines of differing metastatic potential (5,6,21): (i) the strongly metastatic cells had relatively depolarised resting potentials (6). (ii) VGS currents were detected in a sub-population of strongly metastatic cells (54 % MAT-LyLu, l0 10% PC-3, 29% MDA-MB-231) and never detected in corresponding weakly metastatic cells (AT-2, LNCaP, MCF-7); (iii) VGSCa mRNA was detected in cells of both strong and weak metastatic potential, but with greater expression in strongly metastatic cells; (iv) multiple VGSCa expression was determined in all cells; (v) all cells expressed scn8a, is mainly in the non-functional, neonatal form (21); and (vi) the predominant VGSCa (scn9a in prostate cancer cells; scn5a in breast cancer cells) was expressed more than 1000-fold more in strongly vs. weakly metastatic cells.
2o That we should find a similar mechanism potentially involved in metastasis of both breast and prostate cancer is not completely surprising in view of their similarities in tumour biology (e.g. hormone-responsiveness and propensity for bone metastasis) but does strongly encourage future work to investigate VGSC activity and metastasis in 2s other cancer types. VGSCa, expression has been determined in developing small cell carcinoma of ~ the lung (42) and gliomas (43,44). Thus, functional VGSC expression may be part of a general mechanism for cancer progression and metastasis.

On the other hand, it is unclear why a specific, but different VGSCa, should be associated with metastasis in breast and prostate cancers. Whilst not intending to be bound by theory, it is possible that all VGSCas may have the capability of potentiating the metastatic cascade, or only specific s types (including scn5a and scn9a). AlI VGSCas that are capable of potentiating metastasis may affect the same basic cellular processes) within the cascade, for example cellular process extension, lateral migration, secretion or transverse invasion. The specific association of a pairticular VGSCa with metastasis in a given cancer type may result from to tissue- (or cancer-) specif'lc transcriptional regulation/control mechanisms, for example androgens in prostate cancer or oestrogen in breast cancer.
Alternatively, this specific association may result from different VGSCcx(s) affecting different cellular processes which may be more or less important for successful metastasis from different primary tumour Is sites.
Clinical implications. Prior to the present invention, only indirect indicators as to the likelihood of metastatic potential were available, since, although it is possible to detect micrometastases in a proportion of patients 2o with breast cancer, many patients who do not have micrometastases at presentation eventually develop overt metastatic disease during follow-up (45). Consequently, clinicians, therefore, require a more accurate method for predicting the likelihood of development of metastatic disease, and the presence of VGSCs could act as an independent prognostic parameter in a 2s multivariant approach to this problem. Of perhaps greater significance in the future is the potential implications of inhibiting VGSC activity. The scn5a VGSCoc is already the specific target of numerous anti-arrhythmic and anti-convulsant drugs, since dysfunction of scn5a in cardiac tissue is intricately linked to several forms of heart disease and arrhythmia (46).

Interestingly, the breast cancer drug tamoxifen has been found also to protect the heart (47) although it is not known if this involves VGSC
modulation (48). The present work, which identifies scn5a as the potential 'culprit' VGSCa in breast cancer metastasis, therefore, indicates that s scn5a-specific drugs may be inhibitors of the metastatic cascade.
Sequence information The question of whether there is any difference in the sequences of the 'wild type' and the 'breast cancer culprit' SCNSA
gene is an important one. In general, there are two major reasons that to suggest that differences in sequence are less important than differences in level of expression: (a) The sequence data that we have obtained so far shows identity (note however that our data represent at the most only some 17 % and often less than 10 % of the whole sequence) and (b) the expression levels are > 1000-fold different between the strongly vs the zs weakly metastatic cells. Taken together, we think that it is the level of expression (and whatever is responsible for it) rather than sequence differences) that is important. Of course, there may be some sequence differences that are important for cancer. To test that would require complete sequencing of the gene which is not a trivial exercise. There are 2o examples of quite subtle nucleotide changes in VGSC genes giving rise to profound changes in function, leading to a pathological condition [see J.
Physiol. (2000) 529:533-539, for a recent examples .
2s REFERENCES
1. Parkin, D.M., Pisani, P. and Ferlay, J. Estimates of the worldwide incidence of 25 major cancers in 1990. Int. J. Cancer, 80: 827-841, 1999.

