CA2699485A1

CA2699485A1 - Tissue marking compositions

Info

Publication number: CA2699485A1
Application number: CA2699485A
Authority: CA
Inventors: Satish Agrawal; Roger Boggs
Original assignee: Performance Indicator L.L.C.; Satish Agrawal; Roger Boggs
Current assignee: Performance Indicator LLC
Priority date: 2007-09-13
Filing date: 2008-09-11
Publication date: 2009-03-19
Also published as: AU2008299985A1; EP2187812A1; EP2187812A4; JP2010540416A; US20090071365A1; US7842128B2; WO2009035622A1

Abstract

Provided are permanent tissue markings comprising at least one colored compound comprising a thermally activatable fragmentation group and at least one infrared absorbing compound, wherein the at least one colored compound is capable of being rendered colorless by unimolecular fragmentation of the thermally activatable fragmentation group when the tissue marking is non-imagewise exposed by a source of infrared radiation.

Description

TISSUE MARKING COMPOSITIONS
Backeround of Invention This invention relates generally to tissue markings that are normally permanent, but can be erased that is, rendered colorless when desired. In particular, this invention relates to tissue marking, which can become colorless when infrared radiation is applied as well as to methods of their use.
Tattoos, which are a form of tissue markings, have been in use for thousands of years by many cultures for many purposes including artistry, beauty, identification, and religious purposes. Today the majority of tattoos are used for artistic expression as well as cosmetic applications such as permanent lip coloration, eyebrow coloration and eyeliner. Other uses for tattoos include corrective pigmentation following surgery and identification markings on animals.
The tattooing procedure consists of piercing the skin with needles or similar instruments to introduce an ink that typically includes particles of pigment suspended in a liquid carrier. Pigment particles that do not enter the dermis (larger pigment particles) and remain in.the epidermis are sloughed off over time, whereas enough of the particles (smaller particles) that get lodged in the dermis are phagocytosed by dermal cells or retained in the extracellular matrix to create permanent markings. It should be noted that some of the ink particles in the dermis, particularly the minute particles may potentially be removed and/or relocated by the body's biological processes. Thus, a permanent tattoo is created when a sufficient number of pigment particles introduced into the body are retained in the dermis. Typical tattoo pigments include carbon black, inorganic metal salts and colored organometallic complexes.
Tattoos or tissue marking ingredients have not yet been regulated or fully disclosed to the public and have been known to cause allergic reactions which in some cases can be severe even well after the time of tattooing, or after exposure to sunlight or-laser treatments. Despite the fact that there is a paucity of data in the lite-rature concerning the toxicity and carcinogenicity of tissue markings as well as their long their long term effects on the body, they continue to be used today.

