CA2474108A1 - Novel fluorescent proteins from aequorea coerulscens and methods for using same - Google Patents
Novel fluorescent proteins from aequorea coerulscens and methods for using same Download PDFInfo
- Publication number
- CA2474108A1 CA2474108A1 CA002474108A CA2474108A CA2474108A1 CA 2474108 A1 CA2474108 A1 CA 2474108A1 CA 002474108 A CA002474108 A CA 002474108A CA 2474108 A CA2474108 A CA 2474108A CA 2474108 A1 CA2474108 A1 CA 2474108A1
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acid
- peptide
- cell
- detecting
- labeling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
Abstract
The present invention provides nucleic acid compositions encoding a novel colorless GFP-like protein, acGFP, from Aequorea coerulscens and fluorescent and non-fluorescent mutants and derivatives thereof, as well as peptides and proteins encoded by these nucleic acid compositions. The subject protein and nucleic acid compositions of the present invention are colored and/or fluorescent and/or can be photoactivated, and can be used in a variety of different biological applications, particularly for labeling. Finally, kits for use in such biological applications are provided.
Claims (32)
1. An isolated nucleic acid which encodes an Aequorea coerulescens peptide, selected from:
(a) a nucleic acid which encodes a protein comprising an amino acid sequence SEQ ID NO: 02;
(b) a nucleic acid comprising a nucleotide sequence SEQ ID NO: 01.
(a) a nucleic acid which encodes a protein comprising an amino acid sequence SEQ ID NO: 02;
(b) a nucleic acid comprising a nucleotide sequence SEQ ID NO: 01.
2. An isolated nucleic acid selected from the group consisting of:
(a) a nucleic acid derived from the nucleic acid of claim 1 using at least one of site-directed mutagenesis and/or random mutagenesis;
(b) a nucleic acid coding for an amino acid sequence selected from SEQ ID NOs: 04, 06, 08, 10, 12, 14, 16, 18, 20, 22, or 24; or (c) a nucleic acid comprising a nucleotide sequence selected from SEQ ID NOs: 03, 05, 07, 09, 11, 13, 15, 17, 19, 21, or 23.
(d) a nucleic acid differing from the nucleic acid of (a) above due to degeneracy of the genetic code
(a) a nucleic acid derived from the nucleic acid of claim 1 using at least one of site-directed mutagenesis and/or random mutagenesis;
(b) a nucleic acid coding for an amino acid sequence selected from SEQ ID NOs: 04, 06, 08, 10, 12, 14, 16, 18, 20, 22, or 24; or (c) a nucleic acid comprising a nucleotide sequence selected from SEQ ID NOs: 03, 05, 07, 09, 11, 13, 15, 17, 19, 21, or 23.
(d) a nucleic acid differing from the nucleic acid of (a) above due to degeneracy of the genetic code
3. An isolated nucleic acid of claim 2, wherein said nucleic acid encodes a fluorescent protein
4. An expression cassette comprising (a) the nucleic acid of claim 2; and (b) regulatory elements necessary for expression of the nucleic acid in the cell.
5. An expression cassette comprising (a) the nucleic acid of claim 3; and (b) regulatory elements necessary for expression of the nucleic acid in the cell.
6. A cell, or progeny thereof, comprising the expression cassette of claim 4.
7. A cell, or progeny thereof, comprising the expression cassette of claim 5.
8. An isolated peptide encoded by the nucleic acid of claim 2.
9. An antibody binding specifically to the peptide of claim 8.
10. A fusion peptide incorporating the peptide of claim 8.
11. A transgenic organism capable of expressing the peptide of claim 8.
12. A method for labeling or detecting a biological molecule comprising coupling said biological molecule to the peptide of claim 8.
13. A method for labeling or detecting a cell or cell organelle comprising production of the peptide of claim 8 in the cell.
14. A method for detecting a gene expression comprising production of the peptide of claim 8 in the cell.
15. An isolated peptide encoded by the nucleic acid of claim 3.
16. An antibody binding specifically to the peptide of claim 15.
17. A fusion peptide incorporating the peptide of claim 15.
18. A transgenic organism capable of expressing the peptide of claim 15.
19. A method for labeling or detecting a biological molecule comprising coupling said biological molecule to the peptide of claim 15.
20. A method for labeling or detecting a cell or cell organelle comprising production of the peptide of claim 15 in the cell.
21. A method for detecting a gene expression comprising production of the peptide of claim 15 in the cell.
22. A nucleic acid fragment, wherein said fragment is selected from the group consisting of:
(a) a fragment encoding a peptide of at least 100 amino acid residues in length from SEQ ID NOs: 02, 04, 06, 08, 10, 12, 14, 16, 18, 20, 22, or 24; or (b) a fragment substantially similar or identical to a nucleotide sequence of at least 300 residues in length from SEQ ID NO: 01, 03, 05, 07, 09, 11, 13, 15, 17, 19, 21, or 23.
