CA2519760A1 - Cbh1 homologs and variant cbh1 cellulases - Google Patents
Cbh1 homologs and variant cbh1 cellulases Download PDFInfo
- Publication number
- CA2519760A1 CA2519760A1 CA002519760A CA2519760A CA2519760A1 CA 2519760 A1 CA2519760 A1 CA 2519760A1 CA 002519760 A CA002519760 A CA 002519760A CA 2519760 A CA2519760 A CA 2519760A CA 2519760 A1 CA2519760 A1 CA 2519760A1
- Authority
- CA
- Canada
- Prior art keywords
- variant
- cbh
- cbh1
- protein
- detergent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- CHGCMTXJJDMXNF-SJZSRGDHSA-N CC1(C2)C2([C@@H](CC2)C3)[C@@H]2CC3C1 Chemical compound CC1(C2)C2([C@@H](CC2)C3)[C@@H]2CC3C1 CHGCMTXJJDMXNF-SJZSRGDHSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
Abstract
Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A
(formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A
wherein one or more amino acid residues are substituted and/or deleted.
(formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A
wherein one or more amino acid residues are substituted and/or deleted.
Claims (25)
1. An isolated cellulase protein selected from the group consisting of a CBH1 from Hypocrea schweinitzii, Hypocrea orientalis, Trichoderma pseudokoningii and Trichoderma konilangbra.
2. The isolated cellulase protein of Claim 1 wherein said protein is the CBH1 from Hypocrea orientalis comprising the amino acid sequence according to SEQ ID
NO:5.
NO:5.
3. The isolated cellulase protein of Claim 1 wherein said protein is the CBH1 from Hypocrea schweinitzii comprising the amino acid sequence according to SEQ ID
NO:8.
NO:8.
4. The isolated cellulase protein of Claim 1 wherein said protein is the CBH1 from Trichoderma konilangbra comprising the amino acid sequence according to SEQ
ID NO:11.
ID NO:11.
5. The isolated cellulase protein of Claim 1 wherein said protein is the CBH1 from Trichoderma pseudokoningii comprising the amino acid sequence according to SEQ ID NO:14.
6. A variant CBH1 cellulase, wherein said variant comprises a substitution or deletion at a position corresponding to one or more of residues L6, P13, T24, Q27, S47, T59, T66, G88, T160, Q186, S195, T232, E236, E239, G242, N250, T281, F311, N327, D329, A336, K354, V407, P412, T417 and/or F418 of the mature H.
jecorina CBH1 protein presented in Figure 1.
jecorina CBH1 protein presented in Figure 1.
7. A variant CBH1 cellulase according to Claim 6, wherein said variant comprises a substitution at a position corresponding to a residue selected from the group consisting of Q186(E), S195(A/F), E239S, G242(H/Y/N/S/T/D/A) and P412(T/S/A).
8. An isolated nucleic acid sequence which encodes or is complementary to a sequence which encodes a CBH1 polypeptide selected from the group consisting of the amino acid sequences presented as SEQ ID NO:5, 8, 11 and 14.
9. An isolated nucleic acid selected from the group consisting of SEQ ID NO:
3, 6, 9 and 12.
3, 6, 9 and 12.
10. An isolated nucleic acid sequence encoding a CBH1 according to Claim 6.
11. A vector comprising a nucleic acid according to claim 8.
12. A vector comprising a nucleic acid according to claim 9.
13. A vector comprising a nucleic acid according to claim 10.
14. A host cell transformed with the vector of claim 11.
15. A host cell transformed with the vector of claim 12.
16. A host cell transformed with the vector of claim 13.
17. A method of producing a CBH I variant comprising the steps of:
(a) culturing the host cell according to claim 14 in a suitable culture medium under suitable conditions to produce CBH I variant;
(b) obtaining said produced CBH I variant.
(a) culturing the host cell according to claim 14 in a suitable culture medium under suitable conditions to produce CBH I variant;
(b) obtaining said produced CBH I variant.
18. A method of producing a CBH I variant comprising the steps of:
(a) culturing the host cell according to claim 15 in a suitable culture medium under suitable conditions to produce CBH I variant;
(b) obtaining said produced CBH I variant.
(a) culturing the host cell according to claim 15 in a suitable culture medium under suitable conditions to produce CBH I variant;
(b) obtaining said produced CBH I variant.
19. A method of producing a CBH I variant comprising the steps of:
(a) culturing the host cell according to claim 16 in a suitable culture medium under suitable conditions to produce CBH I variant;
(b) obtaining said produced CBH I variant.
(a) culturing the host cell according to claim 16 in a suitable culture medium under suitable conditions to produce CBH I variant;
(b) obtaining said produced CBH I variant.
20. A detergent composition comprising a surfactant and a CBH I variant, wherein said CBH I variant comprises a CBH I variant according to claim 1.
21. The detergent according to claim 20, wherein said detergent is a laundry detergent.
22. The detergent according to claim 20, wherein said detergent is a dish detergent.
23. A feed additive comprising a CBH I variant according to claim 1.
24. A method of treating wood pulp comprising contacting said wood pulp with a CBH
I variant according to claim 1.
I variant according to claim 1.
25. A method of converting biomass to sugars comprising contacting said biomass with a CBH I variant according to claim 1.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA2770976A CA2770976C (en) | 2003-03-21 | 2004-03-19 | Cbh1 homologs and variant cbh1 cellulases |
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US45636803P | 2003-03-21 | 2003-03-21 | |
US60/456,368 | 2003-03-21 | ||
US45869603P | 2003-03-27 | 2003-03-27 | |
US60/458,696 | 2003-03-27 | ||
PCT/US2004/008520 WO2005028636A2 (en) | 2003-03-21 | 2004-03-19 | Novel cbh1 homologs and variant cbh1 cellulases |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2770976A Division CA2770976C (en) | 2003-03-21 | 2004-03-19 | Cbh1 homologs and variant cbh1 cellulases |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2519760A1 true CA2519760A1 (en) | 2005-03-31 |
CA2519760C CA2519760C (en) | 2012-05-22 |
Family
ID=34380856
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2770976A Expired - Fee Related CA2770976C (en) | 2003-03-21 | 2004-03-19 | Cbh1 homologs and variant cbh1 cellulases |
CA2519760A Expired - Fee Related CA2519760C (en) | 2003-03-21 | 2004-03-19 | Cbh1 homologs and variant cbh1 cellulases |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2770976A Expired - Fee Related CA2770976C (en) | 2003-03-21 | 2004-03-19 | Cbh1 homologs and variant cbh1 cellulases |
Country Status (6)
Country | Link |
---|---|
US (3) | US7452707B2 (en) |
EP (3) | EP1606392A4 (en) |
JP (2) | JP2007534294A (en) |
CN (1) | CN1898381B (en) |
CA (2) | CA2770976C (en) |
WO (1) | WO2005028636A2 (en) |
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