CA2565523A1 - Electrochemical assay device and related methods - Google Patents
Electrochemical assay device and related methods Download PDFInfo
- Publication number
- CA2565523A1 CA2565523A1 CA002565523A CA2565523A CA2565523A1 CA 2565523 A1 CA2565523 A1 CA 2565523A1 CA 002565523 A CA002565523 A CA 002565523A CA 2565523 A CA2565523 A CA 2565523A CA 2565523 A1 CA2565523 A1 CA 2565523A1
- Authority
- CA
- Canada
- Prior art keywords
- side walls
- reagent
- electrode
- bpy
- opening
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/403—Cells and electrode assemblies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/004—Enzyme electrodes mediator-assisted
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
- Y10T156/10—Methods of surface bonding and/or assembly therefor
- Y10T156/1052—Methods of surface bonding and/or assembly therefor with cutting, punching, tearing or severing
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
- Y10T156/10—Methods of surface bonding and/or assembly therefor
- Y10T156/1052—Methods of surface bonding and/or assembly therefor with cutting, punching, tearing or severing
- Y10T156/1062—Prior to assembly
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
- Y10T156/10—Methods of surface bonding and/or assembly therefor
- Y10T156/1052—Methods of surface bonding and/or assembly therefor with cutting, punching, tearing or severing
- Y10T156/1062—Prior to assembly
- Y10T156/1074—Separate cutting of separate sheets or webs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T29/00—Metal working
- Y10T29/49—Method of mechanical manufacture
- Y10T29/49002—Electrical device making
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T29/00—Metal working
- Y10T29/49—Method of mechanical manufacture
- Y10T29/49002—Electrical device making
- Y10T29/49108—Electric battery cell making
- Y10T29/49114—Electric battery cell making including adhesively bonding
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T29/00—Metal working
- Y10T29/49—Method of mechanical manufacture
- Y10T29/49002—Electrical device making
- Y10T29/49117—Conductor or circuit manufacturing
Abstract
The device is a multi-cell test device, comprising:
a plurality of coplanar electrochemical cells, comprising, in sequence:
(a) a common first substrate (1101) having a layer of conductive material applied to a first surface thereof;
(b) a common electrically-resistive middle layer (1104); and
(c) a common second substrate (1102) having a layer of conductive material applied to a first surface thereof, the first surface of the first substrate and the first surface of the second substrate being adhered to the electrically resistive middle layer. Each electrochemical cell comprises:
a sample-receiving end,
a connector end having a plurality of connectors, and
a sample space passing through the electrically resistive middle layer, bounded on opposing sides by a portion of the conductive material of the first and second substrates forming an unpatterned first electrode, and being connected with the sample-receiving end of the device.
Description
Description Electrochemical Assay Device and Related Methods [1] This application claims the benefit of US Provisional Application No.
60/521,555 filed May 21, 2004, which application is incorporated herein by reference.
Background of the Invention [2] This application relates to electrochemical assay devices in the form of single use test strips for detecting the presence or amount of an analyte in a sample and to methods of making and using such devices.
60/521,555 filed May 21, 2004, which application is incorporated herein by reference.
Background of the Invention [2] This application relates to electrochemical assay devices in the form of single use test strips for detecting the presence or amount of an analyte in a sample and to methods of making and using such devices.
[3] Single use disposable test strips for the electrochemical detection of analytes such as glucose are known. In such test strips, a sample is introduced into the test strip to contact at least two electrodes. Oxidation or reduction of the analyte is observed as a current generated between the two electrodes. Using glucose detection in a conduction cell as an example, as illustrated in Fig. 1, glucose is oxidized by the enzyme glucose oxidase to form gluconolactone and reduced enzyme. The oxidized form of the enzyme is regenerated by reaction with an oxidized mediator with the resulting generation of reduced mediator. This reduced mediator transfers an electron to one electrode, while at the other electrode electrons are transferred onto oxidized mediator, thus producing an observable current. Fig. 2 shows the observable current as a function of time in a test strip using an enzyme/mediator reagent system. In the figure, t=0 is the time of sample application. As shown, the current rises through a maximum, and then declines to reach an eventual steady state plateau. Measurements to determine the amount of analyte are taken after the maximum current has been reached, and generally at a time after the steady state has been achieved.
[4] Before the maximum is reached in Fig. 2, there is a delay observed which can constitute a significant portion of the overall measurement time. The duration of this delay is dependent on the distance between the electrodes, and on the mobility of the mediator employed in the test strip. Mediator mobility is a property of the mediator itself, i.e., the diffusion coefficient, but is also dependent on other sample properties such as hematocrit and viscosity.
[5] In order to increase user convenience, improvements to analyte test strips generally, and to glucose test strips in particular have focused on two major goals:
shorter test times and smaller sample volumes. To some extent, these two goals have been achieved in tandem, since smaller sample volumes use smaller cells with smaller electrode-spacing, and smaller electrode-spacing results in shorter reaction time. These cells still have the current/time profile of Fig. 2, however, and thus built-in delay before a measurement can be taken. The present invention eliminates this delay, and thus provides a significant reduction in the time required to complete a test.
Summary of the Invention [6] In accordance with the present invention, an electrochemical test device is provided having a base layer with a first electrode thereon and a top layer with a second electrode thereon. The two electrodes are separated by a spacer layer having an opening therein, such that a sample-receiving space is defined with one electrode on the top surface, the other electrodes on the bottom surface and side walls formed from edges of the opening in the spacer.
shorter test times and smaller sample volumes. To some extent, these two goals have been achieved in tandem, since smaller sample volumes use smaller cells with smaller electrode-spacing, and smaller electrode-spacing results in shorter reaction time. These cells still have the current/time profile of Fig. 2, however, and thus built-in delay before a measurement can be taken. The present invention eliminates this delay, and thus provides a significant reduction in the time required to complete a test.
Summary of the Invention [6] In accordance with the present invention, an electrochemical test device is provided having a base layer with a first electrode thereon and a top layer with a second electrode thereon. The two electrodes are separated by a spacer layer having an opening therein, such that a sample-receiving space is defined with one electrode on the top surface, the other electrodes on the bottom surface and side walls formed from edges of the opening in the spacer.
[7] In a conduction cell where the reagents for performing the electrochemical reaction are deposited on one of the electrodes but not the side walls, the device produces a signal profile as in Fig. 2. In the device of the invention, the reagents are deposited not only on this electrode, but also on the side walls of the sample- receiving space. (Fig.
3) This results in a signal profile without the lag or with a reduced lag.
(Fig. 4). In preferred embodiments, the reagents extend over the entire height of the side walls, although significant improvements are achievable with as little as 25% of the height of the side wall being coated with reagent.
3) This results in a signal profile without the lag or with a reduced lag.
(Fig. 4). In preferred embodiments, the reagents extend over the entire height of the side walls, although significant improvements are achievable with as little as 25% of the height of the side wall being coated with reagent.
[8] The invention also provides a method of making test strips of the invention. In accordance with this method, an interniediate structure is formed comprising a base layer and a spacer layer disposed thereon. The base layer has the first electrode disposed thereon, and this electrode is exposed through an opening in the spacer layer.
Thus, a well or channel is defined by the first electrode/base layer and the edges of the opening spacer layer. A liquid containing the reagents is introduced to the well or channel in such a way that it at least partially, and preferably completely, covers the side walls of the well. The liquid is then dried, leaving a coating of reagents on the bottom (first electrode) and side walls of the well/channel. Thereafter a top layer and a second electrode are added over the well/channel.
Thus, a well or channel is defined by the first electrode/base layer and the edges of the opening spacer layer. A liquid containing the reagents is introduced to the well or channel in such a way that it at least partially, and preferably completely, covers the side walls of the well. The liquid is then dried, leaving a coating of reagents on the bottom (first electrode) and side walls of the well/channel. Thereafter a top layer and a second electrode are added over the well/channel.
[9] In a preferred embodiment of the method, the spacer layer has an adhesive coating and a release sheet on the side opposite the base layer and the side walls of the well extend upwards through the release sheet. Reagent material is introduced to the well/
channel such that at least some part of the release sheet side wall is covered with the reagent-containing liquid prior to drying and preferably with reagent after drying.
Subsequent removal of the release sheet results in a well in which the side walls are substantially completely covered with dried reagent.
Brief Description of the Drawings [10] Fig. 1 shows the basic chemical reactions employed in a glucose test strip.
channel such that at least some part of the release sheet side wall is covered with the reagent-containing liquid prior to drying and preferably with reagent after drying.
Subsequent removal of the release sheet results in a well in which the side walls are substantially completely covered with dried reagent.
Brief Description of the Drawings [10] Fig. 1 shows the basic chemical reactions employed in a glucose test strip.
[11] Fig 2 shows current as a function of time in a conductance cell test strip with the reagents applied only to the bottom of the well.
[12] Fig. 3 shows a cross section through the sample-receiving space of a device in accordance with the invention.
[13] Fig. 4 shows current as a function of time in a test strip in accordance with the invention.
[14] Fig. 5 shows a cross section through the sample-receiving space of a device in accordance with the invention.
[15] Fig. 6 shows a diagrammatic representation of the method of the invention.
[16] Fig. 7A and B show diagrammatic representations of the method of the invention.
Detailed Description of the Invention [17] The application relates to electrochemical test devices or strips, of the type commonly used in the assay of blood glucose.
