CA2763269C - Methods and apparatus for perfusion, diagnosis, storage and/or transport of an organ or tissue - Google Patents

Methods and apparatus for perfusion, diagnosis, storage and/or transport of an organ or tissue Download PDF

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Publication number
CA2763269C
CA2763269C CA2763269A CA2763269A CA2763269C CA 2763269 C CA2763269 C CA 2763269C CA 2763269 A CA2763269 A CA 2763269A CA 2763269 A CA2763269 A CA 2763269A CA 2763269 C CA2763269 C CA 2763269C
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Prior art keywords
portable housing
compartment
organ
heat transfer
transfer surfaces
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CA2763269A
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French (fr)
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CA2763269A1 (en
Inventor
David Walter Wright
John Brassil
Douglas Schein
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Organ Recovery Systems Inc
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Organ Recovery Systems Inc
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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0236Mechanical aspects
    • A01N1/0263Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
    • A01N1/0273Transport containers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0236Mechanical aspects
    • A01N1/0242Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
    • A01N1/0247Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components for perfusion, i.e. for circulating fluid through organs, blood vessels or other living parts
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/809Incubators or racks or holders for culture plates or containers

Abstract

An organ perfusion apparatus and method monitor, sustain and/or restore viability of organs and preserve organs for storage and/or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The apparatus and methods include the organ cassette with one or more openings configured to allow tubing to pass through the openings and be connected to the organ or tissue within the cassette, and including a pressure control device to allow pressure inside the portable housing to be varied. The apparatus and methods further include the cassette with heat transfer surfaces arranged to transfer heat between a cooling source in said transporter and the heat transfer surfaces. The apparatus and methods further include a tube frame removably connected to the cassette and configured to hold a plurality of tubes in a position to be connected to tubes in the portable housing.

Description

= . ' METHODS AND APPARATUS FOR PERFUSION, DIAGNOSIS, STORAGE AND/OR
TRANSPORT OF AN ORGAN OR TISSUE
BACKGROUND OF THE INVENTION
100011 The invention relates to apparatus and methods for perfusing one or more organs, tissues or the like (hereinafter generally referred to as organs) to monitor, sustain and/or restore viability of the organs. This invention further relates to transportation and/or storage of the organs in a housing configured to allow pressure of the housing to be varied. This invention further relates to allowing effective heat transfer to or from the contents of a housing. This invention further relates to providing a tube frame configured to hold a plurality of tubes and removably connected to the housing.
[0002] Preservation of organs by machine perfusion has been accomplished at hypothermic temperatures with or without computer control with crystalloid perfusates and without oxygenation. See, for example, U.S. Patents Nos. 5,149,321, 5,395,314, 5,584,804, 5,709,654, 5,752,929 and 5,827,222 to Klatz et al. Hypothermic temperatures provide a decrease in organ metabolism, lower energy requirements, delay depletion of high energy phosphate reserves and accumulation of lactic acid and retard morphological and functional deterioration associated with disruption of blood supply.
[0003] Ideally organs would be procured in a manner that limits their warm ischemia time to essentially zero. Unfortunately, in reality, many organs, especially from non-beating heart donors, are procured after extended warm ischernia time periods (i.e., 45 minutes or more). The machine perfusion of these organs at low temperature has demonstrated significant improvement (Transpl Int 1996 Daemen). Further, prior art teaches that the low temperature machine perfusion of organs is preferred at low pressures (Transpl. Int 1996 Yland) with roller or diaphragm pumps delivering the perfusate at a controlled pressure. Numerous control circuits and pumping configurations have been utilized to achieve this objective and to machine perfuse organs in general.
See, for example, U.S. Patents Nos. 5,338,662 and 5,494,822 to Sadri; U.S.
Patent No.
4,745,759 to Bauer et al.; U.S. Patents Nos. 5,217,860 and 5,472,876 to Fahy et al.; U.S.
Patent No. 5,051,352 to Martindale et al.; U.S. Patent No. 3,995,444 to Clark et al.; U.S.
Patent No. 4,629,686 to Greenberg; U.S. Patents Nos. 3,738, 914 and 3,892,628 to Thorne et al.; U.S. Patents Nos. 5,285,657 and 5,476,763 to Bacchi et al.;
U.S. Patent No. 5,157,930 to McGhee et al.; and U.S Patent No. 5,141,847 to Sugimachi et al.

[0004] WO 88/05261 to Owen discloses an organ perfusion system including an organ chamber that is supplied with an emulsion fluid or physiological electrolyte that is transported through a perfusion system. The chamber contains a synthetic sac to hold the organ. Perfusate enters the organ through a catheter inserted into an artery.
The perfusate is provided by two independent fluid sources, each of which includes two reservoirs.
SUMMARY OF THE INVENTION
[0005] The present invention focuses on avoiding damage to an organ during perfusion while monitoring, sustaining and/or restoring the viability of the organ and preserving the organ for storage and/or transport. The invention is directed to apparatus and methods for pedusing an organ to monitor, sustain and/or restore the viability of the organ and/or for transporting and/or storing the organ.
[0006] In perfusion, gross organ perfusion pressure may be provided by a pneumatically pressurized medical fluid reservoir controlled in response to a sensor disposed in an end of tubing placed in the organ, which may be used in combination with a stepping motor/cam valve or pinch valve which provides for perfusion pressure fine tuning, prevents over pressurization and/or provides emergency flow cut-off. Alternatively, the organ may be perfused directly from a pump, such as a roller pump or a peristaltic pump, with proper pump control and/or sufficiently fail-safe controllers to prevent over pressurization of the organ, especially as a result of a system malfunction. Substantially eliminating over pressurization prevents and/or reduces damage to the vascular endothelial lining and to the organ tissue in general.
[0007] Apparatus of the invention may be used for various organs, such as the kidneys, and may be adapted to more complex organs, such as the liver, having multiple vasculature structures, for example, the hepatic and portal vasculatures of the liver.
[0008] An organ diagnostic apparatus may also be provided to produce diagnostic data such as an organ viability index. The organ diagnostic apparatus includes features of an organ perfusion apparatus, such as sensors and temperature controllers, as well as cassette interface features, and provides analysis of input and output fluids in a perfusion. system.
Typically, the organ diagnostic apparatus is a simplified perfusion apparatus providing diagnostic data in a single pass, in-line perfusion.
[0009] Embodiments of the invention also provide an organ cassette which allows an organ to be easily and safely moved between apparatus for perfusing, storing, analyzing and/or transporting the organ. The organ cassette may be configured to provide uninterrupted sterile conditions and efficient heat transfer during transport, recovery, analysis and storage, including transition between the transporter, perfusion apparatus and organ diagnostic apparatus, and/or other apparatus.
100101 Embodiments of this invention also provide an organ transporter which allows for transportation of an organ, particularly over long distances. The organ transporter may include features of an organ perfusion apparatus, such as sensors and temperature controllers, as well as cassette interface features.
[00111 Embodiments of this invention provide a pressure control device with the cassette to allow a pressure inside the cassette to be varied.
[0012] Embodiments of this invention provide a pressure control valve and/or a closable vent to control the pressure inside the cassette.
[00131 Embodiments of this invention provide one or more membranes to allow the pressure inside the cassette to be varied.
[00141 Embodiments of the invention provide for one or more of the various types of pressure control devices to be used in conjunction for controlling the pressure inside the cassette.
[0015] Embodiments of this invention provide one or more lids with the cassette and the pressure control device(s) are located in the one or more lids.
[00161 Embodiments of this invention provide filters associated with the pressure control devices to control the entry of contaminants into the cassette.
[00171 Embodiments of this invention provide a cooling source in the transporter.
[00181 Embodiments of this invention provide the cassette and a compartment of the transporter with one or more heat transfer surfaces which contact and allow effective heat transfer to or from the contents of the cassette.
[0019] Embodiments of this invention provide the cassette and the conipaitinent of the transporter with substantially complementary mating configurations.
[0020] Embodiments of this invention provide for planar and non-planar heat transferring surfaces.
[0021) Embodiments of this invention provide a tube frame configured to hold a plurality of tubes and removably connected to the cassette.
100221 Embodiments of this invention provide a tube frame configured to hold the plurality of tubes in a position to be connected to a plurality of tubes in the cassette.

[0023] Embodiments of this invention provide for the tube frame to be connectible to any one of a cassette, a transporter, an organ perfusion apparatus, or an organ diagnostic device.
[0024] Embodiments of this invention provide for a sensor to detect a proper connection between the tube frame and the device to which it is connected.
[0024a] In accordance with an aspect of the present invention there is provided an apparatus for holding an organ or a tissue for at least one of perfusion, storage, diagnosis and transport of the organ or tissue, comprising: an organ or tissue transporter being configured to perfuse an organ or a tissue; a portable housing having one or more heat transfer surfaces; a compartment within the transporter having one or more heat transfer surfaces arranged on an outer surface of the compartment to transfer heat between a cooling source contained within the compartment and at least a part of one of the one or more heat transfer surfaces of the portable housing, the compartment being liquid-tight and preventing contact between a liquid contained within the compartment and the portable housing; and an organ or tissue supporting surface configured to support the organ or tissue within said portable housing, wherein the portable housing is configured to be received by the transporter and wherein at least a portion of at least one of the one or more heat transfer surfaces of the compartment is in contact with at least a portion of at least one of the one or more heat transfer surfaces of the portable housing to allow effective heat transfer to or from the contents of the portable housing.
[0024b1 In accordance with a further aspect of the present invention there is provided a method of transporting an organ or a tissue, comprising: placing the organ or tissue in a portable housing, the portable housing having one or more heat transfer surfaces;
placing the portable housing containing the organ or tissue in a compartment of a transporter configured to receive the portable housing and configured to perfuse the organ or tissue, the compartment having one or more heat transfer surfaces arranged on an outer surface of the compartment, the compartment being liquid-tight and preventing contact between a liquid contained within the compartment and the portable housing, wherein at least a portion of at least one of the one or more heat transfer surfaces of the compartment is in contact with at least a portion of at least one of the one or more heat transfer surfaces of the portable housing; transferring heat between a cooling source contained within the compartment, via at least a part of one of the one or more heat transfer surfaces of the compartment, to or from the contents of the portable housing;
and transporting the organ in said portable housing in said transporter.

