CA2598695A1 - Hydroxyalkyl substituted imidazoquinolines - Google Patents

Abstract

Certain imidazoquinolines with a hydroxymethyl or hydroxyethyl substituent at the 2-position, pharmaceutical compositions containing the compounds, intermediates, methods of making and methods of use of these compounds as immunomodulators, for preferentially inducing IFN-.alpha. biosynthesis in animals and in the treatment of diseases including viral and neoplastic diseases are disclosed.

Description

HYDROXYALKYL SUBSTITUTED IMIDAZOQUINOLINES

CROSS REFERENCE TO RELATED APPLICATIONS
The present invention claims priority to U.S. Provisional Application Serial No.
60/655380, filed February 23, 2005, which is incorporated herein by reference.
BACKGROUND
Certain compounds have been found to be useful as immune response modifiers (IRMs), rendering them useful in the treatment of a variety of disorders.
However, there continues to be interest in and a need for compounds that have the ability to modulate the immune response, by induction of cytokine biosynthesis or other means.

SUMMARY
The present invention provides a new class of compounds which preferentially induce the biosynthesis of interferon (a) (IFN-a) in animals. Such compounds are of the following Formulas I, II, and III:

N N
\>--(CH2)õOH
N

(R)m I
A
HN
N N
\>-(CH2)r,OH
N

Ri (R)m II

NHZ

N N
\>-(CH2)no _G2 N
R, ~R)m III
wherein R, Ri, G1, Ga, m, and n are as defined below.
It has now surprisingly been discovered that the amount of TNF-a induced by the 2-(hydroxyalkyl) substituted compounds of the invention is substantially less than the amount of TNF-a induced by closely related analogs having an alkyl or alkyl ether substituent at the 2-position and that the compounds of the invention still retain the ability to induce the biosynthesis of IFN-a. See, for example, Figures 1-4 below. The reduction in the amount of TNF-a induced is seen over a broad range of test concentrations. In some embodiments the amount of TNF-a induced by the compounds of the invention is at least two-fold less than the amount of TNF-a induced by analogs having an alkyl or alkyl ether substituent at the 2-position. In other embodiments the amount of TNF-a induced by the compounds of the invention is at least three-fold less than the amount of TNF-a induced by analogs having an alkyl or alkyl ether substituent at the 2-position. In still other embodiments the amount of TNF-a induced by the compounds of the invention is at least four-fold less than the amount of TNF-a induced by analogs having an alkyl or alkyl ether substituent at the 2-position.
As used herein "substantially less than the amount of TNF-a " means that there is at least a two-fold reduction in the maximal TNF-a response as determined using the test methods described herein.

The compounds or salts of Formulas I, II, and III are especially useful as immune response modifiers due to their ability to preferentially induce interferon-a, thus providing a benefit over compounds that also induce pro-inflammatory cytokines (e.g.
TNF-a) or that induce pro-inflammatory cytokines at higher levels.
A compound is said to preferentially induce IFN-a if, when tested according to the test methods described herein, the effective minimum concentration for IFN-a induction is less than the effective minimum concentration for TNF-a induction. In some embodiments, the effective minimum concentration for IFN-a induction is at least 3-fold less than the effective minimum concentration for TNF-a induction. In some embodiments, the effective minimum concentration for IFN-a induction is at least 6-fold less than the effective minimum concentration for TNF-a induction. In other embodiments, the effective minimum concentration for IFN-a induction is at least 9-fold less than the effective minimum concentration for TNF-a induction. In some embodiments, when tested according to the test methods described herein, the amount TNF-a induced by compounds of the invention is at or below the background level of TNF-a in the test method.
The invention further provides pharmaceutical compositions containing an effective amount of a compound or salt of Formulas I, II, and/or III and methods of preferentially inducing the biosynthesis of IFN-a in an animal, and treating a viral infection or disease and/or treating a neoplastic disease in an animal by administering an effective amount of a compound or salt of Formulas I, II, and/or III or a pharmaceutical composition containing an effective amount of a compound or salt of Forinulas I, II, and/or III to the animal.
In addition, methods of synthesizing compounds of Formulas I, II, and III and intermediates useful in the synthesis of these compounds are provided.
As used herein, "a," "an," "the," "at least one," and "one or more" are used interchangeably.
The terms "comprises" and variations thereof do not have a limiting meaning where these terms appear in the description and claims.
The above summary of the present invention is not intended to describe each disclosed embodiment or every implementation of the present invention. The description that follows more particularly exemplifies illustrative embodiments. In several places throughout the description, guidance is provided through lists of examples, which examples can be used in various combinations. In each instance, the recited list serves only as a representative group and should not be interpreted as an exclusive list.
BRIEF DESCRIPTION OF THE DRAWINGS
Figure 1 shows the IFN-a dose response curves (corresponding to values shown in Table 5 below) for Example 6, Analog 2, Analog 3, and Analog 5.
Figure 2 shows the TNF-a dose response curves (corresponding to values shown in Table 5 below) for Example 6, Analog 2, Analog 3, and Analog 5.

Figure 3 shows the IFN-a dose response curves (corresponding to values shown in Table 5 below) for Example 7, Analog 1, Analog 2, and Analog 4.
Figure 4 shows the TNF-a dose response curves (corresponding to values shown in Table 5 below) for Example 7, Analog 1, Analog 2, and Analog 4.
DETAILED DESCRIPTION OF ILLUSTRATIVE EMBODIMENTS OF THE
INVENTION
The present invention provides compounds of the following Formulas I, II, and III:

N
N \>--(CH2),,OH
N

Ra (R)m I
~Gi HN
N N
~}-(CH2)11OH
N

Ri (R)m II

N N
\>(CH2)110_G2 N

Ri (R)m III
wherein R, Rl, GI, G2, m, and n are as defined below; and pharmaceutically acceptable salts thereof.
In one embodiment, the present invention provides a compound of the following Formula I:

N
~--(CHx)~OH
NHtRi N
(R)I
wherein:
mis0or 1;
nislor2;
R is selected from the group consisting of Cl_3 0 alkyl, CI_10 alkoxy, halogen, and Ci_lo haloalkyl;
Rl is selected from the group consisting of:
-X-Y-R4, -X-R5, and -X-Het;
X is straight chain or branched chain alkylene optionally interrupted by one -group;
Y is selected from the group consisting of -S(0)0_2-and -N(Rg)-Q-;
R4 is selected from the group consisting of hydrogen, alkyl, alkenyl, aryl, arylalkylenyl, heteroaryl, and heteroarylalkylenyl, wherein the alkyl, alkenyl, aryl, arylalkylenyl, heteroaryl, and heteroarylalkylenyl, groups can be unsubstituted or substituted by one or more substituents independently selected from the group consisting of alkyl, alkoxy, hydroxyalkyl, haloalkyl, haloalkoxy, halogen, nitro, hydroxy, mercapto, cyano, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocyclyl, amino, alkylanlino, dialkylamino, and in the case of alkyl and alkenyl, oxo;
R5 is selected from the group consisting of:
(CH2)a -N-' C(Rs) --N- S(0)2 -N(Rg)-C(Rs)-N(- A
~ R71) R,~ -..(CH2)b-~/
and r(CH2)a-1 N-C(R6)--N A
~(CH
R2)b Het is selected from the group consisting of tetrahydropyranyl and tetrahydrofuranyl;
R6 is selected from the group consisting of =O and =S;
R7 is C2_7 alkylene;
R8 is selected from the group consisting of hydrogen, alkyl, alkoxyalkylenyl, hydroxyalkylenyl, arylalkylenyl, and heteroarylallcylenyl;
Rlo is C3_8 allcylene;
A is selected from the group consisting of -0-, -C(O)-, -CH2-, -S(O)0_2-, and -N(Q-R4)-;
Q is selected from the group consisting of a bond, -C(R6)-, -S(O)2, -C(R6)-N(R8)-, -S(O)Z-N(R8)-, -C(R6)-0-, and -C(R6)-S-; and a and b are independently integers from 1 to 6 with the proviso that a + b is < 7;
with the proviso that when Y is -S(O)0_2- then X can not contain an -0- group;
or a pharmaceutically acceptable salt thereof.
In another embodiment, the present invention provides a compound of the following Formula II, which is a prodrug:

A
HN

N \/\--(CH2),OH
N
"I, N
Ri (R),,, II
wherein:
Gl is selected from the group consisting of:
-C(O)-R', a-aminoacyl, a-aminoacyl-a-aminoacyl, -C(O)-O-R', -C(O)-N(R")R', -C(=NY')-R', -CH(OH)-C(O)-OY', -CH(OC1_4 alkyl)Yo, -CH2Y1a and -CH(CH3)Yl;
R' and R" are independently selected from the group consisting of C1_lo alkyl, C3_7 cycloalkyl, plienyl, benzyl, and 2-phenylethyl, each of which may be unsubstituted or substituted by one or more substituents independently selected from the group consisting of halogen, hydroxy, nitro, cyano, carboxy, C1_6 alkyl, C1_4 alkoxy, aryl, heteroaryl, aryl-C1_4 alkylenyl, heteroaryl-Ci_4 allcylenyl, halo-C1_4 alkylenyl, halo-C1 _4 alkoxy, -O-C(O)-CH3, -C(O)-O-CH3, -C(O)-NH2, -O-CH2-C(O)-NH2, -NH2, and -S(O)2 NHa, with the proviso that R" can also be hydrogen;
a-aminoacyl is an a-aminoacyl group derived from an a-amino acid selected from the group consisting of racemic, D-, and L-amino acids;
Y' is selected from the group consisting of hydrogen, C1_6 alkyl, and benzyl;
Yo is selected from the group consisting of C1_6 alkyl, carboxy-C1_6 alkylenyl, amino-C 1_4 alkylenyl, mono-N C 1.6 alkylamino-C 1_4 allcylenyl, and di-N,N-C1_6 alkylamino-C1_4 alkylenyl;
Y, is selected from the group consisting of mono-N-C1_6 alkylamino, di-N,N-C1_6 alkylamino, morpholin-4-yl, piperidin-l-yl, pyrrolidin-l-yl, and 4-C 1 -4 alkylpiperazin-l-yl;
mis0or1;
n is l or 2;
R is selected from the group consisting of C1_lo alkyl, Cl_lo alkoxy, halogen, and C1_10 haloalkyl;
Rl is selected from the group consisting of:
-X-Y-R4, -X-R5, and -X-Het;
X is straight chain or branched chain alkylene optionally interrupted by one -group;
Y is selected from the group consisting of -S(O)o_Z-and -N(R8)-Q-;
R4 is selected from the group consisting of hydrogen, alkyl, alkenyl, aryl, arylalkylenyl, heteroaryl, and heteroarylalkylenyl, wherein the alkyl, alkenyl, aryl, arylalkylenyl, heteroaryl, and heteroarylalkylenyl, groups can be unsubstituted or substituted by one or more substituents independently selected from the group consisting of alkyl, alkoxy, hydroxyallcyl, haloalkyl, haloalkoxy, halogen, nitro, hydroxy, mercapto, cyano, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocyclyl, amino, alkylamino, dialkylamino, and in the case of alkyl and alkenyl, oxo;
R5 is selected from the group consisting of:
~ (CHz)a C- ~ R6) -N S(0)2 -N(Re)-C(R6)-N A
R ~
R7 ~(CHa)n -/
and r(CHZ)a -'*~) N-C(R6)-N A
R~o '(CH2)b Het is selected from the group consisting of tetrahydropyranyl and tetrahydrofuranyl;
R6 is selected from the group consisting of =0 and =S;
R7 is C2_7 alkylene;
R8 is selected from the group consisting of hydrogen, alkyl, alkoxyalkylenyl, hydroxyalkylenyl, arylalkylenyl, and heteroarylalkylenyl;
Rlo is C3_$ alkylene;
A is selected from the group consisting of -0-, -C(O)-, -CH2-, -S(O)o_z-, and -N(Q-R4)-;
Q is selected from the group consisting of a bond, -C(R6)-, -S(O)a, -C(R6)-N(R8)-, -S(0)2-N(R8)-, -C(R6)-0-, and -C(R6)-S-; and a and b are independently integers from 1 to 6 with the proviso that a + b is < 7;
with the proviso that when Y is -S(O)0_2- then X can not contain an -0- group;
or a pharmaceutically acceptable salt thereof.
In another embodiment, the present invention provides a compound of the following Formula III, which is a prodrug:

N
\(CH2)n0-G2 N
PR, (R25 III

wherein:
G2 is selected from the group consisting of:
-X2-C(O)-R', a-aminoacyl, a-aminoacyl-a-aminoacyl, -X2-C(O)-O-R', and -C(O)-N(R")R';
X2 is selected from the group consisting of a bond; -CH2-0-; -CH(CH3)-0-;
-C(CH3)2-0-; and, in the case of -Xa-C(O)-O-R', -CH2-NH-;
R' and R" are independently selected from the group consisting of Cl_lo allcyl, C3-7 cycloalkyl, phenyl, benzyl, and 2-phenylethyl, each of which may be unsubstituted or substituted by one or more substituents independently selected from the group consisting of halogen, hydroxy, nitro, cyano, carboxy, C1 .6 alkyl, C1_4 alkoxy, aryl, heteroaryl, aryl-CI_4 alkylenyl, heteroaryl-C1_4 alkylenyl, halo-C1_4 alkylenyl, halo-C1_4 alkoxy, -O-C(O)-CH3, -C(O)-O-CH3, -C(O)-NH2, -O-CH2-C(O)-NH2, -NHZ, and -S(O)2-NHa, with the proviso that R" can also be hydrogen;
a-aminoacyl is an a-aminoacyl group derived from an a-amino acid selected from the group consisting of racemic, D-, and L-amino acids;
mis0or1;
n is l or 2;
F. is selected from the group consisting of CI_IO alkyl, C1_lo alkoxy, halogen, and C1_Io haloalkyl;
Rl is selected from the group consisting of:
-X-Y-R4, -X-R5, and -X-Het;
X is straight chain or branched chain alkylene optionally interrupted by one -group;
Y is selected from the group consisting of -S(O)o_2-and -N(R$)-Q-;
R4 is selected from the group consisting of hydrogen, alkyl, alkenyl, aryl, arylalkylenyl, heteroaryl, and heteroarylalkylenyl, wherein the alkyl, alkenyl, aryl, arylalkylenyl, heteroaryl, and heteroarylalkylenyl, groups can be unsubstituted or substituted by one or more substituents independently selected from the group consisting of alkyl, alkoxy, hydroxyalkyl, haloalkyl, haloalkoxy, halogen, nitro, hydroxy, mercapto, cyano, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocyclyl, amino, alkylamino, dialkylamino, and in the case of alkyl and alkenyl, oxo;
R5 is selected from the group consisting of:
~ (CHZ)a N ~ R6) - N- S(O)z -N(Ra)-C(R6)-N A
R7 ~ C R7J ~ (CH2)b-~
p ' and ~--(CH2)a --~
N-C(R6)-N A
RIo \(CHzb Het is selected from the group consisting of tetrahydropyranyl and tetrahydrofuranyl;
R6 is selected from the group consisting of =0 and =S;
R7 is C2_7 allcylene;
R8 is selected from the group consisting of hydrogen, alkyl, alkoxyalkylenyl, hydroxyalkylenyl, arylalkylenyl, and heteroarylalkylenyl;
Rlo is C3-8 alkylene;
A is selected from the group consisting of -0-, -C(O)-, -CH2-, -S(O)o_a-, and -N(Q-Ra)-;
Q is selected from the group consisting of a bond, -C(R6)-, -S(0)2, -C(R6)-N(R8)-, -S(0)2-N(R8)-, -C(R6)-0-, and -C(R6)-S-; and a and b are independently integers from 1 to 6 with the proviso that a + b is _< 7;
with the proviso that when Y is -S(0)0_2- then X can not contain an -0- group;
or a pharmaceutically acceptable salt thereof.

Unless otherwise specified, as used herein, the terms "alkyl", "alkenyl", "alkynyl"
and the prefix "alk-" are inclusive of both straight chain and branched chain groups and of cyclic groups, e.g., cycloalkyl and cycloalkenyl. Unless otherwise specified, these groups contain from 1 to 20 carbon atoms, with alkenyl groups containing from 2 to 20 carbon atoms, and alkynyl groups containing from 2 to 20 carbon atoms. In some embodiments, these groups have a total of up to 10 carbon atoms, up to 8 carbon atoms, up to 6 carbon atoms, or up to 4 carbon atoms. Cyclic groups can be monocyclic or polycyclic and preferably have from 3 to 10 ring carbon atoms. Exemplary cyclic groups include cyclopropyl, cyclobutyl, cyclopropylmethyl, cyclopentyl, cyclopentylmethyl, cyclohexyl, cyclohexylmethyl, adamantyl, and substituted and unsubstituted bornyl, norbomyl, and norbornenyl.
Unless otherwise specified, "alkylene", "alkenylene", and "alkynylene" are the divalent forms of the "allcyl", "alkenyl", and "alkynyl" groups defined above.
The terms, "alkylenyl", "alkenylenyl", and "alkynylenyl" are use when "alkylene", "alkenylene", and "alkynylene," respectively, are substituted. For example, an arylalkylenyl group comprises an alkylene moiety to which an aryl group is attached.
The term "haloalkyl" is inclusive of groups that are substituted by one or more halogen atoms, including perfluorinated groups. This is also true of other groups that include the prefix "halo-." Examples of suitable haloalkyl groups are chloromethyl, chlorobutyl, trifluoromethyl, 2,2,2-trifluoroethyl, and the like.
The term "aryl" as used herein includes carbocyclic aromatic rings or ring systems.
Examples of aryl groups include phenyl, naphthyl, biphenyl, fluorenyl and indenyl.
Unless otherwise indicated, the term "heteroatom" refers to the atoms 0, S, or N.
The term "heteroaryl" includes aromatic rings or ring systems that contain at least one ring heteroatom (e.g., 0, S, N). In some embodiments, the term "heteroaryl" includes a ring or ring system that contains 2-12 carbon atoms, 1-3 rings, 1-4 heteroatoms, and 0, S, and N as the heteroatoms. Exemplary heteroaryl groups include furyl, thienyl, pyridyl, quinolinyl, isoquinolinyl, indolyl, isoindolyl, triazolyl, pyrrolyl, tetrazolyl, imidazolyl, pyrazolyl, oxazolyl, thiazolyl, benzofuranyl, benzothiophenyl, carbazolyl, benzoxazolyl, pyrimidinyl, benzimidazolyl, quinoxalinyl, benzothiazolyl, naphthyridinyl, isoxazolyl, isothiazolyl, purinyl, quinazolinyl, pyrazinyl, 1-oxidopyridyl, pyridazinyl, triazinyl, tetrazinyl, oxadiazolyl, thiadiazolyl, and so on.
The term "heterocyclyl" includes non-aromatic rings or ring systems that contain at least one ring heteroatom (e.g., 0, S, N) and includes all of the fully saturated and partially unsaturated derivatives of the above mentioned heteroaryl groups. In some embodiments, the term "heterocyclyl" includes a ring or ring system that contains 2-12 carbon atoms, 1-3 rings, 1-4 heteroatoms, and 0, S, and N as the heteroatoms. Exemplary heterocyclyl groups include pyrrolidinyl, tetrahydrofuranyl, morpholinyl, thiomorpholinyl, 1,1-dioxothiomorpholinyl, piperidinyl, piperazinyl, thiazolidinyl, imidazolidinyl, isothiazolidinyl, tetrahydropyranyl, quinuclidinyl, homopiperidinyl (azepanyl), 1,4-oxazepanyl, homopiperazinyl (diazepanyl), 1,3-dioxolanyl, aziridinyl, azetidinyl, dihydroisoquinolin-( l IH)-yl, octahydroisoquinolin-(1 H)-yl, dihydroquinolin-(2H)-yl, octahydroquinolin-(2B)-yl, dihydro-lH-imidazolyl, 3-azabicyclo[3.2.2]non-3-yl, and the like.
The term "heterocyclyl" includes bicylic and tricyclic heterocyclic ring systems.
Such ring systems include fused and/or bridged rings and spiro rings. Fused rings can include, in addition to a saturated or partially saturated ring, an aromatic ring, for example, a benzene ring. Spiro rings include two rings joined by one spiro atom and three rings joined by two spiro atoms.
When "heterocyclyl" contains a nitrogen atom, the point of attachment of the heterocyclyl group may be the nitrogen atom.
The terms "arylene", "heteroarylene", and "heterocyclylene" are the divalent forms of the "aryl", "heteroaryl", and "heterocyclyl" groups defined above. The terms, "arylenyl", "heteroarylenyl", and "heterocyclylenyl" are used when "arylene", "heteroarylene", and "heterocyclylene", respectively, are substituted. For example, an alkylarylenyl group comprises an arylene moiety to which an alkyl group is attached.
When a group (or substituent or variable) is present more than once in any Formula described herein, each group (or substituent or variable) is independently selected, whether explicitly stated or not. For example, for the formula -N(R8)-C(O)-N(R8)- each R8 group is independently selected.
The invention is inclusive of the compounds described herein in any of their pharmaceutically acceptable forms, including isomers (e.g., diastereomers and enantiomers), salts, solvates, polymorphs, and the like. In particular, if a compound is optically active, the invention specifically includes each of the compound's enantiomers as well as racemic mixtures of the enantiomers. It should be understood that the term "compound" includes any or all of such forms, whether explicitly stated or not (although at times, "salts" are explicitly stated).
The term "prodrug" means a compound that can be transformed in vivo to yield an immune response modifying compound, including any of the salt, solvated, polymorphic, or isomeric forms described above. The prodrug, itself, may be an immune response modifying compound, including any of the salt, solvated, polymorphic, or isomeric forms described above. The transformation may occur by various mechanisms, such as through a chemical (e.g., solvolysis or hydrolysis, for example, in the blood) or enzymatic biotransformation. A discussion of the use of prodrugs is provided by T.
Higuchi and W.
Stella, "Pro-drugs as Novel Delivery Systems," Vol. 14 of the A. C. S.
Symposiunl Series, and in Bioreversible Carriers in Drug Design, ed. Edward B. Roche, American Pharmaceutical Association and Pergamon Press, 1987.
For any of the compounds presented herein, each one of the following variables (e.g., Y, X, RI, Q, Gi, Ga, n, and so on) in any of its embodiments can be combined with any one or more of the other variables in any of their embodiments and associated with any one of the fomiulas described herein, as would be understood by one of skill in the art.
Each of the resulting combinations of variables is an embodiment of the present invention.
For certain embodiments of Formula I, II, or III, n is 1.
For certain embodiments of Formula I, II, or III, n is 2.
For certain embodiments of Formula I, II, or III, including any one of the above embodiments, m is 0.
For certain embodiments of Formula I, II, or III, including any one of the above embodiments, Rt is -X-Y-R4 wherein X is straight chain or branched chain C1_6 alkylene which may be interrupted by one -0- group; Y is selected from the group consisting of -N(R8)-C(O)-, -N(R8)-S(O)2-, -N(R8)-C(O)-N(R8)-, and -S(O)2- wherein R8 is selected from hydrogen and methyl; and R4 is selected from the group consisting of C1_6 alkyl, isoquinolinyl, N-methylimidazolyl, pyridinyl, quinolinyl, phenyl, and phenyl substituted by a substituent selected from the group consisting of chloro, cyano, fluoro, hydroxy, and methyl; with the proviso that when Y is -S(0)2- then X can not contain an -0-group. For certain of these embodiments, as well as any one of the above embodiments, Rl is selected from the group consisting of 2-[(cyclopropylcarbonyl)amino]ethyl, 4-[(cyclopropylcarbonyl)amino]butyl, 2-[(cyclohexylcarbonyl)aminoj-2-methylpropyl, 2-{[(1-methylethyl)carbonyl]amino}ethyl, 4-{[(1-methylethyl)carbonyl]amino}butyl, 2-methyl-2-{[(1-methylethyl)carbonyl]amino}propyl, 2-[(methylsulfonyl)amino]ethyl, 4-[(methylsulfonyl)amino]butyl, 2-methyl-2-[(methylsulfonyl)amino]propyl, 2-methyl-2-({ [(1-methylethyl)amino]carbonyl} amino)propyl, and 2,2-dimethyl-3-(methylsulfonyl)propyl.
For certain embodiment, including any one of the above embodiments of Formulas I, II, and III, RI is -X-Y-R4 wherein X is straight chain or branched chain CI_$ alkylene which may be interrupted by one -0- group; Y is selected from the group consisting of -N(R8)-C(O)-, -N(R$)-S(O)2-, -N(R8)-S(O)2-N(R8a)-, -N(R8)-C(O)-N(R$a)-, and -S(O)2-wherein R8 is hydrogen, methyl, benzyl, or pyridin-3-ylmethyl; R8a is hydrogen, methyl, or ethyl, and R4 is selected from the group consisting of C1_7 alkyl, haloC1_4 alkyl, hydroxyC1_4 alkyl, phenyl, benzyl, 1-phenylethyl, 2-phenylethyl, 2-phenylethenyl, phenylcyclopropyl, pyridinyl, thienyl, N-methylimidazolyl, 3,5-dimethylisoxazolyl, wherein benzyl is unsubstituted or substituted by a methyl group, and phenyl is unsubstituted or substituted by one or two substituents independently selected from the group consisting of methyl, fluoro, chloro, cyano, hydroxy, and dimethylamino;
with the proviso that when Y is -S(0)2- then X can not contain an -0- group. For certain of these embodiments, Y is selected from the group consisting of -N(R$)-C(O)-, -N(R$)-S(O)Z-, and -N(R$)-C(O)-N(R8a)-. For certain of these embodiments, Rsa is hydrogen.
Alternatively, for certain of these embodiments, R8a is methyl. For certain of these embodiments, R8 is hydrogen. Alternatively, for certain of these embodiments, R8 is benzyl. Alternatively, for certain of these embodiments, R8 is pyridin-3-ylmethyl.
Alternatively, for certain of these embodiments, Y is -S(O)Z-. For certain of these embodiments, X is C1_6 alkylene.
For certain embodiments of Formula I, II, or III, including any one of the above embodiments except where Rl is -X-Y-R4, RI is -X-R5, wherein X is straight chain or - r(CHZ)a 1 N--S(O)2 -N(R$) -C(O)-N A
branched chain C1_6 alkylene, and R5 is C R' or \(CH2)b~ . For (-(CH2)a -~
-N(R$)-C(O)-N A
certain of these embodiments, R5 is \(CH2)b-I/ . For certain of these embodiments, R8 is hydrogen, methyl, or pyridin-3-ylmethyl, A is -0-, -CH2-, or -N(CH3)-, a is 1 or 2, and b is 2. For certain of these embodiments, Rl is selected from the group consisting of 4-(l,l-dioxidoisothiazolidin-2-yl)butyl, 4-[(4-morpholinecarbonyl)amino]butyl, and 2-[(4-morpholinecarbonyl)amino]ethyl.

