CA2483697A1 - Nanoparticle probes with raman spectroscopic fingerprints for analyte detection - Google Patents

Nanoparticle probes with raman spectroscopic fingerprints for analyte detection Download PDF

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Publication number
CA2483697A1
CA2483697A1 CA002483697A CA2483697A CA2483697A1 CA 2483697 A1 CA2483697 A1 CA 2483697A1 CA 002483697 A CA002483697 A CA 002483697A CA 2483697 A CA2483697 A CA 2483697A CA 2483697 A1 CA2483697 A1 CA 2483697A1
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substrate
raman
particle
oligonucleotides
bound
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French (fr)
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CA2483697C (en
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Chad Mirkin
Yun-Wei Cao
Rongchao Jin
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Northwestern University
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587—Nanoparticles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/583—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with non-fluorescent dye label
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention encompasses reagents comprising particles with at least one Raman dye and a specific binding members bound thereto and methods of using such reagents. The invention also encompasses reagents of a specific binding member and two or more different Raman dyes and methods for using such reagents. New types of particle probes having a specific binding member bound thereto are described. These reagents are used in a novel detection strategy that utilizes the catalytic properties of the Au nanoparticles to generate a silver coating that can behave as a surface-enhanced Raman scattering (SERS) promoter for the dye-labeled particles that have been captured by target and an underlying chip in microarray format. The strategy provides the high sensitivity and high selectivity attributes of grey-scale scanometric detection but provides a route to multiplexing and ratioing capabilities since a very large number of probes can be designed based upon the concept of using a Raman tag as a spectroscopic fingerprint in detection. These spectra are used as fingerprints to differentiate oligonucleotide or other targets in one solution. This method has been used to distinguish six dissimilar DNA targets with six Raman labeled nanoparticle probes, and also two RNA targets with single nucleotide polymorphisms (SNPs).

Claims (88)