4a 10024c] In accordance with a further aspect of the present invention there is provided a system for holding an organ or tissue for transport of the organ or tissue, comprising: an organ or tissue transporter; a portable housing having one or more heat transfer surfaces; a compartment within the transporter having one or more heat transfer surfaces arranged on an outer surface of the compartment to transfer heat between a cooling source contained within the compartment and at least a part of one of the one or more heat transfer surfaces of the portable housing, the compartment being liquid-tight and preventing contact between a liquid contained within the compartment and the portable housing;
and an organ or tissue supporting surface configured to support an organ or tissue within said portable housing; wherein the portable housing is configured to be received by the transporter and wherein at least a portion of at least one of the one or more heat transfer surfaces of the compartment is in contact with at least a portion of at least one of the one or more heat transfer surfaces of the portable housing to allow effective heat transfer to or from the contents of the portable housing, wherein the portable housing includes openings configured to allow tubing to pass through said openings and be connected to the organ, the tubing being configured to pass through the openings when the portable housing is mated with the compartment and being configured to be mated with the portable housing when the portable housing is removed from the compartment, and wherein the portable housing includes a handle that is accessible when the portable housing is mated with the compai __ tment.
[0024d] In accordance with a further aspect of the present invention there is provided an apparatus for holding an organ or a tissue for at least one of perfusion, storage, diagnosis and transport of the organ or tissue, comprising: an organ or tissue transporter; a portable housing having one or more heat transfer surfaces; a compartment within the transporter having one or more heat transfer surfaces arranged on an outer surface of the compartment to transfer heat between a cooling source contained within the compartment and at least a part of one of the one or more heat transfer surfaces of the portable housing, the compai tment being liquid-tight and preventing contact between a liquid contained within the compat nnent and the portable housing; and an organ or tissue supporting surface configured to support the organ or tissue within said portable housing, wherein the portable housing is configured to be received by the transporter, wherein at least a portion of at least one of the one or more heat transfer surfaces of the compartment is in contact with at least a portion of at least one of the one or more heat transfer surfaces of the portable housing to allow effective heat transfer to or from the contents of the portable housing, wherein the portable housing includes openings configured to allow tubing to pass through said openings and be connected to the organ or tissue, and wherein the tubing can pass through the openings when the portable housing is mated with the compai __ tment.

4b [0024e] In accordance with a further aspect of the present invention there is provided a method of transporting an organ or a tissue, comprising: placing the organ or tissue in a portable housing, the portable housing having one or more heat transfer surfaces; placing the portable housing containing the organ or tissue in a compartment of a transporter configured to receive the portable housing, the compartment having one or more heat transfer surfaces arranged on an outer surface of the compartment, the compartment being liquid-tight and preventing contact between a liquid contained within the compartment and the portable housing, wherein at least a portion of at least one of the one or more heat transfer surfaces of the compartment is in contact with at least a portion of at least one of the one or more heat transfer surfaces of the portable housing;
transferring heat between a cooling source contained within the compartment, via at least a part of one of the one or more heat transfer surfaces of the compartment, to or from the contents of the portable housing; and transporting the organ in said portable housing in said transporter, wherein the portable housing includes openings configured to allow tubing to pass through said openings and be connected to the organ, and wherein the tubing can pass through the openings when the portable housing is mated with the compartment.
[0025] These and other features and advantages of this invention are described in, or are apparent from, the following detailed description of various exemplary embodiments of systems and methods according to this invention.
BRIEF DESCRIPTION OF THE DRAWINGS
[0026] The above and other aspects and advantages of the invention will become apparent from the following detailed description of embodiments when taken in conjunction with the accompanying drawings, in which:
[0027] Fig. 1 is an organ perfusion apparatus according to the invention;
[0028] Fig. 2 is a schematic diagram of an apparatus of Figure 1;
[0029] Fig. 3 is a diagram of the electronics of the apparatus of Figure 2;
[00301 Figs. 4A-4D show perspective views of various embodiments of an organ cassette according to the invention;
[0031] Fig. 5 is a schematic diagram of an organ perfusion apparatus configured to simultaneously perfuse multiple organs;
[0032] Figs. 6A and 6B show an embodiment of an organ cassette of the present invention;
[0033] Fig. 7 shows an exterior perspective view of an organ transporter according 4c to the present invention;
[0034] Fig. 8 shows a cross section view of an organ transporter of Figure 7;
[0035] Fig. 9 shows an alternative cross-section view of an organ transporter of Figure 7;
[0036] Fig. 10 shows the mating of a cassette with a compartment according to the present invention;
[0037] Fig. 11 shows a tube frame with a tube set according to the present invention; and [0038] Fig. 12 shows a tube frame connected to a cassette according to the present invention.
DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS

[0039] For a general understanding of the features of the invention, reference is made to the drawings. In the drawings, like reference numerals have been used throughout to designate like elements.
[00401 The invention is described herein largely in the context of apparatus and methods involved in transport, storage, perfusion and diagnosis of tissues and organs.
However, the inventive apparatus and methods have many other applications, and thus the various inventive structures, devices, apparatus and methods described herein should not be construed to be limited to, particular contexts of use. Various features of the disclosed invention are particularly suitable for use in the context of, and in conjunction and/or connection with the features of the apparatus and methods disclosed in U.S.
patent application no. 09/645,525õ
[00411 Figure 1 shows an organ perfusion apparatus 1 according to the invention.
Figure 2 is a schematic illustration of the apparatus of Fig. 1. The apparatus 1 is preferably at least partially microprocessor controlled, and pneumatically actuated. A
microprocessor 150 connection to the sensors, valves, thermoelectric units and pumps of the apparatus 1 is schematically shown in Fig. 3. Microprocessor 150 and apparatus 1 may be configured to and are preferably capable of further being connected to a computer network to provide data sharing, for example across a local area network or across the Internet_ [0042] The organ perfusion apparatus 1 is preferably capable of perfusing one or more organs simultaneously, at both normothermic and hypothermic temperatures (hereinafter, normothermic and hypothermic perfusion modes). All medical fluid contact surfaces are preferably formed of or coated with materials compatible with the medical fluid used, more preferably non-thrombogenic materials. As shown in Fig. 1, the apparatus 1 may include a housing 2 which includes flout cover 4, which is preferably translucent, and a reservoir access door 3. The apparatus preferably has one or more control and display areas 5a, 5b, 5c, 5d for monitoring and controlling perfusion.
[0043] As schematically shown in Fig. 2, enclosed within the housing 2 is a reservoir 10 which preferably includes three reservoir tanks 15a, 15b, 17. Two of the reservoir tanks 15a, 15b are preferably standard one liter infusion bags, each with a respective pressure cuff 16a, 16b. A pressure source 20 can be provided for pressurizing the pressure cuffs 16a, 16b. The pressure source 20 is preferably pneumatic and may be an on board compressor unit 21 supplying at least 10 LPM external cuff activation via gas tubes 26,26a,26b, as shown in Fig. 2. The invention, however, is not limited to use of an on board compressor unit as any adequate pressure source can be employed, for example, a compressed gas (e.g., air, CO2, oxygen, nitrogen, etc.) tank (not shown) preferably with a tank volume of 1.5 liters at 100 psi or greater for internal pressurization.
Alternatively, an internally pressurized reservoir tank (not shown) may be used. Reservoir tanks 15a, 15b, 17 may, in embodiments, be bottles or other suitably rigid reservoirs that can supply perfusate by gravity or can be pressurized by compressed gas.
[0044] Gas valves 22-23 may be provided on the gas tube 26 to allow for control of the pressure provided by the onboard compressor unit 21. Anti-back flow valves 24a, 24b may be provided respectively on the gas tubes 26a, 26b. Pressure sensors P5, P6 may be provided respectively on the gas tubes 26a, 26b to relay conditions therein to the microprocessor 150, shown in Fig. 3. Perfusion, diagnostic and/or transporter apparatus may be provided with sensors to monitor perfusion fluid pressure and flow in the particular apparatus to detect faults in the particular apparatus, such as pressure elevated above a suitable level for maintenance of the organ. Gas valves GVI and GV2 may be provided to release pressure from the cuffs 16a, 16b. One or both of gas valves GVi and GV2 may be vented to the atmosphere. Gas valve GV4 in communication with reservoir tanks 15a, 15b via tubing 18a, 18b may be provided to vent air from the reservoir tanks 15a, 15b through tubing 18. Tubing 18, 18a, 18b, 26, 26a and/or 26b may be configured with filters and/or check valves to prevent biological materials from entering the tubing or from proceeding further along the fluid path. The check valves and/or filters may be used to prevent biological materials from leaving one organ perfusion tubeset and being transferred to the tubeset of a subsequent organ in a multiple organ perfusion configuration. The check valves and/or filters may also be used to prevent biological materials, such as bacteria and viruses, from being transferred from organ to organ in subsequent uses of the perfusion apparatus in the event that such biological materials remain in the perfusion apparatus after use. The check valves and/or filters may be provided to prevent contamination problems associated with reflux in the gas and/or vent lines. For example, the valves may be configured as anti-reflux valves to prevent reflux. The third reservoir tank 17 is preferably pressurized by pressure released from one of the pressure cuffs via gas valve GV2.
[0045] The medical fluid may be blood or a synthetic fluid and may, for example, be a simple crystalloid solution, or may be augmented with an appropriate oxygen carrier.
The oxygen carrier may, for example, be washed, stabilized red blood cells, cross-linked hemoglobin, pegolated hemoglobin or fluorocarbon based emulsions. The medical fluid may also contain antioxidants known to reduce peroxidation or free radical damage in the physiological environment and specific agents known to aid in tissue protection. An oxygenated (e.g., cross-linked hemoglobin-based bicarbonate) solution is preferred for a normothermic mode while a non-oxygenated (e.g., simple crystalloid solution preferably augmented with antioxidants) solution is preferred for a hypothermic mode. The specific medical fluids used in both the normothermic and hypothermic modes may be designed or selected to reduce or prevent the washing away of or damage to the vascular endothelial lining of the organ. For a hypothermic perfusion mode, as well as for flush and/or static storage, a preferred solution is the solution disclosed in U.S. Patent No.
6,492,103, the entire disclosure of which is incorporated herein by reference. Examples of additives which may be used in perfusion solutions for the present invention are also disclosed in U.S. Patent No.
6,046,046 to Hassanein, Of course, other suitable solutions and materials may be used, as is known in the art.
[0046] The medical fluid within reservoir 10 is preferably brought to a predetermined temperature by a first thermoelectric unit 30a in heat transfer communication with the reservoir 10. A temperature sensor T3 relays the temperature within the reservoir 10 to the microprocessor 150, which adjusts the thermoelectric unit 30a to maintain a desired temperature within the reservoir 10 and/or displays the temperature on a control and display areas Sa for manual adjustment. Alternatively or in addition, and preferably where the organ perfusion device is going to be transported, the medical fluid within the hypothermic perfusion fluid reservoir can be cooled utilizing a cryogenic fluid heat exchanger apparatus such as that disclosed in filed U.S. Patent No. 6,014,864, which is hereby incorporated by reference.
[0047] An organ chamber 40 is provided which supports a cassette 65, as shown in Fig. 2, which holds an organ to be perfused, or a plurality of cassettes 65, as shown in Fig. 5, preferably disposed one adjacent the other. Various embodiments of the cassette 65 are shown in Figs. 4A-4D. The cassette 65 is preferably formed of a material that is light but durable so that the cassette 65 is highly portable. The material may also be transparent to allow visual inspection of the organ.
[0048] Fig. 4A shows a cassette 65 which holds an organ 60 to be perfused.
Various embodiments of such a cassette 65 are shown in Figs. 4A-4D, 6A, 6B, 10 and 12.
The cassette 65 is preferably formed of a material that is light but durable so that the cassette 65 is highly portable. The material may also be transparent to allow visual inspection of the organ.

