CA2637669A1 - Simultaneous aspirator and dispenser for multiwell plates and similar devices - Google Patents
Simultaneous aspirator and dispenser for multiwell plates and similar devices Download PDFInfo
- Publication number
- CA2637669A1 CA2637669A1 CA002637669A CA2637669A CA2637669A1 CA 2637669 A1 CA2637669 A1 CA 2637669A1 CA 002637669 A CA002637669 A CA 002637669A CA 2637669 A CA2637669 A CA 2637669A CA 2637669 A1 CA2637669 A1 CA 2637669A1
- Authority
- CA
- Canada
- Prior art keywords
- sample
- aspirating
- dispensing
- testing device
- well
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1065—Multiple transfer devices
- G01N35/1074—Multiple transfer devices arranged in a two-dimensional array
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
- G01N35/1011—Control of the position or alignment of the transfer device
Abstract
A device for simultaneously dispensing a solution containing a sample to a test device and aspirating the sample from the device. In one embodiment the device is hand-held and includes a control mechanism, e.g., button for activating the dispensing and aspiration of the sample. The testing device may take the form of a multi-well plate with a plurality of wells arranged in rows and columns, with the bottom of the wells formed as a photonic crystal biosensor.. The device can be configured with dispense and aspirate manifolds and associated dispense and aspirate ports which are positioned in all the wells along a row or column of wells so as to simultaneously aspirate and dispense solution to all the wells in a row or column of the multi-well plate.
Claims (30)
1. A device for simultaneously dispensing and aspirating a sample to a testing device comprising:
a) a body having a portion thereof adapted for engagement with the testing device;
b) dispense tubing coupled to the body for receiving a sample from a source;
c) aspirate tubing coupled to the body connected to a source of vacuum;
d) a dispense manifold connected to the dispense tubing having at least one dispensing port;
e) an aspirate manifold connected to the aspirate tubing having at least one aspirating port; and f) a control mechanism for controlling movement of the sample from the dispense tubing to the dispense port and for simultaneously controlling application of vacuum in the aspirate tubing to the aspirating port.
a) a body having a portion thereof adapted for engagement with the testing device;
b) dispense tubing coupled to the body for receiving a sample from a source;
c) aspirate tubing coupled to the body connected to a source of vacuum;
d) a dispense manifold connected to the dispense tubing having at least one dispensing port;
e) an aspirate manifold connected to the aspirate tubing having at least one aspirating port; and f) a control mechanism for controlling movement of the sample from the dispense tubing to the dispense port and for simultaneously controlling application of vacuum in the aspirate tubing to the aspirating port.
2. The device of claim 1, wherein testing device comprises a multi-well testing device arranged in one or more rows of a plurality of wells, and wherein the dispense and aspirate manifolds include a dispensing and aspirating port for each well in the row of wells in the multi-well test device.
3. The device of claim 1, wherein the body is sized and shaped so as to be held in a human hand and wherein the control mechanism includes a manually-activated control device incorporated into the body,
4. The device of claim 3, wherein the control device activates a valve coupling the dispense tubing to the dispensing manifold and a valve coupling the aspirate tubing to the aspirate manifold.
5. The device of claim 1, wherein the device further comprises an auxiliary injection port for receiving a second sample for introduction to the testing device.
6. The device of claim 1, wherein the testing device includes a plurality of wells for receiving the sample and wherein the wells have a bottom surface for receiving the sample constructed as a grating-based biosensor.
7. The device of claim 6, wherein the body further comprises a lower surface for engaging a top surface of the testing device, and wherein the testing device further comprises a plurality of wells, and wherein the dispensing and aspirating ports further comprise tips thereof which extend into wells formed in the top surface of the testing device when the lower surface of the body is engaged with the top surface of the testing device.
8. The device of claim 1, wherein the control mechanism is manually operated.
9. The device of claim 1, wherein the control mechanism is automatically operated.
10. The device of claim 1, wherein the device comprises at least eight aspirating ports and at least eight dispensing ports.
11. The device of claim 10, wherein the aspirating and dispensing ports are spatially arranged in at least eight pairs of aspirating and dispensing ports.