2. Schwirzke, M., Schiemann, S., Gnirke, A. and Weidle, U. New genes potentially involved in breast cancer metastasis. Anticancer Res., 19:
1801-1814, 1999.
3. Wingo, P.A., Ries, L.A., Rosenberg, H.M., Miller, D.S. and s Edwards, B.K. Cancer incidence and mortality, 1973-1995 - A report card for the US. Cancer, ~2: 1197-1207, 1998.
4. Cuzick, J. , Holland, R. , Barth, V . , Davies, R. , Faupel, M. , Fentiman, I., Frischbier, H.J., LaMarque, J.L., Merson, M., Sacchini, V., Vanel, D. and Veronesi, U. Electropotential measurements as a new lo. diagnostic modality for breast cancer. The Lancet, 352: 359-363, 1998.
S. Grimes, J.A., Fraser, S.P., Stephens, G.J., Downing, J.E.G., Laniado, M.E., Foster, C.S., Abel, P.D. and Djamgoz, M.B.A.
Differential expression of voltage-gated Na+ currents in two prostatic tumour cell lines: contribution to invasiveness in vitro FEBS Letts., 369:
Is 290-294, 1995.
6. Laniado, M., Lalani, E.N., Fraser, S.P., Grimes, J.A., Bhangal, G., Djamgoz, M.B.A. and Abel, P.D. Expression and functional analysis of voltage-activated Na+ channels in human prostate cancer cell lines and their contribution to invasiveness in vitro. Am. J. Pathol., I50: 1213-20 1221, 1997 .
7. Fraser, S.P., Ding, Y., Liu, A., Foster, C.S. and Djamgoz, M.B.A.
Tetrodotoxin suppresses morphological enhancement of the metastatic MAT-LyLu rat prostate cancer cell line. Cell Tissue Res., 295: 505-512, 1999.
2s 8. Fraser, S.P., Salvador, V. and Djamgoz, M.B.A. Voltage-gated Na+
channel activity contributes to rodent prostate cancer migration in vitro. J.
Physiol., 513P: 13IP, 1998.
9. Smith, P., Rhodes, N.p,, Shortland, A.P., Fraser, S.P., Djamgoz, M.B.A., Ke, Y. and Foster, C.S. Sodium channel protein expression enhances the invasiveness of rat and human prostate cancer cells. FEBS
Letts. , 423: 19-24, 1998.

10. Mycielska, M., Fraser, S.P., Szatkowski, M. and Djamgoz, M.B.A.
Endocytic membrane activity in rat prostate cancer cell lines: potentiation s by functional voltage-gated sodium channels. J. Physiol., (In the press).

11. Rodriguez, C., Calle, E.E., Tatham, L.M., Wingo, P.A., Miracle-McMahill, H.L., Thun, M.J. and Heath, C.W. Family history of breast cancer as a predictor for fatal prostate cancer. Epidemiology, 9: 525-529, 1998.
l0 12. Catterall, W.A. From ionic currents to molecular mechanisms: the structure and function of voltage-gated sodium channels. Neuron, 26: 13-25, 2000.
13. Goldin, A. L., Snutch, T., Lubbert, H., Dowsett, A., Marshall, J., Auld, V., Downey, W., Fritz, L.C., Lester, H. A., Dune, R., Catterall, ~s W. A. and Davidson, N. Messenger-RNA coding for only the alpha subunit of the rat-brain Na-channel is sufficient for expression of functional channels in Xenopus-oocytes. Proc. Natl. Acad. Sci. USA, 83:
7503-7507, 1986.
14. Isom, L.L., De Jongh, K.S., Patton, D.E., Reber, B.F.X., Offord,J., 2o Charbonneau, H., Walsh, K., Goldin, A.L. and Catterall, W.A. Primary structure and functional expression of the beta-1-subunit of the rat-brain sodium-channel. Science, 256: 839-842, 1992.
15. Morgan, K., Stevens, E.B., Shah, B., Cox, P.J., Dixon, A.K., Lee, K., Pinnock, R.D., Hughes, J., Richardson, P.J., Mizuguchi, K. and 2s Jackson, A.P. 3: An additional auxiliary subunit of the voltage-sensitive sodium channel that modulates channel gating with distinct kinetics. Proc.
Natl. Acad. Sci. USA., 97: 2308-2313, 2000.
16. Plummer, N.W. and Meisler, M.H. Exon organization, coding sequence, physical mapping, and polymorphic intragenic markers for the human neuronal sodium channel gene SCNBA. Genomics, 57: 323-331, 1998.
17. Jeong, S.Y., Goto, J., Hashida, H., Suzuki, T., Ogata, K., Masuda, N. , Hirai, M. , Isahara, K. , Uchiyama, Y. and Kanazawa, I. Identification s of a novel human voltage-gated sodium channel alpha subunit gene, SCN12A. Biochem. Biophys. Res. Commun., 267: 262-270, 2000.
18. Soule, H.D., Vasguez, J., Long, A., Albert, S. and Brennan, M. A
human cell line from a pleural effusion derived from a breast carcinoma.
J. Natl. Cancer Inst. , Sl : 1409-1416, 1973.
19. Zhang, R.D., Fidler, LJ. and Price, J.E. Relative malignant potential of human breast-carcinoma cell-lines established from pleural effusions and a brain metastasis. Invasion Metastasis, 11: 204-215, 1991.
20. Chomczynski, P. and Sacchi, N. Single-step method of RNA
isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
is Ann. Biochem., 162: 156-159, 1987.
21. Diss, J.K.J., Archer, S.N., Hirano, J., Fraser, S.P. and Djamgoz, M.B.A. Predominant expression of the SCN9A voltage-gated Na+
channel a-subunit gene in strongly metastatic cell lines of both rat and human prostate cancer: Semi-quantitative PCR analyses. Prostate (In 2o press).
22. Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D.J. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. , 25:
3389-3402, 1997.
2s 23. Fitzsimmons, S.A., Workman, P., Grever, M., Paull, K., Camalier, R. and Lewis, A.D. Reductase enzyme expression across the National Cancer Institute Tumor cell line panel: correlation with sensitivity to mitomycin C and E09. J. Natl. Cancer Inst. , 88: 259-269, 1996.