For certain embodiments of Formula I, II, or III, including any one of the above embodiments except where Rl is -X-Y-R4 or -X-R5, Rl is -C1_4 alkylenyl-Het.
For certain of these embodiments, as well as any one of the above embodiments where Het is present, Het is selected from the group consisting of tetrahydropyranyl and tetrahydrofuranyl. For certain of these embodiments, as well as any one of the above embodiments where Het is present, R1 is tetrahydro-2H-pyran-4-ylmethyl.
For certain embodiments, for example, embodiments of Formula I, the present invention provides a compound selected from the group consisting of N-[4-(4-amino-2-hydroxymethyl-lH-imidazo[4,5-c]quinolin-l-yl)butyl]methanesulfonamide and N-{4-[4-amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c]quinolin-l-yl]butyl]}methanesulfonamide, or a pharmaceutically acceptable salt thereof.
For certain embodiments, for example, embodiments of Formula I, the present invention provides N- {2-[4-amino-2-(hydroxymethyl)-1 H-imidazo[4,5-c]quinolin-l-yl]-1,1-dimethylethyl.}methanesulfonamide or a pharmaceutically acceptable salt thereof.
For certain embodiments, the present invention provides a pharmaceutical composition comprising a therapeutically effective amount of a compound or salt of Formula I, II, III, or of any one of the above embodiments and a pharmaceutically acceptable carrier.
For certain embodiments, the present invention provides a method of preferentially inducing the biosynthesis of IFN-a in an animal comprising administering an effective amount of a compound or salt of Formula I, II, III, or of any one of the above embodiments or the above pharmaceutical composition to the animal.
For certain embodiments, the present invention provides a method of treating a viral disease in an animal in need thereof comprising administering a therapeutically effective amount of a compound or salt of Formula I, II, III, or of any one of the above embodiments or the above pharmaceutical composition to the animal.
For certain embodiments, the present invention provides a method of treating a neoplastic disease in an animal in need thereof comprising administering a therapeutically effective amount of a compound or salt of Formula I, II, III, or of any one of the above embodiments or the above pharmaceutical composition to the animal.
For certain embodiments of the above methods, the compound or salt or pharmaceutical coniposition is administered systemically.

For certain einbodiments, R is selected from the group consisting of C1_lo alkyl, C1.10 alkoxy, halogen, and C1_lo haloalkyl.
For certain embodiments, RI is selected from the group consisting of -X-Y-R4, -X-R5, and -X-Het.
For certain embodiments, Rl is -X-Y-R4.
For certain embodiments, Rl is -X-Y-R4 wherein X is straight chain or branched chain C1.6 allcylene which may be interrupted by one -0- group; Y is selected from the group consisting of -N(R8)-C(O)-, -N(R8)-S(O)2-, -N(R8)-C(O)-N(R$)-, and -S(O)2-wherein R$ is selected from hydrogen and methyl; and R4 is selected from the group consisting of C1.6 alkyl, isoquinolinyl, N-methylimidazolyl, pyridinyl, quinolinyl, phenyl, and phenyl substituted by a substituent selected from the group consisting of chloro, cyano, fluoro, hydroxy, and methyl.
For certain embodiments, RI is selected from the group consisting of 2-[(cyclopropylcarbonyl)amino]ethyl, 4-[(cyclopropylcarbonyl)amino]butyl, 2-[(cyclohexylcarbonyl)amino]-2-methylpropyl, 2- { [(1-methylethyl)carbonyl]amino}ethyl, 4-{ [(1-methylethyl)carbonyl]amino}butyl, 2-methyl-2-{ [(1-methylethyl)carbonyl]amino}propyl, 2-[(methylsulfonyl)amino]ethyl, 4-[(methylsulfonyl)amino]butyl, 2-methyl-2-[(methylsulfonyl)amino]propyl, 2-methyl-2-({ [(1-methylethyl)amino]carbonyl}amino)propyl, and 2,2-dimethyl-3 -(methylsulfonyl)propyl.
For certain embodiments, RI is -X-R5.
For certain embodiments, Rl is -X-R5 wherein X is straight chain or branched --v- S(O)2 r(CH2)a ~
-N(Rs) -C(O)-- \ A
chain C1_6 alkylene, and R5 is ( R' or (CH2)b-~/ .

For certain embodiments, Rl is selected from the group consisting of 4-(1,1-dioxidoisothiazolidin-2-yl)butyl, 4-[(4-morpholinecarbonyl)amino]butyl, and 2-[(4-morpholinecarbonyl)amino]ethyl.
For certain embodiments, Rl is -X-Het.
For certain embodiments, Rl is -C1_4 alkylenyl-Het.
For certain embodiments, Rl is tetrahydro-2H-pyran-4-ylmethyl.

For certain embodiments, R4 is selected from the group consisting of hydrogen, alkyl, alkenyl, aryl, arylalkylenyl, heteroaryl, and heteroarylalkylenyl, wherein the alkyl, alkenyl, aryl, arylalkylenyl, heteroaryl, and heteroarylalkylenyl, groups can be unsubstituted or substituted by one or more substituents independently selected from the group consisting of alkyl, alkoxy, hydroxyalkyl, haloalkyl, haloalkoxy, halogen, nitro, hydroxy, mercapto, cyano, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocyclyl, amino, alkylamino, dialkylamino, and in the case of alkyl and alkenyl, oxo.
For certain embodiments, R4 is selected from the group consisting of C1_6 allcyl, isoquinolinyl, N-methylimidazolyl, pyridinyl, quinolinyl, phenyl, and phenyl substituted by a substituent selected from the group consisting of chloro, cyano, fluoro, hydroxy, and methyl.
For certain embodiments, R4 is selected from the group consisting of C1 _7 alkyl, haloC14 alkyl, hydroxyCl4 alkyl, phenyl, benzyl, 1-phenylethyl, 2-phenylethyl, phenylethenyl, phenylcyclopropyl, pyridinyl, thienyl, N-methylimidazolyl, 3,5-dimethylisoxazolyl, wherein benzyl is unsubstituted or substituted by a methyl group, and phenyl is unsubstituted or substituted by one or two substituents independently selected from the group consisting of methyl, fluoro, chloro, cyano, hydroxy, and dimethylamino.
For certain embodiments, R4 is C1_7 alkyl.
For certain embodiments, R4 is CI4 alkyl.
For certain embodiments, R4 is phenyl which is unsubstituted or substituted by one or two substituents independently selected from the group consisting of methyl, fluoro, chloro, cyano, hydroxy, and dimethylamino.
For certain embodiments, R5 is selected from the group consisting of ~ (CHZ)a -N- C(R6) -N- S(O)Z - N(R$)-C(R6)-N A
R~~ R7/ ~-(CHz)b_/
, , and r(CH2)a -'~
N-C(R6)-N A
R~o J (CHz)b r(CHz)a -N- S(O)Z -N(R$) -C(O)-N A
For certain embodiments, R5 is R7 or (CH2)b r(CHZ)a '~
-N(R8)-C(O)-N A
For certain embodiments, R5 is \(CH2)b-I/ .
For certain embodiments, R6 is selected from the group consisting of =O and =S.
For certain embodiments, R6 is =0.
For certain embodiments, R6 is S.
For certain embodiments, R7 is Ca.7 alkylene.
For certain embodiments, R7 is C2-4 alkylene.
For certain embodiments, R$ is selected from the group consisting of hydrogen, alkyl, alkoxyalkylenyl, hydroxyalkylenyl, arylalkylenyl, and heteroarylalkylenyl.
For certain embodiments, R8 is selected from the group consisting of hydrogen, C1_4 alkyl, and Ci-4 alkoxyC1.4 alkylenyl.
For certain embodiments, R8 is arylalkylenyl.
For certain embodiments, R$ is benzyl.
For certain embodiments, R8 is heteroarylalkylenyl.
For certain embodiments, R8 is pyridin-3-ylmethyl.
For certain embodiments, R8 is hydrogen or C1-4 alkyl.
For certain embodiments, R8 is selected from hydrogen and methyl For certain embodiments, R8 is hydrogen.
For certain embodiments, Rlo is C3.$ alkylene.
For certain embodiments, Rlo is C4.6 alkylene.
For certain embodiments, A is selected from the group consisting of -0-, -C(O)-, -CH2-, -S(O)o-a-, and -N(Q-R4)-.
For certain embodiments, A is -0-, -CH2-, or -N(Q-R4)-.
For certain embodiments, A is -0-, -CH2-, -S-, or -S(0)2-.
For certain embodiments, A is -0- or -S(O)2-.
For certain embodiments, A is -0-.
For certain embodiments, A is -CH2-.
For certain embodiments, A is -N(Q-R4)-.
For certain embodiments, A is -N(CH3)-.

For certain embodiments, including any one of the above embodiments of Formula II, Gl is selected from the group consisting of -C(O)-R', a-aminoacyl, a-aminoacyl-a-aminoacyl, -C(O)-O-R', -C(O)-N(R")R', -C(=NY')-R', -CH(OH)-C(O)-OY', -CH(OC1_4 alkyl)Yo, -CH2Y1 , and -CH(CH3)Y1; R and R" are independently selected from the group consisting of Ct_lo allcyl, C3.7 cycloallcyl, phenyl, benzyl, and 2-phenylethyl, each of which may be unsubstituted or substituted by one or more substituents independently selected from the group consisting of halogen, hydroxy, nitro, cyano, carboxy, C1_6 alkyl, C1_4 alkoxy, aryl, heteroaryl, aryl-C1.4 alkylenyl, heteroaryl-C1_4 alkylenyl, halo-C1.4 alkylenyl, halo-C1_a alkoxy, -O-C(O)-CH3, -C(O)-O-CH3, -C(O)-NH2, -O-CHZ-C(O)-NH2, -NH2, and -S(O)a-NHa, with the proviso that R" can also be hydrogen;
a-aminoacyl is an a-aminoacyl group derived from an a-amino acid selected from the group consisting of racemic, D-, and L-amino acids; Y' is selected from the group consisting of hydrogen, C1_6 alkyl, and benzyl; Yo is selected from the group consisting of C1_6 alkyl, carboxy-C1.6 alkylenyl, amino-C1_4 alkylenyl, mono-N-C1_& alkylamino-C1.4 alkylenyl, and di-NNV C1_6 alkylamino-Ci-4 alkylenyl; and Yl is selected from the group consisting of mono-lV-C1.6 alkylamino, di-N,1V C1.6 alkylamino, morpholin-4-yl, piperidin-1-yl, pyrrolidin-1-yl, and 4-C1_4 alkylpiperazin-1-yl.
For certain embodiments, including any one of the above embodiments of Formula II, Gl is selected from the group consisting of -C(O)-R', a-aminoacyl, and -C(O)-O-R'.
For certain of these embodiments, R' contains one to ten carbon atoms. For certain of these embodiments, a-aminoacyl is an a-C2_11 aminoacyl group derived from an a-amino acid selected from the group consisting of racemic, D-, and L-amino acids containing a total of at least 2 carbon atoms and a total of up to 11 carbon atoms, and may also include one or more heteroatoms selected from the group consisting of 0, S, and N.
For certain embodiments, including any one of the above embodiments of Formula III, G2 is selected from the group consisting of -X2-C(O)-R', a-aminoacyl, a-aminoacyl-a-aminoacyl, -XZ-C(O)-O-R', and -C(O)-N(R")R'. For certain of these embodiments, X2 is selected from the group consisting of a bond; -CH2-0-; -CH(CH3)-O-; -C(CH3)2-0-; and, in the case of -X2-C(O)-O-R', -CH2-NH-; R' and R" are independently selected from the group consisting of C1_1Q alkyl, C3_7 cycloalkyl, phenyl, benzyl, and 2-phenylethyl, each of which may be unsubstituted or substituted by one or more substituents independently selected from the group consisting of halogen, hydroxy, nitro, cyano, carboxy, Cl_6 alkyl, C 1_4 alkoxy, aryl, heteroaryl, aryl-C 1_4 alkylenyl, heteroaryl-C 14 alkylenyl, halo-Ci_4 alkylenyl, halo-C1_4 alkoxy, -O-C(O)-CH3, -C(O)-O-CH3, -C(O)-NH2, -O-CH2-C(O)-NH2, -NH2, and -S(O)2-NH2, with the proviso that R" can also be hydrogen;
and a-aminoacyl is an a-aminoacyl group derived from an a-amino acid selected from the group consisting of racemic, D-, and L-amino acids.
For certain embodiments, including any one of the above embodiments of Formula III, GZ is selected from the group consisting of -C(O)-R' and a-aminoacyl, wherein R' is C1_6 alkyl or phenyl which is unsubstituted or substituted by one or more substituents independently selected from the group consisting of halogen, hydroxy, nitro, cyano, carboxy, C1_6 alkyl, C1_4 alkoxy, aryl, heteroaryl, aryl-C1_4 alkylenyl, heteroaryl-C1_4 alkylenyl, halo-C1_4 alkylenyl, halo-C1_4 alkoxy, -O-C(O)-CH3, -C(O)-O-CH3, -C(O)-NH2, -O-CHa-C(O)-NH2, -NH2, and -S(O)2-NH2.
For certain embodiments, including any one of the above embodiments of Formula III, G2 is selected from the group consisting of a-amino-CZ_5 alkanoyl, C2_6 alkanoyl, C1_6 alkoxycarbonyl, and CI_6 alkylcarbamoyl.
For certain embodiments, including any one of the above embodiments which include an a-aminoacyl group, a-aminoacyl is an a-aminoacyl group derived from a naturally occuring a-amino acid selected from the group consisting of racemic, D-, and L-amino acids.
For certain embodiments, including any one of the above embodiments which include an a-aminoacyl group, a-aminoacyl is an a-aminoacyl group derived from an a-amino acid found in proteins, wherein the the amino acid is selected from the group consisting of racemic, D-, and L-amino acids.
For certain embodiments, the hydrogen atom of the hydroxy group of Formula II
(including any one of its embodiments) is replaced by G2, wherein G2 is defined as in any one of the above embodiments of G2.
For certain embodiments, Het is selected from the group consisting of tetrahydropyranyl and tetrahydrofuranyl.
For certain embodiments, Het is tetrahydro-2H-pyran-4-yl.
For certain embodiments, Q is selected from the group consisting of a bond, -C(R6)-, -S(O)2, -C(R6)-N(R8)-, -S(O)2-N(R8)-, -C(R6)-O-, and -C(R6)-S-.

For certain embodiments, Q is selected from the group consisting of a bond, -C(R6)-, -S(O)a-, and -C(R6)-N(R8)-.
For certain embodiments, Q is selected from the group consisting of -C(O)-, -S(O)a-, and -C(O)-N(R8)-. In certain of these embodiments, Rs is hydrogen or methyl, For certain embodiments, Q is -C(O)-.
For certain embodiments, Q is -S(O)2-.
For certain embodiments, Q is -C(R6)-N(R8)-.
For certain embodiments, Q is -C(O)-N(Rs)- wherein Rs is hydrogen or methyl.
For certain embodiments, X is straight chain or branched chain alkylene optionally interrupted by one -0- group.
For certain embodiments, X is straight chain or branched chain C1_6 alkylene which may be interrupted by one -0- group.
For certain einbodiments, X is straight chain or branched chain Cl_$ alkylene.
For certain embodiments, X is straight chain or branched chain C1_6 alkylene.
For certain embodiments, X is straight chain or branched chain C1_4 alkylene.
For certain embodiments, X is ethylene.
For certain embodiments, X is propylene.
For certain embodiments, X is butylene.
For certain embodiments, X is -CH2-C(CH3)2-.
For certain embodiments, Y is selected from the group consisting of -S(O)9_2-and -N(R8)-Q-, with the proviso that when Y is -S(O)o_z- then X does not contain an -0- group.
For certain embodiments, Y is selected from the group consisting of -N(Rs)-C(O)-, -N(Rs)-S(0)2-, -N(Rs)-C(O)-N(Rs)-, and -S(0)2-, with the proviso that when Y
is -S(0)2-then X does not contain an -0- group. In certain of these embodiments, Rs is selected from hydrogen and methyl.
For certain embodiments, Y is selected from the group consisting of -N(R8)-C(O)-, N(Rs)-S(O)Z-, N(Rs)-S(O)z-N(Rsa)-, -N(Rg)-C(O)-N(Rsa)-, and -S(0)2-.
For certain embodiments, Y is selected from the group consisting of -N(Rs)-C(O)-, -N(Rs)-S(O)2-, -N(Rs)-C(O)-N(Rsa)-.
For certain embodiments, Y is -S(0)2-.
For certain embodiments, a and b are independently integers from 1 to 6 with the proviso that a+ b is < 7.

