CN100384390C - Complex tissue of tissue-engineered bone and cartilage and external constructing method thereof - Google Patents
Complex tissue of tissue-engineered bone and cartilage and external constructing method thereof Download PDFInfo
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- CN100384390C CN100384390C CNB2005100019687A CN200510001968A CN100384390C CN 100384390 C CN100384390 C CN 100384390C CN B2005100019687 A CNB2005100019687 A CN B2005100019687A CN 200510001968 A CN200510001968 A CN 200510001968A CN 100384390 C CN100384390 C CN 100384390C
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Abstract
The present invention discloses a composite tissue of a tissue engineering bone and a cartilage, which comprises a tissue engineering bone and a tissue engineering cartilage, wherein the tissue engineering bone and the tissue engineering cartilage are embedded together through a groove and a bulge which are arranged in the position of a combination interface and are mutually in concave and convex relation. Biologic protein gel is sprayed and coated between two kinds of tissue interfaces. The present invention also discloses an in vitro construction method for the composite tissue. The interface structure of the composite tissue of a tissue engineering bone and a cartilage of the present invention has the advantages of complete and firm tissue structure, convenient assembly and easily obtained raw materials. The biologic protein gel also prevents seed cells in different tissues from freely moving in different tissues.
Description
Technical field
The present invention relates to tissue engineered bone and cartilage complex tissue that a kind of histoorgan defect repair is used, and the method for the external structure of this complex tissue.
Background technology
The serious damaged or handicapped effective measures of clinical treatment histoorgan are that histoorgan is transplanted at present, but this Therapeutic Method has many defectives, and is limited as: donor tissue organ origin, transplanted tissue's organ is produced immunological rejection and increases viral infection probability etc.The eighties in 20th century, along with the deep development of basic subjects such as cytobiology and Engineering Materials, scientist begins to probe boldly the new method of this world medicine difficult problem of reasonable solution.The notion of " organizational project " produces thus, and it is the principle and the method for application project and life sciences, and the substitute of preparation biologically active is to recover, to keep or improve the function of damaged tissues.The ultimate principle and the method for organizational project are: with the normal tissue cell of cultured and amplified in vitro as seed cell, be adsorbed on the good and biologic bracket material that can be absorbed by body of a kind of biocompatibility and form complex, the disease of cell-scaffold material composite implanting to human body tissue, organ is decreased the position, seed cell biomaterial form in gradually by the process of body degraded and absorbed new at form and function aspects and corresponding organ, organize corresponding to tissue, and reach the purpose of repairing wound and reconstruction function.
Adopt the learn a skill histoorgan of repair deficiency of organizational project, having graft materials wide material sources, graft can be in external shaping, avoid or reduced advantage such as sending out of immunological rejection and infectious disease.Adopt tissue engineering technique successfully to make up single engineering tissues such as skin, bone, cartilage, tendon, liver, cardiac muscle, bladder at present.
The structure of tissue engineered bone and tissue engineering bone/cartilage can be divided into construct in vitro and two kinds of forms of external structure.Construct in vitro is that seed cells such as osteoblast or chondrocyte is directly compound with unfashioned timbering material, adopts the mode of injection that complex is implanted then, the moulding in vivo filling defect area of complex, and then repair bone or cartilage defect.External structure is main building mode, and it is in the external respective holder material that the seed cell direct inoculation is being shaped, and through after the external preliminary cultivation, directly implants defect area by performing the operation, and then repairs bone and cartilage defect.Vitro tissue is cultivated and is comprised dynamic cultivation and static culture.
But, in the orthopaedics clinical treatment, often run into the patient and have the damaged of bone and cartilaginous tissue simultaneously, need finish the reparation of defective tissue simultaneously, therefore the tissue engineered bone of single component or tissue engineering bone/cartilage are transplanted and are subjected to greatly limiting.Carry out compound tense at the tissue engineered bone and the tissue engineering bone/cartilage that adopt single component, the interface processing of two kinds of tissues is a difficult point and key point, be mainly reflected in following several aspect: (1) is because cartilage is more flexible tissue, tissue regeneration ability extreme difference, and thickness is 2～4mm only, if be transplanted to intraarticular fixing unstable, the formation joint bodies very easily comes off.(2) if simultaneously when transplanting single tissue engineered bone and tissue engineering bone/cartilage, the interface is the plane, easily is shifted mutually between two kinds of tissues, causes repairing failure, at present this is not still had ideal fixation.(3) cell of bone and cartilaginous tissue has diverse function, if the single organization's engineering bone and the direct compound tense of tissue engineering bone/cartilage that make up, because timbering material is a mesh-like structure, seed cell in the different tissues can be by organizational interface's free migration between two kinds of tissues, thereby influence the repairing effect of tissue defect greatly, even cause repairing failure.
Summary of the invention
The objective of the invention is to provide a kind of tissue engineered bone and cartilage complex tissue, the tissue engineered bone of this complex tissue and cartilage are difficult for being shifted, and also can avoid transplanting seed cell in the back different tissues at the interface free migration.
