CA2519760A1 - Cbh1 homologs and variant cbh1 cellulases - Google Patents

Cbh1 homologs and variant cbh1 cellulases Download PDF

Info

Publication number
CA2519760A1
CA2519760A1 CA002519760A CA2519760A CA2519760A1 CA 2519760 A1 CA2519760 A1 CA 2519760A1 CA 002519760 A CA002519760 A CA 002519760A CA 2519760 A CA2519760 A CA 2519760A CA 2519760 A1 CA2519760 A1 CA 2519760A1
Authority
CA
Canada
Prior art keywords
variant
cbh
cbh1
protein
detergent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002519760A
Other languages
French (fr)
Other versions
CA2519760C (en
Inventor
Frits Goedegebuur
Peter Gualfetti
Colin Mitchinson
Paulien Neefe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International, Inc.
Frits Goedegebuur
Peter Gualfetti
Colin Mitchinson
Paulien Neefe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International, Inc., Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe filed Critical Genencor International, Inc.
Priority to CA2770976A priority Critical patent/CA2770976C/en
Publication of CA2519760A1 publication Critical patent/CA2519760A1/en
Application granted granted Critical
Publication of CA2519760C publication Critical patent/CA2519760C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Fodder In General (AREA)
  • Processing Of Solid Wastes (AREA)

Abstract

Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A
(formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A
wherein one or more amino acid residues are substituted and/or deleted.

Claims (25)

1. An isolated cellulase protein selected from the group consisting of a CBH1 from Hypocrea schweinitzii, Hypocrea orientalis, Trichoderma pseudokoningii and Trichoderma konilangbra.
2. The isolated cellulase protein of Claim 1 wherein said protein is the CBH1 from Hypocrea orientalis comprising the amino acid sequence according to SEQ ID
NO:5.
3. The isolated cellulase protein of Claim 1 wherein said protein is the CBH1 from Hypocrea schweinitzii comprising the amino acid sequence according to SEQ ID
NO:8.
4. The isolated cellulase protein of Claim 1 wherein said protein is the CBH1 from Trichoderma konilangbra comprising the amino acid sequence according to SEQ
ID NO:11.
5. The isolated cellulase protein of Claim 1 wherein said protein is the CBH1 from Trichoderma pseudokoningii comprising the amino acid sequence according to SEQ ID NO:14.
6. A variant CBH1 cellulase, wherein said variant comprises a substitution or deletion at a position corresponding to one or more of residues L6, P13, T24, Q27, S47, T59, T66, G88, T160, Q186, S195, T232, E236, E239, G242, N250, T281, F311, N327, D329, A336, K354, V407, P412, T417 and/or F418 of the mature H.
jecorina CBH1 protein presented in Figure 1.
7. A variant CBH1 cellulase according to Claim 6, wherein said variant comprises a substitution at a position corresponding to a residue selected from the group consisting of Q186(E), S195(A/F), E239S, G242(H/Y/N/S/T/D/A) and P412(T/S/A).
8. An isolated nucleic acid sequence which encodes or is complementary to a sequence which encodes a CBH1 polypeptide selected from the group consisting of the amino acid sequences presented as SEQ ID NO:5, 8, 11 and 14.
9. An isolated nucleic acid selected from the group consisting of SEQ ID NO:
3, 6, 9 and 12.
10. An isolated nucleic acid sequence encoding a CBH1 according to Claim 6.
11. A vector comprising a nucleic acid according to claim 8.
12. A vector comprising a nucleic acid according to claim 9.
13. A vector comprising a nucleic acid according to claim 10.
14. A host cell transformed with the vector of claim 11.
15. A host cell transformed with the vector of claim 12.
16. A host cell transformed with the vector of claim 13.
17. A method of producing a CBH I variant comprising the steps of:
(a) culturing the host cell according to claim 14 in a suitable culture medium under suitable conditions to produce CBH I variant;
(b) obtaining said produced CBH I variant.
18. A method of producing a CBH I variant comprising the steps of:
(a) culturing the host cell according to claim 15 in a suitable culture medium under suitable conditions to produce CBH I variant;
(b) obtaining said produced CBH I variant.
19. A method of producing a CBH I variant comprising the steps of:
(a) culturing the host cell according to claim 16 in a suitable culture medium under suitable conditions to produce CBH I variant;
(b) obtaining said produced CBH I variant.
20. A detergent composition comprising a surfactant and a CBH I variant, wherein said CBH I variant comprises a CBH I variant according to claim 1.
21. The detergent according to claim 20, wherein said detergent is a laundry detergent.
22. The detergent according to claim 20, wherein said detergent is a dish detergent.
23. A feed additive comprising a CBH I variant according to claim 1.
24. A method of treating wood pulp comprising contacting said wood pulp with a CBH
I variant according to claim 1.
25. A method of converting biomass to sugars comprising contacting said biomass with a CBH I variant according to claim 1.
CA2519760A 2003-03-21 2004-03-19 Cbh1 homologs and variant cbh1 cellulases Expired - Fee Related CA2519760C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2770976A CA2770976C (en) 2003-03-21 2004-03-19 Cbh1 homologs and variant cbh1 cellulases