[0049] Preferably the cassette 65 includes side walls 67a, a bottom wall 67b and an organ supporting surface 66, which is preferably formed of a porous, perforated or mesh material to allow fluids to pass there through. The cassette 65 may also include a top 67d and may be provided with an opening(s) 63 for tubing (see, for example, Fig. 4D).
The opening(s) 63 may include seals 63a (e.g., septum seals or o-ring seals) and optionally be provided with plugs (not shown) to prevent contamination of the organ and maintain a sterile environment. Additionally, the cassette 65 may be provided with tubing for connection to an organ and/or to remove medical fluid from the organ bath, and a connection device(s) 64 for connecting the tubing to, for example, tubing 50c, 81, 82, 91 and/or 132, (see, for example, Fig. 4D) of an organ storage, transporter, perfusion and/or diagnostic apparatus.
100501 The cassette 65, and/or the organ support, opening(s), tubing(s) and/or connections(s), may be specifically tailored to the type of organ and/or size of organ to be perfused. Flanges 67c of the side support walls 67a can be used to support the cassette 65 disposed in an organ storage, transporter, perfusion and/or diagnostic apparatus. The cassette 65 may further include a handle 68 which allows the cassette 65 to be easily handled, as shown, for example, in Figs. 4C and 4D. Each cassette 65 may also be provided with its own mechanism (e.g., stepping motor/cam valve 75 (for example, in the handle portion 68, as shown in Fig. 4C)) for fine tuning the pressure of medical fluid perfused into the organ 60 disposed therein, as discussed in more detail below. Alternatively, pressure may, in embodiments, be controlled by way of a pneumatic chamber, such as an individual pneumatic chamber for each organ (not shown), or by any suitable variable valve such as a rotary screw valve or a helical screw valve.
[0051] Cassette 65 may be provided with a closeable and/or filtered vent 61 (see, for example, Fig. 4D). Vent 61 preferably includes a filter device, and provides for control and/or equalization of pressure within the cassette without contamination of the contents of the cassette. For example, organs are frequently transported by aircraft, in which pressure changes are the norm. Even ground transportation can involve pressure changes as motor vehicles pass through tunnels, over mountains, etc. It is often desirable to provide for pressure equalization of the cassette under such circumstances. However, free flow of air to achieve pressure equalization might introduce contaminants into the cassette.
Thus, a filtering vent 61 is preferably provided to allow the air flow without permitting introduction of contaminants into the cassette.
100521 The filter preferably will let clean air pass in both directions but will not allow dirt, dust, liquids and other contaminants to pass. The pore size in the filters can be any =