For certain embodiments, a and b are each independently 1 to 3.
For certain embodiments, a and b are each 2.
For certain embodiments, a is 1, 2, or 3, and b is 2.
For certain embodiments, a is 1 or 2, and b is 2.
For n certain embodiments, n is 1 or 2.
For certain embodiments, n is 1.
For certain embodiments, n is 2.
For certain embodiments, m is 0 or 1.
For certain embodiments, in is 0.
For certain embodiments, m is 1.
Preparation of the Compounds Compounds of the invention may be synthesized by synthetic routes that include processes analogous to those well known in the chemical arts, particularly in light of the description contained herein. The starting materials are generally available from commercial sources such as Aldrich Chemicals (Milwaukee, Wisconsin, USA) or are readily prepared using methods well known to those skilled in the art (e.g., prepared by methods generally described in Louis F. Fieser and Mary Fieser, Reagents for Organic Synthesis, v. 1-19, Wiley, New York, (1967-1999 ed.); Alan R. Katritsky, Otto Meth-Cohn, Charles W. Rees, Comprehensive Organic Functional Group Transformations, v. 1-6, Pergamon Press, Oxford, England, (1995); Barry M. Trost and Ian Fleming, Comprehensive Organic Syntlzesis, v. 1-8, Pergamon Press, Oxford, England, (1991); or Beilsteins Handbuch der organischen Chemie, 4, Aufl. Ed. Springer-Verlag, Berlin, Germany, including supplements (also available via the Beilstein online database)).
For illustrative purposes, the reaction schemes depicted below provide potential routes for synthesizing the compounds of the present invention as well as key intermediates. For more detailed description of the individual reaction steps, see the EXAMPLES section below. Those skilled in the art will appreciate that other synthetic routes may be used to synthesize the compounds of the invention. Although specific starting materials and reagents are depicted in the reaction schemes and discussed below, other starting materials and reagents can be easily substituted to provide a variety of derivatives and/or reaction conditions. In addition, many of the compounds prepared by the methods described below can be further modified in light of this disclosure using conventional methods well known to those skilled in the art.
In the preparation of compounds of the invention it may sometimes be necessary to protect a particular functionality while reacting other functional groups on an intermediate.
The need for such protection will vary depending on the nature of the particular functional group and the conditions of the reaction step. Suitable amino protecting groups include acetyl, trifluoroacetyl, tert-butoxycarbonyl (Boc), benzyloxycarbonyl, and 9-fluorenylmethoxycarbonyl (Fmoc). Suitable hydroxy protecting groups include acetyl and silyl groups such as the tert-butyl dimethylsilyl group. For a general description of protecting groups and their use, see T. W. Greene and P. G. M. Wuts, Protective Groups in Organic Synthesis, John Wiley & Sons, New York, USA, 1991.
Conventional methods and techniques of separation and purification can be used to isolate compounds of the invention, as well as various intermediates related thereto. Such techniques may include, for example, all types of chromatography (high performance liquid chromatography (HPLC), column chromatography using common absorbents such as silica gel, and thin layer chromatography), recrystallization, and differential (i.e., liquid-liquid) extraction techniques.
In some embodiments, compounds of the invention can be prepared according to Reaction Scheme I, wherein Rl, R, m, and n are as defined above and alkyl is methyl or ethyl.
In Reaction Scheme I an ether substituted 1H-imidazo[4,5-c]quinolin-4-amine of Formula X is cleaved to provide a hydroxyalkyl substituted 1H-imidazo[4,5-c]quinolin-4-amine of Formula I. The reaction is conveniently carried out by adding a solution of boron tribromide in a suitable solvent such as dichloromethane to a solution or suspension of a compound of Formula X in a suitable solvent such as dichloromethane at ambient or at a sub-ambient temperature, for example, at 0 C. The product or pharmaceutically acceptable salt thereof can be isolated using conventional methods.
Numerous compounds of Formula X are known; others can be prepared using known synthetic methods. See, for example, United States Patent Nos.
6,069,149;
6,331,539; 6,451,810; 6,541,485; 6,756,382; 6,677,349; 6,573,273; 6,664,264;
6,664,265;
6,677,347; 6,660,735; 6,683,088; and 6,667,312 and the references cited therein.

Reaction Scheme I

~j / ~~--(CHz)n'O-alkyf ---n N N~(CH2),OH
N
~ '~ R, k (R)m X (R)m In some embodiments, compounds of the invention can be prepared according to Reaction Scheme II, wherein Rl, Gl, and n are as defined above. Compounds of Formula I can be prepared according to the method described above. The amino group of a compound of Formula I can be converted by conventional methods to a functional group such as an amide, carbamate, urea, amidine, or another hydrolyzable group. A
compound of this type can be made by the replacement of a hydrogen atom in an amino group with a group such as -C(O)-R', a-aminoacyl, a-aminoacyl-a-aminoacyl, -C(O)-O-R', -C(O)-N(R")R, -C(--NY')-R', -CH(OH)-C(O)-OY', -CH(OC1-4 alkyl)Yo, -CH2Y1, and -CH(CH3)Yl; wherein R' and R" are independently selected from the group consisting of C1_io alkyl, C3_7 cycloalkyl, phenyl, benzyl, and 2-phenylethyl, each of which may be unsubstituted or substituted by one or more substituents independently selected from the group consisting of halogen, hydroxy, nitro, cyano, carboxy, C1_6 alkyl, Cl.4 alkoxy, aryl, heteroaryl, aryl-C1_4 alkylenyl, heteroaryl-C14 alkylenyl, halo-Cl_4 alkylenyl, halo-CI_4 alkoxy, -O-C(O)-CH3, -C(O)-O-CH3, -C(O)-NH2, -O-CH2-C(O)-NHa, -NH2, and -S(O)Z-NHZ, with the proviso that R" can also be hydrogen; each a-aminoacyl is an a-aminoacyl group derived from an a-amino acid selected from the group consisting of racemic, D-, and L-amino acids; Y' is selected from the group consisting of hydrogen, C1_6 alkyl, and benzyl; Yo is selected from the group consisting of C1_6 alkyl, carboxy-C1_6 alkylenyl, amino-C1.4 alkylenyl, mono-N-C1_6 alkylamino-C1_4 alkylenyl, and di-N,N-C1_6 alkylamino-CI_~ alkylenyl; and Yl is selected from the group consisting of mono-N-C1_6 alkylamino, di-N,N-C1_6 alkylamino, morpholin-4-yl, piperidin-l-yl, pyrrolidin-1-yl, and 4-C1_4 alkylpiperazin-l-yl. Particularly useful compounds of Formula II are amides derived from carboxylic acids containing one to ten carbon atoms, amides derived from amino acids, and carbamates containing one to ten carbon atoms.
The reaction can be carried out, for example, by combining a compound of Formula I
with a chloroformate or acid chloride, such as ethyl chloroformate or acetyl chloride, in the presence of a base such as triethylamine in a suitable solvent such as dichloromethane at ambient temperature.
Alternatively, the hydroxy group on a compound of Formula I can be protected using a suitable silyl group such as tert-butyl dimethylsilyl using conventional methods.
The Gi group may then be installed using conventional methods followed by the removal of the hydroxy protecting group under acidic conditions to provide a compound of Formula II.

Reaction Scheme II
G~

N N N
~}--(CHZ),OH ---~ N \>-(CHZ),,OH
N
N
Ri Ri (R)m (R)m I II
In some embodiments, compounds of the invention can be prepared according to Reaction Scheme III, wherein RI, G2, and n are as defined above. Compounds of Formula I can be prepared according to the method described above. The hydrogen atom of the alcohol group of a compound of Formula I can be replaced using conventional methods with a group such as X2-C(O)-R', a-aminoacyl, a-aminoacyl-a-aminoacyl, -X2-C(O)-O-R', and -C(O)-N(R")R'; wherein X2 is selected from the group consisting of a bond;
-CHa-O-;
-CH(CH3)-0-; -C(CH3)2-O-; and, in the case of -Xa-C(O)-O-R', -CH2-NH-; R' and R" are independently selected from the group consisting of C1_10 alkyl, C3_7 cycloalkyl, phenyl, benzyl, and 2-phenylethyl, each of which may be unsubstituted or substituted by one or more substituents independently selected from the group consisting of halogen, hydroxy, nitro, cyano, carboxy, C1_6 alkyl, C14 alkoxy, aryl, heteroaryl, aryl-C1_4 alkylenyl, heteroaryl-C1_4 alkylenyl, halo-Ci_4 alkylenyl, halo-C1_4 alkoxy, -O-C(O)-CH3, -C(O)-O-CH3, -C(O)-NH2, -O-CH2-C(O)-NHZ, -NH2, and -S(O)2-NH2, with the proviso that R" can also be hydrogen; and each a-aminoacyl is an a-aminoacyl group derived from an a-amino acid selected from the group consisting of racemic, D-, and L-amino acids.
Particularly useful compounds of Formula III are esters made from carboxylic acids containing one to six carbon atoms, unsubstituted or substituted benzoic acid esters, or esters made from naturally occurring amino acids. For example, the reaction can be carried out by treating a compound of Formula I with a carboxylic acid or amino acid under Mitsunobu reaction conditions by adding triphenylphosphine and a carboxylic acid to a solution or suspension of a compound of Formula I in a suitable solvent such as tetrahydrofuran and then slowly adding diisopropyl azodicarboxylate. The reaction can be run at a sub-ambient temperature such as 0 C.
Reaction Scheme III

N N
N N~-(CHz)r,OH --~ N N~--(CH2)õO -G2 Ri Ri (R)m I (R>m III

In some embodiments, compounds of the invention can also be prepared using the synthetic methods described in the EXAMPLES below.

Pharmaceutical Compositions and Biological Activity Pharmaceutical compositions of the invention contain a therapeutically effective amount of a compound or salt described above in combination with a pharmaceutically acceptable carrier.
The terms "a therapeutically effective amount" and "effective amount" mean an amount of the compound or salt sufficient to induce a therapeutic or prophylactic effect, such as cytokine induction, immunomodulation, antitumor activity, and/or antiviral activity. Cytokine induction can include preferentially inducing the biosynthesis of IFN-a.
The exact amount of compound or salt used in a pharmaceutical composition of the invention will vary according to factors known to those of skill in the art, such as the physical and chemical nature of the compound or salt, the nature of the carrier, and the intended dosing regimen.
In some embodiments, the compositions of the invention will contain sufficient active ingredient or prodrug to provide a dose of about 100 nanograms per kilogram (ng/kg) to about 50 milligrams per kilogram (mg/kg), preferably about 10 micrograms per kilogram ( g/kg) to about 5 mg/kg, of the compound or salt to the subject.

In other embodiments, the compositions of the invention will contain sufficient active ingredient or prodrug to provide a dose of, for example, from about 0.01 mg/ma to about 5.0 mg/m2, computed according to the Dubois method, in which the body surface area of a subject (m) is computed using the subject's body weight: m2 = (wt kg '425 x height cm '725) x 0.007184, although in some embodiments the methods may be performed by administering a compound or salt or composition in a dose outside this range. In some of these embodiments, the method includes administering sufficient compound to provide a dose of from about 0.1 mglmz to about 2.0 mg/ m2 to the subject, for exaniple, a dose of from about 0.4 mg/ma to about 1.2 mg/m2.
A variety of dosage forms may be used, such as tablets, lozenges, capsules, parenteral formulations (e.g., intravenous formulations), syrups, creams, ointments, aerosol formulations, transdermal patches, transmucosal patches and the like.
These dosage forms can be prepared with conventional pharmaceutically acceptable carriers and additives using conventional methods, which generally include the step of bringing the active ingredient into association with the carrier.
The compounds or salts of the invention can be administered as the single therapeutic agent in the treatment regimen, or the compounds or salts described herein may be administered in combination with one another or with other active agents, including additional immune response modifiers, antivirals, antibiotics, antibodies, proteins, peptides, oligonucleotides, etc.
Compounds or salts of the invention have been shown to induce the production of certain cytokines in experiments performed according to the tests set forth below. These results indicate that the compounds or salts are useful for modulating the immune response in a number of different ways, rendering them useful in the treatment of a variety of disorders. The compounds or salts of the invention are especially useful as immune response modifiers due to their ability to preferentially induce interferon-a, thus providing a benefit over compounds that also induce pro-inflammatory cytokines (e.g.
TNF-a) or that induce pro-inflammatory cytokines at higher levels. While interferon-a and pro-inflammatory cytokines are beneficial in treating certain conditions, interferon-a preferentially induced is believed to be better tolerated by patients, because the significantly lower levels of pro-inflammatory cytokines can result in fewer or less severe adverse side effects experienced by patients. For example, if a subject is treated for a disease (e.g., hepatitis C, metastatic cancer) with a compound that induces significant levels of pro-inflammatory cytokines, while treating the disease, the compound may also cause side effects, such as severe and/or widespread inflammation, tissue destruction, or emesis, that render the subject unable or unwilling to receive the treatinent.
Alternatively, if a subject is treated with a compound that preferentially induces interferon-a then the compound may treat the disease with less risk of adverse side effects from pro-inflainmatory cytolcines such as TNF-a. Therefore, by maintaining the ability to treat a condition and reducing adverse side effects, compounds that preferentially induce IFN-a provide an advantage over compounds that would also induce pro-inflammatory cytokines, such as TNF-a, at higher levels.
The ability of the compounds or salts of the invention to preferentially induce the biosynthesis of IFN-a may be particularly advantageous when administered systemically, since adverse side effects, including for example widespread inflammation, may be reduced or even eliminated. Compounds of the invention may be administered systemically in a number of ways, including but not limited to oral and intravenous administration.
Cytokines whose biosynthesis may be induced by compounds or salts of the invention include IFN-a, IP-10, MCP-1, and a variety of other cytokines. In some instances, cytokines such as TNF-a, IL- 12 may be induced, albeit at significantly reduced levels. Among other effects, these and other cytokines can inhibit virus production and tumor cell growth, making the compounds or salts useful in the treatment of viral diseases and neoplastic diseases. Accordingly, the invention provides a method of inducing cytokine biosynthesis in an animal comprising administering an effective amount of a compound or salt of the invention to the animal. The animal to which the compound or salt is administered for induction of cytokine biosynthesis may have a disease as described infra, for example a viral disease or a neoplastic disease, and administration of the compound or salt may provide therapeutic treatment. Alternatively, the compound or salt may be administered to the animal prior to the animal acquiring the disease so that administration of the compound or salt may provide a prophylactic treatment.
In addition to the ability to induce the production of cytokines, compounds or salts of the invention can affect other aspects of the innate immune response. For example, the compounds or salts may cause maturation of dendritic cells or proliferation and differentiation of B-lymphocytes.
Wbether for prophylaxis or therapeutic treatment of a disease, and whether for effecting innate or acquired immunity, the compound or salt or composition may be administered alone or in combination with one or more active components as in, for example, a vaccine adjuvant. When adininistered with other components, the compound or salt or composition and otlier component or components may be administered separately; together but independently such as in a solution; or together and associated with one another such as (a) covalently linked or (b) non-covalently associated, e.g., in a colloidal suspension.
Conditions for which compounds or salts or compositions identified herein may be used as treatments include, but are not limited to:
(a) viral diseases such as, for example, diseases resulting from infection by an adenovirus, a herpesvirus (e.g., HSV-1, HSV-II, CMV, or VZV), a poxvirus (e.g., an orthopoxvirus such as variola or vaccinia, or molluscum contagiosum), a picornavirus (e.g., rhinovirus or enterovirus), an orthomyxovirus (e.g., influenzavirus), a paramyxovirus (e.g., parainfluenzavirus, niumps virus, measles virus, and respiratory syncytial virus (RSV)), a coronavirus (e.g., SARS), a papovavirus (e.g., papillomaviruses, such as those that cause genital warts, common warts, or plantar warts), a hepadnavirus (e.g., hepatitis B
virus), a flavivirus (e.g., hepatitis C virus or Dengue virus), or a retrovirus (e.g., a lentivirus such as HIV);
(b) bacterial diseases such as, for example, diseases resulting from infection by bacteria of, for example, the genus Escherichia, Enterobacter, Salmonella, Staphylococcus, Shigella, Listeria, Aerobacter, Helicobacter, Klebsiella, Proteus, Pseudomonas, Streptococcus, Chlamydia, Mycoplasma, Pneumococcus, Neisseria, Clostridium, Bacillus, Corynebacterium, Mycobacterium, Campylobacter, Vibrio, Serratia, Providencia, Chromobacterium, Brucella, Yersinia, Haemophilus, or Bordetella;
(c) other infectious diseases, such as chlamydia, fungal diseases including but not limited to candidiasis, aspergillosis, histoplasmosis, cryptococcal meningitis, or parasitic diseases including but not limited to malaria, pneumocystis carnii pneumonia, leishmaniasis, cryptosporidiosis, toxoplasmosis, and trypanosome infection;

(d) neoplastic diseases, such as intraepithelial neoplasias, cervical dysplasia, actinic keratosis, basal cell carcinoma, squamous cell carcinoma, renal cell carcinoma, Kaposi's sarcoma, melanoma, leukemias including but not limited to acute myeloid leukemia, acute lymphocytic leukemia, chronic myeloid leukemia, chronic lymphocytic leulcemia, multiple myeloma, Hodgkin's lymphoma, non-Hodgkin's lymphoma, cutaneous T-cell lymphoma, B-cell lymphoma, and hairy cell leulcemia, and other cancers;
(e) TH2-mediated, atopic diseases, such as atopic dermatitis or eczema, eosinophilia, asthma, allergy, allergic rhinitis, and Ommen's syndrome;
(f) certain autoimmune diseases such as systemic lupus erythematosus, essential thrombocythaemia, multiple sclerosis, discoid lupus, alopecia areata; and (g) diseases associated with wound repair such as, for example, inhibition of keloid formation and other types of scarring (e.g., enhancing wound healing, including chronic wounds).
Additionally, a compound or salt identified herein may be useful as a vaccine adjuvant for use in conjunction with any material that raises either humoral and/or cell mediated immune response, such as, for example, live viral, bacterial, or parasitic immunogens; inactivated viral, tumor-derived, protozoal, organism-derived, fungal, or bacterial immunogens; toxoids; toxins; self-antigens; polysaccharides;
proteins;
glycoproteins; peptides; cellular vaccines; DNA vaccines; autologous vaccines;
recombinant proteins; and the like, for use in connection with, for example, BCG, cholera, plague, typhoid, hepatitis A, hepatitis B, hepatitis C, influenza A, influenza B, parainfluenza, polio, rabies, measles, mumps, rubella, yellow fever, tetanus, diphtheria, hemophilus influenza b, tuberculosis, meningococcal and pneumococcal vaccines, adenovirus, HIV, chicken pox, cytomegalovirus, dengue, feline leukemia, fowl plague, HSV-1 and HSV-2, hog cholera, Japanese encephalitis, respiratory syncytial virus, rotavirus, papilloma virus, yellow fever, and Alzheimer's Disease.
Compounds or salts identified herein may be particularly helpful in individuals having compromised immune function. For example, compounds or salts may be used for treating the opportunistic infections and tumors that occur after suppression of cell mediated immunity in, for example, transplant patients, cancer patients and HIV patients.
Thus, one or more of the above diseases or types of diseases, for example, a viral disease or a neoplastic disease may be treated in an animal in need thereof (having the disease) by administering a therapeutically effective amount of a compound or salt of the invention to the animal.
An animal may also be vaccinated by administering an effective amount of a compound or salt described herein, as a vaccine adjuvant. In one embodiment, there is provided a method of vaccinating an animal comprising administering an effective amount of a compound or salt described herein to the animal as a vaccine adjuvant.
An amount of a compound or salt effective to induce cytokine biosynthesis is an amount sufficient to cause one or more cell types, such as dendritic cells and B-cells to produce an amount of one or more cytokines such as, for example, IFN-a, IP-10, and MCP-1 that is increased (induced) over a background level of such cytokines.
The precise amount will vary according to factors known in the art but is expected to be a dose of about 100 ng/kg to about 50 mg/kg, preferably about 10 g/kg to about 5 mg/kg.
In other embodiments, the amount is expected to be a dose of, for example, from about 0.01 mg/m2 to about 5.0 mg/ma, (computed according to the Dubois method as described above) although in sonle embodiments the induction of cytokine biosynthesis may be performed by administering a compound or salt in a dose outside this range. In some of these embodiments, the method includes administering sufficient compound or salt or composition to provide a dose of from about 0.1 mg/m2 to about 2.0 mg/ m2 to the subject, for example, a dose of from about 0.4 mg/m2 to about 1.2 mg/m''.
The invention provides a method of treating a disease which is responsive to the induction of cytokine biosynthesis, particularly the preferential induction of IFN-a, including a method of treating a viral infection in an animal and a method of treating a neoplastic disease in an animal, comprising administering an effective amount of a compound or salt or composition of the invention to the animal. An amount effective to treat or inhibit a viral infection is an amount that will cause a reduction in one or more of the manifestations of viral infection, such as viral lesions, viral load, rate of virus production, and mortality as compared to untreated control animals. The precise amount that is effective for such treatment will vary according to factors known in the art but is expected to be a dose of about 100 ng/kg to about 50 mg/kg, preferably about 10 g/kg to about 5 mg/kg. An amount of a compound or salt effective to treat a neoplastic condition is an amount that will cause a reduction in tumor size or in the number of tumor foci.
Again, the precise amount will vary according to factors known in the art but is expected to be a dose of about 100 ng/kg to about 50 mg/kg, preferably about 10 g/kg to about 5 mg/lcg. In other embodiments, the amount is expected to be a dose of, for example, from about 0.01 mg/m2 to about 5.0 mg/m2, (computed according to the Dubois method as described above) although in some embodiments either of these methods may be performed by administering a compound or salt in a dose outside this range. In some of these embodiments, the method includes administering sufficient compound or salt to provide a dose of from about 0.1 mg/ma to about 2.0 mg/ m2 to the subject, for example, a dose of from about 0.4 mg/m2 to about 1.2 mg/m2.
In addition to the formulations and uses described specifically herein, other formulations, uses, and administration devices suitable for compounds of the present invention are described in, for example, International Publication Nos. WO
03/077944 and WO 02/036592, U.S. Patent No. 6,245,776, and U.S. Publication Nos.
2003/0139364, 2003/185835, 2004/0258698, 2004/0265351, 2004/076633, and 2005/0009858.

Objects and advantages of this invention are further illustrated by the following examples, but the particular materials and amounts thereof recited in these examples, as well as other conditions and details, should not be construed to unduly limit this invention.

EXAMPLES
In the examples below normal high performance flash chromatography (prep HPLC) was carried out using a COMBIFLASH system (an automated high-performance flash purification product available from Teledyne Isco, Inc., Lincoln, Nebraska, USA) or a HORIZON HPFC system (an automated high-performance flash purification product available from Biotage, Inc, Charlottesville, Virginia, USA). The eluent used for each purification is given in the example. In some chromatographic separations, the solvent mixture 80/18/2 v/v/v chloroform/methanolfconcentrated ammonium hydroxide (CMA) was used as the polar component of the eluent. In these separations, CMA was mixed with chloroform in the indicated ratio.