24. Maria, A., Lopez de Cerain, A., Hamilton, E., Lewis, A.D., Martinet-Penuela, J.M., Idoate, M.A. and Bello, J. DT-diaphorase and cytochrome BS reductase in human lung and breast tumours. Br. J.
Cancer, 76: 923-929, 1997.
s 25. Gu, X.Q., Dib-Hajj, S.D., Rizzo, M. and Waxman, S.G. TTX-sensitive and -resistant Na+ currents, and mRNA for the TTX-resistant rH1 channel, are expressed in B104 neuroblastoma cells. J.
Neurophysiol. , 77: 236-246, 1997.
26. Donahue, L.M., Schaller, K. and Sueoka, N. Segregation of Na+
lo channel gene-expression during neuronal glial branching of a rat PNS
derived stem-cell line, RT4-AC. Devel. Biol., 147: 415-424, 1991.
27. Zeng, D.W., Kyle, J.W., Martin, R.L., Ambler, K.S. and Hanck, D.A. Cardiac sodium channels expressed in a peripheral neurotumor-derived cell line, RT4-B8. Am. J. Physiol. , 39: C 1522-C 1531, 1996.
Is 28. Gellens, M.E., George, A.L., Chen, L.Q., Chahine, M., Horn, R., Barchi, R.C. and Kallen, R.G. Primary structure and functional expression of the human cardiac tetrodotoxin-insensitive voltage-dependent sodium-channel. Proc. Natl. Acad. Sci. USA., 89: 554-558, 1992.
29. Rogart, R.B., Cribbs, L.L., Muglia, L.K., Kephart, D.D. and Kaiser, 2o M.W. Molecular-cloning of a putative tetrodotoxin-resistant rat-heart Na+ channel isoform. Proc. Natl. Acad. Sci. USA., 86: 8170-8174, 1989.
30. Cohen, S.A. and Levitt, L.K. Partial characterization of the rHl sodium-channel protein from rat-heart using subtype-specific antibodies.
2s Circulation Res., 73: 735-742, 1993.
31. Santana, L.F., Gomez, A.M. and Lederer, W.J. Ca2+ flux through promiscuous cardiac Na+ channels: Slip-mode conductance. Science, 279: 1027-1033, 1998.