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US45636803P 2003-03-21 2003-03-21
US60/456,368 2003-03-21
US45869603P 2003-03-27 2003-03-27
US60/458,696 2003-03-27
PCT/US2004/008520 WO2005028636A2 (en) 2003-03-21 2004-03-19 Novel cbh1 homologs and variant cbh1 cellulases

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2770976A Division CA2770976C (en) 2003-03-21 2004-03-19 Cbh1 homologs and variant cbh1 cellulases

Publications (2)

Publication Number Publication Date
CA2519760A1 true CA2519760A1 (en) 2005-03-31
CA2519760C CA2519760C (en) 2012-05-22

Family

ID=34380856

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2770976A Expired - Fee Related CA2770976C (en) 2003-03-21 2004-03-19 Cbh1 homologs and variant cbh1 cellulases
CA2519760A Expired - Fee Related CA2519760C (en) 2003-03-21 2004-03-19 Cbh1 homologs and variant cbh1 cellulases

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CA2770976A Expired - Fee Related CA2770976C (en) 2003-03-21 2004-03-19 Cbh1 homologs and variant cbh1 cellulases

Country Status (6)

Country Link
US (3) US7452707B2 (en)
EP (3) EP1606392A4 (en)
JP (2) JP2007534294A (en)
CN (1) CN1898381B (en)
CA (2) CA2770976C (en)
WO (1) WO2005028636A2 (en)