Definitions [18] As used in the specification and claims of this application, the term "elec-trochemical test device" refers to a device which provides, alone or in combination with a reusable meter, a determination of an analyte in a sample using an elec-trochemical assay. Preferred electrochemical test devices are disposable single use devices of the type generally known for home determination of glucose levels.
Detailed Description of the Invention [17] The application relates to electrochemical test devices or strips, of the type commonly used in the assay of blood glucose.
Definitions [18] As used in the specification and claims of this application, the term "elec-trochemical test device" refers to a device which provides, alone or in combination with a reusable meter, a determination of an analyte in a sample using an elec-trochemical assay. Preferred electrochemical test devices are disposable single use devices of the type generally known for home determination of glucose levels.
[19] The term "analyte" as used in the specification and claims of this application means a component of a sample to be measured. Non-limiting examples of specific analytes include glucose, hemoglobin, cholesterol, and vitamin C.
[20] As used in the specification and claims of this application the term "electrode"
refers to a component of the device which transfers electrons to or from species in a sample introduced into the sample-receiving space of the electrochemical test device, and which is or can be connected to circuitry to determine the amount of electron transfer occurring, either as a current flow or a potential difference between electrodes contacting the same sample. The electrodes in the devices of the invention are made from conductive materials consistent with the specific analyte that the electrochemical cell is intended to detect. Specific examples of suitable conductive electrode materials include gold, carbon, silver, palladium, and platinum. The conductive material used in the first and second electrodes may be the same or they may be different from one another. In a preferred embodiment of the present invention the conductive material used to form the electrodes is gold.
refers to a component of the device which transfers electrons to or from species in a sample introduced into the sample-receiving space of the electrochemical test device, and which is or can be connected to circuitry to determine the amount of electron transfer occurring, either as a current flow or a potential difference between electrodes contacting the same sample. The electrodes in the devices of the invention are made from conductive materials consistent with the specific analyte that the electrochemical cell is intended to detect. Specific examples of suitable conductive electrode materials include gold, carbon, silver, palladium, and platinum. The conductive material used in the first and second electrodes may be the same or they may be different from one another. In a preferred embodiment of the present invention the conductive material used to form the electrodes is gold.
[21] As used in the specification and claims of this application, the term "spacer" refers to a layer of material providing electrical separation between the two electrodes of the device. Thus, the spacer is a generally insulating material and electrical contact between the electrodes occurs only in the presence of a sample in the sample-receiving space. In preferred embodiments, the spacer is formed from a film or sheet of an insulating material. Examples of suitable materials include, without limitation, polyimide, polyester, polyethylene terephthalate (PET), polycarbonate, glass, and fiberglass. The spacer may also be formed by deposition of an insulating layer, for example by spraying on a resistive coating. Openings can be formed in such layers using conventional techniques including pre-cutting of an opening in a defined film or sheet, laser or chemical etching and the like.
[22] As used in the specification and claims of this application, the term "reagent" refers to a chemical or mixture of chemicals that when combined with a sample allows the electrochemical test device to be used in making a determination of analyte in the sample. The reagent need not be sufficient to allow this determination, and addition of further chemicals to the sample prior to the introduction to the test device is acceptable, although not preferred. The reagent does, however, at a minimum contain a redox active material which is oxidized at the first electrode and reduced at the second electrode (or vice versa) when the device is used. The reagent may include multiple redox active materials which act as charge carrier between the electrodes.
[23] As used in the specification and claims of this application, the phrase "de-termination of analyte" refers to and encompasses qualitative detection of the presence, of the analyte, that is whether or not the analyte is present in detectable amounts in the sample, semiquantitative detection, that is whether or not the analyte is present in an .
amount greater than a predetermined threshold value, and quantitative evaluation, that is determination of the actual numerical amount of the analyte that is present.
amount greater than a predetermined threshold value, and quantitative evaluation, that is determination of the actual numerical amount of the analyte that is present.
[24] As used in the specification and claims of this application, the terms "cover" or "covering" refers to coating of the specified surface. It does not require complete covering, for example in the case where pores may exist in the coating, but merely a distribution of the covering reagent over the specified surface. Further, it does not exclude coatings that may be less than complete due to unintentional flaws in the coating process in a specific device.
[25] As used in the specification and claims of this application, the phrase "portion of the side walls extending contiguously from the covered electrode" refers to a coating where the reagent coating over the electrode flows into the reagent coating on the side walls.
Device of the Invention [26] Fig. 3 shows a cross section through the sample-receiving space of a device in accordance with the invention. As shown, a substrate layer 31 has an electrode disposed thereon. Spacer layer 33 has an opening therein which provides side walls 34, 34'. Top substrate layer 35 has an electrode 36 disposed thereon. Sample receiving space 37 is bounded by electrodes 32 and 36 and side walls 34 and 34' and contains dried reagent 38. In an alternative embodiment, the sample receiving space may have top and bottom surfaces that are partially covered by the electrodes and partially exposed top layer. The dried reagent 38 covers the electrode 32 at the bottom of the sample receiving space 37 and extends upwards along the side walls 34, 34'.
Device of the Invention [26] Fig. 3 shows a cross section through the sample-receiving space of a device in accordance with the invention. As shown, a substrate layer 31 has an electrode disposed thereon. Spacer layer 33 has an opening therein which provides side walls 34, 34'. Top substrate layer 35 has an electrode 36 disposed thereon. Sample receiving space 37 is bounded by electrodes 32 and 36 and side walls 34 and 34' and contains dried reagent 38. In an alternative embodiment, the sample receiving space may have top and bottom surfaces that are partially covered by the electrodes and partially exposed top layer. The dried reagent 38 covers the electrode 32 at the bottom of the sample receiving space 37 and extends upwards along the side walls 34, 34'.
[27] Fig. 4 shows a current/time profile for a device in accordance with the invention in which the dried reagent covers substantially all of the side walls. A
comparison of this figure with Fig. 2 shows the clear advantage of the invention, namely that the current starts immediately, and steady state is achieved in less time.
comparison of this figure with Fig. 2 shows the clear advantage of the invention, namely that the current starts immediately, and steady state is achieved in less time.
[28] While not intending to be bound to any specific mechanism, it is believed that this effect occurs because charge carriers are present in proximity to both electrodes from the outset, and therefore current can be generated immediately. In contrast, when the reagent is applied solely on the first electrode surface, the chemical reactions may conunence immediately on sample addition but substantial current cannot flow until mediator (or some other redox active species) diffuses from the first electrode to the second. This takes time, and therefore there is a delay before an analyte-dependent current is observed. In addition, in the case of a reagent with a small amount of active mediator, the reaction cannot even commence beyond a certain point until a counter reaction is available. This delay in the onset of the chemical reaction is cumulative with delays due to other diffusion processes.
[29] Based on this mechanism, theory predicts that the delay in time will be related to the square of the distance the charge carrier must travel to reach the second electrode.
This means that if the distance between the dried reagent and the second electrode is cut in half, the time will be reduced by a factor of 4, and that even coating 25% of the side wall will result in a reduction in the time required to reach the current maximum by a factor of about 2. Thus, in the devices of the invention, at least 25%, preferably at least 50%, more preferably at least 75%, and most preferably all of the side wall extending above the first electrode is coated with dried reagent.
This means that if the distance between the dried reagent and the second electrode is cut in half, the time will be reduced by a factor of 4, and that even coating 25% of the side wall will result in a reduction in the time required to reach the current maximum by a factor of about 2. Thus, in the devices of the invention, at least 25%, preferably at least 50%, more preferably at least 75%, and most preferably all of the side wall extending above the first electrode is coated with dried reagent.
[30] This mechanism also makes it clear that the important component in the reagent is the mediator or charge carrier. Thus, as illustrated in Fig. 5, in an alternative embodiment of the invention, a reagent layer 51 comprising an enzyme such as glucose oxidase, is deposited on the surface of the first electrode, and a redox active coating 52 is deposited to cover the bottom and at least a portion of the sides of the sample-receiving space.