Statistics have shown that a large portion of people who have tattoos wish to'have them removed. Since tattoos are generally designed to be permanent, their removal is very. difficult. Overtattooing, dermabrasion, and. surgical excision are typical "removal"
methods as well as the more current method of using pulsed lasefs: In laser removal;..
intense pulses of laser.energy are specifically absorbed by the tissue marking particles:
However, such methods require lasers emitting visible radiation; which consequently;
depending upon the tattoo particle color and hence the- wavelength of the laser radiation used, will result in absorption of the radiation by the skin and surrounding tissue, and thus cause collateral damage.
Such methods of tattoo removal can- be categorized as "tattoo removal by dispersal" since the laser causes the pigment particles to break into 'minute particles for dispersal by the body's biological processes such as lymphatic transport systeui and/or immune processes. Using laser techniques to remove tattoos that utilize current tissue marking compositions have a number of disadvantages. Because of the reliance on ' "tattoo removal by dispersion", the body is .not only exposed to additional health haiards but also multiple treatments are required which are not only expensive but canbe painful.
Additionally, multiple lasers are needed for multicolored tattoos, while some pigments such as green and. yellow are virtually impossible to reniove.:
U.S. Patent No. 6,013,122 discloses removable tissue markings wherein pigment or dye particles are immobilized by avehicle which surttounds such dye or pigment;. that is, the colored particles are encapsulated in said vehicle and impiainted into the skin: The ".
vehicle, encasing the dye or pigment, ruptures when exposed to specific forms of energy, such as UV light or infrared energy and the pigment or dye is dispersed or dissipated ("tattoo removal by dispersal") from the location in which it was adniinistered; therefore erasing the tattoo image. It should be noted that tattoo removal by dispersal necessitates the colored particles to be small so that they can be dispersed to erase the tissue marking, which in turn requires the colored particles to be encapsulated, for otherwise; the tissue marking would not be permanent. As stated above "tattoo'removal by dispersal"
of the colored particles is inherently risky, as the body could be subjected to allergic or toxic reactions. If the colored compound is chosen to be biocompatible, then the choice of suitable dues will be significantly narrowed. Thus, the requirement of biocompatibility will significantly narrow the choice of suitable dyes. It should'be noted that the-above invention does not envision tattoo-removal by render'iitg the dye or pigment colorless: _ U.S. Patent No. 6,814,760 provides for microparticles that create-permanent tissue=
markings, such as tattoos, designed in advance for change and/or removal on deinand, as well as methods for, implanting the microparticles in tissue and methods to change and/or remove the implanted markings. The micropaiticles contain chromophores (colored particles).which are encapsulated by coating materials by a variety~of encapsulation techniques such as aerosol collision, chamber deposition etc.
Two embodiments for the removable tissue marking compositions, which are in the form of microparticles, are envisioned. In the first embodiment, microparticles which-contain chromophores (colored compound particles) are constructed such that tattoo removal is accomplished by dispersal of the colored compound particles, that is, the dispersal is achieved by making the microparticles permeable; such as by rupture of the coating comprising the microparticle. The chromophore (colored particles) is dispersed either by dissolution.in bodily fluids, or by biological processes such as meatabolism, lymphatic transport etc. It should be noted that tattoo removal by dispersal necessitates the colored particles to be small so they can be dispersed to erase the tissue marking which in turn requires the colored particles to be encapsulated; for otherwise, the tissue marking would not be permanent. Relying on dispersion as the removal-technique exposes the subjects to health hazards by virtue of some of the tattoo particles entering the body such as in the lymphatic system.
In the second embodiment, microparticles can contain chromophores that'are rendered invisible in-situ, that is, without rupturing the microparticles, and'the chromophore does not need to be dissolved, metabolized or dispersed for removal of the marking. Thus the tissue markings can become invisible without the need for being released into the bodily fluids: This embodiment generally requires that the coloied compound be selected such that it canabsorb the triggering"electromagnetic radiation.
Lasers used to deliver the energy for tattoo removal for these bleachable chromophores would either be near UV, visible, or near infra red.
For the case wherein the tissue marking is rendered colorless withnear UV -energy, apart from health hazards to the subjects being exposed to such UV
energy, the -chromophores of such tissue markings will generally be subject- to photolytic degradation, and hence, such tissue markings will be vulnerable to fading by prolonged exposure to sunlight, adversely affecting their permanence until the 'desire for removal.
Use of tissue markings that can be rendered colorless or bleachable: by visible lasers will be similar to the cunent practice of tattoo removal, requiring multiple visits and, additionally, will not be devoid of tissue scarring or injury. Intense visible light'can target the skin's natural pigment, melanin, resulting in temporaryor peimanent -hypopigmentation or hyperpigmentation, especially in-dark or tanned skin;
and/or hair loss in the area. -The near infra red bleachable tissue markings will-generally-have colors in the red portion of the visible spectrum thereby severely limiting the color choices for the tissue markings.
Embodiments wherein the tissue marking.is rendered colorless without rupturing the microcapsule, and not requiring the chromophore of the tissue marking-to absorb the triggering radiation,.that is, requiring the colored compound to contain a specific radiation absorbing component, is one wherein heat is used to -release a second reactive component such as a strong acid, or strong oxidizing agent, or a thermal initiator generating free radicals which then must first come into contact with the pigment' or dye and then react .with it to bleach the chromophore. One can question the practice of introducing strong oxidizing/reducing agents or free radicals in the human body: Even though such materials may be encapsulated, they would pose significant health hazards since there is always a danger of leakage from the capsule. Further, such -embodiments -can result in fairly complex microcapsule constructions. . : -Thus there is a need for new, on-demand removable tissue- marking compositions and methods for their use that are simple, that do not require use of hazardous materials for accomplishing the color removal, that do not requiredispersal of the tissue marking for removing the marking, that is, markings wherein the color can be switched off in -situ, that embody methods of color change which do not adversely impact tattob permanence, that minimize or eliminate the damage to the skin and surrounding-tissue, and wherein the tattoo removal method would be applicable to a wide variety of colored, -materials.

Summary of Invention The present invention provides for permanent tissue-marking compositions that can be used as tissue markings whi,ch have been predesigned to be removable on demand;
wherein, the tissue marking compositions do not require anyother reactive species to effect color removal, that is the color change is a unimolecular reaction, and wherein markings can be removed in a single treatment by exposure to infrared radiation. -There are many advantages of the inventive ink compositions of this invention.
One-advantage is that these compositions do not necessarily require any specialized vehicles such as encapsulated colored particles for use as permanent tissue markings.
That is; tht colored compositions of this invention, which comprise at least one colored particle and an infrared absorber, are not required to undergo encapsulation for use as a permanent tissue marking. A second advantage is that since color removal does not require any additional reactive species, there are no additional health hazards to deal with. A third advantage of the inventive ink compositions. is that the colored particles do not have to be dispersed or removed from the tissue for color removal'as the color is switched off due to non-imagewise exposure to infrared radiation:. It should be noted that the now colorless, formerly colored, composition continues to exist as before in its immobilized state. A-fourth advantage of the inventive ink compositions is their ability to be of any color and - 20 become colorless using the same infrared energy. A fifth advantage of the inventive ink compositions is their ability to become colorless using infrared radiation, such that the surrounding skin is substantially transparent to the infrared radiation. The present invention also includes methods of applying the inventive ink compositions and-methods of rendering the ink colorless. .
In a first aspect, the present invention provides for a colored composition for use as a tissue marking which includes at least one colored compouiid containing a thermally activated fragmentation group and at least one infrared absorbing compound in which the at least one colored compound is capable of being rendered colorless by unimolecular .
cleavage of the fragmentation group upon exposure of the tissue marking to a source of infrared radiation.