1. A reagent comprising a particle having bound there to at least one Raman label and a specific binding member wherein the Raman label can be activated to provide a SERS effect.
2. The reagent of claim 1 wherein the particle has two or more different Raman labels.
3. The reagent of claim 1 wherein the specific binding member is a DNA, RNA, polypeptide, antibody, antigen, carbohydrate, protein, peptide, amino acid, carbohydrate, hormone, steroid, vitamin, drug, virus, polysaccharides, lipids, lipopolysaccharides, glycoproteins, lipoproteins, nucleoproteins, oligonucleotides, antibodies, immunoglobulins, albumin, hemoglobin, coagulation factors, peptide and protein hormones, non-peptide hormones, interleukins, interferons, cytokines, peptides comprising a tumor-specific epitope, cells, cell-surface molecules, microorganisms, fragments, portions, components or products of microorganisms, small organic molecules, nucleic acids and oligonucleotides, metabolites of or antibodies to any of the above substances.
4. The reagent of claim 3 wherein nucleic acids and oligonucleotides comprise genes, viral RNA and DNA, bacterial DNA, fungal DNA, mammalian DNA, cDNA, mRNA, RNA and DNA fragments, oligonucleotides, synthetic oligonucleotides, modified oligonucleotides, single-stranded and double-stranded nucleic acids, natural and synthetic nucleic acids.
5. The reagent of claim 1 wherein the particle is a gold, Ag, Cu, Pt, Ag/Au, Pt/Au, Cu/Au coreshell and alloy particles.
6. The reagent of claim 1 wherein the particle is a nanoparticle.
7. The reagent of claim 1 wherein the particle is a metallic nanoparticle.
8. The reagent of claim 1 wherein the particle is a gold nanoparticle.
9. The reagent of claim 1 wherein the Raman label is directly bound to the particle.
10. The reagent of claim 9 wherein the Raman label is indirectly bound to the particle.
11. The reagent of claim 1 wherein at least some of the Raman labels are conjugated to the specific binding pair member.
12. The reagent of claim 11 wherein the conjugate is covalently bound to the particle.
13. The reagent of claim 1 wherein the specific binding pair member is covalently bound to the particle via a functional group.
14. The reagent of claim 1 wherein the Raman label is activated by a staining material.
15. The reagent of claim 1 wherein the staining material comprises silver, gold or copper stain.
16. A reagent comprising a specific binding member having two or more different Raman labels bound thereto.
17. The reagent of claim 16 wherein the specific binding member comprises a member of a specific binding pair selected from the group consisting of antigen and antibody-specific binding pairs, biotin and avidin binding pairs, carbohydrate and lectin bind pairs, complementary nucleotide sequences, complementary peptide sequences, effector and receptor molecules, enzyme cofactor and enzymes, and enzyme inhibitors and enzymes.
18. The reagent of claim 16 wherein the specific binding member is a DNA, RNA, polypeptide, antibody, antigen, carbohydrate, protein, peptide, amino acid, carbohydrate, hormone, steroid, vitamin, drug, virus, polysaccharides, lipids, lipopolysaccharides, glycoproteins, lipoproteins, nucleoproteins, oligonucleotides, antibodies, immunoglobulins, albumin, hemoglobin, coagulation factors, peptide and protein hormones, non-peptide hormones, interleukins, interferons, cytokines, peptides comprising a tumor-specific epitope, cells, cell-surface molecules, microorganisms, fragments, portions, components or products of microorganisms, small organic molecules, nucleic acids and oligonucleotides, metabolites of or antibodies to any of the above substances.
19. The reagent of claim 18 wherein nucleic acids and oligonucleotides comprise genes, viral RNA and DNA, bacterial DNA, fungal DNA, mammalian DNA, cDNA, mRNA, RNA and DNA fragments, oligonucleotides, synthetic oligonucleotides, modified oligonucleotides, single-stranded and double-stranded nucleic acids, natural and synthetic nucleic acids.
20. A reagent of claim 16 wherein the specific binding member is a DNA, RNA, antibody, antigen, polypeptide or carbohydrate.
21. A method for detecting an analyte comprising:
(a) forming a complex of the reagent of claim 1 and the analyte;
(b) binding the complex to a substrate;
(c) staining the complex on the substrate to activate the SERS effect in the Raman label; and (d) measuring the SERS effect.
22. The method of claim 21 wherein the complex is bound to the substrate through one or more specific binding substances.
23. A method for detecting an analyte comprising:
(a) binding the analyte to a substrate;
(b) complexing the reagent of claim 1 with the analyte on the substrate (c) staining the complex on the substrate to activate the SERS effect in the Raman label;
(d) measuring the SRS effect.
24. The method of claim 23 wherein the reagent is indirectly bound to the analyte on the substrate through one or more specific binding substances.
25. The method of claim 21 or 23 wherein the target analyte comprises an antibody, an antigen, a hapten, a receptor, a ligand, a protein, a peptide, a polypeptide, a nucleic acid, a membrane or membrane fraction, a lipid, a membrane-protein complex, a carbohydrate, a virus, a cell or macromolecule or molecular complex.
26. The method of claim 21 or 23 wherein the specific binding member comprises an antibody, an antigen, a receptor, a ligand, a protein, a polypeptide, small molecule or a nucleic acid.
27. The method of claims 21 or 23 wherein the specific binding member comprises a member of a specific binding pair selected from the group consisting of antigen and antibody-specific binding pairs, biotin and avidin binding pairs, carbohydrate and lectin bind pairs, complementary nucleotide sequences, complementary peptide sequences, effector and receptor molecules, enzyme cofactor and enzymes, and enzyme inhibitors and enzymes.