[31] The redox active coating 52, or the reagent coating 38 may contain both redox states of the redox active species used in the device. This may be the reduced form, the oxidized form, or a mixture thereof. Specific non-limiting examples of redox active species are redox mediators known for use in glucose and other mediated elec-trochemical detection systems. The term "redox mediator" as used in the specification and claims of this application means a chemical species, other than the analyte, that is oxidized and/or reduced in the course of a multi-step process transferring electrons to or from the analyte to an electrode of the electrochemical cell. Non-limiting examples of mediators include:
= ferricyanide = [FeIII(CN)5(ImH)]2-= [FeIII(CN)5(Im)]3-= [RuIII(NH3)5(ImH)]3+
= [RuIII(NH3)5(Im)]2+
= [FeII(CN)5(ImH)]3-= [RuII(NH3)5(Im)H]2+
= [(NC)5FeII(Im)RuIII(NH3)5]-= [(NC)5FeIII(Im)RuIII(NH3)5]
= [(NC)5FeII(Im)RuII(NH3)5]2-= Ferrocene (Fc) and derivatives including but not limited to:
= Ferrocene monosulphonate = Ferrocene disulphonate = FcCO H
= FcCH2CO H
= FcCH:CHCO H
= Fc(CH2)3CO2H
= Fc(CH2)4CO,,H
= FcCH2CH(NH2)CO2H
= FcCH 2 SCH 2 CH(NH 2 )CO2H
= FcCH CONH
= Fc(CH ) CONH
= Fc(CH ) CONH
= Fc(CH2)4CONH2 = FcOH
= FcCH OH
= Fc(CH2)2OH
= FcCH(Me)OH
= FcCH2O(CH) 2OH
= 1,1'-Fc(CH2OH)2 = 1,2-Fc(CH2OH)2 = FcNH
= FcCH NH
9 Fc(CH2)2NH2 = Fc(CH2)3NH2 = l,l'-Me2FcCH2NH2 = FcCH NMe = (R)-FcCH(Me)NMe2 = (S)-FcCH(Me)NMe2 = 1,2-Me SiFcCH NMe = FcCH NMe = FcCH NH(CH ) NH
= 1,1'-Me FcCH(OH)CH NH
= FcCH(OH)CH2NH2 = FcCH:CHCH(OH)CH2NH2 = Fc(CH ) CH(OH)CH NH
= FcCH2CH(NH2)CH2OH
= FcCH 2 CH(CH 2 NH 2 )CH 2 OH
= FcCH2NH(CH,,)2OH
= 1,1'-MeFcCHOCONHCH~
= FcCH(OH)(CH2)2NH2 = 1,1'-Me2FcCH(OH)CH2NHAc = FcB(OH) 3 = FcC H OPO Na = Osmium II and Osmium III tris(phenanthroline) (i.e. Os-phen) complexes including but not limited to:
= Os(4,7-dmphen) 3 = Os(3,4,7,8-tmphen) 3 = Os(5,6-dmphen) 3 = Os(bpy)3Cl2 = Os(5-mphen) = Os(5-Cl-phen) 3 = Os(5-NO2 -phen)3 = Os(5-phphen) 3 = Os(2,9-dm-4,7-dpphen) 3 = and isostructural ruthenium complexes including but not limited to:
= Ru(4,7-dmphen) 3 = Ru(3,4,7,8-tmphen) 3 = Ru(5-mphen) 3 = Ru(5,6-dmphen) 3 = Ru(phen) 3 0 [Ru(4,4'-diNH2 bipy)3]2+
= Osmium II and Osmium III tris(bipyridyl) complexes (i.e. Os(bpy)3) including but not limited to:
' Os(bpy)3 = Os(dmbpy) 3 = and related ruthenium complexes, e.g.:
' Ru(bpy) 3 = Ru(4,4'-diNH2 bpy)3 = Ru(4,4'-diCO2 Etbpy)3 = Osmium II and Osmium III bis(bipyridyl) (i.e. Os(bpy)Z) complexes with other ligands including but not limited to:
= Os(bpy)2dmbpy = Os(bpy)2(HIm), = Os(bpy)Z(2MeHIm), = Os(bpy)2(4MeHIm)2 = Os(dmbpy)~(HIm)2 = Os(bpy)2C1(HIm) = Os(bpy)2C1(1-Melm) = Os(dmbpy)2C1(HIm) = Os(dmbpy)2C1(1-Melm) = and related ruthenium complexes, e.g.:
= Ru(bpy) 2 (5,5'diNH2-bpy) = Ru(bpy),(5,5' diCO2Etbpy) = Ru(bpy)2(4,4' diCOyEtbpy) [32] where Et is ethyl, bpy is bipyridyl, dmbpy is dimethyl bipyridyl, Melm is N-methyl imidazole, MeHIm is methyl imidazole, HIm is imidazole, phen is phenanthroline, mphen ismethyl phenantholine, dmphen is dimethyl phenanthroline, tmphen is tetramethyl phenanthroline, dmdpphen is dimethyl diphenyl phenanthroline, phphen is phenyl phenanthroline. In addition, it is understood that reduced or oxidized forms of these mediators may be used, either alone or in combination with each other.
Method of the Invention [33] The present invention also provides a method of making an electrochemical test device of the type described above. This method is illustrated schematically in Fig 6.
As shown, a spacer layer 61 is disposed on a first electrode 62. This can be readily ac-complished using an insulating film or sheet that is coated with adhesive on both sides of the spacer layer 61. The spacer layer 61 has an opening 63 through which the first electrode 62 is exposed. This opening can be in the form of a well 63 as depicted in Fig. 6, or as a channel 73 as shown in Fig. 7A and B. An insulating support 64 is under the electrode 62.
= ferricyanide = [FeIII(CN)5(ImH)]2-= [FeIII(CN)5(Im)]3-= [RuIII(NH3)5(ImH)]3+
= [RuIII(NH3)5(Im)]2+
= [FeII(CN)5(ImH)]3-= [RuII(NH3)5(Im)H]2+
= [(NC)5FeII(Im)RuIII(NH3)5]-= [(NC)5FeIII(Im)RuIII(NH3)5]
= [(NC)5FeII(Im)RuII(NH3)5]2-= Ferrocene (Fc) and derivatives including but not limited to:
= Ferrocene monosulphonate = Ferrocene disulphonate = FcCO H
= FcCH2CO H
= FcCH:CHCO H
= Fc(CH2)3CO2H
= Fc(CH2)4CO,,H
= FcCH2CH(NH2)CO2H
= FcCH 2 SCH 2 CH(NH 2 )CO2H
= FcCH CONH
= Fc(CH ) CONH
= Fc(CH ) CONH
= Fc(CH2)4CONH2 = FcOH
= FcCH OH
= Fc(CH2)2OH
= FcCH(Me)OH
= FcCH2O(CH) 2OH
= 1,1'-Fc(CH2OH)2 = 1,2-Fc(CH2OH)2 = FcNH
= FcCH NH
9 Fc(CH2)2NH2 = Fc(CH2)3NH2 = l,l'-Me2FcCH2NH2 = FcCH NMe = (R)-FcCH(Me)NMe2 = (S)-FcCH(Me)NMe2 = 1,2-Me SiFcCH NMe = FcCH NMe = FcCH NH(CH ) NH
= 1,1'-Me FcCH(OH)CH NH
= FcCH(OH)CH2NH2 = FcCH:CHCH(OH)CH2NH2 = Fc(CH ) CH(OH)CH NH
= FcCH2CH(NH2)CH2OH
= FcCH 2 CH(CH 2 NH 2 )CH 2 OH
= FcCH2NH(CH,,)2OH
= 1,1'-MeFcCHOCONHCH~
= FcCH(OH)(CH2)2NH2 = 1,1'-Me2FcCH(OH)CH2NHAc = FcB(OH) 3 = FcC H OPO Na = Osmium II and Osmium III tris(phenanthroline) (i.e. Os-phen) complexes including but not limited to:
= Os(4,7-dmphen) 3 = Os(3,4,7,8-tmphen) 3 = Os(5,6-dmphen) 3 = Os(bpy)3Cl2 = Os(5-mphen) = Os(5-Cl-phen) 3 = Os(5-NO2 -phen)3 = Os(5-phphen) 3 = Os(2,9-dm-4,7-dpphen) 3 = and isostructural ruthenium complexes including but not limited to:
= Ru(4,7-dmphen) 3 = Ru(3,4,7,8-tmphen) 3 = Ru(5-mphen) 3 = Ru(5,6-dmphen) 3 = Ru(phen) 3 0 [Ru(4,4'-diNH2 bipy)3]2+
= Osmium II and Osmium III tris(bipyridyl) complexes (i.e. Os(bpy)3) including but not limited to:
' Os(bpy)3 = Os(dmbpy) 3 = and related ruthenium complexes, e.g.:
' Ru(bpy) 3 = Ru(4,4'-diNH2 bpy)3 = Ru(4,4'-diCO2 Etbpy)3 = Osmium II and Osmium III bis(bipyridyl) (i.e. Os(bpy)Z) complexes with other ligands including but not limited to:
= Os(bpy)2dmbpy = Os(bpy)2(HIm), = Os(bpy)Z(2MeHIm), = Os(bpy)2(4MeHIm)2 = Os(dmbpy)~(HIm)2 = Os(bpy)2C1(HIm) = Os(bpy)2C1(1-Melm) = Os(dmbpy)2C1(HIm) = Os(dmbpy)2C1(1-Melm) = and related ruthenium complexes, e.g.:
= Ru(bpy) 2 (5,5'diNH2-bpy) = Ru(bpy),(5,5' diCO2Etbpy) = Ru(bpy)2(4,4' diCOyEtbpy) [32] where Et is ethyl, bpy is bipyridyl, dmbpy is dimethyl bipyridyl, Melm is N-methyl imidazole, MeHIm is methyl imidazole, HIm is imidazole, phen is phenanthroline, mphen ismethyl phenantholine, dmphen is dimethyl phenanthroline, tmphen is tetramethyl phenanthroline, dmdpphen is dimethyl diphenyl phenanthroline, phphen is phenyl phenanthroline. In addition, it is understood that reduced or oxidized forms of these mediators may be used, either alone or in combination with each other.
Method of the Invention [33] The present invention also provides a method of making an electrochemical test device of the type described above. This method is illustrated schematically in Fig 6.
As shown, a spacer layer 61 is disposed on a first electrode 62. This can be readily ac-complished using an insulating film or sheet that is coated with adhesive on both sides of the spacer layer 61. The spacer layer 61 has an opening 63 through which the first electrode 62 is exposed. This opening can be in the form of a well 63 as depicted in Fig. 6, or as a channel 73 as shown in Fig. 7A and B. An insulating support 64 is under the electrode 62.
[34] A liquid reagent 65 comprising a redox active material is introduced into the opening 63/73 in the spacer layer 61 in such a way as to cover at least a portion of, but preferably all of, the exposed first electrode 62 and at least a portion of the side walls 66 of the opening 63/73. In one embodiment of the invention, this result is achieved by filling the opening 63/73 to a sufficient depth to at least partially cover the side walls.