12. The device of claim 1, further comprising a variable flow pump for directing sample to the testing device via the dispense tubing.
13. The device of claim 1, wherein the device further comprises at least one sensor for measurement of a sample delivered by the device to the testing device.
14. The device of claim 13, wherein the sensor selected from the group of sensors consisting of a) a temperature sensor, b) a pH sensor, and c) an ionic strength sensor.
15. The device of claim 13, wherein the at least one sensor is mounted adjacent to at least one of the aspirating port or the dispensing port.
16. The device of claim 13, further comprising a processing unit controlling delivery of the sample to the testing device using a feedback loop incorporating sensor data reported by the at least one sensor.
17. The device of claim 1, wherein the device further comprises a temperature controller controlling the temperature of the sample delivered to the testing device.
18. The device of claim 1, wherein the aspirate and dispense manifolds comprise elongate tubular channels having a length L, wherein the testing device comprises an array of wells arranged in rows and columns, and wherein at least one of the rows and columns of wells is of a linear dimension M, where L >= M, and wherein there is one aspirating port and one dispensing port in a spaced relation along the length of the aspirate and dispense manifolds for each well in the rows or columns of wells of length M.
19. A method of simultaneously dispensing and aspirating a sample to a testing device having at least one well, comprising the steps of:
positioning an aspirating and dispensing device over the testing device such that an aspirating port and a dispensing port in the device are placed into the at least one well;
activating a control mechanism to thereby cause a sample to enter the well via the dispensing port and simultaneously aspirating the sample from the well.
positioning an aspirating and dispensing device over the testing device such that an aspirating port and a dispensing port in the device are placed into the at least one well;
activating a control mechanism to thereby cause a sample to enter the well via the dispensing port and simultaneously aspirating the sample from the well.
20. The method of claim 19, wherein the testing device comprises a multi-well device having a plurality of wells arranged in rows and columns, and wherein during the positioning step an aspirating port and a dispensing port are placed into all the wells in a row or column of wells of the multi-well device.
21. The method of claim 19, wherein the activating step is performed manually.
22. The method of claim 19, wherein the method further comprises the step of sensing the sample with a sensor selected from the group of sensors consisting of a) a temperature sensor, b) a pH sensor, and c) an ionic strength sensor.
23. The method of claim 22, wherein the sensor comprises a temperature sensor and wherein the method further comprises the step of controlling the temperature of the sample delivered to the test device.
24. The method of claim 22, wherein the sensing step is performed with a sensor placed in the sample well in proximity to the dispensing port and the aspirating port.
25. The method of claim 19, further comprising the step of making an optical measurement of the testing device simultaneously with the activating step.
26. The method of claim 25, wherein the testing device comprises a grating-based biosensor, and wherein the optical measurement comprises a measurement of a shift in peak wavelength value of a sample dispensed onto the surface of the biosensor.
27. The method of claim 19, further comprising the step of introducing a second sample onto the testing device during the activating step.
28. The method of claim 27, wherein the second sample is introduced via an auxiliary injection port provided in the aspirating and dispensing device.
29. The method of claim 19, wherein the testing device comprises a photonic crystal biosensor, and wherein the method further comprises the step of making a measurement of a shift in peak wavelength value from a well of the biosensor during the simultaneous aspirating and dispensing of the sample.
30. A method of making a measurement of binding affinity of a sample, comprising the steps of:
introducing the sample into a well having a bottom formed as a photonic crystal biosensor, wherein a portion of the sample becomes bound to the biosensor;
making a measurement of the change in the shift in peak wavelength value as a function of time (k on) from the well as the sample is introduced into the well;
simultaneously aspirating and dispensing the sample from the well and measuring the change in the shift in peak wavelength value as a function of time (k off) during the simultaneous aspirating and dispensing, calculating an equilibrium association constant or an equilibrium dissociation constant for the sample from the values of k on and k off.