Example 1 N-{3-[4-Amino-2-(2-hydroxyethyl)-1 H-imidazo[4,5-c]quinolin-1-yl]propyl } -4-methylbenzenesulfonamide NHZ
N N, ~ OH
N~>--J
'-~ N
'O
~S' O' Boron tribromide (5.50 mL of 1 M in dichloromethane) was added dropwise to a chilled (0 C) suspension of N-{3-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-l-yl]propyl}-4-methylbenzenesulfonamide (1.0 g, 2.2 mmol; U.S.
Patent No.
6,677,349, Example 253) in dichloromethane (20 mL). The reaction mixture was stirred at 0 C for 3 hours. The reaction mixture was quenched with methanol. Hydrochloric acid (about 10 mL of 6 N) was added and the mixture was stirred at 50 C overnight.
The mixture was diluted with water (50 mL) and ethyl acetate (100 mL) and then brought to neutral pH with solid sodium hydroxide. The layers were separated and the aqueous was extracted with ethyl acetate (x2). The combined organics were dried over magnesium sulfate, filtered, and then concentrated under reduced pressure to provide a yellow solid.
This material was purified by prep HPLC (COMBIFLASH system eluting first with a gradient of 0 to 5% methanol in dichloromethane containing 1% ammonium hydroxide and then with a gradient of 5 to 10% methanol in dichloromethane containing 1 %
ammonium hydroxide) to provide a white solid. This material was suspended in hot acetonitrile, allowed to cool, and then the solvent was decanted. The resulting material was dried under vacuum to provide about 200 mg of N-{3-[4-amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c]quinolin-l-yl]propyl}-4-methylbenzenesulfonamide as a white solid, m.p.231-232 C. Anal. calcd for C22H25N$O3S=0.20 CH4O: %C, 59.79; %H, 5.85;
%N, 15.70. Found: %C, 59.44; %H, 5.89; %N, 15.52.

Example 2 N-{3 -[4-Amino-2-(2-hydroxyethyl)-1 H-imidazo [4,5-c]quinolin-1-yl]propyl }
isoquinoline-3-carboxamide NHZ
NOH
N I ~
N

-~H
N N~
O -~
~ /

Boron tribromide (5.50 mL of 1 M in dichloromethane) was added dropwise to a chilled (0 C) suspension of1V-{3-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-l-yl]propyl}isoquinoline-3-carboxamide (1.0 g, 2.2 mmol; U.S.
Patent No.
6,756,382, Example 192) in dichloromethane (20 mL). The reaction mixture was stirred at 0 C for 45 minutes and then allowed to warm to ambient temperature. After 5 hours the reaction mixture was concentrated under reduced pressure and the residue was allowed to stand over the weekend. The residue was diluted with methanol (20 mL) and then heated to 50 C. Hydrochloric acid (about 10 mL of 6 N) was added and the mixture was stirred for about 2.5 hours. The mixture was made basic with aqueous sodium hydroxide and then extracted with ethyl acetate (x2). The combined extracts were dried over magnesium sulfate, filtered, and then concentrated under reduced pressure to provide a yellow solid.
This material was purified by prep HPLC (COMBIFLASH system eluting first with a gradient of 0 to 5% methanol in dichloromethane containing 1% ammonium hydroxide and then with a gradient of 5 to 10% methanol in dichloromethane containing 1%
ammonium hydroxide) to provide a white solid. This material was suspended in hot acetonitrile, allowed to cool, and then the solvent was decanted. The resulting material was dried under vacuum to provide about 400 mg of N-{3-[4-amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c]quinolin-l-yl]propyl}isoquinoline-3-carboxamide as a white solid, mp 245-246 C. Anal calcd for C25H24N602: %C, 67.73; %H, 5.59; %N, 18.80; Found:
%C, 67.38; %H, 5.54; %N, 18.84.

Example 3 N- {4-[4-Amino-2-(2-hydroxyethyl)-1 H-imidazo[4,5-c]quinolin-l-yl] butyl } methanesulfonamide N NOH
N

\-~ O
H ;Sw O
Part A
3-Methoxypropionyl chloride (15.4 g, 126 mmol) was added dropwise over a period of 20 minutes to a chilled (ice bath) solution of tert-butyl IV-{4-[(3-aminoquinolin-4-yl)amino]butyl}carbamate (38 g, 115 mmol, U.S. Patent No, 6,541,485, Example 2, Part B) in pyridine. The reaction mixture was stirred for 4 hours and then allowed to stand at ambient temperature over the weekend. Pyridine hydrochloride (3.9 g, 34 mmol) was added and the reaction mixture was heated at reflux overnight. The reaction mixture was concentrated under reduced pressure and the residue was diluted with dichloromethane (250 mL) and aqueous sodium bicarbonate (250 mL). The layers were separated.
The separatory funnel was rinsed with a small amount of methanol to remove a residue coating the walls. The combined organics were concentrated under reduced pressure. The residue was purified by prep HPLC (COMBIFLASH system eluting first with a gradient of 0 to 5% methanol in dichloromethane containing 1% ammonium hydroxide and then with a gradient of 5 to 10% methanol in dichloromethane containing 1% ammonium hydroxide) to provide 18 g of tert-butyl N-{4-[2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-l-yl]butyl } carbamate.
Part B
3-Chloroperoxybenzoic acid (20 g of 77%) was added in a single portion to a solution of the material from Part A (18 g, 45.2 mmol) in dichloroethane (170 mL). After 2 hours concentrated ammonium hydroxide (150 mL) was added and the reaction mixture was stirred until the phases were mixed well. Para-Toluenesulfonyl chloride (10.6 g, 54 mmol) was added in a single portion along with a sniall amount of dichloroethane. The reaction mixture was stirred overnight at ambient temperature and then diluted with water and dichloromethane. The layers were separated and the aqueous layer was extracted with dichloromethane (x2). The combined organics were dried over magnesium sulfate, filtered, and then concentrated under reduced pressure to provide 23 g of crude tert-butyl N-{4-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-l-yl]butyl}carbamate as a red tar.
Part C
The material from Part B was combined with a solution of hydrochloric acid in dioxane (325 mL of 4 M) and stirred at ambient tenlperature for 3 hours. The reaction mixture was concentrated under reduced pressure. The residue was dissolved in methanol (30 mL) and 6 M sodium hydroxide was added with stirring to about pH 9.
Attempts to extract with dichloromethane and ethyl acetate were not successful. The organic and aqueous layers were concentrated under reduced pressure and combined to provide a dark orange solid. This material was purified by prep HPLC (COMBIFLASH system eluting first with a gradient of 0 to 8% methanol in dichloromethane containing 1%
ammonium hydroxide and then with a gradient of 9 to 35% methanol in dichloromethane containing 1% ammonium hydroxide) to provide 10.65 g of 1-(4-aminobutyl)-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-4-amine as an orange solid.
Part D
Triethylamine (10.5 mL, 75.0 mmol) was added to a mixture of a portion (4.7 g, mmol) of the material from Part C in pyridine (50 mL). The reaction mixture was stirred for several minutes and then methanesulfonyl chloride (1.27 mL, 16.5 mmol) was added dropwise. The reaction mixture was stirred at ambient temperature for 2 hours and then at 50 C for 2 hours. More methanesulfonyl chloride (0.5 eq) was added and the reaction mixture was stirred at 50 C for 2 hours. Another portion of methanesulfonyl chloride (0.25 eq) was added and the reaction mixture was stirred at ambient temperature overnight. The reaction mixture was diluted with dichloromethane and water.
The layers were separated and the aqueous layer was extracted with dichloromethane (x3).
The combined organics were dried over magnesium sulfate, filtered, and then concentrated under reduced pressure to provide 5 g of crude N-{4-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]butyl}methanesulfonamide as a red oil.

Part E
Boron tribromide (22.4 mL of I M in dichloromethane) was added slowly to a chilled (ice bath) mixture of a portion of the material from Part D (3.5 g, about 8.9 mmol) and dichloromethane (50 mL). After the addition was complete the ice bath was removed and the reaction mixture was allowed to stir at ambient temperature for 3 hours. The reaction mixture was concentrated under reduced pressure. The residue was dissolved in methanol and then combined with hydrochloric acid (50 mL of 6 M). The mixture was stirred at 50 C for 2 hours and then concentrated under reduced pressure. The residue was combined with ammonia in methanol (about 50 mL of 7 M) to neutralize the acid and then concentrated. This procedure was repeated 3 times. The crude product was purified by prep HPLC (COMBIFLASH system eluting with a gradient of 0 to 10% methanol in dichloromethane containing 1% ammonium hydroxide). The product was stirred with hot acetonitrile, allowed to stand overnight, and then isolated by filtration, washed with acetonitrile, and dried in a vacuum oven to provide 1.1 g of N-{4-[4-amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]butyl}methanesulfonamide, mp 206-C. Anal caled for C17H23N503S: %C, 54.09; %H, 6.14; %N, 18.55. Found: %C, 53.83;
%H, 6.29; %N, 18.29.

Example 4 1-(2-Amino-2-methylpropyl)-2-hydroxymethyl-1 H-imidazo [4,5-c]quinolin-4-amine N N OH

-~NH2 Part A
Under a nitrogen atmosphere, triethylamine (6.6 mL, 47 mmol) was added slowly to a solution of 2,4-dichloro-3-nitroquinoline (10.0 g, 41.1 mmol) in anhydrous 1-methyl-2-pyrrolidinone (40 mL). The reaction mixture was cooled to 0 C with an ice bath. A
solution of 1,2-diamino-2-methylpropane (4.1 g, 47.3 mmol) in anhydrous 1-methyl-2-pyrrolidinone (5 mL) was added dropwise over a period of 15 minutes while maintaining the temperature of the reaction mixture below 4 C. After the addition was completed the ice bath was removed and the reaction mixture was allowed to stir at ambient temperature for 4 hours. The reaction mixture was slowly poured into vigorously stirred warm water (300 mL). The resulting suspension was stirred for 1 hour and then cooled to 13 C by adding ice. The solid was isolated by filtration and then washed with cold water until the filtrate was clear to provide 12.1 g of NI-(2-chloro-3-nitroquinolin-4-yl)-2-methylpropane-1,2-diamine as a damp yellow solid.
Part B
A solution of sodium hydroxide (1.8 g of solid sodium hydroxide dissolved in mL of water) was added slowly to a solution of the material from Part A(41.1 mmol) in tetrahydrofuran (96 mL). A solution of di-tert-butyl dicarbonate (10.8 g, 49.4 mmol) in tetrahydrofuran (30 mL) was added dropwise over a period of 15 minutes. The reaction solution was stirred at ambient temperature. After 6 hours 10% sodium hydroxide (2 mL) and additional di-tert-butyl dicarbonate (1.5 g) were added and the reaction solution was stirred at ambient temperature overnight. The layers were separated and the tetrahydrofuran was removed under reduced pressure to provide a mixture. The mixture was diluted with water (200 mL) and then extracted with dichloromethane (2 x 100 mL).
The organics were combined, washed sequentially with aqueous sodium carbonate (2 x 150 mL) and brine (100 mL), dried over sodiuni sulfate and magnesium sulfate, filtered, and then concentrated under reduced pressure. The residue was triturated with heptane (75 mL) for 15 minutes at 65 C and then filtered while hot. The isolated solids were washed with heptane (20 mL) to provide 13.2 g of tert-butyl N-{2-[(2-chloro-3-nitroquinolin-4-yl)amino]-1,1-dimethylethyl}carbamate as a yellow powdery solid.
Part C
A Parr vessel was charged with 5% Pt/C (0.5 g) and acetonitrile (10 mL). A
solution of the material from Part B in acetonitrile (450 mL) was added. The vessel was placed on a Parr shaker under hydrogen pressure (40 psi, 2.8 x 10$ Pa) for 5 hours. The reaction mixture was filtered through a layer of CELITE filter aid to remove the catalyst.
The filtrate was carried on to the next step.
Part D
The solution of tert-butyl N-{2-[(3-amino-2-chloroquinolin-4-yl)amino]-1,1-dimethylethyl}carbamate in acetonitrile from Part C was cooled to 5 C using an ice bath.
A solution of acetoxyacetyl chloride (4.8 g, 35.1 mmol) in acetonitrile (20 mL) was added dropwise at a rate sucli that the temperature of the reaction mixture was maintained at 5 C. After the addition was complete the ice bath was removed and the reaction mixture was allowed to stir at ambient temperature for 5 hours. The reaction mixture was concentrated under reduced pressure to provide 16.7 g of N-{2-[(3-acetoxyacetylamino-2-chloroquinolin-4-yl)amino]-1,1-dimethylethyl}carbarnate hydrochloride as a yellow powder.
Part E
A mixture of the material from Part D (15.7 g) and ammonia in methanol (235 mL
of 7 N) was divided into equal portions and placed in pressure vessels. The vessels were sealed, heated at 160 C for 20 hrs, and then allowed to cool to ambient temperature overnight. The reaction mixtures were filtered. The isolated solids were washed with water and dried in a vacuum oven at 60 C overnight to provide 6.0 g of a tan powder. A
portion (1 g) was treated with activated charcoal and recrystallized from ethanol (75 mL) to provide 0.5 g of 1-(2-amino-2-methylpropyl)-2-hydroxymethyl-lH-imidazo[4,5-c]quinolin-4-amine as a white granular solid, mp 248-250 C. Anal calcd for C15H19N50:
%C, 63.14; %H, 6.71; %N, 24.54. Found: %C, 63.13; %H, 6.81; %N, 24.64.

Example 5 N-[2-(4-Amino-2-hydroxymethyl-1 H-imidazo[4,5-c]quinolin-l-yl)-1,1-dimethylethyl]cyclohexanecarboxamide N

ll N P
H
O
A solution of 1-(2-amino-2-methylpropyl)-2-hydroxymethyl-lH-imidazo[4,5-c]quinolin-4-amine (2.0 g, 7.0 mmol) in 1-methyl-2-pyrrolidinone (30 mL) was cooled to -20 C. Triethylamine (1.1 mL, 7.7 mmol) was added in a single portion. A
chilled (-5 C) solution of cyclohexanecarbonyl chloride (1.03 g, 7.0 mmol) in 1-methyl-2-pyrrolidinone (2 mL) was added dropwise over a period of 20 minutes while maintaining the reaction mixture at -20 C. The reaction mixture was stirred at ambient temperature overnight. Additional cyclohexanecarbonyl chloride (0.1 g) was added and the reaction mixture stirred for 2 hours. The reaction mixture was poured into water with vigorous stirring. The resulting precipitate was isolated by filtration to provide 1.7 g of an ivory powder. Analysis by high performance liquid chromatography and NMR indicated that the powder was a mixture of the desired product and an ester formed from the reaction of the hydroxy group of the desired product with cyclohexanecarbonyl chloride.
The powder was dissolved in ethanol (25 mL), combined with a solution of sodium hydroxide (0.21 g) in water (25 mL), and then heated at 50 C for 3 hours. The ethanol was removed under reduced pressure and the solids were isolated by filtration to provide 1.2 g of a light tan powder. The powder was dissolved in a mixture of acetonitrile (100 mL), water (2 mL) and ethanol (25 mL). The solution was allowed to stand overnight and was then concentrated to a volume of 5 mL to provide a white paste. The paste was triturated with warm (70 C) acetonitrile (50 mL) for 30 minutes, heated to reflux, and then allowed to cool to ambient temperature. The resulting solid was isolated by filtration to provide 1.05 g ofN-[2-(4-amino-2-hydroxymethyl-lH-imidazo[4,5-c]quinolin-l-yl)-1,1-dimethylethyl]cyclohexanecarboxamide as a light yellow powder, mp 248-250 C.
Anal calcd for C22H29N502: %C, 66.81; %H, 7.39; %N, 17.71; Found: %C, 66.56;
%H, 7.60; %N, 17.82.

Example 6 N-{2-[4-Amino-2-(2-hydroxyethyl)-1 H-imidazo[4,5-c]quinolin-l-yl]-l, l -dimethylethyl } methanesulfonamide NHZ
N N' ~-OH
~}-./
N

~
NH O
S' O~' \
Part A

Triethylamine (39.3 mL, 0.282 mol) was added to a chilled (ice bath) solution of Nl-(2-chloro-3-nitroquinolin-4-yl)-2-methylpropane-1,2-diamine (41.42 g, 0.141 mol) in dichloromethane (about 500 mL). Under a nitrogen atmosphere a solution of methanesulfonic anhydride in (29.47 g, 0.169 mol) in dichloromethane (100 mL) was added via a cannula to the reaction mixture over a period of 45 minutes. After the addition was complete the ice bath was removed and the reaction mixture was allowed to stir at ambient temperature overnight. The reaction mixture was washed sequentially with saturated aqueous sodium bicarbonate (x2) and brine, dried over a mixture of sodium sulfate and magnesium sulfate, filtered, and then concentrated under reduced pressure to provide 46.22 g of an orange solid. This material was recrystallized from toluene (about 1 L), isolated by filtration, rinsed with cold toluene, and dried under high vacuum at 60 C
to provide 33.09 g of N-{2-[(2-chloro-3-nitroquinolin-4-yl)amino]-1,1-dimethylethyl } methanesulfonamide.
Part B
A hydrogenation vessel was charged with 5% Pt/C (4.14 g) and a solution of 1V-{2-[(2-chloro-3-nitroquinolin-4-yl)amino]-1,1-dimethylethyl}methanesulfonamide (54.59 g, 0.147 mol) in acetonitrile (1800 mL). The vessel was placed under hydrogen pressure (48 psi, 3.3 x 105 Pa) overnight. An additional portion (4.25 g) of catalyst was added and the vessel was placed under hydrogen pressure (48 psi, 3.3 x 105 Pa) for 4 hours.
The reaction mixture was filtered through a layer of CELITE filter aid and the filter cake was rinsed with fresh acetonitrile until the washes were clear.
Part C

Under a nitrogen atmosphere, 3-methoxypropionyl chloride (17.6 mL, 0.162 mol) was added dropwise to the solution ofN-{2-[(3-amino-2-chloroquinolin-4-yl)amino]-1,1-dimethylethyl}methanesulfonamide (0.147 mol) in acetonitrile (2.2 L) from Part B. The reaction mixture was allowed to stir at ambient temperature over the weekend.
The resulting precipitate was isolated by filtration, rinsed with a small amount of acetonitrile, and then dried under high vacuum at 60 C to provide 55.84 g of N-{2-chloro-4-[2-(methanesulfonylamino)-2-methylpropyl] quinolin-3 -yl } -3 -methoxypropionamide.
Part D
A Parr bomb was charged with 25.0 g of 1V-{2-chloro-4-[2-(methanesulfonylamino)-2-methylpropyl] aminoquinolin-3 -yl } -3 -methoxypropionamide and ammonia in methanol (300 mL of 7 N). A second vessel was charged with 30.21 g of N- { 2-chloro-4- [2-(methanesulfonylamino)-2-methylpropyl] quinolin-3 -yl } -3 -methoxypropionamide and ammonia in methanol (400 mL of 7 N). Both vessels were sealed and then heated at 170 C for 14 hours. The reaction mixtures were combined and the solvent was removed under reduced pressure. The residue was partitioned between dichloromethane and saturated aqueous sodium bicarbonate. The organic layer was washed sequentially with saturated aqueous sodium bicarbonate and brine, dried over sodium sulfate, filtered, and then concentrated under reduced pressure to provide 38.16 g of N- {2-[4-amino-2-(2-methoxyethyl)-1 H-imidazo[4,5-c]quinolin-l-yl]-1,1-dimethylethyl}methanesulfonamide as an off white foam.
Part E
Under a nitrogen atmosphere, boron tribromide (3.5 mL of 1 M in dichloromethane) was added dropwise to a chilled (0 C) solution of N-{2-[4-amino-2-(2-methoxyethyl)-1 H-imidazo [4,5-c]quinolin-l-yl]-1,1-dimethylethyl }
methanesulfonamide (0.55 g, 1.40 mmol) in dichloromethane (20 mL). The reaction was allowed to warm to ambient temperature overnight. The reaction was quenched with methanol (10 mL) and the solvent was removed under reduced pressure. The residue was dissolved in hydrochloric acid (6 N), stirred at 50 C for about 2.5 hours, and then allowed to cool to ambient temperature. The reaction mixture was adjusted to pH 11 with ammonium hydroxide and then extracted with dichloromethane (x 10). The combined organics were washed with brine, dried over sodium sulfate, filtered, and then concentrated under reduced pressure to provide 0.47 g of a white solid. This material was purified by prep HPLC (HORIZON HPFC system, eluting with a gradient of 30-50% CMA in chloroform for 15 column volumes followed by 50% CMA in chloroform for 5 colunm volumes) and then dried under high vacuum to provide 250 mg of N-{2-[4-amino-2-(2-hydroxyethyl)-1H imidazo[4,5-c]quinolin-l-yl]-1,1-dimethylethyl}methanesulfonamide as white solid, m.p. 209 - 212 C. 'H NMR (500 MHz, DMSO-d6) 6 8.30 (d, J= 8.2 Hz, 1H), 7.60 (d, J=
8.2 Hz, 1H), 7.39 (m, 1H), 7.27 (s, 1H), 7.21 (m, 1H), 6.49 (s, 2H), 4.84 (t, J= 5.4 Hz, 2H), 4.82 (br s, 1H), 3.88 (m, 2H), 3.18 (br s, 2H), 3.00 (s, 3H), 1.27 (br s, 6H); 13C NMR

(125 MHz, DMSO-d6) 8 153.6, 152.0, 145.4, 133.5, 126.9, 126.8, 126.5, 121.3, 120.8, 115.6, 60.5, 57.9, 54.1, 44.8, 31.4, 25.8; MS (ESI) m/z 378 (M + H)+; Anal, calcd for C17H23N5O3S: %C, 54.09; %H, 6.14; %N, 18.55. Found: %C, 53.76; %H, 6.02; %N, 18.32.