Families Citing this family (156)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005028636A2 (en) * 2003-03-21 2005-03-31 Genencor International, Inc. Novel cbh1 homologs and variant cbh1 cellulases
EP2385104A1 (en) 2003-04-01 2011-11-09 Danisco US Inc. Variant Scytalidium thermophilium CBH1
EP2377931B1 (en) 2003-08-25 2013-05-08 Novozymes Inc. Variants of glycoside hydrolases
US7985569B2 (en) 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
DK3219806T3 (en) 2004-03-25 2020-07-13 Novozymes Inc PROCEDURES FOR DECOMPOSITION OR TRANSFORMATION OF PLANT CELL WALL POLYSACCHARIDES
CA2592550C (en) 2004-12-30 2015-05-19 Genencor International, Inc. Novel variant hypocrea jecorina cbh2 cellulases
DK2032703T3 (en) 2006-06-26 2011-06-14 Dsm Ip Assets Bv Transfection of high yield filamentous fungus
CA2693084A1 (en) * 2007-06-08 2008-12-18 Benjamin S. Bower Heterologous and homologous cellulase expression system
WO2009046283A1 (en) * 2007-10-05 2009-04-09 University Of Georgia Research Foundation, Inc. Apparatus and methods for treating biomass
CA2709490A1 (en) 2007-12-19 2009-07-09 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CA2712151A1 (en) * 2008-01-18 2009-07-23 Iogen Energy Corporation Cellulase variants with reduced inhibition by glucose
CN102159707A (en) 2008-08-01 2011-08-17 艾欧基能源公司 Family 6 cellulase with decreased inactivation by lignin
CA2745760A1 (en) 2008-12-04 2010-06-10 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
DK2379712T3 (en) 2008-12-19 2017-05-08 Novozymes Inc Methods for Increasing Hydrolysis of Cellulosic Material in the Presence of Cellobiose Dehydrogenase
WO2010080527A1 (en) 2008-12-19 2010-07-15 Novozymes, Inc. Methods for determining cellulolytic enhancing activity of a polypeptide
CN102325893A (en) 2008-12-19 2012-01-18 诺维信股份有限公司 Methods for increasing enzymatic hydrolysis of cellulosic material in the presence of a peroxidase
WO2010080407A2 (en) 2008-12-19 2010-07-15 Novozymes, Inc. Methods for increasing hydrolysis of cellulosic material
US8604277B2 (en) 2009-01-28 2013-12-10 Novozymes, Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
WO2010088463A2 (en) 2009-01-30 2010-08-05 Novozymes, Inc. Polypeptides having expansin activity and polynucleotides encoding same
WO2010096931A1 (en) 2009-02-27 2010-09-02 Iogen Energy Corporation Novel lignin-resistant cellulase enzymes
US8658409B2 (en) 2009-03-24 2014-02-25 Novozymes A/S Polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
AU2010253848C1 (en) 2009-05-29 2015-02-19 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
ES2534078T3 (en) 2009-06-02 2015-04-17 Novozymes Inc. Polypeptides with cellobiohydrolase activity and polynucleotides encoding them
CA2767169A1 (en) 2009-07-07 2011-01-13 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2011035027A2 (en) 2009-09-17 2011-03-24 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN102712916B (en) 2009-09-18 2015-11-25 诺维信股份有限公司 There are the polypeptide of beta-glucosidase activity and the polynucleotide of this polypeptide of coding
DK3296394T3 (en) 2009-09-23 2020-12-21 Danisco Us Inc STATUS UNKNOWN GLYCOSYL HYDROLASE ENZYMES AND USES
CN102648276A (en) 2009-09-29 2012-08-22 诺维信股份有限公司 Polypeptides having xylanase activity and polynucleotides encoding same
ES2560805T3 (en) 2009-09-29 2016-02-22 Novozymes Inc. Polypeptides with cellulolytic enhancing activity and polynucleotides that encode them
EP2977382A3 (en) 2009-09-30 2016-05-11 Novozymes Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN102666846A (en) 2009-09-30 2012-09-12 诺维信公司 Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2011050037A1 (en) 2009-10-23 2011-04-28 Novozymes, Inc. Cellobiohydrolase variants and polynucleotides encoding same
US20120260371A1 (en) 2009-10-29 2012-10-11 Novozymes A/S Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same
EP2496693B1 (en) 2009-11-06 2017-10-25 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
CN102639697B (en) 2009-11-06 2015-03-25 诺维信股份有限公司 Polypeptides having xylanase activity and polynucleotides encoding same
AU2010333801B2 (en) 2009-12-23 2015-12-17 Danisco Us Inc. Methods for improving the efficiency of simultaneous saccharification and fermentation reactions
DK2357227T3 (en) 2010-02-11 2015-09-14 Süd Chemie Ip Gmbh & Co Kg Optimized cellulase enzymes
BR112012020804A2 (en) 2010-02-11 2015-09-15 Iogen Energy Corp reduced lignin binding carbohydrate binding modules
FR2957922A1 (en) * 2010-03-26 2011-09-30 Inst Francais Du Petrole NEW CBH1-EG1 FUSION PROTEINS AND THEIR USE
US8828701B2 (en) 2010-03-31 2014-09-09 Novozymes, Inc. Cellobiohydrolase variants and polynucleotides encoding same
JP2011234683A (en) * 2010-05-12 2011-11-24 Ｂｉｏ−ｅｎｅｒｇｙ株式会社 Yeast with enhanced saccharification power of cellulose