32. White, M.M. , Chen, L. Q. , Kleinfield, R. , Kallen, R. G. and Barchi, R.L. Skm2, a Na+ channel cDNA clone from denervated skeletal-muscle, encodes a tetrodotoxin-insensitive Na + channel. Mol. Pharmacol. , 39:
604-608, 1991.
s 33. Rich, M.M., Kraner, S.D. and Barchi, R.L. Altered gene expression in steroid-treated denervated muscle. Neurobiol. Dis., 6 : 515-522, 1999.
34. Kallen R.G., Sheng Z.H., Yang J., Chen L.Q., Rogart R.B. and Barchi R.L. Primary structure and expression of a sodium-channel characteristic of denervated and immature rat skeletal-muscle. Neuron 4:
l0 233-242, 1990.
35. Black, J.A., Dib-Hajj, S.D., Cohen, S., Hinson, A.W. and Waxman, S.G. Glial cells have heart: rHl Na+ channel mRNA and protein in spinal cord astrocytes. Glia 23: 200-208, 1998.
36. Black, J.A., Dib-Hajj, S.D., McNabola, K., Jeste, S., Rizzo, M.A., is Kocsis, J.D. and Waxman; S.G. Spinal sensory neurons express multiple sodium channel alpha-subunit mRNAs. Mol. Brain Res., 43: 117-131, 1996.
37. Dib-Hajj, S.D., Tyrell, L., Black, J.A. and Waxman, S.G. NaN, a novel voltage-gated Na channel, is expressed preferentially in peripheral 2o sensory neurons and down-regulated after axotomy. Proc. Natl. Acad.
Sci. USA. , 95: 8963-8968, 1998.
38. Yang, J.S.J., Sladky, J.T., Kallen, R.G. and Barchi, R.L. TTX
sensitive and TTX -insensitive sodium-channel messenger-RNA transcripts are independently regulated in adult skeletal-muscle after denervation.
2s Neuron, 7: 421-427, 1991.
39. Fidler, LJ. Tumor heterogeneity and the biology of cancer invasio n and metastasis. Cancer Res., 38: 2651-2660, 1978.
40. Fidler, I.J. and Hart, I.R. Biological diversity in metastatic neoplasms: origins ands implications. Science, 217: 998-1003, 1982.

4l . Plummer, N. W . , McBurney, M . W . and Meisler, M . H . Alternative splicing of the sodium channel SCNBA predicts a truncated two-domain protein in fetal brain and non-neuronal cells. J. Biol. Chem., 272: 24008-24015, 1997.
s 42. Blandino, J.K.W., Viglione, M.P., Bradley, W.A., Oie, H.K., Kim, Y.I. Vpltage-dependent sodium-channels in human small-cell lung-cancer cells - Role in action-potentials and inhibition by Lambert-Eaton syndrome IgG. J. Membr. Biol., 143: 153-163, 1995.
43. Patt, S., Wlasak, R. and Kraft, R. Influence of voltage-activated to sodium channels on growth and motility of human neuroblastoma cells in vitro. Brain Pathol., 10: 738, 2000.
44. Patt, S., Labrakakis, C., Bernstein, M., Weydt, P., CervosNavarro, J. , Nisch, G. , Kettenmann, H. Neuron-like physiological properties of cells from human oligodendroglial tumors. Neurosci., 71: 60I-611, 1996.
is 45. Mansi, J.L., Gogas, H., Bliss, J.M., Gazet, J.C., Bergen U. and Coombes, R.C. Outcome of primary-breast-cancer patients with micrometastases: a long-term follow-up study. The Lancet, 354: 197-202, 1999.
46. Jongsma, H.J. Sudden cardiac death: A matter of faulty ion channels?
2o Curr. Biol. , 8: 8568-8571, 1998.
47. Hardy, S.P., deFelipe, C., Valverde, M.A. Inhibition of voltage-gated cationic channels in rat embryonic hypothalamic neurones and 01300 neuroblastoma cells by triphenylethylene antooestrogens. FEBS
Lett 434: 236-240, 1998.
2s 48. Cushman, M., Costantino, J.P., Tracy, R.P., Song, K., Buckley, L., Roberts, J.D., D.N. Tamoxifen and cardiac risk factors in healthy women: suggestion of an anti-inflammatory effect. Thromb Vasc Biol 21:
255-261, 2001.