introducing the sample into a well having a bottom formed as a photonic crystal biosensor, wherein a portion of the sample becomes bound to the biosensor;
making a measurement of the change in the shift in peak wavelength value as a function of time (k on) from the well as the sample is introduced into the well;
simultaneously aspirating and dispensing the sample from the well and measuring the change in the shift in peak wavelength value as a function of time (k off) during the simultaneous aspirating and dispensing, calculating an equilibrium association constant or an equilibrium dissociation constant for the sample from the values of k on and k off.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11/601,020 | 2006-11-17 | ||
| US11/601,020 US7628085B2 (en) | 2006-11-17 | 2006-11-17 | Simultaneous aspirator and dispenser for multiwell plates and similar devices |
| PCT/US2007/019566 WO2008060347A1 (en) | 2006-11-17 | 2007-09-07 | Simultaneous aspirator and dispenser for multiwell plates and similar devices |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2637669A1 true CA2637669A1 (en) | 2008-05-22 |
| CA2637669C CA2637669C (en) | 2012-01-24 |
Family
ID=39401973
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2637669A Expired - Fee Related CA2637669C (en) | 2006-11-17 | 2007-09-07 | Simultaneous aspirator and dispenser for multiwell plates and similar devices |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US7628085B2 (en) |
| EP (1) | EP2082240A1 (en) |
| JP (1) | JP2009532704A (en) |
| AU (1) | AU2007302639B2 (en) |
| CA (1) | CA2637669C (en) |
| NZ (1) | NZ568184A (en) |
| WO (1) | WO2008060347A1 (en) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7167615B1 (en) | 1999-11-05 | 2007-01-23 | Board Of Regents, The University Of Texas System | Resonant waveguide-grating filters and sensors and methods for making and using same |
| US8111401B2 (en) | 1999-11-05 | 2012-02-07 | Robert Magnusson | Guided-mode resonance sensors employing angular, spectral, modal, and polarization diversity for high-precision sensing in compact formats |
| US7371562B2 (en) | 2000-10-30 | 2008-05-13 | Sru Biosystems, Inc. | Guided mode resonant filter biosensor using a linear grating surface structure |
| US7524625B2 (en) * | 2000-10-30 | 2009-04-28 | Sru Biosystems, Inc. | Real time binding analysis of antigens on a biosensor surface |
| US7118710B2 (en) | 2000-10-30 | 2006-10-10 | Sru Biosystems, Inc. | Label-free high-throughput optical technique for detecting biomolecular interactions |
| US7575939B2 (en) | 2000-10-30 | 2009-08-18 | Sru Biosystems, Inc. | Optical detection of label-free biomolecular interactions using microreplicated plastic sensor elements |
| US7927822B2 (en) | 2002-09-09 | 2011-04-19 | Sru Biosystems, Inc. | Methods for screening cells and antibodies |
| US7309614B1 (en) | 2002-12-04 | 2007-12-18 | Sru Biosystems, Inc. | Self-referencing biodetection method and patterned bioassays |
| US8298780B2 (en) | 2003-09-22 | 2012-10-30 | X-Body, Inc. | Methods of detection of changes in cells |
| US20060275825A1 (en) * | 2005-04-12 | 2006-12-07 | Sru Biosystems, Inc. | Proteolipid membrane and lipid membrane biosensor |
| US7628085B2 (en) * | 2006-11-17 | 2009-12-08 | Sru Biosystems, Inc. | Simultaneous aspirator and dispenser for multiwell plates and similar devices |
| US9134307B2 (en) | 2007-07-11 | 2015-09-15 | X-Body, Inc. | Method for determining ion channel modulating properties of a test reagent |
| AU2008274978A1 (en) | 2007-07-11 | 2009-01-15 | Sru Biosystems, Inc. | Methods of identifying modulators of ion channels |