DK2588604T3 (en) 2010-06-30 2016-09-26 Novozymes Inc Polypeptides having beta-glucosidase activity and polynucleotides encoding them
US9057086B2 (en) 2010-08-12 2015-06-16 Novozymes, Inc. Compositions comprising a polypeptide having cellulolytic enhancing activity and a bicycle compound and uses thereof
WO2012030811A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US20130212746A1 (en) 2010-08-30 2013-08-15 Novoyzmes A/S Polypeptides Having Hemicellulolytic Activity And Polynucleotides Encoding Same
EP2611901B1 (en) 2010-08-30 2016-05-11 Novozymes A/S Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same
WO2012030849A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
EP2611914A1 (en) 2010-08-30 2013-07-10 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US9267126B2 (en) 2010-08-30 2016-02-23 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
JP2013539971A (en) * 2010-09-15 2013-10-31 ザ・リージェンツ・オブ・ザ・ユニバーシティ・オブ・カリフォルニア Thermophilic mutant of endoglucanase I of TRICHODERMARESEI
CN103282489B (en) 2010-09-30 2016-12-14 诺维信股份有限公司 There is polypeptide variants and the coded polynucleotide thereof of cellulolytic enhancing activity
ES2599613T3 (en) 2010-09-30 2017-02-02 Novozymes, Inc. Variants of polypeptides that have cellulolytic enhancement activity and polynucleotides encoding them
MX2013002586A (en) 2010-10-01 2013-03-21 Novozymes Inc Beta-glucosidase variants and polynucleotides encoding same.
BR112013009817B1 (en) 2010-10-26 2020-02-04 Novozymes As methods to degrade or convert sugar cane refuse, to produce a fermentation product, and to ferment sugar cane refuse
BR112013010008B1 (en) 2010-11-02 2020-09-08 Novozymes, Inc. METHODS FOR DEGRADING AND FERMENTING A CELLULOSIC MATERIAL, AND FOR PRODUCING A FERMENTATION PRODUCT
US9212354B2 (en) 2010-11-04 2015-12-15 Novozymes Inc. Polypeptides having cellobiohydrolase activitiy and polynucleotides encoding same
US9139823B2 (en) 2010-11-12 2015-09-22 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
WO2012068509A1 (en) 2010-11-18 2012-05-24 Novozymes, Inc. Chimeric polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
EP2649188A1 (en) 2010-12-06 2013-10-16 Novozymes North America, Inc. Methods of hydrolyzing oligomers in hemicellulosic liquor
WO2012103288A1 (en) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
MX2013007720A (en) 2011-01-26 2013-08-09 Novozymes As Polypeptides having cellobiohydrolase activity and polynucleotides encoding same.
MX337942B (en) 2011-01-26 2016-03-29 Novozymes As Polypeptides having endoglucanase activity and polynucleotides encoding same.
US9506048B2 (en) 2011-01-26 2016-11-29 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2012103293A1 (en) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
DK2670853T3 (en) 2011-01-31 2017-08-28 Novozymes North America Inc Process for enzymatic refining of pretreated cellulosic material for sugars
EP2678352B1 (en) 2011-02-23 2017-12-06 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN103608461B (en) 2011-03-09 2016-08-03 诺维信公司 The method increasing the cellulolytic enhancing activity of polypeptide
US9409958B2 (en) 2011-03-10 2016-08-09 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
RU2013146245A (en) 2011-03-17 2015-04-27 ДАНИСКО ЮЭс ИНК. METHOD FOR REDUCING VISCOSITY IN THE SUGARING PROCESS
DK2689011T3 (en) 2011-03-25 2018-01-22 Novozymes As PROCEDURE FOR DEGRADATION OR CONVERSION OF CELLULOSE-SUBSTANCING MATERIAL
WO2012135659A2 (en) 2011-03-31 2012-10-04 Novozymes A/S Methods for enhancing the degradation or conversion of cellulosic material
WO2012135719A1 (en) * 2011-03-31 2012-10-04 Novozymes, Inc. Cellulose binding domain variants and polynucleotides encoding same
US9926547B2 (en) 2011-04-28 2018-03-27 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
BR112013027463A2 (en) 2011-04-29 2017-09-26 Novozymes Inc methods for degrading or converting, and for fermenting a cellulolysis material, for producing a fermentation product, and for cleaning or washing a hard surface or dirty laundry, and detergent composition
WO2012159009A1 (en) 2011-05-19 2012-11-22 Novozymes, Inc. Methods for enhancing the degradation of cellulosic material with chitin binding proteins
US20140141471A1 (en) 2011-05-19 2014-05-22 Novozymes, Inc. Methods for Enhancing the Degradation of Cellulosic Material with Chitin Binding Proteins
US20140147895A1 (en) 2011-07-22 2014-05-29 Novozymes A/S Processes for Pretreating Cellulosic Material and Improving Hydrolysis Thereof
BR112014002401B1 (en) 2011-08-04 2021-08-03 Novozymes A/S FILAMENTAL FUNGUS TRANSGENIC HOST CELL, METHODS FOR PRODUCING A POLYPEPTIDE AND A PROTEIN, AND, NUCLEIC ACID CONSTRUCT OR AN EXPRESSION VECTOR