The result may also be achieved by applying the reagent along the electrode and side walls as a moving droplet on a dispensing tip so that it leaves a wetted trail, or using a ink jet or similar dispenser on a trajectory that results in wetted side walls as well as a wetted electrode. It should be noted that at the small scales generally employed in devices for glucose testing, the surface tension pulls the reagent to cover all wettted surfaces such that reagent surface area is minimized. This helps reagent spread into corners and up walls if already wetted.
The result may also be achieved by applying the reagent along the electrode and side walls as a moving droplet on a dispensing tip so that it leaves a wetted trail, or using a ink jet or similar dispenser on a trajectory that results in wetted side walls as well as a wetted electrode. It should be noted that at the small scales generally employed in devices for glucose testing, the surface tension pulls the reagent to cover all wettted surfaces such that reagent surface area is minimized. This helps reagent spread into corners and up walls if already wetted.
[35] The liquid reagent 65 is then dried in the opening 63/73 to form a dried reagent disposed in a layer covering the first electrode 62 and at least a portion of the side walls 66. The drying may be accomplished by simply leaving the structure to dry in air, drying in an applied air stream, heating, drying in a heated air stream, drying under vacuum, or drying in a heated vacuum. A sheet of materia167 with a conductive .
electrode surface 68 is then applied over the top of the spacer sheet, to form a second electrode facing the first electrode 62 across the opening 63. A sample introduction opening to the sample receiving space can be created by trimming the intermediate, structure thus formed transversely through the opening 63/73 and contacts are formed with the electrodes to allow connection to an external meter. A preferred method for forming a device is described in US Provisional Patent No. 60/521,555 and US
Patent Application Serial No. 10/908,656 filed May 20, 2005, which is incorporated herein by reference.
Example [36] Two devices were constructed using facing gold electrodes and a reagent comprising glucose oxidase, ferricyanide, buffer salts, and soluble stabilizers. In the device in accordance with the invention, the reagent extended up the side walls into proximity with the second electrode. In the control device, the reagent was disposed only on the first electrode. A 300 mV potential was applied (positive electrode was the first electrode with reagent; negative electrode was the second electrode without reagent) to each device as soon as the sample entered the strip (t=0). As shown in Fig.
2, in the control device the initial few seconds have no real current, and the current only increases beginning at about 1 sec. The current cannot increase significantly until the reagent can dissolve and diffuse to the second electrode, thereby providing a counter reaction to allow current to flow. Fig 4 shows the current profile for the device in accordance with the invention. In this case, the reagent dissolves and diffuses almost as soon as the sample enters the strip and current begins to flow virtually immediately (t=O). Thus, a steady-state current is achieved in the sandwich geometry electrode structure of this device at about 3 seconds, as opposed to about 5 seconds for the otlier device.
electrode surface 68 is then applied over the top of the spacer sheet, to form a second electrode facing the first electrode 62 across the opening 63. A sample introduction opening to the sample receiving space can be created by trimming the intermediate, structure thus formed transversely through the opening 63/73 and contacts are formed with the electrodes to allow connection to an external meter. A preferred method for forming a device is described in US Provisional Patent No. 60/521,555 and US
Patent Application Serial No. 10/908,656 filed May 20, 2005, which is incorporated herein by reference.
Example [36] Two devices were constructed using facing gold electrodes and a reagent comprising glucose oxidase, ferricyanide, buffer salts, and soluble stabilizers. In the device in accordance with the invention, the reagent extended up the side walls into proximity with the second electrode. In the control device, the reagent was disposed only on the first electrode. A 300 mV potential was applied (positive electrode was the first electrode with reagent; negative electrode was the second electrode without reagent) to each device as soon as the sample entered the strip (t=0). As shown in Fig.
2, in the control device the initial few seconds have no real current, and the current only increases beginning at about 1 sec. The current cannot increase significantly until the reagent can dissolve and diffuse to the second electrode, thereby providing a counter reaction to allow current to flow. Fig 4 shows the current profile for the device in accordance with the invention. In this case, the reagent dissolves and diffuses almost as soon as the sample enters the strip and current begins to flow virtually immediately (t=O). Thus, a steady-state current is achieved in the sandwich geometry electrode structure of this device at about 3 seconds, as opposed to about 5 seconds for the otlier device.
Claims (17)
- [1] 1. An electrochemical test device comprising a bottom substrate having a first electrode disposed thereon, a top substrate having a second electrode disposed thereon, and a spacer disposed between the top and bottom substrates and having an opening therein, whereby a sample-receiving space is defined that has a bottom surface having the first electrode disposed thereon, a top surface, opposite the first surface, having the second electrode disposed thereon, and side walls formed from edges of the opening in the spacer; and a reagent comprising a redox active material which is oxidized at the first electrode and reduced at the second electrode when the device is used;
wherein in the test device prior to introduction of a liquid sample, the reagent is disposed in a layer covering at least a portion of the first or second electrode and at least a portion of the side walls. - [2] 2. The device of claim 1, wherein the reagent covers at least 25% of the height of the side walls.
- [3] 3. The device of claim 1, wherein the reagent covers at least 50% of the height of the side walls.
- [4] 4. The device of claim 1, wherein the reagent covers at least 75% of the height of the side walls.
- [5] 5. The device of claim 1, wherein the reagent covers 100% of the height of the side walls.
- [6] 6. The device of any of claims 1 to 5, wherein the redox active material is selected from the group consisting of ferricyanide [FeIII(CN)5(ImH)]2-[FeIII(CN)5(Im)]3-[RuIII(NH3)5(ImH)]3+
[RuIII(NH3)5(Im)]2+
[FeII(CN)5(ImH)]3-[RuII(NH3)5(Im)H]2+
[(NC)5FeII(Im)RuIII(NH3)5]-[(NC)5FeIII(Im)RuIII(NH3)5]0 [(NC)5FeII(Im)RuII(NH3)5]2-Ferrocene (Fc) Ferrocene monosulphonate Ferrocene disulphonate FcCO2H
FcCH2CO2H
FcCH:CHCO2H
Fc(CH2)3CO2H
Fc(CH2)4CO2H
FcCH2CH(NH2)CO2H
FcCH2SCH2CH(NH2)CO2H
FcCH2CONH
Fc(CH2)2CONH2 Fc(CH2)3CONH2 Fc(CH2)4 CONH2 FcOH
FcCH2OH
Fc(CH2)2OH
FcCH(Me)OH
FcCH2O(CH2)2OH
1,1'-Fc(CH2OH)2 1,2-Fc(CH2OH)2 FcNH2 FcCH2NH2 Fc(CH2)2NH2 Fc(CH2)3NH2 1,1'-Me2FcCH2NH2 FcCH2NMe2 (R)-FcCH(Me)NMe2 (S)-FcCH(Me)NMe2 1,2-Me3SiFcCH2NMe2 FcCH2NMe3 FcCH2NH(CH2)2NH2 1,1'-Me2 FcCH(OH)CH2NH2 FcCH(OH)CH2NH2 FcCH:CHCH(OH)CH2NH2 Fc(CH2)2CH(OH)CH2NH2 FcCH2CH(NH2)CH2OH
FcCH2CH(CH2NH2)CH2OH
FcCH2NH(CH2)2OH
1,1'-Me2FcCHOCONHCH2 FcCH(OH)(CH2)2NH2 1,1'-Me2FcCH(OH)CH2NHAc FcB(OH)3 FcC6H4OPO3Na2 Os(4,7-dmphen)3 Os(3,4,7,8-tmphen)3 Os(5,6-dmphen)3 Os(bpy)3Cl2 Os(5-mphen)3 Os(5-Cl-phen)3 Os(5-NO2-phen)3 Os(5-phphen)3 Os(2,9-dm-4,7-dpphen)3 Ru(4,7-dmphen)3 Ru(3,4,7,8-tmphen)3 Ru(5-mphen)3 Ru(5,6-dmphen)3 Ru(phen)3 [Ru(4,4'-diNH2 -bipy)3]2+
Os(bpy)3 Os(dmbpy)3 Ru(bpy)3 Ru(4,4'-diNH2-bpy)3 Ru(4,4' -diCO2Etbpy)3 Os(bpy)2dmbpy Os(bpy)2(HIm)2 Os(bpy)2(2MeHIm)2 Os(bpy)2(4MeHIm)2 Os(dmbpy)2(HIm)2 Os(bpy)2Cl(HIm) Os(bpy)2Cl(1-MeIm) Os(dmbpy)2Cl(HIm) Os(dmbpy)2Cl(1-MeIm) Ru(bpy)2(5,5'diNH2-bpy) Ru(bpy)2(5,5' diCO2Etbpy) Ru(bpy)2(4,4' diCO2Etbpy) or the complementary redox forms (oxidized or reduced) thereof. - [7] 7. The device of any of claims 1 to 6, wherein the reagent further comprises glucose oxidase.