In a second aspect, the present invention provides for a method of applying a colored composition to a tissue by implanting into: the. tissue an amount of the tissue marking in sufficient quantity such that enough of the colored composition is-retained in the tissue to form a permanent detectable marking until desired to be erased, said ink containing at least one colored compound containing a thermally activated fragmentation group,-and at least one infrared absorbing compound wherein the at least ocie colored -compound is capable of being rendered colorless by unimolecular cleavage of the fragmentation group upon exposure of the tissue marking to a- source of infrared radiation.
In a third aspect, the present invention provides for a method of removing the tissue marking by implanting into a tissue an amount of the colored ink composition in sufficient quantity to form a detectable marking, said ink containing at least one colored compound comprising a thertnally activated fragmentation-group and at. least one infrared absorbing compound wherein the at least one colored compound is capable of being rendered colorless by unimolecular cleavage of the fraginentation group upon exposure of the tissue marking to a source of infrared radiation and applying infrared-radiation to a sufficient amount of the tissue marking to render the ink colorless:

Brief Description of the Drawines Figure 1 provides a general representative structure of a functionalized colored compound useful for the current invention.
Figure 2A depicts the structure of a magenta colored compound suitable for the present invention.
Figure 2B depicts the structure of the colorless compound that results when the t-butyl-carbonyl group of the compound of Figure 2A, having received sufficient thermal energy, fragments and the resultant nucleophilic nitrogen cyclizes with the chromophore.
Figure 3 represents a structure employing an oxime fragmentation.group useful in lowering activation temperature. - -Figure 4 represents a structure employing an oxime fragmentation group that exhibits an activation temperature of 120 C. -Figure 5 represents a structure employing. a cycloalkyl trigger'group useful in achieving higher activation temperatures.
Figure 6 depicts the structure of a functionalized colored compound.coiitaining ballast groups useful for the current invention.
Figure 7 depicts another, colored compound suitabl'e for the present invention, wherein a xanthene chromophore has attached to it a CH2- spacer group; which is attached to a sulfur nucleophile and attached to the nucleophile is a thermally activatable fragmentation group containing a ballast group.

Detailed Description ofthe Invention The present invention provides for permanent tissue niarking compositions, which are pre-designed to be permanent until the desire for removal, that is, removable on demand, and transition to a colorless state by application of non-imagewise infrared radiation.
As used herein the term "colored compound" refers to dyes and- pignierits that are visible to the unaided eye under normal lighting conditions such as in diffuse sunlight or standard artificial lighting.
The term "dye", as used herein, refers to a material, which contains a chromophore that allows the material to be visible to the unaided eye under normal lighting conditions.
As used herein, the term "colorless" refers to that state of a colored compound wherein, relative to the original color, the color is reduced by-a minimum of 60% in the colorless state.
A"chromophore", as used herein, refers to the part of a molecule responsible for ' its color.
As used herein an "indispersible substance" refers to particulate matter used for tissue markings that is on average large enough such that a sufficient number of the particles are retained when injected into the body to form a permanent tissue marking, that is, a sufficient number of the particles do not disintegrate, dissolve, become metabolized in tissue or relocated/eliminated by the body's biological processes. It should be noted that some number of the individual microparticles may be relocated from the tissue marking site through biological processes (such as lymphatic transpoct).
As used herein a "colored composition" refers to-an admixture of at least one indispersible colored compound and one indispersible infrared absorber-to render a tissue marking As used herein a "tissue marking" comprises a colored composition which after implantation. into the tjssue results in a permanent marking in the-tissue until such time it is desired to be removed. Tissues are inclusive of including skin, iris, sclera, dentin, fingernails, toenails, tissue beneath fingernails, tissue beneath toenails, tissue inside the mouth, and tissue lining internal body passages.
10. As used herein, the term "thermally activated" refers to the property of a material.
that undergoes change when exposed to a particular temperature.
The term "trigger", as used herein, refers to:the therrrially activated portion of a -fragmentation group. The trigger may be a partor the entu'e tliernially activated fragmentation group.
As used herein, "fragmentation" refers to the cleavage of a'molecule by the scission of one or more chemical bonds.
The tenm "unimolecular fragmentation", as used herein, refers to the cleavage of a single molecule by scission of one or more chemical bonds, caused without the interaction or involvement of a secondary molecule.
The term "nucleophilic group", as used herein, refers to a group containing a free pair of electrons that are capable of reacting.
The term "internal cyclization" refers to a reaction in which a substituent on a molecule undergoes a reaction with the said molecule resulting in a cyclic product.
The term "ballast group" refers to a group attached to a molecule to tailor into the molecule desired characteristics such as to retard the mobility of a molecule or portions thereof, such mobility emanating from either chemical or physical interactions or both or to impart biocompatibility or both, or to aid in the ability to formulate the-composition or the like.
As used herein, the term "binder" refers to a material; generally a polymeric species, that is used to make various components in a composition reside together in proximity As used herein, a"biologically inert" material refers to 'materials which have no significant biological effect when implanted into the body.
As used herein, a "biocompatible material" refers to materials which have no significant adverse biological effect when implanted into the body.
As used herein "non-imagewise exposure" refers to an exposure, which does not.
differentiate between imaged and non imaged areas of a marking or image: In imagewise exposure, some areas are exposed while other areas are_ not to cause a differentiation between an exposed.and an unexposed area, hence creating an image. Non-imagewise exposure is an exposure protocol that during the exposure step does not differentiate between the areas that have markings, or images, and areas that do not. As such non=
imagewise exposure may be a broad, blanket exposure or a scanning exposure. In the current invention the non-imagewise exposure is designed to eliminate any image of the tissue marking, such that no image remains.
The present invention provides for tissue markings,. and methods of theic use, which are suitable as permanent tissue markings, and which are capable of having-their color rendered colorless upon application of infrared radiation. The tissue markings -useful in the present invention include at least one colored compound that contains or has been modified to contain a nucleophilic group as well as a thermally activatable fragmentation group, which upon.fragmentation will render the colored compound .20 . colorless.
The tissue markings of this invention can be rendered colorless by a-unimolecular fragmentation, that is, they do not require reaction with another compound to achieve the transition to a colorless state. It should be noted that the use of above stated concept of fragmentation to render the tissue marking colorless can result in low residual densities or d-min without requiring any strong secondary oxidizing or reducing agents.
Hence, the concept of thermally triggered, unimolecular fragmentation to create removable, on demand, tissue marking is of great benefit since,btherwise, the use of a second component such as a strong oxidizing or reducing agent to render the tissue marking colorless would pose a hazard to the body. Additionally the colorless species continues to exist in its immobilized state.