BR112014002405A2 (en) 2011-08-04 2017-04-04 Novozymes As isolated polypeptide and polynucleotide, composition, nucleic acid construct or expression vector, recombinant host cell, transgenic plant, part of the plant or plant cell, methods for making a polypeptide and a protein, and processes for degrading a cellulosic material, to produce a fermentation product, and to ferment a cellulosic material
BR112014004190A2 (en) 2011-08-24 2017-03-01 Novozymes Inc method for constructing a filamentous fungal strain, filamentous fungal strain, method for producing multiple recombinant polypeptides, and tandem construct
AU2012298713B2 (en) 2011-08-24 2017-11-23 Novozymes, Inc. Methods for obtaining positive transformants of a filamentous fungal host cell
US9279163B2 (en) 2011-08-31 2016-03-08 Iogen Energy Corporation Cellobiohydrolase enzymes
CN103930555A (en) 2011-09-13 2014-07-16 诺维信北美公司 Methods of hydrolyzing and fermenting cellulosic material
WO2013043910A1 (en) 2011-09-20 2013-03-28 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN103930438A (en) 2011-09-30 2014-07-16 诺维信股份有限公司 Chimeric polypeptides having beta-glucosidase activity and polynucleotides encoding same
EP2773656B1 (en) 2011-10-31 2019-06-19 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
BR112014011902A2 (en) 2011-11-18 2017-05-16 Novozymes As isolated polypeptide and polynucleotide, recombinant host cell, methods for making a polypeptide, and for producing a parent cell mutant, processes for making a protein, for degrading or converting a cellulosic material or xylan-containing material, for producing a fermentation product, and to ferment a cellulosic material or xylan-containing material, and, integral broth formulation or cell culture composition
EP2802651B1 (en) 2011-11-21 2017-03-08 Novozymes, Inc. Gh61 polypeptide variants and polynucleotides encoding same
DK2782998T3 (en) 2011-11-22 2018-04-16 Novozymes Inc POLYPEPTIDES WITH BETA-XYLOSIDASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM
EP2785732B1 (en) 2011-12-01 2017-04-12 Novozymes, Inc. Polypeptides having beta-xylosidase activity and polynucleotides encoding same
EP3272862A1 (en) 2011-12-16 2018-01-24 Novozymes, Inc. Polypeptides having laccase activity and polynucleotides encoding same
EP2794870A4 (en) 2011-12-19 2015-06-17 Novozymes Inc Polypeptides having catalase activity and polynucleotides encoding same
WO2013096369A1 (en) 2011-12-19 2013-06-27 Novozymes A/S Processes and compositions for increasing the digestibility of cellulosic materials
CA2878019A1 (en) 2011-12-20 2013-06-27 Novozymes, Inc. Cellobiohydrolase variants and polynucleotides encoding same
EP2794899A1 (en) 2011-12-21 2014-10-29 Novozymes, Inc. Methods for determining the degradation of a biomass material
US9394556B2 (en) 2012-04-23 2016-07-19 Novozymes A/S Polypeptides having glucuronyl esterase activity and polynucleotides encoding same
CN104245929A (en) 2012-04-23 2014-12-24 诺维信公司 Polypeptides having alpha-glucuronidase activity and polynucleotides encoding same
CN113234695A (en) 2012-04-27 2021-08-10 诺维信股份有限公司 GH61 polypeptide variants and polynucleotides encoding same
KR101572009B1 (en) 2012-09-05 2015-11-25 아프레시아 파마슈티칼스 컴퍼니 Three-dimensional printing system and equipment assembly
BR112015005884A2 (en) 2012-09-19 2017-08-08 Novozymes Inc E Novozymes As processes for degradation of a cellulosic material, for production of a fermentation product, and fermentation of a cellulosic material, composition, and whole broth formulation or cell culture composition
EP2903412B1 (en) 2012-10-08 2019-09-11 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US20150275194A1 (en) 2012-10-24 2015-10-01 Novozymes A/S Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same
KR20150093729A (en) * 2012-12-12 2015-08-18 다니스코 유에스 인크. Variants of cellobiohydrolases
US20160122735A1 (en) 2012-12-12 2016-05-05 Danisco Us Inc. Variants of cellobiohydrolases
MX2015007225A (en) 2012-12-12 2015-10-29 Danisco Us Inc Variants of cellobiohydrolases.
US9938551B2 (en) 2012-12-12 2018-04-10 Danisco Us Inc Variants of cellobiohydrolases
KR20150093739A (en) * 2012-12-12 2015-08-18 다니스코 유에스 인크. Variants of cellobiohydrolases
WO2014093835A1 (en) 2012-12-14 2014-06-19 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2014099798A1 (en) 2012-12-19 2014-06-26 Novozymes A/S Polypeptides having cellulolytic enhancinc activity and polynucleotides encoding same
CN105164254B (en) 2013-03-08 2019-06-28 诺维信公司 Cellobiohydrolase variant and the polynucleotides for encoding them
US9850512B2 (en) 2013-03-15 2017-12-26 The Research Foundation For The State University Of New York Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
US9951363B2 (en) 2014-03-14 2018-04-24 The Research Foundation for the State University of New York College of Environmental Science and Forestry Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