Example 7 N-[2-(4-Amino-2-hydroxymethyl-lH-imidazo[4,5-c]quinolin-l-yl)-1,1-dimethylethyl]methanesulfonamide N OH
N N

NH~,O
O11'S\
Part A
A pressure vessel was charged with a solution of of.N-{2-[(2-chloro-3-nitroquinolin-4-yl)amino]-1,1-dimethylethyl}methanesulfonamide (5 g, 13 mmol) in acetonitrile (150 mL). Catalyst was added (0.5 g of 5% Pt/C) and the vessel was placed under hydrogen pressure (50 psi, 3.4 X 105 Pa) for 2 hours. The reaction mixture was filtered through a layer of CELITE filter aid.
Part B
The solution of N-{2-[(3-amino-2-chloroquinolin-4-yl)amino]-1,1-dimethylethyl}methanesulfonamide in acetonitrile from Part A was chilled in an ice bath.
Acetoxyacetyl chloride (1.5 mL, 14 mmol) was added over a period of 5 minutes.
The reaction mixture was allowed to stir for 3 hours. A precipitate was isolated by filtration and rinsed with acetonitrile to provide crude N-{2-chloro-4-[2-(methanesulfonylamino)-2-methylpropyl]quinolin-3-yl} acetoxyacetamide hydrochloride.
Part C
A solution of sodium hydroxide (0.8 g) in water (15 mL) was added to a suspension of the material from Part B in ethanol (60 mL) until all of the solid dissolved.
The reaction mixture was heated at 60 C overnight and then concentrated under reduced pressure. The residue was dissolved in water (50 mL), sodium chloride (10 g) was added, and the mixture was extracted with chloroform (3 x 300 mL). The extracts were concentrated under reduced pressure to provide about 4 g of crude N-[2-(4-chloro-2-hydroxymethyl-lH-imidazo[4,5-c]quinolin-1-yl)-1,1-dimethylethyl]methanesulfonamide.
Part D
The material from Part C was combined with a solution of ainmonia in methanol (50 mL of 7 N) and heated at 150 C for 10 hours. The reaction mixture was allowed to cool to ambient temperature. A precipitate was isolated by filtration, rinsed with methanol (20 mL), slurried with water (50 mL), isolated by filtration, washed with water (20 mL), and dried to provide 2.7 g of a brown crystalline solid. This material was combined with methanol (50 mL), heated at 50 C overnight, and then isolated by filtration to provide 2.3 g ofN-[2-(4-amino-2-hydroxymethyl-lH-imidazo[4,5-c]quinolin-1-yl)-1,1-dimethylethyl]methanesulfonamide, mp 262-265 C. Anal, calcd for C16H21N503S:
%C, '52.88; %H, 5.82; %N, 19.27. Found: %C, 52.64; %H, 5.95; %N, 19.50.

Examples 8 - 72 Part A
A reagent (1.1 eq) from Table 1 below was added to a test tube containing a solution of 1-(4-aminobutyl)-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-4-amine (73 mg) in 1V,N-dimethylacetamide (1 mL) containing N,N-diisopropylethylamine (2 eq). The test tube was placed on a shaker overnight. The solvent was removed by vacuum centrifugation. The reaction mixtures were separated by solid-supported liquid-liquid extraction according to the following procedure. Each sample was dissolved in chloroform (1 mL) then loaded onto diatomaceous earth that had been equilibrated with de-ionized water (600 L) for about 20 minutes. After 10 minutes chloroform (500 L) was added to elute the product from the diatomaceous earth into a well of a collection plate. After an additional 10 minutes the process was repeated with additional chloroform (500 L). The solvent was then removed by vacuum centrifugation.
Part B
The residue (in a test tube) was combined with dichloromethane (1 mL) and the mixture was sonicated to dissolve the solids. The solution was cooled (0 C) and then combined with boron tribromide (400 L of 1 M in heptane). The mixture was shaken for 5 minutes, placed in an ice bath for 30 minutes, and then shaken overnight.
The solvents were removed by vacuum centrifugation. The residue was diluted with methanol (1 mL) and hydrochloric acid (500 L of 6 N). The mixture was shaken for 30 minutes and then the solvents were removed by vacuum centrifugation. The compounds were purified by preparative high performance liquid chromatography (prep HPLC) using a Waters FractionLynx automated purification system. The prep HPLC fractions were analyzed using a Waters LC/TOF-MS, and the appropriate fractions were centrifuge evaporated to provide the trifluoroacetate salt of the desired compound. Reversed phase preparative liquid chromatography was performed with non-linear gradient elution from 5-95% B
where A is 0.05% trifluoroacetic acid/water and B is 0.05% trifluoroacetic acid/acetonitrile. Fractions were collected by mass-selective triggering.
Table 1 below shows the reagent used for each example, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

Table 1 NHz N ~ C NOH
~
~ N
~ /
/
H'R
Measured Example Reagent R Mass (M+H) 8 None ~ H 300.1840 9 Cyclopropanecarbonyl chloride 368.2063 O
Isobutyryl chloride ~CH3 370.2224 11 Pi valoyl chloride ~,CH3 384.2390 12 Benzoyl chloride -- 404.2103 O

13 Phenyl chloroformate p ~ 0 ~ 420.2056 O

14 3-Cyanobenzoyl chloride 429.2031 N
O

15 Hydrocinnamoyl chloride 432.2377 O
Isonicotinoyl chloride 16 hydrochloride 405.2071 N
O
Nicotinoyl chloride 17 hydrochloride N 405.2058 -- O

18 Methanesulfonyl chloride S,CH3 378.1592 O

19 Ethanesulfonyl chloride ~ 392.1729 Q,O
S

20 1 -Propanesulfonyl chloride 406.1899 C; Q

21 Isopropylsulfonyl chloride /-406.1888 22 Dimethylsulfamoyl chloride 407.1853 H3C,N-CH3 0,0 S<

23 1-Butanesulfonyl chloride \-~ 420.2050 OO

24 Benzenesulfonyl chloride 440.1741 25 1-Methylimidazole-4-sulfonyl 444.1806 chloride N
\~,- N.CH

Do -_ S

26 3-Methylbenzenesulfonyl 54.1895 chloride b O

27 alpha-Toluenesulfonyl chloride 454.1923 ,O

28 o-Toluenesulfonyl chloride H C /\ 454.1944 ~
0J,O

29 p-Toluenesulfonyl chloride / \ 454.1907 30 2-Fluorobenzenesulfonyl chloride F 458.1664 0,0 31 3-Fluorobenzenesulfonyl chloride b 458.1652 F
0,0 -~S

32 4-Fluorobenzenesulfonyl chloride 458.1639 F

Op 33 3-Cyanobenzenesulfonyl chloride 465.1678 N
O,p 34 4-Cyanobenzenesulfonyl chloride 465.1668 N

35 beta-Styrene sulfonyl cliloride 466.1895 p ~S"
2,5-Dimethylbenzenesulfonyl 36 chloride H3C ~ \ 468.2063 ~ CH3 Op ~S

37 3,5-Dimethylbenzenesulfonyl b~-CH3 468.2046 chloride H3C

38 2-Chlorobenzenesulfonyl 474.1351 chloride CI / \
-O ,p 39 3-Chlorobenzenesulfonyl / 474.1385 chloride CI
O p 40 4-Chlorobenzenesulfonyl S \ 474.1390 chloride CI

41 1-Naphthalenesulfonyl chloride Cb 490.1891 42 2-Naphthalenesulfonyl chloride 490.1885 OO
~S"

43 (Trifluoromethyl)benzenesulfonyl F F / \ 508.1592 chloride F

44 (Trifluoromethyl)benzenesulfonyl 508.1612 chloride F
F F

45 (Trifluoromethyl)benzenesulfonyl q-F 508.1640 chloride F

F

-, >

46 2,3-Dichlorobenzenesulfonyl 508.0967 chloride CI

CI
~ 0 0 47 2,4-Dichlorobenzenesulfonyl CI \ ~ 508.0979 chloride /
CI

48 2,5-Dichlorobenzenesulfonyl CI 508.0987 chloride CI

49 2,6-Dichlorobenzenesulfonyl CI 508.0968 chloride ci t OO

50 3,4-Dichlorobenzenesulfonyl 508.0961 chloride _ CI
CI

51 3,5-Dichlorobenzenesulfonyl 508.0985 chloride CI
CI

52 Methyl isocyanate \ 357.2073 O

53 Ethyl isocyanate N 371.2203 O

54 Isopropyl isocyanate N CH3 385.2347 C

55 n-Propyl isocyanate H~ 385.2349 C

56 n-Butyl isocyanate H 399.2494 57 sec-Butyl isocyanate H-~CH3 399.2517 _ CH3 O

58 Cyclopentyl isocyanate N--O 411.2516 H

~S

59 Cyclopropylmethyl N 413.2133 isothiocyanate H

60 Phenyl isocyanate N ~ 419.2226 H \ ~

61 Cyclohexyl isocyanate N 425.2701 H~

N
N

62 Benzyl isocyanate H 433.2374 ~
\ /

63 m-Tolyl isocyanate H 433.2344 64 Benzoyl isocyanate H 447.2126 ~
\ /

65 2-Phenyl ethylisocyanate N 447.2512 66 4-Chlorophenyl isocyanate N ' 453.1797 H \ / ci 67 trans-2-Phenylcyclopropyl 0 459.2518 isocyanate N
H

68 N,N-Dimethylcarbamoyl chloride N_CH 371.2185 ~

69 1-Pyrrolidinecarbonyl chloride N0 397.2382 70 1-Piperidinecarbonyl chloride N 411.2526 ~Th) 71 4-Morpholinylcarbonyl chloride 413.2330 72 1V-Methyl-IV-phenylcarbamoyl N ~ 433.2364 chloride H3~ ~ /

Examples 73 - 110 Part A
Tert-Butyl 3-[4-amino-2-(2-methoxyethyl)-1 H-imidazo [4, 5-c] quinolin-l-yl]propylcarbamate ( 5 g, U.S. Patent No. 6,573,273, example 148) and hydrochloric acid in dioxane (100 mL of 4 M) were combined and stirred for 4 hours at ambient temperature. The reaction mixture was concentrated under reduced pressure. The residue was dissolved in methanol (30 mL). The pH was adjusted to pH 8 with 6 M sodium hydroxide. The solution was diluted with dichloromethane, ethyl acetate, triethylamine, and brine. The organic layer was concentrated under reduced pressure to provide an orange solid. This material was purified by prep HPLC (COMBIFLASH system eluting first with a gradient of 0 to 10% methanol in dichloromethane containing 1%
ammonium hydroxide and then with a gradient of 9 to 30% methanol in dichloromethane containing 1% ammonium hydroxide) to provide 1.58 g of 1-(3-aminopropyl)-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-4-amine as a yellow solid.
Part B
A reagent (1.1 eq) from Table 2 below was added to a test tube containing a solution of 1-(3-aminopropyl)-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-4-amine (30 mg) in chloroform (1 mL) containing N,N-diisopropylethylamine (1.5 eq).
The test tube was placed on a shaker overnight. The reaction mixtures were separated by solid-supported liquid-liquid extraction according to the following procedure. Each reaction mixture was loaded onto diatomaceous earth that had been equilibrated with de-ionized water (600 L) for about 20 minutes. After 10 minutes chloroform (500 L) was added to elute the product from the diatomaceous earth into a well of a collection plate. After an additional 10 minutes the process was repeated with additional chloroform (500 L). The solvent was then removed by vacuum centrifugation.

Part C
The ether was cleaved and the resulting product was purified using the method of Part B in Examples 8 - 72. Table 2 below shows the reagent used for each example, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

Table 2 NOH
N I ~
N

N
R
Example Reagent R Measured Mass (M+H) 73 None H 286.1689 74 Propionyl chloride 01- 342.1956 75 Cyclopropanecarbonyl 354.1946 chloride 0 76 Butyryl chloride p 356.2122 77 Isobutyryl chloride 0~-(CH3 356.2119 78 Cyclobutanecarbonyl 368.2120 chloride O

79 3-Chlorobenzoyl O
chloride Q 424.1570 CI

80 4-Chlorobenzoyl ~' 424.1583 chloride O / CI

81 Nicotinoyl chloride O O-N\
391.1913 hydrochloride trans-2-Phenyl-l-82 cyclopropanecarbonyl 430.2257 chloride Methanesulfonyl 0 83 chloride p" CH 364.1479 84 Ethanesulfonyl g'P
378.1639 chloride ~

1-Propanesulfonyl O S
chloride O" --_cH3 392.1783 86 Isopropylsulfonyl O g CH3 392.1788 chloride (J' ~H3 87 Dimethylsulfamoyl O S'N CH3 393.1715 chloride O' CH3 1-Butanesulfonyl O S
88 chloride O 406.1946 89 Benzenesulfonyl O g /~ 426.1633 chloride 0 2,2,2- O S
Trifluoroethanesulfonyl O' 432.1355 chloride F F F

91 Methylbenzenesulfonyl o S /\ 440.1774 chloride Og 92 alpha-Toluenesulfonyl 440.1762 chloride 93 p- Toluenesulfonyl O; /\ 440.1790 chloride 0 94 Fluorobenzenesulfonyl ~ S 444.1523 chloride Fluorobenzenesulfonyl ~ S F 444.1545 chloride ---3- , N
96 Cyanobenzenesulfonyl O g /\ 451.1554 chloride O
-97 Cyanobenzenesulfonyl ~S \ 451.1582 chloride / =N

H
98 Ethyl isocyanate 0 N 357.2050 \-CH3 ~_H
99 Isopropyl isocyanate 0 N 371.2234 H3C/\-CH3 ~_H
N
100 n-Butyl isocyanate O 385.2364 \-~CH3 H
101 Cyclopentyl isocyanate 0 397.2359 Cyclopropylmethyl N
102 isothiocyanate s 399.1979 H
N
103 Phenyl isocyanate O ~ 405.2040 ~H
N
104 Cyclohexyl isocyanate O 411.2526 b~H

105 Benzyl isocyanate O N ~ 419.2239 ~ /

~H
O
trans-2-106 Phenylcyclopropyl 445.2388 isocyanate 107 1-Piperidinecarbonyl N 397.2384 chloride 0 ~
108 4-Morpholinylcarbonyl 399.2173 ~O
chloride 0 4-Methyl-l-109 piperazinecarbonyl ON 412.2485 chloride ~~-CH3 N-Methyl-N- o~-N(C CH3 110 phenylcarbamoyl 419.2229 chloride Examples 111 -140 Boron tribromide (400 L of 1 M in heptane) was added to a tube containing a chilled (0 C) solution of a compound of Formula Xa (about 25 mg) in dichloromethane (1 mL). The tube was vortexed, maintained at 0 C for 0.5 hour, and then shaken overnight at ambient temperature. The reaction mixture was diluted with methanol (1 mL) and hydrochloric acid (250 L of 6 N), vortexed, and then the solvents were removed by vacuum centrifugation. The compounds were purified by prep HPLC as described in Examples 8 - 72. Table 3 shows the structure of the starting material, a reference for the starting material, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

+ N ~
G N "0 I

Z-Z \ _ LL
O \ I p:cn \
z z- z Z=
zz ~

F-+

O

Z-Z cu X
Z
Z- \

~
a) ZZrnN
m N ~

a~ aM
W v; k ~
~ '. N

rn ~n o rn ~n o0 M ~
N cV

tl- o tn cm M d d M I \
r'OO = O lO
~ z rz11-1 0 Z= 2 ru o0 Z~ -M Z~N ~NN
OC " +- 00 ~ 'n 'n Q) v;
v~~ ~c W r~~ k v~~ x M d t/l ~O
,--- - .--.--r .-~ '--r r--a 00 N ~O
N N N --~
kr) ~ ~1m 00 _ ~ U~O
Z ~ ~ \ 0 z= /ZI
~ ~
z N
z~ z ~
M

aLr, akn akD
vs X

O~ N
l~ 00 r-. r--r-, 60 M ~f O l~
~
N N N
d d -+ 00 o l~ o Z2 ,p zz~
2z U
= jJj0 = sz U
U

ZNO~0 7~,~-~+M~ ~NO, ++ oo ''" +~-- N +t- ~ +~ 00 M Q) kn~ C,3 v; ~ ~C v~ ?C vi ~ >C v~ "o X
~D

N M d N N N N

~ 00 N d ~ 0~0 M t~ QN
trl O O~ d l~ 00 Ni' 01 ~
M M d M M
cl) 2 =
= U V O
/1 c 1 N
o / z cn I J = Z

O
O zZO o =

Z~'~ zM~ 7o~N Zcry~ Z ~ d Q" ~ c*1 Q ~ l~ Q" ~ M Q t4 N

N n N ' M M d \ ~ _ O =
I n z 2Z i = ZZ~~U U
M J
2 =z~0 U = MZ p Ir U U p _M
z z M~ ~ p, Z N z oo cn +~ - +rd N oo~ -~oo Cd vs~ v~~ v~ ~
sc cri~
O - N M
M ('rl M M
'--i --~ .--.

rn ~ ~ ~ N
'- lp M 00 0 N N
d O O tY i ~O
d ~t M M M
M z = "
z M ~ ~ =p ~U U U
U U Uc~=O p Z= p ~ o p zz r ~ O jZI
-'r 6 o ci 6 6 z',00 z~ ~ z~, zo N N C,q la, aa ~ cj ~ ~ a~ w M a O~O
x Ln C/) v~ v3 cn I'D v) Ln 00 M M M M
,--i .--i '--i '--r+

rn ~

V'1 0 U
..r i--N
rn N
O
.--U

V
N
b~A
.~
~
"O
~
2 ~
~
V "O O
z COS ~
z= ~
~
~
~
=z o ~
r-j ~O O

U
Q) 'C3 N
.--~
U
O - N
Z ~
~ M cd ~ a~ v +c~ P"' vi p~ G~
C/j -o P-+ p ~D N FO
~'bA

,--,--+'~'-.--~

Example 141 N- { 3 - [4-Amino-2-(2-hydroxyethyl)-1 H-imidazo [4, 5 -c] quinolin-1-yl]propyl } -2-methylpropionamide NHa N N, OH
N

-~ N
O1-~
Part A

1-(3-Aminopropyl)-2-(2-methoxyethyl)-1 H-imidazo [4,5-c]quinolin-4-amine dihydrochloride (6 g, 16 mmol) was combined with triethylamine (11.2 mL, 80 mmol) and pyridine (100 mL). Isobutyryl chloride (1.9 g, 18 mmol) was added dropwise and the reaction mixture was stirred at ambient temperature for 1 hour. The reaction mixture was combined with saturated aqueous sodium bicarbonate and extracted with dichloromethane (3 x 200 mL). The combined organics were dried over magnesium sulfate, filtered through a layer of CELITE filter aid, and then concentrated under reduced pressure to provide 6.2 g of crude N-{3-[4-amino-2-(2-methoxyethyl)-1H-imidazo[4,5-c]quinolin-l-yl]propyl}-2-methylpropionamide as a brown solid.
Part B

The material from Part A was combined with dichloromethane (40 mL), stirred until homogeneous, and then chilled in an ice bath. Boron tribromide (40 mL of 1 M in dichloromethane) was slowly added. The ice bath was removed and the reaction mixture was stirred overnight at ambient temperature. The reaction mixture was concentrated under reduced pressure. The residue was combined with methanol (50 mL) and hydrochloric acid (50 mL of 6 N) and heated at 50 C for 2 hours. The solution was adjusted to pH 9 with sodium hydroxide (6 M) and then extracted first with ethyl acetate (3 x 100 mL) and then with dichloromethane. The organics were dried over magnesium sulfate, filtered through a layer of CELITE filter aid, and then concentrated under reduced pressure. The residue was purified by prep HPLC (HORIZON HPFC system, eluting with a gradient of 0-10% methanol in dichloromethane), recrystallized from acetonitrile, and then dried in a vacuum oven to provide 208 mg of N-{3-[4-amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c]quinolin-1-yl]propyl}-2-methylpropionamide as an off-white solid, mp 196-198 C. Anal. calcd for CjQH25N502: %C, 64.20; %H, 7.09; %N, 19.70; Found: %C, 63.99; %H, 7.28; %N, 19.63.

Example 142 1-[2-(4-Amino-2-hydroxymethyl-1 H-imidazo [4,5-c]quinolin-l-yl)- l,1-dimethylethyl]-3-(1-methylethyl)urea N~ ~ N OH
N
NN

O
Part A
Under a nitrogen atmosphere, a solution of 1,2-diamino-2-methylpropane (52.20 mL, 503.3 mmol), triethylamine (131.8 mL, 958.8 mmol), and dichloromethane (1.0 L) was chilled in an ice water bath. 4-Chloro-3-nitroquinoline (100.0 g, 479.4 mmol) was added in portions over a period of 5 minutes. The reaction mixture was stirred at 0 C for 2 hours and then allowed to slowly warm to ambient temperature. After 16 hours the reaction mixture was concentrated under reduced pressure. The residue was triturated with water (500 mL) for 1 hour. The resulting solid was isolated by filtration and dried overnight in a vacuum desiccator to provide 124.6 g ofNl-(3-nitroquinolin-1-yl)-2-methylpropane-1,2-dianline as a yellow crystalline solid.
Part B
Under a nitrogen atmosphere, a suspension ofNl-(3-nitroquinolin-l-yl)-2-methylpropane-1,2-diamine (60.0 g, 231 mmol) in dichloromethane (1.0 L) was chilled in an ice bath. Isopropyl isocyanate (23.8 mL, 242 mmol) was added dropwise over a period of 10 minutes. The reaction was allowed to slowly warm to room temperature.
After 17 hours additional isopropyl isocyanate (about 2 mL) was added. After an additional 3 hours more isopropyl isocyanate (1 mL) was added. After 2 more hours the reaction mixture was concentrated under reduced pressure to provide 79. 8 g of 1- { 1,1-dimethyl-2-[(3-nitroquinolin-l-yl)amino]ethyl}-3-(1-methylethyl)urea as a bright yellow solid.

Part C
A pressure vessel was charged with the material from Part B, 5% Pt/C (4.24 g), and acetonitrile (1.5 L). The mixture was placed under hydrogen pressure for 20 hours and then filtered through a layer of CELITE filter aid. The filter calce was rinsed with additional acetonitrile. The filtrate was concentrated under reduced pressure.
The residue was dissolved in toluene (750 mL) and then concentrated under reduced pressure to remove residual water. The toluene concentration was repeated. The residue was dissolved in dichloromethane (about 1 L), concentrated under reduced pressure, and then dried under high vacuum to provide 66.4 g of 1-{1,1-dimethyl-2-[(3-aminoquinolin-l-yl)amino]ethyl}-3-(1-methylethyl)urea as an orange foam.
Part D
Under a nitrogen atmosphere, a solution of 1-{1,1-dimethyl-2-[(3-aminoquinolin-1-yl)amino]ethyl}-3-(1-methylethyl)urea (66.0 g, 209 inmol) and triethylamine (32.1 mL, 230 mmol) in dichloromethane (1.0 L) was chilled in an ice bath. Ethoxyacetyl chloride (23.6 mL, 291 mmol) was added dropwise over a period of 10 minutes. The reaction mixture was allowed to slowly warm to ambient temperature overnight. The reaction mixture was concentrated under reduced pressure. The residue was combined with butanol (800 mL) and triethylamine (87 mL, 627 mmol) and heated at 140 C for 3 hours.
The reaction mixture was cooled to ambient temperature and then concentrated under reduced pressure to provide a light brown foam. This material was purified by column chromatography (silica gel, eluting with 98/2/0.5 chloroform/methanol/ammonium hydroxide) to provide 29.36 g of 1-[2-(2-ethoxymethyl-lH-imidazo[4,5-c]quinolin-l-yl)-1,1-dimethylethyl]-3-(1-methylethyl)urea as a light yellow foam.
Part E
3-Chloroperoxybenzoic acid (26.33 g of 60%, 91.56 mmol) was added in portions over a period of 5 minutes to a chilled solution of the material from Part D
in chloroform (350 mL). The reaction mixture was allowed to slowly warm to ambient temperature.
After 2 hours the reaction mixture was chilled in an ice bath and ammonium hydroxide (100 mL) was added with vigorous stirring to homogenize. Para-toluenesulfonyl chloride (15.27 g, 80.12 mmol) was added in portions over a period of 10 minutes. The ice bath was removed and the reaction mixture was stirred for 30 minutes. The reaction mixture was diluted with water (100 mL) and chloroform (250 mL). The layers were separated.