It should be further noted that the transition of the tissue marking-to a colorless -state can be accomplished by a non imagewise exposure to infrared radiation, because the areas of the body which do not have any tattoo would also not-have any infrared absorber and hence will be essentially unaffected by the exposure.
The tissue markings of this invention also include those wherein a suitable.
ballast group is attached to the selected colored compound. The ballast groups- can be selected to -enhance specific characteristics of the colored compound. For example; a ballast group can be selected to further restrict the mobility of the tissue marking, or to enhance the colored compound biocompatibility, or to aid in forniulating the tissue marking.
By incorporating an infrared compound whose absorption wavelength is selected such that the skin is essentially transparent to the radiation, one can minimize or eliminate damage to the skin and tissue. Hence, unlike today's practice, the tissue markings of this invention can be designedto be rendered colorless with minimal to no damage of, the skin or tissue. It should also be noted that the tissue markings of this invention are rendered colorless without the dispersal of the colored compound into the human body. It is believed that when infrared radiation from an infrared radiation source, _ such as a laser, is applied to the tissue marking composition, the infrared absorbing compound absorbs the radiation and transfers heat to the colored, compound with the attached fragmentation group in an amount sufficient to cause the cleavage of the ' fragmentation group with subsequent internal cyclization to render the compound colorless.
It would be advantageous to ensure proximity of the colored compound and the infrared absorbing compound within the tissue marking. Close proximity will-ensure efficient heat transmission to the colored compound thereby minimizing the infrared energy required for transition to the colorless state. It should be noted that one can select the fragmentation group such that on fragmentation either the fragmented species can become gaseous and escape from the body or the fragmented species remains behind in the body. Depending on the specific fragmentation group selected, in some cases, such as the case wherein the fragmented group is left behind, it may be necessary to have a.
ballast group attached to the fragmentation group to ensure its immobility upon fragmentation or cleavage from the colored compound.

In general one can create a functionalized colored compound that-is controlled by a trigger group, commonly referred to as a protective group, which can be-removed by changes in pH, electromagnetic radiation, thermally; or under a number of other conditions. Removal of the trigger group creates a molecular.cascade in which a nucleophilic atom, such as oxygen, nitrogen, sulfur, carbon, silicon, phosphorus;
selenium, or their oxides, are eliminated as a result of the- loss-of the trigger group -and the nucleophile attacks and interrupts the chromophore rendering it essentially colorless:
Examples of colored materials containing triggergroups that canbe removed by alkali wherein the nucleophilic groups are oxygen- or nitrogen-based can be found in U.S. Patent No. 4,304,833.. We have found that those fragmentation groups capable of undergoing alpha-beta elimination (cited in U.S. Patent No. 4,304,833) associated with nucleophiles that are based on oxygen or nitrogen can also be triggered by heat and hence infrared radiation. Examples of carbamate fragmentation groups associated"with nitrogen based nucleophiles that are thermally triggered for use with imagewise exposure can be found in U.S. Patent No. 4,602,263..
A general representative structure of a functionalized colored compound useful -for the current invention is shown in Figure 1. In Figure1, D is a chromophore of the -colored compound, Sp is a substituted or unsubstituted optional spacer group connecting the colored compound to a nucleophile. Nu, Nu is a nucleophile which can be substituted or unsubstituted, and FG is a thermally activated fragmentation group, which could be the trigger group in its entirety or may contain the trigger group as well as other species.
Upon cleavage of the fragmentation group, the nucleophile enables a cyclic structure of D-Sp-Nu, which is essentially colorless.
Suitable colored compounds, represented by D in Figure 1, include, for example, -substituted and unsubstituted triarylmethane dyes,xanthene dyes, rhodamines dyes;
fluoran dyes, azocarbocyanine dyes, thiazine dyes, acridine dyes and aminoanthroquinone dyes, or other dyes.