| US20090181359A1 (en) * | 2007-10-25 | 2009-07-16 | Lou Sheng C | Method of performing ultra-sensitive immunoassays |
| US8222048B2 (en) * | 2007-11-05 | 2012-07-17 | Abbott Laboratories | Automated analyzer for clinical laboratory |
| US8257936B2 (en) | 2008-04-09 | 2012-09-04 | X-Body Inc. | High resolution label free analysis of cellular properties |
| US8916113B2 (en) * | 2009-02-27 | 2014-12-23 | Hitachi High-Technologies Corporation | Chemical analyzer |
| US20130330761A1 (en) | 2012-06-12 | 2013-12-12 | Celcuity, LLC | Whole cell assays and methods |
| WO2015089380A2 (en) | 2013-12-12 | 2015-06-18 | Celcuity Llc | Assays and methods for determining the responsiveness of an individual subject to a therapeutic agent |
| CN103884710B (en) * | 2014-02-08 | 2016-05-25 | 上海交通大学 | A kind of method of utilizing photonic crystal wellability to measure pH |
| JP7264484B2 (en) | 2017-03-20 | 2023-04-25 | セルキュイティー インコーポレイテッド | Method for measuring signal transduction pathway activity for therapeutic agent selection |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3780912A (en) * | 1968-04-02 | 1973-12-25 | Micromedic Systems Inc | Metering and dispensing apparatus |
| JPH02135865A (en) * | 1988-11-16 | 1990-05-24 | Ricoh Co Ltd | Facsimile equipment |
| JPH0783939A (en) * | 1993-09-13 | 1995-03-31 | Aloka Co Ltd | Method and apparatus for suction for microplate |
| US5578270A (en) | 1995-03-24 | 1996-11-26 | Becton Dickinson And Company | System for nucleic acid based diagnostic assay |
| ATE250978T1 (en) * | 1997-11-14 | 2003-10-15 | Gen Probe Inc | WORKING DEVICE FOR ANALYSIS |
| CA2547296C (en) | 1997-12-04 | 2010-08-24 | Roche Diagnostics Corporation | Apparatus for determining concentration of medical component |
| US6537505B1 (en) | 1998-02-20 | 2003-03-25 | Bio Dot, Inc. | Reagent dispensing valve |
| US6096271A (en) | 1998-02-27 | 2000-08-01 | Cytologix Corporation | Random access slide stainer with liquid waste segregation |
| JPH11271330A (en) * | 1998-03-26 | 1999-10-08 | Tosoh Corp | Method for avoiding cross contamination between sample |
| WO2000044497A1 (en) | 1999-01-29 | 2000-08-03 | Genomic Instrumentation Services, Inc. | Pipetter |
| US6143252A (en) * | 1999-04-12 | 2000-11-07 | The Perkin-Elmer Corporation | Pipetting device with pipette tip for solid phase reactions |
| US6325114B1 (en) | 2000-02-01 | 2001-12-04 | Incyte Genomics, Inc. | Pipetting station apparatus |
| US6951715B2 (en) | 2000-10-30 | 2005-10-04 | Sru Biosystems, Inc. | Optical detection of label-free biomolecular interactions using microreplicated plastic sensor elements |
| US7142296B2 (en) | 2000-10-30 | 2006-11-28 | Sru Biosystems, Inc. | Method and apparatus for detecting biomolecular interactions |
| US7217574B2 (en) | 2000-10-30 | 2007-05-15 | Sru Biosystems, Inc. | Method and apparatus for biosensor spectral shift detection |
| US7118710B2 (en) | 2000-10-30 | 2006-10-10 | Sru Biosystems, Inc. | Label-free high-throughput optical technique for detecting biomolecular interactions |
| US7023544B2 (en) | 2000-10-30 | 2006-04-04 | Sru Biosystems, Inc. | Method and instrument for detecting biomolecular interactions |
| US20030162308A1 (en) | 2001-12-04 | 2003-08-28 | Dave Smith | Orthogonal read assembly |
| DE60317305T2 (en) | 2002-01-25 | 2008-08-28 | Innovadyne Technologies, Inc., Santa Rosa | CONTACTLESS METHOD FOR DISTRIBUTING LOW LIQUID QUANTITIES |
| US7068885B2 (en) * | 2004-03-24 | 2006-06-27 | Enablence, Inc. | Double diffraction grating planar lightwave circuit |
| US6990259B2 (en) | 2004-03-29 | 2006-01-24 | Sru Biosystems, Inc. | Photonic crystal defect cavity biosensor |