(a) a fragment encoding a peptide of at least 100 amino acid residues in length from SEQ ID NOs: 02, 04, 06, 08, 10, 12, 14, 16, 18, 20, 22, or 24; or (b) a fragment substantially similar or identical to a nucleotide sequence of at least 300 residues in length from SEQ ID NO: 01, 03, 05, 07, 09, 11, 13, 15, 17, 19, 21, or 23.
23. A nucleic acid molecule comprising a nucleic acid fragment of claim 22, wherein the nucleic acid encodes a fluorescent protein
24. An expression cassette comprising (a) the nucleic acid molecule of claim 23; and (b) regulatory elements necessary for expression of the nucleic acid fragment in the cell.
25. A cell, or progeny thereof, comprising the expression cassette of claim 24.
26. An isolated peptide encoded by the nucleic acid fragment of claim 23.
27. An antibody binding specifically to the peptide of claim 26.
28. A fusion peptide incorporating the peptide of claim 26.
29. A transgenic organism capable of expressing the peptide of claim 26.
30. A method for labeling or detecting a biological molecule comprising coupling said biological molecule to the peptide claim 26.
31. A method for labeling or detecting a cell or cell organelle comprising production of the peptide of claim 26 in the cell.
32. A method for detecting a gene expression comprising production of the peptide of claim 26 in a cell.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US35151802P | 2002-01-22 | 2002-01-22 | |
| US60/351,518 | 2002-01-22 | ||
| PCT/IB2003/000907 WO2003062270A2 (en) | 2002-01-22 | 2003-01-17 | Fluorescent protein from aequorea coerulscens and uses therof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2474108A1 true CA2474108A1 (en) | 2003-07-31 |
| CA2474108C CA2474108C (en) | 2012-06-19 |
Family
ID=27613506
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2474108A Expired - Lifetime CA2474108C (en) | 2002-01-22 | 2003-01-17 | Novel fluorescent proteins from aequorea coerulscens and methods for using same |
Country Status (12)
| Country | Link |
|---|---|
| US (4) | US7432053B2 (en) |
| EP (1) | EP1485481B1 (en) |
| JP (3) | JP4510464B2 (en) |
| AT (1) | ATE458816T1 (en) |
| AU (1) | AU2003208520B2 (en) |
| CA (1) | CA2474108C (en) |
| DE (1) | DE60331422D1 (en) |
| DK (1) | DK1485481T3 (en) |
| ES (1) | ES2340154T3 (en) |
| IL (2) | IL162568A0 (en) |
| RU (1) | RU2330886C2 (en) |
| WO (1) | WO2003062270A2 (en) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1485481B1 (en) * | 2002-01-22 | 2010-02-24 | Evrogen IP | Fluorescent protein from aequorea coerulescens and uses thereof |
| EP1576157B1 (en) | 2002-12-26 | 2010-07-21 | Zakrytoe Aktsionernoe Obschestvo " Evrogen" | Fluorescent proteins from copepoda species and methods for using same |
| AU2005311680A1 (en) | 2004-12-01 | 2006-06-08 | Proteologics, Inc. | Ubiquitin ligase assays and related reagents |
| UA93922C2 (en) * | 2009-03-20 | 2011-03-25 | Михаил Викторович Разуменко | Process for production of peptides, specifically recognizing the cells of particular type, meant for therapeutic goals |
| US8435961B2 (en) | 2009-06-26 | 2013-05-07 | Massachusetts Institute Of Technology | Methods and compositions for increasing the activity of inhibitory RNA |
| US8268550B2 (en) * | 2009-06-26 | 2012-09-18 | Massachusetts Institute Of Technology | Compositions and methods for identification of PARP function, inhibitors, and activators |
| US20110097329A1 (en) | 2009-06-26 | 2011-04-28 | Massachusetts Institute Of Technology | Compositions and methods for treating cancer and modulating stress granule formation |
| KR101800878B1 (en) * | 2010-03-31 | 2017-11-27 | 전남대학교 산학협력단 | Methods for Analyzing a Genotoxicity Using Non-fluorescent Fluorescence Proteins |
| WO2011135040A1 (en) | 2010-04-30 | 2011-11-03 | F. Hoffmann-La Roche Ag | Fluorescent antibody fusion protein, its production and use |
| US9134301B2 (en) * | 2010-11-22 | 2015-09-15 | Bio-Rad Laboratories, Inc. | Sorting of adherent cells by selective transformation of labels |
| JP6388575B2 (en) | 2012-06-06 | 2018-09-12 | アメリカ合衆国 | Kit for detecting and monitoring endocrine disrupting substances (EDC) |