Suitable optional spacer group connecting to the colored compound, represented by Sp in Figure 1, include, for example, substituted.and unsubstituted alkyl groups such as methylene, ethylene, and propylene groups, thionyl groups, cycloaliphatic groups and the like. The Sp group is selected to facilitate the formation of a favorable ring size upon internal cyclization to render the colored compound colorless.
Suitable nucleophiles, represented by-Nu in Figure l, include, for example, substituted or unsubstituted nucleophilic group with a free pair of electrons capable of cyclization, for example, substituted or unsubstituted nitrogen, oxygen, sulfur, phosphorus, carbon, selenium, and silicon.
Suitable thermally activatable fragmentation groups, represented by FG in Figure 1, include, for example, substituted and unsubstituted carbonaies, carbamates, esters, lactams, lactones, amides, imides, oximes, sulfonates, sulfinates, sulfenates, phosphates, and phosphonates and ---R2C-RHC-Y, wherein R is the same or different and comprises substituted or unsubstituted alkyl, aryl, or aralkyl groups and wherein Y is an electron withdrawing group, for example, cyano, nitro, a sulfoxide; a carbonyl containing compound, a sulfonamide or, for example, an electron withdrawing group having a positive sigma value greater than 0.6 as defined by Hammett's Equation,and other electron-withdrawing substituents that are capable of fragmenting when a sufficient amount of thermal energy is applied.
Figure 2A depicts a magenta-colored compound suitable for the present invention.
In Figure 2A, a xanthene chromophore has attached to it an SO2 spacer group, which is -attached to a nucleophilic methylamine group and attached to it is a thermally activatable fragmentation t-butoxy-carbonyl group with the t-butyl being a-trigger group.
When the compound of Figure 2A receives sufficient thernmal energy, the t-butyl-carbonyl group fragments and the resultant nucleophilic nitrogen cyclizes with.the chromophore to give the colorless compound in Figure 2B.
We have also found that the thermally activatable fragmentation groups can.
contain trigger moieties that can be selected to adjust the kinetics of the colored to colorless transition. For example, the t- butoxy-carbonyl. thermally activatable fragmentation group containing the t-butyl trigger group (-COO-t-But in Figure 2) can be thermally activated to trigger at temperatures of approximately 160 C=180 C.
when tested "
by heating on a hot plate. A lower activation temperature, which would result in faster fragmentation kinetics, can be achieved by use of an oxime fragmentation group. (See Figure 3).

Colored compounds containing an oxime thermally activated fragmentation group of the type shown in Figure 4 exhibits an activation temperature of 120 -C.- A
higher activation temperature (hence, slower kinetics) can be achieved by use of a thermally activatable fragmentation group containing a cycloalkyl trigger group (See:
Figure 5). One can adjust the kinetics of the fragmentation and hence the kinetics of the colored to colorless transition by adjusting the degree of electron withdrawing capability in the fragmentation group.
Having the ability to adjust the activation temperature that is, the temperature at which the fragmentation group cleaves at an acceptable rate, is an important design criterion for the tissue markings. It can be appreciated that to ensure permanence, the kinetics of fragmentation should be extremely low at body temperatures: This critsrion would drive selection of the activation temperature towards higher values.
However, too high an activation temperature will result in requiring higher amounts of infrared energy whiclr in turn will increase vulnerability of tissue to heat damage. Hence the goal will be to select an activation temperature high enough to ensure tissue marking permanence but not require excessive amounts of energy.
The tissue marking compositions of the cun:ent invention further-include infrared absorbing compounds which remain present in the permanent tissue marking. When it'is desirable to "remove" the tattoo, that is, render the tattoo colorless; the infrared absorbing compound absorbs infrared radiation and. the heat generated from the absorption ti=ansfers to the thermally substituent of the colored compound which then fragments:
Infrared absorbing compounds that are useful for the current invention include, for example;
cyanine, squarylium, azulenium, indophenol, naphthoquinone, and anthraquinone - compounds. Typically these.compounds have little to no absorbance in the visible region of the electromagnetic spectrum,.thus causing no interference with the color of the tissue marking or when the tissue marking is rendered colorless. Examples of infrared absorbing compounds are disclosed in U.S. Patent No. 5,409,797 herein incorporated by *
reference for all purposes.