- [8] 8. A method of making an electrochemical test device comprising the steps of:
(a) forming a bottom substrate having a first electrode disposed thereon;
(b) forming a spacer layer on the bottom substrate, said spacer layer having an opening formed therein through which the first electrode is exposed and side walls within the opening;
(c) introducing a liquid reagent comprising a redox active material into the opening in the spacer layer, (d) drying the liquid reagent to form a dried reagent, wherein the liquid reagent is introduced to the opening in such a manner that upon drying a dried reagent layer is formed covering at least a portion of the first electrode and at least a portion of the side walls ; and (e) placing a top substrate having a second electrode disposed thereon on the spacer layer aligned to have the second electrode facing the first electrode, thereby forming a sample-receiving space that has a first surface having the first electrode disposed thereon, a second surface, opposite the first surface, having the second electrode disposed thereon, and side walls formed from edges of the opening in the spacer. - [9] 9. The method of claim 8, wherein the dried reagent covers at least 25% of the height of the side walls.
- [10] 10. The method of claim 8 wherein the dried reagent covers at least 50%
of the height of the side walls. - [11] 11. The method of claim 8, wherein the dried reagent covers at least 75%
of the height of the side walls. - [12] 12. The method of claim 8, wherein the reagent covers 100% of the height of the side walls.
- [13] 13. The method of any of claims 8 to 12, wherein the spacer layer applied in step (b) comprises an adhesive coating and a release sheet disposed on the side of the spacer layer facing away from the first electrode, whereby a portion of the side walls is formed from the release sheet, further comprising the step of removing the release sheet after drying of the liquid reagent to expose the adhesive layer.
- [14] 14. The method of claim 13, wherein the liquid reagent is introduced to the opening in a volume sufficient to fill the opening to a level that at least partially covers the portion of the side walls formed from the release sheet.
- [15] 15. The method of any of claims 8 to 12, wherein the liquid reagent is introduced in a volume sufficient to fill the opening to a level that partially covers the portion of the side walls of the opening.
- [16] 16. The method of any of claims 8 to 15, wherein the opening is in the form of a well that is bounded by side walls on all sides.
- [17] 17. The method of any of claims 8 to 15, wherein the opening is in the form of a channel bounded by side walls on only two opposing sides.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US52155504P | 2004-05-21 | 2004-05-21 | |
US60/521,555 | 2004-05-21 | ||
PCT/IB2005/051659 WO2005113790A1 (en) | 2004-05-21 | 2005-05-21 | Electrochemical assay device and related methods |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2565523A1 true CA2565523A1 (en) | 2005-12-01 |
Family
ID=34978606
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2566358A Active CA2566358C (en) | 2004-05-21 | 2005-05-20 | Electrochemical cell and method for making an electrochemical cell |
CA002566214A Abandoned CA2566214A1 (en) | 2004-05-21 | 2005-05-21 | Connector configuration for electrochemical cells and meters for use in combination therewith |
CA002565523A Abandoned CA2565523A1 (en) | 2004-05-21 | 2005-05-21 | Electrochemical assay device and related methods |
Family Applications Before (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2566358A Active CA2566358C (en) | 2004-05-21 | 2005-05-20 | Electrochemical cell and method for making an electrochemical cell |
CA002566214A Abandoned CA2566214A1 (en) | 2004-05-21 | 2005-05-21 | Connector configuration for electrochemical cells and meters for use in combination therewith |
Country Status (14)
Country | Link |
---|---|
US (8) | US8268145B2 (en) |
EP (4) | EP1756557B1 (en) |
JP (3) | JP5215661B2 (en) |
KR (5) | KR101328608B1 (en) |
CN (6) | CN103353475B (en) |
AT (1) | ATE480636T1 (en) |
AU (6) | AU2005246102B8 (en) |
CA (3) | CA2566358C (en) |
DE (1) | DE602005023435D1 (en) |
ES (2) | ES2569059T3 (en) |
HK (3) | HK1178598A1 (en) |
NO (3) | NO20065935L (en) |
PT (1) | PT1747281E (en) |
WO (3) | WO2005114159A1 (en) |
Families Citing this family (111)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6036924A (en) | 1997-12-04 | 2000-03-14 | Hewlett-Packard Company | Cassette of lancet cartridges for sampling blood |
US6391005B1 (en) | 1998-03-30 | 2002-05-21 | Agilent Technologies, Inc. | Apparatus and method for penetration with shaft having a sensor for sensing penetration depth |
US20060091006A1 (en) * | 1999-11-04 | 2006-05-04 | Yi Wang | Analyte sensor with insertion monitor, and methods |
US6616819B1 (en) * | 1999-11-04 | 2003-09-09 | Therasense, Inc. | Small volume in vitro analyte sensor and methods |
US8641644B2 (en) | 2000-11-21 | 2014-02-04 | Sanofi-Aventis Deutschland Gmbh | Blood testing apparatus having a rotatable cartridge with multiple lancing elements and testing means |
US8337419B2 (en) | 2002-04-19 | 2012-12-25 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
EP1404234B1 (en) | 2001-06-12 | 2011-02-09 | Pelikan Technologies Inc. | Apparatus for improving success rate of blood yield from a fingerstick |
US7025774B2 (en) | 2001-06-12 | 2006-04-11 | Pelikan Technologies, Inc. | Tissue penetration device |
US7749174B2 (en) | 2001-06-12 | 2010-07-06 | Pelikan Technologies, Inc. | Method and apparatus for lancet launching device intergrated onto a blood-sampling cartridge |
US9226699B2 (en) | 2002-04-19 | 2016-01-05 | Sanofi-Aventis Deutschland Gmbh | Body fluid sampling module with a continuous compression tissue interface surface |
US7981056B2 (en) | 2002-04-19 | 2011-07-19 | Pelikan Technologies, Inc. | Methods and apparatus for lancet actuation |
ATE485766T1 (en) | 2001-06-12 | 2010-11-15 | Pelikan Technologies Inc | ELECTRICAL ACTUATING ELEMENT FOR A LANCET |
DE60234598D1 (en) | 2001-06-12 | 2010-01-14 | Pelikan Technologies Inc | SELF-OPTIMIZING LANZET DEVICE WITH ADAPTANT FOR TEMPORAL FLUCTUATIONS OF SKIN PROPERTIES |
US7682318B2 (en) | 2001-06-12 | 2010-03-23 | Pelikan Technologies, Inc. | Blood sampling apparatus and method |
US9795747B2 (en) | 2010-06-02 | 2017-10-24 | Sanofi-Aventis Deutschland Gmbh | Methods and apparatus for lancet actuation |
US9427532B2 (en) | 2001-06-12 | 2016-08-30 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
US7226461B2 (en) | 2002-04-19 | 2007-06-05 | Pelikan Technologies, Inc. | Method and apparatus for a multi-use body fluid sampling device with sterility barrier release |
US7297122B2 (en) | 2002-04-19 | 2007-11-20 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US7717863B2 (en) | 2002-04-19 | 2010-05-18 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US7175642B2 (en) | 2002-04-19 | 2007-02-13 | Pelikan Technologies, Inc. | Methods and apparatus for lancet actuation |
US7547287B2 (en) | 2002-04-19 | 2009-06-16 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US7674232B2 (en) | 2002-04-19 | 2010-03-09 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US7291117B2 (en) | 2002-04-19 | 2007-11-06 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US7371247B2 (en) | 2002-04-19 | 2008-05-13 | Pelikan Technologies, Inc | Method and apparatus for penetrating tissue |
US7901362B2 (en) | 2002-04-19 | 2011-03-08 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US7976476B2 (en) | 2002-04-19 | 2011-07-12 | Pelikan Technologies, Inc. | Device and method for variable speed lancet |
US7232451B2 (en) | 2002-04-19 | 2007-06-19 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US9248267B2 (en) | 2002-04-19 | 2016-02-02 | Sanofi-Aventis Deustchland Gmbh | Tissue penetration device |
US7229458B2 (en) | 2002-04-19 | 2007-06-12 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US8579831B2 (en) | 2002-04-19 | 2013-11-12 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
US8221334B2 (en) | 2002-04-19 | 2012-07-17 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
US7648468B2 (en) | 2002-04-19 | 2010-01-19 | Pelikon Technologies, Inc. | Method and apparatus for penetrating tissue |