| US20140024066A1 (en) * | 2012-06-20 | 2014-01-23 | California Institute Of Technology | Animal model having photo-activatable mitochondria |
| RU2602452C2 (en) * | 2014-05-14 | 2016-11-20 | Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук | Method of substances and their compositions detection with anticancer activity |
| JPWO2022215532A1 (en) * | 2021-04-07 | 2022-10-13 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5625048A (en) * | 1994-11-10 | 1997-04-29 | The Regents Of The University Of California | Modified green fluorescent proteins |
| US5777079A (en) * | 1994-11-10 | 1998-07-07 | The Regents Of The University Of California | Modified green fluorescent proteins |
| US5668750A (en) * | 1995-07-28 | 1997-09-16 | Nec Corporation | Bipolar multiplier with wide input voltage range using multitail cell |
| US5874304A (en) * | 1996-01-18 | 1999-02-23 | University Of Florida Research Foundation, Inc. | Humanized green fluorescent protein genes and methods |
| US6096865A (en) * | 1996-05-06 | 2000-08-01 | Amgen Inc. | Mutants of the green fluorescent protein having improved fluorescent properties at 37° |
| WO2000034326A1 (en) * | 1998-12-11 | 2000-06-15 | Clontech Laboratories, Inc. | Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof |
| EP1341861B1 (en) * | 2000-12-01 | 2006-02-15 | 3M Innovative Properties Company | Crosslinked pressure sensitive adhesive compositons, and adhesive articles based thereon, useful in high temperature applications |
| GB0109858D0 (en) * | 2001-04-23 | 2001-06-13 | Amersham Pharm Biotech Uk Ltd | Fluorscent proteins |
| EP1485481B1 (en) | 2002-01-22 | 2010-02-24 | Evrogen IP | Fluorescent protein from aequorea coerulescens and uses thereof |
-
2003
- 2003-01-17 EP EP03706812A patent/EP1485481B1/en not_active Expired - Lifetime
- 2003-01-17 CA CA2474108A patent/CA2474108C/en not_active Expired - Lifetime
- 2003-01-17 DK DK03706812.9T patent/DK1485481T3/en active
- 2003-01-17 AT AT03706812T patent/ATE458816T1/en not_active IP Right Cessation
- 2003-01-17 JP JP2003562147A patent/JP4510464B2/en not_active Expired - Fee Related
- 2003-01-17 AU AU2003208520A patent/AU2003208520B2/en not_active Expired
- 2003-01-17 US US10/501,629 patent/US7432053B2/en not_active Expired - Lifetime
- 2003-01-17 RU RU2004125585/13A patent/RU2330886C2/en active
- 2003-01-17 DE DE60331422T patent/DE60331422D1/en not_active Expired - Lifetime
- 2003-01-17 ES ES03706812T patent/ES2340154T3/en not_active Expired - Lifetime
- 2003-01-17 IL IL16256803A patent/IL162568A0/en unknown
- 2003-01-17 WO PCT/IB2003/000907 patent/WO2003062270A2/en active Application Filing
-
2004
- 2004-06-16 IL IL162568A patent/IL162568A/en not_active IP Right Cessation
-
2008
- 2008-08-28 US US12/200,113 patent/US7879988B2/en not_active Expired - Lifetime
- 2008-08-28 US US12/200,064 patent/US7897726B2/en active Active
- 2008-08-28 US US12/199,983 patent/US7667016B2/en not_active Expired - Lifetime
-
2009
- 2009-10-22 JP JP2009243614A patent/JP2010046084A/en active Pending
-
2010
- 2010-11-05 JP JP2010248679A patent/JP5465649B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| IL162568A0 (en) | 2005-11-20 |
| US20090148940A1 (en) | 2009-06-11 |
| US20090137033A1 (en) | 2009-05-28 |
| US7897726B2 (en) | 2011-03-01 |
| ES2340154T3 (en) | 2010-05-31 |
| WO2003062270A8 (en) | 2004-11-04 |
| WO2003062270A2 (en) | 2003-07-31 |
| US20090148939A1 (en) | 2009-06-11 |
| JP5465649B2 (en) | 2014-04-09 |
| AU2003208520B2 (en) | 2008-06-05 |
| US7667016B2 (en) | 2010-02-23 |
| US7879988B2 (en) | 2011-02-01 |
| JP2011087581A (en) | 2011-05-06 |
| DE60331422D1 (en) | 2010-04-08 |
| ATE458816T1 (en) | 2010-03-15 |
| JP2010046084A (en) | 2010-03-04 |
| JP4510464B2 (en) | 2010-07-21 |
| CA2474108C (en) | 2012-06-19 |
| US7432053B2 (en) | 2008-10-07 |
| EP1485481B1 (en) | 2010-02-24 |
| DK1485481T3 (en) | 2010-06-07 |
| EP1485481A2 (en) | 2004-12-15 |
| JP2005526495A (en) | 2005-09-08 |
| IL162568A (en) | 2010-12-30 |
| WO2003062270B1 (en) | 2004-04-01 |
| WO2003062270A3 (en) | 2003-11-27 |
| RU2330886C2 (en) | 2008-08-10 |
| US20060167225A1 (en) | 2006-07-27 |
| RU2004125585A (en) | 2005-04-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20230117 |