size desired and can be small enough to prevent bacteria from passing. A
pressure control valve can optionally be associated with vent 61 as well. Such a valve may be configured or controlled to restrict the rate at which external pressure changes are transmitted to the inside of the cassette, or even to prevent pressure increases and/or decreases, as desired.
[0053] Figs. 6A-6B show an alternative embodiment of cassette 65. The cassette is a portable device and is provided with a lid, preferably two lids, an inner lid 410 and an outer lid 420. As such, the one or more lids 410 and 420 can create a substantially airtight seal with the cassette 65. This air tight seal can create a pressure difference between the inside and outside of cassette 65. Pressure sensors that control perfusion of the organ may be referenced to the atmospheric pressure. In such embodiments, it is desirable that the air space around the organ in cassette 65 is maintained at atmospheric pressure.
[0054] Accordingly, the cassette may also include one or more devices for controlling the pressure. The devices for controlling pressure can be an active or passive device such as a valve or membrane. Membranes 415 and 425, for example, can be located in the inner lid 410 and outer lid 420, respectively. It should be appreciated that any number of membranes can be located in the cassette (including its lid(s)) without departing from the spirit and scope of the invention. The membranes 415 and 425 are preferably hydrophobic membranes which help maintain an equal pressure between the inside and the outside of the cassette. A pressure control valve can optionally be associated with membranes 415 and 425.
Such a pressure control valve may be configured or controlled to restrict the rate at which external pressure changes are transmitted to the inside of the cassette, or even to prevent pressure increases and/or decreases, as desired.
[0055] The membranes 415 and 425, if sufficiently flexible, can be impermeable or substantially impermeable. Alternatively, they may include filters that will let clean air pass in both directions, however, the membranes 415 and 425 will not allow dirt, dust, liquids and other contaminants to pass. The pore size in the filters can be any size desired, and preferably, the pore size of the membranes 415 and 425 can be small enough to prevent bacteria from passing. The actions of the membranes 415 and 425 and corresponding filters help maintain the sterility of the system.
[0056] Preferably, cassette 65 is made of a sufficiently durable material that it can withstand penetration and harsh impact. The lids 410 and 420 may be removable or may be hinged or otherwise connected to the body of cassette 65. Clasp 405, for example, may provide a mechanism to secure lids 410 and 420 to the top of cassette 65.
Clasp 405 may additionally be configured with a lock to provide further security and stability. A biopsy and/or venting port 430 may additionally be included in inner lid 410 or both inner lid 410 and outer lid 420. Port 430 may provide access to the organ to allow for additional diagnosis of the organ with minimal disturbance of the organ. Cassette 65 may also have an overflow trough 440 (shown in Fig. 6B as a channel present in the top of cassette 65).
When lids 410 and 420 are secured on cassette 65, overflow trough 440 provides a region that is easy to check to determine if the inner seal is leaking. Perfusate may be poured into and out of cassette 65 and may be drained from cassette 65 through a stopcock or removable plug.
100571 In Fig. 6A, cassette 65 is shown with tubeset 400. Tubeset 400 can be connected to perfusion apparatus I or to an organ transporter or an organ diagnostic apparatus, and allows cassette 65 to be moved between various apparatus without jeopardizing the sterility of the interior of cassette 65. Preferably, cassette 65 is made of a sufficiently durable material that it can withstand penetration and harsh impact. As shown in Fig. 6A, the tube set may be connected to a bubble trap device BT. A preferred such device is described in detail in a U.S. provisional patent application filed simultaneously herewith entitled "Device for separating bubbles from a liquid path" (attorney docket no. 115624).
[0058] Cassette 65 and/or its lid(s) may be constructed of an optically transparent material to allow for viewing of the interior of cassette 65 and monitoring of the organ and to allow for video images or photographs to be taken of the organ. A perfusion apparatus or cassette 65 may be wired and fitted with a video camera or a photographic camera, digital or otherwise, to record the progress and status of the organ. Captured images may be made available over a computer network such as a local area network or the intemet to provide for additional data analysis and remote monitoring. Cassette 65 may also be provided with a tag that would signal, e.g., through a bar code, magnetism, radio frequency, or other means, the location of the cassette, that the cassette is in the apparatus, and/or the identity of the organ to perfusion, storage, diagnostic and/or transport apparatus. Cassette 65 may be sterile packaged and/or may be packaged or sold as a single-use disposable cassette, such as in a peel-open pouch. A single-use package containing cassette 65 may also include tubeset 400 and/or tube frame 200, discussed further below.
[0(159] Cassette 65 is preferably configured such that it may be removed from an organ perfusion apparatus and transported to another organ perfusion and/or diagnostic apparatus in a portable transporter apparatus as described herein or, for example, a conventional cooler or a portable container such as that disclosed in U.S.
Patent No.
6,209,343, or U.S. Patent No. 5,586,438, [0060] In various exemplary embodiments according to this invention, when transported, the organ may be disposed on the organ supporting surface 66 and the cassette 65 may be enclosed in a preferably sterile bag 69, as shown, for example, in Fig.
4A. When the organ is perf-used with medical fluid, effluent medical fluid collects in the bag 69 to form an organ bath. Alternatively, cassette 65 can be formed with a fluid tight lower portion in which effluent medical fluid may collect, or effluent medical fluid may collect in another compartment of an organ storage, transporter, perfusion and/or diagnostic apparatus, to form an organ bath. In either case, the bag 69 would preferably be removed prior to inserting the cassette into an organ storage, transporter, perfusion and/or diagnostic apparatus. Further, where a plurality of organs are to be perfused, multiple organ compartments may be provided. Alternatively, cassette 65 can be transported in the cassette and additionally carried within a portable organ transporter.
[0061] Fig. 7 shows an external view of an embodiment of a transporter 1900 of the invention. The transporter 1900 of Fig. 7 has a stable base to facilitate an upright position and handles 1910 for carrying transporter 1900. Transporter 1900 may also be fitted with a shoulder strap and/or wheels to assist in carrying transporter 1900. A control panel 1920 is preferably also provided. Control panel 1920 may display characteristics, such as, but not limited to, infusion pressure, attachment of the tube frame, power on/off, error or fault conditions, flow rate, flow resistance, infusion temperature, bath temperature, pumping time, battery charge, temperature profile (maximums and minimums), cover open or closed, history log or graph, and additional status details and messages, some or all of which are preferably further transmittable to a remote location for data storage and/or analysis.
Flow and pressure sensors or transducers in transporter 1900 may be provided to calculate various organ characteristics including pump pressure and vascular resistance of an organ, which can be stored in computer memory to allow for analysis of, for example, vascular resistance history, as well as to detect faults in the apparatus, such as elevated pressure.
[0062] Transporter 1900 preferably has latches 1930 that require positive user action to open, thus avoiding the possibility that transporter 1900 inadvertently opens during transport. Latches 1930 hold top 1940 in place on transporter 1900 in Fig. 7.
Top 1940 or a portion thereof may be constructed with an optically transparent material to provide for viewing of the cassette and organ perfusion status. Transporter 1900 may be configured with a cover open detector that monitors and displays whether the cover is open or closed.
Transporter 1900 may be configured with an insulating exterior of various thicknesses to allow the user to configure or select transporter 1900 for varying extents and distances of transport. In embodiments, compartment 1950 may be provided to hold patient and organ data such as charts, testing supplies, additional batteries, hand-held computing devices and/or configured with means for displaying a UNOS label and/or identification and return shipping information.
100631 Fig. 8 shows a cross-section view of a transporter 1900. Transporter contains cassette 65 and pump 2010. Cassette 65 may preferably be placed into or taken out of transporter 1900 without disconnecting tubeset 400 from cassette 65, thus maintaining sterility of the organ. In embodiments, sensors in transporter 1900 can detect the presence of cassette 65 in transporter 1900, and depending on the sensor, can read the organ identity from a barcode or radio frequency or other "smart" tag that may be attached or integral to cassette 65. This can allow for automated identification and tracking of the organ and helps monitor and control the chain of custody. A global positioning system may be added to transporter 1900 and/or cassette 65 to facilitate tracking of the organ. Transporter 1900 may be interfaceable to a computer network by hardwire connection to a local area network or by wireless communication while in transit. This interface may allow data such as perfusion parameters, vascular resistance, and organ identification and transporter and cassette location to be tracked and displayed in real-time or captured for future analysis.
[0064] Transporter 1900 also preferably contains a filter 2020 to remove sediment and other particulate matter, preferably ranging in size from 0.05 to 15 microns in diameter or larger, from the perfusate to prevent clogging of the apparatus or the organ.
Transporter 1900 preferably also contains batteries 2030, which may be located at the bottom of transporter 1900 or beneath pump 2010 or at any other location but preferably one that provides easy access to change batteries 2030. Batteries 2030 may be rechargeable outside of transporter 1900 or while within transporter 1900 and/or are preferably hot-swappable one at a time.
Batteries 2030 are preferably rechargeable rapidly and without full discharge.
Transporter 1900 may also provide an additional storage space 2040, for example, at the bottom of transporter 1900, for power cords, batteries and other accessories.
Transporter 1900 may also include a power port for a DC hookup, e.g., to a vehicle such as an automobile or airplane, and/or for an AC hookup.
[0065] As shown in Fig. 8, the cassette wall CW is preferably configured to mate with a corresponding configuration of inner transporter wall TW to maximize contact, and thus heat transfer, there between as discussed in more detail below.
[0066] Fig. 9 shows an alternative cross-section of transporter 1900. In Fig.
9, the transporter 1900 may have an outer enclosure 2310 which may, for example, be constructed of metal, or preferably a plastic or synthetic resin that is sufficiently strong to withstand penetration and impact. Transporter 1900 contains insulation 2320, preferably a thermal insulation made of, for example, glass wool or expanded polystyrene.
Insulation 2320 may be various thicknesses ranging from 0.5 inches to 5 inches thick or more, preferably 1 to 3 inches, such as approximately 2 inches thick. Transporter 1900 may be cooled by coolant 2110, which may be, e.g., an ice and water bath or a cryogenic material. In embodiments using cryogenic materials, the design should be such that organ freezing is prevented. An ice and water mixture is preferably an initial mixture of approximately 1 to 1, however, in embodiments the ice and water bath may be frozen solid. Transporter 1900 can be configured to hold various amounts of coolant, preferably up to 10 to 12 liters. An ice and water bath is preferable because it is inexpensive and generally can not get cold enough to freeze the organ. Coolant 2110 preferably lasts for a minimum of 6 to 12 hours and more preferably lasts for a minimum of 30 to 50 hours without changing coolant 2110. The level of coolant 2110 may, for example, be viewed through a transparent region of transporter 1900 or be automatically detected and monitored by a sensor. Coolant 2110 can preferably be replaced without stopping perfusion or removing cassette 65 from transporter 1900. Coolant 2110 is preferably maintained in a watertight compartment 2115 of transporter 1900. For example, an inner transporter wall TW as shown in Fig. 8 can be interposed between the coolant 2110 and cassette wall (CW) in the apparatus of Fig. 9. Compartment preferably prevents the loss of coolant 2110 in the event transporter 1900 is tipped or inverted. Heat is conducted from the walls of the perfusate reservoir/cassette 65 into coolant 2110 enabling control within the desired temperature range. Coolant 2110 is a failsafe cooling mechanism because transporter 1900 automatically reverts to cold storage in the case of power loss or electrical or computer malfunction. Transporter 1900 may also be configured with a heater to raise the temperature of the perfusate.
[0067] Transporter 1900 may be powered by batteries or by electric power provided through plug 2330. An electronics module 2335 may also be provided in transporter 1900.
Electronics module 2335 may be cooled by vented air convection 2370, and may further be cooled by a fan. Preferably, electronic module 2335 is positioned separate from the perfusion tubes to prevent the perfusate from wetting electronics module 2335 and to avoid adding extraneous heat from electronics module 2335 to the perfusate. Transporter 1900 preferably has a pump 2010 that provides pressure to perfusate tubing 2360 (e.g. of tube set 400) to deliver perfusate 2340 to organ 2350. Transporter 1900 may be used to perfuse various organs such as a kidney, heart, liver, pancreas, small intestine and lung.
Transporter 1900 and cassette 65 may accommodate various amounts to perfusate 2340, for example up to 3 to liters. Preferably, approximately 1 liter of a hypothermic perfusate 2340 is used to perfuse organ 2350.
[0068] Cassette 65 and transporter 1900 are preferably constructed to fit or mate such that efficient heat transfer is enabled. Preferably, the transporter 1900 contains a compaitinent 2115 for receiving the cassette. The transporter 1900 preferably relies on conduction to move heat from the cassette 65 to coolant 2110 contained in compartment 2115. This movement of heat allows the transporter 1900 to maintain a desired temperature of the perfusion solution. The geometric elements of cassette 65 and transporter 1900 are preferably constructed such that when cassette 65 is placed within transporter 1900, the contact area between cassette 65 and transporter 1900 is as large as possible and they are secured for transport.
[0069] Fig. 10 shows an example of this geometry between cassette 65 and compartment 2115 containing coolant 2110. The interface geometry between cassette 65 and compartment 2115 is preferably designed so that cassette 65 will wedge into a cavity created by compartment 2115. Accordingly, the angles of the side walls are substantially equal and thus, all side walls of cassette 65 make contact with the side walls of compartment 2115 regardless of the shape of the cassette sides or compartment sides, such as flat or curved.
Having the included angles substantially equal allows the surfaces of cassette 65 and the compartment 2115 to make contact even when influenced by the thermal expansion and contraction of the walls and mechanical tolerances.
[0070] The height of cassette 65 above compaitnient 2115 is determined by the mating surfaces of cassette 65 and compartment 2115. As shown in Fig. 10 the bottom of the cassette does not have to rest on the bottom of compartment 2115, but can rest at the bottom in embodiments in which the shape of cassette 65 and compartment 2115 allow it. It should be appreciated that the shape of cassette 65 and compartment 2115 in these embodiments can be any shape, such as for example a truncated cone, that allows for maximum contact and therefore maximum heat transfer between them.
[0071] As discussed above, heat is conducted from the walls of the perfusate reservoir/cassette 65 into coolant 2110 of compartment 2115 enabling control within the desired temperature range. Coolant 2110 can provide a failsafe cooling mechanism because transporter 1900 automatically reverts to cold storage in the case of power loss or electrical or computer malfunction. Transporter 1900 may also be configured with a heater to raise the temperature of the perfusate.

[0072] Figure 11 shows a tube frame 200 of embodiments of the invention, which may be used for holding tube set 400 discussed with respect to Fig. 6A. Tube frame 200 is preferably formed of a material that is light but durable, such as for example plastic, so that tube frame 200 is highly portable. The tube frame 200 is designed to hold the tubing of the tube set 400 in desired positions. In Fig. 11, tube frame 200 is shown holding the tubes of tube set 400 of Fig. 6A. It should be appreciated that there may be any number of tubes that comprise tube set 400. Having the tubing in set positions allows for easier installation and connection with devices such as cassette 65 as shown in Fig. 12. The cassette 65 and tube frame 200 are then preferably mated with transporter 1900.
[0073] When tube frame 200 is mated with cassette 65, the tube set 400 is preferably already connected with the cassette 65. For example, tube 270 provides an inlet to a pump 2010 from the stored liquid at the bottom of cassette 65. The liquid travels through tube 290 and back out outlet 280 through a filter which may, for example, be located inside or outside, for example, below, cassette 65. After traveling through the filter, the liquid will travel to tube 240 and into the bubble trap 210. A sample port 295 may be provided with tube frame 200 to allow for drawing liquid out of or injecting liquid into the tube 240. Liquid travels into the bubble trap 210 in tube 240 and travels out of bubble trap 210 in tube 260, which carries the liquid into the cassette, for example, to infuse and/or wash the organ. Tube 250 will carry liquid or gas leaving the bubble trap 210 into cassette 65 bypassing infusion of, but optionally washing, the organ.
[0074] It should be appreciated that tube frame 200 can hold other devices in addition to tubes. For example, tube frame 200 can hold a bubble trap device 210 and a pressure sensor 220 used to control pump 2010. It should also be appreciated that tube frame 200 and tube set 400 can be connected to a variety of devices such as the organ perfusion device 1 or an organ diagnostic device, as well as a cassette and/or transporter.
[0075] In various exemplary embodiments, tube frame 200 is preferably attachable to a portion of the transporter 1900. The tube frame 200 may be connected to transporter 1900, and other devices, by way of snaps 230 or other structure, for example clips or latches, that will securely hold the tube frame to the device. Sensors, for example mechanical or electrical sensors, in transporter 1900, or other devices, can be provided to detect the presence of tube frame 200 in transporter 1900. If the tube frame 200 is not properly attached to the transporter, the sensors may be configured to send an appropriate alert message to control panel 1920 for notifying the user of a problem. If no action is taken to properly attach tube frame 200 in a given amount of time automatically set or programmed by the user, transporter 1900 can be programmed to prevent the beginning of perfusion_ It should be appreciated that if perfusion has begun and tube frame 200 is not appropriately set, the transporter Can be programmed to stop perfusion.
(00761 Another valuable feature of the tube frame is that makes a stationary surface for the tube 250, and tube 260. These tubes are used to route perfusion solution either directly to the organ or, bypassing the organ, into the reservoir. It is desirable to have tube 250 and tube 260 located in a relatively fixed position so that the routing may be done by pinching the tubing so that no liquid can pass. The tubes may, for example, be pinched by a solenoid (not shown) located on transporter 1900 that drives a blade that pinches tube 250 and/or tube 260 against the tube frame 200.
100771 While the invention has been described in conjunction with specific embodiments thereof it is evident that many alternatives, modifications and variations may be apparent. to those skilled in the art. Accordingly, the embodiments of the invention as set forth herein are intended to be illustrative, not limiting. Various changes may be made without departing from the scope of the invention.
=