Example 2: Design of antisense oligonucleotides for suppressing VGSC expression in human prostate cancer 1. Alignment of all currently known VGSC types to identify potential s sites for VGSC subtype-specific antisense oligonucleotide design.
Cons agtgagtgtgaaagtcttatggagagcaacaaaactg---tccgatggaaa hNav2.1 agtcggtgtgaaagccttctgt---ttaacgaatcca---tgctatgggaa hNe-Na tccgaatgttttgcccttatgaATGTTAGTCAAAATG---TGCGAtggaaa Human brainactgattgcctaaaactaatagaaagaaatgagactg---ctcgatggaaa Human brainagtgagtgcaAAGCTCTCA'TTGAGAGCAATcaaactg---ccaggtggaaa Human brainagtgactgtc--aggctcttggcaagcaa-------g---ctcggtggaaa Na6 (human)actgaatgtgaaaagcttatggaggggAACAATACAGAGATCAGATGgaag hSkMl aacaagtctgagtgcgagagCCTCATGCACACAGGCCAGGtccgctggctc Human heartaacaagagccagtgtgagtccttgaacttgaccggagaattgtactggacc PN3/SNS aacaagtccgagtgtcacaatcaaaacagcaccggccacttcttctgggtc (rat) HNeNa is derived from SCN9A and Na6 from SCNBA.
to In the above alignment the human VGSC equivalent has been used where possible. The alignment has been optimised by the introduction of sequence gaps indicated by a dash although gaps are not actually present in the real sequence or any oligonucleotide design. The most commonly 15 occurring nucleotides are indicated in the consensus line (Cons). Potential sites for the design of ZOmer antisense oligonucleotides are in bold case and underlined in four human VGSC types. The most unconserved region of the fragment produced by the degenerate screen has been used to produce this line-up.
It would also be possible to design 20mer antisense oligos in the 3/4 cytoplasmic linker (where VGSC sequence is highly conserved across all types) that are individually capable of 'silencing' simultaneously a number of VGSC types. For example, below the same 20 nucleotide sections of the 3/4 linker from three VGSC types are shown aligned. In this section, the hNe-Na and the human brain 2 sequences are identical and the hSkM1 sequence differs at only two nucleotide positions. Therefore, in this s region it is possible to design two antisense oligonucleotides that will knock-out at least three of the channels .(possibly four when the Na6 (human) sequence has been confirmed for this region).
hNe-Na TTATGACAGAAGAACAGAAG
Human brain 2 TTATGACAGAAGAACAGAAG
hSkMl TTATGACgGAgGAACAGAAG

Claims (69)

1. A method of determining the susceptibility of a human patient to cancer comprising the steps of (i) obtaining a sample containing nucleic acid and/or protein from the patient; and (ii) determining, whether the sample contains a level of SCN5A voltage-gated Na+ channel nucleic acid or protein associated with cancer.

2. A method of diagnosing cancer in a human patient comprising the steps of (i) obtaining a sample containing nucleic acid and/or protein from the patient; and (ii) determining whether the sample contains a level of SCN5A voltage-gated Na+ channel nucleic acid or protein associated with cancer.

3. A method of predicting the relative prospects of a particular outcome of a cancer in a human patient comprising the steps of (i) obtaining a sample containing nucleic acid and/or protein from the patient;
and (ii) determining whether the sample contains a level of SCN5A
voltage-gated Na+ channel nucleic acid or protein associated with cancer.

4. A method of determining the susceptibility of a human patient to breast cancer comprising the steps of (i) obtaining a sample containing nucleic acid and/or protein from the patient; and (ii) determining whether the sample contains a level of voltage-gated Na+ channel nucleic acid or protein associated with cancer.

5. A method of diagnosing breast cancer in a human patient comprising the steps of (i) obtaining a sample containing nucleic acid and/or protein from the patient; and (ii) determining whether the sample contains a level of voltage-gated Na+ channel nucleic acid or protein associated with cancer.

6. A method of predicting the relative prospects of a particular outcome of a breast cancer in a human patient comprising the steps of (i) obtaining a sample containing nucleic acid and/or protein from the patient; and (ii) determining whether the sample contains a level of voltage-gated Na+
channel nucleic acid or protein associated with cancer.

7. A method according to any one of Claims 1 to 3 wherein the cancer is breast cancer.

8. A method according to any one of the preceding claims wherein the cancer is metastatic.

9. A method according to any one of the preceding claims wherein the method comprises the step of determining whether the sample contains a level of SCN5A voltage-gated Na+ channel nucleic acid or protein associated with cancer.

10. A method according to any of the preceding claims wherein the sample contains nucleic acid and the level of said voltage-gated Na+
channel nucleic acid is measured by contacting the said nucleic acid with a nucleic acid which hybridises selectively to said voltage-gated Na+
channel nucleic acid.