US8784335B2 (en) | 2002-04-19 | 2014-07-22 | Sanofi-Aventis Deutschland Gmbh | Body fluid sampling device with a capacitive sensor |
US7491178B2 (en) | 2002-04-19 | 2009-02-17 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US9314194B2 (en) | 2002-04-19 | 2016-04-19 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
US7331931B2 (en) | 2002-04-19 | 2008-02-19 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US8267870B2 (en) | 2002-04-19 | 2012-09-18 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for body fluid sampling with hybrid actuation |
US7909778B2 (en) | 2002-04-19 | 2011-03-22 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US9795334B2 (en) | 2002-04-19 | 2017-10-24 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
US8702624B2 (en) | 2006-09-29 | 2014-04-22 | Sanofi-Aventis Deutschland Gmbh | Analyte measurement device with a single shot actuator |
US7892183B2 (en) | 2002-04-19 | 2011-02-22 | Pelikan Technologies, Inc. | Method and apparatus for body fluid sampling and analyte sensing |
US8574895B2 (en) | 2002-12-30 | 2013-11-05 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus using optical techniques to measure analyte levels |
DK1633235T3 (en) | 2003-06-06 | 2014-08-18 | Sanofi Aventis Deutschland | Apparatus for sampling body fluid and detecting analyte |
WO2006001797A1 (en) | 2004-06-14 | 2006-01-05 | Pelikan Technologies, Inc. | Low pain penetrating |
US8282576B2 (en) | 2003-09-29 | 2012-10-09 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for an improved sample capture device |
EP1680014A4 (en) | 2003-10-14 | 2009-01-21 | Pelikan Technologies Inc | Method and apparatus for a variable user interface |
EP1706026B1 (en) | 2003-12-31 | 2017-03-01 | Sanofi-Aventis Deutschland GmbH | Method and apparatus for improving fluidic flow and sample capture |
US7822454B1 (en) | 2005-01-03 | 2010-10-26 | Pelikan Technologies, Inc. | Fluid sampling device with improved analyte detecting member configuration |
US8828203B2 (en) | 2004-05-20 | 2014-09-09 | Sanofi-Aventis Deutschland Gmbh | Printable hydrogels for biosensors |
EP1756557B1 (en) * | 2004-05-21 | 2017-03-15 | Agamatrix, Inc. | Method of making an electrochemical cell |
EP1765194A4 (en) | 2004-06-03 | 2010-09-29 | Pelikan Technologies Inc | Method and apparatus for a fluid sampling device |
US8652831B2 (en) | 2004-12-30 | 2014-02-18 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for analyte measurement test time |
US7713392B2 (en) | 2005-04-15 | 2010-05-11 | Agamatrix, Inc. | Test strip coding and quality measurement |
DE502006004037D1 (en) | 2005-12-19 | 2009-07-30 | Roche Diagnostics Gmbh | SANDWICH SENSOR TO DETERMINE AN ANALYTIC CONCENTRATION |
EP1797817A1 (en) * | 2005-12-19 | 2007-06-20 | F.Hoffmann-La Roche Ag | Sandwich sensor for determining the concentration of an analyte |
EP1986923A4 (en) * | 2006-02-23 | 2012-12-19 | Agamatrix Inc | Used test strip storage container |
US8257651B2 (en) * | 2006-03-16 | 2012-09-04 | Agamatrix, Inc. | Analyte meter with rotatable user interface |
US8057659B2 (en) * | 2006-06-27 | 2011-11-15 | Agamatrix, Inc. | Detection of analytes in a dual-mediator electrochemical test strip |
US20080134806A1 (en) * | 2006-12-06 | 2008-06-12 | Agamatrix, Inc. | Container system for dispensing a liquid |
US7802467B2 (en) | 2006-12-22 | 2010-09-28 | Abbott Diabetes Care Inc. | Analyte sensors and methods of use |
US20080169799A1 (en) * | 2007-01-12 | 2008-07-17 | Shiow-Chen Wang | Method for biosensor analysis |
ES2683852T3 (en) * | 2007-01-23 | 2018-09-28 | Ascensia Diabetes Care Holdings Ag | Analyte test device |
KR20150013343A (en) * | 2007-07-23 | 2015-02-04 | 아가매트릭스, 인코포레이티드 | Electrochemical test strip |
AU2008279042B2 (en) | 2007-07-26 | 2014-03-13 | Agamatrix, Inc. | Electrochemical analyte detection apparatus and method |
WO2009015292A1 (en) * | 2007-07-26 | 2009-01-29 | Agamatrix, Inc. | Electrochemical test strips |
US20090208816A1 (en) * | 2008-01-31 | 2009-08-20 | Viavattine Joseph J | Properly positioning stacked plate electrode for high volume assembly |
WO2009126900A1 (en) | 2008-04-11 | 2009-10-15 | Pelikan Technologies, Inc. | Method and apparatus for analyte detecting device |
US8097926B2 (en) | 2008-10-07 | 2012-01-17 | Mc10, Inc. | Systems, methods, and devices having stretchable integrated circuitry for sensing and delivering therapy |
US9123614B2 (en) | 2008-10-07 | 2015-09-01 | Mc10, Inc. | Methods and applications of non-planar imaging arrays |
US8389862B2 (en) | 2008-10-07 | 2013-03-05 | Mc10, Inc. | Extremely stretchable electronics |
US9375169B2 (en) | 2009-01-30 | 2016-06-28 | Sanofi-Aventis Deutschland Gmbh | Cam drive for managing disposable penetrating member actions with a single motor and motor and control system |
US8758583B2 (en) * | 2009-04-28 | 2014-06-24 | Abbott Diabetes Care Inc. | Smart sensor ports and methods of using same |
US8828330B2 (en) | 2010-01-28 | 2014-09-09 | Abbott Diabetes Care Inc. | Universal test strip port |
US8721850B2 (en) * | 2010-02-02 | 2014-05-13 | Roche Diagnostics Operations, Inc. | Biosensor and methods for manufacturing |
US8965476B2 (en) | 2010-04-16 | 2015-02-24 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
KR101837074B1 (en) | 2010-07-07 | 2018-03-09 | 아가매트릭스, 인코포레이티드 | Analyte test strip and analyte meter device |
KR20130075776A (en) * | 2010-09-17 | 2013-07-05 | 아가매트릭스, 인코포레이티드 | Method and apparatus for encoding test strips |
US9046233B2 (en) * | 2010-09-27 | 2015-06-02 | Au Optronics Corporation | Assemblage structure for OLED lighting modules |
JP6210980B2 (en) | 2011-06-23 | 2017-10-11 | モレキュラー レバー デザイン,エルエルシー | Lithium ion battery using discrete carbon nanotubes, method for producing the same, and product obtained therefrom |
US10760111B2 (en) | 2011-07-27 | 2020-09-01 | Agamatrix, Inc. | Reagents for electrochemical test strips |
US9217723B2 (en) | 2012-03-02 | 2015-12-22 | Cilag Gmbh International | Co-facial analytical test strip with stacked unidirectional contact pads |
US20130228475A1 (en) * | 2012-03-02 | 2013-09-05 | Cilag Gmbh International | Co-facial analytical test strip with stacked unidirectional contact pads and inert carrier substrate |
SI2679156T1 (en) | 2012-06-28 | 2020-01-31 | F. Hoffmann-La Roche Ag | Device for monitoring at least one body function of a user and method for manufacturing the same |
KR20150072415A (en) | 2012-10-09 | 2015-06-29 | 엠씨10, 인크 | Conformal electronics integrated with apparel |
US9171794B2 (en) | 2012-10-09 | 2015-10-27 | Mc10, Inc. | Embedding thin chips in polymer |
US9157882B2 (en) | 2012-12-20 | 2015-10-13 | Cilag Gmbh International | Analytical test strip |
US9706647B2 (en) | 2013-05-14 | 2017-07-11 | Mc10, Inc. | Conformal electronics including nested serpentine interconnects |
US9354194B2 (en) * | 2013-06-19 | 2016-05-31 | Cilag Gmbh International | Orientation independent meter |
US20150047976A1 (en) * | 2013-08-16 | 2015-02-19 | Cilag Gmbh International | Analytical test strip having cantilevered contacts |
TWM473191U (en) * | 2013-09-12 | 2014-03-01 | Jun-Tong Chen | Test strip for detection |
US20150096906A1 (en) * | 2013-10-07 | 2015-04-09 | Cilag Gmbh International | Biosensor with bypass electrodes |
CN105813545A (en) | 2013-11-22 | 2016-07-27 | Mc10股份有限公司 | Conformal sensor systems for sensing and analysis of cardiac activity |
US20150144507A1 (en) * | 2013-11-22 | 2015-05-28 | Cilag Gmbh International | Folded biosensor |
US9500616B2 (en) * | 2013-12-23 | 2016-11-22 | Cilag Gmbh International | Multi-orientation test strip |
JP6637896B2 (en) | 2014-03-04 | 2020-01-29 | エムシー10 インコーポレイテッドMc10,Inc. | Conformal IC device with flexible multi-part encapsulated housing for electronic devices |
CA2955198C (en) * | 2014-07-17 | 2017-10-10 | Siemens Healthcare Diagnostics Inc. | Sensor array |
USD781270S1 (en) | 2014-10-15 | 2017-03-14 | Mc10, Inc. | Electronic device having antenna |
CN107530004A (en) | 2015-02-20 | 2018-01-02 | Mc10股份有限公司 | The automatic detection and construction of wearable device based on personal situation, position and/or orientation |
US9788317B2 (en) | 2015-03-30 | 2017-10-10 | Intel IP Corporation | Access point (AP), user station (STA) and method for channel sounding using sounding trigger frames |