As stated above, the colored compound or infrared absorbing compound may 30 optionally have attached to it one or more ballast groups... In the case of the.colored compound a ballast group may be attached to the chromophore portion, the fragmentation group, or both, or on other portions of the molecule. Ballast groups are designed to add predetermined properties to the ink where they are needed. For example, since it is, important for tissue markings to be biocompatible in the dermis so as to have na significant biochemical, allergic or immune response bythe body after the normal healing period, ballast groups may be attached to the components of the tissue marking to aid in biocompatibility. Ballast groups attacheii to infrared absorbirig compound, by restricting their mobility, can assist in ensuring proximity of the colored compound and the infrared absorbing compound for high efficiency in transferring the heat to the colored compound.
One can also achieve close proximity of the colored coinpound and the infrared absorber by attaching affinity groups (for example to create'hydrogen bonding) to the colored compound, or the infrared absorber or both. Alternatively, the colored compound and the infrared absorber can be encapsulated. It can be appreciated that closest proximity will be achieved by covalently or ionically linking the infrared absorber aiid the colored compound.
As stated above, the ballast group can be attached to different components of the colored compound as needed to tailor desired characteristics into the compound. For example, a ballast group may be attached to the fragmenting group to enhance its immobility after fragmentation. Another example is the ballast group could be attached to the chromophoric portion of the colored compound to further enhance its immobility in the tissue. A further example is a ballast group may be attached to any portion of the colored compound to aid in the formulation of the composition. A
represeptative structure of a functionalized colored compound- containing ballast groups*
useful for the cunent invention is shown in Figure 6.
_ One or more positions on the colored compound can be substituted with one or more types of ballast groups to exhibit the desired property. The ballast groups. are chosen so as not to interfere with the "removal" process, for example, by being essentially transparent to the infrared radiatioii: In Figure 5 there are three ballast groups shown to illustrate useful positions, two ballast groups are attached to the chromophore of the colored compound and one ballast group is attached to the fragmentation group. Also in this example the spacer group, Sp, is methylene, the nucleophile; Nu, is oxygen and the fragmentation group, FG, is ---CH2CH2-S02-=-Ballast.
Figure 7 depicts an additional colored compound suitable for the present invention. In Figure 7, a xanthene chromophore has attached to it a CH2 spacer group, which is attached to a sulfur nucleophile and, attached to the nucleophile, is a thermally activatable fragmentation group containing a ballast group.
It should be noted in Figures 6 and 7 that the ballast group that is attached to the FG fragmentation group would also allow the fragment to have certain predetermined properties such as, for example, improved biocompatibility or improved immobility, as well as, if desired, aiding in formulating the tissue marking composition.
Ballast groups useful in the present invention include long chain fatty acids and fatty alcohols, gums, natural waxes, glycols, polyglycols, glycerol esters, gelatins, lipids, phospholipids, arabinogalactan, glutaraldehyde, petroleum wax, andmixtures thereof, poly(acrylic acid co-hypophosphorite) sodium salt, polyacrylamides, alginates, caseinates, polypectates, cellulosic materials, chitosan, glycerides, pectins, long chain polyacrylates, polymaleic acid and/or its sodium salts, polyvinyl acetate, sugars, polysorbate 80, polyvinylpolypyrrolidone, polyvinylpyrrolidone, and poly(20 vinylpyridine=co-styrene):
poly(hexadecyl acrylamide), poly(butyl acrylate), poly(hexadecyl- acrylate), poly(octadecyl acrylate), poly(dodecene), poly(isobutene), poly(trimethyl glutarate);
polyanhydides, polyorthoesters, polystyrene, polyurethane, polypropylene, polymethacrylate, polytetrafluoroethylene, and other known polymers which are compatible with biological tissue. The ballast group can be chosen to be of a size that aids in immobilization, such as, for example, between 5 and 300 Angstroms as well as chosen to be of a type that can interface with the surrounding tissue to aid in immobilization, for example, by hydrogen bonding.
The colored compositions that result in tissue markings of the current invention includes at least one colored compound and at least one infrared'absorbing compoiund and may be present in admixture with a liquid carrier, such as for example;
alcohol, water or-glycerin or other liquid carriers well know in the art or mixtures thereof, and.may additionally be admixed with binders, or encapsulated to give the desired tissiue marking., or as combinations thereof. The tissue marking, either in its colored or colorless form; is designed to remaiin indefinitely in the tissue.
As an admix with binders, the tissue marking of the current invention may include one or more binder materials which are present to help keep the dye aiid infrared -absorbing compound in close proximity to each other such that the heat generated by the infrared absorption may transfer more efficiently to the thermally labile group: Typical binder materials include, for example, long chain fatty acids and fatty alcohols, gums, natural waxes, glycols, polyglycols, glycerol esters, gelatins, lipids;
phospholipids;
arabinogalactan, glutaraldehyde, petroleum wax, and mixtures thereof, poly(acrylic acid co-hypophosphorite) sodium salt, polyacrylamides, alginates, caseinates, polypectates, cellulosic materials, chitosan, glycerides, pectins, long chain polyacrylates, , polymaleic acid and/or its sodium salts, polyvinyl acetate, sugars, polysorbate 80, polyvinylpolypyrrolidone, polyvinylpyrrolidone, and poly(20 vinylpyridine-co-styrene).
poly(hexadecyl acrylamide), poly(butyl acrylate), poly(hexadecyl acrylate), poly(octadecyl acrylate), poly(dodecene), poly(isobutene), poly(trimethyl glutarate), polyainhydides, polyorthoesters, polystyrene, polyurethane, polypropylene, polymethacrylate, polytetrafluoroethylene, and other known polymers which will bind with the dye and infrared absorbing compound to keep them in close proximity:
The colored compositions that result in tissue markings of the eurrent invention may also be in the form of an encapsulant, used to encapsulate, -entrap, encase, complex, or, in general, incorporate the at least one colored compound and the at least one infrared absorbing compound. The encapsulating materials are chosen to be biocompatible with the. body and are generally transparent to visible radiation so that the color of the dye is efficiently presented. The encapsulant is also chosen to be essentially transparent to infrared radiation so that, during the process for rendering the tissue marking colorless, most or all of the applied infrared radiation reaches the infrared absorbing compound.
They can be designed to remain indefinitely in the tissue, for example, by making the tissue marking large enough to resist elimination from the tissue or by designing-the vehicle to interact with the tissue, such as, for example, through chemical bonding.
Vehicles useful in the current invention include organic polymers, waxes,-ethylene glycols, silicon based polymers, hydrogels, liposomes and combinations thereof.