11. A method according to Claim 10 wherein the sample contains mRNA and the nucleic acid as said selectively hybridises to the said voltage-gated Na+ channel mRNA.

12. A method according to Claim 10 or 11 wherein the nucleic acid which hybridises as said is detestably labelled.

13. A method according to any one of Claims 10 to 12 wherein the nucleic acid which selectively hybridises as said is single stranded.

14. A method according to any one of Claims 10 to 13 wherein the nucleic acid which selectively hybridises as said is suitable for use in a nucleic acid amplification reaction.

15. A method according to any one of Claims 1 to 9 wherein the sample contains protein and the level of said voltage-gated Na+ channel protein is measured.

16. A method according to Claim 15 wherein the level of said protein is measured by contacting the protein with a molecule which selectively binds to the said voltage-gated Na+ channel protein.

17. A method according to Claim 16 wherein the selective binding molecule is an antibody or fragment or derivative thereof or an antibody-like molecule.

18. A method according to Claim 16 or 17 wherein the selective binding molecule comprises a detectable label.

19. A method according to any one of the preceding claims wherein the sample is a sample of the tissue in which cancer is suspected or in which cancer may be or has been found, or contains cells from said tissue.

20. A method according to Claim 19 wherein the tissue is breast tissue and the cancer is breast cancer.

21. A method according to Claim 19 wherein the sample is any one of blood, serum or lymphatic circulation and the cancer is breast cancer.

22. Use of an agent which is capable of use in determining the level of SCN5A voltage-gated Na+ channel protein or nucleic acid in a sample in the manufacture of a reagent for; in a method of; or in, diagnosing, prognosing or determining susceptibility to cancer.

23. Use of an agent which is capable of use in determining the level of a voltage-gated Na+ channel protein or nucleic acid in a sample in the manufacture of a reagent for; in a method of; or in, diagnosing, prognosing or determining susceptibility to breast cancer.

24. Use of an agent which is an agent useful in selectively assaying the activity of SCN5A voltage-gated Na+ channel protein in a sample in the manufacture of a reagent for; in a method of; or in, diagnosing cancer or prognosing cancer or determining susceptibility to cancer.

25. Use of an agent which is an agent useful in selectively assaying the activity of a voltage-gated Na+ channel protein in a sample in the manufacture of a reagent for; in a method of; or in, diagnosing or prognosing or determining susceptibility to breast cancer.

26. Use according to any one of Claims 22 to 23 wherein the agent is a nucleic acid which selectively hybridises to the said voltage-gated Na+
channel nucleic acid.

27. Use according to any one of Claims 22 to 25 wherein the agent is a molecule which selectively binds to the said voltage-gated Na+ channel protein.

28. The use according to any one of claims 22 to 27 wherein the voltage-gated Na+ channel is SCN5A.

29. A kit of parts useful for diagnosing cancer comprising an agent which is capable of use in determining the level of SCN5A (and optionally SCN9A) VGSC protein or nucleic acid in a sample.

30. A kit of parts useful for diagnosing breast cancer, comprising (1) an agent which is capable of use in determining the level of VGSC protein or nucleic acid in a sample, and (2) means for separating breast cells from a sample in order to carry out said VGSC assay.

31. A kit of parts according to Claim 29 or 30 further comprising a negative control and/or a positive control.

32. A kit of parts according to any one of Claims 29 or 31 further comprising means for separating breast cells from a sample.

33. A method of treating cancer comprising the step of administering to the patient an agent which selectively prevents the function of SCN5A
voltage-gated Na+ channel.

34. A method of treating breast cancer comprising the step of administering to the patient an agent which selectively prevents the function of a voltage-gated Na+ channel.

35. The method of claim 33 or 34 wherein the voltage-gated Na+ channel is SCN5A.

36. A method according to any one of claims 33 to 35 wherein the agent prevents the expression of the said voltage-gated Na+ channel.

37. A method according to any one of claims 33 to 35 wherein the agent inhibits the activity of the said voltage-gated Na+ channel.

38. A method according to Claim 36 wherein the agent is an antisense molecule.

39. A method according to Claim 36 wherein the agent is a ribozyme.

40. Use of an agent which selectively prevents the function of SCN5A
voltage-gated Na+ channel in the manufacture of a medicament for treating cancer.

41. Use of an agent which selectively prevents the function of a voltage-gated Na+ channel in the manufacture of a medicament for treating breast cancer.