28. The method of claim 27 wherein the specific binding member is a DNA, RNA, polypeptide, antibody, antigen, carbohydrate, protein, peptide, amino acid, carbohydrate, hormone, steroid, vitamin, drug, virus, polysaccharides, lipids, lipopolysaccharides, glycoproteins, lipoproteins, nucleoproteins, oligonucleotides, antibodies, immunoglobulins, albumin, hemoglobin, coagulation factors, peptide and protein hormones, non-peptide hormones, interleukins, interferons, cytokines, peptides comprising a tumor-specific epitope, cells, cell-surface molecules, microorganisms, fragments, portions, components or products of microorganisms, small organic molecules, nucleic acids and oligonucleotides, metabolites of or antibodies to any of the above substances.
29. The method of claim 28 wherein nucleic acids and oligonucleotides comprise genes, viral RNA and DNA, bacterial DNA, fungal DNA, mammalian DNA, cDNA, mRNA, RNA and DNA fragments, oligonucleotides, synthetic oligonucleotides, modified oligonucleotides, single-stranded and double-stranded nucleic acids, natural and synthetic nucleic acids.
30. The method of claims 21 or 23 wherein the substrate has a plurality of different first specific binding members attached thereto in an array to allow for the detection of multiple types of target analytes.
31. The method of claims 21 or 23 wherein the substrate comprises a glass slide, microplate well, beads, polymer membrane, or optical fiber.
32. A method for detecting for the presence or absence of one or more target analytes in a sample, the target analytes having at least two binding sites, said method comprising:
providing a substrate having bound thereto one or more types of a first specific binding member for immobilizing the target analytes onto said substrate;
providing one or more types of particles, each type of particles having bound thereto (a) one or more Raman labels; and (b) a second specific binding member for binding to a specific target analyte, wherein (i) the Raman active labels bound to each type of particle is different and serves as an identifier for a specific target analyte; and (ii) the second specific binding member bound to each type of particle is different and is targeted to a specific target analyte;
contacting the particles, the sample and the substrate under conditions effective for specific binding interactions between the target analyte and first and second specific binding member so as to form a test substrate having particles complexed thereto in the presence of one or more target analytes in the sample;
contacting the test substrate with a staining material to produce a detection substrate having a surface capable of causing surface-enhanced Raman scattering (SERS);
and determining for the presence of said particle complexes on said detection substrate as an indication of the presence of one or more target analytes in the sample by obtaining and analyzing a SERS spectrum.
33. The method of claim 32 wherein the substrate has a plurality of different first specific binding members attached thereto in an array to allow for the detection of multiple types of target analytes.
34. The method of claim 32 wherein the substrate comprises a glass slide, microplate well, beads, polymer membrane, or optical fiber.
35. The method of claim 32 wherein at least a portion of the Raman labels is conjugated to the specific binding member.
36. The method of claim 32 wherein the Raman labels is conjugated to an oligonucleotide.
37. The method of claim 33 wherein the oligonucleotide is a polyadenosine or polythymidine.
38. The method of claim 32 wherein the oligonucleotide is not complementary to any target nucleic acid.
39. The method of claim 32 wherein the particle is a nanoparticle.
40. The method of claim 32 wherein the particle is a metallic nanoparticle.
41. The method of claim 32 wherein the Raman label is conjugated to the specific binding pair member.
42. The method of claim 41 wherein the conjugate is covalently bound to the particle.
43. The method of claim 32 wherein the specific binding member is a DNA, RNA, polypeptide, antibody, antigen, carbohydrate, protein, peptide, amino acid, carbohydrate, hormone, steroid, vitamin, drug, virus, polysaccharides, lipids, lipopolysaccharides, glycoproteins, lipoproteins, nucleoproteins, oligonucleotides, antibodies, immunoglobulins, albumin, hemoglobin, coagulation factors, peptide and protein hormones, non-peptide hormones, interleukins, interferons, cytokines, peptides comprising a tumor-specific epitope, cells, cell-surface molecules, microorganisms, fragments, portions, components or products of microorganisms, small organic molecules, nucleic acids and oligonucleotides, metabolites of or antibodies to any of the above substances.
44. The method of claim 43 wherein nucleic acids and oligonucleotides comprise genes, viral RNA and DNA, bacterial DNA, fungal DNA, mammalian DNA (e.g., human DNA), cDNA, mRNA, RNA and DNA fragments, oligonucleotides, synthetic oligonucleotides, modified oligonucleotides, single-stranded and double-stranded nucleic acids, natural and synthetic nucleic acids.
45. The reagent of claim 32 wherein the specific binding member comprises a member of a specific binding pair selected from the group consisting of antigen and antibody-specific binding pairs, biotin and avidin binding pairs, carbohydrate and lectin bind pairs, complementary nucleotide sequences, complementary peptide sequences, effector and receptor molecules, enzyme cofactor and enzymes, and enzyme inhibitors and enzymes.
46. A method for detecting for the presence or absence of one or more target nucleic acids in a sample, the sequence of the nucleic acid having at least two portions, said method comprising:

providing a substrate having oligonucleotides bound thereto, the oligonucleotides bound to the substrate having a sequence that is complementary to a first portion of a specific nucleic acid target;
providing one or more types of particles, each type of particles comprising oligonucleotides and at least one or more Raman active labels bound thereto, wherein (i) at least some of the oligonucleotides attached to each type of particle have a sequence that is complementary to a second portion of the sequence of a specific target nucleic acid; and (ii) the Raman labels bound to each type of particles is different and serves as an identifier for a specific target nucleic acid, said Raman label comprising at least one Raman label.
contacting the particles, the substrate, and the sample under conditions effective for hybridization of the oligonucleotides bound to the substrate with the first portion of the nucleic acid and for hybridization of the oligonucleotides attached to the particle with the second portion of the nucleic acid so as to form a test substrate having one or more particle complexes bound thereto when one or more target nucleic acids are present in said sample;
contacting the test substrate with a staining material to produce a detection substrate having a surface capable of causing surface-enhanced Raman scattering (SERS);
and determining for the presence of said particle complexes on said detection substrate as an indication of the presence of one or more target nucleic acids in the sample by obtaining and analyzing a SERS spectrum.
47. The method according to claim 46 wherein the Raman label is conjugated to at least a portion of the oligonucleotides.
48. The method of claim 46 wherein the substrate has a plurality of different first specific binding members attached thereto in an array to allow for the detection of multiple types of target analytes.
49. The method of claim 46 wherein the substrate comprises a glass slide, microplate well, beads, polymer membrane, or optical fiber.
50. The method of claim 46 wherein at least a portion of the Raman labels is conjugated to the specific binding member.
51. The method of claim 46 wherein the Raman labels is conjugated to an oligonucleotide.
52. The method of claim 51 wherein the oligonucleotide is a polyadenosine or polythymidine.
53. The method of claim 51 wherein the oligonucleotide is not complementary to any target nucleic acid.
54. The reagent of claim 46 wherein the particle is a nanoparticle.
55. The reagent of claim 46 wherein the particle is a metallic nanoparticle.
56. The reagent of claim 46 wherein the Raman label is conjugated to the specific binding pair member.
57. The reagent of claim x wherein the conjugate is covalently bound to the particle.
58. A method for detecting for the presence or absence of one or more target nucleic acids in a sample, the sequence of the nucleic acid having at least two portions, said method comprising:
providing a substrate having oligonucleotides bound thereto, the oligonucleotides bound to the substrate having a sequence that is complementary to a first portion of the nucleic acid;
providing one or more types of particles, each type of particles comprising oligonucleotides bound thereto and a Raman label bound to at least a portion of the oligonucleotides, wherein (i) at least some of the oligonucleotides attached to the particle have a sequence that is complementary to a second portion of the nucleic acid;
and (ii) the Raman labels bound to each type of particles serves as an identifier for a specific target nucleic acid, said Raman label comprising at least one Raman label providing a detectable or measurable Raman scattering signal when illuminated by radiation capable of inducing a Raman scattering;
contacting the particles, the substrate, and the sample under conditions effective for hybridization of the oligonucleotides bound to the substrate with the first portion of the nucleic acid and for hybridization of the oligonucleotides attached to the particle with the second portion of the nucleic acid so as to form a test substrate having a particle complex bound thereto when said target nucleic acid is present in said sample;
contacting the test substrate with a staining material to produce a detection substrate having a surface capable of causing surface-enhanced Raman scattering (SERS);
and determining for the presence of said particle complex on said detection substrate as an indication of the presence of the target nucleic acid in the sample by obtaining and analyzing a SERS spectrum.
59. The method according to claim 58 wherein the Raman label is conjugated to at least a portion of the oligonucleotides.
60. The method of claim 58 wherein the substrate has a plurality of different oligonucleotides attached thereto in an array to allow for the detection of multiple types of target nucleic acids or portions of a target nucleic acid.
61. The method of claim 58 wherein the substrate comprises a glass slide, microplate well, beads, polymer membrane, or optical fiber.
62. The method of claim 58 wherein the particle is a nanoparticle.
63. The method of claim 58 wherein the particle is a metallic nanoparticle.
64. The method of claim 63 wherein the particle is a gold nanoparticle.
65. A method for screening one or more molecules to determine whether the molecule is a ligand to one or more specific receptors, the molecules are present in a sample, said method comprising:
providing a substrate having bound thereto one or more specific receptors;
providing one or more types of conjugates comprising particles, oligonucleotides bound to the particles, a Raman active label bound to a portion of the oligonucleotides, and the molecule from said sample bound to a portion of the oligonucleotides of a specific type of conjugate, wherein said Raman active label comprising at least one Raman active molecule providing a detectable or measurable Raman scattering signal when illuminated by radiation capable of inducing a Raman scattering;
contacting the particles, sample and substrate under conditions effective for specific binding interactions between the molecule bound to the particles with the specific receptor bound to the substrate so as to form a test substrate having particles complexed thereto when the molecule is a ligand to a specific receptor;
contacting the test substrate with a staining material to produce a detection substrate having a surface capable of causing surface-enhanced Raman scattering (SERS);
and determining for the presence of said particle complexes on said detection substrate as a confirmation of a ligand to a specific receptor by obtaining and analyzing a SERS
spectrum.
66. The method of claim 65 wherein the substrate has a plurality of different receptors attached thereto in an array to allow for the detection of multiple types of molecules.
67. The method of claim 65 wherein the substrate comprises a glass slide, microplate well, beads, polymer membrane, or optical fiber.
68. The method of claim 65 wherein the particle is a nanoparticle.
69. The method of claim 65 wherein the particle is a metallic nanoparticle.
70. The method of claim 69 wherein the particle is a gold nanoparticle.
71. A method for screening one or more molecules to determine whether the molecule is a ligand to one or more specific receptors, the molecules are present in a sample, said method comprising:
providing a substrate having bound thereto one or more specific receptors;
providing a molecule modified with a first member of a specific binding pair;
providing one or more types of conjugates, each type of conjugate comprising a particle, one or more Raman active labels bound to the particle, and a second member of the specific binding pair bound to the particle, wherein said Raman active label comprising at least one Raman active molecule providing a detectable or measurable Raman scattering signal when illuminated by radiation capable of inducing a Raman scattering;
contacting the particles, sample and substrate under conditions effective for specific binding interactions between the molecule with the specific receptor bound to the substrate and between the first and second members of the specific binding pair so as to form a test substrate having particles complexed thereto when the molecule is a ligand to a specific receptor;
contacting the test substrate with a staining material to produce a detection substrate having a surface capable of causing surface-enhanced Raman scattering (SERS);
and determining for the presence of said particle complexes on said detection substrate as a confirmation of a ligand to a specific receptor by obtaining and analyzing a SERS
spectrum.