Examples.of suitable organic polymers include polyacrylates=, polyurethanes, polyamides, polyimides, polyesters, polyethylene oxides, and combinations thereof.
Examples of vehicles and methods of preparation useful to the current invention are disclosed in U.S:
Patent No. 6,013,122. It should be noted that.the encapsulant is designed to remain:intact during the process for rendering the tissue marking colorless..
The colored compositions that result in tissue markings tissue markings of the current invention as described above can be introduced either as an admixture with a liquid carrier, an admixture with binder materials, as an encapsulant, or combinations thereof, into the tissue by a procedure that consists of piercing the tissue with needles or similar instruments to introduce the tissue marking by an alternating pressure-suction action. Typical processes use tattooing equipment well known to the art, for example, an electromagnetic coil machine, a rotary application machine or a maciual device. Non-invasive methods are also useful for application of the current inventive-tissue markings, such as, for example, ultrasonic techniques which cause tissue to become more porous; as described in U.S. Patent No. 5,445,611.
It should be noted that the tissue marking compositions of this invention are not.
required to be encapsulated for penrnanence. For such cases, by controlling the size of the colored composition particles, one can ensure permanence. Minute particles (less than 0.15 microns) will have a higher probability of removal by the biological processes of the body. Hence, by keeping the colored composition particles about in the range of 0.15 to 6 microns, a sufficient number of the particles will be ietained to create a permanent visible tissue marking. For example, for the case of a skin marking, this implies that a sufficient number of the particles will be either engulfed by the phagocytic skin cells (such as fibroblasts and macrophages) or retained in the extracellular matrix.
The tissue markings of the current invention can be "removed", that is, become colorless by application of infrared radiation. Thesource of infrared radiation may generally be an infrared laser. Both the infrared radiation and the infrared absorbing compound are chosen so that the infrared radiation is optimally absorbed by the inventive tissue marking. The optimal spectral range for the laser is 800 - 1800 nm.
This spectral range is suitable for this application since the body has minimal absorbing materials in this range. Energy absorbing substances present in the body are water, that absorbs at 1800 nm and greater, melanin which broadly absorbs up to about 1100 nm, but absorbs far less energy at wavelengths over 800 nm; and oxyhemoglobin which has a maximum absorbance in the range of 400 to 600 nm. Short, intense pulses of light are absorbed specifically by the infrared absorbing coinpound of the tissue marking of the-current invention with minimal absorption by the surrounding tissue. Again, since.the surrounding tissue is transparent to the infrared radiation, damage to the surrounding tissue can be minimized or eliminated. When the tissue marking is introduced as encapsulated ink, the method of rendering the ink colorless occurs without the disruption of the encapsulant, such that the fragments of the once colored compound, the infrared absorbing compound and any other materials present remain encapsulated in the skin.
It should be noted that since the infrared absorbing compound, the infrared radiation source and the thermally labile substituent all chosen independently from the colored compound.of the inventive ink, the method of "removal" or rendering the ink colorless is independent of the colored compound. As a result, using the ctirrent method, tissue markings of any color can be rendered colorless using a single application of a single infrared radiation source, such as a laser:

Examples Example 1(Colored comnound to colorless compound through thermal fragmentation of the colored compound) The magenta compound of Figure 2A was admixed with acetone and placed onto a glass slide and the acetone was allowed to evaporate at ambient conditions. The slide was placed onto a hot plate heated to approximately 250 C. The magenta color of the dye disappeared within one minute. The slide was cooled and rinsed with acetone into a small beaker to collect the material on the slide. Performing TLC on silica gel of the materials rinsed from the glass slide confirmed that the magenta compound from Figure 2A
had been completely converted to the cyclized compound in Figure2B. (Rf of the, magenta compound of Figure 2A = 0.2, the Rf of the compound of Figure 2B = 0.9).