42. A genetic construct comprising a nucleic acid encoding a molecule capable of preventing the function of SCN5A voltage-gated Na+ channel expressed in a cell.

43. A genetic construct according to Claim 42 adapted for delivery to a human cell.

44. A genetic construct according to Claim 43 wherein the adaptation allows delivery to a cancer cell.

45. A genetic construct according to Claim 43 or 44 comprising means to selectively deliver the nucleic acid to a cancer cell.

46. A genetic construct according to any one of Claims 42 to 45 comprising means to selectively express the nucleic acid encoding a molecule as said in a cancer cell.

47. A genetic construct comprising a nucleic acid encoding a molecule capable of preventing the function of a voltage-gated Na+ channel expressed in a cell, wherein expression of said molecule by the genetic construct is via a breast-selective promoter, or wherein the genetic construct is adapted for selective delivery to a breast cell.

48. A genetic construct according to any one of Claims 42 to 47 for use in medicine.

49. A pharmaceutical composition comprising a genetic constrict according to any one of Claims 42 to 37 and a pharmaceutically acceptable carrier.

50. Use of a genetic construct according to any one of Claims 42 to 37 in the manufacture of a medicament for the treatment of cancer.

51. A method of identifying a compound which selectively inhibits a SCN5A voltage-gated Na+ channel, the method comprising (a) contacting a test compound with any one of the said voltage-gated Na+ channels and determining whether said compound is inhibitory; (b) contacting the test compound with other voltage-gated Na+ channels and determining whether said compound is inhibitory; and (c) selecting a compound which is substantially inhibitory in (a) but is not substantially inhibitory in (b).

52. A compound comprising a moiety which selectively binds SCN5A
voltage-gated Na+ channel protein and a further moiety.

53. A compound according to claim 52 wherein the further moiety is a directly or indirectly cytotoxic moiety.

54. A compound according to claim 52 wherein the further moiety is a readily detectable moiety.

55. A compound according to Claim 52 wherein the moiety which selectively binds the said voltage-gated Na+channel protein and the further moiety are polypeptides which are fused.

56. A nucleic acid molecule encoding a compound according to Claim 55.

57. A host cell comprising a nucleic acid molecule according to Claim 56.

58. A compound according to any one of Claims 52 to 55 for use in medicine.

59. A pharmaceutical composition comprising a compound according to any one of Claims 52 to 55 and a pharmaceutically acceptable carrier.

60. Use of a compound according to any one of Claims 52 to 55 in the manufacture of a medicament for the treatment and/or diagnosis of a human patient with or at risk of cancer.

61. A method of treating cancer, the method comprising administering to the human patient an effective amount of a compound according to any one of Claims 52, 53 or 55 wherein the further moiety of the compound is one which either directly or indirectly is of therapeutic benefit to the patient.

62. A method of imaging cancer in a human patient, comprising administering to the patient an effective amount of a compound according to Claim 54.

63. Use of a compound comprising a moiety which selectively binds a voltage-gated Na+channel protein and a further moiety in the manufacture of a medicament for the treatment and/or diagnosis of a human patient with or at risk of breast cancer.

64. A method of treating breast cancer, the method comprising administering to the human patient an effective amount of a compound comprising a moiety which selectively binds a voltage-gated Na+channel protein and a further moiety, wherein the further moiety of the compound is one which either directly or indirectly is of therapeutic benefit to the patient.

65. A method of imaging breast cancer in a human patient, comprising administering to the patient an effective amount of a compound comprising a moiety which selectively binds a voltage-gated Na+channel protein and a further moiety, wherein the further moiety of the compound is one which comprises a readily detectable moiety.

66. A kit of parts comprising (1) a compound according to Claim 53 wherein the further moiety is a cytotoxic moiety which is able to convert a relatively non-toxic prodrug into a cytotoxic drug and (2) a relatively non-toxic prodrug.

67. A kit of parts comprising (1) a compound according to Claim 52 wherein the further moiety is able to bind selectively to a directly or indirectly cytotoxic moiety or to a readily detectable moiety and (2) any one of a directly or indirectly cytotoxic moiety or a readily detectable moiety to which the further moiety of the compound is able to bind.

68. Any novel method of treating or diagnosing cancer or breast cancer as herein disclosed.

69. Any novel compound or composition for use in treating or diagnosing cancer or breast cancer as herein disclosed.