Claims (65)

1. Apparatus for holding an organ or a tissue for at least one of perfusion, storage, diagnosis and transport of the organ or tissue, comprising:
an organ or tissue transporter being configured to perfuse an organ or a tissue; a portable housing having one or more heat transfer surfaces;
a compartment within the transporter having one or more heat transfer surfaces arranged on an outer surface of the compartment to transfer heat between a cooling source contained within the compartment and at least a part of one of the one or more heat transfer surfaces of the portable housing, the compartment being liquid-tight and preventing contact between a liquid contained within the compartment and the portable housing;
and an organ or tissue supporting surface configured to support the organ or tissue within said portable housing, wherein the portable housing is configured to be received by the transporter and wherein at least a portion of at least one of the one or more heat transfer surfaces of the compartment is in contact with at least a portion of at least one of the one or more heat transfer surfaces of the portable housing to allow effective heat transfer to or from the contents of the portable housing.
2. The apparatus of claim 1, wherein the cooling source is a cooling fluid.
3. The apparatus of claim 2, wherein the cooling fluid is a cryogenic fluid.
4. The apparatus of claim 3, wherein the cryogenic fluid is water or water provided as ice.
5. The apparatus of claim 4, wherein the cryogenic fluid is a combination of water and ice.
6. The apparatus of claim 1, wherein the supporting surface is configured to support the organ or tissue while allowing effluent medical fluid to pass through said organ or tissue.
7. The apparatus of claim 1, wherein the outer surface of compartment and the portable housing have substantially complementary configurations.
8. The apparatus of claim 7, wherein a majority of at least one of the one or more heat transfer surfaces of the compartment is in contact with a majority of at least one of the one or more heat transfer surfaces of the portable housing.
9. The apparatus of claim 7, wherein substantially all of at least one of the one or more heat transfer surfaces of the compartment is in contact with substantially all of at least one of the one or more heat transfer surfaces of the portable housing.
10. The apparatus of claim 7, wherein the portable housing is configured to mate with the compartment.
11. The apparatus of claim 7, wherein each of the compartment and the portable housing have multiple heat transfer surfaces forming the outer surface of compartment and the portable housing, wherein the multiple heat transfer surfaces of each of the compartment and the portable housing are arranged such that a side cross-sectional view forms a trapezoid.
12. The apparatus of claim 7, wherein the outer surface of the compartment and the portable housing have complementary configurations forming a truncated cone.
13. The apparatus of claim 7, wherein the one or more heat transfer surfaces of the compartment and the one or more heat transfer surfaces of the portable housing are planar surfaces.
14. The apparatus of claim 7, wherein the one or more heat transfer surfaces of the compartment and the one or more heat transfer surfaces of the portable housing are non-planar surfaces.
15. The apparatus of claim 1, wherein the portable housing includes openings configured to allow tubing to pass through said openings and be connected to the organ.
16. The apparatus of claim 15, wherein the tubing can pass through the openings when the portable housing is mated with the compartment.
17. The apparatus of claim 15, wherein the tubing is configured to be mated with the portable housing, when the portable housing is removed from the compartment.
18. The apparatus of claim 1, wherein the transporter includes a heater.
19. The apparatus of claim 1, wherein the portable housing includes a handle that is accessible when the portable housing is mated with the compartment.
20. The apparatus of claim 1, wherein a bottom portion of the portable housing is liquid-tight and configured to collect medical fluid that has passed through a perfused organ to form an organ bath.
21. The apparatus of claim 1, wherein the transporter includes at least one sensor configured to detect a presence of the portable housing within the compartment.
22. A method of transporting an organ or a tissue, comprising:
placing the organ or tissue in a portable housing, the portable housing having one or more heat transfer surfaces;
placing the portable housing containing the organ or tissue in a compartment of a transporter configured to receive the portable housing and configured to perfuse the organ or tissue, the compartment having one or more heat transfer surfaces arranged on an outer surface of the compartment, the compartment being liquid-tight and preventing contact between a liquid contained within the compartment and the portable housing, wherein at least a portion of at least one of the one or more heat transfer surfaces of the compartment is in contact with at least a portion of at least one of the one or more heat transfer surfaces of the portable housing;