CN104967995A (en) * | 2015-06-30 | 2015-10-07 | 北京奇虎科技有限公司 | Method for acquiring WIFI network password, client and server |
WO2017031129A1 (en) | 2015-08-19 | 2017-02-23 | Mc10, Inc. | Wearable heat flux devices and methods of use |
CN108290070A (en) | 2015-10-01 | 2018-07-17 | Mc10股份有限公司 | Method and system for interacting with virtual environment |
EP3359031A4 (en) | 2015-10-05 | 2019-05-22 | Mc10, Inc. | Method and system for neuromodulation and stimulation |
EP3420733A4 (en) | 2016-02-22 | 2019-06-26 | Mc10, Inc. | System, device, and method for coupled hub and sensor node on-body acquisition of sensor information |
EP3420732B8 (en) | 2016-02-22 | 2020-12-30 | Medidata Solutions, Inc. | System, devices, and method for on-body data and power transmission |
CN109310340A (en) | 2016-04-19 | 2019-02-05 | Mc10股份有限公司 | For measuring the method and system of sweat |
US10447347B2 (en) | 2016-08-12 | 2019-10-15 | Mc10, Inc. | Wireless charger and high speed data off-loader |
IT201600126012A1 (en) * | 2016-12-14 | 2018-06-14 | Nanomaterials It S R L | Miniaturized electrochemical cell. |
JP7142642B2 (en) * | 2017-03-21 | 2022-09-27 | エフ ホフマン-ラ ロッシュ アクチェン ゲゼルシャフト | Medical device and method for manufacturing a medical device |
WO2023014365A1 (en) * | 2021-08-05 | 2023-02-09 | Hewlett-Packard Development Company, L.P. | Polymerase chain reaction test well including resistive sheet |
WO2023014364A1 (en) * | 2021-08-05 | 2023-02-09 | Hewlett-Packard Development Company, L.P. | Polymerase chain reaction well including a side wall with a fluoropolymer |
Family Cites Families (88)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS55500431A (en) * | 1978-08-15 | 1980-07-17 | ||
DE3278334D1 (en) * | 1981-10-23 | 1988-05-19 | Genetics Int Inc | Sensor for components of a liquid mixture |
DE3221339A1 (en) * | 1982-06-05 | 1983-12-08 | Basf Ag, 6700 Ludwigshafen | METHOD FOR THE ELECTROCHEMICAL HYDRATION OF NICOTINAMIDADENINE-DINUCLEOTIDE |
CA1219040A (en) * | 1983-05-05 | 1987-03-10 | Elliot V. Plotkin | Measurement of enzyme-catalysed reactions |
CA1261256A (en) * | 1984-06-13 | 1989-09-26 | Ian A. Shanks | Devices for use in chemical test procedures |
EP0359831B2 (en) * | 1988-03-31 | 2007-06-20 | Matsushita Electric Industrial Co., Ltd. | Biosensor and process for its production |
US4942127A (en) * | 1988-05-06 | 1990-07-17 | Molecular Devices Corporation | Polyredox couples in analyte determinations |
US5286362A (en) * | 1990-02-03 | 1994-02-15 | Boehringer Mannheim Gmbh | Method and sensor electrode system for the electrochemical determination of an analyte or an oxidoreductase as well as the use of suitable compounds therefor |
FR2673289B1 (en) | 1991-02-21 | 1994-06-17 | Asulab Sa | SENSOR FOR MEASURING THE QUANTITY OF A COMPONENT IN SOLUTION. |
DE4123348A1 (en) * | 1991-07-15 | 1993-01-21 | Boehringer Mannheim Gmbh | ELECTROCHEMICAL ANALYSIS SYSTEM |
US5264103A (en) * | 1991-10-18 | 1993-11-23 | Matsushita Electric Industrial Co., Ltd. | Biosensor and a method for measuring a concentration of a substrate in a sample |
JP2658769B2 (en) * | 1991-10-21 | 1997-09-30 | 松下電器産業株式会社 | Biosensor |
US5710011A (en) * | 1992-06-05 | 1998-01-20 | Medisense, Inc. | Mediators to oxidoreductase enzymes |
US5845702A (en) * | 1992-06-30 | 1998-12-08 | Heat Pipe Technology, Inc. | Serpentine heat pipe and dehumidification application in air conditioning systems |
FR2699170B1 (en) * | 1992-12-15 | 1995-07-28 | Asulab Sa | Complexes of a transition metal with 2,2'-bipyridine ligands substituted by at least one alkyl ammonium radical, their manufacturing process and their use as redox mediator. |
CA2153883C (en) * | 1993-06-08 | 1999-02-09 | Bradley E. White | Biosensing meter which detects proper electrode engagement and distinguishes sample and check strips |
FR2710413B1 (en) * | 1993-09-21 | 1995-11-03 | Asulab Sa | Measuring device for removable sensors. |
US5589326A (en) | 1993-12-30 | 1996-12-31 | Boehringer Mannheim Corporation | Osmium-containing redox mediator |
EP0752099A1 (en) * | 1994-02-09 | 1997-01-08 | Abbott Laboratories | Diagnostic flow cell device |
US5437999A (en) | 1994-02-22 | 1995-08-01 | Boehringer Mannheim Corporation | Electrochemical sensor |
US5695947A (en) * | 1995-06-06 | 1997-12-09 | Biomedix, Inc. | Amperometric cholesterol biosensor |
AUPN363995A0 (en) | 1995-06-19 | 1995-07-13 | Memtec Limited | Electrochemical cell |
AUPN661995A0 (en) | 1995-11-16 | 1995-12-07 | Memtec America Corporation | Electrochemical cell 2 |
US6638415B1 (en) * | 1995-11-16 | 2003-10-28 | Lifescan, Inc. | Antioxidant sensor |
US6174420B1 (en) * | 1996-11-15 | 2001-01-16 | Usf Filtration And Separations Group, Inc. | Electrochemical cell |
JP3365184B2 (en) * | 1996-01-10 | 2003-01-08 | 松下電器産業株式会社 | Biosensor |
JP3913289B2 (en) * | 1996-06-14 | 2007-05-09 | セラセンス インコーポレーテッド | Glucose biosensor |
JP3460183B2 (en) * | 1996-12-24 | 2003-10-27 | 松下電器産業株式会社 | Biosensor |
DE69809391T2 (en) * | 1997-02-06 | 2003-07-10 | Therasense Inc | SMALL VOLUME SENSOR FOR IN-VITRO DETERMINATION |
AUPO585797A0 (en) * | 1997-03-25 | 1997-04-24 | Memtec America Corporation | Improved electrochemical cell |
GB9824627D0 (en) | 1998-11-11 | 1999-01-06 | Cambridge Sensors Ltd | Test strips for small volumes |
EP2006669A3 (en) * | 1997-07-22 | 2008-12-31 | Kyoto Daiichi Kagaku Co., Ltd. | Concentration measuring apparatus and test strip for the concentration measuring apparatus |
JP3528529B2 (en) * | 1997-07-31 | 2004-05-17 | Nok株式会社 | Biosensor |
US6129823A (en) * | 1997-09-05 | 2000-10-10 | Abbott Laboratories | Low volume electrochemical sensor |
US6071391A (en) * | 1997-09-12 | 2000-06-06 | Nok Corporation | Enzyme electrode structure |
US5876952A (en) * | 1997-12-08 | 1999-03-02 | Shieh; Paul | Non-invasive glucose biosensor: determination of glucose in urine |
JP3978489B2 (en) * | 1998-02-26 | 2007-09-19 | アークレイ株式会社 | Blood measuring device |
US6878251B2 (en) * | 1998-03-12 | 2005-04-12 | Lifescan, Inc. | Heated electrochemical cell |
US6294062B1 (en) * | 1998-06-01 | 2001-09-25 | Roche Diagnostics Corporation | Method and device for electrochemical immunoassay of multiple analytes |
JP3874321B2 (en) | 1998-06-11 | 2007-01-31 | 松下電器産業株式会社 | Biosensor |
US6338790B1 (en) | 1998-10-08 | 2002-01-15 | Therasense, Inc. | Small volume in vitro analyte sensor with diffusible or non-leachable redox mediator |
US6258229B1 (en) * | 1999-06-02 | 2001-07-10 | Handani Winarta | Disposable sub-microliter volume sensor and method of making |
US6193873B1 (en) | 1999-06-15 | 2001-02-27 | Lifescan, Inc. | Sample detection to initiate timing of an electrochemical assay |
JP2001021528A (en) | 1999-07-02 | 2001-01-26 | Akebono Brake Res & Dev Center Ltd | Electrode type biosensor |
US7045054B1 (en) * | 1999-09-20 | 2006-05-16 | Roche Diagnostics Corporation | Small volume biosensor for continuous analyte monitoring |
US6616819B1 (en) * | 1999-11-04 | 2003-09-09 | Therasense, Inc. | Small volume in vitro analyte sensor and methods |
US6923894B2 (en) * | 1999-11-11 | 2005-08-02 | Apex Biotechnology Corporation | Biosensor with multiple sampling ways |
CN1632553A (en) * | 1999-11-15 | 2005-06-29 | 松下电器产业株式会社 | Biosensor, method of forming thin-film electrode, and method and apparatus for quantitative determination |
AU1607801A (en) * | 1999-11-15 | 2001-05-30 | Therasense, Inc. | Transition metal complexes with bidentate ligand having an imidazole ring |
US6562210B1 (en) * | 1999-12-30 | 2003-05-13 | Roche Diagnostics Corporation | Cell for electrochemical anaylsis of a sample |
AU2001228915A1 (en) * | 2000-03-22 | 2001-10-03 | All Medicus Co., Ltd. | Electrochemical biosensor test strip with recognition electrode and readout meter using this test strip |
DK1269173T3 (en) | 2000-03-28 | 2005-11-14 | Diabetes Diagnostics Inc | Glucose sensor with fast response |
WO2001075438A2 (en) * | 2000-03-31 | 2001-10-11 | Lifescan, Inc. | Electrically-conductive patterns for monitoring the filling of medical devices |
US6488827B1 (en) * | 2000-03-31 | 2002-12-03 | Lifescan, Inc. | Capillary flow control in a medical diagnostic device |