The technical solution used in the present invention is: tissue engineered bone and cartilage complex tissue, comprise tissue engineered bone and tissue engineering bone/cartilage, tissue engineered bone and tissue engineering bone/cartilage by mutually combine at the interface each other the groove of concavo-convex relationship and projection be entrenched togather, between two kinds of organizational interfaces, be coated with the bioprotein gel.
The present invention also provides the method for a kind of tissue engineered bone and tissue engineering bone/cartilage complex tissue external structure, and this method comprises following process:
(1) external preparation is used for the timbering material of tissue construction: with mould preparation or purchase timbering material, carry out the interface pretreatment, the interface that bone tissue engineering scaffold and tissue engineering bone/cartilage timbering material are mutually combined becomes the groove and the bulge-structure of concavo-convex relationship each other;
(2) the external independent structure of tissue engineered bone and tissue engineering bone/cartilage: the skeletonization seed cell of external a large amount of amplifications with to become the cartilage seed cell compound with corresponding bone tissue engineering scaffold and tissue engineering bone/cartilage timbering material respectively, carried out static state or dynamically cultivates external then;
(3) with the combination interface sprayed biological protein gel of cultured tissue engineered bone and two kinds of tissues of tissue engineering bone/cartilage, utilize its groove and bulge-structure assembling of concavo-convex relationship each other to be entrenched togather then, form complex tissue.
The invention has the beneficial effects as follows: (1) tissue engineered bone and tissue engineering bone/cartilage combination interface are entrenched togather by concaveconvex structure, increased the fastness of complex tissue, cartilaginous tissue is fixed on the osseous tissue, solved the fixation problem of cartilaginous tissue, the while has also reduced the mutual displacement after the complex tissue implantation or has come off.(2), can stop the free migration of seed cell in the different tissues by sprayed biological protein gel between two kinds of organizational interfaces.(3) can realize external tissue engineered bone and the tissue engineering bone/cartilage of making up respectively, carry out external assembling again, thereby reduce the structure difficulty of complex tissue.(4) raw material obtains easily, and the back immunogenicity that implants is extremely low.Adopting the complex tissue of this interface in conjunction with structure construction, is the substitute of the biologically active of a kind of bone that can satisfy clinical requirement and cartilage.The method of tissue engineered bone provided by the invention and tissue engineering bone/cartilage complex tissue external structure, adapt with the structure of this complex tissue, have following advantage: (1) obtains concaveconvex structure by timbering material being carried out the interface pretreatment, avoided when complex tissue cutting to engineering tissue, thereby the structural intergrity that has kept the tissue of structure, and because be that timbering material is carried out surface preparation, with respect to concerning engineering tissue itself is handled, being more prone to.(2) by external structure single organization, and then carry out external assembling, make that structure work is easier to be carried out.(3) make later implant surgery become simpler, wound is little, thereby has established solid foundation for corresponding clinical transplantation work.
Description of drawings
Fig. 1 is a kind of embodiment figure of tissue engineered bone of the present invention and cartilage complex tissue;
Fig. 2 is the another kind of embodiment figure of tissue engineered bone of the present invention and cartilage complex tissue.
The specific embodiment
Be described further below in conjunction with accompanying drawing and tool present embodiment integrated structure and external structure method thereof, to help understanding content of the present invention tissue engineered bone of the present invention and cartilage complex tissue.
Embodiment 1
Tissue engineered bone and cartilage complex tissue relate to organizational project osseous tissue and tissue engineering bone/cartilage tissue, and the groove and the bulge-structure embedded structure of concavo-convex relationship are entrenched togather each other by the junction, interface for organizational project osseous tissue and tissue engineering bone/cartilage tissue.The groove and the bulge-structure of combination interface can be as depicted in figs. 1 and 2, rectangle or trapezoidal projection are arranged on tissue engineered bone 1, have on the tissue engineering bone/cartilage 3 with tissue engineered bone 1 mating surface on rectangle or trapezoidal the projection rectangle or the trapezoidal groove that match, the groove of two kinds of tissues and projection be chimeric being assembled together mutually, and bioprotein gel layer 2 is arranged in the combination interface of two kinds of tissues.Bioprotein gel layer 2 is except that stopping that different seed cells by the free migration between two kinds of tissues at the interface, can also increase the bonded fastness of engineering osseous tissue and engineered cartilage tissue.The shape of two kinds of structural grooves and projection also can be other shape, as long as reach being entrenched togather that both can be firm.Groove both can be as depicted in figs. 1 and 2, be arranged on the faying face of organizational project soft 3, also can be arranged on the faying face of tissue engineered bone 1, perhaps the mating surface at a tissue is provided with groove and bulge-structure simultaneously, and the quantity of groove and projection also is not limited to one.