The organic layer was washed with 10% sodium carbonate (200 mL) and water (200 mL).
The combined aqueous was back extracted with chloroform (100 mL). The combined organics were washed with brine (200 mL), dried over magnesium sulfate, filtered, and then concentrated under reduced pressure to provide a light brown foam. The foam was purified by column chromatography (silica gel, eluting with 95/5 chloroform/methanol) and then recrystallized from acetonitrile to provide 3.75 g of 1-[2-(4-amino-2-ethoxymethyl-lH-imidazo[4,5-c]quinolin-1-yl)-1,1-dimethylethyl]-3-(1-methylethyl)urea as an off white solid.
Part F
Under a nitrogen atmosphere, a suspension of 1-[2-(4-amino-2-ethoxymethyl-lH-imidazo[4,5-c]quinolin-l-yl)-1,1-dimethylethyl]-3-(1-methylethyl)urea (1.19 g, 2.99 mmol) in dichloromethane (30 mL) was chilled in an ice bath. Boron tribromide (7.47 mL
of 1 M in dichloromethane) was added. The reaction mixture was allowed to warm slowly to ambient temperature and then stirred for 18 hours. Additional boron tribromide (2 eq) was added. After 2 hours the reaction mixture was diluted with acetonitrile (10 mL) and the reaction mixture was stirred overnight. The reaction mixture was diluted with dichloromethane (10 mL) and acetonitrile (10 mL), stirred for an additional 16 hours, quenched with methanol (25 mL), and then concentrated under reduced pressure to provide an orange foam. The foam was dissolved in hydrochloric acid (25 mL of 6 N) and heated at 50 C for 2 hours. The solution was neutralized with 50% sodium hydroxide.
The resulting gummy precipitate was extracted with chloroform (3 x 15 mL). The combined organics were washed with brine (15 mL), dried over magnesium sulfate, filtered, and then concentrated under reduced pressure to provide an off white solid. This material was purified by prep HPLC (HORIZON HPFC system, eluting with a gradient of 15-50% CMA in chloroform) and then recrystallized from acetonitrile to provide 335 g of 1-[2-(4-amino-2-hydroxymethyl-l H-imidazo [4,5-c]quinolin-1-yl)-1,1-dimethylethyl]-3-(1-methylethyl)urea as a white crystalline solid, mp 196-199 C; 'H NMR (300 MHz, DMSO-d6) S 8.38 (d, J= 8.0 Hz, 1 H), 7.59 (d, J= 7.5 Hz, 1 H), 7.43-7.38 (m, 1 H), 7.24-7.19 (m, 1 H), 6.54 (s, 2 H), 5.72 (s, 1 H), 5.63 (d, J= 7.6 Hz, 1 H), 5.46 (t, J= 5.7 Hz, 1 H), 5.01 (s, 2 H), 4.78 (s, 2 H), 3.78-3.67 (m, 1 H), 1.17 (bs, 6 H), 1.05 (d, J= 6.9 Hz, 6 H); 13C NMR (75 MHz, DMSO-d6) S 157.2, 154.2, 152.3, 145.6, 134.3, 126.8, 126.7, 121.5, 120.9, 115.8, 56.5, 54.2, 52.1, 26.4, 23.6; MS (APCI) m/z 371 (M + H)+;
Anal.

Calcd for C19H26N602-0.3Ha0: %C, 60.72; toH, 7.13; %N, 22.36; Found: %C, 60.44; %H, 7.42; %N, 22.52.

Example 143 {4-Amino-l-[2,2-dimethyl-3-(methylsulfonyl)propyl]-1H-imidazo[4,5-c]quinolin-2-yl}methanol NHZ
N OH
N~ O

O To a suspension of 1-[2,2-dimethyl-3-(methylsulfonyl)propyl]-2-(ethoxymethyl)-1H-imidazo[4,5-c]quinolin-4-amine (0.4 g, 1.02 mmol) in dichloromethane (5 mL) was added boron tribromide (5.1 mL, 1 M solution in dichloromethane). An exotherm was observed upon addition and the mixture turned light purple. After stirring at ambient temperature for 20 hours, the remaining starting material was consumed by adding boron tribromide (2.5 mL, 1 M solution in dichloromethane). The reaction was quenched with aqueous hydrochloric acid (1N, 20 mL) to afford a homogeneous mixture. The layers were separated and the aqueous layer washed with dichloromethane (20 mL). The pH of the aqueous layer was adjusted to 12 by addition of aqueous sodium hydroxide (50%) at which time a solid precipitated out of solution. The solid was stirred for 18 hours, collected by filtration and washed with water. The crude product was purified by chromatography over silica gel (eluting with CMA) to afford a white powder.
The powder was triturated with methanol (20 mL). The resulting solid was isolated by filtration, washed with methanol and dried for 4 hours at 65 C to provide 150 mg of {4-amino-1-[2,2-dimethyl-3-(methylsulfonyl)propyl]-1H-imidazo[4,5-c]quinolin-2-yl}methanol as a white powder, mp 23 0-232 C.
Anal. Calcd for C17H22N403S: %C, 56.33; %H, 6.12; %N, 15.46. Found: %C, 56.33;
%H, 6.31; %N, 15.27.

Example 144 N- { 2- [4-amino -2-(hydroxymethyl) -1 H-imi dazo [4, 5-c] quino l in-1-yl]
ethyl }-N-isopropylurea N NOH
N
\-~ N
H~ ~
O
A stirring solution of1V {2-[4-amino-2-(ethoxymethyl)-1.H-imidazo[4,5-c]quinolin-l-yl]ethyl}-N-isopropylurea (400 mg, 1.1 mmol) in dichloromethane (50 mL) was sealed with a septum and purged witli nitrogen gas. The solution was cooled in an ice/water bath and a 1.0 M solution of boron tribromide in dichloromethane (2.2 mL) was added via syringe. The resulting mixture was stirred for 2 hours while warming to ambient temperature. The mixture was cooled back to 0 C in an ice/water bath and the second portion of boron tribromide (1.0 M, 5.5 mL) was added. The reaction was stirred for 18 hours while warming to ambient temperature. Aqueous hydrochloric acid (6N, 10 ml) was added and the mixture was stirred for 1 hour. The layers were separated and the aqueous fraction was neutralized by the slow addition of solid sodiuni hydroxide until the pH reached 14. A fine precipitate formed. The aqueous mixture was extracted with chloroform (2x 50 mL) and filtered. The resulting solid (filter cake) was combined with the organic extracts, methanol (50 mL), and silica gel (5 g). The mixture was concentrated under reduced pressure. The crude product absorbed on silica was purified by chromatography using a HORIZON HPFC system (silica cartridge, eluting with 0-35%
CMA in chloroform over 2.6 L) followed by recrystallization from acetonitrile to provide 170 mg of N-{2-[4-amino-2-(hydroxymethyl)-1H-imidazo[4,5-c]quinolin-1-yl]ethyl}-IV'-isopropylurea as an off-white solid, mp >240 C.
1H NMR (500 MHz, DMSO-d6) 6 8.30 (d, J= 7.9 Hz, 1H), 7.61 (dd, J= 8.3, 0.9 Hz, 1H), 7.43 (m, 1H), 7.24 (m, 1H), 6,53 (br s, 2H), 5.99 (t, J= 5.8 Hz, 1H), 5.82 (d, J= 7.8 Hz, 1 H), 5.67 (d, J = 5.8 Hz, 1H),4.75(d,J=5.8Hz,2H),4.66(t,J=6.7Hz,2H),3.69(m, 1H), 3.48 (q, J= 6.4 Hz, 2H), 1.01 (d, J= 6.5 Hz, 6H);
MS (APCI) m/z 343 (M + H)+;
Anal. Calcd. for CI7H22N602: %C, 59.63; %H, 6.48; %N, 24.54. Found: %C, 59.64;
%H, 6.59; %N, 24.58.

Example 145 N- {4-[4-amino-2-(hydroxymethyl)-1 H-imidazo [4,5-c]quinolin-l-yl] butyl } cyclopentane carb oxamide N N OH
N

H

Boron tribromide (2.5 equivalents, 14.6 mL of 1 M solution in dichloromethane) was added dropwise to a cooled (ice bath) suspension of N-{4-[4-amino-2-(ethoxymethyl)-1FI-imidazo[4,5-c]quinolin-1-yl]butyl}cyclopentanecarboxamide (2.4 g, 5.8 mmol) in dichloromethane (25 mL). The reaction mixture was allowed to slowly warm to ambient temperature and then stirred for 6 days. Additional boron tribromide (5 equivalents, 29 mmol, 29 mL) was added and the reaction was stirred at ambient until starting material was consumed. The reaction was quenched slowly with methanol (100 mL) and then concentrated under reduced pressure. The residue was combined with 6 M
hydrochloric acid (100 mL), heated to 50 C, and stirred for 2 hours. The resulting solution was cooled (ice bath) and then free-based (pH 9) with the addition of 6 M aqueous sodium hydroxide.
A brown gummy solid formed in the basic aqueous solution. The aqueous liquid was decanted from the solid and acetonitrile was added (30 niL). A white precipitate formed and was isolated by filtration. The white precipitate was then triturated with hot acetonitrile, allowed to cool, isolated by filtration, washed with ether, and dried under vacuum to provide .N-{4-[4-amino-2-(hydroxymethyl)-1 -H-imidazo[4,5-c]quinolin-l-yl]butyl}cyclopentanecarboxamide (0.48 g) as a fine white solid, mp 183-186 C;
MS
(ESI) m1z 382 (M+H)+; Anal. Calcd for C21H27N$02: C, 65.35; H, 7.18; N, 18.14;
Found C, 65.06; H, 6.90; N, 18.13.

Example 146 N-[4-(4-amino-2-hydroxymethyl-lH-imidazo[4,5-c]quinolin-1-yl)butyl]isobutyranlide N N OH
N
N O
-~L
H

Boron tribromide (2.5 equivalents, 15.6 mL of 1 M solution in dichloromethane) was added dropwise to a cooled (ice bath) suspension of N-[4-(4-amino-2-ethoxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)butyl]isobutyramide (2.4 g, 6.2 mmol) in dichloromethane (25 mL). The reaction mixture was allowed to slowly warm to ambient temperature and then stirred for I day. Additional boron tribromide (5 equivalents, 31 mmol, 31 mL) was added to the mixture. The reaction was quenched slowly with methanol (100 mL) and then concentrated under reduced pressure. The residue was combined with 6 M hydrochloric acid (100 mL), heated to 50 C, and stirred for 2 hours.
The resulting solution was cooled (ice bath) and then free-based (pH 9) with the addition of 6 M sodium hydroxide. A brown gummy solid formed in the basic aqueous solution.
The resulting solid was extracted with dichloromethane (6 x 50 mL). The combined extracts were washed with brine (100 mL), dried with magnesium sulfate, filtered, and then concentrated under reduced pressure. This material was purified by prep HPLC
(Analogix Separation System, Biotage Si 40+M column, eluted with a gradient of 0-20%
methanol in dichloromethane with 1% ammonium hydroxide) to provide a light brown solid. The solid was triturated with hot acetonitrile, allowed to cool, isolated by filtration, washed with ether, and dried under vacuum to provide N-[4-(4-amino-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)butyl]isobutyramide (0.049 g) as a white solid, mp 222-224 C; MS (ESI) m/z 356 (M+H)+; Anal. Calcd for C19H25N502=0.25HBr=0.10H20: C, 60.46; H, 6.80; N, 18.55; Found C, 60.26; H, 6.64; N, 18.43.

Example 147 N- [4-(4-amino-2-hydroxymethyl-1 H-imidazo [4, 5 -c] quinolin-l-yl)butyl]methanesulfonamide N N OH
N

ot H-S

Boron tribromide (2.5 equivalents, 20 mL of 1 M solution in dichloromethane) was added dropwise to a cooled (ice bath) suspension ofN-[4-(4-amino-2-ethoxymethyl-lH-imidazo[4,5-c]quinolin-1-yl)butyl]methanesulfonamide (3g, 7.92 mmol) in dichloromethane (20 mL). The reaction mixture was allowed to slowly warin to ambient temperature and then stirred for 4 hours. Additional boron tribromide (2 mL) was added and the mixture was stirred for 3 hours. The reaction was quenched slowly with methanol (20 mL) and then concentrated under reduced pressure. The residue was combined with 6 M hydrochloric acid (5OmL), heated to 50 C, and stirred for 2 hours. The resulting solution was concentrated under reduced pressure to a slurry that cooled (ice bath) and then free-based with the addition of 71VI ammonia in methanol (40 mL). The mixture was concentrated under reduced pressure and the addition of 7 M ammonia in methanol (40mL) was repeated 2 more times. The concentrated brown sludge lilce material was purified by prep HPLC (ISCO Combiflash Separation System, Biotage Si 40+M
column, eluted with a gradient of methanol in dichloromethane with 1% ammonium hydroxide) to provide a light brown solid. The solid was triturated with hot acetonitrile, allowed to cool, isolated by filtration, washed with ether, and dried under vacuum to provide N-[4-(4-amino-2-hydroxymethyl-1H imidazo[4,5-c]quinolin-l-yl)butyl]methanesulfonamide (0.1 g) as a fine beige solid, mp 216-219 C; MS (ESI) m/z 364 (M+H)+; Anal. Calcd for C16H2IN5O3S: C, 52.88; H, 5.82; N, 19.27; Found C, 52.62; H, 5.71; N, 19.02.
Example 148 (4-Amino-I-{4-[(methylsulfonyl)amino]butyl}-1H-imidazo[4,5-c]quinolin-2-yl)methyl N-[(benzyloxy)carbonyl]-L-valinate --< ~-O
NH2 = H
N N O~
N~-J O
O, NH
S.
O
To a stirred suspension of N-[4-(4-amino-2-hydroxymethyl-lH-imidazo[4,5-c]quinolin-l-yl)butyl]methanesulfonamide (2.1 g, 5.8 inmol) in THF was added triphenylphosphine (1.5 equivalents, 8.7 mmol, 2.2 g) followed by CBZ-L-valine (1.5 equivalents, 8.7 mmol, 2.3 g). The suspension was stirred for 5 min after which it was cooled in an ice-bath. To this cooled reaction mixture diisopropyl azodicarboxylate (DIAD,1.8 equivalents, 10.4 mmol, 2.0 mL) was added and the reaction was warmed to room temperature and stirred overnight. The solvent was evaporated under reduced pressure and the crude solid was purified by prep HPLC (ISCO Combiflash Separation System, Biotage Si 40+M column, eluted with a gradient of 0-8% methanol in dichloromethane with 1 / ammonium hydroxide) to provide a solid. The solid was heated in diethyl ether and filtered to afford (4-amino-l-{4-[(methylsulfonyl)amino]butyl}-1HH
imidazo[4,5-c]quinolin-2-yl)methyllV-[(benzyloxy)carbonyl]-L-valinate (2 g) as a beige solid, mp 99-100 C; MS (ESI) rn/z 597 (M+H)+; Anal. Calcd for C29H36N606S: C, 58.37;
H, 6.08; N, 14.08; Found C, 57.98; H, 6.31; N, 13.82.
Example 149 (4-Amino-l-{4-[(methylsulfonyl)amino]butyl}-1H-imidazo[4,5-c]quinolin-2-yl)methyl L-valinate N N O
O
~
N
O. .NH
'S, / 'O

To a hydrogenation bottle was added (4-amino-l-{4-[(methylsulfonyl)amino]butyl } -1 H-imidazo [4,5-c]quinolin-2-yl)methyllV-[(benzyloxy)carbonyl]-L-valinate (1.5 g, 2.5 mmol) followed by a mixture of methanol (30 mL), THF (15 mL) and water (5 mL) and conc HC1(5 mL). To this was added Pd/C
(90 mg) and the reaction was hydrogenated at 40 psi (2.8 X 105 Pa) overnight.
To the reaction mixture was added conc. HCI (5 mL) and Pd/C (90 mg) and the reaction was hydrogenated at 40 psi (2.8 X 105 Pa) for 18 hours. The reaction was filtered through CELITE filter aid and the filtrate was evaporated to afford a clear oil. The product was isolated by prep HPLC (ISCO Combiflash Separation System, Biotage Si 40+M
column, eluted with a gradient of 0-8% methanol in dichloromethane with 1% ammonium hydroxide) to provide (4-amino-l-{4-[(methylsulfonyl)amino]butyl}-1H-imidazo[4,5-c]quinolin-2-yl)methyl L-valinate (0.495 g) as an off white solid, mp 161-163 C; MS
(ESI) tn/z 463 (M+H) "; Anal. Calcd for C21H30N6O4S: C, 54.53; H, 6.54; N, 18.17; Found C, 53.96; H, 6.62; N, 17.85, delta C = 0.57.
Example 150 [4-Amino-l-(tetrahydro-2H-pyran-4' ylmethyl)-1 H-imidazo[4,5-c]quinolin-2-yl]methanol N N OH
N

\'-C
Part A
Under a nitrogen atmosphere THF (90 mL) and triethylamine (17.5 mL, 125.6 mmol) were added sequentially to a mixture of crude 4-chloro-3 -nitroquinoline (13.10 g, 62.81 mmol) and 1-tetrahydro-2H-pyran-4-ylmethylamine hydrochloride (10.0 g, 65.95 mmol). The reaction mixture was placed in an oil bath at 45 C for 1 hour and then concentrated under reduced pressure. The residue was diluted with THF (30 mL) and water (200 mL). The THF was removed under reduced pressure. A solid was isolated by filtration and dried to provide 16.10 g of 3-nitro-N-(tetrahydro-2H-pyran-4-ylmethyl)quinolin-4-amine as a light yellow solid.
Part B

A mixture of 3-nitro-N-(tetrahydro-2H-pyran-4-ylmethyl)quinolin-4-amine (2.50 g), 10% palladium on carbon (0.25 g), and ethanol (40 mL) was placed under hydrogen pressure on a Parr apparatus. When the reaction was complete, the mixture was filtered through a layer of CELITE filter agent. The filter cake was washed with ethanol. The filtrate was concentrated under reduced pressure to provide 2.23 g of N4-(tetrahydro-2H-pyran-4-ylmethyl)quinoline-3,4-diamine as a yellowish-orange oil.
Part C

Chloroacetyl chloride (12 mL, 151 mmol) was dissolved in dichloromethane (30 mL) and added via addition funnel, over 20 minutes, to a stirring solution of (tetrahydro-2H-pyran-4-ylmethyl)quinoline-3,4-diamine (35.3g, 137 mmol) in dichloromethane (300 mL). The resulting solution was stirred at ambient temperature under nitrogen for 24 hours at which point the solution was heated to 40 C
for an additiona124 hours. The mixture was cooled to ambient temperature, diluted with dichloromethane (150 mL) and transferred to a separatory fu.nnel. The organic layer was washed with water (2 x 200 mL) and brine (2 x 200 mL), dried over magnesium sulfate, filtered and concentrated under reduced pressure to provide 38.3 g of 2-(chloromethyl)-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinoline as a light brown solid.
Part D

3-Chloroperoxybenzoic acid (mCPBA) (3.8 g of 77% pure material, 14.2 mmol) was added to a stirring solution of 2-(chloromethyl)-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinoline (3.0g, 9.50 mmol) in dichloromethane (60 mL). After 15.5 hours, ammonium hydroxide (12 mL) and then p-toluenesulfonyl chloride (2.2g, 11.4 mmol) were added to the stirring solution and the biphasic mixture was stirred at ambient temperature for 3 hours. The reaction was diluted with water (50 mL) and then transferred to a separatory funnel. The aqueous layer was extracted with dichloromethane (3 x 100 mL) and the combined organic fractions dried over magnesium sulfate, filtered and concentrated under reduced pressure. The residue was purified by column chromatography using a HORIZON HPFC system (silica cartridge, eluting with 3-20%
methanol in dichloromethane) to provide 1.6 g of 2-(chloromethyl)-1-(tetrahydro-2H=
pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinolin-4-amine as a yellow solid.
Part E
Potassium acetate (0.41 g, 4.16 mmol) and potassium iodide (0.28g, 1.66 mmol) were added to a stirring solution of 2-(cl-doromethyl)-1-(tetrahydro-2H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinolin-4-amine (0.55 g, 1.66 mmol) and the resulting suspension was heated to 50 C. After 17 hours, the suspension was cooled to ambient temperature and concentrated under reduced pressure. The residue was suspended in methanol (10 mL) and water (5 mL) and lithium hydroxide monohydrate (0.35 g, 8.31 mmol) was added in one portion. The resulting solution was stirred at ambient temperature 18 hours and concentrated under reduced pressure. The residue was diluted with water (20 mL) and neutralized with hydrochloric acid (6 N in water). The aqueous layer was extracted with dichloromethane (2 x 50 mL) and ethyl acetate (50 mL). The combined organic fractions were concentrated to a yellow solid which was crystallized from acetonitrile.
The crystals were isolated by filtration and dried in a vacuum oven at 65 C to provide 0.20 g of [4-amino-l-(tetrahydro-2.H-pyran-4-ylmethyl)-1H-imidazo[4,5-c]quinolin-2-yl]methanol as an off-white solid, mp 239-241 C.
Anal. calcd for C17H2oN~02-0.2H20: C, 64.62; H, 6.51; N, 17.73. Found: C, 64.45; H, 6.69; N, 17.62.