transferring heat between a cooling source contained within the compartment, via at least a part of one of the one or more heat transfer surfaces of the compartment, to or from the contents of the portable housing; and transporting the organ in said portable housing in said transporter.
23. The method of claim 22, further comprising placing the portable housing containing the organ in an organ or tissue diagnostic device configured to receive the portable housing; and diagnosing the organ or tissue without removal of the organ from the portable housing.
24. The method of claim 22, further comprising placing the portable housing containing the organ or tissue in a perfusion device configured to receive the portable housing; and perfusing the organ or tissue without removal of the organ from the portable housing.
25. The method of claim 22, further comprising connecting tubing to the organ.
26. The method of claim 22, wherein the cooling source is a cooling fluid.
27. The method of claim 26, wherein the cooling fluid is a cryogenic fluid.
28. The method of claim 27, wherein the cryogenic fluid is water or water provided as ice.
29. The method of claim 28, wherein the cryogenic fluid is a combination of water and ice.
30. The method of claim 26, wherein a bottom portion of the housing is liquid-tight and configured to collect medical fluid that has passed through a perfused organ to form an organ bath.
31. The method of claim 30, further comprising cooling the medical fluid with the cooling fluid.
32. The method of claim 22, wherein the outer surface of the compartment and the portable housing have substantially complementary configurations.
33. The method of claim 32, wherein each of the compartment and the portable housing have multiple heat transfer surfaces forming the outer surface of the compartment and the portable housing, wherein the multiple heat transfer surfaces of each of the compartment and the portable housing are arranged such that a side cross-sectional view forms a trapezoid.
34. The method of claim 32, wherein the outer surface of the compartment and the portable housing have complementary configurations forming a truncated cone.
35. The method of claim 22, wherein the portable housing includes tubing and connection devices to allow connection of an organ in the portable housing to tubing of at least one of the transporter, the organ diagnosis device and the organ perfusion device.
36. The method of claim 22, wherein the portable housing includes a handle and the handle is accessible when the portable housing is mated with the compartment.
37. The method of claim 22, wherein the one or more heat transfer surfaces of the compartment and the one or more heat transfer surfaces of the portable housing are planar surfaces.
38. The method of claim 22, wherein the one or more heat transfer surfaces of the compartment and the one or more heat transfer surfaces of the portable housing are non-planar surfaces.
39. The method of claim 22, wherein the portable housing includes openings configured to allow tubing to pass through said openings and be connected to the organ.
40. The method of claim 39, wherein the tubing can pass through the openings when the portable housing is mated with the compartment.
41. The method of claim 22, wherein the transporter includes a heater to heat the cooling source.
42. The method of claim 41, further comprising transferring heat from the cooling source to a medical fluid.
43. The method of claim 22, wherein the transporter includes at least one sensor configured to detect a presence of the portable housing within the compartment.
44. A system for holding an organ or tissue for transport of the organ or tissue, comprising:
an organ or tissue transporter;
a portable housing having one or more heat transfer surfaces;
a compartment within the transporter having one or more heat transfer surfaces arranged on an outer surface of the compartment to transfer heat between a cooling source contained within the compartment and at least a part of one of the one or more heat transfer surfaces of the portable housing, the compartment being liquid-tight and preventing contact between a liquid contained within the compartment and the portable housing;
and an organ or tissue supporting surface configured to support an organ or tissue within said portable housing;
wherein the portable housing is configured to be received by the transporter and wherein at least a portion of at least one of the one or more heat transfer surfaces of the compartment is in contact with at least a portion of at least one of the one or more heat transfer surfaces of the portable housing to allow effective heat transfer to or from the contents of the portable housing, wherein the portable housing includes openings configured to allow tubing to pass through said openings and be connected to the organ, the tubing being configured to pass through the openings when the portable housing is mated with the compartment and being configured to be mated with the portable housing when the portable housing is removed from the compartment, and wherein the portable housing includes a handle that is accessible when the portable housing is mated with the compartment.
45. The system of claim 44, wherein the cooling source is a cooling fluid.
46. The system of claim 45, wherein the cooling fluid is a cryogenic fluid.
47. The system of claim 46, wherein the cryogenic fluid is water or water provided as ice.
48. The system of claims 47, wherein the cryogenic fluid is a combination of water and ice.
49. The system of claim 44, wherein the supporting surface is configured to support the organ or tissue while allowing effluent medical fluid to pass through said organ or tissue.
50. The system of claim 44, wherein the outer surface of the compartment and the portable housing have substantially complementary configurations.
51. The system of claim 50, wherein a majority of at least one of the one or more heat transfer surfaces of the compartment is in contact with a majority of at least one of the one or more heat transfer surfaces of the portable housing.
52. The system of claim 50, wherein substantially all of at least one of the one or more heat transfer surfaces of the compartment is in contact with substantially all of at least one of the one or more heat transfer surfaces of the portable housing.
53. The system of claim 50, wherein the portable housing is configured to mate with the outer surface of the compartment.
54. The system of claim 50, wherein each of the compartment and the portable housing have multiple heat transfer surfaces forming the outer surface of the compartment and the portable housing, wherein the multiple heat transfer surfaces of each of the compartment and the portable housing are arranged such that a side cross-sectional view forms a trapezoid.
55. The system of claim 50, wherein the outer surface of the compartment and the portable housing have complementary configurations forming a truncated cone.
56. The system of claim 50, wherein the one or more heat transfer surfaces of the compartment and the one or more heat transfer surfaces of the portable housing are planar surfaces.
57. The system of claim 50, wherein the one or more heat transfer surfaces of the compartment and the one or more heat transfer surfaces of the portable housing are non-planar surfaces.
58. The system of claim 44, wherein the transporter includes a heater.
59. The system of claim 44, wherein a bottom portion of the portable housing is liquid-tight and configured to collect medical fluid that has passed through a perfused organ to form an organ bath.
60. The system of claim 44, further comprising:
at least one of an organ perfusion device and an organ diagnostic device, wherein the portable housing is configured to be received by the at least one organ perfusion device and the organ diagnostic device and to allow at least one of perfusion and diagnosis of the organ without removal of the organ from the portable housing.
61. The system of claim 44, wherein the transporter includes at least one sensor configured to detect a presence of the portable housing within the compartment.
62. The system of claim 61, wherein the portable housing includes identification information, and wherein the sensor identifies the portable housing by reading the identification information.
63. The system of claim 44, wherein the transporter is configured to perfuse the organ or tissue.
64. Apparatus for holding an organ or a tissue for at least one of perfusion, storage, diagnosis and transport of the organ or tissue, comprising:
an organ or tissue transporter; a portable housing having one or more heat transfer surfaces;
a compartment within the transporter having one or more heat transfer surfaces arranged on an outer surface of the compartment to transfer heat between a cooling source contained within the compartment and at least a part of one of the one or more heat transfer surfaces of the portable housing, the compartment being liquid-tight and preventing contact between a liquid contained within the compartment and the portable housing;
and an organ or tissue supporting surface configured to support the organ or tissue within said portable housing, wherein the portable housing is configured to be received by the transporter, wherein at least a portion of at least one of the one or more heat transfer surfaces of the compartment is in contact with at least a portion of at least one of the one or more heat transfer surfaces of the portable housing to allow effective heat transfer to or from the contents of the portable housing, wherein the portable housing includes openings configured to allow tubing to pass through said openings and be connected to the organ or tissue, and wherein the tubing can pass through the openings when the portable housing is mated with the compartment.
65. A method of transporting an organ or a tissue, comprising:
placing the organ or tissue in a portable housing, the portable housing having one or more heat transfer surfaces;
placing the portable housing containing the organ or tissue in a compartment of a transporter configured to receive the portable housing, the compartment having one or more heat transfer surfaces arranged on an outer surface of the compartment, the compartment being liquid-tight and preventing contact between a liquid contained within the compartment and the portable housing, wherein at least a portion of at least one of the one or more heat transfer surfaces of the compartment is in contact with at least a portion of at least one of the one or more heat transfer surfaces of the portable housing;