| WO2005124366A1 (en) * | 2004-06-14 | 2005-12-29 | Parker-Hannifin Corporation | Robotic handling system and method with independently operable detachable tools |
| PL369834A1 (en) * | 2004-09-07 | 2006-03-20 | PZ HTL Spółka Akcyjna | Electronic pipette apparatus for transferring and measuring defined quantity of liquid |
| JP2006242858A (en) * | 2005-03-04 | 2006-09-14 | Fuji Photo Film Co Ltd | Dispenser and measuring instrument utilizing attenuated total reflection |
| JP2006284350A (en) * | 2005-03-31 | 2006-10-19 | Fuji Photo Film Co Ltd | Dispenser, attaching method of pipette chip of dispenser and measuring instrument utilizing attenuation of total reflection |
| US7628085B2 (en) * | 2006-11-17 | 2009-12-08 | Sru Biosystems, Inc. | Simultaneous aspirator and dispenser for multiwell plates and similar devices |
-
2006
- 2006-11-17 US US11/601,020 patent/US7628085B2/en not_active Expired - Fee Related
-
2007
- 2007-09-07 NZ NZ568184A patent/NZ568184A/en not_active IP Right Cessation
- 2007-09-07 EP EP07837901A patent/EP2082240A1/en not_active Withdrawn
- 2007-09-07 JP JP2009504361A patent/JP2009532704A/en active Pending
- 2007-09-07 AU AU2007302639A patent/AU2007302639B2/en not_active Ceased
- 2007-09-07 WO PCT/US2007/019566 patent/WO2008060347A1/en active Application Filing
- 2007-09-07 CA CA2637669A patent/CA2637669C/en not_active Expired - Fee Related
-
2009
- 2009-07-15 US US12/460,288 patent/US7832291B2/en not_active Expired - Fee Related
- 2009-10-23 US US12/589,446 patent/US8061220B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| AU2007302639A1 (en) | 2008-05-22 |
| EP2082240A1 (en) | 2009-07-29 |
| NZ568184A (en) | 2011-05-27 |
| JP2009532704A (en) | 2009-09-10 |
| US20100043571A1 (en) | 2010-02-25 |
| US20090282931A1 (en) | 2009-11-19 |
| US20080115567A1 (en) | 2008-05-22 |
| WO2008060347A1 (en) | 2008-05-22 |
| CA2637669C (en) | 2012-01-24 |
| US8061220B2 (en) | 2011-11-22 |
| US7628085B2 (en) | 2009-12-08 |
| AU2007302639B2 (en) | 2010-01-14 |
| US7832291B2 (en) | 2010-11-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2637669A1 (en) | Simultaneous aspirator and dispenser for multiwell plates and similar devices | |
| US8372356B2 (en) | Manually directed, multi-channel electronic pipetting system | |
| US6983636B2 (en) | Apparatus and method for assessing the liquid flow performances through a small dispensing orifice | |
| US6039211A (en) | Position triggered dispenser and methods | |
| JP2006516738A (en) | Sample handling equipment | |
| US20080050287A1 (en) | Chemical reaction apparatus | |
| CA2392943A1 (en) | Chemistry system for a clinical analyzer | |
| EP3552705A1 (en) | Droplet dispensing apparatus | |
| US7369241B2 (en) | Continuous optical measuring apparatus and continuous optical measuring method | |
| US20010036425A1 (en) | Device for transferring samples of micro-amounts of liquids | |
| US20100119415A1 (en) | Dispensing device and automatic analyzer | |
| US20040241872A1 (en) | Optical detection liquid handling robot system | |
| US8453524B2 (en) | Solution feeding device | |
| US10682874B2 (en) | Droplet dispensing apparatus | |
| TWI733969B (en) | Pressure and leak testing methods | |
| US20100304498A1 (en) | Microchip reaction detection system and method of reaction of microchip in flow path | |
| JP2009103641A (en) | Inspection apparatus | |
| JP2001324510A (en) | Variable discharge capacity liquid dispensation device | |
| KR20180085216A (en) | Experimental Apparatus For The Cellular Metabolism Measurement |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20130909 |