EP1167538A1 (en) * | 2000-06-30 | 2002-01-02 | Schibli Engineering GmbH | Biosensor and method for its production |
SG140463A1 (en) * | 2000-07-14 | 2008-03-28 | Lifescan Inc | Electrochemical method for measuring chemical reaction rates |
US20020035188A1 (en) * | 2000-07-21 | 2002-03-21 | Steeghs Henricus Renier Gerardus | Agglomerating particulate materials |
US6726818B2 (en) * | 2000-07-21 | 2004-04-27 | I-Sens, Inc. | Biosensors with porous chromatographic membranes |
JP4177662B2 (en) | 2000-07-24 | 2008-11-05 | 松下電器産業株式会社 | Biosensor |
US6447657B1 (en) * | 2000-12-04 | 2002-09-10 | Roche Diagnostics Corporation | Biosensor |
US6558528B1 (en) * | 2000-12-20 | 2003-05-06 | Lifescan, Inc. | Electrochemical test strip cards that include an integral dessicant |
US6572745B2 (en) * | 2001-03-23 | 2003-06-03 | Virotek, L.L.C. | Electrochemical sensor and method thereof |
DE10117868A1 (en) * | 2001-04-10 | 2002-10-17 | Lre Technology Partner Gmbh | Test strip, for the electrical measurement of substance concentrations in body fluid samples, has parallel outer layers with electrodes on their inner surfaces, and an absorbent intermediate layer with a specific reagent at the layers |
US6855243B2 (en) * | 2001-04-27 | 2005-02-15 | Lifescan, Inc. | Electrochemical test strip having a plurality of reaction chambers and methods for using the same |
EP1452857A4 (en) * | 2001-11-14 | 2006-05-24 | Matsushita Electric Ind Co Ltd | Biosensor |
AU2002356956A1 (en) * | 2001-11-16 | 2003-06-10 | North Carolina State University | Biomedical electrochemical sensor array and method of fabrication |
US6749887B1 (en) * | 2001-11-28 | 2004-06-15 | Lifescan, Inc. | Solution drying system |
US6689411B2 (en) * | 2001-11-28 | 2004-02-10 | Lifescan, Inc. | Solution striping system |
KR100475634B1 (en) | 2001-12-24 | 2005-03-15 | 주식회사 아이센스 | Biosensor equipped with sample introducing part which enables quick introduction of a small amount of sample |
US6946067B2 (en) * | 2002-01-04 | 2005-09-20 | Lifescan, Inc. | Method of forming an electrical connection between an electrochemical cell and a meter |
US7601249B2 (en) | 2002-02-10 | 2009-10-13 | Agamatrix, Inc. | Method and apparatus for assay of electrochemical properties |
US6881578B2 (en) * | 2002-04-02 | 2005-04-19 | Lifescan, Inc. | Analyte concentration determination meters and methods of using the same |
US6676101B2 (en) * | 2002-05-28 | 2004-01-13 | Minus K. Technology, Inc. | Vibration isolation system |
US6732501B2 (en) | 2002-06-26 | 2004-05-11 | Heartware, Inc. | Ventricular connector |
JP2004024764A (en) | 2002-06-28 | 2004-01-29 | Brother Ind Ltd | Sewing apparatus, thread cassette of sewing apparatus, and program of sewing apparatus |
DE60324738D1 (en) | 2002-07-18 | 2009-01-02 | Panasonic Corp | Measuring device with a biosensor |
EP1396717A1 (en) * | 2002-09-03 | 2004-03-10 | Matsushita Electric Industrial Co., Ltd. | Biosensor and measuring method using the same |
US7291256B2 (en) * | 2002-09-12 | 2007-11-06 | Lifescan, Inc. | Mediator stabilized reagent compositions and methods for their use in electrochemical analyte detection assays |
US7771575B2 (en) * | 2002-10-25 | 2010-08-10 | Arkray, Inc. | Analytical tool |
CN1453579A (en) * | 2003-05-19 | 2003-11-05 | 浙江大学 | Electrochemical blood lactic acid sensor |
MXPA06001914A (en) | 2003-08-21 | 2006-05-31 | Agamatrix Inc | Method and apparatus for assay of electrochemical properties. |
US7387714B2 (en) * | 2003-11-06 | 2008-06-17 | 3M Innovative Properties Company | Electrochemical sensor strip |
WO2005108968A1 (en) | 2004-05-12 | 2005-11-17 | Matsushita Electric Industrial Co., Ltd. | Biosensor, container for biosensor, and biosensor measuring apparatus |
EP1756557B1 (en) * | 2004-05-21 | 2017-03-15 | Agamatrix, Inc. | Method of making an electrochemical cell |
US7556723B2 (en) * | 2004-06-18 | 2009-07-07 | Roche Diagnostics Operations, Inc. | Electrode design for biosensor |
US7547382B2 (en) | 2005-04-15 | 2009-06-16 | Agamatrix, Inc. | Determination of partial fill in electrochemical strips |
US20070017824A1 (en) * | 2005-07-19 | 2007-01-25 | Rippeth John J | Biosensor and method of manufacture |
US9535027B2 (en) * | 2012-07-25 | 2017-01-03 | Abbott Diabetes Care Inc. | Analyte sensors and methods of using same |
-
2005
- 2005-05-20 EP EP05739481.9A patent/EP1756557B1/en active Active
- 2005-05-20 CN CN201310269372.XA patent/CN103353475B/en active Active
- 2005-05-20 AU AU2005246102A patent/AU2005246102B8/en active Active
- 2005-05-20 KR KR1020137014837A patent/KR101328608B1/en active IP Right Grant
- 2005-05-20 EP EP16163412.6A patent/EP3059580A1/en active Pending
- 2005-05-20 CA CA2566358A patent/CA2566358C/en active Active
- 2005-05-20 WO PCT/IB2005/051657 patent/WO2005114159A1/en active Application Filing
- 2005-05-20 CN CN201210239381.XA patent/CN102778484B/en active Active
- 2005-05-20 CN CN200580024249.5A patent/CN101014851B/en active Active
- 2005-05-20 CN CN201410219207.8A patent/CN103954668B/en active Active
- 2005-05-20 JP JP2007517568A patent/JP5215661B2/en active Active
- 2005-05-20 KR KR1020127034142A patent/KR101330785B1/en active IP Right Grant
- 2005-05-20 US US10/908,656 patent/US8268145B2/en active Active
- 2005-05-21 WO PCT/IB2005/051660 patent/WO2005116622A1/en active Application Filing
- 2005-05-21 CA CA002566214A patent/CA2566214A1/en not_active Abandoned
- 2005-05-21 CN CNA2005800162607A patent/CN1985001A/en active Pending
- 2005-05-21 CN CNA2005800163239A patent/CN1957249A/en active Pending
- 2005-05-21 ES ES05740441.0T patent/ES2569059T3/en active Active
- 2005-05-21 CA CA002565523A patent/CA2565523A1/en not_active Abandoned
- 2005-05-21 ES ES05740440T patent/ES2350489T3/en active Active
- 2005-05-21 PT PT05740440T patent/PT1747281E/en unknown
- 2005-05-21 US US10/908,664 patent/US8617365B2/en active Active
- 2005-05-21 JP JP2007517570A patent/JP2008500550A/en active Pending
- 2005-05-21 AU AU2005245694A patent/AU2005245694B2/en active Active
- 2005-05-21 US US10/908,662 patent/US7527716B2/en active Active
- 2005-05-21 KR KR1020067027006A patent/KR20070026634A/en not_active Application Discontinuation
- 2005-05-21 AU AU2005248556A patent/AU2005248556C1/en active Active
- 2005-05-21 JP JP2007517571A patent/JP2008500551A/en active Pending
- 2005-05-21 DE DE602005023435T patent/DE602005023435D1/en active Active
- 2005-05-21 EP EP05740441.0A patent/EP1747450B1/en active Active
- 2005-05-21 EP EP05740440A patent/EP1747281B1/en active Active
- 2005-05-21 KR KR1020067024297A patent/KR101224499B1/en active IP Right Grant
- 2005-05-21 AT AT05740440T patent/ATE480636T1/en active
- 2005-05-21 WO PCT/IB2005/051659 patent/WO2005113790A1/en not_active Application Discontinuation
-
2006
- 2006-12-20 NO NO20065935A patent/NO20065935L/en unknown
- 2006-12-20 NO NO20065933A patent/NO20065933L/en unknown
- 2006-12-20 NO NO20065934A patent/NO20065934L/en unknown
- 2006-12-21 KR KR1020067027005A patent/KR101265504B1/en active IP Right Grant
-
2008
- 2008-02-01 HK HK13105252.7A patent/HK1178598A1/en unknown
- 2008-02-01 HK HK08101273.8A patent/HK1111217A1/en unknown
-
2010
- 2010-08-20 AU AU2010212483A patent/AU2010212483B2/en active Active
- 2010-09-29 AU AU2010224472A patent/AU2010224472B2/en active Active
-
2011
- 2011-03-21 US US13/052,605 patent/US8182636B2/en active Active
-
2012
- 2012-05-29 AU AU2012203146A patent/AU2012203146B2/en active Active
- 2012-08-14 US US13/584,912 patent/US20120305396A1/en not_active Abandoned
-
2013
- 2013-08-27 US US14/010,792 patent/US11175256B2/en active Active
-
2014
- 2014-05-30 US US14/291,990 patent/US9329150B2/en active Active
-
2015
- 2015-01-27 HK HK15100870.8A patent/HK1200532A1/en unknown
-
2016
- 2016-05-02 US US15/144,024 patent/US10203298B2/en active Active
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP1747281B1 (en) | Electrochemical assay device and related methods | |
US6911621B2 (en) | Biosensor | |
CA2328535C (en) | Biosensor apparatus | |
US20040224369A1 (en) | Disposable sensor with enhanced sample port inlet | |
KR19990028415A (en) | Electrochemical Biosensor Specimen | |
Bhullar et al. | Biosensor | |
AU2015200854A1 (en) | Electrochemical cell and method of making an electrochemical cell |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Discontinued |