CN104560922B (en) * 2014-03-25 2018-04-27 中国科学院青岛生物能源与过程研究所 A kind of cellulose restriction endonuclease Cel7B mutant of improved performance
US11390898B2 (en) 2014-09-05 2022-07-19 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
EP3191587B1 (en) 2014-09-08 2019-07-17 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
EP4001419A1 (en) 2015-01-28 2022-05-25 DSM IP Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
US10287610B2 (en) 2015-01-28 2019-05-14 Dsm Ip Assets B.V. Integrated process for coproducing alcohol and organic acid from lignocellulosic material
WO2016120298A1 (en) 2015-01-28 2016-08-04 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2016138167A2 (en) 2015-02-24 2016-09-01 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
EP3067428A1 (en) 2015-03-12 2016-09-14 BETA RENEWABLES S.p.A. A process for producing a hydrolyzed mixture from a pre-treated ligno-cellulosic slurry comprising a slurry liquid and slurry solids
WO2016169893A1 (en) 2015-04-20 2016-10-27 Dsm Ip Assets B.V. Whole fermentation broth
WO2016169892A1 (en) 2015-04-20 2016-10-27 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2016207144A1 (en) 2015-06-22 2016-12-29 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
US20180148683A1 (en) 2015-07-07 2018-05-31 Danisco Us Inc. Induction of gene expression using a high concentration sugar mixture
CN105018446B (en) * 2015-08-06 2017-11-14 南京天凯生物技术股份有限公司 The thinner of syrup in cellulose exoglucanase CBH I mutant and Corn Fuel Ethanol fermenting and producing
AU2016310470A1 (en) 2015-08-21 2018-02-22 Aprecia Pharmaceuticals LLC Three-dimensional printing system and equipment assembly
CN109415712A (en) 2016-03-02 2019-03-01 诺维信公司 Cellobiohydrolase variant and the polynucleotides for encoding them
DK3433358T3 (en) 2016-03-24 2022-09-26 Novozymes As Cellobiohydrolase variants and polynucleotides encoding them
EP3469090A1 (en) 2016-06-09 2019-04-17 DSM IP Assets B.V. Seed train for large scale enzyme production
US10913938B2 (en) 2016-07-29 2021-02-09 Dsm Ip Assets B.V. Polypeptides having cellulolytic enhancing activity and uses thereof
WO2018053058A1 (en) 2016-09-14 2018-03-22 Danisco Us Inc. Lignocellulosic biomass fermentation-based processes
US10876103B2 (en) 2016-10-04 2020-12-29 Danisco Us Inc Protein production in filamentous fungal cells in the absence of inducing substrates
US20190276809A1 (en) 2016-11-24 2019-09-12 Dsm Ip Assets B.V. Enzyme composition
BR112019010355B1 (en) 2016-11-24 2024-03-05 Dsm Ip Assets B.V. ENZYME COMPOSITION
US20190330577A1 (en) 2016-12-21 2019-10-31 Dupont Nutrition Biosciences Aps Methods of using thermostable serine proteases
WO2018185071A1 (en) 2017-04-03 2018-10-11 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
US11319559B2 (en) 2017-10-09 2022-05-03 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
CA3078156A1 (en) 2017-10-30 2019-05-09 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2019086370A1 (en) 2017-10-30 2019-05-09 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2019185681A1 (en) 2018-03-28 2019-10-03 Dsm Ip Assets B.V. Enzyme composition
WO2019185680A1 (en) 2018-03-28 2019-10-03 Dsm Ip Assets B.V. Enzyme composition
WO2019219804A1 (en) 2018-05-17 2019-11-21 Dsm Ip Assets B.V. Process for producing a polypeptide
CA3099202A1 (en) 2018-05-30 2019-12-05 Dsm Ip Assets B.V. Process for producing sugars from carbohydrate materials
KR20210038591A (en) 2018-07-30 2021-04-07 다니스코 유에스 인크. Mutant and genetically modified filamentous fungal strains comprising an improved protein productivity phenotype and methods thereof
WO2020058248A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
WO2020058253A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
WO2020058249A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
US20210380958A1 (en) 2018-10-24 2021-12-09 Dsm Ip Assets B.V Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
EP3938525A1 (en) 2019-03-12 2022-01-19 DSM IP Assets B.V. Process for producing a fermentation broth
EP4028536A1 (en) 2019-09-10 2022-07-20 DSM IP Assets B.V. Enzyme composition
KR20230004495A (en) 2020-04-22 2023-01-06 다니스코 유에스 인크. Compositions and methods for improved protein production in filamentous fungal cells
WO2022013148A1 (en) 2020-07-13 2022-01-20 Dsm Ip Assets B.V. Process for the production of biogas
WO2022214459A1 (en) 2021-04-06 2022-10-13 Dsm Ip Assets B.V. Enzyme composition
WO2022214458A1 (en) 2021-04-06 2022-10-13 Dsm Ip Assets B.V. Enzyme composition
BR112023020370A2 (en) 2021-04-06 2023-11-21 Dsm Ip Assets Bv ENZYME COMPOSITION
AU2022253636A1 (en) 2021-04-08 2023-06-01 Versalis S.P.A. Process for the preparation of a sugar product and a fermentation product