71. The method of claim 70 wherein the substrate has a plurality of different receptors attached thereto in an array to allow for the detection of multiple types of molecules.
72. The method of claim 70 wherein the substrate comprises a glass slide, microplate well, beads, polymer membrane, or optical fiber.
73. The method of claim 70 wherein the particle is a gold, Ag, Cu, Pt, Ag/Au, Pt/Au, Cu/Au coreshell and alloy particles.
74. The method of claim 70 wherein the particle is a nanoparticle.
75. The method of claim 70 wherein the particle is a metallic nanoparticle.
76. The method of claim 75 wherein the particle is a gold nanoparticle.
77. The method of claim 70 wherein the Raman label is directly bound to the particle.
78. The method of claim 70 wherein the Raman label is indirectly bound to the particle.
79. The method of claim 70 wherein the Raman label is activated by a staining material.
80. The method of claim 79 wherein the staining material comprises silver, gold or copper stain.
81. A test kit comprising the reagent of claim 1 in one container and a Raman enhancer stain in another container.
82. A test kit comprising the reagent of claim 1 in one container and a silver, gold or copper stain Raman enhancer in another container.
83. A test kit comprising the reagent of claim 16 in one container and a Raman enhancer stain in another container.
84. A test kit comprising the reagent of claim 16 in one container and a silver, gold or copper stain Raman enhancer in another container.
85. A fiber optic detection device comprising a bundle of optical fibers terminating with ends of the optical fiber wherein a plurality of the optical fibers have a reagent of claim 1 located at the ends of the optical fiber.
86. The fiber optic detection device of claim 85 wherein two or more of the reagents of claim 1 at the ends of the optical fiber have different specific binding member and different Raman labels.
87. A fiber optic detection device comprising a bundle of optical fibers terminating with ends of the optical fiber wherein a plurality of the optical fibers have a reagent of claim 16 located at the ends of the optical fiber.
88. The fiber optic detection device of claim 85 wherein two or more of the reagents of claim 16 at the ends of the optical fiber have different specific binding member and different Raman labels.
CA2483697A 2002-05-07 2003-05-07 Nanoparticle probes with raman spectroscopic fingerprints for analyte detection Expired - Fee Related CA2483697C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US37853802P 2002-05-07 2002-05-07
US60/378,538 2002-05-07
US38363002P 2002-05-28 2002-05-28
US60/383,630 2002-05-28
US10/172,428 US20030211488A1 (en) 2002-05-07 2002-06-14 Nanoparticle probs with Raman spectrocopic fingerprints for analyte detection
US10/172,428 2002-06-14
PCT/US2003/014100 WO2003095973A2 (en) 2002-05-07 2003-05-07 Nanoparticle probes with raman spectroscopic fingerprints for analyte detection

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CA2483697C CA2483697C (en) 2010-03-30

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EP (1) EP1499889B1 (en)
JP (1) JP2005524849A (en)
AT (1) ATE515698T1 (en)
AU (1) AU2003237180B2 (en)
CA (1) CA2483697C (en)
WO (1) WO2003095973A2 (en)

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WO2003095973A2 (en) 2003-11-20
AU2003237180A1 (en) 2003-11-11
US20040086897A1 (en) 2004-05-06
AU2003237180B2 (en) 2007-05-24
US20030211488A1 (en) 2003-11-13
US7985539B2 (en) 2011-07-26
EP1499889A2 (en) 2005-01-26
CA2483697C (en) 2010-03-30
JP2005524849A (en) 2005-08-18
ATE515698T1 (en) 2011-07-15

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