Examples 151 - 229 Part A
A solution of 1-(4-aminobutyl)-2-methoxymethyl-lH-imidazo[4,5-c]quinoline-4-amine (30 mg, 1 eq, prepared according to the general method of Example 3 using methoxyacetyl chloride in lieu of 3-methoxypropionyl chloride) and IiNIV-diisopropylethylamine (2 eq) in N,N-dimethylacetamide (1 mL) was added to a tube containing a reagent (1.1 eq) from the table below. The reaction mixture was vortexed overnight and then quenched with water (100 L). The solvents were removed by vacuum centrifugation. The residue was purified by solid-supported liquid-liquid extraction according to the following procedure. The sample was dissolved in chloroform (1 mL) then loaded onto diatomaceous earth that had been equilibrated with 1 M sodium hydroxide (600 L) for about 20 minutes. After 10 minutes chloroform (500 L) was added to elute the product from the diatomaceous earth into a well of a collection plate.
After an additional 10 minutes the process was repeated with additional chloroform (500 gL). The solvent was then removed by vacuum centrifugation.
Part B
The residue (in a test tube) was combined with dichloromethane (500 L) and the tube was vortexed to dissolve the solids. The solution was cooled (0 C) and then combined with boron tribromide (400 L of 1 M in dichloromethane). The mixture was vortexed for 5 minutes, chilled for 30 minutes, and then vortexed at ambient temperature for 64 hours. Additional dichloromethane (500 L) and boron tribromide (400 L
of 1 M
in dichloromethane) were added and the mixture was vortexed overnight. The solvent was then removed by vacuum centrifugation. The residue was diluted with methanol (500 L) and hydrochloric acid (500 L of 6 N). The solvents were removed by vacuum centrifugation. The compounds were purified according to the method described in Examples 8 - 72. The table below shows the reagent used for each example, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.
NHZ
N N OH
N

H'R
Example Reagent R Measured Mass (M+H) 151 None --H 286.1658 O
152 Cyclopropanecarbonyl 354.1907 chloride O
153 Methoxyacetyl chloride 344.1699 OH

O
154 Cyclobutanecarbonyl chloride 368.2050 O
155 Isovaleryl chloride CH3 370.2206 O

156 Pentanoyl chloride 370.2208 O

157 Benzoyl chloride 390.1909 ~s O
158 Cyclohexanecarbonyl 396.2412 chloride O
159 Cyclopentylacetyl 396.2411 --O
chloride O

404.2069 160 m-Toluoyl chloride ~CH3 161 o- Toluoyl chloride -. CH3 404.2072 ~ /

162 p- Toluoyl chloride Q 404.2108 O
163 Phenylacetyl chloride 404.2056 164 Dimethylaminoacetyl ~ CH3 371.2157 chloride hydrochloride N

165 2-Fluorobenzoyl chloride F 408.1819 O
166 3-Fluorobenzoyl chloride -- 408.1811 / F

167 4-Fluorobenzoyl chloride 408.1819 F
O

3-Cyanobenzoyl chloride 5.1847 415.1847 -- =N
O
169 Hydrocinnamoyl chloride 418.2200 ~

170 2-Methoxybenzoyl ~ OH 406.1880 chloride \
/

171 3-Methoxybenzoyl ~OH
406.1876 chloride 0 172 p-Anisoyl chloride 406.1860 OH

O
424.1517 173 3-Chlorobenzoyl chloride ~cl 174 4-Chlorobenzoyl chloride 424.1525 CI

175 Isonicotinoyl chloride 7- 391.1874 hydrochloride N
N

176 Nicotinoyl chloride 391.1895 hydrochloride N

177 Picolinoyl chloride 391.1846 hydrochloride ~JO
tyans-2-Phenyl-1-178 cyclopropanecarbonyl 430.2213 chloride .0 179 Methanesulfonyl chloride --S=CH3 364.1421 ~ O
180 Ethanesulfonyl chloride \- 378.1595 ~ ,O
1 -Propanesulfonyl S
181 chloride ~ 392.1753 o Dimethylsulfamoyl 182 S 393.1685 chloride .N-CH

0,0 183 1-Butanesulfonyl chloride 406.1881 p 0, __S!
184 Benzenesulfonyl chloride 426.1591 O
Sp i 185 1-Methylimidazole-4- ~ 430.1668 sulfonyl chloride N' ( N.CN

o p 2-Thiophenesulfonyl S
432,1135 186 chloride b S O
'"Sp , 187 3-Methylbenzenesulfonyl b chloride q Sp ' 188 o-Toluenesulfonyl \ 440.1758 chloride H3C ~
-_ ~ , 189 p-Toluenesulfonyl b 440.1766 chloride ~

O'"p 190 2-Fluerobenzenesulfonyl b 44 4.1479 chloride F 9S p , '~, 3-Fluorobenzenesulfonyl 444.1517 191 chloride ~ ~
~
F

d 192 4-Fluorobenzenesulfonyl 444.1496 chloride F
p o --s 193 3-Cyanobenzenesulfonyl 451.1568 chloride N
O ,p 194 4-Cyanobenzenesulfonyl qN 451.1 chloride N
0 ,O

195 beta-Styrenesulfonyl 452.1725 chloride O
-'Sp 42.1534 196 Methoxybenzenesulfonyl b chloride HO

197 Methoxybenzenesulfonyl 442.1557 chloride pH

198 2-Chlorobenzenesulfonyl b 460.1173 chloride GI O ,p 199 3-Chlorobenzenesulfonyl 460.1242 chloride ci O
A
-~S
200 4-Chlorobenzenesulfonyl 460.1191 chloride CI

201 3-Pyridinesulfonyl b 427.1530 chloride hydrochloride 00 3,4-202 Dimethoxybenzenesulfon 458.1452 yl chloride -_ HO OH

~S3,4-203 Dichlorobenzenesulfonyl 494.0806 chloride CI C) O
204 Methyl isocyanate 343.1862 O

205 Ethyl isocyanate N 357.2018 -~, 0 206 Isopropyl isocyanate N CH3 371.2181 ~
207 n-Propyl isocyanate H~ 371.2187 ~
208 n-Butyl isocyanate H 385.2314 O
209 Cyclopentyl isocyanate N--O 397.2312 H

~
210 Pentyl isocyanate H 399.2512 211 Phenyl isocyanate N 405.2047 H \ /

212 Cyclohexyl isocyanate N 411.2473 H~

213 2-Fluorophenyl N 423.1959 isocyanate H \ /
F
O

3-Fluorophenyl N
214 isocyanate H 423.1924 F

4-Cyanophenyl 430.1979 215 isocyanate H \ / = N

(R)-(+)-alpha- N , CH
216 Methylbenzyl isocyanate 433.2370 217 (~-(-)- alpha- N
433.2327 Methylbenzyl isocyanate O

433.2333 218 2-Phenylethylisocyanate N

219 2-Methoxyphenyl 421.2006 isocyanate H
HO
O
4-Methoxyphenyl 220 isocyanate H j\ 421.1958 ' OH

2-Chlorophenyl N
221 isocyanate H 439.1650 CI
O
4-Chlorophenyl 222 isocyanate H 439.1656 \~CI
trans-2- O
223 Phenylcyclopropyl 445.2328 isocyanate H
O
224 N,N-Dimethylcarbamoyl 357.2005 chloride H C.N-CH3 O
225 1 -Pyrrolidinecarbonyl N 383,2168 chloride 226 1 -Piperidinecarbonyl N 397.2329 chloride ' ~

/
4-Morpholinylcarbonyl N
227 chloride 399.2112 O
O
4-Methyl-l- N
228 Piperazinecarbonyl ~~ 412.2439 chloride N

O
229 N-Methyl-N- N ~ 419.2167 phenylcarbamoyl cliloride H3C
Examples 230 - 245 Part A
A solution of 1-(2-amino-2-methylpropyl)-2-methoxymethyl-lH-imidazo[4,5-c]quinoline-4-amine (31 mg, 1 eq, prepared according to the general method of Example 3 using methoxyacetyl chloride in lieu of 3-methoxypropionyl chloride and tert-butyl N{2-[(3-aminoquinolin-4-yl)amino]-1,1-dimethylethyl}carbamate in lieu of tert-butyl N-{4-[(3-aminoquinolin-4-yl)amino]butyl}carbamate) and N, N-diisopropylethylamine (2 eq) in N,N dimethylacetamide (1 mL) was placed in a test tube. A reagent (1.1 eq) from the table below was added and the reaction mixture was vortexed overnight. The reaction was quenched with concentrated ammonium hydroxide (100 L) and the solvents were removed by vacuum centrifugation.
Part B
The residue (in a test tube) was combined with dichloromethane (1 mL) and the tube was vortexed to dissolve the solids. The solution was cooled (0 C) and then combined with boron tribromide (400 L of 1 M in dichloromethane). The reaction was maintained at about 0 C for 20 minutes. Methanol (1 mL) and hydrochloric acid (500 L
of 6 N) were added and the tube was vortexed for about 30 minutes. The solvents were removed by vacuum centrifugation. The compounds were purified according to the method described in Examples 8 - 72. The table below shows the reagent used for each example, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

N NOH
~ NCH3 ~--CH3 H'R
T R Measured Mass Example Reagent (M+H) 230 None H 286.1687 231 Cyclopropanecarbonyl 354.1936 chloride 232 Butyryl chloride 356.2094 56.2119 233 Isobutyryl chloride ~CH3 3 HC O

234 Cyclopentanecarbonyl 382.2259 chloride 235 Benzoyl chloride 390.1908 O

236 Nicotinoyl chloride 391.1844 hydrochloride N

Methanesulfonyl 0 237 -'SOCH3 364.1414 chloride Op ~ S' 426.1617 238 Benchlorideonyl b 2,2- 0 '0 2, --s 239 Trifluoroethanesulfon ~F 432.1339 yl chloride F
F
O
p -'S

44.1523 240 Fluorobenzenesulfony b 1 chloride F

241 n-Propyl isocyanate N 371.2215 O
242 Cyclopentyl N 397.2327 isocyanate H--O
O
243 Phenyl isocyanate N '' 405.2063 H \ ~
O
244 Cyclohexyl isocyanate N 411.2515 H~
3-Fluorophenyl N
245 isocyanate H ~ ~ 423.1955 F
Examples 246 - 257 Part A
To a rotuid-bottomed flask containing 1-(4-aminobutyl)-2-methoxymethyl-lH-imidazo[4,5-c]quinolin-4-amine (10.0 g, 33.4 mmol) was added methanol (160 mL) followed by acetic acid (40 mL). The reaction was stirred for 5 minutes and pyridine 3-carboxaldehyde (5.4 g, 50.1 mmol) was added and the reaction was stirred overnight at ambient temperature. Sodium cyanoborohydride (1 M in THF, 33.4 mL, 33.4 mmol) was added to the resultant imine in portions over 10 minutes. After 45 minutes the solvent was evaporated to afford an oil. To the oil was added saturated aqueous sodium bicarbonate (200 mL) and the aqueous layer was washed with ethyl acetate (200 mL) and dichloromethane (200 mL). The product was extracted from the aqueous with 20%
methanol (2 x 100 mL) in dichloromethane. The organic layers were combined and the solvent evaporated to afford crude 2-methoxymethyl-1-{4-[(pyridin-3-ylmethyl)amino]butyl}-1.H-imidazo[4,5-c]quinolin-4-amine (about 2 g). The aqueous layer was again extracted with 20% dimethylformamide (2 x 100 mL) in dichloromethane.
The organic layers were combined and the solvent evaporated to afford crude 2-methoxymethyl-l- { 4- [(pyridin- 3-ylmethyl) amino] buty 1}-1 H-imi dazo [4, 5-c] quino lin-4-amine (about 2 g).

Part B
A solution of 2-methoxymethyl-l-{4-[(pyridin-3-ylmethyl)amino]butyl}-1H-imidazo[4,5-c]quinolin-4-amine (40 mg, 1 eq) and N,N-diisopropylethylamine (2 eq) in N,N-dimethylacetamide (1 mL) was added to a tube containing a reagent (1.1 eq) from the table below. The reaction mixture was vortexed for 4 hours and then quenched witli water (50 L). Tlie solvents were removed by vacuum centrifugation. The residue was purified by solid-supported liquid-liquid extraction according to the following procedure. The sample was dissolved in chloroform (1 mL) then loaded onto diatomaceous earth that had been equilibrated with 1 M sodium hydroxide (600 L) for about 20 minutes.
After 10 minutes chloroform (500 gL) was added to elute the product from the diatomaceous earth into a well of a collection plate. After an additional 10 minutes the process was repeated with additional chloroform (500 L). The solvent was then removed by vacuum centrifugation.
Part C
The residue (in a test tube) was combined with dichloromethane (500 L) and the tube was vortexed to dissolve the solids. The solution was cooled (0 C) and then combined with boron tribromide (400 L of I M in dichloromethane). The mixture was vortexed for 10 minutes, chilled for 30 minutes, and then vortexed at ambient temperature overnight. The solvent was then removed by vacuum centrifugation. The residue was diluted with methanol (500 L) and hydrochloric acid (500 L of 6 N) and the mixture was vortexed for about 30 minutes. The solvents were removed by vacuum centrifugation.
The compounds were purified according to the method described in Examples 8-72. The table below shows the reagent used for each example, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

N~ NOH
N
N
N
R
Example Reagent R Measured Mass (M+H) 246 None H 377.2087 O
247 Isobutyryl chloride CH 447.2468 O
248 Cyclohexanecarbonyl 487.2783 chloride 249 Phenylacetyl chloride 495.2465 250 4-Fluorobenzoyl 499 2272 chloride F
O
251 3-Methoxybenzoyl chloride 497.2263 HO
O
0-s 252 1-Methylimidazole-4- ~ 521.2071 sulfonyl chloride N' !
~N. CH

2,2,2- O s 253 Trifluoroethanesulfonyl 0 523.1717 chloride F
F F
O=S
alpha-Toluenesulfonyl 6---254 chloride 531.2134 0-'3-255 Methoxybenzenesulfon b 33.1941 yl chloride HO

256 Isopropyl isocyanate H C~NH 462.2611 O---~
3-Fluorophenyl NH
257 isocyanate 514.2357 F
Examples 258 - 322 The compounds in the table below were prepared and purified according to the methods of Parts B and C of Examples 246 - 257 using 1-(4-benzy)aminobutyl)-2-ethoxymethy-lH-imidazo[4,5-c]quinolin-4-amine in lieu of 2-methoxymethyl-l-{4-[(pyridin-3 -ylmethyl)amino]butyl }-1 H-imidazo [4, 5-c]quinolin-4-amine. 1-(4-Benzylaminobutyl)-2-ethoxymethy-IH-imidazo[4,5-c]quinolin-4-amine was prepared according to the general method of Part A of Examples 246 - 257 using benzaldehyde in lieu of pyridine 3-carboxaldehyde and 1-(4-aminobutyl)-2-ethoxymethyl-lH-imidazo[4,5-c]quinolin-4-amine in lieu of 1-(4-aminobutyl)-2-methoxymethyl-lH-imidazo[4,5-c]quinolin-4-amine. The table below shows the reagent used for each example, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

NHz N N OH
N

RN
Example Reagent R Measured Mass (M+I~
Cyclobutanecarbonyl 258 chloride 458.2550 O
259 DL-2-Methylbutyryl CH3 460.2707 chloride O
260 Isovaleryl chloride H C 460.2714 O

261 Pentanoyl chloride 460.2730 O
262 Pivaloyl chloride CH3 460.2714 O
263 Cyclopentanecarbonyl 472.2712 chloride O
264 tert-Butylacetyl chloride H C 474.2879 O
265 Benzoyl chloride 480.2398 O
266 Thiophene-2-carbonyl , 486.1971 chloride S
O
267 Cyclohexanecarbonyl 486.2893 chloride 268 Cyclopentylacetyl 486.2818 chloride 269 m-Toluoyl chloride / \ 494.2577 H3Ci 270 o-Toluoyl chloride H C 494.2531 271 p-Toluoyl chloride 494.2527 272 3-Fluorobenzoyl chloride 498.2307 F

273 4-Fluorobenzoylchloride / \ 498.2326 F

274 3-Cyanobenzoyl chloride 505.2378 N o, O
275 4-Cyanobenzoyl chloride 505.2387 N
O
276 Hydrocinnamoyl chloride 508.2715 O
277 2-Methoxybenzoyl 496,2311 chloride HO /
O
278 3-Methoxybenzoyl 496.2314 chloride HO
O
279 p-Anisoyl chloride 496.2365 OH
O

280 3-Chlorobenzoyl chloride 514.2026 Cl O
281 4-Chlorobenzoyl chloride 514.2041 CI

O
282 Picolinoyl chloride 481.2361 hydrochloride N \

tr=ans-2-Phenyl-l- O
520.2695 283 cyclopropanecarbonyl uz~~J
chloride O

benzoyl 523.2802 284 4-Dimet chloride OZ:S~
285 1-Propanesulfonyl 482.2232 chloride 286 Dimethylsulfamoyl 483.2196 chloride N-CH
H3~i O
0=g/
287 2-Thiophenesulfonyl 522.1613 chloride S

Q, alpha-Toluenesulfonyl _ O=S~
288 chloride ? 530.2239 \
O
0=g 289 o-Toluenesulfonyl \ 530.2197 chloride H3C /

0=S
290 4-Fluorobenzenesulfonyl 534.2028 chloride F
O
o;,ss 291 3,5-Dimethylisoxazole-4- CH3 535.2106 sulfonyl chloride H3 ~ l N
O' O
O=S
292 2-Cyanobenzenesulfonyl 541.1968 chloride O S
293 3-Cyanobenzenesulfonyl /
chloride 541.2035 N

O, O=S~
294 beta-Styrene chloride sulfonyl c 42.2234 O

3- O:S~

32.2052 295 Methoxybenzenesulfonyl b chloride HO

/

296 Methoxybenzenesulfonyl 532.2037 chloride OH
O S
297 3-Pyridine sulfonyl 517.2015 chloride hydrochloride / ~
N-O
O=g~
2,5- OH
298 Dimethoxybenzenesulfon 548.1964 yl chloride HO
O
O_S
2,3-299 Dichlorobenzenesulfonyl CI 584.1294 chloride ci O/
0=g 3,5-300 Dichlorobenzenesulfonyl 584.1282 chloride CI
ci 301 Methyl isocyanate NH 433.2361 O_~/
302 Ethyl isocyanate NH 447.2538 \CH3 O
303 Isopropyl isocyanate H C~NH 461.2663 O

304 n-Propyl isocyanate NH 461.2691 NH
305 n-Butyl isocyanate 475.2860 306 sec-Butyl isocyanate H3Cl NH 475.2849 H3C'' NH
307 Pentyl isocyanate 489.3005 'Ir 308 Phenyl isocyanate a N 495.2511 H

309 Cyclohexyl isocyanate N 501.2978 a H
O
N
310 Benzyl isocyanate H 509.2675 311 3-Fluorophenyl N 513.2467 isocyanate H
F

4-Fluorophenyl 312 aisocyanate F ~ 513.2388 313 Cycloheptyl isocyanate H 515.3081 N
Cyclohexanemethyl 6 H
314 isocyanate 515.3163 315 4-Cyanophenyl N 520.2483 isocyanate N= H

3 4-Dimethylphenyl ' N
316 ~ isocyanate H3C \~ H 523.2786 o (S)-(-)-alpha- H3C õ N
~ 523.2786 317 Methylbenzyl isocyanate O~
2-Methylbenzyl N
318 isocyanate H3C 523.2860 N,N-Dimethylcarbamoyl 0 Z-~ 319 chloride H C.N_CH 447.2511 O~
320 Diethylcarbamyl chloride N-1 475.2828 321 1 -Piperidinecarbonyl N 487.2839 chloride O

322 N(4-Chlorobutyl)-N- 523.2588 methylcarbamyl chloride CI
Examples 323 - 329 The compounds in the table below were prepared according to the general method of Examples 111 - 140. The table shows a reference for the ether starting material, the structure of the resulting compound, and the observed accurate mass for the isolated trifluoroacetate salt.

N N
N~R2 Exampl Reference Measured e (ether) Rl R2 Mass (M+H) 323 U.S. Patent No. ~, OH 335.1158 6,667,312* H3C,S% a -~

U.S. Patent No. CH 336.1098 CH ~
324 6,677,349* H-S 3 U.S Patent No. N OH
325 364.1454 6,677,349* 0=S

U.S. Patent No.
OH 380.1391 326 6,677,347 0 ~H
Example 57 N
SO
H3C p O
327 U.S. Patent No. H ~-OH 444.0999 6,756,382*
c-ci U.S. Patent No.
328 6,683,088 0 ~H _7-OH 394.1588 Example 1 N
S=0 U.S. Patent No.
329 6,677,349 p O ~-OH 496.2401 Example 242 N-S

*Although not specifically exemplified, the compound is readily prepared using the disclosed synthetic methods.

Exemplary Compounds Certain exemplary compounds, including some of those described above in the Examples, have the following Formula lb and the following substituents n and Rl wherein each line of the table is matched to Formula Ib to represent a specific embodiment of the invention.

~
N N
\>--(CH2),,OH
N
Ri Ib n R1 1 2-[(cyclohexylcarbonyl)amino]-2-methylpropyl 1 2-[(cyclopropylcarbonyl)amino]ethyl 1 4-[(cyclopropylcarbonyl)amino]butyl 1 2- { [(1 -methylethyl)carbonyl]amino } ethyl 1 4-{ [(1-methylethyl)carbonyl]amino}butyl 1 2,2-dimethyl-3-(methylsulfbnyl)propyl 1 2-methyl-2-( { [(1-methylethyl)amino] carbonyl } amino)propyl 1 2-methyl-2-[(methylsulfonyl)amino]propyl I 4-[(methylsulfonyl)amino]butyl 1 2-[(methylsulfonyl)amino]ethyl 1 4-[(4-morpholinecarbonyl)amino]butyl 1 2-[(4-morpholinecarbonyl)amino]ethyl 1 tetrahydro-2H-pyran-4-ylmethyl 2 2-[(cyclohexylcarbonyl)amino]-2-methylpropyl 2 2-[(cyclopropylcarbonyl)amino]ethyl 2 4-[(cyclopropylcarbonyl)amino]butyl 2 2- { [(1-methylethyl)carbonyl] amino) ethyl 2 4- { [(1-methylethyl)carbonyl]amino}butyl 2 2,2-dimethyl-3-(methylsulfonyl)propyl 2 2-methyl-2-({[(1-methylethyl)amino]carbonyl}amino)propyl 2 2-methyl-2-[(methylsulfonyl)amino]propyl 2 4-[(methylsulfonyl)amino]butyl 2 2-[(methylsulfonyl)amino]ethyl 2 4-[(4-morpholinecarbonyl)amino]butyl 2 2-[(4-morpholinecarbonyl)amino]ethyl 2 tetrahydro-2H-pyran-4-ylmethyl CYTOKINE INDUCTION IN HUMAN CELLS
An in vitro human blood cell system is used to assess cytokine induction.
Activity is based on the measurement of interferon (a) and tumor necrosis factor (a) (IFN-a and TNF-a, respectively) secreted into culture media as described by Testerman et.
al. in "Cytokine Induction by the Immunomodulators Imiquimod and S-27609", Journal of Leukocyte Biology, 58, 365-372 (September, 1995).