transferring heat between a cooling source contained within the compartment, via at least a part of one of the one or more heat transfer surfaces of the compartment, to or from the contents of the portable housing; and transporting the organ in said portable housing in said transporter, wherein the portable housing includes openings configured to allow tubing to pass through said openings and be connected to the organ, and wherein the tubing can pass through the openings when the portable housing is mated with the compartment.
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Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8409846B2 (en) 1997-09-23 2013-04-02 The United States Of America As Represented By The Department Of Veteran Affairs Compositions, methods and devices for maintaining an organ
US6977140B1 (en) 1998-09-29 2005-12-20 Organ Recovery Systems, Inc. Method for maintaining and/or restoring viability of organs
US6673594B1 (en) * 1998-09-29 2004-01-06 Organ Recovery Systems Apparatus and method for maintaining and/or restoring viability of organs
EP1259428B1 (en) * 2000-01-20 2008-03-12 Bioaccess, Inc. A method and apparatus for introducing a non-sterile component into a sterile device
US6677150B2 (en) * 2001-09-14 2004-01-13 Organ Transport Systems, Inc. Organ preservation apparatus and methods
US20040170950A1 (en) * 2002-09-12 2004-09-02 Prien Samuel D. Organ preservation apparatus and methods
US7998725B2 (en) * 2003-04-04 2011-08-16 Organ Recovery Systems Method and apparatus for holding a plurality of tubes connectible to an organ or tissue container
US20050153271A1 (en) * 2004-01-13 2005-07-14 Wenrich Marshall S. Organ preservation apparatus and methods
US7504201B2 (en) * 2004-04-05 2009-03-17 Organ Recovery Systems Method for perfusing an organ and for isolating cells from the organ
ES2348736T3 (en) 2004-10-07 2010-12-13 Transmedics, Inc. SYSTEMS AND METHODS FOR EX-LIVE ORGAN CARE.
US8304181B2 (en) 2004-10-07 2012-11-06 Transmedics, Inc. Method for ex-vivo organ care and for using lactate as an indication of donor organ status
US9301519B2 (en) 2004-10-07 2016-04-05 Transmedics, Inc. Systems and methods for ex-vivo organ care
US9078428B2 (en) 2005-06-28 2015-07-14 Transmedics, Inc. Systems, methods, compositions and solutions for perfusing an organ
US8147408B2 (en) * 2005-08-31 2012-04-03 Sonosite, Inc. Medical device guide locator
DK3178319T3 (en) 2006-04-19 2020-02-03 Transmedics Inc EX VIVO ORGAN CARE SYSTEMS
US20080145919A1 (en) * 2006-12-18 2008-06-19 Franklin Thomas D Portable organ and tissue preservation apparatus, kit and methods
CA2679827C (en) 2007-03-01 2014-11-25 Lifeline Scientific, Inc. Perfusion regulation
US9457179B2 (en) 2007-03-20 2016-10-04 Transmedics, Inc. Systems for monitoring and applying electrical currents in an organ perfusion system
ES2538782T3 (en) * 2007-09-28 2015-06-24 Organ Assist B.V. Container system for organ transport
US9814230B2 (en) 2008-01-31 2017-11-14 Transmedics, Inc. Systems and methods for ex vivo lung care
EP2098809B1 (en) * 2008-03-06 2011-02-16 Eutecma GmbH Cooling element with excessive cooling protection
CA2722615C (en) * 2008-04-22 2018-01-16 The Board Of Regents Of The University Of Texas System Fluidics-based pulsatile perfusion organ preservation device
CA2723706C (en) * 2008-05-06 2020-10-20 Vivoline Medical Ab Apparatus for housing an organ during evaluation and preservation
US8790874B2 (en) * 2008-06-17 2014-07-29 Axel Guenther Device for investigation of a flow conduit
US9284523B2 (en) * 2008-10-27 2016-03-15 Terumo Bct, Inc. Premounted fluid conveyance assembly for cell expansion system and method of use associated therewith
US20130323708A1 (en) 2009-07-01 2013-12-05 Massachusetts Institute Of Technology Isolated adult cells, artificial organs, rehabilitated organs, research tools, organ encasements, organ perfusion systems, and methods for preparing and utilizing the same
EP2480069A1 (en) * 2009-09-25 2012-08-01 Board of Regents, The University of Texas System Fluidics based pulsatile perfusion preservation device and method
WO2011037512A1 (en) 2009-09-25 2011-03-31 Vivoline Medical Ab Container and supporting structure for housing an organ
WO2011126650A1 (en) * 2010-04-09 2011-10-13 Caridianbct, Inc. Air removal chamber for a cell expansion system and method of use associated therewith
US9677042B2 (en) 2010-10-08 2017-06-13 Terumo Bct, Inc. Customizable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US9119392B2 (en) * 2010-11-09 2015-09-01 Biomedinnovations, Llc Articulating organ support
WO2012078968A2 (en) 2010-12-10 2012-06-14 Lifeline Scientific, Inc. Machine perfusion with complement inhibitors
US20120178076A1 (en) * 2011-01-07 2012-07-12 Hiroyuki Fujita Magnetic resonance and non-magnetic resonance analysis
US9253976B2 (en) 2011-03-15 2016-02-09 Paragonix Technologies, Inc. Methods and devices for preserving tissues
US9426979B2 (en) 2011-03-15 2016-08-30 Paragonix Technologies, Inc. Apparatus for oxygenation and perfusion of tissue for organ preservation
US8828710B2 (en) 2011-03-15 2014-09-09 Paragonix Technologies, Inc. System for hypothermic transport of samples
US11178866B2 (en) 2011-03-15 2021-11-23 Paragonix Technologies, Inc. System for hypothermic transport of samples
US8835158B2 (en) 2011-03-15 2014-09-16 Paragonix Technologics, Inc. Apparatus for oxygenation and perfusion of tissue for organ preservation
US9867368B2 (en) 2011-03-15 2018-01-16 Paragonix Technologies, Inc. System for hypothermic transport of samples
US20130011823A1 (en) 2011-04-14 2013-01-10 Hassanein Waleed H Organ care solution for ex-vivo machine perfusion of donor lungs
US8828034B2 (en) 2011-04-29 2014-09-09 Lifeline Scientific, Inc. Cannula
US9022978B2 (en) 2011-04-29 2015-05-05 Lifeline Scientific, Inc. Universal sealring cannula
US9642625B2 (en) 2011-04-29 2017-05-09 Lifeline Scientific, Inc. Cannula for a donor organ with or without an aortic cuff or patch
US9955682B2 (en) * 2011-04-29 2018-05-01 Lifeline Scientific, Inc. Portable organ transportation system
US20150253232A1 (en) * 2011-05-02 2015-09-10 The Governing Council Of The University Of Toronto Device for investigation of a flow conduit
EP2750499B1 (en) 2011-09-02 2016-07-20 Organ Assist B.V. Apparatus, system and method for conditioning and preserving an organ from a donor
BR112014011358B1 (en) * 2011-11-10 2021-04-06 Organox Limited DISPOSABLE SET OF COMPONENTS FOR AN ORGAN PERFUSION SYSTEM, SYSTEM AND SUPPORT PANEL
CA2855337C (en) 2011-11-10 2020-08-18 Organox Limited Organ perfusion systems
GB201119420D0 (en) 2011-11-10 2011-12-21 Organox Ltd Oxygen supply for organ perfusion systems
US10258032B2 (en) 2012-01-11 2019-04-16 Lifeline Scientific, Inc. Transportation bag for use with an organ transporter
US8894647B2 (en) 2012-01-13 2014-11-25 Covidien Lp System and method for performing surgical procedures with a reusable instrument module
US9241757B2 (en) 2012-01-13 2016-01-26 Covidien Lp System and method for performing surgical procedures with a reusable instrument module
US9636091B2 (en) 2012-01-13 2017-05-02 Covidien Lp Hand-held electromechanical surgical system
ES2946695T3 (en) 2012-01-17 2023-07-24 Tevosol Inc Apparatus for testing, evaluation, and maintenance of hearts removed for transplantation
US9115936B2 (en) * 2012-02-15 2015-08-25 Wave Power Technology, Inc. Cooling apparatus and method using a vacuum pump
US10602740B2 (en) * 2012-07-10 2020-03-31 Lifeline Scientific, Inc. Organ perfusion apparatus with downstream flow control
US9402389B2 (en) 2012-07-10 2016-08-02 Lifeline Scientific, Inc. Organ transport apparatus with sample compartments
US11439143B2 (en) 2012-07-10 2022-09-13 Lifeline Scientific, Inc. Temperature sensing in organ preservation apparatus
US20140017666A1 (en) * 2012-07-10 2014-01-16 Lifeline Scientific, Inc. Filtration in organ perfusion apparatus
US9357767B2 (en) 2012-07-10 2016-06-07 Lifeline Scientific, Inc. Organ transporter
US9357766B2 (en) 2012-07-10 2016-06-07 Lifeline Scientific, Inc. Organ transport apparatus with a document compartment and a tamper evident seal
US9560846B2 (en) 2012-08-10 2017-02-07 Paragonix Technologies, Inc. System for hypothermic transport of biological samples
US8785116B2 (en) 2012-08-10 2014-07-22 Paragonix Technologies, Inc. Methods for evaluating the suitability of an organ for transplant
WO2014059316A1 (en) 2012-10-12 2014-04-17 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Compositions and methods for organ preservation
US10918102B2 (en) 2014-03-13 2021-02-16 The General Hospital Corporation Devices and methods to improve and assess viability of human livers
US10327442B2 (en) 2014-03-26 2019-06-25 Tevosol, Inc. Apparatus for maintenance of harvested hearts for transplanting
EP3151663B1 (en) 2014-06-02 2020-09-23 Transmedics, Inc. Ex vivo organ care system
USD765874S1 (en) 2014-10-10 2016-09-06 Paragonix Technologies, Inc. Transporter for a tissue transport system
EP3229588A4 (en) * 2014-12-12 2019-03-13 Freed, Darren Apparatus and method for organ perfusion
EP3824731A1 (en) 2015-09-09 2021-05-26 Transmedics, Inc. Aortic cannula for ex vivo organ care system
WO2017070639A1 (en) * 2015-10-23 2017-04-27 Tatom Patrick Alan Device for cooling substances
US10091986B2 (en) 2016-05-09 2018-10-09 Xor-Labs Toronto Inc. Organ perfusion device and method
WO2018024876A1 (en) * 2016-08-05 2018-02-08 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods and compositions for the preservation of organs
US11299705B2 (en) * 2016-11-07 2022-04-12 Deka Products Limited Partnership System and method for creating tissue
US10602741B2 (en) 2016-11-28 2020-03-31 Universidad Del Valle Organ-chimerization maintenance apparatus and method of use
CA3061643A1 (en) 2017-04-28 2018-11-01 Lifeline Scientific, Inc. Organ transporter with supplemental oxygenation system
US11285471B2 (en) * 2017-05-30 2022-03-29 Nextern Innovation, Llc Portable controlled micro-environment device for modular bio-processing
WO2018226993A1 (en) 2017-06-07 2018-12-13 Paragonix Technologies, Inc. Apparatus for tissue transport and preservation
CN107897169B (en) * 2017-11-03 2023-09-15 四川大学华西医院 Isolated organ oxygenation perfusion device
WO2021041181A1 (en) * 2019-08-23 2021-03-04 Paragonix Technologies, Inc. Pressurized system for tissue transport and preservation
US11632951B2 (en) 2020-01-31 2023-04-25 Paragonix Technologies, Inc. Apparatus for tissue transport and preservation
EP3977854A1 (en) * 2020-10-02 2022-04-06 Aferetica S.r.l. Thermoregulation system for an ex vivo organ perfusion system and corresponding ex vivo organ perfusion system
US20230092486A1 (en) 2021-09-23 2023-03-23 Lifeline Scientific, Inc. Low fluid level detection device