What present embodiment related to is the method for tissue engineered bone and tissue engineering bone/cartilage complex tissue external structure, and this method comprises following process:
(1) external preparation is used for the timbering material of tissue construction: with the timbering material that can be used in tissue engineered bone or tissue engineering bone/cartilage of mould oneself preparation or purchase required form, carry out the interface pretreatment, the interface that bone tissue engineering scaffold and tissue engineering bone/cartilage timbering material are mutually combined becomes the groove and the bulge-structure interface of concavo-convex relationship each other; Its bonded concaveconvex structure can be as shown in Fig. 1 and Fig. 2 shape.
(2) the external independent structure of tissue engineered bone and tissue engineering bone/cartilage: the structure means of utilizing existing tissue engineered bone and tissue engineering bone/cartilage, with to become the cartilage seed cell compound with corresponding bone tissue engineering scaffold and tissue engineering bone/cartilage timbering material respectively, adopt existing means to carry out static state or dynamically cultivate then the skeletonization seed cell of external a large amount of amplifications external.
(3) with the combination interface sprayed biological protein gel of cultured tissue engineered bone and tissue engineering bone/cartilage, utilize its each other the tongue and groove of concavo-convex relationship assembly together, form complex tissue, transplant waiting.
Claims (2)
1. tissue engineered bone and cartilage complex tissue, comprise tissue engineered bone and tissue engineering bone/cartilage, it is characterized in that: described tissue engineered bone and tissue engineering bone/cartilage by the combination interface place each other the groove and the projection of concavo-convex relationship be entrenched togather, between two kinds of organizational interfaces, be coated with the bioprotein gel.
2. tissue engineered bone and tissue engineering bone/cartilage complex tissue external structure method, this method comprises following process:
(1) external preparation is used for the timbering material of tissue construction: with mould preparation or purchase timbering material, carry out the interface pretreatment, the interface that bone tissue engineering scaffold and tissue engineering bone/cartilage timbering material are mutually combined becomes the groove and the bulge-structure of concavo-convex relationship each other;
(2) the external independent structure of tissue engineered bone and tissue engineering bone/cartilage: the skeletonization seed cell of external a large amount of amplifications with to become the cartilage seed cell compound with corresponding bone tissue engineering scaffold and tissue engineering bone/cartilage timbering material respectively, carried out static state or dynamically cultivates external then;
(3) with the combination interface sprayed biological protein gel of cultured tissue engineered bone and two kinds of tissues of tissue engineering bone/cartilage, utilize its groove and bulge-structure assembling of concavo-convex relationship each other to be entrenched togather, form complex tissue.
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| CNB2005100019687A CN100384390C (en) | 2005-01-13 | 2005-01-13 | Complex tissue of tissue-engineered bone and cartilage and external constructing method thereof |
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| CNB2005100019687A CN100384390C (en) | 2005-01-13 | 2005-01-13 | Complex tissue of tissue-engineered bone and cartilage and external constructing method thereof |
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| CN100384390C true CN100384390C (en) | 2008-04-30 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101816806B (en) * | 2010-02-05 | 2013-04-17 | 余方圆 | Cartilage complex of tissue engineering bone and method for preparing same |
| CN101822851B (en) * | 2010-06-21 | 2013-07-03 | 余方圆 | Preparation method of tissue engineered bone cartilage frame |
| CN104593259A (en) * | 2015-02-12 | 2015-05-06 | 中国人民解放军第三军医大学第一附属医院 | Tissue-engineered bone and cartilage double-chamber cultivation system |
| CN105435311B (en) * | 2015-12-11 | 2018-04-24 | 西南交通大学 | A kind of tissue engineered bone cartilage compound rest and preparation method thereof |
| CN107669371B (en) * | 2017-08-02 | 2018-08-03 | 中南大学湘雅医院 | Three-phase contexture engineering rack |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997045147A1 (en) * | 1996-05-28 | 1997-12-04 | 1218122 Ontario Inc. | Resorbable implant biomaterial made of condensed calcium phosphate particles |
| US6242247B1 (en) * | 1996-06-04 | 2001-06-05 | Sulzer Orthopedics Ltd. | Method for making cartilage and implants |
| US6283980B1 (en) * | 1996-08-30 | 2001-09-04 | Verigen Transplantation Services Internt'l | Method, instruments, and kit for autologous transplantation |
| CN1169494C (en) * | 2001-05-23 | 2004-10-06 | 中国科学院化学研究所 | Cell scaffold with composite structure for tissue engineering and its preparing process and application |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997045147A1 (en) * | 1996-05-28 | 1997-12-04 | 1218122 Ontario Inc. | Resorbable implant biomaterial made of condensed calcium phosphate particles |
| US6242247B1 (en) * | 1996-06-04 | 2001-06-05 | Sulzer Orthopedics Ltd. | Method for making cartilage and implants |
| US6283980B1 (en) * | 1996-08-30 | 2001-09-04 | Verigen Transplantation Services Internt'l | Method, instruments, and kit for autologous transplantation |
| CN1169494C (en) * | 2001-05-23 | 2004-10-06 | 中国科学院化学研究所 | Cell scaffold with composite structure for tissue engineering and its preparing process and application |
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