Blood Cell Preparation for Culture Whole blood from healthy human donors is collected by venipuncture into vacutainer tubes or syringes containing EDTA. Peripheral blood mononuclear cells (PBMC) are separated from whole blood by density gradient centrifugation using HISTOPAQUE-1077 (Sigma, St. Louis, MO) or Ficoll-Paque Plus (Amersham Biosciences Piscataway, NJ). Blood is diluted 1:1 with Dulbecco's Phosphate Buffered Saline (DPBS) or Hank's Balanced Salts Solution (HBSS). Alternately, whole blood is placed in Accuspin (Sigma) or LeucoSep (Greiner Bio-One, Inc., Longwood, FL) centrifuge frit tubes containing density gradient medium. The PBMC layer is collected and washed twice with DPBS or HBSS and re-suspended at 4 x 106 cells/mL in RPMI
complete. The PBMC suspension is added to 96 well flat bottom sterile tissue culture plates containing an equal volume of RPMI complete media containing test compound.
Compound Preparation The compounds are solubilized in dimethyl sulfoxide (DMSO). The DMSO
concentration should not exceed a final concentration of 1% for addition to the culture wells. The compounds are generally tested at concentrations ranging from 30-0.014 M.
Controls include cell samples with media only, cell samples with DMSO only (no compound), and cell samples with reference compound.

Incubation The solution of test compound is added at 60 M to the first well containing RPMI
complete and serial 3 fold dilutions are made in the wells. The PBMC
suspension is then added to the wells in an equal volume, bringing the test compound concentrations to the desired range (usually 30-0.014 M). The final concentration of PBMC
suspension is 2 x 106 cells/mL. The plates are covered with sterile plastic lids, mixed gently and then incubated for 18 to 24 hours at 37 C in a 5% carbon dioxide atmosphere.

Separation Following incubation the plates are centrifuged for 10 minutes at 1000 rpm (approximately 200 x g) at 4 C. The cell-free culture supernatant is removed and transferred to sterile polypropylene tubes. Samples are maintained at -30 to -70 C until analysis. The samples are analyzed for IFN-a by ELISA and for TNF-a by IGEN/BioVeris Assay.
Interferon (a) and Tumor Necrosis Factor (a) Analysis IFN-a concentration is determined with a human multi-subtype colorimetric sandwich ELISA (Catalog Number 41105) from PBL Biomedical Laboratories, Piscataway, NJ. Results are expressed in pg/mL.
The TNF-a concentration is determined by ORIGEN M-Series Immunoassay and read on an IGEN M-8 analyzer from BioVeris Corporation, formerly known as IGEN
International, Gaithersburg, MD. The immunoassay uses a human TNF-a capture and detection antibody pair (Catalog Numbers AHC3419 and AHC3712) from Biosource International, Camarillo, CA. Results are expressed in pg/mL.
Assay Data and Analysis In total, the data output of the assay consists of concentration values of TNF-a and IFN-a (y-axis) as a function of compound concentration (x-axis).
Analysis of the data has two steps. First, the greater of the mean DMSO (DMSO
control wells) or the experimental background (usually 20 pg/mL for IFN-a and 40 pg/mL
for TNF-a) is subtracted from each reading. If any negative values result from background subtraction, the reading is reported as and is noted as not reliably detectable, In subsequent calculations and statistics, "*", is treated as a zero. Second, all background subtracted values are multiplied by a single adjustment ratio to decrease experiment to experiment variability. The adjustment ratio is the area of the reference compound in the new experiment divided by the expected area of the reference compound based on the past 61 experiments (unadjusted readings). This results in the scaling of the reading (y-axis) for the new data without changing the shape of the dose-response curve.
The reference compound used is 2-[4-amino-2-ethoxymethyl-6,7,8,9-tetrahydro-a,a-dimethyl-lH-imidazo[4,5-c]quinolin-1-yl]ethanol hydrate (U.S. PatentNo.
5,352,784;
Example 91) and the expected area is the sum of the median dose values from the past 61 experiments.
The minimum effective concentration is calculated based on the background-subtracted, reference-adjusted results for a given experiment and compound.
The minimum effective concentration ( molar) is the lowest of the tested compound concentrations that induces a response over a fixed cytokine concentration for the tested cytokine (usually 20 pg/mL for IFN-a and 40 pg/mL for TNF-a). The maximal response (pg/mL) is the maximal response attained in the dose response curve.

Compounds of the invention and close analogs were tested for their ability to induce cytokine biosynthesis using the test method described above. The analogs used are shown in the table below.

Analog Chemical Name Reference 1 N-[2-(4-Amino-2-methyl-lH-imidazo[4,5-c]quinolin-l- U.S. Patent 6,677,349 1)-1,1-dimethylethyl]methanesulfonamide 2 N-[2-(4-Amino-2-ethyl-lH-imidazo[4,5-c]quinolin-l- U.S. Patent 6,677,349 yl)- 1, 1 -dimethylethyl]methanesulfonamide 3 N-[2-(4-Amino-2-propyl-lH-imidazo[4,5-c]quinolin-l- U.S. Patent 6,677,349 yl)- 1, 1 -dimethylethyl]methanesulfonamide 4 N-[2-(4-Amino-2-ethoxymethyl-lFl-imidazo[4,5- U.S. Patent 6,677,349 c]quinolin-l-yl)-1,1-dimethylethyl]methanesulfonamide Example 268 5 N-{2-[4-Amino-2-(2-methoxyethyl)-1H-imidazo[4,5- Example 6 Part D
c]quinolin-1-yl]-l,1-dimethylethyl } methanesulfonamide This compound is not specifically exemplified but can be readily prepare using the synthetic methods disclosed in the cited reference The compounds of Examples 6 and 7 and several closely related analogs were tested using the test method described above. The IFN-a dose response curves for Example 6, Analog 2, Analog 3 and Analog 5 are shown in Figure 1. The TNF-a dose response curves for Example 6, Analog 2, Analog 3 and Analog 5 are shown in Figure 2.
The IFN-a dose response curves for Example 7, Analog 1, Analog 2 and Analog 4 are shown in Figure 3. The TNF-a dose response curves for Example 7, Analog 1, Analog 2 and Analog 4 are shown in Figure 4. The minimum effective concentration for the induction of IFN-a, minimum effective concentration for the induction of TNF-a, the maximal response for IFN-a, and the maximal response for TNF-a are shown in Table 5 below where # is the number of separate experiments in which the compound was tested.
When a compound was tested in more than one experiment the values shown are the median values.
Table 5 NNZ
N N

~
NH
Q O

Minimum Effective Maximal Response Compound R2 Concentration ( M) (p /mL) IFN TNF IFN TNF
Example 7-CH20H 3.330 30.00 2250 121 5 Example 6-(CH2)20H 1.11 >30 7521 * 3 Analog 1 -CH3 0.370 3.330 1846 1518 7 Analog 2 -CH2CH3 0.120 1.110 831 3670 4 Analog 3 -(CH2)2CH3 0.120 0.370 832 7245 9 Analog 4 -CHaOCH2CH3 0.040 0.370 889 10125 22 Analog 5 -(CH2)20CH3 0.014 0.12 825 12518 6 *TNF below experimental background of 40 pg/mL.
Compounds of the invention and close analogs were tested for their ability to induce cytokine biosynthesis using the test method described above. The minimum effective concentration for the induction of IFN-a, minimum effective concentration for the induction of TNF-a, the maximal response for IFN-a, and the maximal response for TNF-a are shown in Table 6 below where # is the number of separate experiments in which the compound was tested. When a compound was tested in more than one experiment the values shown are the median values.

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n o ir, The complete disclosures of the patents, patent documents, and publications cited herein are incorporated by reference in their entirety as if each were individually incorporated. Various modifications and alterations to this invention will become apparent to those skilled in the art without departing from the scope and spirit of this invention. It should be understood that this invention is not intended to be unduly limited by the illustrative embodiments and examples set forth herein and that such examples and enibodiments are presented by way of example only with the scope of the invention 1Q intended to be limited only by the claims set forth herein as follows.

Claims (20)

1. A compound of the Formula I:

wherein:
m is 0 or 1;
n is 1 or 2;
R is selected from the group consisting of C1-10 alkyl, C1-10 alkoxy, halogen, and C1-10 haloalkyl;
R1 is selected from the group consisting of:
-X-Y-R4, -X-R5, and -X-Het;
X is straight chain or branched chain alkylene optionally interrupted by one -O-group;
Y is selected from the group consisting of -S(O)0-2-and -N(R8)-Q-;
R4 is selected from the group consisting of hydrogen, alkyl, alkenyl, aryl, arylalkylenyl, heteroaryl, and heteroarylalkylenyl, wherein the alkyl, alkenyl, aryl, arylalkylenyl, heteroaryl, and heteroarylalkylenyl, groups can be unsubstituted or substituted by one or more substituents independently selected from the group consisting of alkyl, alkoxy, hydroxyalkyl, haloalkyl, haloalkoxy, halogen, nitro, hydroxy, mercapto, cyano, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocyclyl, amino, alkylamino, dialkylamino, and in the case of alkyl and alkenyl, oxo;
R5 is selected from the group consisting of:

Het is selected from the group consisting of tetrahydropyranyl and tetrahydrofuranyl;
R6 is selected from the group consisting of =O and =S;
R7 is C2-7 alkylene;
R8 is selected from the group consisting of hydrogen, alkyl, alkoxyalkylenyl, hydroxyalkylenyl, arylalkylenyl, and heteroarylalkylenyl;
R10 is C3-8 alkylene;

A is selected from the group consisting of -O-, -C(O)-, -CH2-, -S(O)0-2-, and -N(Q-R4)-;
Q is selected from the group consisting of a bond, -C(R6)-, -S(O)2, -C(R6)-N(R8)-, -S(O)2-N(R8)-, -C(R6)-O-, and -C(R6)-S-; and a and b are independently integers from 1 to 6 with the proviso that a + b is <= 7;
with the proviso that when Y is -S(O)0-2- then X can not contain an -O- group;

or a pharmaceutically acceptable salt thereof.

2. A compound of the Formula II:

wherein:
G1 is selected from the group consisting of:
-C(O)-R', .alpha.-aminoacyl, .ALPHA.-aminoacyl-.ALPHA.-aminoacyl, -C(O)-O-R', -C(O)-N(R")R', -C(=NY')-R', -CH(OH)-C(O)-OY', -CH(OC1-4alkyl)Y0, -CH2Y1, and -CH(CH3)Y1;
R' and R" are independently selected from the group consisting of C1-10 alkyl, C3-7 cycloalkyl, phenyl, benzyl, and 2-phenylethyl, each of which may be unsubstituted or substituted by one or more substituents independently selected from the group consisting of halogen, hydroxy, nitro, cyano, carboxy, C1-6 alkyl, C1-4 alkoxy, aryl, heteroaryl, aryl-C1-4 alkylenyl, heteroaryl-C1-4 alkylenyl, halo-C1-4 alkylenyl, halo-C1-4 alkoxy, -O-C(O)-CH3, -C(O)-O-CH3, -C(O)-NH2, -O-CH2-C(O)-NH2, -NH2, and -S(O)2-NH2, with the proviso that R" can also be hydrogen;
.alpha.-aminoacyl is an .alpha.-aminoacyl group derived from an amino acid selected from the group consisting of racemic, D-, and L-amino acids;
Y' is selected from the group consisting of hydrogen, C1-6 alkyl, and benzyl;
Y0 is selected from the group consisting of C1-6 alkyl, carboxy-C1-6 alkylenyl, amino-C1-4 alkylenyl, mono-N-C1-6 alkylamino-C1-4 alkylenyl, and di-N,N-C1-6 alkylamino-C1-4 alkylenyl;
Y1 is selected from the group consisting of mono-N-C1-6 alkylamino, di-N,N-C1-6 alkylamino, morpholin-4-yl, piperidin-1-yl, pyrrolidin-1-yl, and 4-C1-4 alkylpiperazin-1-yl;
m is 0 or 1;
n is 1 or 2;

R is selected from the group consisting of C1-10 alkyl, C1-10 alkoxy, halogen, and C1-10 haloalkyl;

R1 is selected from the group consisting of:
-X-Y-R4, -X-R5, and -X-Het;

X is straight chain or branched chain alkylene optionally interrupted by one -O-group;
Y is selected from the group consisting of -S(O)0-2-and -N(R8)-Q-;
R4 is selected from the group consisting of hydrogen, alkyl, alkenyl, aryl, arylalkylenyl, heteroaryl, and heteroarylalkylenyl, wherein the alkyl, alkenyl, aryl, arylalkylenyl, heteroaryl, and heteroarylalkylenyl, groups can be unsubstituted or substituted by one or more substituents independently selected from the group consisting of alkyl, alkoxy, hydroxyalkyl, haloalkyl, haloalkoxy, halogen, nitro, hydroxy, mercapto, cyano, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocyclyl, amino, alkylamino, dialkylamino, and in the case of alkyl and alkenyl, oxo;
R5 is selected from the group consisting of:

Het is selected from the group consisting of tetrahydropyranyl and tetrahydrofuranyl;
R6 is selected from the group consisting of =O and =S;
R7 is C2-7 alkylene;
R8 is selected from the group consisting of hydrogen, alkyl, alkoxyalkylenyl, hydroxyalkylenyl, arylalkylenyl, and heteroarylalkylenyl;
R10 is C3-8 alkylene;
A is selected from the group consisting of -O-, -C(O)-, -CH2-, -S(O)0-2-, and -N(Q-R4)-;
Q is selected from the group consisting of a bond, -C(R6)-, -S(O)2, -C(R6)-N(R8)-, -S(O)2-N(R8)-, -C(R6)-O-, and -C(R6)-S-; and a and b are independently integers from 1 to 6 with the proviso that a + b is <= 7;
with the proviso that when Y is -S(O)0-2- then X can not contain an -O- group;

or a pharmaceutically acceptable salt thereof.

3. A compound of the Formula III:

wherein:
G2 is selected from the group consisting of:
-X2-C(O)-R', .alpha.-aminoacyl, .alpha.-aminoacyl-.alpha.-aminoacyl, -X2-C(O)-O-R', -C(O)-N(R")R', and -S(O)2-R';
X2 is selected from the group consisting of a bond; -CH2-O-; -CH(CH3)-O-;
-C(CH3)2-O-; and, in the case of -X2-C(O)-O-R', -CH2-NH-;
R' and R" are independently selected from the group consisting of C1-10 alkyl, C3-7 cycloalkyl, phenyl, benzyl, and 2-phenylethyl, each of which may be unsubstituted or substituted by one or more substituents independently selected from the group consisting of halogen, hydroxy, nitro, cyano, carboxy, C1-6 alkyl, C1-4 alkoxy, aryl, heteroaryl, aryl-C1-4 alkylenyl, heteroaryl-C1-4 alkylenyl, halo-C1-4 alkylenyl, halo-C1-4 alkoxy, -O-C(O)-CH3, -C(O)-O-CH3, -C(O)-NH2, -O-CH2-C(O)-NH2, -NH2, and -S(O)2-NH2, with the proviso that R" can also be hydrogen;
.alpha.-aminoacyl is an .alpha.-aminoacyl group derived from an .alpha.-amino acid selected from the group consisting of racemic, D-, and L-amino acids;
m is 0 or 1;
n is 1 or 2;
R is selected from the group consisting of C1-10 alkyl, C1-10 alkoxy, halogen, and C1-10 haloalkyl;
R1 is selected from the group consisting of:
-X-Y-R4, -X-R5, and -X-Het;
X is straight chain or branched chain alkylene optionally interrupted by one -O-group;
Y is selected from the group consisting of -S(O)0-2-and -N(R8)-Q-;
R4 is selected from the group consisting of hydrogen, alkyl, alkenyl, aryl, arylalkylenyl, heteroaryl, and heteroarylalkylenyl, wherein the alkyl, alkenyl, aryl, arylalkylenyl, heteroaryl, and heteroarylalkylenyl, groups can be unsubstituted or substituted by one or more substituents independently selected from the group consisting of alkyl, alkoxy, hydroxyalkyl, haloalkyl, haloalkoxy, halogen, nitro, hydroxy, mercapto, cyano, aryl, aryloxy, heteroaryl, heteroaryloxy, heterocyclyl, amino, alkylamino, dialkylamino, and in the case of alkyl and alkenyl, oxo;
R5 is selected from the group consisting of:

Het is selected from the group consisting of tetrahydropyranyl and tetrahydrofuranyl;
R6 is selected from the group consisting of =O and =S;
R7 is C2-7 alkylene;
R8 is selected from the group consisting of hydrogen, alkyl, alkoxyalkylenyl, hydroxyalkylenyl, arylalkylenyl, and heteroarylalkylenyl;
R10 is C3-8 alkylene;
A is selected from the group consisting of -O-, -C(O)-, -CH2-, -S(O)0-2-, and -N(Q-R4)-;
Q is selected from the group consisting of a bond, -C(R6)-, -S(O)2, -C(R6)-N(R8)-, -S(O)2-N(R8)-, -C(R6)-O-, and -C(R6)-S-; and a and b are independently integers from 1 to 6 with the proviso that a + b is <= 7;
with the proviso that when Y is -S(O)0-2- then X can not contain an -O- group;

or a pharmaceutically acceptable salt thereof.

4. The compound or salt of any one of claims 1, 2, and 3, wherein n is 1.

5. The compound or salt of any one of claims 1, 2, and 3 wherein n is 2.

6. The compound or salt of any one of claims 1 through 5 wherein m is 0.

7. The compound or salt of any one claims 1 through 6 wherein R1 is -X-Y-R4 wherein X is straight chain or branched chain C1-6 alkylene which may be interrupted by one -O- group; Y is selected from the group consisting of -N(R8)-C(O)-, -N(R8)-S(O)2-, -N(R8)-C(O)-N(R8)-, and -S(O)2- wherein R8 is selected from hydrogen and methyl; and R4 is selected from the group consisting of C1-6 alkyl, isoquinolinyl, N-methylimidazolyl, pyridinyl, quinolinyl, phenyl, and phenyl substituted by a substituent selected from the group consisting of chloro, cyano, fluoro, hydroxy, and methyl.

8. The compound or salt of any one of claims 1 through 7 wherein R1 is selected from the group consisting of 2-[(cyclopropylcarbonyl)amino]ethyl, 4-[(cyclopropylcarbonyl)amino]butyl, 2-[(cyclohexylcarbonyl)amino]-2-methylpropyl, 2-{[(1-methylethyl)carbonyl]amino}ethyl, 4-{[(1-methylethyl)carbonyl]amino}butyl, 2-methyl-2-{[(1-methylethyl)carbonyl]amino}propyl, 2-[(methylsulfonyl)amino]ethyl, 4-[(methylsulfonyl)amino]butyl, 2-methyl-2-[(methylsulfonyl)amino]propyl, 2-methyl-2-({[(1-methylethyl)amino]carbonyl}amino)propyl, and 2,2-dimethyl-3-(methylsulfonyl)propyl.

9. The compound or salt of any one claims 1 through 6 wherein R1 is -X-Y-R4 wherein X is straight chain or branched chain C1-8 alkylene which may be interrupted by one -O- group; Y is selected from the group consisting of -N(R8)-C(O)-, N(R8)-S(O)2-, -N(R8)-C(O)-N(R8a)-, and -S(O)2- wherein R8 is hydrogen, methyl, benzyl, or pyridin-3-ylmethyl; R8a is hydrogen, methyl, or ethyl, and R4 is selected from the group consisting of C1-7 alkyl, haloC1-4 alkyl, hydroxyC1-4 alkyl, phenyl, benzyl, 1-phenylethyl, 2-phenylethyl, 2-phenylethenyl, phenylcyclopropyl, pyridinyl, thienyl, N-methylimidazolyl, 3,5-dimethylisoxazolyl, wherein benzyl is unsubstituted or substituted by a methyl group, and phenyl is unsubstituted or substituted by one or two substituents independently selected from the group consisting of methyl, fluoro, chloro, cyano, hydroxy, and dimethylamino.

10. The compound or salt of any one of claims 1 through 6 wherein R1 is -X-R5 wherein X is C1-6 alkylene, and R5 is

11. The compound or salt of any one of claims 1 through 6 or 10 wherein R1 is selected from the group consisting of 4-(1,1-dioxidoisothiazolidin-2-yl)butyl, 4-[(4-morpholinecarbonyl)amino]butyl, and 2- [(4-morpholinecarbonyl)amino] ethyl.

12. The compound or salt of any one of claims 1 through 6 wherein R1 is -C1-4 alkylenyl-Het.

13. The compound or salt of any one of claims 1 through 6 or 12 wherein R1 is tetrahydro-2H-pyran-4-ylmethyl.

14. A compound selected from the group consisting of N-[4-(4-amino-2-hydroxymethyl-1H-imidazo[4,5-c]quinolin-1-yl)butyl]methanesulfonamide and N-{4-[4-amino-2-(2-hydroxyethyl)-1H-imidazo[4,5-c]quinolin-l-yl]butyl]}methanesulfonamide, or a pharmaceutically acceptable salt thereof.

15. N-{2-[4-Amino-2-(hydroxymethyl)-1H-imidazo[4,5-c]quinolin-1-yl]-1,1-dimethylethyl}methanesulfonamide or a pharmaceutically acceptable salt thereof.

16. A pharmaceutical composition comprising a therapeutically effective amount of a compound or salt of any one of claims 1 through 15 and a pharmaceutically acceptable carrier.

17. A method of preferentially inducing the biosynthesis of IFN-.alpha. in an animal comprising administering an effective amount of a compound or salt of any one of claims 1 through 15 or a pharmaceutical composition of claim 16 to the animal.

18. A method of treating a viral disease in an animal in need thereof comprising administering a therapeutically effective amount of a compound or salt of any one of claims 1 through 15 or the pharmaceutical composition of claim 16 to the animal.

19. A method of treating a neoplastic disease in an animal in need thereof comprising administering a therapeutically effective amount of a compound or salt of any one of claims 1 through 15 or the pharmaceutical composition of claim 16 to the animal.

20. The method of any one of claims 17, 18, or 19 wherein the compound or salt or pharmaceutical composition is administered systemically.