Family Cites Families (123)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1682344A (en) 1928-08-28 lesieur
US1369367A (en) * 1920-01-05 1921-02-22 Henry M Whitney Cooling-container
US1916658A (en) * 1931-10-09 1933-07-04 Louis R Davidson Pneumothorax apparatus
US3406531A (en) * 1964-08-25 1968-10-22 Emil S. Swenson Apparatus for maintaining organs in a completely viable state
US3545221A (en) 1967-05-22 1970-12-08 Swenko Research & Dev Inc Apparatus for maintaining organs in vitro in a completely viable state
FR1577356A (en) 1968-04-04 1969-08-08
US3545225A (en) * 1968-08-16 1970-12-08 Swenko Research & Dev Inc Apparatus for maintaining whole organs in a viable state
SE323475B (en) 1968-11-26 1970-05-04 Aga Ab
US3772153A (en) * 1969-04-02 1973-11-13 Air Liquide Apparatus for the preservation of animal or human organs in living condition
US3632473A (en) * 1969-04-21 1972-01-04 Univ California Method and apparatus for preserving human organs extracorporeally
BE755423A (en) 1969-10-06 1971-02-01 Baxter Laboratories Inc PROCESS AND APPARATUS FOR ORGAN PRESERVATION
US3892628A (en) * 1969-10-06 1975-07-01 Baxter Laboratories Inc Preservation of organs
US3660241A (en) * 1970-01-12 1972-05-02 Baxter Laboratories Inc Container for organ perfusion or the like
US3639084A (en) 1970-04-06 1972-02-01 Baxter Laboratories Inc Mechanism for control pulsatile fluid flow
US3810367A (en) * 1970-07-16 1974-05-14 W Peterson Container for cooling, storage, and shipping of human organ for transplant
US3712583A (en) * 1971-01-25 1973-01-23 Ametek Inc Valved for lines carrying extreme temperature fluids
US3753865A (en) 1971-03-12 1973-08-21 Univ California Method and apparatus for preserving human organs extracorporeally
US3713302A (en) * 1971-05-03 1973-01-30 Lek Trol Inc Personal insulin cooler
DE2241698C2 (en) * 1971-09-02 1982-08-26 Roland Dr.med. Zürich Doerig Process for organ preservation and device for carrying out this process
US3935065A (en) * 1971-09-02 1976-01-27 Roland Karl Doerig Procedure for conservation of living organs and apparatus for the execution of this procedure
US3881990A (en) 1971-11-24 1975-05-06 Waters Instr Inc Method of transporting and storing organs while retaining the organs in a viable condition
US3777507A (en) 1971-11-24 1973-12-11 Waters Instr Inc Renal preservation system
FR2169488A5 (en) * 1972-01-27 1973-09-07 Air Liquide
US3843455A (en) * 1972-09-13 1974-10-22 M Bier Apparatus and technique for preservation of isolated organs through perfusion
US3877843A (en) 1973-05-21 1975-04-15 Baxter Laboratories Inc Pulsatile pumping system
JPS5331209B2 (en) * 1973-10-05 1978-09-01
US3995444A (en) 1974-11-08 1976-12-07 American Hospital Supply Corporation Organ perfusion system
US4186565A (en) * 1978-05-19 1980-02-05 Henry Ford Hospital Perfusion system for organ preservation
US4231354A (en) 1978-07-14 1980-11-04 Howmedica, Incorporated Pulsatile blood pumping apparatus and method
US4243883A (en) 1979-01-19 1981-01-06 Midwest Cardiovascular Institute Foundation Blood hematocrit monitoring system
US4242883A (en) * 1979-04-02 1981-01-06 Henry Ford Hospital Liver preservation
US4393863A (en) * 1980-04-14 1983-07-19 Thomas Jefferson University Extravascular circulation of oxygenated synthetic nutrients to treat tissue hypoxic and ischemic disorders
US4657532A (en) * 1985-07-19 1987-04-14 Thomas Jefferson University Intra-peritoneal perfusion of oxygenated fluorocarbon
US4445500A (en) * 1982-03-03 1984-05-01 Thomas Jefferson University Stroke treatment utilizing extravascular circulation of oxygenated synthetic nutrients to treat tissue hypoxic and ischemic disorders
US4378797A (en) * 1980-04-14 1983-04-05 Thomas Jefferson University Extravascular circulation of oxygenated synthetic nutrients to treat tissue hypoxic and ischemic disorders
US5085630A (en) * 1980-04-14 1992-02-04 Thomas Jefferson University Oxygenated fluorocarbon nutrient solution
US4717548A (en) * 1980-06-09 1988-01-05 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Analytically controlled blood perfusion system
US4629686A (en) 1982-02-19 1986-12-16 Endotronics, Inc. Apparatus for delivering a controlled dosage of a chemical substance
US4451251A (en) * 1982-03-03 1984-05-29 Thomas Jefferson University Stroke treatment utilizing extravascular circulation of oxygenated synthetic nutrients to treat tissue hypoxic and ischemic disorders
CA1200507A (en) * 1982-06-04 1986-02-11 Nobuo Sakao Method of preserving organ and apparatus for preserving the same
US4460102A (en) * 1982-09-23 1984-07-17 Barringer Albert J Sealed container
US4473637A (en) 1982-11-10 1984-09-25 Guibert, Colman & Associates System for processing an organ preparatory to transplant
US4559298A (en) 1982-11-23 1985-12-17 American National Red Cross Cryopreservation of biological materials in a non-frozen or vitreous state
US4474016A (en) * 1983-03-04 1984-10-02 Baxter Travenol Laboratories, Inc. Sterile cooling system
US4618586A (en) 1983-04-08 1986-10-21 Endotronics, Inc. Apparatus for administering a controlled dosage of a chemical substance having an improved culture chamber
US4837390A (en) 1983-05-11 1989-06-06 Keyes Offshore, Inc. Hyperbaric organ preservation apparatus and method for preserving living organs
US4462215A (en) * 1983-05-31 1984-07-31 Hoxan Corporation Method of preserving organ and apparatus for preserving the same
US4471629A (en) 1983-05-31 1984-09-18 Mount Carmel Research And Education Corporation Method of freezing and transplant of kidneys and apparatus
US4801299A (en) 1983-06-10 1989-01-31 University Patents, Inc. Body implants of extracellular matrix and means and methods of making and using such implants
US4530816A (en) * 1983-06-15 1985-07-23 Hamilton Farm Method and device for cooling, preserving and safely transporting biological material
US4502295A (en) 1984-02-21 1985-03-05 Mount Carmel Research And Education Corporation Organ hypothermic storage unit
US4596250A (en) * 1984-11-14 1986-06-24 Genetic Laboratories, Inc. Moldable cooling/heating device with directional cooling/heating
FR2583386B1 (en) * 1985-06-12 1987-09-18 Sartec Service Ind VALVE FOR PACKAGING AND PACKAGING COMPRISING SUCH A VALVE
US4723974A (en) * 1985-07-26 1988-02-09 Ammerman Stephen W Transporting container for an amputated extremity
US4879283A (en) 1985-10-03 1989-11-07 Wisconsin Alumni Research Foundation Solution for the preservation of organs
US4666425A (en) 1985-12-17 1987-05-19 The Dis Corporation Device for perfusing an animal head
US4704029A (en) 1985-12-26 1987-11-03 Research Corporation Blood glucose monitor
GB8619437D0 (en) * 1986-08-08 1986-09-17 Bradley L Storage & refrigeration
US4745759A (en) 1986-12-23 1988-05-24 Bauer Dan O Kidney preservation machine
GB8711614D0 (en) 1987-05-16 1987-06-24 Medical Res Council Proteins
US5051352A (en) * 1987-10-07 1991-09-24 The Regents Of The University Of California Apparatus and method of preserving the viability of animal organs
JPH0626138B2 (en) * 1987-11-20 1994-04-06 昭和電工株式会社 Secondary battery
FR2628077B1 (en) 1988-03-07 1990-08-03 Guilhem Jacques CONTAINER FOR TRANSPORTING GRAFT
EP0337677A3 (en) * 1988-04-11 1990-04-04 Costar Corporation Vent cap
US5130230A (en) * 1988-05-02 1992-07-14 Cryomedical Sciences, Inc. Blood substitute
AU633280B2 (en) * 1988-10-26 1993-01-28 Michael Kaye A device for transportation of human organs used for transplantation
US5013303A (en) * 1988-11-03 1991-05-07 Yehuda Tamari Constant pressure infusion device
US4951482A (en) * 1988-12-21 1990-08-28 Gilbert Gary L Hypothermic organ transport apparatus
US5110721A (en) * 1989-02-10 1992-05-05 The Research Foundation Of State University Of New York Method for hypothermic organ protection during organ retrieval
US5326706A (en) * 1989-07-17 1994-07-05 Research Foundation Of State University Of New York Homeostatic organ preservation system
RU2079273C1 (en) * 1989-07-27 1997-05-20 И.Джост Леонора Transportable container of enclosed system for storing biological material
JPH0653160B2 (en) 1989-08-18 1994-07-20 呉羽化学工業株式会社 Beat generation method and device
US5200176A (en) * 1989-10-06 1993-04-06 Genentech, Inc. Method for protection of ischemic tissues using tumor nerosis factor
US5036097A (en) * 1989-10-17 1991-07-30 Oklahoma Medical Research Foundation Phenylbutyl nitrone compositions and methods for prevention of gastric ulceration
US5066578A (en) 1989-12-21 1991-11-19 The Regents Of The University Of California Long-term preservation of organs for transplantation
US5003787A (en) * 1990-01-18 1991-04-02 Savant Instruments Cell preservation system
US5145771A (en) 1990-04-12 1992-09-08 The University Of North Carolina At Chapel Hill Rinse solution for organs and tissues
US5047395A (en) 1990-07-17 1991-09-10 Nagase Co., Ltd. Reduction of oxyradical damage in biomedical applications
FR2667297B1 (en) * 1990-09-28 1994-05-27 Electrolux Sarl AIR-CONDITIONED MEDICAL CONTAINER.
US5395314A (en) 1990-10-10 1995-03-07 Life Resuscitation Technologies, Inc. Brain resuscitation and organ preservation device and method for performing the same
US5149321A (en) * 1990-10-10 1992-09-22 Klatz Ronald M Brain resuscitation device and method for performing the same
US5584804A (en) 1990-10-10 1996-12-17 Life Resuscitation Technologies, Inc. Brain resuscitation and organ preservation device and method for performing the same
US5827222A (en) 1990-10-10 1998-10-27 Life Resuscitation Technologies, Inc. Method of treating at least one of brain and associated nervous tissue injury
US5308320A (en) * 1990-12-28 1994-05-03 University Of Pittsburgh Of The Commonwealth System Of Higher Education Portable and modular cardiopulmonary bypass apparatus and associated aortic balloon catheter and associated method
US5157930A (en) * 1991-04-22 1992-10-27 Mcghee Samuel C Organ preservation apparatus
US5217860A (en) 1991-07-08 1993-06-08 The American National Red Cross Method for preserving organs for transplantation by vitrification
US5723282A (en) * 1991-07-08 1998-03-03 The American National Red Cross Method of preparing organs for vitrification
US5856081A (en) * 1991-07-08 1999-01-05 The American National Red Cross Computer controlled cryoprotectant perfusion apparatus
US5216032A (en) * 1991-09-30 1993-06-01 The University Of North Carolina At Chapel Hill Selective aortic arch perfusion using perfluorochemical and alpha adrenergic agonist to treat cardiac arrest
US5328821A (en) * 1991-12-12 1994-07-12 Robyn Fisher Cold and cryo-preservation methods for human tissue slices
US5338662A (en) 1992-09-21 1994-08-16 Bio-Preserve Medical Corporation Organ perfusion device
DE4301524A1 (en) * 1993-01-21 1994-07-28 Jostra Medizintechnik Medical unit or device for operating theaters, in particular heart-lung machine
JP3113886B2 (en) * 1993-03-03 2000-12-04 デカ、プロダクツ、リミテッド、パートナーシップ Peritoneal dialysis system using liquid dispensing and pump cassettes with built-in air isolation and removal
US5356771A (en) 1993-03-11 1994-10-18 Board Of Regents, The University Of Texas System Combined perfusion and oxygenation organ preservation apparatus
US5362622A (en) * 1993-03-11 1994-11-08 Board Of Regents, The University Of Texas System Combined perfusion and oxygenation apparatus
US5599659A (en) 1993-03-11 1997-02-04 Breonics, Inc. Preservation solution for ex vivo, warm preservation of tissues, explants,organs and vascular endothelial cells comprising retinal-derived fibroblast growth factor, cyclodextrin and chondroitin sulfate
AU6409594A (en) 1993-03-16 1994-10-11 Alliance Pharmaceutical Corporation Preservation solution and method for warm organ preservation
US5462416A (en) * 1993-12-22 1995-10-31 Baxter International Inc. Peristaltic pump tube cassette for blood processing systems
US5437633A (en) 1994-03-30 1995-08-01 The University Of North Carolina At Chapel Hill Selective aortic arch perfusion
US5643712A (en) 1994-05-20 1997-07-01 Brasile; Lauren Method for treating and rendering grafts nonthrombogenic and substantially nonimmunogenic using an extracellular matrix coating
ATE423464T1 (en) 1994-05-20 2009-03-15 Breonics Inc METHOD FOR MONITORING THE VIABILITY OF TRANSPLANTABLE ORGANS
US5823986A (en) * 1995-02-08 1998-10-20 Medtronic, Inc. Perfusion system
US5586438A (en) 1995-03-27 1996-12-24 Organ, Inc. Portable device for preserving organs by static storage or perfusion
US5681740A (en) * 1995-06-05 1997-10-28 Cytotherapeutics, Inc. Apparatus and method for storage and transporation of bioartificial organs
US5730720A (en) 1995-08-18 1998-03-24 Ip Scientific, Inc. Perfusion hyperthermia treatment system and method
US5712084A (en) 1995-09-08 1998-01-27 Research Corporation Technologies, Inc. Donor kidney viability test for improved preservation
US5712583A (en) * 1995-11-13 1998-01-27 International Business Machines Corporation Clock phase alignment using frequency comparison
JPH09167708A (en) * 1995-12-15 1997-06-24 Toko Inc Inverter transformer
US5843024A (en) 1996-05-17 1998-12-01 Breonics, Inc. Solution and process for resuscitation and preparation of ischemically damaged tissue
US5965433A (en) 1996-05-29 1999-10-12 Trans D.A.T.A. Service, Inc. Portable perfusion/oxygenation module having mechanically linked dual pumps and mechanically actuated flow control for pulsatile cycling of oxygenated perfusate
US5716378A (en) * 1996-06-06 1998-02-10 Medtronic, Inc. Heart preservation and transportation apparatus and method employing low rate cardiac pacing for improved removal of catabolites from the myocardium
US5879329A (en) * 1997-01-22 1999-03-09 Radiant Medical, Inc. Infusion systems and methods for introducing fluids into the body within a desired temperature range
US5928182A (en) 1997-07-02 1999-07-27 Johnson & Johnson Professional, Inc. Pediatric programmable hydrocephalus valve
US6953655B1 (en) * 1997-09-23 2005-10-11 The United States Of America As Represented By The Department Of Veterans Affairs Compositions, methods and devices for maintaining an organ
US6100082A (en) * 1997-09-23 2000-08-08 Hassanein; Waleed H. Perfusion apparatus and method including chemical compositions for maintaining an organ
US6673594B1 (en) 1998-09-29 2004-01-06 Organ Recovery Systems Apparatus and method for maintaining and/or restoring viability of organs
US6582953B2 (en) * 1999-04-14 2003-06-24 Breonics, Inc. Organ chamber for exsanguinous metabolic support system
AUPQ973800A0 (en) * 2000-08-28 2000-09-21 Automated Plastic Systems Pty Ltd Medical transport container
US20080027368A1 (en) * 2000-09-27 2008-01-31 Sorin Group Usa, Inc. Disposable cartridge for a blood perfusion system
AU2001294922A1 (en) * 2000-10-02 2002-04-15 Thomas F. Cannon Automated bioculture and bioculture experiments system
US6677150B2 (en) * 2001-09-14 2004-01-13 Organ Transport Systems, Inc. Organ preservation apparatus and methods
US20040029266A1 (en) * 2002-08-09 2004-02-12 Emilio Barbera-Guillem Cell and tissue culture device
US7998725B2 (en) * 2003-04-04 2011-08-16 Organ Recovery Systems Method and apparatus for holding a plurality of tubes connectible to an organ or tissue container

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