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1368599A (en) 1970-09-29 1974-10-02 Unilever Ltd Softening compositions
GB1358599A (en) 1970-12-22 1974-07-03 Monacelli Umberto Gun for firing tacks
DK187280A (en) 1980-04-30 1981-10-31 Novo Industri As RUIT REDUCING AGENT FOR A COMPLETE LAUNDRY
GB2094826B (en) 1981-03-05 1985-06-12 Kao Corp Cellulase enzyme detergent composition
GB2095275B (en) 1981-03-05 1985-08-07 Kao Corp Enzyme detergent composition
CA1338400C (en) 1983-08-31 1996-06-18 David H. Gelfand Recombinant fungal cellulases
US4822516A (en) 1986-12-08 1989-04-18 Kao Corporation Detergent composition for clothing incorporating a cellulase
US5776757A (en) 1988-03-24 1998-07-07 Novo Nordisk A/S Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase and method of making thereof
EP0406314B1 (en) 1988-03-24 1993-12-01 Novo Nordisk A/S A cellulase preparation
DK494089D0 (en) 1989-10-06 1989-10-06 Novo Nordisk As
US5246853A (en) 1990-10-05 1993-09-21 Genencor International, Inc. Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I
ATE211773T1 (en) 1990-10-05 2002-01-15 Genencor Int METHODS FOR TREATING COTTON CONTAINING FIBERS WITH CELLULASE
CA2093422C (en) 1990-10-05 2001-04-03 Detergent compositions containing cellulase compositions deficient in cbh i type components
US6268196B1 (en) * 1993-12-17 2001-07-31 Genencor International, Inc. Method and compositions for treating cellulose containing fabrics using truncated cellulase enzyme compositions
GB9400623D0 (en) * 1994-01-14 1994-03-09 Univ Leeds Exploitation of the cellulase enzyme complex of neurospora
US5912157A (en) * 1994-03-08 1999-06-15 Novo Nordisk A/S Alkaline cellulases
US6184019B1 (en) * 1995-10-17 2001-02-06 Röhm Enzyme Finland OY Cellulases, the genes encoding them and uses thereof
WO1997018286A1 (en) * 1995-11-15 1997-05-22 Novo Nordisk A/S A process for combined desizing and 'stone-washing' of dyed denim
AU4221397A (en) * 1996-09-13 1998-04-02 Meiji Seika Kaisha Ltd. Regulatory sequence of cellulase cbh1 genes originating in (trichoderma viride) and system for mass-producing proteins or peptides therewith
US6265204B1 (en) 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
JP4307563B2 (en) 1997-04-07 2009-08-05 ユニリーバー・ナームローゼ・ベンノートシャープ Agrobacterium-mediated transformation of filamentous fungi, especially those belonging to the genus Aspergillus
US7375197B2 (en) * 2002-01-14 2008-05-20 Midwest Research Institute Cellobiohydrolase I gene and improved variants
AU6210800A (en) * 1999-07-13 2001-01-30 Midwest Research Institute Cellobiohydrolase reduced glycosylation variants: cbhin45a; cbhin270a; and cbhin384a
AU2002346656A1 (en) * 2001-12-06 2003-06-23 Prodigene, Inc. Methods for the cost-effective saccharification of lignocellulosic biomass
EP2305800B1 (en) * 2002-08-16 2015-05-27 Danisco US Inc. Novel variant hyprocrea jecorina CBH1 cellulases
WO2005028636A2 (en) * 2003-03-21 2005-03-31 Genencor International, Inc. Novel cbh1 homologs and variant cbh1 cellulases
EP2385104A1 (en) * 2003-04-01 2011-11-09 Danisco US Inc. Variant Scytalidium thermophilium CBH1