Example 2 (Colored composition containine colored compound and binder to colorless composition through thermal fragmentation of the colored compound) 223 mgs of the magenta compound of Figure 2A was, admixed with 5.13 gms of acetone.
2.53 gms of this admix was added to 10.0 gms of a 15% w/w admix-of PMMA
(polymethylmethacrylate). The admix thus contains 0.11 gms of-the magenta compound of Figure 2A and 1.5 gms of PMMA. Three drawdowns of this admix were made on PET (polyethylene-terephthalate) film using #12, #15 and #20 wire-wound coating rods to give three different coating thicknesses. The PET films were allowed to dry at ambient conditions. The three strips of coated PET film were placed on a hot plate.heated to approximately 250 C. The magenta color of each film disappeared within 2 minutes.
Example 3 (Thermal fragmentation.of the maaenta dye with application of Infrared radiation) 100 mgs of IR 1065A (an infrared absorbing compound obtained from ADS, Inc of Quebec, Canada) was admixed with 1.06 gms of acetone. 1.68 gms of an admix of 223 mgs of the magenta compound of Figure 2A and 5.13 gms of acetone was added to 6.1 gms of a 15% admixture of PMMA in acetone. The acetone admix of the IR dye was added to the PMMA/magenta admix. Three drawdowns of this admix were made on white reflective PET film using #12, #15 and #20 wire-wound coating rods to give three different coating thicknesses. The PET films were allowed to dry at ambient conditions.
Reflection density measurements with a Greytag MacBeth Spectrolino Densitiometer gave a reading of 1.99 logio density at a wavelength of 570 nm. A strip of the #12 bar coating was placed approximately 2.5 inches in front of a collimated IR laser eniitting at 980+/- 15 nm. A 1 second pulse from the laser resulted in a uniformly colorless circie of approximately 3/16 inches in diameter in the magenta coating. The reflection density measurement after exposure to the IR laser was 0.26 logio density units. It should be noted that the base density ( the reflectance of the.white reflective PET film was 0.06 logio density units). Hence the laser exposure lowered the reflection density from 1.93 (1.99 minus 0.06) toØ20 (0.26 minus 0:06), a reductiori of approxiniately 90%. It should also be noted that.a black density measurement of the sample afterexposure using the same densitometer gave a reading of 0.261ogio density units that is 0.201og10 density units over the base.

Claims

1. A tissue marking comprising at least one colored compound comprising a thermally activatable fragmentation group and at least one infrared absorbing compound, wherein the at least one colored compound is capable of being rendered colorless by unimolecular fragmentation of the thermally activatable fragmentation group when the tissue marking is non-imagewise exposed by a source of infrared radiation.

2. The tissue marking of claim 1 wherein the at least one colored compound comprises the form of:
D-Sp-Nu-FG
wherein D comprises a chromophore, Sp comprises an optional spacer group connecting the colored compound and a nucleophile Nu, Nu comprises a substituted or unsubstituted nucleophilic group and FG comprises the thermally activatable fragmentation.

3. The tissue marking of claim 2, wherein the chromophore D comprises a substituted or unsubstituted triarylmethane, a xanthene, a rhodamine, a fluoran, a azocarbocyanine, a benzylidene, a thiazine, an acridine, an aminoanthraquinone or other chromophore; wherein the optional spacer group Sp comprises substituted or unsubstituted alkyl groups, cycloaliphatic groups, sulfur or phosphorus containing groups or their oxides, or other spacing groups; wherein nucleophile group Nu comprises substituted or unsubstituted, oxygen, sulfur, phosphorus, carbon, selenium, nitrogen or silicon, or oxides thereof, or other substituted or unsubstituted nucleophilic group; and wherein the thermally activatable fragmentation group FG
comprises substituted or unsubstituted carbonates, carbamates, esters, lactams, lactones, amides, imides, oximes, sulfonates, sulfinates, sulfenates, phosphates, phosphonates, or --- R2C-RHC-Y, wherein R is the same or different and comprises substituted or unsubstituted alkyl, aryl, or aralkyl groups and wherein Y
comprises cyano, nitro, a sulfoxide, a carbonyl containing compound, a sulfonamide or other electron-withdrawing substituent that is capable of fragmenting when a sufficient amount of thermal energy is applied.

4. The tissue marking of any one of claims 1-3, wherein the at least one infrared absorbing compound comprises a cyanine, phthalocyanine, squarylium, azulenium, indophenol, naphthoquinone, or anthraquinone.

5. The tissue marking of claim 4, wherein the infrared absorbing compound is chosen such that its absorption is such that the surrounding tissue is substantially transparent to the radiation being absorbed by the compound and wherein the source of infrared radiation is a laser.

6. The tissue marking of claim 4, wherein the at least one colored compound further comprises one or more ballast groups attached to the dye, the thermally activatable fragmentation group or both.

7. The tissue marking of claim 4, further comprising a biocompatible binder material.

8. The tissue marking of claim.7, wherein the infrared absorbing compound is chosen such that its absorption is such that the surrounding tissue is substantially transparent to the radiation being absorbed by the compound and wherein the source of infrared radiation is a laser.

9. The tissue marking of claim 4, further comprising a biocompatible polymeric encapsulant to encapsulate the at least one colored compound and the at least one infrared absorbing compound.

10. The tissue marking of claim 9, wherein the infrared absorbing compound is chosen such that the surrounding tissue is substantially transparent to the radiation being absorbed by the compound.