Also Published As

Publication number Publication date
CA2519760C (en) 2012-05-22
US7452707B2 (en) 2008-11-18
WO2005028636A3 (en) 2006-08-17
CN1898381B (en) 2013-07-17
CN1898381A (en) 2007-01-17
CA2770976C (en) 2015-05-19
US20050054039A1 (en) 2005-03-10
EP1606392A2 (en) 2005-12-21
EP1606392A4 (en) 2007-10-17
JP2011152136A (en) 2011-08-11
US20110177561A1 (en) 2011-07-21
US8765440B2 (en) 2014-07-01
EP2298876A1 (en) 2011-03-23
US7951570B2 (en) 2011-05-31
JP2007534294A (en) 2007-11-29
CA2770976A1 (en) 2005-03-31
US20090163397A1 (en) 2009-06-25
WO2005028636A8 (en) 2005-05-06
EP2311942A1 (en) 2011-04-20
WO2005028636A2 (en) 2005-03-31

Similar Documents

Publication Publication Date Title
CA2519760A1 (en) Cbh1 homologs and variant cbh1 cellulases
EP1862540B1 (en) Novel trichoderma genes
JP5148288B2 (en) Novel mutant hypocrea jecorina CBH2 cellulase
Saloheimo et al. A novel, small endoglucanase gene, egl5, from Trichoderma reesei isolated by expression in yeast
CA2520636A1 (en) Variant humicola grisea cbh1.1
CN103289976B (en) The neomorph of Hypocrea jecorina CBH1 cellulase
JP2012528598A (en) Cellulase variants having improved expression, activity, and stability, and methods of use thereof
CA2833029A1 (en) Treatment of cellulosic material and enzymes useful therein
US8871479B2 (en) Cellulase Cel5H related reagents and their use in microorganisms
JP3962805B2 (en) Protein having cellulase activity and method for producing the same
Murashima et al. Determination of subunit composition of Clostridium cellulovorans cellulosomes that degrade plant cell walls
Toda et al. Gene cloning of an endoglucanase from the basidiomycete Irpex lacteus and its cDNA expression in Saccharomyces cerevisiae
JP5771920B2 (en) Thermostable cellobiohydrolase and use thereof
EP1764411A1 (en) Alkaline xylanase
JP5434689B2 (en) Cellulase complex and use thereof
CA2994320A1 (en) Treatment of cellulosic material and enzymes useful therein
JP4257979B2 (en) Improved heat-resistant endoglucanase and its gene
JP4784874B2 (en) Improved heat-resistant endoglucanase and its gene
EP3133155B1 (en) Hyperthermostable endoglucanase
Bélaich et al. Genes and proteins involved in cellulose degradation by mesophilic Clostridia
Gorsche et al. 16 Trichoderma as Cell Factories
Ljungdahl¹ et al. CELLULOSOMES OF ANAEROBIC FUNGI
Ferrer et al. Cellulases from rumen

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20180319